psnaptag (New England Biolabs)


97
Name:
pSNAP tag T7 2 Vector
Description:
pSNAP tag T7 2 Vector 20 ug
Catalog Number:
N9181S
Price:
166
Category:
Vectors Plasmids
Size:
20 ug
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Structured Review
New England Biolabs
psnaptag

pSNAP tag T7 2 Vector 20 ug
https://www.bioz.com/result/psnaptag/product/New England Biolabs
Average 97 stars, based on 1 article reviews
Price from $9.99 to $1999.99

pSNAP tag T7 2 Vector 20 ug
https://www.bioz.com/result/psnaptag/product/New England Biolabs
Average 97 stars, based on 1 article reviews
Price from $9.99 to $1999.99
psnaptag - by Bioz Stars,
2021-04
97/100 stars
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Related Articles
Amplification:Article Title: Engineering a living biomaterial via bacterial surface capture of environmental molecules Article Snippet: Promoter PBAD was amplified through polymerase chain reaction (PCR) from plasmid pKLi034. .. The SNAP gene was amplified from Article Title: Protein-Protein Interaction Inhibition (2P2I)-Oriented Chemical Library Accelerates Hit Discovery. Article Snippet: For the cloning of methyltransferase domain of non-structural protein 5 (MTase NS5) we used a modified Gateway® pDEST™17 Vector (Life technologies) (insertion of an XhoI restriction site between His tag and attR1 site by site directed mutagenesis). .. SNAP-tag previously amplified from Plasmid Preparation:Article Title: High-Performance Binary Protein Interaction Screening in a Microfluidic Format Article Snippet: .. One microgram of Article Title: Protein-Protein Interaction Inhibition (2P2I)-Oriented Chemical Library Accelerates Hit Discovery. Article Snippet: For the cloning of methyltransferase domain of non-structural protein 5 (MTase NS5) we used a modified Gateway® pDEST™17 Vector (Life technologies) (insertion of an XhoI restriction site between His tag and attR1 site by site directed mutagenesis). .. SNAP-tag previously amplified from Article Title: Super-assembly of ER-phagy receptor Atg40 induces local ER remodeling at contacts with forming autophagosomal membranes Article Snippet: Plasmids for expression of fusion proteins for crystallization were constructed by inserting the genes encoding Atg40237–252 , human FAM134B450–468 , human SEC62361–376 with the T367D mutation, and human RTN3245–264 isoform e into upstream of the sequence encoding Atg8 K26P of pGEX6P-Atg8 (the K26P mutation was introduced for stabilizing Atg8) or upstream of the sequence encoding GABARAP of pGEX6P-GABARAP (with the F3S V4T mutations for enhancing crystallization). .. For phase-separation experiments, the mCherry and BNDL1 , sequences were inserted into upstream and downstream of the ATG8 gen e in pGEX6P-Atg8 K26P, respectively. pGEX-SNAP-40C was constructed by the removal of the HRV 3C protease recognition site and the insertion of the Polymerase Chain Reaction:Article Title: High-Performance Binary Protein Interaction Screening in a Microfluidic Format Article Snippet: .. One microgram of Article Title: Tet Proteins Regulate Neutrophil Granulation in Zebrafish through Demethylation of socs3b mRNA Article Snippet: Editing was assessed using a T7 endonuclease assay (NEB labs) per manufacturer instructions in pooled groups of 10 embryos. .. PCR primers for the Article Title: The bacterial virulence factors VopL and VopF nucleate actin from the pointed end Article Snippet: Plasmid construction VopL/F constructs were prepared by infusion (Takara Bio Inc.) after PCR amplification (iProof; Bio-Rad Laboratories) from plasmid DNA for VopL and from codon-optimized plasmid DNA for VopF (provided by E. Kerkhoff, University of Regensburg, Regensburg, Germany), with a 6xHis tag included in the reverse primer. .. PCR products were cloned into the Clone Assay:Article Title: The bacterial virulence factors VopL and VopF nucleate actin from the pointed end Article Snippet: Plasmid construction VopL/F constructs were prepared by infusion (Takara Bio Inc.) after PCR amplification (iProof; Bio-Rad Laboratories) from plasmid DNA for VopL and from codon-optimized plasmid DNA for VopF (provided by E. Kerkhoff, University of Regensburg, Regensburg, Germany), with a 6xHis tag included in the reverse primer. .. PCR products were cloned into the Countercurrent Chromatography:Article Title: Protein-Protein Interaction Inhibition (2P2I)-Oriented Chemical Library Accelerates Hit Discovery. Article Snippet: For the cloning of methyltransferase domain of non-structural protein 5 (MTase NS5) we used a modified Gateway® pDEST™17 Vector (Life technologies) (insertion of an XhoI restriction site between His tag and attR1 site by site directed mutagenesis). .. SNAP-tag previously amplified from Ligation:Article Title: Protein-Protein Interaction Inhibition (2P2I)-Oriented Chemical Library Accelerates Hit Discovery. Article Snippet: For the cloning of methyltransferase domain of non-structural protein 5 (MTase NS5) we used a modified Gateway® pDEST™17 Vector (Life technologies) (insertion of an XhoI restriction site between His tag and attR1 site by site directed mutagenesis). .. SNAP-tag previously amplified from Expressing:Article Title: Two-color STED microscopy in living cells Article Snippet: Combining these approaches with an imaging scheme that alternates line by line between different excitation beams to acquire two STED images quasi-simultaneously, we report the first example of two-color STED microscopy in living cells. .. 2.1 Expression Constructs pSNAPf -tag(T7) and pCLIPf -tag(T7) were constructed by replacing the SNAP-26m coding region of pSNAP-tag( Construct:Article Title: Two-color STED microscopy in living cells Article Snippet: Combining these approaches with an imaging scheme that alternates line by line between different excitation beams to acquire two STED images quasi-simultaneously, we report the first example of two-color STED microscopy in living cells. .. 2.1 Expression Constructs pSNAPf -tag(T7) and pCLIPf -tag(T7) were constructed by replacing the SNAP-26m coding region of pSNAP-tag( Article Title: Super-assembly of ER-phagy receptor Atg40 induces local ER remodeling at contacts with forming autophagosomal membranes Article Snippet: Plasmids for expression of fusion proteins for crystallization were constructed by inserting the genes encoding Atg40237–252 , human FAM134B450–468 , human SEC62361–376 with the T367D mutation, and human RTN3245–264 isoform e into upstream of the sequence encoding Atg8 K26P of pGEX6P-Atg8 (the K26P mutation was introduced for stabilizing Atg8) or upstream of the sequence encoding GABARAP of pGEX6P-GABARAP (with the F3S V4T mutations for enhancing crystallization). .. For phase-separation experiments, the mCherry and BNDL1 , sequences were inserted into upstream and downstream of the ATG8 gen e in pGEX6P-Atg8 K26P, respectively. pGEX-SNAP-40C was constructed by the removal of the HRV 3C protease recognition site and the insertion of the Sequencing:Article Title: Super-assembly of ER-phagy receptor Atg40 induces local ER remodeling at contacts with forming autophagosomal membranes Article Snippet: Plasmids for expression of fusion proteins for crystallization were constructed by inserting the genes encoding Atg40237–252 , human FAM134B450–468 , human SEC62361–376 with the T367D mutation, and human RTN3245–264 isoform e into upstream of the sequence encoding Atg8 K26P of pGEX6P-Atg8 (the K26P mutation was introduced for stabilizing Atg8) or upstream of the sequence encoding GABARAP of pGEX6P-GABARAP (with the F3S V4T mutations for enhancing crystallization). .. For phase-separation experiments, the mCherry and BNDL1 , sequences were inserted into upstream and downstream of the ATG8 gen e in pGEX6P-Atg8 K26P, respectively. pGEX-SNAP-40C was constructed by the removal of the HRV 3C protease recognition site and the insertion of the |