fast dna ladder  (New England Biolabs)


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    Name:
    Fast DNA Ladder
    Description:
    Fast DNA Ladder 50 200 gel lanes
    Catalog Number:
    n3238s
    Price:
    61
    Size:
    50 gel lanes
    Category:
    DNA Ladders
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    Structured Review

    New England Biolabs fast dna ladder
    Fast DNA Ladder
    Fast DNA Ladder 50 200 gel lanes
    https://www.bioz.com/result/fast dna ladder/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fast dna ladder - by Bioz Stars, 2020-02
    93/100 stars

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    Related Articles

    DNA Extraction:

    Article Title: Draft genome sequence of Xylaria sp., the causal agent of taproot decline of soybean in the southern United States
    Article Snippet: Paragraph title: Genomic DNA extraction and sequencing ... A 400 bp library was prepared from genomic DNA with the NEBNext Fast DNA Fragmentation and Library Preparation Kit (New England Biolabs, Ipswich, NE, USA) following the manufacturer's instructions.

    Article Title: Calm before the Storm: A Glimpse into the Secondary Metabolism of Aspergillus welwitschiae, the Etiologic Agent of the Sisal Bole Rot
    Article Snippet: Genome Sequencing and Assembly The mycelium of isolate CCMB 674 was grown on potato dextrose agar medium (PDA; Sigma-Aldrich, St. Louis, MS, USA) and incubated at 25 °C for five days, after which it was scrapped for genomic DNA extraction, performed with FastDNATM for Soil kit (MP Biomedicals, Santa Ana CA, USA). .. A 450 bp library was prepared with NEBNext Fast DNA Fragmentation and Library Preparation Kit (New England Biolabs, Ipswich, NE, USA) following the instructions of the manufacturer.

    Centrifugation:

    Article Title: Draft genome sequence of Xylaria sp., the causal agent of taproot decline of soybean in the southern United States
    Article Snippet: Fungal tissue was collected by centrifugation at 4000 rpm for 5 min and ground with liquid nitrogen. .. A 400 bp library was prepared from genomic DNA with the NEBNext Fast DNA Fragmentation and Library Preparation Kit (New England Biolabs, Ipswich, NE, USA) following the manufacturer's instructions.

    Amplification:

    Article Title: Evaluation of multiple approaches to identify genome-wide polymorphisms in closely related genotypes of sweet cherry (Prunus avium L.)
    Article Snippet: .. Following TRAP amplification and PCR cleanup, the reduced representation sample library was prepared using the NEBNext® Fast DNA Library Prep Set as per the manufacturer's instructions with the following modifications. .. TRAP PCR products from each reaction were sheared with NEB Next Fragmentase.

    Agarose Gel Electrophoresis:

    Article Title: NCR1 Expression Identifies Canine Natural Killer Cell Subsets with Phenotypic Similarity to Human Natural Killer Cells
    Article Snippet: .. Semi-quantitative RT-PCR reactions were run on five different donors with 100 ng cDNA per sample and 6× DNA loading dye, and products were verified on a 1.5% agarose gel with TAE buffer and GelRed Nucleic Acid Stain (Phenix, RGB-4103) and 100 bp DNA Ladder (New England Biolabs, N3238S). .. Gels were imaged on BioRad ChemiDoc Touch Imaging System (BioRad, USA) for Faint Bands and analyzed using Image Lab 5.2.1.

    Article Title: Draft genome sequence of Xylaria sp., the causal agent of taproot decline of soybean in the southern United States
    Article Snippet: The quality and quantity of the genomic DNA were assessed by agarose gel electrophoresis and spectrophotometric analysis, respectively. .. A 400 bp library was prepared from genomic DNA with the NEBNext Fast DNA Fragmentation and Library Preparation Kit (New England Biolabs, Ipswich, NE, USA) following the manufacturer's instructions.

    Article Title: Calm before the Storm: A Glimpse into the Secondary Metabolism of Aspergillus welwitschiae, the Etiologic Agent of the Sisal Bole Rot
    Article Snippet: Genomic DNA quality and quantity were assessed by agarose gel electrophoresis and fluorometric analysis, respectively. .. A 450 bp library was prepared with NEBNext Fast DNA Fragmentation and Library Preparation Kit (New England Biolabs, Ipswich, NE, USA) following the instructions of the manufacturer.

    Synthesized:

    Article Title: NCR1 Expression Identifies Canine Natural Killer Cell Subsets with Phenotypic Similarity to Human Natural Killer Cells
    Article Snippet: RT-PCR/qPCR RNA was isolated from canine expanded cells using RNAeasy Kit, QiaShredder Columns, and RNAase-Free DNase Set (all Qiagen, 74104, 79654, 79254), and cDNA was synthesized using Omniscript RT Kit (Qiagen, 20511) with RNase inhibitor (New England Biolabs, M0307S) and Oligo(dt)20 (ThermoFisher Scientific, 18418-020). .. Semi-quantitative RT-PCR reactions were run on five different donors with 100 ng cDNA per sample and 6× DNA loading dye, and products were verified on a 1.5% agarose gel with TAE buffer and GelRed Nucleic Acid Stain (Phenix, RGB-4103) and 100 bp DNA Ladder (New England Biolabs, N3238S).

    Isolation:

    Article Title: NCR1 Expression Identifies Canine Natural Killer Cell Subsets with Phenotypic Similarity to Human Natural Killer Cells
    Article Snippet: RT-PCR/qPCR RNA was isolated from canine expanded cells using RNAeasy Kit, QiaShredder Columns, and RNAase-Free DNase Set (all Qiagen, 74104, 79654, 79254), and cDNA was synthesized using Omniscript RT Kit (Qiagen, 20511) with RNase inhibitor (New England Biolabs, M0307S) and Oligo(dt)20 (ThermoFisher Scientific, 18418-020). .. Semi-quantitative RT-PCR reactions were run on five different donors with 100 ng cDNA per sample and 6× DNA loading dye, and products were verified on a 1.5% agarose gel with TAE buffer and GelRed Nucleic Acid Stain (Phenix, RGB-4103) and 100 bp DNA Ladder (New England Biolabs, N3238S).

    Next-Generation Sequencing:

    Article Title: period-1 encodes an ATP-dependent RNA helicase that influences nutritional compensation of the Neurospora circadian clock
    Article Snippet: Using the NEBNext fast DNA fragmentation and library prep kit, 100 ng of DNA was prepared for sequencing by generating adaptor-ligated DNA compatible with ION Torrent chemistry (E6285; NEB). .. After XP bead clean up, libraries were quantified with Qubit, and then the DNA profile was analyzed with the Fragment Analyzer (High Sensitivity NGS kit).

    Article Title: Effect of Selected Stilbenoids on Human Fecal Microbiota
    Article Snippet: Paragraph title: 3.4.3. Semi-conductor Based Next Generation Sequencing ... 200 ng of DNA from each sample was used to prepare sequencing libraries by NEBNext® Fast DNA Library Prep Set kit (New England Biolabs, Ipswich, MA, USA) according to manufacturer’s protocol.

    Article Title: Evaluation of multiple approaches to identify genome-wide polymorphisms in closely related genotypes of sweet cherry (Prunus avium L.)
    Article Snippet: Amplification was followed by generation of NGS sequence data from the products (Ion Torrent PGM, Thermo Fisher Scientific, Inc., Waltham, MA). .. Following TRAP amplification and PCR cleanup, the reduced representation sample library was prepared using the NEBNext® Fast DNA Library Prep Set as per the manufacturer's instructions with the following modifications.

    Generated:

    Article Title: Evaluation of multiple approaches to identify genome-wide polymorphisms in closely related genotypes of sweet cherry (Prunus avium L.)
    Article Snippet: The short read sequence data generated from TRAPseq was submitted to NCBI under the following accession numbers: SRS1706064 - Glory_Trapseq and SRS1706056 - Staccato_Trapseq. .. Following TRAP amplification and PCR cleanup, the reduced representation sample library was prepared using the NEBNext® Fast DNA Library Prep Set as per the manufacturer's instructions with the following modifications.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: NCR1 Expression Identifies Canine Natural Killer Cell Subsets with Phenotypic Similarity to Human Natural Killer Cells
    Article Snippet: .. Semi-quantitative RT-PCR reactions were run on five different donors with 100 ng cDNA per sample and 6× DNA loading dye, and products were verified on a 1.5% agarose gel with TAE buffer and GelRed Nucleic Acid Stain (Phenix, RGB-4103) and 100 bp DNA Ladder (New England Biolabs, N3238S). .. Gels were imaged on BioRad ChemiDoc Touch Imaging System (BioRad, USA) for Faint Bands and analyzed using Image Lab 5.2.1.

    SYBR Green Assay:

    Article Title: NCR1 Expression Identifies Canine Natural Killer Cell Subsets with Phenotypic Similarity to Human Natural Killer Cells
    Article Snippet: RT-PCR and qPCR reactions were performed on Roche 480 (Roche, USA) using Power Up Sybr Green Master Mix (ThermoFisher; A25742) or TaqMan Universal PCR Mastermix for qPCR for CD16 (ThermoFisher Scientific, 4304437) for 40 cycles. .. Semi-quantitative RT-PCR reactions were run on five different donors with 100 ng cDNA per sample and 6× DNA loading dye, and products were verified on a 1.5% agarose gel with TAE buffer and GelRed Nucleic Acid Stain (Phenix, RGB-4103) and 100 bp DNA Ladder (New England Biolabs, N3238S).

    Activation Assay:

    Article Title: NCR1 Expression Identifies Canine Natural Killer Cell Subsets with Phenotypic Similarity to Human Natural Killer Cells
    Article Snippet: Semi-quantitative RT-PCR reactions were run on five different donors with 100 ng cDNA per sample and 6× DNA loading dye, and products were verified on a 1.5% agarose gel with TAE buffer and GelRed Nucleic Acid Stain (Phenix, RGB-4103) and 100 bp DNA Ladder (New England Biolabs, N3238S). .. PCR cycling conditions were as followings: UDG activation: 50°C – 2 min, Polymerase Activation: 95°C – 2 min, Denature: 95°C – 15 s, B-actin, NKp46, NKp30, Granzyme B, and Perforin – Anneal/Extend: 62°C – 1 min; B-actin, Ly49, and CD16 – Anneal: 54°C – 15 s, Extension: 72°C – 1 min; B-actin, DNAM-1, NKp30, NKp44, NKG2D, and NKp80 at the same conditions as Ly49 and CD16 with the exception of Annealing at 59°C.

    Incubation:

    Article Title: Calm before the Storm: A Glimpse into the Secondary Metabolism of Aspergillus welwitschiae, the Etiologic Agent of the Sisal Bole Rot
    Article Snippet: Genome Sequencing and Assembly The mycelium of isolate CCMB 674 was grown on potato dextrose agar medium (PDA; Sigma-Aldrich, St. Louis, MS, USA) and incubated at 25 °C for five days, after which it was scrapped for genomic DNA extraction, performed with FastDNATM for Soil kit (MP Biomedicals, Santa Ana CA, USA). .. A 450 bp library was prepared with NEBNext Fast DNA Fragmentation and Library Preparation Kit (New England Biolabs, Ipswich, NE, USA) following the instructions of the manufacturer.

    Polymerase Chain Reaction:

    Article Title: NCR1 Expression Identifies Canine Natural Killer Cell Subsets with Phenotypic Similarity to Human Natural Killer Cells
    Article Snippet: RT-PCR and qPCR reactions were performed on Roche 480 (Roche, USA) using Power Up Sybr Green Master Mix (ThermoFisher; A25742) or TaqMan Universal PCR Mastermix for qPCR for CD16 (ThermoFisher Scientific, 4304437) for 40 cycles. .. Semi-quantitative RT-PCR reactions were run on five different donors with 100 ng cDNA per sample and 6× DNA loading dye, and products were verified on a 1.5% agarose gel with TAE buffer and GelRed Nucleic Acid Stain (Phenix, RGB-4103) and 100 bp DNA Ladder (New England Biolabs, N3238S).

    Article Title: Effect of Selected Stilbenoids on Human Fecal Microbiota
    Article Snippet: Semi-conductor Based Next Generation Sequencing Obtained PCR products were used to prepare libraries for diversity analyses by next generation sequencing (NGS) approach on Personal Genome Machine (Life Technologies, Carlsbad, CA, USA) according to Milani et al. [ ]. .. 200 ng of DNA from each sample was used to prepare sequencing libraries by NEBNext® Fast DNA Library Prep Set kit (New England Biolabs, Ipswich, MA, USA) according to manufacturer’s protocol.

    Article Title: Evaluation of multiple approaches to identify genome-wide polymorphisms in closely related genotypes of sweet cherry (Prunus avium L.)
    Article Snippet: .. Following TRAP amplification and PCR cleanup, the reduced representation sample library was prepared using the NEBNext® Fast DNA Library Prep Set as per the manufacturer's instructions with the following modifications. .. TRAP PCR products from each reaction were sheared with NEB Next Fragmentase.

    Imaging:

    Article Title: NCR1 Expression Identifies Canine Natural Killer Cell Subsets with Phenotypic Similarity to Human Natural Killer Cells
    Article Snippet: Semi-quantitative RT-PCR reactions were run on five different donors with 100 ng cDNA per sample and 6× DNA loading dye, and products were verified on a 1.5% agarose gel with TAE buffer and GelRed Nucleic Acid Stain (Phenix, RGB-4103) and 100 bp DNA Ladder (New England Biolabs, N3238S). .. Gels were imaged on BioRad ChemiDoc Touch Imaging System (BioRad, USA) for Faint Bands and analyzed using Image Lab 5.2.1.

    Mass Spectrometry:

    Article Title: Calm before the Storm: A Glimpse into the Secondary Metabolism of Aspergillus welwitschiae, the Etiologic Agent of the Sisal Bole Rot
    Article Snippet: Genome Sequencing and Assembly The mycelium of isolate CCMB 674 was grown on potato dextrose agar medium (PDA; Sigma-Aldrich, St. Louis, MS, USA) and incubated at 25 °C for five days, after which it was scrapped for genomic DNA extraction, performed with FastDNATM for Soil kit (MP Biomedicals, Santa Ana CA, USA). .. A 450 bp library was prepared with NEBNext Fast DNA Fragmentation and Library Preparation Kit (New England Biolabs, Ipswich, NE, USA) following the instructions of the manufacturer.

    Sequencing:

    Article Title: Draft genome sequence of Xylaria sp., the causal agent of taproot decline of soybean in the southern United States
    Article Snippet: Paragraph title: Genomic DNA extraction and sequencing ... A 400 bp library was prepared from genomic DNA with the NEBNext Fast DNA Fragmentation and Library Preparation Kit (New England Biolabs, Ipswich, NE, USA) following the manufacturer's instructions.

    Article Title: Cocirculation of Two Distinct Genetic and Antigenic Lineages of Proposed Influenza D Virus in Cattle
    Article Snippet: .. In brief, sequencing libraries were prepared from full-genome amplicons using a NEBNext Fast DNA kit for Ion Torrent. .. D/OK (accession numbers to ) was used as the template to assemble the contigs using the SegMan NGen module from DNAStar.

    Article Title: period-1 encodes an ATP-dependent RNA helicase that influences nutritional compensation of the Neurospora circadian clock
    Article Snippet: .. Using the NEBNext fast DNA fragmentation and library prep kit, 100 ng of DNA was prepared for sequencing by generating adaptor-ligated DNA compatible with ION Torrent chemistry (E6285; NEB). ..

    Article Title: Effect of Selected Stilbenoids on Human Fecal Microbiota
    Article Snippet: .. 200 ng of DNA from each sample was used to prepare sequencing libraries by NEBNext® Fast DNA Library Prep Set kit (New England Biolabs, Ipswich, MA, USA) according to manufacturer’s protocol. .. The Ion Xpress Barcode adapters (Thermo Fisher Scientific, Waltham, MA, USA) were used to label each sample.

    Article Title: Evaluation of multiple approaches to identify genome-wide polymorphisms in closely related genotypes of sweet cherry (Prunus avium L.)
    Article Snippet: The short read sequence data generated from TRAPseq was submitted to NCBI under the following accession numbers: SRS1706064 - Glory_Trapseq and SRS1706056 - Staccato_Trapseq. .. Following TRAP amplification and PCR cleanup, the reduced representation sample library was prepared using the NEBNext® Fast DNA Library Prep Set as per the manufacturer's instructions with the following modifications.

    Article Title: Calm before the Storm: A Glimpse into the Secondary Metabolism of Aspergillus welwitschiae, the Etiologic Agent of the Sisal Bole Rot
    Article Snippet: Paragraph title: 4.2. Genome Sequencing and Assembly ... A 450 bp library was prepared with NEBNext Fast DNA Fragmentation and Library Preparation Kit (New England Biolabs, Ipswich, NE, USA) following the instructions of the manufacturer.

    Staining:

    Article Title: NCR1 Expression Identifies Canine Natural Killer Cell Subsets with Phenotypic Similarity to Human Natural Killer Cells
    Article Snippet: .. Semi-quantitative RT-PCR reactions were run on five different donors with 100 ng cDNA per sample and 6× DNA loading dye, and products were verified on a 1.5% agarose gel with TAE buffer and GelRed Nucleic Acid Stain (Phenix, RGB-4103) and 100 bp DNA Ladder (New England Biolabs, N3238S). .. Gels were imaged on BioRad ChemiDoc Touch Imaging System (BioRad, USA) for Faint Bands and analyzed using Image Lab 5.2.1.

    Purification:

    Article Title: Effect of Selected Stilbenoids on Human Fecal Microbiota
    Article Snippet: 200 ng of DNA from each sample was used to prepare sequencing libraries by NEBNext® Fast DNA Library Prep Set kit (New England Biolabs, Ipswich, MA, USA) according to manufacturer’s protocol. .. The adaptor ligated libraries were purified and simultaneously size-selected using AMPure XP bead sizing (Beckman Coulter, Brea, CA, USA).

    Article Title: Evaluation of multiple approaches to identify genome-wide polymorphisms in closely related genotypes of sweet cherry (Prunus avium L.)
    Article Snippet: Following TRAP amplification and PCR cleanup, the reduced representation sample library was prepared using the NEBNext® Fast DNA Library Prep Set as per the manufacturer's instructions with the following modifications. .. Complementary, custom adaptors were then annealed to the sheared DNA, the annealed product was purified and extracted according the NEBNext FastDNA Library Prep protocol.

    Chromatin Immunoprecipitation:

    Article Title: Draft genome sequence of Xylaria sp., the causal agent of taproot decline of soybean in the southern United States
    Article Snippet: A 400 bp library was prepared from genomic DNA with the NEBNext Fast DNA Fragmentation and Library Preparation Kit (New England Biolabs, Ipswich, NE, USA) following the manufacturer's instructions. .. Whole genome sequencing was performed at the University of Arkansas, Fayetteville, AR, USA, with an Ion Torrent Personal Genome Machine (PGM) (Thermo Fisher Scientific, Waltham, MA, USA) and a 318 V2 chip kit.

    Software:

    Article Title: Cocirculation of Two Distinct Genetic and Antigenic Lineages of Proposed Influenza D Virus in Cattle
    Article Snippet: In brief, sequencing libraries were prepared from full-genome amplicons using a NEBNext Fast DNA kit for Ion Torrent. .. Phylogenetic analysis was performed using MEGA6.0 software using the maximum-likelihood algorithm with 1,000 bootstrap replicates to verify tree topology.

    Article Title: Calm before the Storm: A Glimpse into the Secondary Metabolism of Aspergillus welwitschiae, the Etiologic Agent of the Sisal Bole Rot
    Article Snippet: A 450 bp library was prepared with NEBNext Fast DNA Fragmentation and Library Preparation Kit (New England Biolabs, Ipswich, NE, USA) following the instructions of the manufacturer. .. Raw reads were trimmed (Phred score > 20) with BBDuk, and normalized with BBNorm, with both forms of software being components of the BBTools v.36.86 software kit [ ].

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  • 93
    New England Biolabs fast dna ladder
    Fast Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fast dna ladder/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fast dna ladder - by Bioz Stars, 2020-02
    93/100 stars
      Buy from Supplier

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