ptyb21  (New England Biolabs)


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    Name:
    pTYB21 Vector
    Description:

    Catalog Number:
    N6709
    Price:
    136
    Category:
    Nucleic Acids
    Applications:
    Proteomics & Glycomics
    Size:
    10 µg
    Buy from Supplier


    Structured Review

    New England Biolabs ptyb21
    pTYB21 Vector

    https://www.bioz.com/result/ptyb21/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ptyb21 - by Bioz Stars, 2021-09
    94/100 stars

    Images

    1) Product Images from "Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity"

    Article Title: Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity

    Journal: Iranian Journal of Pharmaceutical Research : IJPR

    doi: 10.22037/ijpr.2019.112397.13734

    The structure of recombinant vector pTYB21 for novel designed AMP (P24). (A) The structure of pTYB21; the important parts are outlined with red box. (B) The sequence of first 5 amino acids at MCS of pTYB21, was added to the N-terminal of P19, indicated in red box
    Figure Legend Snippet: The structure of recombinant vector pTYB21 for novel designed AMP (P24). (A) The structure of pTYB21; the important parts are outlined with red box. (B) The sequence of first 5 amino acids at MCS of pTYB21, was added to the N-terminal of P19, indicated in red box

    Techniques Used: Recombinant, Plasmid Preparation, Sequencing

    2) Product Images from "Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity"

    Article Title: Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity

    Journal: Iranian Journal of Pharmaceutical Research : IJPR

    doi: 10.22037/ijpr.2019.112397.13734

    The structure of recombinant vector pTYB21 for novel designed AMP (P24). (A) The structure of pTYB21; the important parts are outlined with red box. (B) The sequence of first 5 amino acids at MCS of pTYB21, was added to the N-terminal of P19, indicated in red box
    Figure Legend Snippet: The structure of recombinant vector pTYB21 for novel designed AMP (P24). (A) The structure of pTYB21; the important parts are outlined with red box. (B) The sequence of first 5 amino acids at MCS of pTYB21, was added to the N-terminal of P19, indicated in red box

    Techniques Used: Recombinant, Plasmid Preparation, Sequencing

    3) Product Images from "Bile salts and alkaline pH reciprocally modulate the interaction between the periplasmic domains of Vibrio cholerae ToxR and ToxS"

    Article Title: Bile salts and alkaline pH reciprocally modulate the interaction between the periplasmic domains of Vibrio cholerae ToxR and ToxS

    Journal: Molecular microbiology

    doi: 10.1111/mmi.13699

    Cloning and purification of the ToxR and ToxS periplasmic domains. A. Schematic of ToxR (top) and ToxS (bottom) showing domain boundaries. ToxR contains a cytoplasmic DNA binding domain, while ToxS has a 5 amino acid cytoplasmic tail. Both proteins contain a single pass transmembrane domain along with a C-terminal periplasmic domain. DNA encoding the periplasmic domains of ToxR (T199-E294) and ToxS (S25-S173) were cloned in frame into pTYB21, which contains a N-terminal chitin binding domain intein tag. The amino acid sequences of the purified constructs are shown with the periplasmic domain sequences in bold, and the N-terminal five amino acids leftover from the intein tag after cleavage in gray. B. A Colloidal Blue stained 16% SDS-PAGE gel of 1 μg of purified ToxRp and ToxSp.
    Figure Legend Snippet: Cloning and purification of the ToxR and ToxS periplasmic domains. A. Schematic of ToxR (top) and ToxS (bottom) showing domain boundaries. ToxR contains a cytoplasmic DNA binding domain, while ToxS has a 5 amino acid cytoplasmic tail. Both proteins contain a single pass transmembrane domain along with a C-terminal periplasmic domain. DNA encoding the periplasmic domains of ToxR (T199-E294) and ToxS (S25-S173) were cloned in frame into pTYB21, which contains a N-terminal chitin binding domain intein tag. The amino acid sequences of the purified constructs are shown with the periplasmic domain sequences in bold, and the N-terminal five amino acids leftover from the intein tag after cleavage in gray. B. A Colloidal Blue stained 16% SDS-PAGE gel of 1 μg of purified ToxRp and ToxSp.

    Techniques Used: Clone Assay, Purification, Binding Assay, Construct, Staining, SDS Page

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Substrates of the chloroplast small heat shock proteins 22E/F point to thermolability as a regulative switch for heat acclimation in Chlamydomonas reinhardtii
    Article Snippet: .. The 1490-bp PCR product was digested with SapI and NdeI and cloned into pTYB21 (NEB) generating pFW13. ..

    Article Title: Structural and molecular comparison of bacterial and eukaryotic trigger factors
    Article Snippet: .. The TF sequence from E . coli was amplified by PCR from DH5alpha genomic DNA with primers (5′-GGGGCATATGCAAGTTTCAGTTGAAACCAC-3′) and (5′-CCCCGGATCCTTACGCCTGCTGGTTCATC-3′) and cloned with NdeI and BamHI into pTyb21 (NEB) resulting pFW142. ..

    Clone Assay:

    Article Title: Substrates of the chloroplast small heat shock proteins 22E/F point to thermolability as a regulative switch for heat acclimation in Chlamydomonas reinhardtii
    Article Snippet: .. The 1490-bp PCR product was digested with SapI and NdeI and cloned into pTYB21 (NEB) generating pFW13. ..

    Article Title: Molecular Characterization of a Date Palm Vascular Highway 1-Interacting Kinase (PdVIK) under Abiotic Stresses
    Article Snippet: .. Production of Recombinant PdVIK Protein in E. coli The full-length cDNA of PdVIK was amplified using primers containing Nco I and Bam HI sites , and the amplicon was cloned into a pTYB21 vector (New England Biolabs, Ipswich, MA, USA), where its N-terminus was fused in-frame with the Intein tag containing the chitin-binding domain. ..

    Article Title: Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity
    Article Snippet: .. The P24 gene was designed with NdeI restriction site at 5’ end and EcoRI restriction site at the 3´end of the gene for directed cloning into the pTYB21. ..

    Article Title: Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity
    Article Snippet: .. Cloning and transformation of AMP gene-containing construct The P24 gene was digested with NdeI at 5’ end and EcoRI at the 3´end of the gene for site-directed cloning into the pTYB21. ..

    Article Title: The Role of Plastidic Trigger Factor Serving Protein Biogenesis in Green Algae and Land Plants 1The Role of Plastidic Trigger Factor Serving Protein Biogenesis in Green Algae and Land Plants 1 [OPEN]
    Article Snippet: .. Mature cpSRP54 (Cre11.g479750, lacking the predicted chloroplast transit peptide) was cloned from Chlamydomonas cDNA, cut with Nde I and Eco RI, and cloned into pTyb21 (NEB) resulting pFW55. pFW55 was transformed into ER2566 cells, cpSRP54 expression was induced, and the fusion-protein purified via chitin affinity resins according to the manufacturer’s instructions (NEB). ..

    Article Title: Structural and molecular comparison of bacterial and eukaryotic trigger factors
    Article Snippet: .. The TF sequence from E . coli was amplified by PCR from DH5alpha genomic DNA with primers (5′-GGGGCATATGCAAGTTTCAGTTGAAACCAC-3′) and (5′-CCCCGGATCCTTACGCCTGCTGGTTCATC-3′) and cloned with NdeI and BamHI into pTyb21 (NEB) resulting pFW142. ..

    Recombinant:

    Article Title: Molecular Characterization of a Date Palm Vascular Highway 1-Interacting Kinase (PdVIK) under Abiotic Stresses
    Article Snippet: .. Production of Recombinant PdVIK Protein in E. coli The full-length cDNA of PdVIK was amplified using primers containing Nco I and Bam HI sites , and the amplicon was cloned into a pTYB21 vector (New England Biolabs, Ipswich, MA, USA), where its N-terminus was fused in-frame with the Intein tag containing the chitin-binding domain. ..

    Amplification:

    Article Title: Molecular Characterization of a Date Palm Vascular Highway 1-Interacting Kinase (PdVIK) under Abiotic Stresses
    Article Snippet: .. Production of Recombinant PdVIK Protein in E. coli The full-length cDNA of PdVIK was amplified using primers containing Nco I and Bam HI sites , and the amplicon was cloned into a pTYB21 vector (New England Biolabs, Ipswich, MA, USA), where its N-terminus was fused in-frame with the Intein tag containing the chitin-binding domain. ..

    Article Title: Structural and molecular comparison of bacterial and eukaryotic trigger factors
    Article Snippet: .. The TF sequence from E . coli was amplified by PCR from DH5alpha genomic DNA with primers (5′-GGGGCATATGCAAGTTTCAGTTGAAACCAC-3′) and (5′-CCCCGGATCCTTACGCCTGCTGGTTCATC-3′) and cloned with NdeI and BamHI into pTyb21 (NEB) resulting pFW142. ..

    Plasmid Preparation:

    Article Title: Molecular Characterization of a Date Palm Vascular Highway 1-Interacting Kinase (PdVIK) under Abiotic Stresses
    Article Snippet: .. Production of Recombinant PdVIK Protein in E. coli The full-length cDNA of PdVIK was amplified using primers containing Nco I and Bam HI sites , and the amplicon was cloned into a pTYB21 vector (New England Biolabs, Ipswich, MA, USA), where its N-terminus was fused in-frame with the Intein tag containing the chitin-binding domain. ..

    Article Title: Heterotypic Assembly Mechanism Regulates CHIP E3 Ligase Activity
    Article Snippet: .. For the in vitro ubiquitylation reactions, we first generated a pTYB21-UFD-2 expression vector and purified tagless UFD-2 fraction using the intein cleavage site as per the manufacturer protocol (NEB Cat#E6901S). ..

    Article Title: Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity
    Article Snippet: .. Vector pTYB21 (cat# N6709S, ) was supplied from New England Biolab company (UK). ..

    In Vitro:

    Article Title: Heterotypic Assembly Mechanism Regulates CHIP E3 Ligase Activity
    Article Snippet: .. For the in vitro ubiquitylation reactions, we first generated a pTYB21-UFD-2 expression vector and purified tagless UFD-2 fraction using the intein cleavage site as per the manufacturer protocol (NEB Cat#E6901S). ..

    Generated:

    Article Title: Heterotypic Assembly Mechanism Regulates CHIP E3 Ligase Activity
    Article Snippet: .. For the in vitro ubiquitylation reactions, we first generated a pTYB21-UFD-2 expression vector and purified tagless UFD-2 fraction using the intein cleavage site as per the manufacturer protocol (NEB Cat#E6901S). ..

    Expressing:

    Article Title: Heterotypic Assembly Mechanism Regulates CHIP E3 Ligase Activity
    Article Snippet: .. For the in vitro ubiquitylation reactions, we first generated a pTYB21-UFD-2 expression vector and purified tagless UFD-2 fraction using the intein cleavage site as per the manufacturer protocol (NEB Cat#E6901S). ..

    Article Title: The Role of Plastidic Trigger Factor Serving Protein Biogenesis in Green Algae and Land Plants 1The Role of Plastidic Trigger Factor Serving Protein Biogenesis in Green Algae and Land Plants 1 [OPEN]
    Article Snippet: .. Mature cpSRP54 (Cre11.g479750, lacking the predicted chloroplast transit peptide) was cloned from Chlamydomonas cDNA, cut with Nde I and Eco RI, and cloned into pTyb21 (NEB) resulting pFW55. pFW55 was transformed into ER2566 cells, cpSRP54 expression was induced, and the fusion-protein purified via chitin affinity resins according to the manufacturer’s instructions (NEB). ..

    Purification:

    Article Title: Heterotypic Assembly Mechanism Regulates CHIP E3 Ligase Activity
    Article Snippet: .. For the in vitro ubiquitylation reactions, we first generated a pTYB21-UFD-2 expression vector and purified tagless UFD-2 fraction using the intein cleavage site as per the manufacturer protocol (NEB Cat#E6901S). ..

    Article Title: The Role of Plastidic Trigger Factor Serving Protein Biogenesis in Green Algae and Land Plants 1The Role of Plastidic Trigger Factor Serving Protein Biogenesis in Green Algae and Land Plants 1 [OPEN]
    Article Snippet: .. Mature cpSRP54 (Cre11.g479750, lacking the predicted chloroplast transit peptide) was cloned from Chlamydomonas cDNA, cut with Nde I and Eco RI, and cloned into pTyb21 (NEB) resulting pFW55. pFW55 was transformed into ER2566 cells, cpSRP54 expression was induced, and the fusion-protein purified via chitin affinity resins according to the manufacturer’s instructions (NEB). ..

    Transformation Assay:

    Article Title: Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity
    Article Snippet: .. Cloning and transformation of AMP gene-containing construct The P24 gene was digested with NdeI at 5’ end and EcoRI at the 3´end of the gene for site-directed cloning into the pTYB21. ..

    Article Title: The Role of Plastidic Trigger Factor Serving Protein Biogenesis in Green Algae and Land Plants 1The Role of Plastidic Trigger Factor Serving Protein Biogenesis in Green Algae and Land Plants 1 [OPEN]
    Article Snippet: .. Mature cpSRP54 (Cre11.g479750, lacking the predicted chloroplast transit peptide) was cloned from Chlamydomonas cDNA, cut with Nde I and Eco RI, and cloned into pTyb21 (NEB) resulting pFW55. pFW55 was transformed into ER2566 cells, cpSRP54 expression was induced, and the fusion-protein purified via chitin affinity resins according to the manufacturer’s instructions (NEB). ..

    Construct:

    Article Title: Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity
    Article Snippet: .. Cloning and transformation of AMP gene-containing construct The P24 gene was digested with NdeI at 5’ end and EcoRI at the 3´end of the gene for site-directed cloning into the pTYB21. ..

    Sequencing:

    Article Title: Structural and molecular comparison of bacterial and eukaryotic trigger factors
    Article Snippet: .. The TF sequence from E . coli was amplified by PCR from DH5alpha genomic DNA with primers (5′-GGGGCATATGCAAGTTTCAGTTGAAACCAC-3′) and (5′-CCCCGGATCCTTACGCCTGCTGGTTCATC-3′) and cloned with NdeI and BamHI into pTyb21 (NEB) resulting pFW142. ..

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  • 94
    New England Biolabs ptyb21
    The structure of recombinant vector <t>pTYB21</t> for novel designed AMP (P24). (A) The structure of pTYB21; the important parts are outlined with red box. (B) The sequence of first 5 amino acids at MCS of pTYB21, was added to the N-terminal of P19, indicated in red box
    Ptyb21, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ptyb21/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ptyb21 - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    93
    New England Biolabs vector ptyb21
    The structure of recombinant vector <t>pTYB21</t> for novel designed AMP (P24). (A) The structure of pTYB21; the important parts are outlined with red box. (B) The sequence of first 5 amino acids at MCS of pTYB21, was added to the N-terminal of P19, indicated in red box
    Vector Ptyb21, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vector ptyb21/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vector ptyb21 - by Bioz Stars, 2021-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    The structure of recombinant vector pTYB21 for novel designed AMP (P24). (A) The structure of pTYB21; the important parts are outlined with red box. (B) The sequence of first 5 amino acids at MCS of pTYB21, was added to the N-terminal of P19, indicated in red box

    Journal: Iranian Journal of Pharmaceutical Research : IJPR

    Article Title: Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity

    doi: 10.22037/ijpr.2019.112397.13734

    Figure Lengend Snippet: The structure of recombinant vector pTYB21 for novel designed AMP (P24). (A) The structure of pTYB21; the important parts are outlined with red box. (B) The sequence of first 5 amino acids at MCS of pTYB21, was added to the N-terminal of P19, indicated in red box

    Article Snippet: Cloning and transformation of AMP gene-containing construct The P24 gene was digested with NdeI at 5’ end and EcoRI at the 3´end of the gene for site-directed cloning into the pTYB21.

    Techniques: Recombinant, Plasmid Preparation, Sequencing

    Cloning and purification of the ToxR and ToxS periplasmic domains. A. Schematic of ToxR (top) and ToxS (bottom) showing domain boundaries. ToxR contains a cytoplasmic DNA binding domain, while ToxS has a 5 amino acid cytoplasmic tail. Both proteins contain a single pass transmembrane domain along with a C-terminal periplasmic domain. DNA encoding the periplasmic domains of ToxR (T199-E294) and ToxS (S25-S173) were cloned in frame into pTYB21, which contains a N-terminal chitin binding domain intein tag. The amino acid sequences of the purified constructs are shown with the periplasmic domain sequences in bold, and the N-terminal five amino acids leftover from the intein tag after cleavage in gray. B. A Colloidal Blue stained 16% SDS-PAGE gel of 1 μg of purified ToxRp and ToxSp.

    Journal: Molecular microbiology

    Article Title: Bile salts and alkaline pH reciprocally modulate the interaction between the periplasmic domains of Vibrio cholerae ToxR and ToxS

    doi: 10.1111/mmi.13699

    Figure Lengend Snippet: Cloning and purification of the ToxR and ToxS periplasmic domains. A. Schematic of ToxR (top) and ToxS (bottom) showing domain boundaries. ToxR contains a cytoplasmic DNA binding domain, while ToxS has a 5 amino acid cytoplasmic tail. Both proteins contain a single pass transmembrane domain along with a C-terminal periplasmic domain. DNA encoding the periplasmic domains of ToxR (T199-E294) and ToxS (S25-S173) were cloned in frame into pTYB21, which contains a N-terminal chitin binding domain intein tag. The amino acid sequences of the purified constructs are shown with the periplasmic domain sequences in bold, and the N-terminal five amino acids leftover from the intein tag after cleavage in gray. B. A Colloidal Blue stained 16% SDS-PAGE gel of 1 μg of purified ToxRp and ToxSp.

    Article Snippet: DNA corresponding to the amino acid sequences of V. cholerae El Tor N16961 ToxR (VC0984) T199-E294 and ToxS (VC0983) S25-S173 were ordered from DNA2.0 and inserted into pTYB21 (NEB).

    Techniques: Clone Assay, Purification, Binding Assay, Construct, Staining, SDS Page

    The structure of recombinant vector pTYB21 for novel designed AMP (P24). (A) The structure of pTYB21; the important parts are outlined with red box. (B) The sequence of first 5 amino acids at MCS of pTYB21, was added to the N-terminal of P19, indicated in red box

    Journal: Iranian Journal of Pharmaceutical Research : IJPR

    Article Title: Effect of Amino Acid Substitutions on Biological Activity of Antimicrobial Peptide: Design, Recombinant Production, and Biological Activity

    doi: 10.22037/ijpr.2019.112397.13734

    Figure Lengend Snippet: The structure of recombinant vector pTYB21 for novel designed AMP (P24). (A) The structure of pTYB21; the important parts are outlined with red box. (B) The sequence of first 5 amino acids at MCS of pTYB21, was added to the N-terminal of P19, indicated in red box

    Article Snippet: Then, according to the structure of the vector pTYB21, some modifications were applied in to the designed AMP (P19) and five amino acid residues (GRAHM) were added to the N-terminal of P19, which resulted in 24 amino acid sequence of P24.

    Techniques: Recombinant, Plasmid Preparation, Sequencing