ptyb21 (New England Biolabs)


Name:
pTYB21 Vector
Description:
pTYB21 Vector 10 ug
Catalog Number:
n6709s
Price:
133
Size:
10 ug
Category:
E coli Expression Vectors
|
Buy from Supplier |
Structured Review

pTYB21 Vector 10 ug
https://www.bioz.com/result/ptyb21/product/New England Biolabs
Average 94 stars, based on 18 article reviews
Price from $9.99 to $1999.99
Related Products / Commonly Used Together
Images
1) Product Images from "Bile salts and alkaline pH reciprocally modulate the interaction between the periplasmic domains of Vibrio cholerae ToxR and ToxS"
Article Title: Bile salts and alkaline pH reciprocally modulate the interaction between the periplasmic domains of Vibrio cholerae ToxR and ToxS
Journal: Molecular microbiology
doi: 10.1111/mmi.13699

Figure Legend Snippet: Cloning and purification of the ToxR and ToxS periplasmic domains. A. Schematic of ToxR (top) and ToxS (bottom) showing domain boundaries. ToxR contains a cytoplasmic DNA binding domain, while ToxS has a 5 amino acid cytoplasmic tail. Both proteins contain a single pass transmembrane domain along with a C-terminal periplasmic domain. DNA encoding the periplasmic domains of ToxR (T199-E294) and ToxS (S25-S173) were cloned in frame into pTYB21, which contains a N-terminal chitin binding domain intein tag. The amino acid sequences of the purified constructs are shown with the periplasmic domain sequences in bold, and the N-terminal five amino acids leftover from the intein tag after cleavage in gray. B. A Colloidal Blue stained 16% SDS-PAGE gel of 1 μg of purified ToxRp and ToxSp.
Techniques Used: Clone Assay, Purification, Binding Assay, Construct, Staining, SDS Page
2) Product Images from "Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity"
Article Title: Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity
Journal: Iranian Journal of Pharmaceutical Research : IJPR
doi: 10.22037/ijpr.2019.112397.13734

Figure Legend Snippet: The structure of recombinant vector pTYB21 for novel designed AMP (P24). (A) The structure of pTYB21; the important parts are outlined with red box. (B) The sequence of first 5 amino acids at MCS of pTYB21, was added to the N-terminal of P19, indicated in red box
Techniques Used: Recombinant, Plasmid Preparation, Sequencing
3) Product Images from "Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity"
Article Title: Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity
Journal: Iranian Journal of Pharmaceutical Research : IJPR
doi: 10.22037/ijpr.2019.112397.13734

Figure Legend Snippet: The structure of recombinant vector pTYB21 for novel designed AMP (P24). (A) The structure of pTYB21; the important parts are outlined with red box. (B) The sequence of first 5 amino acids at MCS of pTYB21, was added to the N-terminal of P19, indicated in red box
Techniques Used: Recombinant, Plasmid Preparation, Sequencing
4) Product Images from "Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity"
Article Title: Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity
Journal: Iranian Journal of Pharmaceutical Research : IJPR
doi: 10.22037/ijpr.2019.112397.13734

Figure Legend Snippet: The structure of recombinant vector pTYB21 for novel designed AMP (P24). (A) The structure of pTYB21; the important parts are outlined with red box. (B) The sequence of first 5 amino acids at MCS of pTYB21, was added to the N-terminal of P19, indicated in red box
Techniques Used: Recombinant, Plasmid Preparation, Sequencing
5) Product Images from "Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity"
Article Title: Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity
Journal: Iranian Journal of Pharmaceutical Research : IJPR
doi: 10.22037/ijpr.2019.112397.13734

Figure Legend Snippet: The structure of recombinant vector pTYB21 for novel designed AMP (P24). (A) The structure of pTYB21; the important parts are outlined with red box. (B) The sequence of first 5 amino acids at MCS of pTYB21, was added to the N-terminal of P19, indicated in red box
Techniques Used: Recombinant, Plasmid Preparation, Sequencing
Related Articles
Clone Assay:Article Title: Crystal Structure of the Human Fatty Acid Synthase Enoyl-Acyl Carrier Protein-Reductase Domain Complexed with Triclosan Reveals Allosteric Protein-Protein Interface Inhibition * Article Snippet: .. The cDNA was cloned into a Article Title: Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity Article Snippet: .. The P24 gene was designed with NdeI restriction site at 5’ end and EcoRI restriction site at the 3´end of the gene for directed cloning into the Article Title: A Critical Role of Zinc Importer AdcABC in Group A Streptococcus-Host Interactions During Infection and Its Implications for Vaccine Development Article Snippet: .. 2.12 Overexpression and Purification of AdcA-NTD The coding sequence of the extracellular domain of adcA without the secretion signal sequence (amino acids 32-320) from strain MGAS10870 was amplified and cloned into Nde I and Bam HI restriction sites of Article Title: Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity Article Snippet: .. Cloning and transformation of AMP gene-containing construct The P24 gene was digested with NdeI at 5’ end and EcoRI at the 3´end of the gene for site-directed cloning into the Amplification:Article Title: A Critical Role of Zinc Importer AdcABC in Group A Streptococcus-Host Interactions During Infection and Its Implications for Vaccine Development Article Snippet: .. 2.12 Overexpression and Purification of AdcA-NTD The coding sequence of the extracellular domain of adcA without the secretion signal sequence (amino acids 32-320) from strain MGAS10870 was amplified and cloned into Nde I and Bam HI restriction sites of Article Title: Recombinant broad-range phospholipase C from Listeriamonocytogenes exhibits optimal activity at acidic pH Article Snippet: .. The amplified fragment was ligated into Construct:Article Title: Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity Article Snippet: .. Cloning and transformation of AMP gene-containing construct The P24 gene was digested with NdeI at 5’ end and EcoRI at the 3´end of the gene for site-directed cloning into the Purification:Article Title: A Critical Role of Zinc Importer AdcABC in Group A Streptococcus-Host Interactions During Infection and Its Implications for Vaccine Development Article Snippet: .. 2.12 Overexpression and Purification of AdcA-NTD The coding sequence of the extracellular domain of adcA without the secretion signal sequence (amino acids 32-320) from strain MGAS10870 was amplified and cloned into Nde I and Bam HI restriction sites of Sequencing:Article Title: Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity Article Snippet: .. Then, according to the structure of the Article Title: A Critical Role of Zinc Importer AdcABC in Group A Streptococcus-Host Interactions During Infection and Its Implications for Vaccine Development Article Snippet: .. 2.12 Overexpression and Purification of AdcA-NTD The coding sequence of the extracellular domain of adcA without the secretion signal sequence (amino acids 32-320) from strain MGAS10870 was amplified and cloned into Nde I and Bam HI restriction sites of Transformation Assay:Article Title: Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity Article Snippet: .. Cloning and transformation of AMP gene-containing construct The P24 gene was digested with NdeI at 5’ end and EcoRI at the 3´end of the gene for site-directed cloning into the Over Expression:Article Title: A Critical Role of Zinc Importer AdcABC in Group A Streptococcus-Host Interactions During Infection and Its Implications for Vaccine Development Article Snippet: .. 2.12 Overexpression and Purification of AdcA-NTD The coding sequence of the extracellular domain of adcA without the secretion signal sequence (amino acids 32-320) from strain MGAS10870 was amplified and cloned into Nde I and Bam HI restriction sites of Plasmid Preparation:Article Title: Crystal Structure of the Human Fatty Acid Synthase Enoyl-Acyl Carrier Protein-Reductase Domain Complexed with Triclosan Reveals Allosteric Protein-Protein Interface Inhibition * Article Snippet: .. The cDNA was cloned into a Article Title: Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity Article Snippet: .. Then, according to the structure of the Article Title: Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity Article Snippet: .. Article Title: A Critical Role of Zinc Importer AdcABC in Group A Streptococcus-Host Interactions During Infection and Its Implications for Vaccine Development Article Snippet: .. 2.12 Overexpression and Purification of AdcA-NTD The coding sequence of the extracellular domain of adcA without the secretion signal sequence (amino acids 32-320) from strain MGAS10870 was amplified and cloned into Nde I and Bam HI restriction sites of |