pgluc basic2  (New England Biolabs)


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    Structured Review

    New England Biolabs pgluc basic2
    Pgluc Basic2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 89/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgluc basic2/product/New England Biolabs
    Average 89 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    pgluc basic2 - by Bioz Stars, 2019-06
    89/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Human induced-pluripotent stem cell-derived hepatocyte-like cells as an in vitro model of human hepatitis B virus infection
    Article Snippet: Briefly, the genotype C HBV genome isolated from pHBV/C-AT was cloned into pHMCMV5 , yielding pHMCMV5-HBV. .. Next, the gaussia luciferase (gLuc) gene isolated from pGLuc-Basic2 (New England Biolabs, Hertfordshire, UK) was cloned into the downstream region of the CMV promoter in pHMCMV5-HBV, creating pHMCMV5-HBV-gLuc. pHMCMV5-gLuc was constructed by insertion of the gLuc gene into pHMCMV5. .. I-Ceu I/PI-Sce I-digested pHMCMV5-HBV-gLuc was ligated with I-Ceu I/PI-Sce I-digested pAdHM41-K7 , yielding pAdHM41-K7-HBV-gLuc. pAdHM41-K7 encodes a polylysine peptide (K7) in the C-terminal region of the Ad fiber knob. pAdHM41-K7-HBV-gLuc was digested with Pac I to release the recombinant viral genome, and was transfected into 293 cells plated on 60-mm dishes, resulting in AdK7-HBV-gLuc.

    Article Title: Toll-Like Receptor 9 Activation Rescues Impaired Antibody Response in Needle-free Intradermal DNA Vaccination
    Article Snippet: The HIV-1 tat gene was cloned in the pcDNA3.1 vector backbone (Cat #V920-20, Invitrogen, USA). .. The firefly and Gaussia luciferase genes were amplified using PCR from the commercial vectors pGL3 (E1751, Promega USA) and pGLuc-Basic2 (N8082, New England Biolabs, USA), respectively.

    Transfection:

    Article Title: Toll-Like Receptor 9 Activation Rescues Impaired Antibody Response in Needle-free Intradermal DNA Vaccination
    Article Snippet: The firefly and Gaussia luciferase genes were amplified using PCR from the commercial vectors pGL3 (E1751, Promega USA) and pGLuc-Basic2 (N8082, New England Biolabs, USA), respectively. .. All the amplified expression cassettes were cloned between EcoRI and XbaI (the restriction sites were highlighted in the primer sequences in italics) in respective vectors under the control of the CMV promoter.

    Luciferase:

    Article Title: Human induced-pluripotent stem cell-derived hepatocyte-like cells as an in vitro model of human hepatitis B virus infection
    Article Snippet: Briefly, the genotype C HBV genome isolated from pHBV/C-AT was cloned into pHMCMV5 , yielding pHMCMV5-HBV. .. Next, the gaussia luciferase (gLuc) gene isolated from pGLuc-Basic2 (New England Biolabs, Hertfordshire, UK) was cloned into the downstream region of the CMV promoter in pHMCMV5-HBV, creating pHMCMV5-HBV-gLuc. pHMCMV5-gLuc was constructed by insertion of the gLuc gene into pHMCMV5. .. I-Ceu I/PI-Sce I-digested pHMCMV5-HBV-gLuc was ligated with I-Ceu I/PI-Sce I-digested pAdHM41-K7 , yielding pAdHM41-K7-HBV-gLuc. pAdHM41-K7 encodes a polylysine peptide (K7) in the C-terminal region of the Ad fiber knob. pAdHM41-K7-HBV-gLuc was digested with Pac I to release the recombinant viral genome, and was transfected into 293 cells plated on 60-mm dishes, resulting in AdK7-HBV-gLuc.

    Article Title: Toll-Like Receptor 9 Activation Rescues Impaired Antibody Response in Needle-free Intradermal DNA Vaccination
    Article Snippet: The codon-optimized sequences were chemically synthesized by the commercial vendor ShineGene Molecular Biotech Inc, China. .. The firefly and Gaussia luciferase genes were amplified using PCR from the commercial vectors pGL3 (E1751, Promega USA) and pGLuc-Basic2 (N8082, New England Biolabs, USA), respectively. .. The firefly luc gene was amplified using the primer pair N2392 5′-CTCTGAATTC GCCGCCGCCATGGAAGACGCCAAAAACATAAAG-3′ and N2393 5′-GGGCCCTCTAGA TTACACGGCGATCTTTCCGC-3′ using the amplification conditions 9430” -6030” -7290” for 15 cycles.

    In Vitro:

    Article Title: Human induced-pluripotent stem cell-derived hepatocyte-like cells as an in vitro model of human hepatitis B virus infection
    Article Snippet: A fiber-modified adenovirus (Ad) vector containing the HBV genome was prepared by an improved in vitro ligation method . .. Next, the gaussia luciferase (gLuc) gene isolated from pGLuc-Basic2 (New England Biolabs, Hertfordshire, UK) was cloned into the downstream region of the CMV promoter in pHMCMV5-HBV, creating pHMCMV5-HBV-gLuc. pHMCMV5-gLuc was constructed by insertion of the gLuc gene into pHMCMV5.

    Fluorescence:

    Article Title: Human induced-pluripotent stem cell-derived hepatocyte-like cells as an in vitro model of human hepatitis B virus infection
    Article Snippet: Next, the gaussia luciferase (gLuc) gene isolated from pGLuc-Basic2 (New England Biolabs, Hertfordshire, UK) was cloned into the downstream region of the CMV promoter in pHMCMV5-HBV, creating pHMCMV5-HBV-gLuc. pHMCMV5-gLuc was constructed by insertion of the gLuc gene into pHMCMV5. .. AdK7-gLuc, which is a control Ad vector expressing gLuc, was similarly prepared using pHMCMV5-gLuc and pAdHM41-K7.

    Ligation:

    Article Title: Human induced-pluripotent stem cell-derived hepatocyte-like cells as an in vitro model of human hepatitis B virus infection
    Article Snippet: A fiber-modified adenovirus (Ad) vector containing the HBV genome was prepared by an improved in vitro ligation method . .. Next, the gaussia luciferase (gLuc) gene isolated from pGLuc-Basic2 (New England Biolabs, Hertfordshire, UK) was cloned into the downstream region of the CMV promoter in pHMCMV5-HBV, creating pHMCMV5-HBV-gLuc. pHMCMV5-gLuc was constructed by insertion of the gLuc gene into pHMCMV5.

    Isolation:

    Article Title: Human induced-pluripotent stem cell-derived hepatocyte-like cells as an in vitro model of human hepatitis B virus infection
    Article Snippet: Briefly, the genotype C HBV genome isolated from pHBV/C-AT was cloned into pHMCMV5 , yielding pHMCMV5-HBV. .. Next, the gaussia luciferase (gLuc) gene isolated from pGLuc-Basic2 (New England Biolabs, Hertfordshire, UK) was cloned into the downstream region of the CMV promoter in pHMCMV5-HBV, creating pHMCMV5-HBV-gLuc. pHMCMV5-gLuc was constructed by insertion of the gLuc gene into pHMCMV5. .. I-Ceu I/PI-Sce I-digested pHMCMV5-HBV-gLuc was ligated with I-Ceu I/PI-Sce I-digested pAdHM41-K7 , yielding pAdHM41-K7-HBV-gLuc. pAdHM41-K7 encodes a polylysine peptide (K7) in the C-terminal region of the Ad fiber knob. pAdHM41-K7-HBV-gLuc was digested with Pac I to release the recombinant viral genome, and was transfected into 293 cells plated on 60-mm dishes, resulting in AdK7-HBV-gLuc.

    Synthesized:

    Article Title: Toll-Like Receptor 9 Activation Rescues Impaired Antibody Response in Needle-free Intradermal DNA Vaccination
    Article Snippet: The codon-optimized sequences were chemically synthesized by the commercial vendor ShineGene Molecular Biotech Inc, China. .. The firefly and Gaussia luciferase genes were amplified using PCR from the commercial vectors pGL3 (E1751, Promega USA) and pGLuc-Basic2 (N8082, New England Biolabs, USA), respectively.

    Construct:

    Article Title: Human induced-pluripotent stem cell-derived hepatocyte-like cells as an in vitro model of human hepatitis B virus infection
    Article Snippet: Briefly, the genotype C HBV genome isolated from pHBV/C-AT was cloned into pHMCMV5 , yielding pHMCMV5-HBV. .. Next, the gaussia luciferase (gLuc) gene isolated from pGLuc-Basic2 (New England Biolabs, Hertfordshire, UK) was cloned into the downstream region of the CMV promoter in pHMCMV5-HBV, creating pHMCMV5-HBV-gLuc. pHMCMV5-gLuc was constructed by insertion of the gLuc gene into pHMCMV5. .. I-Ceu I/PI-Sce I-digested pHMCMV5-HBV-gLuc was ligated with I-Ceu I/PI-Sce I-digested pAdHM41-K7 , yielding pAdHM41-K7-HBV-gLuc. pAdHM41-K7 encodes a polylysine peptide (K7) in the C-terminal region of the Ad fiber knob. pAdHM41-K7-HBV-gLuc was digested with Pac I to release the recombinant viral genome, and was transfected into 293 cells plated on 60-mm dishes, resulting in AdK7-HBV-gLuc.

    Polymerase Chain Reaction:

    Article Title: Toll-Like Receptor 9 Activation Rescues Impaired Antibody Response in Needle-free Intradermal DNA Vaccination
    Article Snippet: The codon-optimized sequences were chemically synthesized by the commercial vendor ShineGene Molecular Biotech Inc, China. .. The firefly and Gaussia luciferase genes were amplified using PCR from the commercial vectors pGL3 (E1751, Promega USA) and pGLuc-Basic2 (N8082, New England Biolabs, USA), respectively. .. The firefly luc gene was amplified using the primer pair N2392 5′-CTCTGAATTC GCCGCCGCCATGGAAGACGCCAAAAACATAAAG-3′ and N2393 5′-GGGCCCTCTAGA TTACACGGCGATCTTTCCGC-3′ using the amplification conditions 9430” -6030” -7290” for 15 cycles.

    Generated:

    Article Title: Toll-Like Receptor 9 Activation Rescues Impaired Antibody Response in Needle-free Intradermal DNA Vaccination
    Article Snippet: The sequences of the HIV-1 gag and tat were of the consensus subtype C gag and tat , generated from 150 sequences representative of different countries downloaded from the HIV sequence database . .. The firefly and Gaussia luciferase genes were amplified using PCR from the commercial vectors pGL3 (E1751, Promega USA) and pGLuc-Basic2 (N8082, New England Biolabs, USA), respectively.

    Amplification:

    Article Title: Toll-Like Receptor 9 Activation Rescues Impaired Antibody Response in Needle-free Intradermal DNA Vaccination
    Article Snippet: The codon-optimized sequences were chemically synthesized by the commercial vendor ShineGene Molecular Biotech Inc, China. .. The firefly and Gaussia luciferase genes were amplified using PCR from the commercial vectors pGL3 (E1751, Promega USA) and pGLuc-Basic2 (N8082, New England Biolabs, USA), respectively. .. The firefly luc gene was amplified using the primer pair N2392 5′-CTCTGAATTC GCCGCCGCCATGGAAGACGCCAAAAACATAAAG-3′ and N2393 5′-GGGCCCTCTAGA TTACACGGCGATCTTTCCGC-3′ using the amplification conditions 9430” -6030” -7290” for 15 cycles.

    Expressing:

    Article Title: Human induced-pluripotent stem cell-derived hepatocyte-like cells as an in vitro model of human hepatitis B virus infection
    Article Snippet: Next, the gaussia luciferase (gLuc) gene isolated from pGLuc-Basic2 (New England Biolabs, Hertfordshire, UK) was cloned into the downstream region of the CMV promoter in pHMCMV5-HBV, creating pHMCMV5-HBV-gLuc. pHMCMV5-gLuc was constructed by insertion of the gLuc gene into pHMCMV5. .. I-Ceu I/PI-Sce I-digested pHMCMV5-HBV-gLuc was ligated with I-Ceu I/PI-Sce I-digested pAdHM41-K7 , yielding pAdHM41-K7-HBV-gLuc. pAdHM41-K7 encodes a polylysine peptide (K7) in the C-terminal region of the Ad fiber knob. pAdHM41-K7-HBV-gLuc was digested with Pac I to release the recombinant viral genome, and was transfected into 293 cells plated on 60-mm dishes, resulting in AdK7-HBV-gLuc.

    Article Title: Toll-Like Receptor 9 Activation Rescues Impaired Antibody Response in Needle-free Intradermal DNA Vaccination
    Article Snippet: Furthermore, the consensus subtype C gene sequences were codon-optimized for mammalian expression using the online codon optimization tool JCat ( http://www.jcat.de ). .. The firefly and Gaussia luciferase genes were amplified using PCR from the commercial vectors pGL3 (E1751, Promega USA) and pGLuc-Basic2 (N8082, New England Biolabs, USA), respectively.

    Sequencing:

    Article Title: Toll-Like Receptor 9 Activation Rescues Impaired Antibody Response in Needle-free Intradermal DNA Vaccination
    Article Snippet: The sequences of the HIV-1 gag and tat were of the consensus subtype C gag and tat , generated from 150 sequences representative of different countries downloaded from the HIV sequence database . .. The firefly and Gaussia luciferase genes were amplified using PCR from the commercial vectors pGL3 (E1751, Promega USA) and pGLuc-Basic2 (N8082, New England Biolabs, USA), respectively.

    Western Blot:

    Article Title: Toll-Like Receptor 9 Activation Rescues Impaired Antibody Response in Needle-free Intradermal DNA Vaccination
    Article Snippet: The firefly and Gaussia luciferase genes were amplified using PCR from the commercial vectors pGL3 (E1751, Promega USA) and pGLuc-Basic2 (N8082, New England Biolabs, USA), respectively. .. All the amplified expression cassettes were cloned between EcoRI and XbaI (the restriction sites were highlighted in the primer sequences in italics) in respective vectors under the control of the CMV promoter.

    Plasmid Preparation:

    Article Title: Human induced-pluripotent stem cell-derived hepatocyte-like cells as an in vitro model of human hepatitis B virus infection
    Article Snippet: Paragraph title: Preparation of a fiber-modified adenovirus (Ad) vector containing the HBV genome ... Next, the gaussia luciferase (gLuc) gene isolated from pGLuc-Basic2 (New England Biolabs, Hertfordshire, UK) was cloned into the downstream region of the CMV promoter in pHMCMV5-HBV, creating pHMCMV5-HBV-gLuc. pHMCMV5-gLuc was constructed by insertion of the gLuc gene into pHMCMV5.

    Article Title: Toll-Like Receptor 9 Activation Rescues Impaired Antibody Response in Needle-free Intradermal DNA Vaccination
    Article Snippet: Paragraph title: Plasmid DNA vectors and reagents ... The firefly and Gaussia luciferase genes were amplified using PCR from the commercial vectors pGL3 (E1751, Promega USA) and pGLuc-Basic2 (N8082, New England Biolabs, USA), respectively.

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    pGLuc basic Vector 20 ug
      Buy from Supplier

    89
    New England Biolabs pgluc basic2
    Pgluc Basic2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 89/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgluc basic2/product/New England Biolabs
    Average 89 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    pgluc basic2 - by Bioz Stars, 2019-06
    89/100 stars
      Buy from Supplier

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