ptyb21 (New England Biolabs)
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New England Biolabs manufactures this product 
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Name:
pTYB21 Vector
Description:
Catalog Number:
N6709
Price:
136
Category:
Nucleic Acids
Applications:
Proteomics & Glycomics
Size:
10 µg
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Structured Review
New England Biolabs
ptyb21
https://www.bioz.com/result/ptyb21/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
https://www.bioz.com/result/ptyb21/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
ptyb21 - by Bioz Stars,
2021-09
94/100 stars
Images
1) Product Images from "Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity"
Article Title: Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity
Journal: Iranian Journal of Pharmaceutical Research : IJPR
doi: 10.22037/ijpr.2019.112397.13734
Figure Legend Snippet: The structure of recombinant vector pTYB21 for novel designed AMP (P24). (A) The structure of pTYB21; the important parts are outlined with red box. (B) The sequence of first 5 amino acids at MCS of pTYB21, was added to the N-terminal of P19, indicated in red box
Techniques Used: Recombinant, Plasmid Preparation, Sequencing
2) Product Images from "Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity"
Article Title: Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity
Journal: Iranian Journal of Pharmaceutical Research : IJPR
doi: 10.22037/ijpr.2019.112397.13734
Figure Legend Snippet: The structure of recombinant vector pTYB21 for novel designed AMP (P24). (A) The structure of pTYB21; the important parts are outlined with red box. (B) The sequence of first 5 amino acids at MCS of pTYB21, was added to the N-terminal of P19, indicated in red box
Techniques Used: Recombinant, Plasmid Preparation, Sequencing
3) Product Images from "Bile salts and alkaline pH reciprocally modulate the interaction between the periplasmic domains of Vibrio cholerae ToxR and ToxS"
Article Title: Bile salts and alkaline pH reciprocally modulate the interaction between the periplasmic domains of Vibrio cholerae ToxR and ToxS
Journal: Molecular microbiology
doi: 10.1111/mmi.13699
Figure Legend Snippet: Cloning and purification of the ToxR and ToxS periplasmic domains. A. Schematic of ToxR (top) and ToxS (bottom) showing domain boundaries. ToxR contains a cytoplasmic DNA binding domain, while ToxS has a 5 amino acid cytoplasmic tail. Both proteins contain a single pass transmembrane domain along with a C-terminal periplasmic domain. DNA encoding the periplasmic domains of ToxR (T199-E294) and ToxS (S25-S173) were cloned in frame into pTYB21, which contains a N-terminal chitin binding domain intein tag. The amino acid sequences of the purified constructs are shown with the periplasmic domain sequences in bold, and the N-terminal five amino acids leftover from the intein tag after cleavage in gray. B. A Colloidal Blue stained 16% SDS-PAGE gel of 1 μg of purified ToxRp and ToxSp.
Techniques Used: Clone Assay, Purification, Binding Assay, Construct, Staining, SDS Page
Related Articles
Polymerase Chain Reaction:Article Title: Substrates of the chloroplast small heat shock proteins 22E/F point to thermolability as a regulative switch for heat acclimation in Chlamydomonas reinhardtii Article Snippet: .. The 1490-bp PCR product was digested with SapI and NdeI and cloned into Article Title: Structural and molecular comparison of bacterial and eukaryotic trigger factors Article Snippet: .. The TF sequence from E . coli was amplified by PCR from DH5alpha genomic DNA with primers (5′-GGGGCATATGCAAGTTTCAGTTGAAACCAC-3′) and (5′-CCCCGGATCCTTACGCCTGCTGGTTCATC-3′) and cloned with NdeI and BamHI into Clone Assay:Article Title: Substrates of the chloroplast small heat shock proteins 22E/F point to thermolability as a regulative switch for heat acclimation in Chlamydomonas reinhardtii Article Snippet: .. The 1490-bp PCR product was digested with SapI and NdeI and cloned into Article Title: Molecular Characterization of a Date Palm Vascular Highway 1-Interacting Kinase (PdVIK) under Abiotic Stresses Article Snippet: .. Production of Recombinant PdVIK Protein in E. coli The full-length cDNA of PdVIK was amplified using primers containing Nco I and Bam HI sites , and the amplicon was cloned into a Article Title: Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity Article Snippet: .. The P24 gene was designed with NdeI restriction site at 5’ end and EcoRI restriction site at the 3´end of the gene for directed cloning into the Article Title: Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity Article Snippet: .. Cloning and transformation of AMP gene-containing construct The P24 gene was digested with NdeI at 5’ end and EcoRI at the 3´end of the gene for site-directed cloning into the Article Title: The Role of Plastidic Trigger Factor Serving Protein Biogenesis in Green Algae and Land Plants 1The Role of Plastidic Trigger Factor Serving Protein Biogenesis in Green Algae and Land Plants 1 [OPEN] Article Snippet: .. Mature cpSRP54 (Cre11.g479750, lacking the predicted chloroplast transit peptide) was cloned from Chlamydomonas cDNA, cut with Nde I and Eco RI, and cloned into Article Title: Structural and molecular comparison of bacterial and eukaryotic trigger factors Article Snippet: .. The TF sequence from E . coli was amplified by PCR from DH5alpha genomic DNA with primers (5′-GGGGCATATGCAAGTTTCAGTTGAAACCAC-3′) and (5′-CCCCGGATCCTTACGCCTGCTGGTTCATC-3′) and cloned with NdeI and BamHI into Recombinant:Article Title: Molecular Characterization of a Date Palm Vascular Highway 1-Interacting Kinase (PdVIK) under Abiotic Stresses Article Snippet: .. Production of Recombinant PdVIK Protein in E. coli The full-length cDNA of PdVIK was amplified using primers containing Nco I and Bam HI sites , and the amplicon was cloned into a Amplification:Article Title: Molecular Characterization of a Date Palm Vascular Highway 1-Interacting Kinase (PdVIK) under Abiotic Stresses Article Snippet: .. Production of Recombinant PdVIK Protein in E. coli The full-length cDNA of PdVIK was amplified using primers containing Nco I and Bam HI sites , and the amplicon was cloned into a Article Title: Structural and molecular comparison of bacterial and eukaryotic trigger factors Article Snippet: .. The TF sequence from E . coli was amplified by PCR from DH5alpha genomic DNA with primers (5′-GGGGCATATGCAAGTTTCAGTTGAAACCAC-3′) and (5′-CCCCGGATCCTTACGCCTGCTGGTTCATC-3′) and cloned with NdeI and BamHI into Plasmid Preparation:Article Title: Molecular Characterization of a Date Palm Vascular Highway 1-Interacting Kinase (PdVIK) under Abiotic Stresses Article Snippet: .. Production of Recombinant PdVIK Protein in E. coli The full-length cDNA of PdVIK was amplified using primers containing Nco I and Bam HI sites , and the amplicon was cloned into a Article Title: Heterotypic Assembly Mechanism Regulates CHIP E3 Ligase Activity Article Snippet: .. For the in vitro ubiquitylation reactions, we first generated a Article Title: Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity Article Snippet: .. In Vitro:Article Title: Heterotypic Assembly Mechanism Regulates CHIP E3 Ligase Activity Article Snippet: .. For the in vitro ubiquitylation reactions, we first generated a Generated:Article Title: Heterotypic Assembly Mechanism Regulates CHIP E3 Ligase Activity Article Snippet: .. For the in vitro ubiquitylation reactions, we first generated a Expressing:Article Title: Heterotypic Assembly Mechanism Regulates CHIP E3 Ligase Activity Article Snippet: .. For the in vitro ubiquitylation reactions, we first generated a Article Title: The Role of Plastidic Trigger Factor Serving Protein Biogenesis in Green Algae and Land Plants 1The Role of Plastidic Trigger Factor Serving Protein Biogenesis in Green Algae and Land Plants 1 [OPEN] Article Snippet: .. Mature cpSRP54 (Cre11.g479750, lacking the predicted chloroplast transit peptide) was cloned from Chlamydomonas cDNA, cut with Nde I and Eco RI, and cloned into Purification:Article Title: Heterotypic Assembly Mechanism Regulates CHIP E3 Ligase Activity Article Snippet: .. For the in vitro ubiquitylation reactions, we first generated a Article Title: The Role of Plastidic Trigger Factor Serving Protein Biogenesis in Green Algae and Land Plants 1The Role of Plastidic Trigger Factor Serving Protein Biogenesis in Green Algae and Land Plants 1 [OPEN] Article Snippet: .. Mature cpSRP54 (Cre11.g479750, lacking the predicted chloroplast transit peptide) was cloned from Chlamydomonas cDNA, cut with Nde I and Eco RI, and cloned into Transformation Assay:Article Title: Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity Article Snippet: .. Cloning and transformation of AMP gene-containing construct The P24 gene was digested with NdeI at 5’ end and EcoRI at the 3´end of the gene for site-directed cloning into the Article Title: The Role of Plastidic Trigger Factor Serving Protein Biogenesis in Green Algae and Land Plants 1The Role of Plastidic Trigger Factor Serving Protein Biogenesis in Green Algae and Land Plants 1 [OPEN] Article Snippet: .. Mature cpSRP54 (Cre11.g479750, lacking the predicted chloroplast transit peptide) was cloned from Chlamydomonas cDNA, cut with Nde I and Eco RI, and cloned into Construct:Article Title: Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity Article Snippet: .. Cloning and transformation of AMP gene-containing construct The P24 gene was digested with NdeI at 5’ end and EcoRI at the 3´end of the gene for site-directed cloning into the Sequencing:Article Title: Structural and molecular comparison of bacterial and eukaryotic trigger factors Article Snippet: .. The TF sequence from E . coli was amplified by PCR from DH5alpha genomic DNA with primers (5′-GGGGCATATGCAAGTTTCAGTTGAAACCAC-3′) and (5′-CCCCGGATCCTTACGCCTGCTGGTTCATC-3′) and cloned with NdeI and BamHI into |