ptwin1 (New England Biolabs)
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Name:
pTWIN1 Vector DNA
Description:
pTWIN1 Vector DNA 10 ug
Catalog Number:
N6951S
Price:
127
Category:
E coli Expression Vectors
Size:
10 ug
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Structured Review
New England Biolabs
ptwin1
pTWIN1 Vector DNA 10 ug
https://www.bioz.com/result/ptwin1/product/New England Biolabs
Average 98 stars, based on 1 article reviews
Price from $9.99 to $1999.99
pTWIN1 Vector DNA 10 ug
https://www.bioz.com/result/ptwin1/product/New England Biolabs
Average 98 stars, based on 1 article reviews
Price from $9.99 to $1999.99
ptwin1 - by Bioz Stars,
2021-04
98/100 stars
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1) Product Images from "Intein-mediated recombinant expression of monomeric B22Asp desB30 insulin"
Article Title: Intein-mediated recombinant expression of monomeric B22Asp desB30 insulin
Journal: BMC Biotechnology
doi: 10.1186/s12896-020-0598-3
Figure Legend Snippet: Purification of DPIP by the IMPACT-TWIN system. a : Scheme of the protein expression and purification. b : E. coli strain BL21 (DE3) transformed with pTWIN1-DPIP was cultured, induced with IPTG and fractionated to purify the recombinant fusion protein. Fractions were analyzed by 12% SDS–PAGE. M: MW marker (97, 66, 43, 31, 24, 14 kDa), 1: E coli lysate before induction, 2: lysate after induction, 3: soluble fraction after induction. 4: redissolved inclusion body before dialysis, 5: redissolved fraction after dialysis, 6: redissolved, dialyzed fraction flow through. An arrow marks the full-length fusion protein
Techniques Used: Purification, Expressing, Transformation Assay, Cell Culture, Recombinant, SDS Page, Marker, Flow Cytometry
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We chose Article Title: Colonisation Factor CD0873, an Attractive Oral Vaccine Candidate against Clostridioides difficile Article Snippet: The amplified DNA was visualised by electrophoresis with 1% w/v agarose gels. .. Engineering of Antigen Expression Constructs The Intein Mediated Purification with an Affinity Chitin-binding Tag-Two Intein (IMPACT-TWIN) system was used (NEB), specifically the Article Title: Intein-mediated recombinant expression of monomeric B22Asp desB30 insulin Article Snippet: Plasmid construction The coding sequence of the insulin precursor (PIP) was used as a template for subsequent site-directed-mutagenesis amplification (B22D) with Pfu under standard conditions using the primer pair M1/M2 (sequence: 5′- TTGGTCTGTGGTGAAGACGGTTTCTTCTACACC-3′ and 5′-GGTGTAGAAGAAACCGTCTTCACCACAGACCAA-3′). .. The coding sequence of B22D desB30 was flanked by 5′ NcoI and 3′ PstI restriction sites for inserting into the Purification:Article Title: A DNA Vaccine Encoding for TcSSP4 Induces Protection against Acute and Chronic Infection in Experimental Chagas Disease Article Snippet: Hideyo Noguchi”, Universidad Autónoma de Yucatán, Mérida, Yucatán, México] was maintained in vivo by serial passages of blood form trypomastigotes in BALB/c mice and by weekly in vitro passages of epimastigotes in Liver Infusion Tryptose (LIT) with 10 % of fetal calf serum (Gibco, Invitrogen), as described . .. Plasmid construction and recombinant protein expression and purification DNA encoding for the Tc SSP4 protein (GeneBankTM database accession number AF480943) was obtained from the plasmid pMAL-TcSSP4 as a 2.2 Eco RI fragment and ligated in the Eco RI site of plasmids pBk-CMV (Stratagene, La Jolla , CA) and Article Title: Colonisation Factor CD0873, an Attractive Oral Vaccine Candidate against Clostridioides difficile Article Snippet: The amplified DNA was visualised by electrophoresis with 1% w/v agarose gels. .. Engineering of Antigen Expression Constructs The Intein Mediated Purification with an Affinity Chitin-binding Tag-Two Intein (IMPACT-TWIN) system was used (NEB), specifically the Labeling:Article Title: Segmental Isotopic Labeling of Proteins for Nuclear Magnetic Resonance Article Snippet: From preliminary studies, we knew the refolding of Csk is very difficult, so we decided use expressed protein ligation do the segmental labeling. .. We chose Construct:Article Title: Colonisation Factor CD0873, an Attractive Oral Vaccine Candidate against Clostridioides difficile Article Snippet: The amplified DNA was visualised by electrophoresis with 1% w/v agarose gels. .. Engineering of Antigen Expression Constructs The Intein Mediated Purification with an Affinity Chitin-binding Tag-Two Intein (IMPACT-TWIN) system was used (NEB), specifically the Modification:Article Title: Colonisation Factor CD0873, an Attractive Oral Vaccine Candidate against Clostridioides difficile Article Snippet: The amplified DNA was visualised by electrophoresis with 1% w/v agarose gels. .. Engineering of Antigen Expression Constructs The Intein Mediated Purification with an Affinity Chitin-binding Tag-Two Intein (IMPACT-TWIN) system was used (NEB), specifically the Sequencing:Article Title: Modulation of Antigen Display on PapMV Nanoparticles Influences Its Immunogenicity Article Snippet: Sortase A and Nanoparticle Protein Production, Purification, and Self-Assembly Methods for the production and purification of the Sortase A transpeptidase, PapMV, and PapMV-C have been reported previously [ ]. .. The PapMV-N CP sequence, containing four glycines at its N-terminus and a 6His-tag at its C-terminus, was genetically inserted at the C-terminus of the intein sequence in the Article Title: Intein-mediated recombinant expression of monomeric B22Asp desB30 insulin Article Snippet: Plasmid construction The coding sequence of the insulin precursor (PIP) was used as a template for subsequent site-directed-mutagenesis amplification (B22D) with Pfu under standard conditions using the primer pair M1/M2 (sequence: 5′- TTGGTCTGTGGTGAAGACGGTTTCTTCTACACC-3′ and 5′-GGTGTAGAAGAAACCGTCTTCACCACAGACCAA-3′). .. The coding sequence of B22D desB30 was flanked by 5′ NcoI and 3′ PstI restriction sites for inserting into the |
