ptwin1 (New England Biolabs)


Structured Review

Ptwin1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ptwin1/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Intein-mediated recombinant expression of monomeric B22Asp desB30 insulin"
Article Title: Intein-mediated recombinant expression of monomeric B22Asp desB30 insulin
Journal: BMC Biotechnology
doi: 10.1186/s12896-020-0598-3

Figure Legend Snippet: Purification of DPIP by the IMPACT-TWIN system. a : Scheme of the protein expression and purification. b : E. coli strain BL21 (DE3) transformed with pTWIN1-DPIP was cultured, induced with IPTG and fractionated to purify the recombinant fusion protein. Fractions were analyzed by 12% SDS–PAGE. M: MW marker (97, 66, 43, 31, 24, 14 kDa), 1: E coli lysate before induction, 2: lysate after induction, 3: soluble fraction after induction. 4: redissolved inclusion body before dialysis, 5: redissolved fraction after dialysis, 6: redissolved, dialyzed fraction flow through. An arrow marks the full-length fusion protein
Techniques Used: Purification, Expressing, Transformation Assay, Cell Culture, Recombinant, SDS Page, Marker, Flow Cytometry