pbr322 dna msp (New England Biolabs)
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Name:
pBR322 DNA Msp I Digest
Description:
pBR322 DNA Msp I Digest 250 gel lanes
Catalog Number:
n3032l
Price:
302
Size:
250 gel lanes
Category:
DNA Molecular Weight Markers
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New England Biolabs
pbr322 dna msp
pBR322 DNA Msp I Digest 250 gel lanes
https://www.bioz.com/result/pbr322 dna msp/product/New England Biolabs
Average 94 stars, based on 7 article reviews
Price from $9.99 to $1999.99
pBR322 DNA Msp I Digest 250 gel lanes
https://www.bioz.com/result/pbr322 dna msp/product/New England Biolabs
Average 94 stars, based on 7 article reviews
Price from $9.99 to $1999.99
pbr322 dna msp - by Bioz Stars,
2020-02
94/100 stars
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1) Product Images from "Fast IPSCs in rat thalamic reticular nucleus require the GABAA receptor ?1 subunit"
Article Title: Fast IPSCs in rat thalamic reticular nucleus require the GABAA receptor ?1 subunit
Journal: The Journal of Physiology
doi: 10.1113/jphysiol.2006.106617
Figure Legend Snippet: Cells with fast initial decay kinetics express β 1 subunit mRNA A and B , scatter plots of IPSC 90%-width versus peak current amplitude for two RTN cells recorded on the same day. Each point in A and B represents an individual IPSC. A is a recording from a cell dominated with slow and small IPSCs (○) and B , represents a recording dominated by brief and large IPSCs ( ). C , ensemble averaged IPSCs averaged from isolated spontaneous inhibitory events (selected on the basis of having arisen from an event-free baseline and showing complete decay to the same baseline) for the two cells represented in A (black, n = 258) and B (grey, n = 217). Each trace was best fitted to two exponentials (dotted line superimposed on each trace). Note: the cell dominated with fast IPSCs yielded a ensemble averaged IPSC with a larger A 1 value than A 2 whereas the opposite is true for the slower cell. D , ethidium bromide stained 8% polyacrylamide gel showing single-cell RT-PCR analysis from five RTN cells examined in one day of recording, two of the cells are represented in A (lane 3) and B (lane 7). Cells positive for the β 1 subunit cDNA showed a solitary band at 162 bp after two rounds of amplification using nested primers (lanes 6 and 7, see methods). Lanes 1 and 2 are water and media controls that underwent reverse transcription along with individual cells in lanes 3 through 7; lane 8 is a positive control using 40 pg of total RNA; lanes 9 and 10 are water controls used in the master mix for each round of PCR amplification. The molecular weight marker in lane 11 is the Msp I digest of pBR322. The size of each fragment is noted on the right. The parameters for β 1 negative cells in lane 4: A 1 = 9.80, τ 1 = 13.75, A 2 = 14.17, τ 2 = 64.61, τ DW = 43.82l; and lane 5: A 1 = 12.94, τ 1 = 12.94, A 2 = 5.69, τ 2 = 77.21, τ DW = 29.63. The β 1 positive cell in lane 6: A 1 = 18.05, τ 1 = 15.11, A 2 = 4.15, τ 2 = 74.44, τ DW = 26.20.
Techniques Used: Isolation, Staining, Reverse Transcription Polymerase Chain Reaction, Amplification, Positive Control, Polymerase Chain Reaction, Molecular Weight, Marker
Related Articles
Clone Assay:Article Title: Characterization of the Genes Encoding d-Amino Acid Transaminase and Glutamate Racemase, Two d-Glutamate Biosynthetic Enzymes of Bacillus sphaericus ATCC 10208 Article Snippet: Paragraph title: Cloning of d -glutamate biosynthetic genes from B. sphaericus. ... Where appropriate, chloramphenicol (10 μg/ml) and ampicillin (200 μg/ml) were added. Amplification:Article Title: Nontuberculous Mycobacterial Lung Disease Caused by Mycobacterium chelonae: A Case Report Article Snippet: Amplification of the partial rpoB gene (360 bp) was performed using primers Rpo5' (5'-TCAAGGAGAAGCGCTACGA-3') and Rpo3' (5'-GGATGTTGATCAGGGTCTGC-3') with subsequent digestion with 5 units of Msp I (New England BioLabs, Beverly, MA, USA) for 3 hours at 37℃. .. DNA size markers, Article Title: NeuroD1 Gene and Interleukin-18 Gene Polymorphisms in Type 1 Diabetes in the Dalmatian Population of Southern Croatia Article Snippet: For determination of fragment length DNA marker, Article Title: Fast IPSCs in rat thalamic reticular nucleus require the GABAA receptor ?1 subunit Article Snippet: Amplification products were analysed on either a 2% agarose gel or an 8% polyacrylamide gel and always yielded a single ethidium bromide stained-band that migrated at the predicted molecular weight of 162 bp. .. The molecular weight marker was the Expressing:Article Title: The Epstein-Barr virus lytic program is controlled by the co-operative functions of two transactivators Article Snippet: The labeled oligonucleotide was purified on a 10% acrylamide gel and annealed with mRNAs from the B95.8 cell line, from 293 cells transfected with an expression plasmid coding for BRLF1 or from 293-BRLF1-KO cells transfected with an expression plasmid coding for BRLF1. .. Positive Control:Article Title: Goniothalamin-induced oxidative stress, DNA damage and apoptosis via caspase-2 independent and Bcl-2 independent pathways in Jurkat T-cells Article Snippet: Topoisomerase II enzyme, GTN, and etoposide as a positive control, at the indicated concentrations were incubated in the buffer on ice for 10 min. .. Reactions contained 6 units (∼13 nM) of human topoisomerase IIα (TopoGen) and 300 ng of Nuclear Magnetic Resonance:Article Title: Mechanistic Studies of the Anticancer Activity of An Octahedral Hexanuclear Pt(II) Cage Article Snippet: Calf-thymus DNA was purchased from Invitrogen, Activity Assay:Article Title: Goniothalamin-induced oxidative stress, DNA damage and apoptosis via caspase-2 independent and Bcl-2 independent pathways in Jurkat T-cells Article Snippet: Reactions contained 6 units (∼13 nM) of human topoisomerase IIα (TopoGen) and 300 ng of Mass Spectrometry:Article Title: Mechanistic Studies of the Anticancer Activity of An Octahedral Hexanuclear Pt(II) Cage Article Snippet: Calf-thymus DNA was purchased from Invitrogen, Acrylamide Gel Assay:Article Title: The Epstein-Barr virus lytic program is controlled by the co-operative functions of two transactivators Article Snippet: After reverse transcription with Superscript™II Reverse Transcriptase (Life Technologies, Eggenstein, Germany) and addition of RNase A, the samples were separated on an 8% denaturing acrylamide gel. .. Derivative Assay:Article Title: Characterization of the Genes Encoding d-Amino Acid Transaminase and Glutamate Racemase, Two d-Glutamate Biosynthetic Enzymes of Bacillus sphaericus ATCC 10208 Article Snippet: Where appropriate, chloramphenicol (10 μg/ml) and ampicillin (200 μg/ml) were added. Transfection:Article Title: The Epstein-Barr virus lytic program is controlled by the co-operative functions of two transactivators Article Snippet: The labeled oligonucleotide was purified on a 10% acrylamide gel and annealed with mRNAs from the B95.8 cell line, from 293 cells transfected with an expression plasmid coding for BRLF1 or from 293-BRLF1-KO cells transfected with an expression plasmid coding for BRLF1. .. 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The molecular weight marker was the other:Article Title: Cylindrical Illumination Confocal Spectroscopy: Rectifying the Limitations of Confocal Single Molecule Spectroscopy through One-Dimensional Beam Shaping Article Snippet: Illumination powers of 1.85 mW/cm2 and 0.057 mW/cm2 were used for CICS and SMD analysis of pBR322 DNA, respectively. Article Title: Cylindrical Illumination Confocal Spectroscopy: Rectifying the Limitations of Confocal Single Molecule Spectroscopy through One-Dimensional Beam Shaping Article Snippet: For 488-CICS analysis of pBR322 DNA, the cylindrical lens is inserted into the beam path, and the circular pinhole is swapped for a 620 × 115 μ m rectangular confocal aperture. Negative Control:Article Title: Fast IPSCs in rat thalamic reticular nucleus require the GABAA receptor ?1 subunit Article Snippet: The molecular weight marker was the Polymerase Chain Reaction:Article Title: NeuroD1 Gene and Interleukin-18 Gene Polymorphisms in Type 1 Diabetes in the Dalmatian Population of Southern Croatia Article Snippet: For determination of fragment length DNA marker, Article Title: Fetal Mouse Cyp1b1 and Transplacental Carcinogenesis from Maternal Exposure to Dibenzo[a,l]pyrene Article Snippet: Cyp1b1 heterozygotes yielded two PCR products of 365-bp and 460-bp (NEO), respectively. .. A molecular weight ladder of Msp I cut Molecular Weight:Article Title: The Epstein-Barr virus lytic program is controlled by the co-operative functions of two transactivators Article Snippet: .. Article Title: Fast IPSCs in rat thalamic reticular nucleus require the GABAA receptor ?1 subunit Article Snippet: .. The molecular weight marker was the Article Title: Fetal Mouse Cyp1b1 and Transplacental Carcinogenesis from Maternal Exposure to Dibenzo[a,l]pyrene Article Snippet: .. A molecular weight ladder of Msp I cut Nucleic Acid Electrophoresis:Article Title: Nucleoprotein complex intermediates in HIV-1 integration Article Snippet: 2 Mix and incubate on ice for 30 min. 3 Add: 0.5 μl 500 ng/μl Mutagenesis:Article Title: Locus-specific control of the de novo DNA methylation pathway in Arabidopsis by the CLASSY family Article Snippet: Small RNA isolation: 4 un-opened flower buds (stage 12 and younger) from individual mutant plants as well as 3 individual wild-type (WT) controls were collected, frozen in liquid nitrogen and kept at −80°C until use. .. The final library products were further purified using an 8% polyacrylamide gel to excise 130–160nt products relative to the Article Title: Mammalian NET-seq analysis defines nascent RNA profiles and associated RNA processing genome-wide Article Snippet: MgCl2 , 1 M (Sigma-Aldrich, cat. no. M1028) NaCl (VWR Chemicals, cat. no. 27810.295) NP-40, IGEPAL CA630 (Sigma, cat. no. I8896) Nuclease-free water, not DEPC-Treated (Life technologies, cat. no. AM9937) Isolation:Article Title: Nontuberculous Mycobacterial Lung Disease Caused by Mycobacterium chelonae: A Case Report Article Snippet: NTM were isolated three times from sputum specimens. .. DNA size markers, Article Title: Locus-specific control of the de novo DNA methylation pathway in Arabidopsis by the CLASSY family Article Snippet: Paragraph title: Small RNA isolation: ... The final library products were further purified using an 8% polyacrylamide gel to excise 130–160nt products relative to the Silver Staining:Article Title: NeuroD1 Gene and Interleukin-18 Gene Polymorphisms in Type 1 Diabetes in the Dalmatian Population of Southern Croatia Article Snippet: The digested fragments were separated on 10% polyacrylamide gels and visualized by silver staining ( ). .. For determination of fragment length DNA marker, RNA Extraction:Article Title: Locus-specific control of the de novo DNA methylation pathway in Arabidopsis by the CLASSY family Article Snippet: The total RNA extraction and small RNA enrichment were performed as previously described with the following minor modifications: (1) for the small RNA enrichment step an equal volume of 20% polyethylene glycol 8000/2M NaCl was added to each total RNA sample and (2) the ZR-small RNA ladder (Zymo Research, Cat# R1090) was used to determine the gel region corresponding to the 17–29 nucleotide (nt) size range. .. The final library products were further purified using an 8% polyacrylamide gel to excise 130–160nt products relative to the Labeling:Article Title: The Epstein-Barr virus lytic program is controlled by the co-operative functions of two transactivators Article Snippet: The labeled oligonucleotide was purified on a 10% acrylamide gel and annealed with mRNAs from the B95.8 cell line, from 293 cells transfected with an expression plasmid coding for BRLF1 or from 293-BRLF1-KO cells transfected with an expression plasmid coding for BRLF1. .. Mouse Assay:Article Title: Fetal Mouse Cyp1b1 and Transplacental Carcinogenesis from Maternal Exposure to Dibenzo[a,l]pyrene Article Snippet: Paragraph title: Genotyping of mice for the Cyp1b1 allele ... A molecular weight ladder of Msp I cut Reverse Transcription Polymerase Chain Reaction:Article Title: Fast IPSCs in rat thalamic reticular nucleus require the GABAA receptor ?1 subunit Article Snippet: Paragraph title: Single-cell RT-PCR ... The molecular weight marker was the Purification:Article Title: Mechanistic Studies of the Anticancer Activity of An Octahedral Hexanuclear Pt(II) Cage Article Snippet: The compounds Pt(bpy)Cl2 and 2 were prepared according to previous reports., All reagents were purchased from Strem, Aldrich, or Alfa and used without further purification under normal atmospheric conditions. .. Calf-thymus DNA was purchased from Invitrogen, Article Title: The Epstein-Barr virus lytic program is controlled by the co-operative functions of two transactivators Article Snippet: The labeled oligonucleotide was purified on a 10% acrylamide gel and annealed with mRNAs from the B95.8 cell line, from 293 cells transfected with an expression plasmid coding for BRLF1 or from 293-BRLF1-KO cells transfected with an expression plasmid coding for BRLF1. .. Article Title: Locus-specific control of the de novo DNA methylation pathway in Arabidopsis by the CLASSY family Article Snippet: .. The final library products were further purified using an 8% polyacrylamide gel to excise 130–160nt products relative to the Plasmid Preparation:Article Title: Goniothalamin-induced oxidative stress, DNA damage and apoptosis via caspase-2 independent and Bcl-2 independent pathways in Jurkat T-cells Article Snippet: Reactions were initiated by the addition of pBR322 plasmid DNA and transferred to a 37 °C water bath. .. Reactions contained 6 units (∼13 nM) of human topoisomerase IIα (TopoGen) and 300 ng of Article Title: Characterization of the Genes Encoding d-Amino Acid Transaminase and Glutamate Racemase, Two d-Glutamate Biosynthetic Enzymes of Bacillus sphaericus ATCC 10208 Article Snippet: Where appropriate, chloramphenicol (10 μg/ml) and ampicillin (200 μg/ml) were added. Article Title: The Epstein-Barr virus lytic program is controlled by the co-operative functions of two transactivators Article Snippet: The labeled oligonucleotide was purified on a 10% acrylamide gel and annealed with mRNAs from the B95.8 cell line, from 293 cells transfected with an expression plasmid coding for BRLF1 or from 293-BRLF1-KO cells transfected with an expression plasmid coding for BRLF1. .. Multiplex Assay:Article Title: Locus-specific control of the de novo DNA methylation pathway in Arabidopsis by the CLASSY family Article Snippet: The resulting small RNAs were then used for library preparation with the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, Cat# E7300) following the user’s manual. .. The final library products were further purified using an 8% polyacrylamide gel to excise 130–160nt products relative to the Agarose Gel Electrophoresis:Article Title: Characterization of the Genes Encoding d-Amino Acid Transaminase and Glutamate Racemase, Two d-Glutamate Biosynthetic Enzymes of Bacillus sphaericus ATCC 10208 Article Snippet: Where appropriate, chloramphenicol (10 μg/ml) and ampicillin (200 μg/ml) were added. Article Title: Fast IPSCs in rat thalamic reticular nucleus require the GABAA receptor ?1 subunit Article Snippet: Amplification products were analysed on either a 2% agarose gel or an 8% polyacrylamide gel and always yielded a single ethidium bromide stained-band that migrated at the predicted molecular weight of 162 bp. .. The molecular weight marker was the Size-exclusion Chromatography:Article Title: Fetal Mouse Cyp1b1 and Transplacental Carcinogenesis from Maternal Exposure to Dibenzo[a,l]pyrene Article Snippet: PCR cycling conditions were an initial 5 min 95°C enzyme activation step, followed by 35 cycles of: 30 sec at 95°C to denature the DNA; 30 sec at 55°C for primer annealing; 45 sec at 72°C for extension. .. A molecular weight ladder of Msp I cut Incubation:Article Title: Goniothalamin-induced oxidative stress, DNA damage and apoptosis via caspase-2 independent and Bcl-2 independent pathways in Jurkat T-cells Article Snippet: Topoisomerase II enzyme, GTN, and etoposide as a positive control, at the indicated concentrations were incubated in the buffer on ice for 10 min. .. Reactions contained 6 units (∼13 nM) of human topoisomerase IIα (TopoGen) and 300 ng of Produced:Article Title: NeuroD1 Gene and Interleukin-18 Gene Polymorphisms in Type 1 Diabetes in the Dalmatian Population of Southern Croatia Article Snippet: Homozygotes AA (Thr/Thr) produced two fragments, while the homozygote GG (Ala/Ala) produced one 198-bp fragment. .. For determination of fragment length DNA marker, Activation Assay:Article Title: Fetal Mouse Cyp1b1 and Transplacental Carcinogenesis from Maternal Exposure to Dibenzo[a,l]pyrene Article Snippet: PCR cycling conditions were an initial 5 min 95°C enzyme activation step, followed by 35 cycles of: 30 sec at 95°C to denature the DNA; 30 sec at 55°C for primer annealing; 45 sec at 72°C for extension. .. A molecular weight ladder of Msp I cut Marker:Article Title: NeuroD1 Gene and Interleukin-18 Gene Polymorphisms in Type 1 Diabetes in the Dalmatian Population of Southern Croatia Article Snippet: .. For determination of fragment length DNA marker, Article Title: Fast IPSCs in rat thalamic reticular nucleus require the GABAA receptor ?1 subunit Article Snippet: .. The molecular weight marker was the Staining:Article Title: Cylindrical Illumination Confocal Spectroscopy: Rectifying the Limitations of Confocal Single Molecule Spectroscopy through One-Dimensional Beam Shaping Article Snippet: .. For 488-SMD and 488-CICS analysis, Article Title: Fast IPSCs in rat thalamic reticular nucleus require the GABAA receptor ?1 subunit Article Snippet: Amplification products were analysed on either a 2% agarose gel or an 8% polyacrylamide gel and always yielded a single ethidium bromide stained-band that migrated at the predicted molecular weight of 162 bp. .. The molecular weight marker was the Article Title: Fetal Mouse Cyp1b1 and Transplacental Carcinogenesis from Maternal Exposure to Dibenzo[a,l]pyrene Article Snippet: .. A molecular weight ladder of Msp I cut |