pclipf vectors  (New England Biolabs)


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    Name:
    pCLIPf Vector
    Description:
    pCLIPf Vector 20 ug
    Catalog Number:
    n9215s
    Price:
    166
    Size:
    20 ug
    Category:
    Vectors Plasmids
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    Structured Review

    New England Biolabs pclipf vectors
    pCLIPf Vector
    pCLIPf Vector 20 ug
    https://www.bioz.com/result/pclipf vectors/product/New England Biolabs
    Average 97 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    pclipf vectors - by Bioz Stars, 2020-08
    97/100 stars

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    Related Articles

    Clone Assay:

    Article Title: WDR76 Co-Localizes with Heterochromatin Related Proteins and Rapidly Responds to DNA Damage
    Article Snippet: .. Selected ORFs coding for PARP1, XRCC5, RPA1, CBX1, CBX3, and CBX5 were cloned into a modified pCLIP-tag™-FLAG vector. pCLIP-FLAG was constructed by inserting a DNA fragment encoding a FLAG tag followed by the restriction site Sgf1 into the multiple cloning site of the pCLIP-tag™ vector from New England Biolabs (Ipswich, MA). .. We also constructed CLIP-WDR76 and Halo-RPA1 vectors for use in a reverse tag imaging experiment.

    Article Title: Rapid degradation of progressive ankylosis protein (ANKH) in craniometaphyseal dysplasia
    Article Snippet: .. Wt and mutant Ank clones were subcloned N-terminal of pSNAPf and pCLIPf (NEB) mammalian expression vectors using forward primer 5′-GGCGCGCCACCATGG ACTACAAA-3′ containing a restriction site for AscI and a Kozak sequence GCCACC and a reverse primer 5′-TCCGGAATTCCTCATTTT CTTCTCTCATC-3′ containing an EcoRI restriction site without stop codon. .. These vectors contained SNAP and CLIP-tags.

    Article Title: A heterotrimer model of the complete Microprocessor complex revealed by single-molecule subunit counting
    Article Snippet: .. pSNAP-tag(m) and pCLIP-tag(m) vectors were purchased from NEB. pSNAP-DGCR8: A thrombin site was cloned into the BamHI site of pSNAP-tag(m) to give pSNAP-thrombin. ..

    Article Title: Unprecedentedly efficient CUG initiation of an overlapping reading frame in POLG mRNA yields novel protein POLGARF
    Article Snippet: .. To create constructs with SNAP and CLIP tags, the pGEX6p1-POLGARF and pGEX6p1-P32 were digested with BamH1 and NotI and cloned into pSNAPf and pCLIPf vectors from New England Biolabs (NEB, Ipswich, MA) to create pSNAPf-POLGARF, pCLIPf-POLGARF, pSNAPf-P32 and pCLIPf-P32, respectively. .. These constructs were further re-cloned into pcDNA3.4 (Spe1-Not1 digestion) to create pcDNA3.4-SNAP-POLGARF, pcDNA3.4-CLIP-POLGARF, pcDNA3.4-SNAP-P32 and pcDNA3.4-CLIP-P32, respectively.

    Article Title: Newly synthesized claudins but not occludin are added to the basal side of the tight junction
    Article Snippet: .. In some experiments, stable cell lines overexpressing SNAP and CLIP-tagged cldns and mutants were generated by PCR, using pTRE human cldn4 ( ) as a template and cloning into the Bam HI/Xh oI sites in pSNAPf and pCLIPf (New England Biolabs, N9215S). .. SNAP ocln construct was made by PCR from EGFP ocln ( ).

    Amplification:

    Article Title: Serine Phosphorylation of the Hepatitis C Virus NS5A Protein Controls the Establishment of Replication Complexes
    Article Snippet: .. For the insertion of the SNAP and CLIP tags into NS5A, the gene was amplified from the pSNAPf and pCLIPf vectors (NEB) using primers that insert a flexible linker sequence (GSSGSS) on either side of the insertion (primer sequences are available upon request). .. This amplicon was inserted into the aforementioned LITMUS28i subclone by BclI digestion, and correct insertion was verified by sequencing.

    Stable Transfection:

    Article Title: Newly synthesized claudins but not occludin are added to the basal side of the tight junction
    Article Snippet: .. In some experiments, stable cell lines overexpressing SNAP and CLIP-tagged cldns and mutants were generated by PCR, using pTRE human cldn4 ( ) as a template and cloning into the Bam HI/Xh oI sites in pSNAPf and pCLIPf (New England Biolabs, N9215S). .. SNAP ocln construct was made by PCR from EGFP ocln ( ).

    FLAG-tag:

    Article Title: WDR76 Co-Localizes with Heterochromatin Related Proteins and Rapidly Responds to DNA Damage
    Article Snippet: .. Selected ORFs coding for PARP1, XRCC5, RPA1, CBX1, CBX3, and CBX5 were cloned into a modified pCLIP-tag™-FLAG vector. pCLIP-FLAG was constructed by inserting a DNA fragment encoding a FLAG tag followed by the restriction site Sgf1 into the multiple cloning site of the pCLIP-tag™ vector from New England Biolabs (Ipswich, MA). .. We also constructed CLIP-WDR76 and Halo-RPA1 vectors for use in a reverse tag imaging experiment.

    Mutagenesis:

    Article Title: Rapid degradation of progressive ankylosis protein (ANKH) in craniometaphyseal dysplasia
    Article Snippet: .. Wt and mutant Ank clones were subcloned N-terminal of pSNAPf and pCLIPf (NEB) mammalian expression vectors using forward primer 5′-GGCGCGCCACCATGG ACTACAAA-3′ containing a restriction site for AscI and a Kozak sequence GCCACC and a reverse primer 5′-TCCGGAATTCCTCATTTT CTTCTCTCATC-3′ containing an EcoRI restriction site without stop codon. .. These vectors contained SNAP and CLIP-tags.

    Construct:

    Article Title: WDR76 Co-Localizes with Heterochromatin Related Proteins and Rapidly Responds to DNA Damage
    Article Snippet: .. Selected ORFs coding for PARP1, XRCC5, RPA1, CBX1, CBX3, and CBX5 were cloned into a modified pCLIP-tag™-FLAG vector. pCLIP-FLAG was constructed by inserting a DNA fragment encoding a FLAG tag followed by the restriction site Sgf1 into the multiple cloning site of the pCLIP-tag™ vector from New England Biolabs (Ipswich, MA). .. We also constructed CLIP-WDR76 and Halo-RPA1 vectors for use in a reverse tag imaging experiment.

    Article Title: Unprecedentedly efficient CUG initiation of an overlapping reading frame in POLG mRNA yields novel protein POLGARF
    Article Snippet: .. To create constructs with SNAP and CLIP tags, the pGEX6p1-POLGARF and pGEX6p1-P32 were digested with BamH1 and NotI and cloned into pSNAPf and pCLIPf vectors from New England Biolabs (NEB, Ipswich, MA) to create pSNAPf-POLGARF, pCLIPf-POLGARF, pSNAPf-P32 and pCLIPf-P32, respectively. .. These constructs were further re-cloned into pcDNA3.4 (Spe1-Not1 digestion) to create pcDNA3.4-SNAP-POLGARF, pcDNA3.4-CLIP-POLGARF, pcDNA3.4-SNAP-P32 and pcDNA3.4-CLIP-P32, respectively.

    Article Title: Cbl-mediated K63-linked ubiquitination of JAK2 enhances JAK2 phosphorylation and signal transduction
    Article Snippet: .. Plasmids pcDNA3-GMRα and pGMRβ-clip are pcDNA3 vector-based expression systems encoding GMRα and GMRβ-clip proteins, respectively, as previously described . pGMRβ-clip was constructed by inserting the PCR-amplified clip coding region from pCLIP vector (New England BioLabs,Ipswich, MA, USA) into the C terminus of the GMRβ gene in pCDNA3-GMRβ. .. The Cbl cDNA was kindly provided by Dr. Wallace Y. Langdon (School of Pathology and Laboratory Medicine, University of Western Australia, Australia), and sub-cloned into the pcDNA6 vector.

    Sequencing:

    Article Title: Serine Phosphorylation of the Hepatitis C Virus NS5A Protein Controls the Establishment of Replication Complexes
    Article Snippet: .. For the insertion of the SNAP and CLIP tags into NS5A, the gene was amplified from the pSNAPf and pCLIPf vectors (NEB) using primers that insert a flexible linker sequence (GSSGSS) on either side of the insertion (primer sequences are available upon request). .. This amplicon was inserted into the aforementioned LITMUS28i subclone by BclI digestion, and correct insertion was verified by sequencing.

    Article Title: Rapid degradation of progressive ankylosis protein (ANKH) in craniometaphyseal dysplasia
    Article Snippet: .. Wt and mutant Ank clones were subcloned N-terminal of pSNAPf and pCLIPf (NEB) mammalian expression vectors using forward primer 5′-GGCGCGCCACCATGG ACTACAAA-3′ containing a restriction site for AscI and a Kozak sequence GCCACC and a reverse primer 5′-TCCGGAATTCCTCATTTT CTTCTCTCATC-3′ containing an EcoRI restriction site without stop codon. .. These vectors contained SNAP and CLIP-tags.

    Generated:

    Article Title: Newly synthesized claudins but not occludin are added to the basal side of the tight junction
    Article Snippet: .. In some experiments, stable cell lines overexpressing SNAP and CLIP-tagged cldns and mutants were generated by PCR, using pTRE human cldn4 ( ) as a template and cloning into the Bam HI/Xh oI sites in pSNAPf and pCLIPf (New England Biolabs, N9215S). .. SNAP ocln construct was made by PCR from EGFP ocln ( ).

    Expressing:

    Article Title: Rapid degradation of progressive ankylosis protein (ANKH) in craniometaphyseal dysplasia
    Article Snippet: .. Wt and mutant Ank clones were subcloned N-terminal of pSNAPf and pCLIPf (NEB) mammalian expression vectors using forward primer 5′-GGCGCGCCACCATGG ACTACAAA-3′ containing a restriction site for AscI and a Kozak sequence GCCACC and a reverse primer 5′-TCCGGAATTCCTCATTTT CTTCTCTCATC-3′ containing an EcoRI restriction site without stop codon. .. These vectors contained SNAP and CLIP-tags.

    Article Title: Cbl-mediated K63-linked ubiquitination of JAK2 enhances JAK2 phosphorylation and signal transduction
    Article Snippet: .. Plasmids pcDNA3-GMRα and pGMRβ-clip are pcDNA3 vector-based expression systems encoding GMRα and GMRβ-clip proteins, respectively, as previously described . pGMRβ-clip was constructed by inserting the PCR-amplified clip coding region from pCLIP vector (New England BioLabs,Ipswich, MA, USA) into the C terminus of the GMRβ gene in pCDNA3-GMRβ. .. The Cbl cDNA was kindly provided by Dr. Wallace Y. Langdon (School of Pathology and Laboratory Medicine, University of Western Australia, Australia), and sub-cloned into the pcDNA6 vector.

    Modification:

    Article Title: WDR76 Co-Localizes with Heterochromatin Related Proteins and Rapidly Responds to DNA Damage
    Article Snippet: .. Selected ORFs coding for PARP1, XRCC5, RPA1, CBX1, CBX3, and CBX5 were cloned into a modified pCLIP-tag™-FLAG vector. pCLIP-FLAG was constructed by inserting a DNA fragment encoding a FLAG tag followed by the restriction site Sgf1 into the multiple cloning site of the pCLIP-tag™ vector from New England Biolabs (Ipswich, MA). .. We also constructed CLIP-WDR76 and Halo-RPA1 vectors for use in a reverse tag imaging experiment.

    Polymerase Chain Reaction:

    Article Title: Cbl-mediated K63-linked ubiquitination of JAK2 enhances JAK2 phosphorylation and signal transduction
    Article Snippet: .. Plasmids pcDNA3-GMRα and pGMRβ-clip are pcDNA3 vector-based expression systems encoding GMRα and GMRβ-clip proteins, respectively, as previously described . pGMRβ-clip was constructed by inserting the PCR-amplified clip coding region from pCLIP vector (New England BioLabs,Ipswich, MA, USA) into the C terminus of the GMRβ gene in pCDNA3-GMRβ. .. The Cbl cDNA was kindly provided by Dr. Wallace Y. Langdon (School of Pathology and Laboratory Medicine, University of Western Australia, Australia), and sub-cloned into the pcDNA6 vector.

    Article Title: Newly synthesized claudins but not occludin are added to the basal side of the tight junction
    Article Snippet: .. In some experiments, stable cell lines overexpressing SNAP and CLIP-tagged cldns and mutants were generated by PCR, using pTRE human cldn4 ( ) as a template and cloning into the Bam HI/Xh oI sites in pSNAPf and pCLIPf (New England Biolabs, N9215S). .. SNAP ocln construct was made by PCR from EGFP ocln ( ).

    Cross-linking Immunoprecipitation:

    Article Title: Serine Phosphorylation of the Hepatitis C Virus NS5A Protein Controls the Establishment of Replication Complexes
    Article Snippet: .. For the insertion of the SNAP and CLIP tags into NS5A, the gene was amplified from the pSNAPf and pCLIPf vectors (NEB) using primers that insert a flexible linker sequence (GSSGSS) on either side of the insertion (primer sequences are available upon request). .. This amplicon was inserted into the aforementioned LITMUS28i subclone by BclI digestion, and correct insertion was verified by sequencing.

    Article Title: Unprecedentedly efficient CUG initiation of an overlapping reading frame in POLG mRNA yields novel protein POLGARF
    Article Snippet: .. To create constructs with SNAP and CLIP tags, the pGEX6p1-POLGARF and pGEX6p1-P32 were digested with BamH1 and NotI and cloned into pSNAPf and pCLIPf vectors from New England Biolabs (NEB, Ipswich, MA) to create pSNAPf-POLGARF, pCLIPf-POLGARF, pSNAPf-P32 and pCLIPf-P32, respectively. .. These constructs were further re-cloned into pcDNA3.4 (Spe1-Not1 digestion) to create pcDNA3.4-SNAP-POLGARF, pcDNA3.4-CLIP-POLGARF, pcDNA3.4-SNAP-P32 and pcDNA3.4-CLIP-P32, respectively.

    Article Title: Cbl-mediated K63-linked ubiquitination of JAK2 enhances JAK2 phosphorylation and signal transduction
    Article Snippet: .. Plasmids pcDNA3-GMRα and pGMRβ-clip are pcDNA3 vector-based expression systems encoding GMRα and GMRβ-clip proteins, respectively, as previously described . pGMRβ-clip was constructed by inserting the PCR-amplified clip coding region from pCLIP vector (New England BioLabs,Ipswich, MA, USA) into the C terminus of the GMRβ gene in pCDNA3-GMRβ. .. The Cbl cDNA was kindly provided by Dr. Wallace Y. Langdon (School of Pathology and Laboratory Medicine, University of Western Australia, Australia), and sub-cloned into the pcDNA6 vector.

    Article Title: Newly synthesized claudins but not occludin are added to the basal side of the tight junction
    Article Snippet: .. In some experiments, stable cell lines overexpressing SNAP and CLIP-tagged cldns and mutants were generated by PCR, using pTRE human cldn4 ( ) as a template and cloning into the Bam HI/Xh oI sites in pSNAPf and pCLIPf (New England Biolabs, N9215S). .. SNAP ocln construct was made by PCR from EGFP ocln ( ).

    Article Title: N-glycosylation of α1D-adrenergic receptor N-terminal domain is required for correct trafficking, function, and biogenesis
    Article Snippet: .. The pSNAPf and pCLIP vector, as well as SNAP substrates, BG-782 and Alexa Fluor 488, and CLIP substrate, BC-680 were purchased from New England Biolabs (Ipswich, MA). .. PageRuler Prestained NIR Protein Ladder was used for all PAGE NIR analyses (Thermo Fisher Scientific, Waltham, MA).

    Plasmid Preparation:

    Article Title: WDR76 Co-Localizes with Heterochromatin Related Proteins and Rapidly Responds to DNA Damage
    Article Snippet: .. Selected ORFs coding for PARP1, XRCC5, RPA1, CBX1, CBX3, and CBX5 were cloned into a modified pCLIP-tag™-FLAG vector. pCLIP-FLAG was constructed by inserting a DNA fragment encoding a FLAG tag followed by the restriction site Sgf1 into the multiple cloning site of the pCLIP-tag™ vector from New England Biolabs (Ipswich, MA). .. We also constructed CLIP-WDR76 and Halo-RPA1 vectors for use in a reverse tag imaging experiment.

    Article Title: Cbl-mediated K63-linked ubiquitination of JAK2 enhances JAK2 phosphorylation and signal transduction
    Article Snippet: .. Plasmids pcDNA3-GMRα and pGMRβ-clip are pcDNA3 vector-based expression systems encoding GMRα and GMRβ-clip proteins, respectively, as previously described . pGMRβ-clip was constructed by inserting the PCR-amplified clip coding region from pCLIP vector (New England BioLabs,Ipswich, MA, USA) into the C terminus of the GMRβ gene in pCDNA3-GMRβ. .. The Cbl cDNA was kindly provided by Dr. Wallace Y. Langdon (School of Pathology and Laboratory Medicine, University of Western Australia, Australia), and sub-cloned into the pcDNA6 vector.

    Article Title: N-glycosylation of α1D-adrenergic receptor N-terminal domain is required for correct trafficking, function, and biogenesis
    Article Snippet: .. The pSNAPf and pCLIP vector, as well as SNAP substrates, BG-782 and Alexa Fluor 488, and CLIP substrate, BC-680 were purchased from New England Biolabs (Ipswich, MA). .. PageRuler Prestained NIR Protein Ladder was used for all PAGE NIR analyses (Thermo Fisher Scientific, Waltham, MA).

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  • 97
    New England Biolabs pclipf vectors
    Pclipf Vectors, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pclipf vectors/product/New England Biolabs
    Average 97 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    pclipf vectors - by Bioz Stars, 2020-08
    97/100 stars
      Buy from Supplier

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