restriction enzyme mspi  (New England Biolabs)


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    Structured Review

    New England Biolabs restriction enzyme mspi
    Restriction Enzyme Mspi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzyme mspi/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    restriction enzyme mspi - by Bioz Stars, 2023-02
    86/100 stars

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    pbr322 mspi  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
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    Structured Review

    New England Biolabs pbr322 mspi
    Recombinant mouse GST-Ago2 and let-7a form active RISC in vitro . 15 nM of either wild-type or catalytically inactive (D669A) GST-Ago2 was loaded with 0.02 μM synthetic 5′P-let-7a and then incubated with 13.6 nmol 5′- 32 P target RNA (TD). Reactions were analyzed by 15% Urea PAGE and detected by autoradiography. <t>pBR322/MspI</t> DNA marker (M) and a synthetic 10 nt oligo (G10) were radiolabeled and included as size markers.
    Pbr322 Mspi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbr322 mspi/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbr322 mspi - by Bioz Stars, 2023-02
    86/100 stars

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    1) Product Images from "Expanded RNA-binding activities of mammalian Argonaute 2"

    Article Title: Expanded RNA-binding activities of mammalian Argonaute 2

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkp812

    Recombinant mouse GST-Ago2 and let-7a form active RISC in vitro . 15 nM of either wild-type or catalytically inactive (D669A) GST-Ago2 was loaded with 0.02 μM synthetic 5′P-let-7a and then incubated with 13.6 nmol 5′- 32 P target RNA (TD). Reactions were analyzed by 15% Urea PAGE and detected by autoradiography. pBR322/MspI DNA marker (M) and a synthetic 10 nt oligo (G10) were radiolabeled and included as size markers.
    Figure Legend Snippet: Recombinant mouse GST-Ago2 and let-7a form active RISC in vitro . 15 nM of either wild-type or catalytically inactive (D669A) GST-Ago2 was loaded with 0.02 μM synthetic 5′P-let-7a and then incubated with 13.6 nmol 5′- 32 P target RNA (TD). Reactions were analyzed by 15% Urea PAGE and detected by autoradiography. pBR322/MspI DNA marker (M) and a synthetic 10 nt oligo (G10) were radiolabeled and included as size markers.

    Techniques Used: Recombinant, In Vitro, Incubation, Autoradiography, Marker