pcr marker  (New England Biolabs)


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    Name:
    PCR Marker
    Description:
    PCR Marker 500 gel lanes
    Catalog Number:
    n3234l
    Price:
    272
    Size:
    500 gel lanes
    Category:
    PCR Molecular Weight Markers
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    Structured Review

    New England Biolabs pcr marker
    PCR Marker
    PCR Marker 500 gel lanes
    https://www.bioz.com/result/pcr marker/product/New England Biolabs
    Average 97 stars, based on 16667 article reviews
    Price from $9.99 to $1999.99
    pcr marker - by Bioz Stars, 2020-07
    97/100 stars

    Images

    1) Product Images from "A patterned anisotropic nanofluidic sieving structure for continuous-flow separation of DNA and proteins"

    Article Title: A patterned anisotropic nanofluidic sieving structure for continuous-flow separation of DNA and proteins

    Journal:

    doi: 10.1038/nnano.2006.206

    O Ogston sieving of short DNA (the PCR marker) through the ANA
    Figure Legend Snippet: O Ogston sieving of short DNA (the PCR marker) through the ANA

    Techniques Used: Polymerase Chain Reaction, Marker

    Related Articles

    Amplification:

    Article Title: The Cysteine Rich Necrotrophic Effector SnTox1 Produced by Stagonospora nodorum Triggers Susceptibility of Wheat Lines Harboring Snn1
    Article Snippet: .. Standard PCR conditions and Taq polymerase (NEB BioLabs) were used for both rounds of amplification except that round 2 used a longer extension time due to the longer template. ..

    Article Title: The BpeAB-OprB Efflux Pump of Burkholderia pseudomallei 1026b Does Not Play a Role in Quorum Sensing, Virulence Factor Production, or Extrusion of Aminoglycosides but Is a Broad-Spectrum Drug Efflux System ▿
    Article Snippet: .. Briefly, using 5 to 50 ng of chromosomal or plasmid DNA templates, three partially overlapping DNA fragments, representing flanking DNA segments and a fragment encoding an antibiotic resistance marker, were PCR amplified separately using Taq polymerase (New England Biolabs, Ipswich, MA) and then spliced together by overlap extension PCR. .. For bpeR , the amplified fragments were an 883-bp bpeR upstream fragment, amplified with primers bpeR-UP-For and bpeR-UP-Rev-GM; an 875-bp bpeR downstream fragment, amplified with primers bpeR-DN-For-GM and bpeR-DN-Rev; and a 776-bp zeocin resistance-encoding ble fragment from pFZE1 , amplified using GmFRT-UP and GmFRT-DN.

    Article Title: Molecular Mapping of PMR1, a Novel Locus Conferring Resistance to Powdery Mildew in Pepper (Capsicum annuum)
    Article Snippet: .. The PCR program was 95°C for 5 min, 35 cycles of 95°C for 30 s, 56°C for 30 s, and 72°C for 60 s, followed by a final extension of 72°C for 10 min. For genotyping with CAPS marker (CZ2_11628), PCR was performed as mentioned above, but with an extension time of 72°C for 30 s. Amplified PCR products were digested with the restriction enzyme Taq α I according to the manufacturer's protocol (New England Biolabs, Beverly, MA). .. Digested PCR products were separated by gel electrophoresis and observed under a UV transilluminator (Bio-Rad, USA).

    Agarose Gel Electrophoresis:

    Article Title: A citizen science model for implementing statewide educational DNA barcoding
    Article Snippet: .. The PCR temperature cycle on the BioRad model T100 thermocyclers incorporated denaturation at 98°C for 30 s, annealing at 48°C for 30 s, and primer extension at 72°C for 45 s. The cycle was repeated 25–30 times and bracketed by an initial denaturation step at 98°C for 2 min and a concluding extension step at 72°C for 5 min. PCR products were evaluated by 1% agarose gel electrophoresis stained by SYBRSafe (Invitrogen, USA) and compared against size standards in Quick Load PCR Marker #N0475S (New England Biolabs, USA). .. After the student workshops were completed, PCR products were purified using the QIAquick PCR Purification Kit (Qiagen, Germany) following the manufacturer’s protocol.

    Fluorescence:

    Article Title: A patterned anisotropic nanofluidic sieving structure for continuous-flow separation of DNA and proteins
    Article Snippet: .. The PCR marker as well as λ DNA–Hind III digest (New England BioLabs, Beverly, MA) were labeled with the intercalating fluorescence dye YOYO-1 (Molecular Probes, Eugene, OR) in TBE 5× buffer. .. The dye to DNA base pair ratio was about 1:2 and the final DNA concentration was about 42.18 μg/ml (PCR) and 104 μg/ml (λ DNA–Hind III digest).

    Labeling:

    Article Title: A patterned anisotropic nanofluidic sieving structure for continuous-flow separation of DNA and proteins
    Article Snippet: .. The PCR marker as well as λ DNA–Hind III digest (New England BioLabs, Beverly, MA) were labeled with the intercalating fluorescence dye YOYO-1 (Molecular Probes, Eugene, OR) in TBE 5× buffer. .. The dye to DNA base pair ratio was about 1:2 and the final DNA concentration was about 42.18 μg/ml (PCR) and 104 μg/ml (λ DNA–Hind III digest).

    Polymerase Chain Reaction:

    Article Title: A citizen science model for implementing statewide educational DNA barcoding
    Article Snippet: .. The PCR temperature cycle on the BioRad model T100 thermocyclers incorporated denaturation at 98°C for 30 s, annealing at 48°C for 30 s, and primer extension at 72°C for 45 s. The cycle was repeated 25–30 times and bracketed by an initial denaturation step at 98°C for 2 min and a concluding extension step at 72°C for 5 min. PCR products were evaluated by 1% agarose gel electrophoresis stained by SYBRSafe (Invitrogen, USA) and compared against size standards in Quick Load PCR Marker #N0475S (New England Biolabs, USA). .. After the student workshops were completed, PCR products were purified using the QIAquick PCR Purification Kit (Qiagen, Germany) following the manufacturer’s protocol.

    Article Title: The Cysteine Rich Necrotrophic Effector SnTox1 Produced by Stagonospora nodorum Triggers Susceptibility of Wheat Lines Harboring Snn1
    Article Snippet: .. Standard PCR conditions and Taq polymerase (NEB BioLabs) were used for both rounds of amplification except that round 2 used a longer extension time due to the longer template. ..

    Article Title: A patterned anisotropic nanofluidic sieving structure for continuous-flow separation of DNA and proteins
    Article Snippet: .. The PCR marker as well as λ DNA–Hind III digest (New England BioLabs, Beverly, MA) were labeled with the intercalating fluorescence dye YOYO-1 (Molecular Probes, Eugene, OR) in TBE 5× buffer. .. The dye to DNA base pair ratio was about 1:2 and the final DNA concentration was about 42.18 μg/ml (PCR) and 104 μg/ml (λ DNA–Hind III digest).

    Article Title: The BpeAB-OprB Efflux Pump of Burkholderia pseudomallei 1026b Does Not Play a Role in Quorum Sensing, Virulence Factor Production, or Extrusion of Aminoglycosides but Is a Broad-Spectrum Drug Efflux System ▿
    Article Snippet: .. Briefly, using 5 to 50 ng of chromosomal or plasmid DNA templates, three partially overlapping DNA fragments, representing flanking DNA segments and a fragment encoding an antibiotic resistance marker, were PCR amplified separately using Taq polymerase (New England Biolabs, Ipswich, MA) and then spliced together by overlap extension PCR. .. For bpeR , the amplified fragments were an 883-bp bpeR upstream fragment, amplified with primers bpeR-UP-For and bpeR-UP-Rev-GM; an 875-bp bpeR downstream fragment, amplified with primers bpeR-DN-For-GM and bpeR-DN-Rev; and a 776-bp zeocin resistance-encoding ble fragment from pFZE1 , amplified using GmFRT-UP and GmFRT-DN.

    Article Title: Molecular Mapping of PMR1, a Novel Locus Conferring Resistance to Powdery Mildew in Pepper (Capsicum annuum)
    Article Snippet: .. The PCR program was 95°C for 5 min, 35 cycles of 95°C for 30 s, 56°C for 30 s, and 72°C for 60 s, followed by a final extension of 72°C for 10 min. For genotyping with CAPS marker (CZ2_11628), PCR was performed as mentioned above, but with an extension time of 72°C for 30 s. Amplified PCR products were digested with the restriction enzyme Taq α I according to the manufacturer's protocol (New England Biolabs, Beverly, MA). .. Digested PCR products were separated by gel electrophoresis and observed under a UV transilluminator (Bio-Rad, USA).

    Marker:

    Article Title: A citizen science model for implementing statewide educational DNA barcoding
    Article Snippet: .. The PCR temperature cycle on the BioRad model T100 thermocyclers incorporated denaturation at 98°C for 30 s, annealing at 48°C for 30 s, and primer extension at 72°C for 45 s. The cycle was repeated 25–30 times and bracketed by an initial denaturation step at 98°C for 2 min and a concluding extension step at 72°C for 5 min. PCR products were evaluated by 1% agarose gel electrophoresis stained by SYBRSafe (Invitrogen, USA) and compared against size standards in Quick Load PCR Marker #N0475S (New England Biolabs, USA). .. After the student workshops were completed, PCR products were purified using the QIAquick PCR Purification Kit (Qiagen, Germany) following the manufacturer’s protocol.

    Article Title: A patterned anisotropic nanofluidic sieving structure for continuous-flow separation of DNA and proteins
    Article Snippet: .. The PCR marker as well as λ DNA–Hind III digest (New England BioLabs, Beverly, MA) were labeled with the intercalating fluorescence dye YOYO-1 (Molecular Probes, Eugene, OR) in TBE 5× buffer. .. The dye to DNA base pair ratio was about 1:2 and the final DNA concentration was about 42.18 μg/ml (PCR) and 104 μg/ml (λ DNA–Hind III digest).

    Article Title: The BpeAB-OprB Efflux Pump of Burkholderia pseudomallei 1026b Does Not Play a Role in Quorum Sensing, Virulence Factor Production, or Extrusion of Aminoglycosides but Is a Broad-Spectrum Drug Efflux System ▿
    Article Snippet: .. Briefly, using 5 to 50 ng of chromosomal or plasmid DNA templates, three partially overlapping DNA fragments, representing flanking DNA segments and a fragment encoding an antibiotic resistance marker, were PCR amplified separately using Taq polymerase (New England Biolabs, Ipswich, MA) and then spliced together by overlap extension PCR. .. For bpeR , the amplified fragments were an 883-bp bpeR upstream fragment, amplified with primers bpeR-UP-For and bpeR-UP-Rev-GM; an 875-bp bpeR downstream fragment, amplified with primers bpeR-DN-For-GM and bpeR-DN-Rev; and a 776-bp zeocin resistance-encoding ble fragment from pFZE1 , amplified using GmFRT-UP and GmFRT-DN.

    Article Title: Molecular Mapping of PMR1, a Novel Locus Conferring Resistance to Powdery Mildew in Pepper (Capsicum annuum)
    Article Snippet: .. The PCR program was 95°C for 5 min, 35 cycles of 95°C for 30 s, 56°C for 30 s, and 72°C for 60 s, followed by a final extension of 72°C for 10 min. For genotyping with CAPS marker (CZ2_11628), PCR was performed as mentioned above, but with an extension time of 72°C for 30 s. Amplified PCR products were digested with the restriction enzyme Taq α I according to the manufacturer's protocol (New England Biolabs, Beverly, MA). .. Digested PCR products were separated by gel electrophoresis and observed under a UV transilluminator (Bio-Rad, USA).

    Staining:

    Article Title: A citizen science model for implementing statewide educational DNA barcoding
    Article Snippet: .. The PCR temperature cycle on the BioRad model T100 thermocyclers incorporated denaturation at 98°C for 30 s, annealing at 48°C for 30 s, and primer extension at 72°C for 45 s. The cycle was repeated 25–30 times and bracketed by an initial denaturation step at 98°C for 2 min and a concluding extension step at 72°C for 5 min. PCR products were evaluated by 1% agarose gel electrophoresis stained by SYBRSafe (Invitrogen, USA) and compared against size standards in Quick Load PCR Marker #N0475S (New England Biolabs, USA). .. After the student workshops were completed, PCR products were purified using the QIAquick PCR Purification Kit (Qiagen, Germany) following the manufacturer’s protocol.

    Plasmid Preparation:

    Article Title: The BpeAB-OprB Efflux Pump of Burkholderia pseudomallei 1026b Does Not Play a Role in Quorum Sensing, Virulence Factor Production, or Extrusion of Aminoglycosides but Is a Broad-Spectrum Drug Efflux System ▿
    Article Snippet: .. Briefly, using 5 to 50 ng of chromosomal or plasmid DNA templates, three partially overlapping DNA fragments, representing flanking DNA segments and a fragment encoding an antibiotic resistance marker, were PCR amplified separately using Taq polymerase (New England Biolabs, Ipswich, MA) and then spliced together by overlap extension PCR. .. For bpeR , the amplified fragments were an 883-bp bpeR upstream fragment, amplified with primers bpeR-UP-For and bpeR-UP-Rev-GM; an 875-bp bpeR downstream fragment, amplified with primers bpeR-DN-For-GM and bpeR-DN-Rev; and a 776-bp zeocin resistance-encoding ble fragment from pFZE1 , amplified using GmFRT-UP and GmFRT-DN.

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  • Contact
  • Bioz Stars
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  • 97
    New England Biolabs pcr marker
    O Ogston sieving of short <t>DNA</t> (the <t>PCR</t> marker) through the ANA
    Pcr Marker, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 16352 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr marker/product/New England Biolabs
    Average 97 stars, based on 16352 article reviews
    Price from $9.99 to $1999.99
    pcr marker - by Bioz Stars, 2020-07
    97/100 stars
      Buy from Supplier

    Image Search Results


    O Ogston sieving of short DNA (the PCR marker) through the ANA

    Journal:

    Article Title: A patterned anisotropic nanofluidic sieving structure for continuous-flow separation of DNA and proteins

    doi: 10.1038/nnano.2006.206

    Figure Lengend Snippet: O Ogston sieving of short DNA (the PCR marker) through the ANA

    Article Snippet: The PCR marker as well as λ DNA–Hind III digest (New England BioLabs, Beverly, MA) were labeled with the intercalating fluorescence dye YOYO-1 (Molecular Probes, Eugene, OR) in TBE 5× buffer.

    Techniques: Polymerase Chain Reaction, Marker