pclipf  (New England Biolabs)


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  • 97
    Name:
    pCLIPf Vector
    Description:
    pCLIPf Vector 20 ug
    Catalog Number:
    N9215S
    Price:
    166
    Category:
    Vectors Plasmids
    Size:
    20 ug
    Buy from Supplier


    Structured Review

    New England Biolabs pclipf
    pCLIPf Vector
    pCLIPf Vector 20 ug
    https://www.bioz.com/result/pclipf/product/New England Biolabs
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pclipf - by Bioz Stars, 2021-07
    97/100 stars

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    Related Articles

    Clone Assay:

    Article Title: WDR76 Co-Localizes with Heterochromatin Related Proteins and Rapidly Responds to DNA Damage
    Article Snippet: Histone H2B, H2A and H4 ORFs were transferred from the original Flexi® vector pFC14K (C-terminal HaloTag™ expression vector), into the pcDNA5/FRT vector, using the restriction sites NheI/NotI. .. Selected ORFs coding for PARP1, XRCC5, RPA1, CBX1, CBX3, and CBX5 were cloned into a modified pCLIP-tag™-FLAG vector. pCLIP-FLAG was constructed by inserting a DNA fragment encoding a FLAG tag followed by the restriction site Sgf1 into the multiple cloning site of the pCLIP-tag™ vector from New England Biolabs (Ipswich, MA). .. We also constructed CLIP-WDR76 and Halo-RPA1 vectors for use in a reverse tag imaging experiment.

    Article Title: Rapid degradation of progressive ankylosis protein (ANKH) in craniometaphyseal dysplasia
    Article Snippet: F377del and S375del mutant Ank clones were created using a high-fidelity Taq polymerase (New England Biolabs). .. Wt and mutant Ank clones were subcloned N-terminal of pSNAPf and pCLIPf (NEB) mammalian expression vectors using forward primer 5′-GGCGCGCCACCATGG ACTACAAA-3′ containing a restriction site for AscI and a Kozak sequence GCCACC and a reverse primer 5′-TCCGGAATTCCTCATTTT CTTCTCTCATC-3′ containing an EcoRI restriction site without stop codon. .. These vectors contained SNAP and CLIP-tags.

    Modification:

    Article Title: WDR76 Co-Localizes with Heterochromatin Related Proteins and Rapidly Responds to DNA Damage
    Article Snippet: Histone H2B, H2A and H4 ORFs were transferred from the original Flexi® vector pFC14K (C-terminal HaloTag™ expression vector), into the pcDNA5/FRT vector, using the restriction sites NheI/NotI. .. Selected ORFs coding for PARP1, XRCC5, RPA1, CBX1, CBX3, and CBX5 were cloned into a modified pCLIP-tag™-FLAG vector. pCLIP-FLAG was constructed by inserting a DNA fragment encoding a FLAG tag followed by the restriction site Sgf1 into the multiple cloning site of the pCLIP-tag™ vector from New England Biolabs (Ipswich, MA). .. We also constructed CLIP-WDR76 and Halo-RPA1 vectors for use in a reverse tag imaging experiment.

    Plasmid Preparation:

    Article Title: WDR76 Co-Localizes with Heterochromatin Related Proteins and Rapidly Responds to DNA Damage
    Article Snippet: Histone H2B, H2A and H4 ORFs were transferred from the original Flexi® vector pFC14K (C-terminal HaloTag™ expression vector), into the pcDNA5/FRT vector, using the restriction sites NheI/NotI. .. Selected ORFs coding for PARP1, XRCC5, RPA1, CBX1, CBX3, and CBX5 were cloned into a modified pCLIP-tag™-FLAG vector. pCLIP-FLAG was constructed by inserting a DNA fragment encoding a FLAG tag followed by the restriction site Sgf1 into the multiple cloning site of the pCLIP-tag™ vector from New England Biolabs (Ipswich, MA). .. We also constructed CLIP-WDR76 and Halo-RPA1 vectors for use in a reverse tag imaging experiment.

    Construct:

    Article Title: WDR76 Co-Localizes with Heterochromatin Related Proteins and Rapidly Responds to DNA Damage
    Article Snippet: Histone H2B, H2A and H4 ORFs were transferred from the original Flexi® vector pFC14K (C-terminal HaloTag™ expression vector), into the pcDNA5/FRT vector, using the restriction sites NheI/NotI. .. Selected ORFs coding for PARP1, XRCC5, RPA1, CBX1, CBX3, and CBX5 were cloned into a modified pCLIP-tag™-FLAG vector. pCLIP-FLAG was constructed by inserting a DNA fragment encoding a FLAG tag followed by the restriction site Sgf1 into the multiple cloning site of the pCLIP-tag™ vector from New England Biolabs (Ipswich, MA). .. We also constructed CLIP-WDR76 and Halo-RPA1 vectors for use in a reverse tag imaging experiment.

    FLAG-tag:

    Article Title: WDR76 Co-Localizes with Heterochromatin Related Proteins and Rapidly Responds to DNA Damage
    Article Snippet: Histone H2B, H2A and H4 ORFs were transferred from the original Flexi® vector pFC14K (C-terminal HaloTag™ expression vector), into the pcDNA5/FRT vector, using the restriction sites NheI/NotI. .. Selected ORFs coding for PARP1, XRCC5, RPA1, CBX1, CBX3, and CBX5 were cloned into a modified pCLIP-tag™-FLAG vector. pCLIP-FLAG was constructed by inserting a DNA fragment encoding a FLAG tag followed by the restriction site Sgf1 into the multiple cloning site of the pCLIP-tag™ vector from New England Biolabs (Ipswich, MA). .. We also constructed CLIP-WDR76 and Halo-RPA1 vectors for use in a reverse tag imaging experiment.

    Mutagenesis:

    Article Title: Rapid degradation of progressive ankylosis protein (ANKH) in craniometaphyseal dysplasia
    Article Snippet: F377del and S375del mutant Ank clones were created using a high-fidelity Taq polymerase (New England Biolabs). .. Wt and mutant Ank clones were subcloned N-terminal of pSNAPf and pCLIPf (NEB) mammalian expression vectors using forward primer 5′-GGCGCGCCACCATGG ACTACAAA-3′ containing a restriction site for AscI and a Kozak sequence GCCACC and a reverse primer 5′-TCCGGAATTCCTCATTTT CTTCTCTCATC-3′ containing an EcoRI restriction site without stop codon. .. These vectors contained SNAP and CLIP-tags.

    Expressing:

    Article Title: Rapid degradation of progressive ankylosis protein (ANKH) in craniometaphyseal dysplasia
    Article Snippet: F377del and S375del mutant Ank clones were created using a high-fidelity Taq polymerase (New England Biolabs). .. Wt and mutant Ank clones were subcloned N-terminal of pSNAPf and pCLIPf (NEB) mammalian expression vectors using forward primer 5′-GGCGCGCCACCATGG ACTACAAA-3′ containing a restriction site for AscI and a Kozak sequence GCCACC and a reverse primer 5′-TCCGGAATTCCTCATTTT CTTCTCTCATC-3′ containing an EcoRI restriction site without stop codon. .. These vectors contained SNAP and CLIP-tags.

    Sequencing:

    Article Title: Rapid degradation of progressive ankylosis protein (ANKH) in craniometaphyseal dysplasia
    Article Snippet: F377del and S375del mutant Ank clones were created using a high-fidelity Taq polymerase (New England Biolabs). .. Wt and mutant Ank clones were subcloned N-terminal of pSNAPf and pCLIPf (NEB) mammalian expression vectors using forward primer 5′-GGCGCGCCACCATGG ACTACAAA-3′ containing a restriction site for AscI and a Kozak sequence GCCACC and a reverse primer 5′-TCCGGAATTCCTCATTTT CTTCTCTCATC-3′ containing an EcoRI restriction site without stop codon. .. These vectors contained SNAP and CLIP-tags.

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  • 93
    New England Biolabs pclip tag vector
    Pclip Tag Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs pclipf vector
    Pclipf Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pclipf vector/product/New England Biolabs
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