n 6 methyladenine free bacteriophage lambda  (New England Biolabs)


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    Name:
    Lambda DNA N6 methyladenine free
    Description:
    Lambda DNA N6 methyladenine free 1 250 ug
    Catalog Number:
    n3013l
    Price:
    276
    Size:
    1 250 ug
    Category:
    Genomic DNA
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    New England Biolabs n 6 methyladenine free bacteriophage lambda
    Lambda DNA N6 methyladenine free
    Lambda DNA N6 methyladenine free 1 250 ug
    https://www.bioz.com/result/n 6 methyladenine free bacteriophage lambda/product/New England Biolabs
    Average 97 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    n 6 methyladenine free bacteriophage lambda - by Bioz Stars, 2020-09
    97/100 stars

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    Related Articles

    Sequencing:

    Article Title: Nanopore DNA Sequencing and Genome Assembly on the International Space Station
    Article Snippet: .. Library Preparation and Sequencing Samples analyzed in this study contained sequence libraries prepared from mixtures of female mouse BALB/C (Zyagen, San Diego, CA), Escherichia coli K-12 (Zyagen), and N 6-methyladenine-free bacteriophage lambda (New England Biolabs, Ipswich, MA) genomic DNA. .. The samples were aliquoted for library preparation on three platforms: Oxford Nanopore Technologies (ONT) MinION (v6), Illumina MiSeq (v2), and the Pacific Biosciences RSII.

    Methylation:

    Article Title: Fidelity Index Determination of DNA Methyltransferases
    Article Snippet: .. A standard methylation assay consisted of a 30 µL reaction containing a fixed concentration of [Methyl-3 H]-S -Adenosylmethionine (SAM, Perkin Elmer #NET155V001MC), 1 µg of N6 -methyladenine free λ DNA (NEB #N3013), and varying concentrations of methyltransferase from the two-fold dilution series (making up 10% of the final volume) in the appropriate methyltransferase buffer (see below). ..

    Article Title: Uncoupling histone H3K4 trimethylation from developmental gene expression via an equilibrium of COMPASS, Polycomb and DNA methylation.
    Article Snippet: .. The COMPASS protein family catalyzes histone H3 Lys 4 (H3K4) methylation and its members are essential for regulating gene expression. .. The COMPASS protein family catalyzes histone H3 Lys 4 (H3K4) methylation and its members are essential for regulating gene expression.

    Avidin-Biotin Assay:

    Article Title: Exploring protein-DNA interactions in 3D using in situ construction, manipulation, and visualization of individual DNA-dumbbells with optical traps, microfluidics, and fluorescence microscopy
    Article Snippet: .. 5-(3-aminoallyl) dUTP (Fermentas #R0091) Adenosine 5′-O-(3-thiotriphosphate) (ATPγS Calbiotech #119120) ATTO565 NHS-ester (ATTO-TEC GmbH #AD 565-31) Avidin agarose (Thermo Scientific #20219) Biotin-11-dGTP (PerkinElmer #NEL541) Bovine serum albumin (BSA, Sigma-Aldrich #A-9647) Deoxyribonucleoside triphosphates (dNTPs: Invitrogen dATP (#55082), TTP (#55085), dCTP (#55083) and dGTP (#55084) for PCR) Dithiothreitol (DTT Fisher Scientific #BP172-25) CAUTION causes eye and skin irritation; handle wearing goggles, lab coat and gloves Ethanol (EtOH Gold Shield Chemical Company #43196-117) CAUTION flammable; avoid open flames Ethylenediaminetetraacetic acid (EDTA, J. T. Baker #8993-01) Klenow Fragment DNA polymerase I (NEB #M0212S) Magnesium acetate (Mg(OAc)2 J. T. Baker #2424-01) Magnesium chloride (MgCl2 ) Methanol (MeOH Fisher Scientific #A412-4) CAUTION flammable; avoid open flames NEB buffer #2 (NEB buffer supplied with Klenow Fragment DNA polymerase I NEB #B7002S) Phage λ DNA (NEB #N3013S) Potassium hydroxide (KOH Sigma-Aldrich #221473-1KG) CAUTION corrosive; handle wearing goggles, lab coat and gloves Primers for polymerase chain reaction (PCR) to yield a 430 bp product identical to λ DNA between base pairs 23,788 and 24,217: forward primer 5′-biotin-ACTGTTCTTGCGGTTTGGAGG-3′ reverse primer 5′-CTATCGGAAGTTCACCAGCCAG-3′ (can be purchased from any high quality source such as Sigma, Integrated DNA Technologies, or Invitrogen) Sodium bicarbonate (NaHCO3 J. T. Baker #3506-05) Sodium chloride (NaCl) Sodium hydroxide (NaOH) Streptavidin-coated polystyrene beads, 1 μm (Bangs Laboratories #CP01N/10021) Sucrose (Sigma-Aldrich #S7903-5KG) ThermoPol buffer (NEB buffer supplied with VentR ® (exo− ) DNA polymerase NEB #B9004S) Tris acetate (TrisOAc TRIZMA base Sigma-Aldrich #T-1503 + Acetic acid EMD #AX0073-9) CAUTION Acetic acid is corrosive; handle wearing goggles, lab coat and gloves in ventilated hood Water, ultrapure Type 1 (Nanopure (Barnstead) or Milli-Q (Millipore)) VentR ® (exo− ) DNA Polymerase (NEB #M0257) YOYO-1 (Invitrogen #Y3601) .. Abrasive blasting cabinet (Harbor Freight #42202) Computer with design software such as CorelDraw (Corel Corporation) or Illustrator (Adobe Systems Incorporated) Cover glass (Corning No. 1, 24×60 mm #2955-246) Dremel® rotary tool with a diamond coated bit (Dremel #7134) Epoxy, 5 minute (Devcon #14210) Gastight syringes 1000 μl (×4) and 50 μl (×1) (Hamilton #1001 and # 1705) Glass Microscope slides (Fisher Scientific 25×75×1 mm #12-550-A3) Heat block or water bath for maintaining temperature for various reactions High-pressure mercury plasma arc-discharge lamp for curing the optical adhesive (Zeiss 100 watt HBO lamp).

    Concentration Assay:

    Article Title: Fidelity Index Determination of DNA Methyltransferases
    Article Snippet: .. A standard methylation assay consisted of a 30 µL reaction containing a fixed concentration of [Methyl-3 H]-S -Adenosylmethionine (SAM, Perkin Elmer #NET155V001MC), 1 µg of N6 -methyladenine free λ DNA (NEB #N3013), and varying concentrations of methyltransferase from the two-fold dilution series (making up 10% of the final volume) in the appropriate methyltransferase buffer (see below). ..

    CpG Methylation Assay:

    Article Title: Multidimensional assessment of alveolar T cells in critically ill patients
    Article Snippet: .. Bisulfite conversion efficiency averaged 99.2% (SD 0.098%), which was calculated by the global CpG methylation of unmethylated λ-bacteriophage DNA (New England BioLabs N3013S) spiked in at a 1:200 mass ratio to each sample. .. Random priming and adapter ligation were performed with the Pico Methyl-Seq Library Prep Kit (Zymo Research) using Illumina TruSeq indices.

    Article Title: Uncoupling histone H3K4 trimethylation from developmental gene expression via an equilibrium of COMPASS, Polycomb and DNA methylation.
    Article Snippet: .. The COMPASS protein family catalyzes histone H3 Lys 4 (H3K4) methylation and its members are essential for regulating gene expression. .. The COMPASS protein family catalyzes histone H3 Lys 4 (H3K4) methylation and its members are essential for regulating gene expression.

    Plasmid Preparation:

    Article Title: Characterization of innate immunity genes in the parasitic nematode Brugia malayi
    Article Snippet: .. Preparation of dsDNA Unmethylated dsDNA was obtained from Lambda vector DNA (Cat. #N3013, New England Biolabs). .. A 3 ml stock of 2.2 mg/ml Lambda DNA was diluted five-fold in RNAse free water and sheared to approximately 150 bp using AFA Covaris microtubes (Cat. #520045, Covaris) on a Covaris S2 sonicator.

    Polymerase Chain Reaction:

    Article Title: Exploring protein-DNA interactions in 3D using in situ construction, manipulation, and visualization of individual DNA-dumbbells with optical traps, microfluidics, and fluorescence microscopy
    Article Snippet: .. 5-(3-aminoallyl) dUTP (Fermentas #R0091) Adenosine 5′-O-(3-thiotriphosphate) (ATPγS Calbiotech #119120) ATTO565 NHS-ester (ATTO-TEC GmbH #AD 565-31) Avidin agarose (Thermo Scientific #20219) Biotin-11-dGTP (PerkinElmer #NEL541) Bovine serum albumin (BSA, Sigma-Aldrich #A-9647) Deoxyribonucleoside triphosphates (dNTPs: Invitrogen dATP (#55082), TTP (#55085), dCTP (#55083) and dGTP (#55084) for PCR) Dithiothreitol (DTT Fisher Scientific #BP172-25) CAUTION causes eye and skin irritation; handle wearing goggles, lab coat and gloves Ethanol (EtOH Gold Shield Chemical Company #43196-117) CAUTION flammable; avoid open flames Ethylenediaminetetraacetic acid (EDTA, J. T. Baker #8993-01) Klenow Fragment DNA polymerase I (NEB #M0212S) Magnesium acetate (Mg(OAc)2 J. T. Baker #2424-01) Magnesium chloride (MgCl2 ) Methanol (MeOH Fisher Scientific #A412-4) CAUTION flammable; avoid open flames NEB buffer #2 (NEB buffer supplied with Klenow Fragment DNA polymerase I NEB #B7002S) Phage λ DNA (NEB #N3013S) Potassium hydroxide (KOH Sigma-Aldrich #221473-1KG) CAUTION corrosive; handle wearing goggles, lab coat and gloves Primers for polymerase chain reaction (PCR) to yield a 430 bp product identical to λ DNA between base pairs 23,788 and 24,217: forward primer 5′-biotin-ACTGTTCTTGCGGTTTGGAGG-3′ reverse primer 5′-CTATCGGAAGTTCACCAGCCAG-3′ (can be purchased from any high quality source such as Sigma, Integrated DNA Technologies, or Invitrogen) Sodium bicarbonate (NaHCO3 J. T. Baker #3506-05) Sodium chloride (NaCl) Sodium hydroxide (NaOH) Streptavidin-coated polystyrene beads, 1 μm (Bangs Laboratories #CP01N/10021) Sucrose (Sigma-Aldrich #S7903-5KG) ThermoPol buffer (NEB buffer supplied with VentR ® (exo− ) DNA polymerase NEB #B9004S) Tris acetate (TrisOAc TRIZMA base Sigma-Aldrich #T-1503 + Acetic acid EMD #AX0073-9) CAUTION Acetic acid is corrosive; handle wearing goggles, lab coat and gloves in ventilated hood Water, ultrapure Type 1 (Nanopure (Barnstead) or Milli-Q (Millipore)) VentR ® (exo− ) DNA Polymerase (NEB #M0257) YOYO-1 (Invitrogen #Y3601) .. Abrasive blasting cabinet (Harbor Freight #42202) Computer with design software such as CorelDraw (Corel Corporation) or Illustrator (Adobe Systems Incorporated) Cover glass (Corning No. 1, 24×60 mm #2955-246) Dremel® rotary tool with a diamond coated bit (Dremel #7134) Epoxy, 5 minute (Devcon #14210) Gastight syringes 1000 μl (×4) and 50 μl (×1) (Hamilton #1001 and # 1705) Glass Microscope slides (Fisher Scientific 25×75×1 mm #12-550-A3) Heat block or water bath for maintaining temperature for various reactions High-pressure mercury plasma arc-discharge lamp for curing the optical adhesive (Zeiss 100 watt HBO lamp).

    Hood:

    Article Title: Exploring protein-DNA interactions in 3D using in situ construction, manipulation, and visualization of individual DNA-dumbbells with optical traps, microfluidics, and fluorescence microscopy
    Article Snippet: .. 5-(3-aminoallyl) dUTP (Fermentas #R0091) Adenosine 5′-O-(3-thiotriphosphate) (ATPγS Calbiotech #119120) ATTO565 NHS-ester (ATTO-TEC GmbH #AD 565-31) Avidin agarose (Thermo Scientific #20219) Biotin-11-dGTP (PerkinElmer #NEL541) Bovine serum albumin (BSA, Sigma-Aldrich #A-9647) Deoxyribonucleoside triphosphates (dNTPs: Invitrogen dATP (#55082), TTP (#55085), dCTP (#55083) and dGTP (#55084) for PCR) Dithiothreitol (DTT Fisher Scientific #BP172-25) CAUTION causes eye and skin irritation; handle wearing goggles, lab coat and gloves Ethanol (EtOH Gold Shield Chemical Company #43196-117) CAUTION flammable; avoid open flames Ethylenediaminetetraacetic acid (EDTA, J. T. Baker #8993-01) Klenow Fragment DNA polymerase I (NEB #M0212S) Magnesium acetate (Mg(OAc)2 J. T. Baker #2424-01) Magnesium chloride (MgCl2 ) Methanol (MeOH Fisher Scientific #A412-4) CAUTION flammable; avoid open flames NEB buffer #2 (NEB buffer supplied with Klenow Fragment DNA polymerase I NEB #B7002S) Phage λ DNA (NEB #N3013S) Potassium hydroxide (KOH Sigma-Aldrich #221473-1KG) CAUTION corrosive; handle wearing goggles, lab coat and gloves Primers for polymerase chain reaction (PCR) to yield a 430 bp product identical to λ DNA between base pairs 23,788 and 24,217: forward primer 5′-biotin-ACTGTTCTTGCGGTTTGGAGG-3′ reverse primer 5′-CTATCGGAAGTTCACCAGCCAG-3′ (can be purchased from any high quality source such as Sigma, Integrated DNA Technologies, or Invitrogen) Sodium bicarbonate (NaHCO3 J. T. Baker #3506-05) Sodium chloride (NaCl) Sodium hydroxide (NaOH) Streptavidin-coated polystyrene beads, 1 μm (Bangs Laboratories #CP01N/10021) Sucrose (Sigma-Aldrich #S7903-5KG) ThermoPol buffer (NEB buffer supplied with VentR ® (exo− ) DNA polymerase NEB #B9004S) Tris acetate (TrisOAc TRIZMA base Sigma-Aldrich #T-1503 + Acetic acid EMD #AX0073-9) CAUTION Acetic acid is corrosive; handle wearing goggles, lab coat and gloves in ventilated hood Water, ultrapure Type 1 (Nanopure (Barnstead) or Milli-Q (Millipore)) VentR ® (exo− ) DNA Polymerase (NEB #M0257) YOYO-1 (Invitrogen #Y3601) .. Abrasive blasting cabinet (Harbor Freight #42202) Computer with design software such as CorelDraw (Corel Corporation) or Illustrator (Adobe Systems Incorporated) Cover glass (Corning No. 1, 24×60 mm #2955-246) Dremel® rotary tool with a diamond coated bit (Dremel #7134) Epoxy, 5 minute (Devcon #14210) Gastight syringes 1000 μl (×4) and 50 μl (×1) (Hamilton #1001 and # 1705) Glass Microscope slides (Fisher Scientific 25×75×1 mm #12-550-A3) Heat block or water bath for maintaining temperature for various reactions High-pressure mercury plasma arc-discharge lamp for curing the optical adhesive (Zeiss 100 watt HBO lamp).

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  • Team
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  • Contact
  • Bioz Stars
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  • 97
    New England Biolabs n 6 methyladenine free bacteriophage lambda
    N 6 Methyladenine Free Bacteriophage Lambda, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n 6 methyladenine free bacteriophage lambda/product/New England Biolabs
    Average 97 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    n 6 methyladenine free bacteriophage lambda - by Bioz Stars, 2020-09
    97/100 stars
      Buy from Supplier

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