pclipf  (New England Biolabs)


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    Name:
    pCLIPf Vector
    Description:
    pCLIPf Vector 20 ug
    Catalog Number:
    N9215S
    Price:
    166
    Category:
    Vectors Plasmids
    Size:
    20 ug
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    New England Biolabs pclipf
    pCLIPf Vector
    pCLIPf Vector 20 ug
    https://www.bioz.com/result/pclipf/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pclipf - by Bioz Stars, 2021-04
    93/100 stars

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    Related Articles

    Clone Assay:

    Article Title: WDR76 Co-Localizes with Heterochromatin Related Proteins and Rapidly Responds to DNA Damage
    Article Snippet: Histone H2B, H2A and H4 ORFs were transferred from the original Flexi® vector pFC14K (C-terminal HaloTag™ expression vector), into the pcDNA5/FRT vector, using the restriction sites NheI/NotI. .. Selected ORFs coding for PARP1, XRCC5, RPA1, CBX1, CBX3, and CBX5 were cloned into a modified pCLIP-tag™-FLAG vector. pCLIP-FLAG was constructed by inserting a DNA fragment encoding a FLAG tag followed by the restriction site Sgf1 into the multiple cloning site of the pCLIP-tag™ vector from New England Biolabs (Ipswich, MA). .. We also constructed CLIP-WDR76 and Halo-RPA1 vectors for use in a reverse tag imaging experiment.

    Article Title: The Interaction of RecA With Both CheA and CheW Is Required for Chemotaxis
    Article Snippet: The membranes were imaged using a ChemiDocTM XRS + system (Bio-Rad). .. Construction of S. enterica Mutant and Tagged Strains S. enterica ΔcheA and S. enterica ΔcheA ΔcheW mutants and -SNAP and/or -CLIP tagged strains were constructed according to the λRed recombinase-based gene replacement method ( ; ). pGEM-T vectors (Promega) containing -SNAP or -CLIP tags from pSNAP-tag (T7)-2 and pCLIPf vectors (NEB) followed by the kanamycin cassette from pKD4 vector ( ) were constructed using HiFi DNA assembly cloning kit (NEB) giving rise to pUA1135 and pUA1136, respectively ( ). .. These plasmids were used as the template to amplify the -SNAP or -CLIP tag followed by the kanamycin cassette using the suitable oligonucleotides ( ).

    Article Title: Rapid degradation of progressive ankylosis protein (ANKH) in craniometaphyseal dysplasia
    Article Snippet: F377del and S375del mutant Ank clones were created using a high-fidelity Taq polymerase (New England Biolabs). .. Wt and mutant Ank clones were subcloned N-terminal of pSNAPf and pCLIPf (NEB) mammalian expression vectors using forward primer 5′-GGCGCGCCACCATGG ACTACAAA-3′ containing a restriction site for AscI and a Kozak sequence GCCACC and a reverse primer 5′-TCCGGAATTCCTCATTTT CTTCTCTCATC-3′ containing an EcoRI restriction site without stop codon. .. These vectors contained SNAP and CLIP-tags.

    Article Title: A heterotrimer model of the complete Microprocessor complex revealed by single-molecule subunit counting
    Article Snippet: .. pSNAP-tag(m) and pCLIP-tag(m) vectors were purchased from NEB. pSNAP-DGCR8: A thrombin site was cloned into the BamHI site of pSNAP-tag(m) to give pSNAP-thrombin. ..

    Article Title: Rapid degradation of progressive ankylosis protein (ANKH) in craniometaphyseal dysplasia
    Article Snippet: F377del and S375del mutant Ank clones were created using a high-fidelity Taq polymerase (New England Biolabs). .. Wt and mutant Ank clones were subcloned N-terminal of pSNAPf and pCLIPf (NEB) mammalian expression vectors using forward primer 5′-GGCGCGCCACCATGG ACTACAAA-3′ containing a restriction site for AscI and a Kozak sequence GCCACC and a reverse primer 5′-TCCGGAATTCCTCATTTT CTTCTCTCATC-3′ containing an EcoRI restriction site without stop codon. .. These vectors contained SNAP and CLIP-tags.

    Modification:

    Article Title: WDR76 Co-Localizes with Heterochromatin Related Proteins and Rapidly Responds to DNA Damage
    Article Snippet: Histone H2B, H2A and H4 ORFs were transferred from the original Flexi® vector pFC14K (C-terminal HaloTag™ expression vector), into the pcDNA5/FRT vector, using the restriction sites NheI/NotI. .. Selected ORFs coding for PARP1, XRCC5, RPA1, CBX1, CBX3, and CBX5 were cloned into a modified pCLIP-tag™-FLAG vector. pCLIP-FLAG was constructed by inserting a DNA fragment encoding a FLAG tag followed by the restriction site Sgf1 into the multiple cloning site of the pCLIP-tag™ vector from New England Biolabs (Ipswich, MA). .. We also constructed CLIP-WDR76 and Halo-RPA1 vectors for use in a reverse tag imaging experiment.

    Plasmid Preparation:

    Article Title: WDR76 Co-Localizes with Heterochromatin Related Proteins and Rapidly Responds to DNA Damage
    Article Snippet: Histone H2B, H2A and H4 ORFs were transferred from the original Flexi® vector pFC14K (C-terminal HaloTag™ expression vector), into the pcDNA5/FRT vector, using the restriction sites NheI/NotI. .. Selected ORFs coding for PARP1, XRCC5, RPA1, CBX1, CBX3, and CBX5 were cloned into a modified pCLIP-tag™-FLAG vector. pCLIP-FLAG was constructed by inserting a DNA fragment encoding a FLAG tag followed by the restriction site Sgf1 into the multiple cloning site of the pCLIP-tag™ vector from New England Biolabs (Ipswich, MA). .. We also constructed CLIP-WDR76 and Halo-RPA1 vectors for use in a reverse tag imaging experiment.

    Article Title: The Interaction of RecA With Both CheA and CheW Is Required for Chemotaxis
    Article Snippet: The membranes were imaged using a ChemiDocTM XRS + system (Bio-Rad). .. Construction of S. enterica Mutant and Tagged Strains S. enterica ΔcheA and S. enterica ΔcheA ΔcheW mutants and -SNAP and/or -CLIP tagged strains were constructed according to the λRed recombinase-based gene replacement method ( ; ). pGEM-T vectors (Promega) containing -SNAP or -CLIP tags from pSNAP-tag (T7)-2 and pCLIPf vectors (NEB) followed by the kanamycin cassette from pKD4 vector ( ) were constructed using HiFi DNA assembly cloning kit (NEB) giving rise to pUA1135 and pUA1136, respectively ( ). .. These plasmids were used as the template to amplify the -SNAP or -CLIP tag followed by the kanamycin cassette using the suitable oligonucleotides ( ).

    Article Title: N-glycosylation of α1D-adrenergic receptor N-terminal domain is required for correct trafficking, function, and biogenesis.
    Article Snippet: Molecular cloning was performed using inFusion HD cloning technology (Clontech/Takara Biotech, Mountain View, CA). .. The pSNAPf and pCLIP vector, as well as SNAP substrates, BG-782 and Alexa Fluor 488, and CLIP substrate, BC-680 were purchased from New England Biolabs (Ipswich, MA). .. PageRuler Prestained NIR Protein Ladder was used for all PAGE NIR analyses (Thermo Fisher Scientific, Waltham, MA). cell culture.

    Construct:

    Article Title: WDR76 Co-Localizes with Heterochromatin Related Proteins and Rapidly Responds to DNA Damage
    Article Snippet: Histone H2B, H2A and H4 ORFs were transferred from the original Flexi® vector pFC14K (C-terminal HaloTag™ expression vector), into the pcDNA5/FRT vector, using the restriction sites NheI/NotI. .. Selected ORFs coding for PARP1, XRCC5, RPA1, CBX1, CBX3, and CBX5 were cloned into a modified pCLIP-tag™-FLAG vector. pCLIP-FLAG was constructed by inserting a DNA fragment encoding a FLAG tag followed by the restriction site Sgf1 into the multiple cloning site of the pCLIP-tag™ vector from New England Biolabs (Ipswich, MA). .. We also constructed CLIP-WDR76 and Halo-RPA1 vectors for use in a reverse tag imaging experiment.

    Article Title: The Interaction of RecA With Both CheA and CheW Is Required for Chemotaxis
    Article Snippet: The membranes were imaged using a ChemiDocTM XRS + system (Bio-Rad). .. Construction of S. enterica Mutant and Tagged Strains S. enterica ΔcheA and S. enterica ΔcheA ΔcheW mutants and -SNAP and/or -CLIP tagged strains were constructed according to the λRed recombinase-based gene replacement method ( ; ). pGEM-T vectors (Promega) containing -SNAP or -CLIP tags from pSNAP-tag (T7)-2 and pCLIPf vectors (NEB) followed by the kanamycin cassette from pKD4 vector ( ) were constructed using HiFi DNA assembly cloning kit (NEB) giving rise to pUA1135 and pUA1136, respectively ( ). .. These plasmids were used as the template to amplify the -SNAP or -CLIP tag followed by the kanamycin cassette using the suitable oligonucleotides ( ).

    FLAG-tag:

    Article Title: WDR76 Co-Localizes with Heterochromatin Related Proteins and Rapidly Responds to DNA Damage
    Article Snippet: Histone H2B, H2A and H4 ORFs were transferred from the original Flexi® vector pFC14K (C-terminal HaloTag™ expression vector), into the pcDNA5/FRT vector, using the restriction sites NheI/NotI. .. Selected ORFs coding for PARP1, XRCC5, RPA1, CBX1, CBX3, and CBX5 were cloned into a modified pCLIP-tag™-FLAG vector. pCLIP-FLAG was constructed by inserting a DNA fragment encoding a FLAG tag followed by the restriction site Sgf1 into the multiple cloning site of the pCLIP-tag™ vector from New England Biolabs (Ipswich, MA). .. We also constructed CLIP-WDR76 and Halo-RPA1 vectors for use in a reverse tag imaging experiment.

    Amplification:

    Article Title: Ultrafast tissue staining with chemical tags
    Article Snippet: CLIPf and SNAPf are engineered versions of the original versions CLIP26m (CLIPm) and SNAP26m (SNAPm), respectively, that display faster labeling kinetics ( , ). pTW is a pUASt vector containing a Gateway cassette. .. All CLIPm, CLIPf, SNAPm, and SNAPf coding sequences were amplified from pCLIPm, pCLIPf, pSNAPm, or pSNAPf plamids (New England Biolabs), respectively. .. Drosophila synaptotagmin 1 ( syt1 ; GenBank accession no. ) was amplified from P{UAS-syt.eGFP}1 flies (Bloomington Stock Center) ( ) using syt forward and eGFP reverse primers.

    Mutagenesis:

    Article Title: The Interaction of RecA With Both CheA and CheW Is Required for Chemotaxis
    Article Snippet: The membranes were imaged using a ChemiDocTM XRS + system (Bio-Rad). .. Construction of S. enterica Mutant and Tagged Strains S. enterica ΔcheA and S. enterica ΔcheA ΔcheW mutants and -SNAP and/or -CLIP tagged strains were constructed according to the λRed recombinase-based gene replacement method ( ; ). pGEM-T vectors (Promega) containing -SNAP or -CLIP tags from pSNAP-tag (T7)-2 and pCLIPf vectors (NEB) followed by the kanamycin cassette from pKD4 vector ( ) were constructed using HiFi DNA assembly cloning kit (NEB) giving rise to pUA1135 and pUA1136, respectively ( ). .. These plasmids were used as the template to amplify the -SNAP or -CLIP tag followed by the kanamycin cassette using the suitable oligonucleotides ( ).

    Article Title: Rapid degradation of progressive ankylosis protein (ANKH) in craniometaphyseal dysplasia
    Article Snippet: F377del and S375del mutant Ank clones were created using a high-fidelity Taq polymerase (New England Biolabs). .. Wt and mutant Ank clones were subcloned N-terminal of pSNAPf and pCLIPf (NEB) mammalian expression vectors using forward primer 5′-GGCGCGCCACCATGG ACTACAAA-3′ containing a restriction site for AscI and a Kozak sequence GCCACC and a reverse primer 5′-TCCGGAATTCCTCATTTT CTTCTCTCATC-3′ containing an EcoRI restriction site without stop codon. .. These vectors contained SNAP and CLIP-tags.

    Article Title: Rapid degradation of progressive ankylosis protein (ANKH) in craniometaphyseal dysplasia
    Article Snippet: F377del and S375del mutant Ank clones were created using a high-fidelity Taq polymerase (New England Biolabs). .. Wt and mutant Ank clones were subcloned N-terminal of pSNAPf and pCLIPf (NEB) mammalian expression vectors using forward primer 5′-GGCGCGCCACCATGG ACTACAAA-3′ containing a restriction site for AscI and a Kozak sequence GCCACC and a reverse primer 5′-TCCGGAATTCCTCATTTT CTTCTCTCATC-3′ containing an EcoRI restriction site without stop codon. .. These vectors contained SNAP and CLIP-tags.

    Cross-linking Immunoprecipitation:

    Article Title: The Interaction of RecA With Both CheA and CheW Is Required for Chemotaxis
    Article Snippet: The membranes were imaged using a ChemiDocTM XRS + system (Bio-Rad). .. Construction of S. enterica Mutant and Tagged Strains S. enterica ΔcheA and S. enterica ΔcheA ΔcheW mutants and -SNAP and/or -CLIP tagged strains were constructed according to the λRed recombinase-based gene replacement method ( ; ). pGEM-T vectors (Promega) containing -SNAP or -CLIP tags from pSNAP-tag (T7)-2 and pCLIPf vectors (NEB) followed by the kanamycin cassette from pKD4 vector ( ) were constructed using HiFi DNA assembly cloning kit (NEB) giving rise to pUA1135 and pUA1136, respectively ( ). .. These plasmids were used as the template to amplify the -SNAP or -CLIP tag followed by the kanamycin cassette using the suitable oligonucleotides ( ).

    Article Title: N-glycosylation of α1D-adrenergic receptor N-terminal domain is required for correct trafficking, function, and biogenesis.
    Article Snippet: Molecular cloning was performed using inFusion HD cloning technology (Clontech/Takara Biotech, Mountain View, CA). .. The pSNAPf and pCLIP vector, as well as SNAP substrates, BG-782 and Alexa Fluor 488, and CLIP substrate, BC-680 were purchased from New England Biolabs (Ipswich, MA). .. PageRuler Prestained NIR Protein Ladder was used for all PAGE NIR analyses (Thermo Fisher Scientific, Waltham, MA). cell culture.

    Expressing:

    Article Title: Rapid degradation of progressive ankylosis protein (ANKH) in craniometaphyseal dysplasia
    Article Snippet: F377del and S375del mutant Ank clones were created using a high-fidelity Taq polymerase (New England Biolabs). .. Wt and mutant Ank clones were subcloned N-terminal of pSNAPf and pCLIPf (NEB) mammalian expression vectors using forward primer 5′-GGCGCGCCACCATGG ACTACAAA-3′ containing a restriction site for AscI and a Kozak sequence GCCACC and a reverse primer 5′-TCCGGAATTCCTCATTTT CTTCTCTCATC-3′ containing an EcoRI restriction site without stop codon. .. These vectors contained SNAP and CLIP-tags.

    Article Title: Rapid degradation of progressive ankylosis protein (ANKH) in craniometaphyseal dysplasia
    Article Snippet: F377del and S375del mutant Ank clones were created using a high-fidelity Taq polymerase (New England Biolabs). .. Wt and mutant Ank clones were subcloned N-terminal of pSNAPf and pCLIPf (NEB) mammalian expression vectors using forward primer 5′-GGCGCGCCACCATGG ACTACAAA-3′ containing a restriction site for AscI and a Kozak sequence GCCACC and a reverse primer 5′-TCCGGAATTCCTCATTTT CTTCTCTCATC-3′ containing an EcoRI restriction site without stop codon. .. These vectors contained SNAP and CLIP-tags.

    Sequencing:

    Article Title: Rapid degradation of progressive ankylosis protein (ANKH) in craniometaphyseal dysplasia
    Article Snippet: F377del and S375del mutant Ank clones were created using a high-fidelity Taq polymerase (New England Biolabs). .. Wt and mutant Ank clones were subcloned N-terminal of pSNAPf and pCLIPf (NEB) mammalian expression vectors using forward primer 5′-GGCGCGCCACCATGG ACTACAAA-3′ containing a restriction site for AscI and a Kozak sequence GCCACC and a reverse primer 5′-TCCGGAATTCCTCATTTT CTTCTCTCATC-3′ containing an EcoRI restriction site without stop codon. .. These vectors contained SNAP and CLIP-tags.

    Article Title: Rapid degradation of progressive ankylosis protein (ANKH) in craniometaphyseal dysplasia
    Article Snippet: F377del and S375del mutant Ank clones were created using a high-fidelity Taq polymerase (New England Biolabs). .. Wt and mutant Ank clones were subcloned N-terminal of pSNAPf and pCLIPf (NEB) mammalian expression vectors using forward primer 5′-GGCGCGCCACCATGG ACTACAAA-3′ containing a restriction site for AscI and a Kozak sequence GCCACC and a reverse primer 5′-TCCGGAATTCCTCATTTT CTTCTCTCATC-3′ containing an EcoRI restriction site without stop codon. .. These vectors contained SNAP and CLIP-tags.

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  • 93
    New England Biolabs pclipf
    Pclipf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pclipf/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pclipf - by Bioz Stars, 2021-04
    93/100 stars
      Buy from Supplier

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