gaussia luciferase  (New England Biolabs)


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    Name:
    BioLux Gaussia Luciferase Assay Kit
    Description:
    BioLux Gaussia Luciferase Assay Kit 1 000 assays
    Catalog Number:
    E3300L
    Price:
    485
    Size:
    1 000 assays
    Category:
    Cellular Biology
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    Structured Review

    New England Biolabs gaussia luciferase
    BioLux Gaussia Luciferase Assay Kit
    BioLux Gaussia Luciferase Assay Kit 1 000 assays
    https://www.bioz.com/result/gaussia luciferase/product/New England Biolabs
    Average 99 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    gaussia luciferase - by Bioz Stars, 2019-07
    99/100 stars

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    Related Articles

    Transduction:

    Article Title: Entry Tropism of BK and Merkel Cell Polyomaviruses in Cell Culture
    Article Snippet: Paragraph title: Cell Transduction Experiments ... A BMG Labtech Polarstar Optima luminometer was used to inject 50 µl of Gaussia Luciferase Assay Kit substrate (NEB), and light emission (in relative light units, RLUs) was measured according to manufacturer instructions.

    Clone Assay:

    Article Title: An Enhancer Element Harboring Variants Associated with Systemic Lupus Erythematosus Engages the TNFAIP3 Promoter to Influence A20 Expression
    Article Snippet: We cloned 340 bp (non-risk) or 339 bp (risk) of DNA sequence surrounding the TT > A polymorphism into a minimal promoter luciferase plasmid, pGLuc-mini-TK (New England BioLab, Ipswich, MA). .. To assay enhancer activity, Gaussia luciferase was analyzed from the cell culture media using BioLux GLuc assay kit (New England BioLab).

    Article Title: Genetically tunable frustration controls allostery in an intrinsically disordered transcription factor
    Article Snippet: For the competitive transfection assay, 40 ng of pGluc-miniTK vector with four tandem half site GREs cloned in the promoter region, 40 ng of pCluc-miniTK2, 3 ng of expression vector for C3 isoform, and up to 16 ng of plasmid coding for one of the competitors were co-transfected into U-2 OS cells on 96-well plates. .. After 48 hr, Gaussia Luciferase activity and Cypridina Luciferase activity were measured with the BioLux Gaussia Luciferase Assay Kit (NEB) and the BioLux Cypridina Luciferase Assay Kit (NEB), respectively, on a TriStar LB 942 Multidetection Microplate Reader (Berthold Technologies GmbH & Co. KG, Bad Wildbad, Germany), according to the manufacturer’s protocols.

    Luciferase:

    Article Title: Entry Tropism of BK and Merkel Cell Polyomaviruses in Cell Culture
    Article Snippet: For measurement of Gaussia luciferase expression, approximately 72 hrs post-inoculation, the plate containing cells was agitated and 25 µl of conditioned culture supernatant was transferred to a white 96-well luminometry plate (Perkin Elmer). .. A BMG Labtech Polarstar Optima luminometer was used to inject 50 µl of Gaussia Luciferase Assay Kit substrate (NEB), and light emission (in relative light units, RLUs) was measured according to manufacturer instructions. .. The middle dose of virus on A549 cells typically resulted in 150,000–200,000 RLUs with a background of ∼500 RLUs.

    Article Title: Cell-to-Cell Transmission Can Overcome Multiple Donor and Target Cell Barriers Imposed on Cell-Free HIV
    Article Snippet: Antibody against HIV Gag (clone KC57) was purchased from Beckman Coulter. .. Luciferase activity was measured using the BioLux Gaussia Luciferase Assay Kit (New England Biolabs). .. Co-culture experiments were performed using HEK293 cells transiently transfected with the plasmids encoding full length HIV (molecular clone NL4-3) and HIV-inGLuc (at a ratio of 6∶1) using Fugene 6 Transfection Reagent (Roche) or XtremeGene9 (Roche).

    Article Title: Class A Scavenger Receptor 1 (MSR1) Restricts Hepatitis C Virus Replication by Mediating Toll-like Receptor 3 Recognition of Viral RNAs Produced in Neighboring Cells
    Article Snippet: Cells were infected at an m.o.i. of 1. .. GLuc activity in supernatants was measured by BioLux Gaussia Luciferase Assay Kit (New England Biolabs, Ipswich, MA) using a Synergy2 multi-mode microplate reader (BioTek, Winooski, VT). .. HJ3-5/5A-YFP is another derivative of HJ3-5 containing yellow fluorescent protein (YFP) coding sequence fused to NS5A sequence .

    Article Title: Establishment and Characterization of the Reversibly Immortalized Mouse Fetal Heart Progenitors
    Article Snippet: Gaussia luciferase possesses a natural secretory signal and upon expression is secreted into the cell medium. .. At the indicated time points medium from the treated cells was collected for Gaussia luciferase assays using the Gaussia Luciferase Assay Kit (New England Biolabs) as described - . .. Each assay condition was performed in triplicate.

    Article Title: In Vitro Screening for Compounds That Enhance Human L1 Mobilization
    Article Snippet: After an additional 3-day incubation, two 20-µl aliquots of the medium were sampled to measure GLuc and CLuc. .. Luminescence was measured with the Multilabel Counter 1420 ARVO using the BioLux Gaussia luciferase assay kit (New England BioLabs) and BioLux Cypridina luciferase assay kit (New England BioLabs). .. Four or six independent assays were performed in quadruplicate.

    Article Title: An Enhancer Element Harboring Variants Associated with Systemic Lupus Erythematosus Engages the TNFAIP3 Promoter to Influence A20 Expression
    Article Snippet: Twenty fours hours post transfection, cells were treated with 1 ug/ml LPS for 24 hours or 50 ng/ml PMA/500 ng/ml ionomycin for 48 hours. .. To assay enhancer activity, Gaussia luciferase was analyzed from the cell culture media using BioLux GLuc assay kit (New England BioLab). .. To measure transfection efficiency, cells were lysed and firefly luciferase activity was measured using the Luciferase Assay System (Promega, Madison, WI).

    Article Title: The P body protein LSm1 contributes to stimulation of hepatitis C virus translation, but not replication, by microRNA-122
    Article Snippet: A fraction of cells electroporated with Bi-Gluc-H77C(1a)/JFH1 RNA were plated in 24-well plates for luciferase assays. .. Ten microliters of cell supernatant from triplicate wells was harvested at 1, 2, 3, 4, 6 and 24 h time points in luciferase lysis buffer (NEB), and assayed with Gaussia luciferase assay reagent (NEB). .. Immunoprecipitation of Argonaute complexes was performed on electroporated cells cultured in 10-cm plates as described in , except that the monoclonal antibody 11A9 (Sigma SAB4200085) was used to immunoprecipitate Ago2.

    Article Title: SRA Regulates Adipogenesis by Modulating p38/JNK Phosphorylation and Stimulating Insulin Receptor Gene Expression and Downstream Signaling
    Article Snippet: Sequences of the qPCR primers are available upon request or have been previously described . .. 3T3-L1 preadipocytes were cotransfected with pIRP-GLuc or pGluc-Basic (100 ng) and either pSCT or pSCT-SRA, pSCT-SRAP or pSCT-SRAP silent mutant plasmids for 48 hr using Lipofectamine Plus Reagent (Invitrogen) in 24 well plates, and luciferase activity was measured using a BioLux Gaussia Luciferase Assay Kit (New England Biolabs, Inc.). .. Results are presented as the mean±SD.

    Article Title: Leukemia Inhibitory Factor Downregulates Human Papillomavirus-16 Oncogene Expression and Inhibits the Proliferation of Cervical Carcinoma Cells
    Article Snippet: Following incubation, 20 (micro)L of supernatant from each well was transferred to a new plate. .. Gaussia luciferase reagent (New England Biolabs) was added and the luminescence measured for 5 seconds. .. In order to directly measure the expression of E6 and E7 mRNA, we designed primer sets and probes specific to the E6 gene and the E7 gene; oligonucleotides were synthesized by Eurofins MWG Operon (Huntsville, AL).

    Article Title: Anti-Melanogenic Activity of Gagunin D, a Highly Oxygenated Diterpenoid from the Marine Sponge Phorbas sp., via Modulating Tyrosinase Expression and Degradation
    Article Snippet: At 40%–50% confluency, the cells were washed with DPBS, and the Gaussia luciferase reporter construct pMITF-Gluc, pTYR-Gluc, and the control firefly luciferase vector (pGL3-FL) were transfected using FuGENE® HD Transfection Reagent (Promega, Madison, WI, USA). .. After a 24 h incubation with GD, the cell lysates were prepared and the Gaussia and firefly luciferase activity were determined by the Gaussia luciferase assay kit (New England BioLabs, Ipswich, MA, USA) and luciferase reporter assay system (Promega, Madison, WI, USA), respectively, according to the manufacturers’ protocols, using a luminometer (MicroLumat Plus, Berthold Technologies, Dortmund, Germany). .. The reconstructed human skin model, Neoderm® -ME, was purchased from Tego Science (Seoul, Korea) and was analyzed according to the manufacturer’s instructions.

    Article Title: Reassortment compatibility between PB1, PB2, and HA genes of the two influenza B virus lineages in mammalian cells
    Article Snippet: To investigate the polymerase activity of viral RNP complexes (PB1, PB2, PA, and NP genes), we additionally constructed a Gaussia luciferase reporter plasmid (pPolI-GLuc) . .. The RNP complex gene and the pPolI-GLuc plasmids were transfected into 5 × 104 293T cells (ATCC, Manassas, VA, USA) using X-tremeGENE (Roche, Basel, Switzerland), and the cells were incubated at 37 °C for 24 h. After treating the cells using BioLux Gaussia Luciferase Assay Kit (New England Biolabs, Ipswich, MA, USA), luciferase activity was measured on a SpectraMax L plate reader (Molecular Devices, Sunnyvale, CA, USA). .. The cells transfected with empty plasmids were used as background luciferase expression.

    Article Title: Exogenous TNFR2 activation protects from acute GvHD via host T reg cell expansion
    Article Snippet: The next day, transfected cells were seeded into 24-well plates. .. After an additional day, the cells were incubated pairwise at 37°C for 2 h with increasing concentrations of GpL-TNC–mouse TNF and GpL-STAR2, and cell-bound luciferase activity was assayed using the Gaussia luciferase assay kit (New England Biolabs, Inc.) on a Lucy 2 luminometer (Anthos Labtec Instruments). .. Specific binding values were calculated for the two GpL fusion proteins by subtracting the values obtained for empty vector–transfected cells (nonspecific binding) from the corresponding values verified for the TNFR2 transfectants.

    Article Title: Genetically tunable frustration controls allostery in an intrinsically disordered transcription factor
    Article Snippet: For titration of the C3 isoform wild type and C3 C431Y & V435A & L436A mutant with the R domain-nuclear localization sequence-Flag construct, the method was the same as the competitive transfection assay described above, except up to 12 ng of the R domain-nuclear localization sequence-Flag construct plasmid was used in the titration. .. After 48 hr, Gaussia Luciferase activity and Cypridina Luciferase activity were measured with the BioLux Gaussia Luciferase Assay Kit (NEB) and the BioLux Cypridina Luciferase Assay Kit (NEB), respectively, on a TriStar LB 942 Multidetection Microplate Reader (Berthold Technologies GmbH & Co. KG, Bad Wildbad, Germany), according to the manufacturer’s protocols. .. In each experiment, the Gaussia luciferase activity (normalized by the Cypridina luciferase activity) was measured in triplicate and averaged.

    Article Title: KLF4-dependent perivascular cell plasticity mediates pre-metastatic niche formation and metastasis
    Article Snippet: 72 hours post-siRNA treatment, media was replaced with serum-free media containing recombinant proteins or tumor conditioned media. .. 16 hours after protein-containing treatment, media was removed and assayed for luciferase activity using the BioLux Gaussia Luciferase Assay Kit (New England BioLabs). .. Reporter plasmid activity is reported in luminescence as compared to empty plasmid control.

    Article Title: Engineering circular RNA for potent and stable translation in eukaryotic cells
    Article Snippet: To detect luminescence from Gaussia luciferase, 10–20 µL of tissue culture medium was transferred to a flat-bottomed white-walled plate (Corning). .. 25 µL of BioLux Gaussia Luciferase reagent including stabilizer (New England Biolabs) was added to each sample and luminescence was measured on an Infinite 200Pro Microplate Reader (Tecan) after 45 s. To detect luminescence from Firefly luciferase, 100 µL of Bright-Glo Luciferase reagent (Promega) was added to each well, mixed, and incubated for 5 min. 100 µL of the culture medium and luciferase reagent mix was then transferred to a flat-bottomed white-walled plate and luminescence was detected as described above. .. GFP fluorescence was detected 24 h post-transfection and images were taken using an EVOS FL cell imager (Invitrogen).

    Article Title: Altered expression and function of regulator of G-protein signaling-17 (RGS17) in hepatocellular carcinoma
    Article Snippet: IMPROM II™ transcription system, psiLentGene vector, GoTaq green master mix, DualGlo Luciferase Assay, and the cAMP-Glo kit were purchased from Promega (Madison, WI). .. The pGLuc plasmid (possessing Gaussian luciferase) and a BioLux Gaussia Luciferase Assay Kit were purchased from New England Biolabs (Ipswich, MA). .. Antibodies against SUMO-1, SUMO-1/ 3, β-actin and Gqα were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

    Article Title: The effect of glycosaminoglycan content on polyethylenimine-based gene delivery within three-dimensional collagen-GAG scaffolds
    Article Snippet: The results were normalized to relative light units measured from tenocyte seeded scaffolds that were treated with the pGL3 plasmid only with no PEI carrier. .. Luciferase expression was subsequently quantified over time (through 14 days) after cell seeding using the BioLux Gaussia Luciferase Assay Kit (New England BioLabs). .. Aliquots of media (50 μL) were taken at regular intervals (2, 5, 7, 14 days post transfection) and stored for analysis at either 4°C (less than 1 week storage) or −20°C (longer than 1 week storage).

    Reporter Assay:

    Article Title: Establishment and Characterization of the Reversibly Immortalized Mouse Fetal Heart Progenitors
    Article Snippet: Paragraph title: Transfection and Gaussia luciferase reporter assay ... At the indicated time points medium from the treated cells was collected for Gaussia luciferase assays using the Gaussia Luciferase Assay Kit (New England Biolabs) as described - .

    Article Title: Anti-Melanogenic Activity of Gagunin D, a Highly Oxygenated Diterpenoid from the Marine Sponge Phorbas sp., via Modulating Tyrosinase Expression and Degradation
    Article Snippet: At 40%–50% confluency, the cells were washed with DPBS, and the Gaussia luciferase reporter construct pMITF-Gluc, pTYR-Gluc, and the control firefly luciferase vector (pGL3-FL) were transfected using FuGENE® HD Transfection Reagent (Promega, Madison, WI, USA). .. After a 24 h incubation with GD, the cell lysates were prepared and the Gaussia and firefly luciferase activity were determined by the Gaussia luciferase assay kit (New England BioLabs, Ipswich, MA, USA) and luciferase reporter assay system (Promega, Madison, WI, USA), respectively, according to the manufacturers’ protocols, using a luminometer (MicroLumat Plus, Berthold Technologies, Dortmund, Germany). .. The reconstructed human skin model, Neoderm® -ME, was purchased from Tego Science (Seoul, Korea) and was analyzed according to the manufacturer’s instructions.

    Article Title: Genetically tunable frustration controls allostery in an intrinsically disordered transcription factor
    Article Snippet: Paragraph title: Transcriptional activity by dual luciferase reporter assay ... After 48 hr, Gaussia Luciferase activity and Cypridina Luciferase activity were measured with the BioLux Gaussia Luciferase Assay Kit (NEB) and the BioLux Cypridina Luciferase Assay Kit (NEB), respectively, on a TriStar LB 942 Multidetection Microplate Reader (Berthold Technologies GmbH & Co. KG, Bad Wildbad, Germany), according to the manufacturer’s protocols.

    Expressing:

    Article Title: Entry Tropism of BK and Merkel Cell Polyomaviruses in Cell Culture
    Article Snippet: For measurement of Gaussia luciferase expression, approximately 72 hrs post-inoculation, the plate containing cells was agitated and 25 µl of conditioned culture supernatant was transferred to a white 96-well luminometry plate (Perkin Elmer). .. A BMG Labtech Polarstar Optima luminometer was used to inject 50 µl of Gaussia Luciferase Assay Kit substrate (NEB), and light emission (in relative light units, RLUs) was measured according to manufacturer instructions.

    Article Title: Establishment and Characterization of the Reversibly Immortalized Mouse Fetal Heart Progenitors
    Article Snippet: Gaussia luciferase possesses a natural secretory signal and upon expression is secreted into the cell medium. .. At the indicated time points medium from the treated cells was collected for Gaussia luciferase assays using the Gaussia Luciferase Assay Kit (New England Biolabs) as described - .

    Article Title: Synthetic mammalian trigger-controlled bipartite transcription factors
    Article Snippet: Paragraph title: Quantification of reporter gene expression ... GLuc was quantified using the BioLux® GLuc assay (E3300S, New England Biolabs) and the EnVision 2104 multilabel reader (maximum emission 482 nm).

    Article Title: Exogenous TNFR2 activation protects from acute GvHD via host T reg cell expansion
    Article Snippet: To analyze the binding of wild-type mouse TNF and mouse STAR2 to mouse TNFR2, HEK293 cells were electroporated with an empty vector or a mouse TNFR2 expression plasmid. .. After an additional day, the cells were incubated pairwise at 37°C for 2 h with increasing concentrations of GpL-TNC–mouse TNF and GpL-STAR2, and cell-bound luciferase activity was assayed using the Gaussia luciferase assay kit (New England Biolabs, Inc.) on a Lucy 2 luminometer (Anthos Labtec Instruments).

    Article Title: Genetically tunable frustration controls allostery in an intrinsically disordered transcription factor
    Article Snippet: For the competitive transfection assay, 40 ng of pGluc-miniTK vector with four tandem half site GREs cloned in the promoter region, 40 ng of pCluc-miniTK2, 3 ng of expression vector for C3 isoform, and up to 16 ng of plasmid coding for one of the competitors were co-transfected into U-2 OS cells on 96-well plates. .. After 48 hr, Gaussia Luciferase activity and Cypridina Luciferase activity were measured with the BioLux Gaussia Luciferase Assay Kit (NEB) and the BioLux Cypridina Luciferase Assay Kit (NEB), respectively, on a TriStar LB 942 Multidetection Microplate Reader (Berthold Technologies GmbH & Co. KG, Bad Wildbad, Germany), according to the manufacturer’s protocols.

    Article Title: Engineering circular RNA for potent and stable translation in eukaryotic cells
    Article Snippet: Paragraph title: Protein expression analysis ... 25 µL of BioLux Gaussia Luciferase reagent including stabilizer (New England Biolabs) was added to each sample and luminescence was measured on an Infinite 200Pro Microplate Reader (Tecan) after 45 s. To detect luminescence from Firefly luciferase, 100 µL of Bright-Glo Luciferase reagent (Promega) was added to each well, mixed, and incubated for 5 min. 100 µL of the culture medium and luciferase reagent mix was then transferred to a flat-bottomed white-walled plate and luminescence was detected as described above.

    Article Title: The effect of glycosaminoglycan content on polyethylenimine-based gene delivery within three-dimensional collagen-GAG scaffolds
    Article Snippet: The results were normalized to relative light units measured from tenocyte seeded scaffolds that were treated with the pGL3 plasmid only with no PEI carrier. .. Luciferase expression was subsequently quantified over time (through 14 days) after cell seeding using the BioLux Gaussia Luciferase Assay Kit (New England BioLabs). .. Aliquots of media (50 μL) were taken at regular intervals (2, 5, 7, 14 days post transfection) and stored for analysis at either 4°C (less than 1 week storage) or −20°C (longer than 1 week storage).

    Construct:

    Article Title: Anti-Melanogenic Activity of Gagunin D, a Highly Oxygenated Diterpenoid from the Marine Sponge Phorbas sp., via Modulating Tyrosinase Expression and Degradation
    Article Snippet: At 40%–50% confluency, the cells were washed with DPBS, and the Gaussia luciferase reporter construct pMITF-Gluc, pTYR-Gluc, and the control firefly luciferase vector (pGL3-FL) were transfected using FuGENE® HD Transfection Reagent (Promega, Madison, WI, USA). .. After a 24 h incubation with GD, the cell lysates were prepared and the Gaussia and firefly luciferase activity were determined by the Gaussia luciferase assay kit (New England BioLabs, Ipswich, MA, USA) and luciferase reporter assay system (Promega, Madison, WI, USA), respectively, according to the manufacturers’ protocols, using a luminometer (MicroLumat Plus, Berthold Technologies, Dortmund, Germany).

    Article Title: Reassortment compatibility between PB1, PB2, and HA genes of the two influenza B virus lineages in mammalian cells
    Article Snippet: To investigate the polymerase activity of viral RNP complexes (PB1, PB2, PA, and NP genes), we additionally constructed a Gaussia luciferase reporter plasmid (pPolI-GLuc) . .. The RNP complex gene and the pPolI-GLuc plasmids were transfected into 5 × 104 293T cells (ATCC, Manassas, VA, USA) using X-tremeGENE (Roche, Basel, Switzerland), and the cells were incubated at 37 °C for 24 h. After treating the cells using BioLux Gaussia Luciferase Assay Kit (New England Biolabs, Ipswich, MA, USA), luciferase activity was measured on a SpectraMax L plate reader (Molecular Devices, Sunnyvale, CA, USA).

    Article Title: Genetically tunable frustration controls allostery in an intrinsically disordered transcription factor
    Article Snippet: For titration of the C3 isoform wild type and C3 C431Y & V435A & L436A mutant with the R domain-nuclear localization sequence-Flag construct, the method was the same as the competitive transfection assay described above, except up to 12 ng of the R domain-nuclear localization sequence-Flag construct plasmid was used in the titration. .. After 48 hr, Gaussia Luciferase activity and Cypridina Luciferase activity were measured with the BioLux Gaussia Luciferase Assay Kit (NEB) and the BioLux Cypridina Luciferase Assay Kit (NEB), respectively, on a TriStar LB 942 Multidetection Microplate Reader (Berthold Technologies GmbH & Co. KG, Bad Wildbad, Germany), according to the manufacturer’s protocols.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Exogenous TNFR2 activation protects from acute GvHD via host T reg cell expansion
    Article Snippet: After an additional day, the cells were incubated pairwise at 37°C for 2 h with increasing concentrations of GpL-TNC–mouse TNF and GpL-STAR2, and cell-bound luciferase activity was assayed using the Gaussia luciferase assay kit (New England Biolabs, Inc.) on a Lucy 2 luminometer (Anthos Labtec Instruments). .. Specific binding values were calculated for the two GpL fusion proteins by subtracting the values obtained for empty vector–transfected cells (nonspecific binding) from the corresponding values verified for the TNFR2 transfectants.

    Sandwich ELISA:

    Article Title: Engineering circular RNA for potent and stable translation in eukaryotic cells
    Article Snippet: 25 µL of BioLux Gaussia Luciferase reagent including stabilizer (New England Biolabs) was added to each sample and luminescence was measured on an Infinite 200Pro Microplate Reader (Tecan) after 45 s. To detect luminescence from Firefly luciferase, 100 µL of Bright-Glo Luciferase reagent (Promega) was added to each well, mixed, and incubated for 5 min. 100 µL of the culture medium and luciferase reagent mix was then transferred to a flat-bottomed white-walled plate and luminescence was detected as described above. .. GFP fluorescence was detected 24 h post-transfection and images were taken using an EVOS FL cell imager (Invitrogen).

    Incubation:

    Article Title: Entry Tropism of BK and Merkel Cell Polyomaviruses in Cell Culture
    Article Snippet: To measure viral transduction of the GFP gene, approximately 72 hrs post-inoculation, adherent cells were incubated with trypsin to detach them from the plate and transferred to an untreated 96 well plate and suspended in wash medium (WM; DPBS with 1% FBS, antibiotic-antimycotic, and 10 mM HEPES, pH 8). .. A BMG Labtech Polarstar Optima luminometer was used to inject 50 µl of Gaussia Luciferase Assay Kit substrate (NEB), and light emission (in relative light units, RLUs) was measured according to manufacturer instructions.

    Article Title: In Vitro Screening for Compounds That Enhance Human L1 Mobilization
    Article Snippet: After an additional 3-day incubation, two 20-µl aliquots of the medium were sampled to measure GLuc and CLuc. .. Luminescence was measured with the Multilabel Counter 1420 ARVO using the BioLux Gaussia luciferase assay kit (New England BioLabs) and BioLux Cypridina luciferase assay kit (New England BioLabs).

    Article Title: Leukemia Inhibitory Factor Downregulates Human Papillomavirus-16 Oncogene Expression and Inhibits the Proliferation of Cervical Carcinoma Cells
    Article Snippet: Gaussia luciferase reagent (New England Biolabs) was added and the luminescence measured for 5 seconds. .. Gaussia luciferase reagent (New England Biolabs) was added and the luminescence measured for 5 seconds.

    Article Title: Anti-Melanogenic Activity of Gagunin D, a Highly Oxygenated Diterpenoid from the Marine Sponge Phorbas sp., via Modulating Tyrosinase Expression and Degradation
    Article Snippet: At 40%–50% confluency, the cells were washed with DPBS, and the Gaussia luciferase reporter construct pMITF-Gluc, pTYR-Gluc, and the control firefly luciferase vector (pGL3-FL) were transfected using FuGENE® HD Transfection Reagent (Promega, Madison, WI, USA). .. After a 24 h incubation with GD, the cell lysates were prepared and the Gaussia and firefly luciferase activity were determined by the Gaussia luciferase assay kit (New England BioLabs, Ipswich, MA, USA) and luciferase reporter assay system (Promega, Madison, WI, USA), respectively, according to the manufacturers’ protocols, using a luminometer (MicroLumat Plus, Berthold Technologies, Dortmund, Germany). .. The reconstructed human skin model, Neoderm® -ME, was purchased from Tego Science (Seoul, Korea) and was analyzed according to the manufacturer’s instructions.

    Article Title: Reassortment compatibility between PB1, PB2, and HA genes of the two influenza B virus lineages in mammalian cells
    Article Snippet: To investigate the polymerase activity of viral RNP complexes (PB1, PB2, PA, and NP genes), we additionally constructed a Gaussia luciferase reporter plasmid (pPolI-GLuc) . .. The RNP complex gene and the pPolI-GLuc plasmids were transfected into 5 × 104 293T cells (ATCC, Manassas, VA, USA) using X-tremeGENE (Roche, Basel, Switzerland), and the cells were incubated at 37 °C for 24 h. After treating the cells using BioLux Gaussia Luciferase Assay Kit (New England Biolabs, Ipswich, MA, USA), luciferase activity was measured on a SpectraMax L plate reader (Molecular Devices, Sunnyvale, CA, USA). .. The cells transfected with empty plasmids were used as background luciferase expression.

    Article Title: Exogenous TNFR2 activation protects from acute GvHD via host T reg cell expansion
    Article Snippet: The next day, transfected cells were seeded into 24-well plates. .. After an additional day, the cells were incubated pairwise at 37°C for 2 h with increasing concentrations of GpL-TNC–mouse TNF and GpL-STAR2, and cell-bound luciferase activity was assayed using the Gaussia luciferase assay kit (New England Biolabs, Inc.) on a Lucy 2 luminometer (Anthos Labtec Instruments). .. Specific binding values were calculated for the two GpL fusion proteins by subtracting the values obtained for empty vector–transfected cells (nonspecific binding) from the corresponding values verified for the TNFR2 transfectants.

    Article Title: Engineering circular RNA for potent and stable translation in eukaryotic cells
    Article Snippet: To detect luminescence from Gaussia luciferase, 10–20 µL of tissue culture medium was transferred to a flat-bottomed white-walled plate (Corning). .. 25 µL of BioLux Gaussia Luciferase reagent including stabilizer (New England Biolabs) was added to each sample and luminescence was measured on an Infinite 200Pro Microplate Reader (Tecan) after 45 s. To detect luminescence from Firefly luciferase, 100 µL of Bright-Glo Luciferase reagent (Promega) was added to each well, mixed, and incubated for 5 min. 100 µL of the culture medium and luciferase reagent mix was then transferred to a flat-bottomed white-walled plate and luminescence was detected as described above. .. GFP fluorescence was detected 24 h post-transfection and images were taken using an EVOS FL cell imager (Invitrogen).

    Proliferation Assay:

    Article Title: Altered expression and function of regulator of G-protein signaling-17 (RGS17) in hepatocellular carcinoma
    Article Snippet: The MTT proliferation assay and pcDNA3.1 were purchased from Invitrogen (Carlsbad, CA). .. The pGLuc plasmid (possessing Gaussian luciferase) and a BioLux Gaussia Luciferase Assay Kit were purchased from New England Biolabs (Ipswich, MA).

    Cell Culture:

    Article Title: Establishment and Characterization of the Reversibly Immortalized Mouse Fetal Heart Progenitors
    Article Snippet: Exponentially growing cells were seeded in 25cm2 cell culture flasks and transfected with 2μg per flask of pMyHC-GLuc using Lipofectamine (Invitrogen). .. At the indicated time points medium from the treated cells was collected for Gaussia luciferase assays using the Gaussia Luciferase Assay Kit (New England Biolabs) as described - .

    Article Title: An Enhancer Element Harboring Variants Associated with Systemic Lupus Erythematosus Engages the TNFAIP3 Promoter to Influence A20 Expression
    Article Snippet: Twenty fours hours post transfection, cells were treated with 1 ug/ml LPS for 24 hours or 50 ng/ml PMA/500 ng/ml ionomycin for 48 hours. .. To assay enhancer activity, Gaussia luciferase was analyzed from the cell culture media using BioLux GLuc assay kit (New England BioLab). .. To measure transfection efficiency, cells were lysed and firefly luciferase activity was measured using the Luciferase Assay System (Promega, Madison, WI).

    Article Title: The P body protein LSm1 contributes to stimulation of hepatitis C virus translation, but not replication, by microRNA-122
    Article Snippet: Paragraph title: Cell culture and transfection ... Ten microliters of cell supernatant from triplicate wells was harvested at 1, 2, 3, 4, 6 and 24 h time points in luciferase lysis buffer (NEB), and assayed with Gaussia luciferase assay reagent (NEB).

    Article Title: Engineering circular RNA for potent and stable translation in eukaryotic cells
    Article Snippet: 25 µL of BioLux Gaussia Luciferase reagent including stabilizer (New England Biolabs) was added to each sample and luminescence was measured on an Infinite 200Pro Microplate Reader (Tecan) after 45 s. To detect luminescence from Firefly luciferase, 100 µL of Bright-Glo Luciferase reagent (Promega) was added to each well, mixed, and incubated for 5 min. 100 µL of the culture medium and luciferase reagent mix was then transferred to a flat-bottomed white-walled plate and luminescence was detected as described above. .. GFP fluorescence was detected 24 h post-transfection and images were taken using an EVOS FL cell imager (Invitrogen).

    Mass Spectrometry:

    Article Title: The P body protein LSm1 contributes to stimulation of hepatitis C virus translation, but not replication, by microRNA-122
    Article Snippet: Cells (4 × 105 ) were resuspended in 10 μl of buffer R and mixed with 1 μg of wild-type or mutant H77ΔE1/p7, Bi-Gluc-H77C(1a)/JFH1, FL-J6/JFH1 or 5′LUC3′ RNA, and 20 pmoles 122-2′Ome or pre-miR-122 where included, before electroporation with a single pulse at 1300 V for 30 ms. For immunoprecipitation experiments, three electroporations were pooled and plated on a 10-cm plate. .. Ten microliters of cell supernatant from triplicate wells was harvested at 1, 2, 3, 4, 6 and 24 h time points in luciferase lysis buffer (NEB), and assayed with Gaussia luciferase assay reagent (NEB).

    Western Blot:

    Article Title: KLF4-dependent perivascular cell plasticity mediates pre-metastatic niche formation and metastasis
    Article Snippet: 16 hours after protein-containing treatment, media was removed and assayed for luciferase activity using the BioLux Gaussia Luciferase Assay Kit (New England BioLabs). .. 16 hours after protein-containing treatment, media was removed and assayed for luciferase activity using the BioLux Gaussia Luciferase Assay Kit (New England BioLabs).

    Recombinase Polymerase Amplification:

    Article Title: Cell-to-Cell Transmission Can Overcome Multiple Donor and Target Cell Barriers Imposed on Cell-Free HIV
    Article Snippet: Anti-CD4 antibody (clone RPA-T4) was purchased from eBiosciences. .. Luciferase activity was measured using the BioLux Gaussia Luciferase Assay Kit (New England Biolabs).

    Electroporation:

    Article Title: The P body protein LSm1 contributes to stimulation of hepatitis C virus translation, but not replication, by microRNA-122
    Article Snippet: Cells electroporated with HCV RNA were plated in six-well plates and total RNA extracted at 6 and 24 h after electroporation using TRI reagent. .. Ten microliters of cell supernatant from triplicate wells was harvested at 1, 2, 3, 4, 6 and 24 h time points in luciferase lysis buffer (NEB), and assayed with Gaussia luciferase assay reagent (NEB).

    Flow Cytometry:

    Article Title: Entry Tropism of BK and Merkel Cell Polyomaviruses in Cell Culture
    Article Snippet: Cells were then analyzed by flow cytometery for GFP reporter gene expression in a FACS Canto II with HTS (BD Biosciences). .. A BMG Labtech Polarstar Optima luminometer was used to inject 50 µl of Gaussia Luciferase Assay Kit substrate (NEB), and light emission (in relative light units, RLUs) was measured according to manufacturer instructions.

    Article Title: Exogenous TNFR2 activation protects from acute GvHD via host T reg cell expansion
    Article Snippet: After an additional day, the cells were incubated pairwise at 37°C for 2 h with increasing concentrations of GpL-TNC–mouse TNF and GpL-STAR2, and cell-bound luciferase activity was assayed using the Gaussia luciferase assay kit (New England Biolabs, Inc.) on a Lucy 2 luminometer (Anthos Labtec Instruments). .. Specific binding values were calculated for the two GpL fusion proteins by subtracting the values obtained for empty vector–transfected cells (nonspecific binding) from the corresponding values verified for the TNFR2 transfectants.

    Immunoprecipitation:

    Article Title: The P body protein LSm1 contributes to stimulation of hepatitis C virus translation, but not replication, by microRNA-122
    Article Snippet: Cells (4 × 105 ) were resuspended in 10 μl of buffer R and mixed with 1 μg of wild-type or mutant H77ΔE1/p7, Bi-Gluc-H77C(1a)/JFH1, FL-J6/JFH1 or 5′LUC3′ RNA, and 20 pmoles 122-2′Ome or pre-miR-122 where included, before electroporation with a single pulse at 1300 V for 30 ms. For immunoprecipitation experiments, three electroporations were pooled and plated on a 10-cm plate. .. Ten microliters of cell supernatant from triplicate wells was harvested at 1, 2, 3, 4, 6 and 24 h time points in luciferase lysis buffer (NEB), and assayed with Gaussia luciferase assay reagent (NEB).

    Infection:

    Article Title: Class A Scavenger Receptor 1 (MSR1) Restricts Hepatitis C Virus Replication by Mediating Toll-like Receptor 3 Recognition of Viral RNAs Produced in Neighboring Cells
    Article Snippet: Cells were infected at an m.o.i. of 1. .. GLuc activity in supernatants was measured by BioLux Gaussia Luciferase Assay Kit (New England Biolabs, Ipswich, MA) using a Synergy2 multi-mode microplate reader (BioTek, Winooski, VT).

    Imaging:

    Article Title: KLF4-dependent perivascular cell plasticity mediates pre-metastatic niche formation and metastasis
    Article Snippet: 16 hours after protein-containing treatment, media was removed and assayed for luciferase activity using the BioLux Gaussia Luciferase Assay Kit (New England BioLabs). .. 16 hours after protein-containing treatment, media was removed and assayed for luciferase activity using the BioLux Gaussia Luciferase Assay Kit (New England BioLabs).

    Sequencing:

    Article Title: Class A Scavenger Receptor 1 (MSR1) Restricts Hepatitis C Virus Replication by Mediating Toll-like Receptor 3 Recognition of Viral RNAs Produced in Neighboring Cells
    Article Snippet: HJ3-5/GLuc2A is a derivative of HJ3-5 containing the Gaussia princeps luciferase (GLuc) coding sequence fused to the foot-and-mouth disease virus (FMDV) 2A sequence and inserted between p7 and NS2 of HJ3-5 virus . .. GLuc activity in supernatants was measured by BioLux Gaussia Luciferase Assay Kit (New England Biolabs, Ipswich, MA) using a Synergy2 multi-mode microplate reader (BioTek, Winooski, VT).

    Article Title: An Enhancer Element Harboring Variants Associated with Systemic Lupus Erythematosus Engages the TNFAIP3 Promoter to Influence A20 Expression
    Article Snippet: We cloned 340 bp (non-risk) or 339 bp (risk) of DNA sequence surrounding the TT > A polymorphism into a minimal promoter luciferase plasmid, pGLuc-mini-TK (New England BioLab, Ipswich, MA). .. To assay enhancer activity, Gaussia luciferase was analyzed from the cell culture media using BioLux GLuc assay kit (New England BioLab).

    Article Title: Genetically tunable frustration controls allostery in an intrinsically disordered transcription factor
    Article Snippet: For titration of the C3 isoform wild type and C3 C431Y & V435A & L436A mutant with the R domain-nuclear localization sequence-Flag construct, the method was the same as the competitive transfection assay described above, except up to 12 ng of the R domain-nuclear localization sequence-Flag construct plasmid was used in the titration. .. After 48 hr, Gaussia Luciferase activity and Cypridina Luciferase activity were measured with the BioLux Gaussia Luciferase Assay Kit (NEB) and the BioLux Cypridina Luciferase Assay Kit (NEB), respectively, on a TriStar LB 942 Multidetection Microplate Reader (Berthold Technologies GmbH & Co. KG, Bad Wildbad, Germany), according to the manufacturer’s protocols.

    Binding Assay:

    Article Title: Exogenous TNFR2 activation protects from acute GvHD via host T reg cell expansion
    Article Snippet: Paragraph title: Binding studies ... After an additional day, the cells were incubated pairwise at 37°C for 2 h with increasing concentrations of GpL-TNC–mouse TNF and GpL-STAR2, and cell-bound luciferase activity was assayed using the Gaussia luciferase assay kit (New England Biolabs, Inc.) on a Lucy 2 luminometer (Anthos Labtec Instruments).

    Reporter Gene Assay:

    Article Title: SRA Regulates Adipogenesis by Modulating p38/JNK Phosphorylation and Stimulating Insulin Receptor Gene Expression and Downstream Signaling
    Article Snippet: Paragraph title: Luciferase Reporter Gene Assay ... 3T3-L1 preadipocytes were cotransfected with pIRP-GLuc or pGluc-Basic (100 ng) and either pSCT or pSCT-SRA, pSCT-SRAP or pSCT-SRAP silent mutant plasmids for 48 hr using Lipofectamine Plus Reagent (Invitrogen) in 24 well plates, and luciferase activity was measured using a BioLux Gaussia Luciferase Assay Kit (New England Biolabs, Inc.).

    MTT Assay:

    Article Title: Altered expression and function of regulator of G-protein signaling-17 (RGS17) in hepatocellular carcinoma
    Article Snippet: The MTT proliferation assay and pcDNA3.1 were purchased from Invitrogen (Carlsbad, CA). .. The pGLuc plasmid (possessing Gaussian luciferase) and a BioLux Gaussia Luciferase Assay Kit were purchased from New England Biolabs (Ipswich, MA).

    Mutagenesis:

    Article Title: The P body protein LSm1 contributes to stimulation of hepatitis C virus translation, but not replication, by microRNA-122
    Article Snippet: Cells (4 × 105 ) were resuspended in 10 μl of buffer R and mixed with 1 μg of wild-type or mutant H77ΔE1/p7, Bi-Gluc-H77C(1a)/JFH1, FL-J6/JFH1 or 5′LUC3′ RNA, and 20 pmoles 122-2′Ome or pre-miR-122 where included, before electroporation with a single pulse at 1300 V for 30 ms. For immunoprecipitation experiments, three electroporations were pooled and plated on a 10-cm plate. .. Ten microliters of cell supernatant from triplicate wells was harvested at 1, 2, 3, 4, 6 and 24 h time points in luciferase lysis buffer (NEB), and assayed with Gaussia luciferase assay reagent (NEB).

    Article Title: SRA Regulates Adipogenesis by Modulating p38/JNK Phosphorylation and Stimulating Insulin Receptor Gene Expression and Downstream Signaling
    Article Snippet: Sequences of the qPCR primers are available upon request or have been previously described . .. 3T3-L1 preadipocytes were cotransfected with pIRP-GLuc or pGluc-Basic (100 ng) and either pSCT or pSCT-SRA, pSCT-SRAP or pSCT-SRAP silent mutant plasmids for 48 hr using Lipofectamine Plus Reagent (Invitrogen) in 24 well plates, and luciferase activity was measured using a BioLux Gaussia Luciferase Assay Kit (New England Biolabs, Inc.). .. Results are presented as the mean±SD.

    Article Title: Genetically tunable frustration controls allostery in an intrinsically disordered transcription factor
    Article Snippet: For titration of the C3 isoform wild type and C3 C431Y & V435A & L436A mutant with the R domain-nuclear localization sequence-Flag construct, the method was the same as the competitive transfection assay described above, except up to 12 ng of the R domain-nuclear localization sequence-Flag construct plasmid was used in the titration. .. After 48 hr, Gaussia Luciferase activity and Cypridina Luciferase activity were measured with the BioLux Gaussia Luciferase Assay Kit (NEB) and the BioLux Cypridina Luciferase Assay Kit (NEB), respectively, on a TriStar LB 942 Multidetection Microplate Reader (Berthold Technologies GmbH & Co. KG, Bad Wildbad, Germany), according to the manufacturer’s protocols.

    Transfection:

    Article Title: Establishment and Characterization of the Reversibly Immortalized Mouse Fetal Heart Progenitors
    Article Snippet: Paragraph title: Transfection and Gaussia luciferase reporter assay ... At the indicated time points medium from the treated cells was collected for Gaussia luciferase assays using the Gaussia Luciferase Assay Kit (New England Biolabs) as described - .

    Article Title: An Enhancer Element Harboring Variants Associated with Systemic Lupus Erythematosus Engages the TNFAIP3 Promoter to Influence A20 Expression
    Article Snippet: Twenty fours hours post transfection, cells were treated with 1 ug/ml LPS for 24 hours or 50 ng/ml PMA/500 ng/ml ionomycin for 48 hours. .. To assay enhancer activity, Gaussia luciferase was analyzed from the cell culture media using BioLux GLuc assay kit (New England BioLab).

    Article Title: The P body protein LSm1 contributes to stimulation of hepatitis C virus translation, but not replication, by microRNA-122
    Article Snippet: Paragraph title: Cell culture and transfection ... Ten microliters of cell supernatant from triplicate wells was harvested at 1, 2, 3, 4, 6 and 24 h time points in luciferase lysis buffer (NEB), and assayed with Gaussia luciferase assay reagent (NEB).

    Article Title: Leukemia Inhibitory Factor Downregulates Human Papillomavirus-16 Oncogene Expression and Inhibits the Proliferation of Cervical Carcinoma Cells
    Article Snippet: For firefly luciferase, cells were seeded in 12-well plates at a density of 50,000/well and transfected with the indicated reporter plasmids using SuperFect (QIAGEN, Alameda, Calif, USA) according to manufacturer's instructions. .. Gaussia luciferase reagent (New England Biolabs) was added and the luminescence measured for 5 seconds.

    Article Title: Anti-Melanogenic Activity of Gagunin D, a Highly Oxygenated Diterpenoid from the Marine Sponge Phorbas sp., via Modulating Tyrosinase Expression and Degradation
    Article Snippet: Paragraph title: 4.10. Transient Transfection and Dual Luciferase Assay ... After a 24 h incubation with GD, the cell lysates were prepared and the Gaussia and firefly luciferase activity were determined by the Gaussia luciferase assay kit (New England BioLabs, Ipswich, MA, USA) and luciferase reporter assay system (Promega, Madison, WI, USA), respectively, according to the manufacturers’ protocols, using a luminometer (MicroLumat Plus, Berthold Technologies, Dortmund, Germany).

    Article Title: Reassortment compatibility between PB1, PB2, and HA genes of the two influenza B virus lineages in mammalian cells
    Article Snippet: To investigate the polymerase activity of viral RNP complexes (PB1, PB2, PA, and NP genes), we additionally constructed a Gaussia luciferase reporter plasmid (pPolI-GLuc) . .. The RNP complex gene and the pPolI-GLuc plasmids were transfected into 5 × 104 293T cells (ATCC, Manassas, VA, USA) using X-tremeGENE (Roche, Basel, Switzerland), and the cells were incubated at 37 °C for 24 h. After treating the cells using BioLux Gaussia Luciferase Assay Kit (New England Biolabs, Ipswich, MA, USA), luciferase activity was measured on a SpectraMax L plate reader (Molecular Devices, Sunnyvale, CA, USA). .. The cells transfected with empty plasmids were used as background luciferase expression.

    Article Title: Exogenous TNFR2 activation protects from acute GvHD via host T reg cell expansion
    Article Snippet: The next day, transfected cells were seeded into 24-well plates. .. After an additional day, the cells were incubated pairwise at 37°C for 2 h with increasing concentrations of GpL-TNC–mouse TNF and GpL-STAR2, and cell-bound luciferase activity was assayed using the Gaussia luciferase assay kit (New England Biolabs, Inc.) on a Lucy 2 luminometer (Anthos Labtec Instruments).

    Article Title: Genetically tunable frustration controls allostery in an intrinsically disordered transcription factor
    Article Snippet: For titration of the C3 isoform wild type and C3 C431Y & V435A & L436A mutant with the R domain-nuclear localization sequence-Flag construct, the method was the same as the competitive transfection assay described above, except up to 12 ng of the R domain-nuclear localization sequence-Flag construct plasmid was used in the titration. .. After 48 hr, Gaussia Luciferase activity and Cypridina Luciferase activity were measured with the BioLux Gaussia Luciferase Assay Kit (NEB) and the BioLux Cypridina Luciferase Assay Kit (NEB), respectively, on a TriStar LB 942 Multidetection Microplate Reader (Berthold Technologies GmbH & Co. KG, Bad Wildbad, Germany), according to the manufacturer’s protocols.

    Article Title: KLF4-dependent perivascular cell plasticity mediates pre-metastatic niche formation and metastasis
    Article Snippet: A KLF4 promoter-reporter plasmid that expresses the secretable Gaussia Luciferase was purchased from Genecopoeia, and transfected into plated MOVAS cells. .. 16 hours after protein-containing treatment, media was removed and assayed for luciferase activity using the BioLux Gaussia Luciferase Assay Kit (New England BioLabs).

    Article Title: Engineering circular RNA for potent and stable translation in eukaryotic cells
    Article Snippet: 25 µL of BioLux Gaussia Luciferase reagent including stabilizer (New England Biolabs) was added to each sample and luminescence was measured on an Infinite 200Pro Microplate Reader (Tecan) after 45 s. To detect luminescence from Firefly luciferase, 100 µL of Bright-Glo Luciferase reagent (Promega) was added to each well, mixed, and incubated for 5 min. 100 µL of the culture medium and luciferase reagent mix was then transferred to a flat-bottomed white-walled plate and luminescence was detected as described above. .. GFP fluorescence was detected 24 h post-transfection and images were taken using an EVOS FL cell imager (Invitrogen).

    Titration:

    Article Title: Genetically tunable frustration controls allostery in an intrinsically disordered transcription factor
    Article Snippet: For titration of the C3 isoform wild type and C3 C431Y & V435A & L436A mutant with the R domain-nuclear localization sequence-Flag construct, the method was the same as the competitive transfection assay described above, except up to 12 ng of the R domain-nuclear localization sequence-Flag construct plasmid was used in the titration. .. After 48 hr, Gaussia Luciferase activity and Cypridina Luciferase activity were measured with the BioLux Gaussia Luciferase Assay Kit (NEB) and the BioLux Cypridina Luciferase Assay Kit (NEB), respectively, on a TriStar LB 942 Multidetection Microplate Reader (Berthold Technologies GmbH & Co. KG, Bad Wildbad, Germany), according to the manufacturer’s protocols.

    FACS:

    Article Title: Entry Tropism of BK and Merkel Cell Polyomaviruses in Cell Culture
    Article Snippet: Cells were then analyzed by flow cytometery for GFP reporter gene expression in a FACS Canto II with HTS (BD Biosciences). .. A BMG Labtech Polarstar Optima luminometer was used to inject 50 µl of Gaussia Luciferase Assay Kit substrate (NEB), and light emission (in relative light units, RLUs) was measured according to manufacturer instructions.

    Plasmid Preparation:

    Article Title: An Enhancer Element Harboring Variants Associated with Systemic Lupus Erythematosus Engages the TNFAIP3 Promoter to Influence A20 Expression
    Article Snippet: Each plasmid was transiently co-transfected using FuGene HD (VWR, Radnor, PA) with a pGL3-promoter control plasmid for calculation of transfection efficiency and normalization (gift from Dr. Carol Webb, OMRF). .. To assay enhancer activity, Gaussia luciferase was analyzed from the cell culture media using BioLux GLuc assay kit (New England BioLab).

    Article Title: Anti-Melanogenic Activity of Gagunin D, a Highly Oxygenated Diterpenoid from the Marine Sponge Phorbas sp., via Modulating Tyrosinase Expression and Degradation
    Article Snippet: At 40%–50% confluency, the cells were washed with DPBS, and the Gaussia luciferase reporter construct pMITF-Gluc, pTYR-Gluc, and the control firefly luciferase vector (pGL3-FL) were transfected using FuGENE® HD Transfection Reagent (Promega, Madison, WI, USA). .. After a 24 h incubation with GD, the cell lysates were prepared and the Gaussia and firefly luciferase activity were determined by the Gaussia luciferase assay kit (New England BioLabs, Ipswich, MA, USA) and luciferase reporter assay system (Promega, Madison, WI, USA), respectively, according to the manufacturers’ protocols, using a luminometer (MicroLumat Plus, Berthold Technologies, Dortmund, Germany).

    Article Title: Reassortment compatibility between PB1, PB2, and HA genes of the two influenza B virus lineages in mammalian cells
    Article Snippet: To investigate the polymerase activity of viral RNP complexes (PB1, PB2, PA, and NP genes), we additionally constructed a Gaussia luciferase reporter plasmid (pPolI-GLuc) . .. The RNP complex gene and the pPolI-GLuc plasmids were transfected into 5 × 104 293T cells (ATCC, Manassas, VA, USA) using X-tremeGENE (Roche, Basel, Switzerland), and the cells were incubated at 37 °C for 24 h. After treating the cells using BioLux Gaussia Luciferase Assay Kit (New England Biolabs, Ipswich, MA, USA), luciferase activity was measured on a SpectraMax L plate reader (Molecular Devices, Sunnyvale, CA, USA).

    Article Title: Exogenous TNFR2 activation protects from acute GvHD via host T reg cell expansion
    Article Snippet: To analyze the binding of wild-type mouse TNF and mouse STAR2 to mouse TNFR2, HEK293 cells were electroporated with an empty vector or a mouse TNFR2 expression plasmid. .. After an additional day, the cells were incubated pairwise at 37°C for 2 h with increasing concentrations of GpL-TNC–mouse TNF and GpL-STAR2, and cell-bound luciferase activity was assayed using the Gaussia luciferase assay kit (New England Biolabs, Inc.) on a Lucy 2 luminometer (Anthos Labtec Instruments).

    Article Title: Genetically tunable frustration controls allostery in an intrinsically disordered transcription factor
    Article Snippet: For titration of the C3 isoform wild type and C3 C431Y & V435A & L436A mutant with the R domain-nuclear localization sequence-Flag construct, the method was the same as the competitive transfection assay described above, except up to 12 ng of the R domain-nuclear localization sequence-Flag construct plasmid was used in the titration. .. After 48 hr, Gaussia Luciferase activity and Cypridina Luciferase activity were measured with the BioLux Gaussia Luciferase Assay Kit (NEB) and the BioLux Cypridina Luciferase Assay Kit (NEB), respectively, on a TriStar LB 942 Multidetection Microplate Reader (Berthold Technologies GmbH & Co. KG, Bad Wildbad, Germany), according to the manufacturer’s protocols.

    Article Title: KLF4-dependent perivascular cell plasticity mediates pre-metastatic niche formation and metastasis
    Article Snippet: Paragraph title: KLF4 knock-down and reporter plasmid ... 16 hours after protein-containing treatment, media was removed and assayed for luciferase activity using the BioLux Gaussia Luciferase Assay Kit (New England BioLabs).

    Article Title: Altered expression and function of regulator of G-protein signaling-17 (RGS17) in hepatocellular carcinoma
    Article Snippet: IMPROM II™ transcription system, psiLentGene vector, GoTaq green master mix, DualGlo Luciferase Assay, and the cAMP-Glo kit were purchased from Promega (Madison, WI). .. The pGLuc plasmid (possessing Gaussian luciferase) and a BioLux Gaussia Luciferase Assay Kit were purchased from New England Biolabs (Ipswich, MA). .. Antibodies against SUMO-1, SUMO-1/ 3, β-actin and Gqα were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

    Software:

    Article Title: Exogenous TNFR2 activation protects from acute GvHD via host T reg cell expansion
    Article Snippet: After an additional day, the cells were incubated pairwise at 37°C for 2 h with increasing concentrations of GpL-TNC–mouse TNF and GpL-STAR2, and cell-bound luciferase activity was assayed using the Gaussia luciferase assay kit (New England Biolabs, Inc.) on a Lucy 2 luminometer (Anthos Labtec Instruments). .. Wells were then incubated with increasing concentrations of GpL–mouse TNF and GpL-STAR2 for 1 h at 37°C, and the well-associated luciferase activity was quantified using the Gaussia luciferase assay kit.

    Recombinant:

    Article Title: KLF4-dependent perivascular cell plasticity mediates pre-metastatic niche formation and metastasis
    Article Snippet: 72 hours post-siRNA treatment, media was replaced with serum-free media containing recombinant proteins or tumor conditioned media. .. 16 hours after protein-containing treatment, media was removed and assayed for luciferase activity using the BioLux Gaussia Luciferase Assay Kit (New England BioLabs).

    Concentration Assay:

    Article Title: Establishment and Characterization of the Reversibly Immortalized Mouse Fetal Heart Progenitors
    Article Snippet: At 16h, the transfected cells were replated to 24-well plates and treated with all-trans retinoic acid (RA, final concentration=1μM; from 0.5mM stock prepared in DMSO) or DMSO. .. At the indicated time points medium from the treated cells was collected for Gaussia luciferase assays using the Gaussia Luciferase Assay Kit (New England Biolabs) as described - .

    Lysis:

    Article Title: The P body protein LSm1 contributes to stimulation of hepatitis C virus translation, but not replication, by microRNA-122
    Article Snippet: A fraction of cells electroporated with Bi-Gluc-H77C(1a)/JFH1 RNA were plated in 24-well plates for luciferase assays. .. Ten microliters of cell supernatant from triplicate wells was harvested at 1, 2, 3, 4, 6 and 24 h time points in luciferase lysis buffer (NEB), and assayed with Gaussia luciferase assay reagent (NEB). .. Immunoprecipitation of Argonaute complexes was performed on electroporated cells cultured in 10-cm plates as described in , except that the monoclonal antibody 11A9 (Sigma SAB4200085) was used to immunoprecipitate Ago2.

    Article Title: Leukemia Inhibitory Factor Downregulates Human Papillomavirus-16 Oncogene Expression and Inhibits the Proliferation of Cervical Carcinoma Cells
    Article Snippet: Luciferase was released from cells using the supplied lysis buffer and luminescence measured using luciferase substrate (New England Biolabs, Ipswich, Mass, USA). .. Gaussia luciferase reagent (New England Biolabs) was added and the luminescence measured for 5 seconds.

    Standard Deviation:

    Article Title: Synthetic mammalian trigger-controlled bipartite transcription factors
    Article Snippet: GLuc was quantified using the BioLux® GLuc assay (E3300S, New England Biolabs) and the EnVision 2104 multilabel reader (maximum emission 482 nm). .. GLuc was quantified using the BioLux® GLuc assay (E3300S, New England Biolabs) and the EnVision 2104 multilabel reader (maximum emission 482 nm).

    Activity Assay:

    Article Title: Cell-to-Cell Transmission Can Overcome Multiple Donor and Target Cell Barriers Imposed on Cell-Free HIV
    Article Snippet: Antibody against HIV Gag (clone KC57) was purchased from Beckman Coulter. .. Luciferase activity was measured using the BioLux Gaussia Luciferase Assay Kit (New England Biolabs). .. Co-culture experiments were performed using HEK293 cells transiently transfected with the plasmids encoding full length HIV (molecular clone NL4-3) and HIV-inGLuc (at a ratio of 6∶1) using Fugene 6 Transfection Reagent (Roche) or XtremeGene9 (Roche).

    Article Title: Class A Scavenger Receptor 1 (MSR1) Restricts Hepatitis C Virus Replication by Mediating Toll-like Receptor 3 Recognition of Viral RNAs Produced in Neighboring Cells
    Article Snippet: Cells were infected at an m.o.i. of 1. .. GLuc activity in supernatants was measured by BioLux Gaussia Luciferase Assay Kit (New England Biolabs, Ipswich, MA) using a Synergy2 multi-mode microplate reader (BioTek, Winooski, VT). .. HJ3-5/5A-YFP is another derivative of HJ3-5 containing yellow fluorescent protein (YFP) coding sequence fused to NS5A sequence .

    Article Title: Establishment and Characterization of the Reversibly Immortalized Mouse Fetal Heart Progenitors
    Article Snippet: At the indicated time points medium from the treated cells was collected for Gaussia luciferase assays using the Gaussia Luciferase Assay Kit (New England Biolabs) as described - . .. At the indicated time points medium from the treated cells was collected for Gaussia luciferase assays using the Gaussia Luciferase Assay Kit (New England Biolabs) as described - .

    Article Title: Synthetic mammalian trigger-controlled bipartite transcription factors
    Article Snippet: GLuc was quantified using the BioLux® GLuc assay (E3300S, New England Biolabs) and the EnVision 2104 multilabel reader (maximum emission 482 nm). .. GLuc was quantified using the BioLux® GLuc assay (E3300S, New England Biolabs) and the EnVision 2104 multilabel reader (maximum emission 482 nm).

    Article Title: In Vitro Screening for Compounds That Enhance Human L1 Mobilization
    Article Snippet: Luminescence was measured with the Multilabel Counter 1420 ARVO using the BioLux Gaussia luciferase assay kit (New England BioLabs) and BioLux Cypridina luciferase assay kit (New England BioLabs). .. Luminescence was measured with the Multilabel Counter 1420 ARVO using the BioLux Gaussia luciferase assay kit (New England BioLabs) and BioLux Cypridina luciferase assay kit (New England BioLabs).

    Article Title: An Enhancer Element Harboring Variants Associated with Systemic Lupus Erythematosus Engages the TNFAIP3 Promoter to Influence A20 Expression
    Article Snippet: Twenty fours hours post transfection, cells were treated with 1 ug/ml LPS for 24 hours or 50 ng/ml PMA/500 ng/ml ionomycin for 48 hours. .. To assay enhancer activity, Gaussia luciferase was analyzed from the cell culture media using BioLux GLuc assay kit (New England BioLab). .. To measure transfection efficiency, cells were lysed and firefly luciferase activity was measured using the Luciferase Assay System (Promega, Madison, WI).

    Article Title: SRA Regulates Adipogenesis by Modulating p38/JNK Phosphorylation and Stimulating Insulin Receptor Gene Expression and Downstream Signaling
    Article Snippet: Sequences of the qPCR primers are available upon request or have been previously described . .. 3T3-L1 preadipocytes were cotransfected with pIRP-GLuc or pGluc-Basic (100 ng) and either pSCT or pSCT-SRA, pSCT-SRAP or pSCT-SRAP silent mutant plasmids for 48 hr using Lipofectamine Plus Reagent (Invitrogen) in 24 well plates, and luciferase activity was measured using a BioLux Gaussia Luciferase Assay Kit (New England Biolabs, Inc.). .. Results are presented as the mean±SD.

    Article Title: Anti-Melanogenic Activity of Gagunin D, a Highly Oxygenated Diterpenoid from the Marine Sponge Phorbas sp., via Modulating Tyrosinase Expression and Degradation
    Article Snippet: At 40%–50% confluency, the cells were washed with DPBS, and the Gaussia luciferase reporter construct pMITF-Gluc, pTYR-Gluc, and the control firefly luciferase vector (pGL3-FL) were transfected using FuGENE® HD Transfection Reagent (Promega, Madison, WI, USA). .. After a 24 h incubation with GD, the cell lysates were prepared and the Gaussia and firefly luciferase activity were determined by the Gaussia luciferase assay kit (New England BioLabs, Ipswich, MA, USA) and luciferase reporter assay system (Promega, Madison, WI, USA), respectively, according to the manufacturers’ protocols, using a luminometer (MicroLumat Plus, Berthold Technologies, Dortmund, Germany). .. The reconstructed human skin model, Neoderm® -ME, was purchased from Tego Science (Seoul, Korea) and was analyzed according to the manufacturer’s instructions.

    Article Title: Reassortment compatibility between PB1, PB2, and HA genes of the two influenza B virus lineages in mammalian cells
    Article Snippet: To investigate the polymerase activity of viral RNP complexes (PB1, PB2, PA, and NP genes), we additionally constructed a Gaussia luciferase reporter plasmid (pPolI-GLuc) . .. The RNP complex gene and the pPolI-GLuc plasmids were transfected into 5 × 104 293T cells (ATCC, Manassas, VA, USA) using X-tremeGENE (Roche, Basel, Switzerland), and the cells were incubated at 37 °C for 24 h. After treating the cells using BioLux Gaussia Luciferase Assay Kit (New England Biolabs, Ipswich, MA, USA), luciferase activity was measured on a SpectraMax L plate reader (Molecular Devices, Sunnyvale, CA, USA). .. The cells transfected with empty plasmids were used as background luciferase expression.

    Article Title: Exogenous TNFR2 activation protects from acute GvHD via host T reg cell expansion
    Article Snippet: The next day, transfected cells were seeded into 24-well plates. .. After an additional day, the cells were incubated pairwise at 37°C for 2 h with increasing concentrations of GpL-TNC–mouse TNF and GpL-STAR2, and cell-bound luciferase activity was assayed using the Gaussia luciferase assay kit (New England Biolabs, Inc.) on a Lucy 2 luminometer (Anthos Labtec Instruments). .. Specific binding values were calculated for the two GpL fusion proteins by subtracting the values obtained for empty vector–transfected cells (nonspecific binding) from the corresponding values verified for the TNFR2 transfectants.

    Article Title: Genetically tunable frustration controls allostery in an intrinsically disordered transcription factor
    Article Snippet: For titration of the C3 isoform wild type and C3 C431Y & V435A & L436A mutant with the R domain-nuclear localization sequence-Flag construct, the method was the same as the competitive transfection assay described above, except up to 12 ng of the R domain-nuclear localization sequence-Flag construct plasmid was used in the titration. .. After 48 hr, Gaussia Luciferase activity and Cypridina Luciferase activity were measured with the BioLux Gaussia Luciferase Assay Kit (NEB) and the BioLux Cypridina Luciferase Assay Kit (NEB), respectively, on a TriStar LB 942 Multidetection Microplate Reader (Berthold Technologies GmbH & Co. KG, Bad Wildbad, Germany), according to the manufacturer’s protocols. .. In each experiment, the Gaussia luciferase activity (normalized by the Cypridina luciferase activity) was measured in triplicate and averaged.

    Article Title: KLF4-dependent perivascular cell plasticity mediates pre-metastatic niche formation and metastasis
    Article Snippet: 72 hours post-siRNA treatment, media was replaced with serum-free media containing recombinant proteins or tumor conditioned media. .. 16 hours after protein-containing treatment, media was removed and assayed for luciferase activity using the BioLux Gaussia Luciferase Assay Kit (New England BioLabs). .. Reporter plasmid activity is reported in luminescence as compared to empty plasmid control.

    Article Title: The effect of glycosaminoglycan content on polyethylenimine-based gene delivery within three-dimensional collagen-GAG scaffolds
    Article Snippet: Luciferase expression was subsequently quantified over time (through 14 days) after cell seeding using the BioLux Gaussia Luciferase Assay Kit (New England BioLabs). .. Luciferase expression was subsequently quantified over time (through 14 days) after cell seeding using the BioLux Gaussia Luciferase Assay Kit (New England BioLabs).

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    New England Biolabs gaussia luciferase
    Gaussia Luciferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gaussia luciferase/product/New England Biolabs
    Average 99 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    gaussia luciferase - by Bioz Stars, 2019-07
    99/100 stars
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