pbr322 dna msp  (New England Biolabs)


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    Name:
    pBR322 DNA Msp I Digest
    Description:
    pBR322 DNA Msp I Digest 250 gel lanes
    Catalog Number:
    n3032l
    Price:
    302
    Size:
    250 gel lanes
    Category:
    DNA Molecular Weight Markers
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    New England Biolabs pbr322 dna msp
    pBR322 DNA Msp I Digest
    pBR322 DNA Msp I Digest 250 gel lanes
    https://www.bioz.com/result/pbr322 dna msp/product/New England Biolabs
    Average 94 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    pbr322 dna msp - by Bioz Stars, 2020-02
    94/100 stars

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    1) Product Images from "Fast IPSCs in rat thalamic reticular nucleus require the GABAA receptor ?1 subunit"

    Article Title: Fast IPSCs in rat thalamic reticular nucleus require the GABAA receptor ?1 subunit

    Journal: The Journal of Physiology

    doi: 10.1113/jphysiol.2006.106617

    Cells with fast initial decay kinetics express β 1 subunit mRNA A and B , scatter plots of IPSC 90%-width versus peak current amplitude for two RTN cells recorded on the same day. Each point in A and B represents an individual IPSC. A is a recording from a cell dominated with slow and small IPSCs (○) and B , represents a recording dominated by brief and large IPSCs ( ). C , ensemble averaged IPSCs averaged from isolated spontaneous inhibitory events (selected on the basis of having arisen from an event-free baseline and showing complete decay to the same baseline) for the two cells represented in A (black, n = 258) and B (grey, n = 217). Each trace was best fitted to two exponentials (dotted line superimposed on each trace). Note: the cell dominated with fast IPSCs yielded a ensemble averaged IPSC with a larger A 1 value than A 2 whereas the opposite is true for the slower cell. D , ethidium bromide stained 8% polyacrylamide gel showing single-cell RT-PCR analysis from five RTN cells examined in one day of recording, two of the cells are represented in A (lane 3) and B (lane 7). Cells positive for the β 1 subunit cDNA showed a solitary band at 162 bp after two rounds of amplification using nested primers (lanes 6 and 7, see methods). Lanes 1 and 2 are water and media controls that underwent reverse transcription along with individual cells in lanes 3 through 7; lane 8 is a positive control using 40 pg of total RNA; lanes 9 and 10 are water controls used in the master mix for each round of PCR amplification. The molecular weight marker in lane 11 is the Msp I digest of pBR322. The size of each fragment is noted on the right. The parameters for β 1 negative cells in lane 4: A 1 = 9.80, τ 1 = 13.75, A 2 = 14.17, τ 2 = 64.61, τ DW = 43.82l; and lane 5: A 1 = 12.94, τ 1 = 12.94, A 2 = 5.69, τ 2 = 77.21, τ DW = 29.63. The β 1 positive cell in lane 6: A 1 = 18.05, τ 1 = 15.11, A 2 = 4.15, τ 2 = 74.44, τ DW = 26.20.
    Figure Legend Snippet: Cells with fast initial decay kinetics express β 1 subunit mRNA A and B , scatter plots of IPSC 90%-width versus peak current amplitude for two RTN cells recorded on the same day. Each point in A and B represents an individual IPSC. A is a recording from a cell dominated with slow and small IPSCs (○) and B , represents a recording dominated by brief and large IPSCs ( ). C , ensemble averaged IPSCs averaged from isolated spontaneous inhibitory events (selected on the basis of having arisen from an event-free baseline and showing complete decay to the same baseline) for the two cells represented in A (black, n = 258) and B (grey, n = 217). Each trace was best fitted to two exponentials (dotted line superimposed on each trace). Note: the cell dominated with fast IPSCs yielded a ensemble averaged IPSC with a larger A 1 value than A 2 whereas the opposite is true for the slower cell. D , ethidium bromide stained 8% polyacrylamide gel showing single-cell RT-PCR analysis from five RTN cells examined in one day of recording, two of the cells are represented in A (lane 3) and B (lane 7). Cells positive for the β 1 subunit cDNA showed a solitary band at 162 bp after two rounds of amplification using nested primers (lanes 6 and 7, see methods). Lanes 1 and 2 are water and media controls that underwent reverse transcription along with individual cells in lanes 3 through 7; lane 8 is a positive control using 40 pg of total RNA; lanes 9 and 10 are water controls used in the master mix for each round of PCR amplification. The molecular weight marker in lane 11 is the Msp I digest of pBR322. The size of each fragment is noted on the right. The parameters for β 1 negative cells in lane 4: A 1 = 9.80, τ 1 = 13.75, A 2 = 14.17, τ 2 = 64.61, τ DW = 43.82l; and lane 5: A 1 = 12.94, τ 1 = 12.94, A 2 = 5.69, τ 2 = 77.21, τ DW = 29.63. The β 1 positive cell in lane 6: A 1 = 18.05, τ 1 = 15.11, A 2 = 4.15, τ 2 = 74.44, τ DW = 26.20.

    Techniques Used: Isolation, Staining, Reverse Transcription Polymerase Chain Reaction, Amplification, Positive Control, Polymerase Chain Reaction, Molecular Weight, Marker

    Related Articles

    Clone Assay:

    Article Title: Characterization of the Genes Encoding d-Amino Acid Transaminase and Glutamate Racemase, Two d-Glutamate Biosynthetic Enzymes of Bacillus sphaericus ATCC 10208
    Article Snippet: Paragraph title: Cloning of d -glutamate biosynthetic genes from B. sphaericus. ... Where appropriate, chloramphenicol (10 μg/ml) and ampicillin (200 μg/ml) were added. pBR322 DNA was obtained from New England Biolabs (Beverly, Mass.).

    Amplification:

    Article Title: Nontuberculous Mycobacterial Lung Disease Caused by Mycobacterium chelonae: A Case Report
    Article Snippet: Amplification of the partial rpoB gene (360 bp) was performed using primers Rpo5' (5'-TCAAGGAGAAGCGCTACGA-3') and Rpo3' (5'-GGATGTTGATCAGGGTCTGC-3') with subsequent digestion with 5 units of Msp I (New England BioLabs, Beverly, MA, USA) for 3 hours at 37℃. .. DNA size markers, pBR322-Msp I-digested DNA (New England BioLabs) were used to enable estimation of DNA fragment size.

    Article Title: NeuroD1 Gene and Interleukin-18 Gene Polymorphisms in Type 1 Diabetes in the Dalmatian Population of Southern Croatia
    Article Snippet: For determination of fragment length DNA marker, pBR322 DNA-MspI Digest (New England BioLabs) was used. .. A control forward primer (-137 CTRL; 5′ – CCAATAGGACTGATTATTCCGCA- 3′) was used to amplify a 446-bp fragment covering the polymorphic site as an internal positive amplification control.

    Article Title: Fast IPSCs in rat thalamic reticular nucleus require the GABAA receptor ?1 subunit
    Article Snippet: Amplification products were analysed on either a 2% agarose gel or an 8% polyacrylamide gel and always yielded a single ethidium bromide stained-band that migrated at the predicted molecular weight of 162 bp. .. The molecular weight marker was the pBR322 DNA– Msp I digest (New England Biolabs, Ipswich, MA, USA).

    Expressing:

    Article Title: The Epstein-Barr virus lytic program is controlled by the co-operative functions of two transactivators
    Article Snippet: The labeled oligonucleotide was purified on a 10% acrylamide gel and annealed with mRNAs from the B95.8 cell line, from 293 cells transfected with an expression plasmid coding for BRLF1 or from 293-BRLF1-KO cells transfected with an expression plasmid coding for BRLF1. .. Msp I-digested pBR322 DNA (New England Biolabs, MA) was radiolabeled and used as a DNA molecular weight reference.

    Positive Control:

    Article Title: Goniothalamin-induced oxidative stress, DNA damage and apoptosis via caspase-2 independent and Bcl-2 independent pathways in Jurkat T-cells
    Article Snippet: Topoisomerase II enzyme, GTN, and etoposide as a positive control, at the indicated concentrations were incubated in the buffer on ice for 10 min. .. Reactions contained 6 units (∼13 nM) of human topoisomerase IIα (TopoGen) and 300 ng of pBR322 DNA (New England Biolabs) per 20 μl sample volume.

    Nuclear Magnetic Resonance:

    Article Title: Mechanistic Studies of the Anticancer Activity of An Octahedral Hexanuclear Pt(II) Cage
    Article Snippet: Calf-thymus DNA was purchased from Invitrogen, pBR322 plamid DNA from New England Biolabs and deuterated solvents were purchased from Cambridge Isotope Laboratory (Andover, MA). .. 1 H NMR and DOSY NMR spectra were recorded on a Bruker AVANCE-400 NMR spectrometer with a Spectro Spin superconducting magnet in the Massachusetts Institute of Technology Department of Chemistry Instrumentation Facility (MIT DCIF).

    Activity Assay:

    Article Title: Goniothalamin-induced oxidative stress, DNA damage and apoptosis via caspase-2 independent and Bcl-2 independent pathways in Jurkat T-cells
    Article Snippet: Reactions contained 6 units (∼13 nM) of human topoisomerase IIα (TopoGen) and 300 ng of pBR322 DNA (New England Biolabs) per 20 μl sample volume. .. Following 10 min incubation, the enzyme activity was terminated and cleavage products were trapped by the addition of 2 μl of 10% SDS followed by the addition of EDTA and NaCl to 10 mM and 20 mM, respectively.

    Mass Spectrometry:

    Article Title: Mechanistic Studies of the Anticancer Activity of An Octahedral Hexanuclear Pt(II) Cage
    Article Snippet: Calf-thymus DNA was purchased from Invitrogen, pBR322 plamid DNA from New England Biolabs and deuterated solvents were purchased from Cambridge Isotope Laboratory (Andover, MA). .. Electrospray ionization mass spectrometry (ESI-MS) spectra were obtained on an Agilent Technologies 1100 Series liquid chromatography/MS instrument.

    Acrylamide Gel Assay:

    Article Title: The Epstein-Barr virus lytic program is controlled by the co-operative functions of two transactivators
    Article Snippet: After reverse transcription with Superscript™II Reverse Transcriptase (Life Technologies, Eggenstein, Germany) and addition of RNase A, the samples were separated on an 8% denaturing acrylamide gel. .. Msp I-digested pBR322 DNA (New England Biolabs, MA) was radiolabeled and used as a DNA molecular weight reference.

    Derivative Assay:

    Article Title: Characterization of the Genes Encoding d-Amino Acid Transaminase and Glutamate Racemase, Two d-Glutamate Biosynthetic Enzymes of Bacillus sphaericus ATCC 10208
    Article Snippet: Where appropriate, chloramphenicol (10 μg/ml) and ampicillin (200 μg/ml) were added. pBR322 DNA was obtained from New England Biolabs (Beverly, Mass.). .. Genes inserted between the Bam HI and Bgl II sites are transcribed from a strong constitutive promoter ( ) derived from the pheA ( ) promoter of E. coli K-12, located immediately upstream between the unique Hin dIII and Bam HI sites of the vector.

    Transfection:

    Article Title: The Epstein-Barr virus lytic program is controlled by the co-operative functions of two transactivators
    Article Snippet: The labeled oligonucleotide was purified on a 10% acrylamide gel and annealed with mRNAs from the B95.8 cell line, from 293 cells transfected with an expression plasmid coding for BRLF1 or from 293-BRLF1-KO cells transfected with an expression plasmid coding for BRLF1. .. Msp I-digested pBR322 DNA (New England Biolabs, MA) was radiolabeled and used as a DNA molecular weight reference.

    Sequencing:

    Article Title: NeuroD1 Gene and Interleukin-18 Gene Polymorphisms in Type 1 Diabetes in the Dalmatian Population of Southern Croatia
    Article Snippet: For determination of fragment length DNA marker, pBR322 DNA-MspI Digest (New England BioLabs) was used. .. IL-18 polymorphisms were detected by using PCR sequence-specific primers, according to the previous reports ( , ).

    Chromatography:

    Article Title: Mechanistic Studies of the Anticancer Activity of An Octahedral Hexanuclear Pt(II) Cage
    Article Snippet: Calf-thymus DNA was purchased from Invitrogen, pBR322 plamid DNA from New England Biolabs and deuterated solvents were purchased from Cambridge Isotope Laboratory (Andover, MA). .. Electrospray ionization mass spectrometry (ESI-MS) spectra were obtained on an Agilent Technologies 1100 Series liquid chromatography/MS instrument.

    Concentration Assay:

    Article Title: Goniothalamin-induced oxidative stress, DNA damage and apoptosis via caspase-2 independent and Bcl-2 independent pathways in Jurkat T-cells
    Article Snippet: Reactions contained 6 units (∼13 nM) of human topoisomerase IIα (TopoGen) and 300 ng of pBR322 DNA (New England Biolabs) per 20 μl sample volume. .. Enzyme trapped in cleavable complexes was removed by proteolysis with proteinase K at a final concentration of 0.8 mg/ml and incubated at 55 °C for 2 h to reveal any linearized DNA products.

    Article Title: Nucleoprotein complex intermediates in HIV-1 integration
    Article Snippet: 2 Mix and incubate on ice for 30 min. 3 Add: 0.5 μl 500 ng/μl pBR322 DNA (New England Biolabs) as the target DNA. .. 4 Incubate on ice for 30 min. 5 Transfer the reaction mixture to 37 °C and incubate for 2 h. 6 Stop the reaction by adding EDTA to the final concentration of 10 mM.

    Transferring:

    Article Title: Fast IPSCs in rat thalamic reticular nucleus require the GABAA receptor ?1 subunit
    Article Snippet: In a 50-μl reaction the ingredients were as follows: 1 × PCR buffer, 0.2 m m dNTP mixture (each), 1.5 m m MgCl2 , 20 pmol μl−1 of the outer primer pair, nuclease free water, the contents of the cDNA template reaction from a single cell (or the contents of cDNA reactions done in the presence of water, pipette solution and total RNA controls) and 2.5 units of DNA polymerase (Platinum Taq , Invitrogen Life Technologies). .. The molecular weight marker was the pBR322 DNA– Msp I digest (New England Biolabs, Ipswich, MA, USA).

    other:

    Article Title: Cylindrical Illumination Confocal Spectroscopy: Rectifying the Limitations of Confocal Single Molecule Spectroscopy through One-Dimensional Beam Shaping
    Article Snippet: Illumination powers of 1.85 mW/cm2 and 0.057 mW/cm2 were used for CICS and SMD analysis of pBR322 DNA, respectively.

    Article Title: Cylindrical Illumination Confocal Spectroscopy: Rectifying the Limitations of Confocal Single Molecule Spectroscopy through One-Dimensional Beam Shaping
    Article Snippet: For 488-CICS analysis of pBR322 DNA, the cylindrical lens is inserted into the beam path, and the circular pinhole is swapped for a 620 × 115 μ m rectangular confocal aperture.

    Negative Control:

    Article Title: Fast IPSCs in rat thalamic reticular nucleus require the GABAA receptor ?1 subunit
    Article Snippet: The molecular weight marker was the pBR322 DNA– Msp I digest (New England Biolabs, Ipswich, MA, USA). .. When a negative control was positive, then the entire round of PCR reactions (and thus entire day of recordings) were eliminated from the study.

    Polymerase Chain Reaction:

    Article Title: NeuroD1 Gene and Interleukin-18 Gene Polymorphisms in Type 1 Diabetes in the Dalmatian Population of Southern Croatia
    Article Snippet: For determination of fragment length DNA marker, pBR322 DNA-MspI Digest (New England BioLabs) was used. .. IL-18 polymorphisms were detected by using PCR sequence-specific primers, according to the previous reports ( , ).

    Article Title: Fetal Mouse Cyp1b1 and Transplacental Carcinogenesis from Maternal Exposure to Dibenzo[a,l]pyrene
    Article Snippet: Cyp1b1 heterozygotes yielded two PCR products of 365-bp and 460-bp (NEO), respectively. .. A molecular weight ladder of Msp I cut pBR322 DNA (New England Biolabs, Ipswich, MA) and ethidium bromide (Sigma Chemical Co., St. Louis, MO) was used to stain the DNA, followed by UV visualization.

    Molecular Weight:

    Article Title: The Epstein-Barr virus lytic program is controlled by the co-operative functions of two transactivators
    Article Snippet: .. Msp I-digested pBR322 DNA (New England Biolabs, MA) was radiolabeled and used as a DNA molecular weight reference. .. We thank B.Neuhierl for helpful discussions and critical reading of the manuscript.

    Article Title: Fast IPSCs in rat thalamic reticular nucleus require the GABAA receptor ?1 subunit
    Article Snippet: .. The molecular weight marker was the pBR322 DNA– Msp I digest (New England Biolabs, Ipswich, MA, USA). ..

    Article Title: Fetal Mouse Cyp1b1 and Transplacental Carcinogenesis from Maternal Exposure to Dibenzo[a,l]pyrene
    Article Snippet: .. A molecular weight ladder of Msp I cut pBR322 DNA (New England Biolabs, Ipswich, MA) and ethidium bromide (Sigma Chemical Co., St. Louis, MO) was used to stain the DNA, followed by UV visualization. .. Litter sizes per dam were compared between treatments using the exact Kruskal-Wallis test (SAS 9.1.3 Npar1way procedure).

    Nucleic Acid Electrophoresis:

    Article Title: Nucleoprotein complex intermediates in HIV-1 integration
    Article Snippet: 2 Mix and incubate on ice for 30 min. 3 Add: 0.5 μl 500 ng/μl pBR322 DNA (New England Biolabs) as the target DNA. .. The BSA helps to prevent non-specific interaction of the complexes with agarose during gel electrophoresis.

    Mutagenesis:

    Article Title: Locus-specific control of the de novo DNA methylation pathway in Arabidopsis by the CLASSY family
    Article Snippet: Small RNA isolation: 4 un-opened flower buds (stage 12 and younger) from individual mutant plants as well as 3 individual wild-type (WT) controls were collected, frozen in liquid nitrogen and kept at −80°C until use. .. The final library products were further purified using an 8% polyacrylamide gel to excise 130–160nt products relative to the pBR322 DNA-MspI Digest ladder (New England Biolabs, Cat# E7323AA).

    Article Title: Mammalian NET-seq analysis defines nascent RNA profiles and associated RNA processing genome-wide
    Article Snippet: MgCl2 , 1 M (Sigma-Aldrich, cat. no. M1028) NaCl (VWR Chemicals, cat. no. 27810.295) NP-40, IGEPAL CA630 (Sigma, cat. no. I8896) Nuclease-free water, not DEPC-Treated (Life technologies, cat. no. AM9937) pBR322 MspI digest (NEB, cat. no. N3032S) γ-32 P ATP (Perkin Elmer, 6,000 Ci/mmol, cat. no. NEG502Z) ! .. PhosSTOP, phosphatase inhibitor cocktail (Roche, cat. no. 04906837001) Appropriate RNA polymerase II antibodies ( ) RNaseZap (Ambion, cat. no. AM9782) Sodium acetate (VWR Chemicals, cat. no. 27652.260) SuperScript III reverse transcriptase (Life technologies, cat. no. 18080-044) T4 polynucleotide kinase (PNK), 3′ phosphatase minus (NEB, cat. no. M0236S) T4 RNA ligase, deletion mutant 2 (Epicentre, cat. no. LR2D1132K) Tris, Trizmabase (Sigma-Aldrich, cat. no. T6066) TRIzol reagent (Life Technologies, cat. no. 15596-026) !

    Isolation:

    Article Title: Nontuberculous Mycobacterial Lung Disease Caused by Mycobacterium chelonae: A Case Report
    Article Snippet: NTM were isolated three times from sputum specimens. .. DNA size markers, pBR322-Msp I-digested DNA (New England BioLabs) were used to enable estimation of DNA fragment size.

    Article Title: Locus-specific control of the de novo DNA methylation pathway in Arabidopsis by the CLASSY family
    Article Snippet: Paragraph title: Small RNA isolation: ... The final library products were further purified using an 8% polyacrylamide gel to excise 130–160nt products relative to the pBR322 DNA-MspI Digest ladder (New England Biolabs, Cat# E7323AA).

    Silver Staining:

    Article Title: NeuroD1 Gene and Interleukin-18 Gene Polymorphisms in Type 1 Diabetes in the Dalmatian Population of Southern Croatia
    Article Snippet: The digested fragments were separated on 10% polyacrylamide gels and visualized by silver staining ( ). .. For determination of fragment length DNA marker, pBR322 DNA-MspI Digest (New England BioLabs) was used.

    RNA Extraction:

    Article Title: Locus-specific control of the de novo DNA methylation pathway in Arabidopsis by the CLASSY family
    Article Snippet: The total RNA extraction and small RNA enrichment were performed as previously described with the following minor modifications: (1) for the small RNA enrichment step an equal volume of 20% polyethylene glycol 8000/2M NaCl was added to each total RNA sample and (2) the ZR-small RNA ladder (Zymo Research, Cat# R1090) was used to determine the gel region corresponding to the 17–29 nucleotide (nt) size range. .. The final library products were further purified using an 8% polyacrylamide gel to excise 130–160nt products relative to the pBR322 DNA-MspI Digest ladder (New England Biolabs, Cat# E7323AA).

    Labeling:

    Article Title: The Epstein-Barr virus lytic program is controlled by the co-operative functions of two transactivators
    Article Snippet: The labeled oligonucleotide was purified on a 10% acrylamide gel and annealed with mRNAs from the B95.8 cell line, from 293 cells transfected with an expression plasmid coding for BRLF1 or from 293-BRLF1-KO cells transfected with an expression plasmid coding for BRLF1. .. Msp I-digested pBR322 DNA (New England Biolabs, MA) was radiolabeled and used as a DNA molecular weight reference.

    Mouse Assay:

    Article Title: Fetal Mouse Cyp1b1 and Transplacental Carcinogenesis from Maternal Exposure to Dibenzo[a,l]pyrene
    Article Snippet: Paragraph title: Genotyping of mice for the Cyp1b1 allele ... A molecular weight ladder of Msp I cut pBR322 DNA (New England Biolabs, Ipswich, MA) and ethidium bromide (Sigma Chemical Co., St. Louis, MO) was used to stain the DNA, followed by UV visualization.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Fast IPSCs in rat thalamic reticular nucleus require the GABAA receptor ?1 subunit
    Article Snippet: Paragraph title: Single-cell RT-PCR ... The molecular weight marker was the pBR322 DNA– Msp I digest (New England Biolabs, Ipswich, MA, USA).

    Purification:

    Article Title: Mechanistic Studies of the Anticancer Activity of An Octahedral Hexanuclear Pt(II) Cage
    Article Snippet: The compounds Pt(bpy)Cl2 and 2 were prepared according to previous reports., All reagents were purchased from Strem, Aldrich, or Alfa and used without further purification under normal atmospheric conditions. .. Calf-thymus DNA was purchased from Invitrogen, pBR322 plamid DNA from New England Biolabs and deuterated solvents were purchased from Cambridge Isotope Laboratory (Andover, MA).

    Article Title: The Epstein-Barr virus lytic program is controlled by the co-operative functions of two transactivators
    Article Snippet: The labeled oligonucleotide was purified on a 10% acrylamide gel and annealed with mRNAs from the B95.8 cell line, from 293 cells transfected with an expression plasmid coding for BRLF1 or from 293-BRLF1-KO cells transfected with an expression plasmid coding for BRLF1. .. Msp I-digested pBR322 DNA (New England Biolabs, MA) was radiolabeled and used as a DNA molecular weight reference.

    Article Title: Locus-specific control of the de novo DNA methylation pathway in Arabidopsis by the CLASSY family
    Article Snippet: .. The final library products were further purified using an 8% polyacrylamide gel to excise 130–160nt products relative to the pBR322 DNA-MspI Digest ladder (New England Biolabs, Cat# E7323AA). .. The libraries were pooled and sequenced (single end 50bp, SE50) on a HiSeq 2500 machine (Illumina).

    Plasmid Preparation:

    Article Title: Goniothalamin-induced oxidative stress, DNA damage and apoptosis via caspase-2 independent and Bcl-2 independent pathways in Jurkat T-cells
    Article Snippet: Reactions were initiated by the addition of pBR322 plasmid DNA and transferred to a 37 °C water bath. .. Reactions contained 6 units (∼13 nM) of human topoisomerase IIα (TopoGen) and 300 ng of pBR322 DNA (New England Biolabs) per 20 μl sample volume.

    Article Title: Characterization of the Genes Encoding d-Amino Acid Transaminase and Glutamate Racemase, Two d-Glutamate Biosynthetic Enzymes of Bacillus sphaericus ATCC 10208
    Article Snippet: Where appropriate, chloramphenicol (10 μg/ml) and ampicillin (200 μg/ml) were added. pBR322 DNA was obtained from New England Biolabs (Beverly, Mass.). .. Plasmid pIF306 is a pBR322 ( ) derivative containing a unique Bgl II site inserted between the vector Bam HI and Sph I sites.

    Article Title: The Epstein-Barr virus lytic program is controlled by the co-operative functions of two transactivators
    Article Snippet: The labeled oligonucleotide was purified on a 10% acrylamide gel and annealed with mRNAs from the B95.8 cell line, from 293 cells transfected with an expression plasmid coding for BRLF1 or from 293-BRLF1-KO cells transfected with an expression plasmid coding for BRLF1. .. Msp I-digested pBR322 DNA (New England Biolabs, MA) was radiolabeled and used as a DNA molecular weight reference.

    Multiplex Assay:

    Article Title: Locus-specific control of the de novo DNA methylation pathway in Arabidopsis by the CLASSY family
    Article Snippet: The resulting small RNAs were then used for library preparation with the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, Cat# E7300) following the user’s manual. .. The final library products were further purified using an 8% polyacrylamide gel to excise 130–160nt products relative to the pBR322 DNA-MspI Digest ladder (New England Biolabs, Cat# E7323AA).

    Agarose Gel Electrophoresis:

    Article Title: Characterization of the Genes Encoding d-Amino Acid Transaminase and Glutamate Racemase, Two d-Glutamate Biosynthetic Enzymes of Bacillus sphaericus ATCC 10208
    Article Snippet: Where appropriate, chloramphenicol (10 μg/ml) and ampicillin (200 μg/ml) were added. pBR322 DNA was obtained from New England Biolabs (Beverly, Mass.). .. Partially Mbo I-digested chromosomal DNA ( ) of B. sphaericus ATCC 10208 was size fractionated on a 0.8% agarose gel, and 2- to 10-kb fragments were ligated to Bam HI- and Bgl II-cleaved pIF306.

    Article Title: Fast IPSCs in rat thalamic reticular nucleus require the GABAA receptor ?1 subunit
    Article Snippet: Amplification products were analysed on either a 2% agarose gel or an 8% polyacrylamide gel and always yielded a single ethidium bromide stained-band that migrated at the predicted molecular weight of 162 bp. .. The molecular weight marker was the pBR322 DNA– Msp I digest (New England Biolabs, Ipswich, MA, USA).

    Size-exclusion Chromatography:

    Article Title: Fetal Mouse Cyp1b1 and Transplacental Carcinogenesis from Maternal Exposure to Dibenzo[a,l]pyrene
    Article Snippet: PCR cycling conditions were an initial 5 min 95°C enzyme activation step, followed by 35 cycles of: 30 sec at 95°C to denature the DNA; 30 sec at 55°C for primer annealing; 45 sec at 72°C for extension. .. A molecular weight ladder of Msp I cut pBR322 DNA (New England Biolabs, Ipswich, MA) and ethidium bromide (Sigma Chemical Co., St. Louis, MO) was used to stain the DNA, followed by UV visualization.

    Incubation:

    Article Title: Goniothalamin-induced oxidative stress, DNA damage and apoptosis via caspase-2 independent and Bcl-2 independent pathways in Jurkat T-cells
    Article Snippet: Topoisomerase II enzyme, GTN, and etoposide as a positive control, at the indicated concentrations were incubated in the buffer on ice for 10 min. .. Reactions contained 6 units (∼13 nM) of human topoisomerase IIα (TopoGen) and 300 ng of pBR322 DNA (New England Biolabs) per 20 μl sample volume.

    Produced:

    Article Title: NeuroD1 Gene and Interleukin-18 Gene Polymorphisms in Type 1 Diabetes in the Dalmatian Population of Southern Croatia
    Article Snippet: Homozygotes AA (Thr/Thr) produced two fragments, while the homozygote GG (Ala/Ala) produced one 198-bp fragment. .. For determination of fragment length DNA marker, pBR322 DNA-MspI Digest (New England BioLabs) was used.

    Activation Assay:

    Article Title: Fetal Mouse Cyp1b1 and Transplacental Carcinogenesis from Maternal Exposure to Dibenzo[a,l]pyrene
    Article Snippet: PCR cycling conditions were an initial 5 min 95°C enzyme activation step, followed by 35 cycles of: 30 sec at 95°C to denature the DNA; 30 sec at 55°C for primer annealing; 45 sec at 72°C for extension. .. A molecular weight ladder of Msp I cut pBR322 DNA (New England Biolabs, Ipswich, MA) and ethidium bromide (Sigma Chemical Co., St. Louis, MO) was used to stain the DNA, followed by UV visualization.

    Marker:

    Article Title: NeuroD1 Gene and Interleukin-18 Gene Polymorphisms in Type 1 Diabetes in the Dalmatian Population of Southern Croatia
    Article Snippet: .. For determination of fragment length DNA marker, pBR322 DNA-MspI Digest (New England BioLabs) was used. .. IL-18 polymorphisms were detected by using PCR sequence-specific primers, according to the previous reports ( , ).

    Article Title: Fast IPSCs in rat thalamic reticular nucleus require the GABAA receptor ?1 subunit
    Article Snippet: .. The molecular weight marker was the pBR322 DNA– Msp I digest (New England Biolabs, Ipswich, MA, USA). ..

    Staining:

    Article Title: Cylindrical Illumination Confocal Spectroscopy: Rectifying the Limitations of Confocal Single Molecule Spectroscopy through One-Dimensional Beam Shaping
    Article Snippet: .. For 488-SMD and 488-CICS analysis, pBR322 DNA (4.3 kbp; New England BioLabs, Ipswich, MA) was stained with PicoGreen (Invitrogen) using the protocol developed by Yan et al. ( ). .. The DNA was diluted to100 ng/mL in TE buffer and stained with 1 μ M PicoGreen for 1 h in the dark.

    Article Title: Fast IPSCs in rat thalamic reticular nucleus require the GABAA receptor ?1 subunit
    Article Snippet: Amplification products were analysed on either a 2% agarose gel or an 8% polyacrylamide gel and always yielded a single ethidium bromide stained-band that migrated at the predicted molecular weight of 162 bp. .. The molecular weight marker was the pBR322 DNA– Msp I digest (New England Biolabs, Ipswich, MA, USA).

    Article Title: Fetal Mouse Cyp1b1 and Transplacental Carcinogenesis from Maternal Exposure to Dibenzo[a,l]pyrene
    Article Snippet: .. A molecular weight ladder of Msp I cut pBR322 DNA (New England Biolabs, Ipswich, MA) and ethidium bromide (Sigma Chemical Co., St. Louis, MO) was used to stain the DNA, followed by UV visualization. .. Litter sizes per dam were compared between treatments using the exact Kruskal-Wallis test (SAS 9.1.3 Npar1way procedure).

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  • 94
    New England Biolabs pbr322 dna msp
    Cells with fast initial decay kinetics express β 1 subunit mRNA A and B , scatter plots of IPSC 90%-width versus peak current amplitude for two RTN cells recorded on the same day. Each point in A and B represents an individual IPSC. A is a recording from a cell dominated with slow and small IPSCs (○) and B , represents a recording dominated by brief and large IPSCs ( ). C , ensemble averaged IPSCs averaged from isolated spontaneous inhibitory events (selected on the basis of having arisen from an event-free baseline and showing complete decay to the same baseline) for the two cells represented in A (black, n = 258) and B (grey, n = 217). Each trace was best fitted to two exponentials (dotted line superimposed on each trace). Note: the cell dominated with fast IPSCs yielded a ensemble averaged IPSC with a larger A 1 value than A 2 whereas the opposite is true for the slower cell. D , ethidium bromide stained 8% polyacrylamide gel showing single-cell RT-PCR analysis from five RTN cells examined in one day of recording, two of the cells are represented in A (lane 3) and B (lane 7). Cells positive for the β 1 subunit cDNA showed a solitary band at 162 bp after two rounds of amplification using nested primers (lanes 6 and 7, see methods). Lanes 1 and 2 are water and media controls that underwent reverse transcription along with individual cells in lanes 3 through 7; lane 8 is a positive control using 40 pg of total RNA; lanes 9 and 10 are water controls used in the master mix for each round of PCR amplification. The molecular weight marker in lane 11 is the <t>Msp</t> I digest of <t>pBR322.</t> The size of each fragment is noted on the right. The parameters for β 1 negative cells in lane 4: A 1 = 9.80, τ 1 = 13.75, A 2 = 14.17, τ 2 = 64.61, τ DW = 43.82l; and lane 5: A 1 = 12.94, τ 1 = 12.94, A 2 = 5.69, τ 2 = 77.21, τ DW = 29.63. The β 1 positive cell in lane 6: A 1 = 18.05, τ 1 = 15.11, A 2 = 4.15, τ 2 = 74.44, τ DW = 26.20.
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    Cells with fast initial decay kinetics express β 1 subunit mRNA A and B , scatter plots of IPSC 90%-width versus peak current amplitude for two RTN cells recorded on the same day. Each point in A and B represents an individual IPSC. A is a recording from a cell dominated with slow and small IPSCs (○) and B , represents a recording dominated by brief and large IPSCs ( ). C , ensemble averaged IPSCs averaged from isolated spontaneous inhibitory events (selected on the basis of having arisen from an event-free baseline and showing complete decay to the same baseline) for the two cells represented in A (black, n = 258) and B (grey, n = 217). Each trace was best fitted to two exponentials (dotted line superimposed on each trace). Note: the cell dominated with fast IPSCs yielded a ensemble averaged IPSC with a larger A 1 value than A 2 whereas the opposite is true for the slower cell. D , ethidium bromide stained 8% polyacrylamide gel showing single-cell RT-PCR analysis from five RTN cells examined in one day of recording, two of the cells are represented in A (lane 3) and B (lane 7). Cells positive for the β 1 subunit cDNA showed a solitary band at 162 bp after two rounds of amplification using nested primers (lanes 6 and 7, see methods). Lanes 1 and 2 are water and media controls that underwent reverse transcription along with individual cells in lanes 3 through 7; lane 8 is a positive control using 40 pg of total RNA; lanes 9 and 10 are water controls used in the master mix for each round of PCR amplification. The molecular weight marker in lane 11 is the Msp I digest of pBR322. The size of each fragment is noted on the right. The parameters for β 1 negative cells in lane 4: A 1 = 9.80, τ 1 = 13.75, A 2 = 14.17, τ 2 = 64.61, τ DW = 43.82l; and lane 5: A 1 = 12.94, τ 1 = 12.94, A 2 = 5.69, τ 2 = 77.21, τ DW = 29.63. The β 1 positive cell in lane 6: A 1 = 18.05, τ 1 = 15.11, A 2 = 4.15, τ 2 = 74.44, τ DW = 26.20.

    Journal: The Journal of Physiology

    Article Title: Fast IPSCs in rat thalamic reticular nucleus require the GABAA receptor ?1 subunit

    doi: 10.1113/jphysiol.2006.106617

    Figure Lengend Snippet: Cells with fast initial decay kinetics express β 1 subunit mRNA A and B , scatter plots of IPSC 90%-width versus peak current amplitude for two RTN cells recorded on the same day. Each point in A and B represents an individual IPSC. A is a recording from a cell dominated with slow and small IPSCs (○) and B , represents a recording dominated by brief and large IPSCs ( ). C , ensemble averaged IPSCs averaged from isolated spontaneous inhibitory events (selected on the basis of having arisen from an event-free baseline and showing complete decay to the same baseline) for the two cells represented in A (black, n = 258) and B (grey, n = 217). Each trace was best fitted to two exponentials (dotted line superimposed on each trace). Note: the cell dominated with fast IPSCs yielded a ensemble averaged IPSC with a larger A 1 value than A 2 whereas the opposite is true for the slower cell. D , ethidium bromide stained 8% polyacrylamide gel showing single-cell RT-PCR analysis from five RTN cells examined in one day of recording, two of the cells are represented in A (lane 3) and B (lane 7). Cells positive for the β 1 subunit cDNA showed a solitary band at 162 bp after two rounds of amplification using nested primers (lanes 6 and 7, see methods). Lanes 1 and 2 are water and media controls that underwent reverse transcription along with individual cells in lanes 3 through 7; lane 8 is a positive control using 40 pg of total RNA; lanes 9 and 10 are water controls used in the master mix for each round of PCR amplification. The molecular weight marker in lane 11 is the Msp I digest of pBR322. The size of each fragment is noted on the right. The parameters for β 1 negative cells in lane 4: A 1 = 9.80, τ 1 = 13.75, A 2 = 14.17, τ 2 = 64.61, τ DW = 43.82l; and lane 5: A 1 = 12.94, τ 1 = 12.94, A 2 = 5.69, τ 2 = 77.21, τ DW = 29.63. The β 1 positive cell in lane 6: A 1 = 18.05, τ 1 = 15.11, A 2 = 4.15, τ 2 = 74.44, τ DW = 26.20.

    Article Snippet: The molecular weight marker was the pBR322 DNA– Msp I digest (New England Biolabs, Ipswich, MA, USA).

    Techniques: Isolation, Staining, Reverse Transcription Polymerase Chain Reaction, Amplification, Positive Control, Polymerase Chain Reaction, Molecular Weight, Marker