phi x 174 dna hae iii mix molecular weight marker  (New England Biolabs)


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    New England Biolabs phi x 174 dna hae iii mix molecular weight marker
    LTBP2 gene sequencing and mRNA analysis. ( a ) Family 1. Top to bottom, direct sequencing of genomic <t>DNA</t> from father, proband, and an ethnically matched unrelated control, showing c.1796_1797insC (p.Val600GlyfsX2) mutation in exon 9 of LTBP2 , heterozygous in father and homozygous in proband. ( b ) Family 2. Top to bottom, direct sequencing of genomic DNA from father, proband, and an ethnically matched unrelated control, showing c.895C > T (p.Arg299X) mutation in exon 4 of LTBP2 , heterozygous in father and homozygous in proband. ( c ) Analysis of LTBP2 transcript in cultured fibroblasts using primers situated in exons 7 and 12. 1–3: RT-PCR of three aliquots of mRNA extracted from fibroblasts culture of Family 1's proband; 4–5: RT-PCR of two unrelated control fibroblast cultures, showing a band of expected size (625 bp); 6: Negative control with H2O used in place of mRNA template; 7: Lambda DNA/ Hin d <t>III</t> and Phi X 174 DNA/ Hae III Mix molecular weight marker.
    Phi X 174 Dna Hae Iii Mix Molecular Weight Marker, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phi x 174 dna hae iii mix molecular weight marker/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phi x 174 dna hae iii mix molecular weight marker - by Bioz Stars, 2022-07
    86/100 stars

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    1) Product Images from "LTBP2 null mutations in an autosomal recessive ocular syndrome with megalocornea, spherophakia, and secondary glaucoma"

    Article Title: LTBP2 null mutations in an autosomal recessive ocular syndrome with megalocornea, spherophakia, and secondary glaucoma

    Journal: European Journal of Human Genetics

    doi: 10.1038/ejhg.2010.11

    LTBP2 gene sequencing and mRNA analysis. ( a ) Family 1. Top to bottom, direct sequencing of genomic DNA from father, proband, and an ethnically matched unrelated control, showing c.1796_1797insC (p.Val600GlyfsX2) mutation in exon 9 of LTBP2 , heterozygous in father and homozygous in proband. ( b ) Family 2. Top to bottom, direct sequencing of genomic DNA from father, proband, and an ethnically matched unrelated control, showing c.895C > T (p.Arg299X) mutation in exon 4 of LTBP2 , heterozygous in father and homozygous in proband. ( c ) Analysis of LTBP2 transcript in cultured fibroblasts using primers situated in exons 7 and 12. 1–3: RT-PCR of three aliquots of mRNA extracted from fibroblasts culture of Family 1's proband; 4–5: RT-PCR of two unrelated control fibroblast cultures, showing a band of expected size (625 bp); 6: Negative control with H2O used in place of mRNA template; 7: Lambda DNA/ Hin d III and Phi X 174 DNA/ Hae III Mix molecular weight marker.
    Figure Legend Snippet: LTBP2 gene sequencing and mRNA analysis. ( a ) Family 1. Top to bottom, direct sequencing of genomic DNA from father, proband, and an ethnically matched unrelated control, showing c.1796_1797insC (p.Val600GlyfsX2) mutation in exon 9 of LTBP2 , heterozygous in father and homozygous in proband. ( b ) Family 2. Top to bottom, direct sequencing of genomic DNA from father, proband, and an ethnically matched unrelated control, showing c.895C > T (p.Arg299X) mutation in exon 4 of LTBP2 , heterozygous in father and homozygous in proband. ( c ) Analysis of LTBP2 transcript in cultured fibroblasts using primers situated in exons 7 and 12. 1–3: RT-PCR of three aliquots of mRNA extracted from fibroblasts culture of Family 1's proband; 4–5: RT-PCR of two unrelated control fibroblast cultures, showing a band of expected size (625 bp); 6: Negative control with H2O used in place of mRNA template; 7: Lambda DNA/ Hin d III and Phi X 174 DNA/ Hae III Mix molecular weight marker.

    Techniques Used: Sequencing, Mutagenesis, Cell Culture, Reverse Transcription Polymerase Chain Reaction, Negative Control, Lambda DNA Preparation, Molecular Weight, Marker

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    New England Biolabs phi x 174 dna hae iii mix molecular weight marker
    LTBP2 gene sequencing and mRNA analysis. ( a ) Family 1. Top to bottom, direct sequencing of genomic <t>DNA</t> from father, proband, and an ethnically matched unrelated control, showing c.1796_1797insC (p.Val600GlyfsX2) mutation in exon 9 of LTBP2 , heterozygous in father and homozygous in proband. ( b ) Family 2. Top to bottom, direct sequencing of genomic DNA from father, proband, and an ethnically matched unrelated control, showing c.895C > T (p.Arg299X) mutation in exon 4 of LTBP2 , heterozygous in father and homozygous in proband. ( c ) Analysis of LTBP2 transcript in cultured fibroblasts using primers situated in exons 7 and 12. 1–3: RT-PCR of three aliquots of mRNA extracted from fibroblasts culture of Family 1's proband; 4–5: RT-PCR of two unrelated control fibroblast cultures, showing a band of expected size (625 bp); 6: Negative control with H2O used in place of mRNA template; 7: Lambda DNA/ Hin d <t>III</t> and Phi X 174 DNA/ Hae III Mix molecular weight marker.
    Phi X 174 Dna Hae Iii Mix Molecular Weight Marker, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phi x 174 dna hae iii mix molecular weight marker/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phi x 174 dna hae iii mix molecular weight marker - by Bioz Stars, 2022-07
    86/100 stars
      Buy from Supplier

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    LTBP2 gene sequencing and mRNA analysis. ( a ) Family 1. Top to bottom, direct sequencing of genomic DNA from father, proband, and an ethnically matched unrelated control, showing c.1796_1797insC (p.Val600GlyfsX2) mutation in exon 9 of LTBP2 , heterozygous in father and homozygous in proband. ( b ) Family 2. Top to bottom, direct sequencing of genomic DNA from father, proband, and an ethnically matched unrelated control, showing c.895C > T (p.Arg299X) mutation in exon 4 of LTBP2 , heterozygous in father and homozygous in proband. ( c ) Analysis of LTBP2 transcript in cultured fibroblasts using primers situated in exons 7 and 12. 1–3: RT-PCR of three aliquots of mRNA extracted from fibroblasts culture of Family 1's proband; 4–5: RT-PCR of two unrelated control fibroblast cultures, showing a band of expected size (625 bp); 6: Negative control with H2O used in place of mRNA template; 7: Lambda DNA/ Hin d III and Phi X 174 DNA/ Hae III Mix molecular weight marker.

    Journal: European Journal of Human Genetics

    Article Title: LTBP2 null mutations in an autosomal recessive ocular syndrome with megalocornea, spherophakia, and secondary glaucoma

    doi: 10.1038/ejhg.2010.11

    Figure Lengend Snippet: LTBP2 gene sequencing and mRNA analysis. ( a ) Family 1. Top to bottom, direct sequencing of genomic DNA from father, proband, and an ethnically matched unrelated control, showing c.1796_1797insC (p.Val600GlyfsX2) mutation in exon 9 of LTBP2 , heterozygous in father and homozygous in proband. ( b ) Family 2. Top to bottom, direct sequencing of genomic DNA from father, proband, and an ethnically matched unrelated control, showing c.895C > T (p.Arg299X) mutation in exon 4 of LTBP2 , heterozygous in father and homozygous in proband. ( c ) Analysis of LTBP2 transcript in cultured fibroblasts using primers situated in exons 7 and 12. 1–3: RT-PCR of three aliquots of mRNA extracted from fibroblasts culture of Family 1's proband; 4–5: RT-PCR of two unrelated control fibroblast cultures, showing a band of expected size (625 bp); 6: Negative control with H2O used in place of mRNA template; 7: Lambda DNA/ Hin d III and Phi X 174 DNA/ Hae III Mix molecular weight marker.

    Article Snippet: The amplified products were detected by running a 1% agarose gel with Lambda DNA/Hin d III and Phi X 174 DNA/Hae III Mix molecular weight marker (NEB).

    Techniques: Sequencing, Mutagenesis, Cell Culture, Reverse Transcription Polymerase Chain Reaction, Negative Control, Lambda DNA Preparation, Molecular Weight, Marker