pcr  (New England Biolabs)


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  • 95
    Name:
    PCR Marker
    Description:
    PCR Marker 500 gel lanes
    Catalog Number:
    n3234l
    Price:
    272
    Size:
    500 gel lanes
    Category:
    PCR Molecular Weight Markers
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    Structured Review

    New England Biolabs pcr
    PCR Marker
    PCR Marker 500 gel lanes
    https://www.bioz.com/result/pcr/product/New England Biolabs
    Average 95 stars, based on 12229 article reviews
    Price from $9.99 to $1999.99
    pcr - by Bioz Stars, 2020-02
    95/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Uptake of denatured collagen into hepatic stellate cells: evidence for the involvement of urokinase plasminogen activator receptor-associated protein/Endo180
    Article Snippet: Routine PCR was performed using DyNAzyme (Finnzymes, Espoo, Finland) according to the manufacturer's instructions and performed at the following conditions: 94 °C for 5 min, 35 cycles each 30 s at 94 °C, 60 °C for 30 s and 72 °C for 30 s. The PCR products were cloned into pGEM-T Easy Vector (Promega) and sequenced (GATC-Biotech, Konstanz, Germany). .. PCR marker was provided by New England Biolabs (Beverly, MA, U.S.A.).

    Centrifugation:

    Article Title: Collection of cell-free DNA for genomic analysis of solid tumors in a clinical laboratory setting
    Article Snippet: Clarified plasma was prepared from 10 mL tubes by centrifugation at 2000 x g for 10 min; transfer of the initial plasma fraction to a conical 15 mL centrifuge tube and a second 2000 x g spin for 10 min; and decanting of the clarified plasma, carefully avoiding the residual pellet material. .. Size analysis was performed using gel electrophoresis on 2% agarose gels with PCR markers as size standards (New England Biolabs, Ipswich, MA).

    Synthesized:

    Article Title: Uptake of denatured collagen into hepatic stellate cells: evidence for the involvement of urokinase plasminogen activator receptor-associated protein/Endo180
    Article Snippet: First-strand cDNA was synthesized from 5 μg of total RNA using SuperScript RT (Invitrogen). .. PCR marker was provided by New England Biolabs (Beverly, MA, U.S.A.).

    Quantitative RT-PCR:

    Article Title: Identification of genes for normalization of real-time RT-PCR data in breast carcinomas
    Article Snippet: ▪ PCR A PCR was performed with the same program as RT-qPCR for the 4-group reference genes for ER+ IBC with cDNA from one patient (no. 9) and the MCF-7 cell line. .. The products were run on a 3% NuSieve GTG agarose gel (Cambrex Bioscience Rockland Inc., Rockland, ME) with 20 μg EtBr (Sigma, St. Louis, MO) in 1× TBE buffer (Sigma) at 5 V/5 min followed by 100 V/45 min (BioRad, Hercules, CA) along with a PCR marker (New England BioLabs Inc., Ipswich, MA).

    Electrophoresis:

    Article Title: Diepoxybutane Interstrand Cross-Links Induce DNA Bending
    Article Snippet: Paragraph title: 2.3 Ligation and Electrophoresis ... PCR marker (New England Biolabs) was used as a size reference (765 bp, 500 bp, 300 bp, 150 bp, and 50 bp, with the 300-bp marker resolving as two bands).

    Article Title: The association between XRCC1 polymorphism and laryngeal cancer susceptibility in different ethnic groups in Xinjiang, China
    Article Snippet: A BGPower 300 electrophoresis meter was purchased from Beijing Biotechnology Co., LTD. .. A PCR Marker was purchased from NEW ENGLAND Biolabs.

    Quantitation Assay:

    Article Title: Collection of cell-free DNA for genomic analysis of solid tumors in a clinical laboratory setting
    Article Snippet: The yield of double-strand DNA was quantified using a Qubit fluorometer (Thermo Fisher, Waltham, MA) and the corresponding hsDNA quantitation kit. .. Size analysis was performed using gel electrophoresis on 2% agarose gels with PCR markers as size standards (New England Biolabs, Ipswich, MA).

    Expressing:

    Article Title: Uptake of denatured collagen into hepatic stellate cells: evidence for the involvement of urokinase plasminogen activator receptor-associated protein/Endo180
    Article Snippet: Paragraph title: Analysis of gene expression by RT (reverse transcriptase)–PCR ... PCR marker was provided by New England Biolabs (Beverly, MA, U.S.A.).

    Derivative Assay:

    Article Title: Diepoxybutane Interstrand Cross-Links Induce DNA Bending
    Article Snippet: PCR marker (New England Biolabs) was used as a size reference (765 bp, 500 bp, 300 bp, 150 bp, and 50 bp, with the 300-bp marker resolving as two bands). .. We then determined the angle of absolute curvature per helix turn, C, through the empirical relationship derived by Koo and Crothers [ , ]: C =\u00a0[(RL \u22121)/(9.6\u00d710\u22125 \u2022Lr 2 \u22120.47)]1/2 \u00a0\u00d7\u00a0(19.75\u00a0\u00b1\u00a02.75\u00b0/turn) We corrected bend angles per cross-link to a helical repeat of 10.5 bp per turn.

    Article Title: Transposable Element Insertion and Epigenetic Modification Cause the Multiallelic Variation in the Expression of FAE1 in Sinapis alba
    Article Snippet: The cosegregation of the marker specific for the allele e and zero erucic content was studied in 96 F2 plants derived from the cross Y514 ( ee ) × Y517 ( E1 E1 ), while the cosegregation of the marker specific for the allele E2 with medium erucic content was studied in 70 F2 plants of the cross Y496 ( E2 E2 ) × Y517 ( E1 E1 ). .. Each PCR (20 µL) contained 1× PCR standard buffer (NEB), 200 µM each deoxynucleotide triphosphate, 0.1 µM of each forward and reverse primer, 1 unit of Taq polymerase (NEB), and 50 ng of genomic DNA.

    Ligation:

    Article Title: Diepoxybutane Interstrand Cross-Links Induce DNA Bending
    Article Snippet: Paragraph title: 2.3 Ligation and Electrophoresis ... PCR marker (New England Biolabs) was used as a size reference (765 bp, 500 bp, 300 bp, 150 bp, and 50 bp, with the 300-bp marker resolving as two bands).

    Introduce:

    Article Title: Rapid GAL Gene Switch of Saccharomyces cerevisiae Depends on Nuclear Gal3, Not Nucleocytoplasmic Trafficking of Gal3 and Gal80
    Article Snippet: To introduce NES and mNES (L326 , I328 ) into , the region of -encoding residues (AA 316–357) was isolated from pGEM-T (AA 316–357) and pGEM-T (AA 316–357) ( ) by PCR with primers OE167 (5′-CTCGTCGACGTCGAAAAGGGAAATTTACCTGA-3′) and OE168 (5′-CTCGTCGACGTCCTCTTCAAAATCGTCAACCT-3′). .. The P -2GFP reporter plasmid, pOE33, was constructed by PCR-based and other molecular methods in pRS416 (New England Biolabs).

    Polymerase Chain Reaction:

    Article Title: Uptake of denatured collagen into hepatic stellate cells: evidence for the involvement of urokinase plasminogen activator receptor-associated protein/Endo180
    Article Snippet: .. PCR marker was provided by New England Biolabs (Beverly, MA, U.S.A.). ..

    Article Title: Diepoxybutane Interstrand Cross-Links Induce DNA Bending
    Article Snippet: .. PCR marker (New England Biolabs) was used as a size reference (765 bp, 500 bp, 300 bp, 150 bp, and 50 bp, with the 300-bp marker resolving as two bands). ..

    Article Title: Identification of genes for normalization of real-time RT-PCR data in breast carcinomas
    Article Snippet: .. The products were run on a 3% NuSieve GTG agarose gel (Cambrex Bioscience Rockland Inc., Rockland, ME) with 20 μg EtBr (Sigma, St. Louis, MO) in 1× TBE buffer (Sigma) at 5 V/5 min followed by 100 V/45 min (BioRad, Hercules, CA) along with a PCR marker (New England BioLabs Inc., Ipswich, MA). .. ▪ Immunohistochemistry (IHC) The NCL-ER-6F11 antibody (Novocastra Ltd., Newcastle, UK) was used for ER detection.

    Article Title: Collection of cell-free DNA for genomic analysis of solid tumors in a clinical laboratory setting
    Article Snippet: .. Size analysis was performed using gel electrophoresis on 2% agarose gels with PCR markers as size standards (New England Biolabs, Ipswich, MA). .. The spike-in DNA used in this study was structural multiplex cfDNA reference standard, product HD786, from Horizon (Cambridge, UK).

    Article Title: The association between XRCC1 polymorphism and laryngeal cancer susceptibility in different ethnic groups in Xinjiang, China
    Article Snippet: .. A PCR Marker was purchased from NEW ENGLAND Biolabs. .. BIOWEST agarose was purchased from Shanghai Xiayi Industrial Co., LTD.

    Article Title: Transposable Element Insertion and Epigenetic Modification Cause the Multiallelic Variation in the Expression of FAE1 in Sinapis alba
    Article Snippet: .. Each PCR (20 µL) contained 1× PCR standard buffer (NEB), 200 µM each deoxynucleotide triphosphate, 0.1 µM of each forward and reverse primer, 1 unit of Taq polymerase (NEB), and 50 ng of genomic DNA. .. PCR was performed with an initial denaturation at 94°C for 3 min followed by 30 cycles of 30 s at 94°C, 30 s at 58°C, and 1 min at 72°C, followed by a final extension cycle of 72°C for 7 min.

    Article Title: A patterned anisotropic nanofluidic sieving structure for continuous-flow separation of DNA and proteins
    Article Snippet: .. The PCR marker as well as λ DNA–Hind III digest (New England BioLabs, Beverly, MA) were labeled with the intercalating fluorescence dye YOYO-1 (Molecular Probes, Eugene, OR) in TBE 5× buffer. .. The dye to DNA base pair ratio was about 1:2 and the final DNA concentration was about 42.18 μg/ml (PCR) and 104 μg/ml (λ DNA–Hind III digest).

    Article Title: Rapid GAL Gene Switch of Saccharomyces cerevisiae Depends on Nuclear Gal3, Not Nucleocytoplasmic Trafficking of Gal3 and Gal80
    Article Snippet: .. The P -2GFP reporter plasmid, pOE33, was constructed by PCR-based and other molecular methods in pRS416 (New England Biolabs). ..

    Nucleic Acid Electrophoresis:

    Article Title: Collection of cell-free DNA for genomic analysis of solid tumors in a clinical laboratory setting
    Article Snippet: .. Size analysis was performed using gel electrophoresis on 2% agarose gels with PCR markers as size standards (New England Biolabs, Ipswich, MA). .. The spike-in DNA used in this study was structural multiplex cfDNA reference standard, product HD786, from Horizon (Cambridge, UK).

    Fluorescence:

    Article Title: A patterned anisotropic nanofluidic sieving structure for continuous-flow separation of DNA and proteins
    Article Snippet: .. The PCR marker as well as λ DNA–Hind III digest (New England BioLabs, Beverly, MA) were labeled with the intercalating fluorescence dye YOYO-1 (Molecular Probes, Eugene, OR) in TBE 5× buffer. .. The dye to DNA base pair ratio was about 1:2 and the final DNA concentration was about 42.18 μg/ml (PCR) and 104 μg/ml (λ DNA–Hind III digest).

    Mutagenesis:

    Article Title: Rapid GAL Gene Switch of Saccharomyces cerevisiae Depends on Nuclear Gal3, Not Nucleocytoplasmic Trafficking of Gal3 and Gal80
    Article Snippet: Plasmids pOE180 [nuclear export sequence (NES)- ] and pOE182 [mutant NES (mNES)- ] were constructed as follows. .. The P -2GFP reporter plasmid, pOE33, was constructed by PCR-based and other molecular methods in pRS416 (New England Biolabs).

    Isolation:

    Article Title: Rapid GAL Gene Switch of Saccharomyces cerevisiae Depends on Nuclear Gal3, Not Nucleocytoplasmic Trafficking of Gal3 and Gal80
    Article Snippet: To introduce NES and mNES (L326 , I328 ) into , the region of -encoding residues (AA 316–357) was isolated from pGEM-T (AA 316–357) and pGEM-T (AA 316–357) ( ) by PCR with primers OE167 (5′-CTCGTCGACGTCGAAAAGGGAAATTTACCTGA-3′) and OE168 (5′-CTCGTCGACGTCCTCTTCAAAATCGTCAACCT-3′). .. The P -2GFP reporter plasmid, pOE33, was constructed by PCR-based and other molecular methods in pRS416 (New England Biolabs).

    Labeling:

    Article Title: A patterned anisotropic nanofluidic sieving structure for continuous-flow separation of DNA and proteins
    Article Snippet: .. The PCR marker as well as λ DNA–Hind III digest (New England BioLabs, Beverly, MA) were labeled with the intercalating fluorescence dye YOYO-1 (Molecular Probes, Eugene, OR) in TBE 5× buffer. .. The dye to DNA base pair ratio was about 1:2 and the final DNA concentration was about 42.18 μg/ml (PCR) and 104 μg/ml (λ DNA–Hind III digest).

    Purification:

    Article Title: Collection of cell-free DNA for genomic analysis of solid tumors in a clinical laboratory setting
    Article Snippet: Cell-free DNA was purified from plasma using the Qiagen Circulating Nucleic acids kit (Qiagen, Hilden, Germany). .. Size analysis was performed using gel electrophoresis on 2% agarose gels with PCR markers as size standards (New England Biolabs, Ipswich, MA).

    Sequencing:

    Article Title: Uptake of denatured collagen into hepatic stellate cells: evidence for the involvement of urokinase plasminogen activator receptor-associated protein/Endo180
    Article Snippet: Primers were designed using the Primer 3 Output program ( ) to amplify Endo180 in rat liver stellate cells from the human Endo180 mRNA sequence. .. PCR marker was provided by New England Biolabs (Beverly, MA, U.S.A.).

    Article Title: The association between XRCC1 polymorphism and laryngeal cancer susceptibility in different ethnic groups in Xinjiang, China
    Article Snippet: A PCR Marker was purchased from NEW ENGLAND Biolabs. .. A 5X Sequencing Buffer was purchased from ABI, America.

    Article Title: Transposable Element Insertion and Epigenetic Modification Cause the Multiallelic Variation in the Expression of FAE1 in Sinapis alba
    Article Snippet: ) was designed based on the sequences of the promoter and the transposon Sal- PIF inserted in the coding region of FAE1 allele e ) was designed based on the promoter sequence and the sequence of the retrotransposon Sal- T1 in the 5′ of FAE1 alleles E2 and E3 . .. Each PCR (20 µL) contained 1× PCR standard buffer (NEB), 200 µM each deoxynucleotide triphosphate, 0.1 µM of each forward and reverse primer, 1 unit of Taq polymerase (NEB), and 50 ng of genomic DNA.

    Article Title: Rapid GAL Gene Switch of Saccharomyces cerevisiae Depends on Nuclear Gal3, Not Nucleocytoplasmic Trafficking of Gal3 and Gal80
    Article Snippet: Plasmids pOE180 [nuclear export sequence (NES)- ] and pOE182 [mutant NES (mNES)- ] were constructed as follows. .. The P -2GFP reporter plasmid, pOE33, was constructed by PCR-based and other molecular methods in pRS416 (New England Biolabs).

    Construct:

    Article Title: Rapid GAL Gene Switch of Saccharomyces cerevisiae Depends on Nuclear Gal3, Not Nucleocytoplasmic Trafficking of Gal3 and Gal80
    Article Snippet: .. The P -2GFP reporter plasmid, pOE33, was constructed by PCR-based and other molecular methods in pRS416 (New England Biolabs). ..

    Agarose Gel Electrophoresis:

    Article Title: Identification of genes for normalization of real-time RT-PCR data in breast carcinomas
    Article Snippet: .. The products were run on a 3% NuSieve GTG agarose gel (Cambrex Bioscience Rockland Inc., Rockland, ME) with 20 μg EtBr (Sigma, St. Louis, MO) in 1× TBE buffer (Sigma) at 5 V/5 min followed by 100 V/45 min (BioRad, Hercules, CA) along with a PCR marker (New England BioLabs Inc., Ipswich, MA). .. ▪ Immunohistochemistry (IHC) The NCL-ER-6F11 antibody (Novocastra Ltd., Newcastle, UK) was used for ER detection.

    Plasmid Preparation:

    Article Title: Uptake of denatured collagen into hepatic stellate cells: evidence for the involvement of urokinase plasminogen activator receptor-associated protein/Endo180
    Article Snippet: Routine PCR was performed using DyNAzyme (Finnzymes, Espoo, Finland) according to the manufacturer's instructions and performed at the following conditions: 94 °C for 5 min, 35 cycles each 30 s at 94 °C, 60 °C for 30 s and 72 °C for 30 s. The PCR products were cloned into pGEM-T Easy Vector (Promega) and sequenced (GATC-Biotech, Konstanz, Germany). .. PCR marker was provided by New England Biolabs (Beverly, MA, U.S.A.).

    Article Title: Rapid GAL Gene Switch of Saccharomyces cerevisiae Depends on Nuclear Gal3, Not Nucleocytoplasmic Trafficking of Gal3 and Gal80
    Article Snippet: .. The P -2GFP reporter plasmid, pOE33, was constructed by PCR-based and other molecular methods in pRS416 (New England Biolabs). ..

    Multiplex Assay:

    Article Title: Collection of cell-free DNA for genomic analysis of solid tumors in a clinical laboratory setting
    Article Snippet: Size analysis was performed using gel electrophoresis on 2% agarose gels with PCR markers as size standards (New England Biolabs, Ipswich, MA). .. The spike-in DNA used in this study was structural multiplex cfDNA reference standard, product HD786, from Horizon (Cambridge, UK).

    Selection:

    Article Title: Rapid GAL Gene Switch of Saccharomyces cerevisiae Depends on Nuclear Gal3, Not Nucleocytoplasmic Trafficking of Gal3 and Gal80
    Article Snippet: The NAT selection marker in plasmids that were used as a source of genomic insertion cassettes was isolated from Toda10 (a gift from Jian-Qiu Wu). pFJ04 was constructed by insertion of Sma I/ Xho I fragments of pGP15 ( ) and pSA80N1 (S. Alam and J. E. Hopper, unpublished results) into pRS416 (New England Biolabs). pFJ109N was constructed by simultaneous insertion of the NAT marker and a 2GFP cassette obtained from pKT209 (Sheff and Thron 2004) into pFJ04 by PCR-based and other molecular methods. pFJ110N was constructed by insertion of the NAT marker and the 2GFP cassette into the -containing plasmid, pLM34 (L. Mylin and J. E. Hopper, unpublished results). pFJ120N was constructed by insertion of the NAT cassette into pGP17 ( ). .. The P -2GFP reporter plasmid, pOE33, was constructed by PCR-based and other molecular methods in pRS416 (New England Biolabs).

    Sample Prep:

    Article Title: A patterned anisotropic nanofluidic sieving structure for continuous-flow separation of DNA and proteins
    Article Snippet: Paragraph title: Sample preparation ... The PCR marker as well as λ DNA–Hind III digest (New England BioLabs, Beverly, MA) were labeled with the intercalating fluorescence dye YOYO-1 (Molecular Probes, Eugene, OR) in TBE 5× buffer.

    Concentration Assay:

    Article Title: A patterned anisotropic nanofluidic sieving structure for continuous-flow separation of DNA and proteins
    Article Snippet: The PCR marker as well as λ DNA–Hind III digest (New England BioLabs, Beverly, MA) were labeled with the intercalating fluorescence dye YOYO-1 (Molecular Probes, Eugene, OR) in TBE 5× buffer. .. The dye to DNA base pair ratio was about 1:2 and the final DNA concentration was about 42.18 μg/ml (PCR) and 104 μg/ml (λ DNA–Hind III digest).

    Marker:

    Article Title: Uptake of denatured collagen into hepatic stellate cells: evidence for the involvement of urokinase plasminogen activator receptor-associated protein/Endo180
    Article Snippet: .. PCR marker was provided by New England Biolabs (Beverly, MA, U.S.A.). ..

    Article Title: Diepoxybutane Interstrand Cross-Links Induce DNA Bending
    Article Snippet: .. PCR marker (New England Biolabs) was used as a size reference (765 bp, 500 bp, 300 bp, 150 bp, and 50 bp, with the 300-bp marker resolving as two bands). ..

    Article Title: Identification of genes for normalization of real-time RT-PCR data in breast carcinomas
    Article Snippet: .. The products were run on a 3% NuSieve GTG agarose gel (Cambrex Bioscience Rockland Inc., Rockland, ME) with 20 μg EtBr (Sigma, St. Louis, MO) in 1× TBE buffer (Sigma) at 5 V/5 min followed by 100 V/45 min (BioRad, Hercules, CA) along with a PCR marker (New England BioLabs Inc., Ipswich, MA). .. ▪ Immunohistochemistry (IHC) The NCL-ER-6F11 antibody (Novocastra Ltd., Newcastle, UK) was used for ER detection.

    Article Title: The association between XRCC1 polymorphism and laryngeal cancer susceptibility in different ethnic groups in Xinjiang, China
    Article Snippet: .. A PCR Marker was purchased from NEW ENGLAND Biolabs. .. BIOWEST agarose was purchased from Shanghai Xiayi Industrial Co., LTD.

    Article Title: Transposable Element Insertion and Epigenetic Modification Cause the Multiallelic Variation in the Expression of FAE1 in Sinapis alba
    Article Snippet: The cosegregation of the marker specific for the allele e and zero erucic content was studied in 96 F2 plants derived from the cross Y514 ( ee ) × Y517 ( E1 E1 ), while the cosegregation of the marker specific for the allele E2 with medium erucic content was studied in 70 F2 plants of the cross Y496 ( E2 E2 ) × Y517 ( E1 E1 ). .. Each PCR (20 µL) contained 1× PCR standard buffer (NEB), 200 µM each deoxynucleotide triphosphate, 0.1 µM of each forward and reverse primer, 1 unit of Taq polymerase (NEB), and 50 ng of genomic DNA.

    Article Title: A patterned anisotropic nanofluidic sieving structure for continuous-flow separation of DNA and proteins
    Article Snippet: .. The PCR marker as well as λ DNA–Hind III digest (New England BioLabs, Beverly, MA) were labeled with the intercalating fluorescence dye YOYO-1 (Molecular Probes, Eugene, OR) in TBE 5× buffer. .. The dye to DNA base pair ratio was about 1:2 and the final DNA concentration was about 42.18 μg/ml (PCR) and 104 μg/ml (λ DNA–Hind III digest).

    Article Title: Rapid GAL Gene Switch of Saccharomyces cerevisiae Depends on Nuclear Gal3, Not Nucleocytoplasmic Trafficking of Gal3 and Gal80
    Article Snippet: The NAT selection marker in plasmids that were used as a source of genomic insertion cassettes was isolated from Toda10 (a gift from Jian-Qiu Wu). pFJ04 was constructed by insertion of Sma I/ Xho I fragments of pGP15 ( ) and pSA80N1 (S. Alam and J. E. Hopper, unpublished results) into pRS416 (New England Biolabs). pFJ109N was constructed by simultaneous insertion of the NAT marker and a 2GFP cassette obtained from pKT209 (Sheff and Thron 2004) into pFJ04 by PCR-based and other molecular methods. pFJ110N was constructed by insertion of the NAT marker and the 2GFP cassette into the -containing plasmid, pLM34 (L. Mylin and J. E. Hopper, unpublished results). pFJ120N was constructed by insertion of the NAT cassette into pGP17 ( ). .. The P -2GFP reporter plasmid, pOE33, was constructed by PCR-based and other molecular methods in pRS416 (New England Biolabs).

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  • 95
    New England Biolabs pcr marker
    O Ogston sieving of short <t>DNA</t> (the <t>PCR</t> marker) through the ANA
    Pcr Marker, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 12235 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr marker/product/New England Biolabs
    Average 95 stars, based on 12235 article reviews
    Price from $9.99 to $1999.99
    pcr marker - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

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    O Ogston sieving of short DNA (the PCR marker) through the ANA

    Journal:

    Article Title: A patterned anisotropic nanofluidic sieving structure for continuous-flow separation of DNA and proteins

    doi: 10.1038/nnano.2006.206

    Figure Lengend Snippet: O Ogston sieving of short DNA (the PCR marker) through the ANA

    Article Snippet: The PCR marker as well as λ DNA–Hind III digest (New England BioLabs, Beverly, MA) were labeled with the intercalating fluorescence dye YOYO-1 (Molecular Probes, Eugene, OR) in TBE 5× buffer.

    Techniques: Polymerase Chain Reaction, Marker