1 kb plus dna ladder  (New England Biolabs)


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    Name:
    1 kb Plus DNA Ladder
    Description:
    2 Log DNA Ladder 500 1000 gel lanes
    Catalog Number:
    n3200l
    Price:
    231
    Size:
    1000 gel lanes
    Category:
    DNA Ladders
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    Structured Review

    New England Biolabs 1 kb plus dna ladder
    1 kb Plus DNA Ladder
    2 Log DNA Ladder 500 1000 gel lanes
    https://www.bioz.com/result/1 kb plus dna ladder/product/New England Biolabs
    Average 98 stars, based on 17370 article reviews
    Price from $9.99 to $1999.99
    1 kb plus dna ladder - by Bioz Stars, 2020-09
    98/100 stars

    Images

    1) Product Images from "The role of Rif1 in telomere length regulation is separable from its role in origin firing"

    Article Title: The role of Rif1 in telomere length regulation is separable from its role in origin firing

    Journal: eLife

    doi: 10.7554/eLife.58066

    DDK overexpression increases origin activation across the genome. ( A ) The DNA content of the arrested cells in Figure 2A and B was measured using flow cytometry. ( B ) The DNA content of the arrested cells in Figure 2C was measured using flow cytometry. ( C ) Relative copy number for the entire Chromosome VI is shown. WT is in purple, ∆N-dbf4 o/e is in orange, and ∆N-dbf4 Cdc7 o/e is in blue, relating to Figure 2C . ( D ) The relative copy number for 1 kb around the midpoint of each origin was calculated for each strain. WT is in purple, ∆N-dbf4 o/e is in orange, and ∆ N-dbf4 Cdc7 o/e is in blue. For each peak, the WT value was subtracted from the value of the over-expression strain and multiplied by 100. Median and interquartile range are plotted over the distribution, and * indicates a significant difference by one-sided Wilcoxon signed rank test: N-dbf4 o/e (p
    Figure Legend Snippet: DDK overexpression increases origin activation across the genome. ( A ) The DNA content of the arrested cells in Figure 2A and B was measured using flow cytometry. ( B ) The DNA content of the arrested cells in Figure 2C was measured using flow cytometry. ( C ) Relative copy number for the entire Chromosome VI is shown. WT is in purple, ∆N-dbf4 o/e is in orange, and ∆N-dbf4 Cdc7 o/e is in blue, relating to Figure 2C . ( D ) The relative copy number for 1 kb around the midpoint of each origin was calculated for each strain. WT is in purple, ∆N-dbf4 o/e is in orange, and ∆ N-dbf4 Cdc7 o/e is in blue. For each peak, the WT value was subtracted from the value of the over-expression strain and multiplied by 100. Median and interquartile range are plotted over the distribution, and * indicates a significant difference by one-sided Wilcoxon signed rank test: N-dbf4 o/e (p

    Techniques Used: Over Expression, Activation Assay, Flow Cytometry

    Mutations in Rif1 that increase telomere length do not affect origin firing across the genome. ( A ) The DNA content of the arrested cells in Figure 4C was measured using flow cytometry. ( B ) The DNA content of the arrested cells in Figure 4D was measured using flow cytometry. ( C ) Relative copy number for the entire Chromosome VI is shown. WT is in purple, rif1-∆1322 is in orange, and rif1 HOOK is in blue, relating to Figure 4D . ( D ) The relative copy number for 1 kb around the midpoint of each origin was calculated for each strain. For each peak, the WT value was subtracted from RIF1 mutant value and multiplied by 100. Median and interquartile range are plotted over the distribution, and one-sided Wilcoxon signed rank test used to test significance: rif1-∆1322 (p=1) and rif1 HOOK (p=1). ( E ) Heatmap of relative copy number for 10 kb region centered at each OriDB origin. Orange indicates high copy number and blue indicates low copy number. Origins are sorted based on highest copy number to lowest copy number.
    Figure Legend Snippet: Mutations in Rif1 that increase telomere length do not affect origin firing across the genome. ( A ) The DNA content of the arrested cells in Figure 4C was measured using flow cytometry. ( B ) The DNA content of the arrested cells in Figure 4D was measured using flow cytometry. ( C ) Relative copy number for the entire Chromosome VI is shown. WT is in purple, rif1-∆1322 is in orange, and rif1 HOOK is in blue, relating to Figure 4D . ( D ) The relative copy number for 1 kb around the midpoint of each origin was calculated for each strain. For each peak, the WT value was subtracted from RIF1 mutant value and multiplied by 100. Median and interquartile range are plotted over the distribution, and one-sided Wilcoxon signed rank test used to test significance: rif1-∆1322 (p=1) and rif1 HOOK (p=1). ( E ) Heatmap of relative copy number for 10 kb region centered at each OriDB origin. Orange indicates high copy number and blue indicates low copy number. Origins are sorted based on highest copy number to lowest copy number.

    Techniques Used: Flow Cytometry, Mutagenesis, Low Copy Number

    2) Product Images from "Practical utilization of recombinant AAV vector reference standards: focus on vector genomes titration by free ITR qPCR"

    Article Title: Practical utilization of recombinant AAV vector reference standards: focus on vector genomes titration by free ITR qPCR

    Journal: Molecular Therapy. Methods & Clinical Development

    doi: 10.1038/mtm.2016.19

    Generation of free ends for the plasmid inverted terminal repeats (ITRs). ( a ) Schematic representation of the plasmid psub201 and the PvuII and HindIII restriction sites. ( b ) Magnification of the plasmid DNA sequences close to the PvuII digestion sites. pEMBL8(+) plasmid backbone (lower case); AAV2-sub201 viral genome (upper case) and PvuII site (CAG/CTG): underlined. ( c ) Separation of undigested and digested plasmid DNA on a 1% agarose gel; supercoiled and linear DNA ladder were used as electrophoresis standards. ( d ) Plasmid DNA purity and concentration measured by spectrophotometry.
    Figure Legend Snippet: Generation of free ends for the plasmid inverted terminal repeats (ITRs). ( a ) Schematic representation of the plasmid psub201 and the PvuII and HindIII restriction sites. ( b ) Magnification of the plasmid DNA sequences close to the PvuII digestion sites. pEMBL8(+) plasmid backbone (lower case); AAV2-sub201 viral genome (upper case) and PvuII site (CAG/CTG): underlined. ( c ) Separation of undigested and digested plasmid DNA on a 1% agarose gel; supercoiled and linear DNA ladder were used as electrophoresis standards. ( d ) Plasmid DNA purity and concentration measured by spectrophotometry.

    Techniques Used: Plasmid Preparation, CTG Assay, Agarose Gel Electrophoresis, Electrophoresis, Concentration Assay, Spectrophotometry

    Related Articles

    Marker:

    Article Title: Mucosa-Associated Bacterial Diversity in Relation to Human Terminal Ileum and Colonic Biopsy Samples ▿
    Article Snippet: .. 200 bp) against a DNA molecular weight marker (2-log DNA ladder N3200L; New England Biolabs Ltd., Herts, United Kingdom). ..

    Article Title: Longitudinal Analyses of Gut Mucosal Microbiotas in Ulcerative Colitis in Relation to Patient Age and Disease Severity and Duration
    Article Snippet: .. Plasmid concentrations were determined by electrophoresis and comparison of band strengths against molecular marker DNA (2-log DNA ladder N3200L; New England BioLabs Ltd., Herts, United Kingdom) and by using a spectrophotometer (Cecil Instruments, Cambridge, United Kingdom) at 260/280 nm. ..

    Article Title: Boosting isoprene production via heterologous expression of the Kudzu isoprene synthase gene (kIspS) into Bacillus spp. cell factory
    Article Snippet: .. Marker: 2 log DNA ladder (1.0–10.0 kb) NEB catalogue #3 N3200, Lane 1: pET28b digested by Nco I and Not I (5.2 kb), Lane 2: kIspS (1.7 kb) digested by Nco I 4 and Not I. .. Colony PCR results of the recombinant plasmid pET28b-kIspS -C term in BL21 (DE3) cells.

    Article Title: Boosting isoprene production via heterologous expression of the Kudzu isoprene synthase gene (kIspS) into Bacillus spp. cell factory
    Article Snippet: .. Marker: 2 log DNA ladder (1.0–10.0 kb) NEB catalogue #N04695, 12 Lane 1: pHT01 (7.9 kb) digested by Bam HI and Xba I, Lane 2: amplified kIspS fragment (1.7 kb) 13 digested by Bam HI and Xba I. .. PCR screening results for recombinant B. subtilis and B. licheniformis harboring the 15 pHT01-kIspS plasmid.

    Amplification:

    Article Title: Boosting isoprene production via heterologous expression of the Kudzu isoprene synthase gene (kIspS) into Bacillus spp. cell factory
    Article Snippet: .. Marker: 2 log DNA ladder (1.0–10.0 kb) NEB catalogue #N04695, 12 Lane 1: pHT01 (7.9 kb) digested by Bam HI and Xba I, Lane 2: amplified kIspS fragment (1.7 kb) 13 digested by Bam HI and Xba I. .. PCR screening results for recombinant B. subtilis and B. licheniformis harboring the 15 pHT01-kIspS plasmid.

    Agarose Gel Electrophoresis:

    Article Title: Practical utilization of recombinant AAV vector reference standards: focus on vector genomes titration by free ITR qPCR
    Article Snippet: .. One hundred ng of each supercoiled and linearized plasmids were analyzed on a 1% native agarose gel alongside a 2-log DNA ladder and supercoiled ladder (New England BioLabs Cat N3200S and N0472S, Ipswich, MA) to confirm complete digestion and purity, and to confirm concentration. .. The concentration determined for each of these templates using the nanophotometer was used to calculate the number of copies of DNA present in each series of dilutions.

    Article Title: ATF6 Is Mutated in Early Onset Photoreceptor Degeneration With Macular Involvement
    Article Snippet: .. PCR products were run on 2% agarose gel stained with ethidium bromide along with 1-kb-plus ladder (New England Biolabs, Ipswich, MA, USA). ..

    Spectrophotometry:

    Article Title: Longitudinal Analyses of Gut Mucosal Microbiotas in Ulcerative Colitis in Relation to Patient Age and Disease Severity and Duration
    Article Snippet: .. Plasmid concentrations were determined by electrophoresis and comparison of band strengths against molecular marker DNA (2-log DNA ladder N3200L; New England BioLabs Ltd., Herts, United Kingdom) and by using a spectrophotometer (Cecil Instruments, Cambridge, United Kingdom) at 260/280 nm. ..

    Electrophoresis:

    Article Title: Longitudinal Analyses of Gut Mucosal Microbiotas in Ulcerative Colitis in Relation to Patient Age and Disease Severity and Duration
    Article Snippet: .. Plasmid concentrations were determined by electrophoresis and comparison of band strengths against molecular marker DNA (2-log DNA ladder N3200L; New England BioLabs Ltd., Herts, United Kingdom) and by using a spectrophotometer (Cecil Instruments, Cambridge, United Kingdom) at 260/280 nm. ..

    Concentration Assay:

    Article Title: Practical utilization of recombinant AAV vector reference standards: focus on vector genomes titration by free ITR qPCR
    Article Snippet: .. One hundred ng of each supercoiled and linearized plasmids were analyzed on a 1% native agarose gel alongside a 2-log DNA ladder and supercoiled ladder (New England BioLabs Cat N3200S and N0472S, Ipswich, MA) to confirm complete digestion and purity, and to confirm concentration. .. The concentration determined for each of these templates using the nanophotometer was used to calculate the number of copies of DNA present in each series of dilutions.

    Plasmid Preparation:

    Article Title: Longitudinal Analyses of Gut Mucosal Microbiotas in Ulcerative Colitis in Relation to Patient Age and Disease Severity and Duration
    Article Snippet: .. Plasmid concentrations were determined by electrophoresis and comparison of band strengths against molecular marker DNA (2-log DNA ladder N3200L; New England BioLabs Ltd., Herts, United Kingdom) and by using a spectrophotometer (Cecil Instruments, Cambridge, United Kingdom) at 260/280 nm. ..

    Polymerase Chain Reaction:

    Article Title: ATF6 Is Mutated in Early Onset Photoreceptor Degeneration With Macular Involvement
    Article Snippet: .. PCR products were run on 2% agarose gel stained with ethidium bromide along with 1-kb-plus ladder (New England Biolabs, Ipswich, MA, USA). ..

    Staining:

    Article Title: ATF6 Is Mutated in Early Onset Photoreceptor Degeneration With Macular Involvement
    Article Snippet: .. PCR products were run on 2% agarose gel stained with ethidium bromide along with 1-kb-plus ladder (New England Biolabs, Ipswich, MA, USA). ..

    Molecular Weight:

    Article Title: Mucosa-Associated Bacterial Diversity in Relation to Human Terminal Ileum and Colonic Biopsy Samples ▿
    Article Snippet: .. 200 bp) against a DNA molecular weight marker (2-log DNA ladder N3200L; New England Biolabs Ltd., Herts, United Kingdom). ..

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  • 98
    New England Biolabs 1 kb plus dna ladder
    DDK overexpression increases origin activation across the genome. ( A ) The <t>DNA</t> content of the arrested cells in Figure 2A and B was measured using flow cytometry. ( B ) The DNA content of the arrested cells in Figure 2C was measured using flow cytometry. ( C ) Relative copy number for the entire Chromosome VI is shown. WT is in purple, ∆N-dbf4 o/e is in orange, and ∆N-dbf4 Cdc7 o/e is in blue, relating to Figure 2C . ( D ) The relative copy number for <t>1</t> kb around the midpoint of each origin was calculated for each strain. WT is in purple, ∆N-dbf4 o/e is in orange, and ∆ N-dbf4 Cdc7 o/e is in blue. For each peak, the WT value was subtracted from the value of the over-expression strain and multiplied by 100. Median and interquartile range are plotted over the distribution, and * indicates a significant difference by one-sided Wilcoxon signed rank test: N-dbf4 o/e (p
    1 Kb Plus Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1 kb plus dna ladder/product/New England Biolabs
    Average 98 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    1 kb plus dna ladder - by Bioz Stars, 2020-09
    98/100 stars
      Buy from Supplier

    Image Search Results


    DDK overexpression increases origin activation across the genome. ( A ) The DNA content of the arrested cells in Figure 2A and B was measured using flow cytometry. ( B ) The DNA content of the arrested cells in Figure 2C was measured using flow cytometry. ( C ) Relative copy number for the entire Chromosome VI is shown. WT is in purple, ∆N-dbf4 o/e is in orange, and ∆N-dbf4 Cdc7 o/e is in blue, relating to Figure 2C . ( D ) The relative copy number for 1 kb around the midpoint of each origin was calculated for each strain. WT is in purple, ∆N-dbf4 o/e is in orange, and ∆ N-dbf4 Cdc7 o/e is in blue. For each peak, the WT value was subtracted from the value of the over-expression strain and multiplied by 100. Median and interquartile range are plotted over the distribution, and * indicates a significant difference by one-sided Wilcoxon signed rank test: N-dbf4 o/e (p

    Journal: eLife

    Article Title: The role of Rif1 in telomere length regulation is separable from its role in origin firing

    doi: 10.7554/eLife.58066

    Figure Lengend Snippet: DDK overexpression increases origin activation across the genome. ( A ) The DNA content of the arrested cells in Figure 2A and B was measured using flow cytometry. ( B ) The DNA content of the arrested cells in Figure 2C was measured using flow cytometry. ( C ) Relative copy number for the entire Chromosome VI is shown. WT is in purple, ∆N-dbf4 o/e is in orange, and ∆N-dbf4 Cdc7 o/e is in blue, relating to Figure 2C . ( D ) The relative copy number for 1 kb around the midpoint of each origin was calculated for each strain. WT is in purple, ∆N-dbf4 o/e is in orange, and ∆ N-dbf4 Cdc7 o/e is in blue. For each peak, the WT value was subtracted from the value of the over-expression strain and multiplied by 100. Median and interquartile range are plotted over the distribution, and * indicates a significant difference by one-sided Wilcoxon signed rank test: N-dbf4 o/e (p

    Article Snippet: We loaded genomic digests and 100 ng of 2-log ladder (NEB N3200) onto a 1% agarose gel and subjected it to electrophoresis in 1XTTE overnight at 49V.

    Techniques: Over Expression, Activation Assay, Flow Cytometry

    Mutations in Rif1 that increase telomere length do not affect origin firing across the genome. ( A ) The DNA content of the arrested cells in Figure 4C was measured using flow cytometry. ( B ) The DNA content of the arrested cells in Figure 4D was measured using flow cytometry. ( C ) Relative copy number for the entire Chromosome VI is shown. WT is in purple, rif1-∆1322 is in orange, and rif1 HOOK is in blue, relating to Figure 4D . ( D ) The relative copy number for 1 kb around the midpoint of each origin was calculated for each strain. For each peak, the WT value was subtracted from RIF1 mutant value and multiplied by 100. Median and interquartile range are plotted over the distribution, and one-sided Wilcoxon signed rank test used to test significance: rif1-∆1322 (p=1) and rif1 HOOK (p=1). ( E ) Heatmap of relative copy number for 10 kb region centered at each OriDB origin. Orange indicates high copy number and blue indicates low copy number. Origins are sorted based on highest copy number to lowest copy number.

    Journal: eLife

    Article Title: The role of Rif1 in telomere length regulation is separable from its role in origin firing

    doi: 10.7554/eLife.58066

    Figure Lengend Snippet: Mutations in Rif1 that increase telomere length do not affect origin firing across the genome. ( A ) The DNA content of the arrested cells in Figure 4C was measured using flow cytometry. ( B ) The DNA content of the arrested cells in Figure 4D was measured using flow cytometry. ( C ) Relative copy number for the entire Chromosome VI is shown. WT is in purple, rif1-∆1322 is in orange, and rif1 HOOK is in blue, relating to Figure 4D . ( D ) The relative copy number for 1 kb around the midpoint of each origin was calculated for each strain. For each peak, the WT value was subtracted from RIF1 mutant value and multiplied by 100. Median and interquartile range are plotted over the distribution, and one-sided Wilcoxon signed rank test used to test significance: rif1-∆1322 (p=1) and rif1 HOOK (p=1). ( E ) Heatmap of relative copy number for 10 kb region centered at each OriDB origin. Orange indicates high copy number and blue indicates low copy number. Origins are sorted based on highest copy number to lowest copy number.

    Article Snippet: We loaded genomic digests and 100 ng of 2-log ladder (NEB N3200) onto a 1% agarose gel and subjected it to electrophoresis in 1XTTE overnight at 49V.

    Techniques: Flow Cytometry, Mutagenesis, Low Copy Number

    Generation of free ends for the plasmid inverted terminal repeats (ITRs). ( a ) Schematic representation of the plasmid psub201 and the PvuII and HindIII restriction sites. ( b ) Magnification of the plasmid DNA sequences close to the PvuII digestion sites. pEMBL8(+) plasmid backbone (lower case); AAV2-sub201 viral genome (upper case) and PvuII site (CAG/CTG): underlined. ( c ) Separation of undigested and digested plasmid DNA on a 1% agarose gel; supercoiled and linear DNA ladder were used as electrophoresis standards. ( d ) Plasmid DNA purity and concentration measured by spectrophotometry.

    Journal: Molecular Therapy. Methods & Clinical Development

    Article Title: Practical utilization of recombinant AAV vector reference standards: focus on vector genomes titration by free ITR qPCR

    doi: 10.1038/mtm.2016.19

    Figure Lengend Snippet: Generation of free ends for the plasmid inverted terminal repeats (ITRs). ( a ) Schematic representation of the plasmid psub201 and the PvuII and HindIII restriction sites. ( b ) Magnification of the plasmid DNA sequences close to the PvuII digestion sites. pEMBL8(+) plasmid backbone (lower case); AAV2-sub201 viral genome (upper case) and PvuII site (CAG/CTG): underlined. ( c ) Separation of undigested and digested plasmid DNA on a 1% agarose gel; supercoiled and linear DNA ladder were used as electrophoresis standards. ( d ) Plasmid DNA purity and concentration measured by spectrophotometry.

    Article Snippet: One hundred ng of each supercoiled and linearized plasmids were analyzed on a 1% native agarose gel alongside a 2-log DNA ladder and supercoiled ladder (New England BioLabs Cat N3200S and N0472S, Ipswich, MA) to confirm complete digestion and purity, and to confirm concentration.

    Techniques: Plasmid Preparation, CTG Assay, Agarose Gel Electrophoresis, Electrophoresis, Concentration Assay, Spectrophotometry