n3011  (New England Biolabs)


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  • 99
    Name:
    Lambda DNA
    Description:
    Lambda DNA 1 250 ug
    Catalog Number:
    n3011l
    Price:
    276
    Size:
    1 250 ug
    Category:
    Genomic DNA
    Buy from Supplier


    Structured Review

    New England Biolabs n3011
    Lambda DNA
    Lambda DNA 1 250 ug
    https://www.bioz.com/result/n3011/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    n3011 - by Bioz Stars, 2020-08
    99/100 stars

    Related Products / Commonly Used Together

    λ dna
    t4 polynucleotide kinase

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    Related Articles

    In Vitro:

    Article Title: High-Throughput Analysis of Global DNA Methylation Using Methyl-Sensitive Digestion
    Article Snippet: .. In vitro methylation of lambda DNA Fully methylated bacteriophage lambda (λ) DNA was acquired by incubating 1 μg of λ DNA in a 20-μl reaction containing 1 U of M.SssI methylase, 1X NEBuffer 2, and freshly-prepared 160 μM S-adenosylmethionine at 37°C for 1 h, followed by heat inactivation of the enzyme at 65°C for 20 min ( ). ..

    Methylation:

    Article Title: High-Throughput Analysis of Global DNA Methylation Using Methyl-Sensitive Digestion
    Article Snippet: .. In vitro methylation of lambda DNA Fully methylated bacteriophage lambda (λ) DNA was acquired by incubating 1 μg of λ DNA in a 20-μl reaction containing 1 U of M.SssI methylase, 1X NEBuffer 2, and freshly-prepared 160 μM S-adenosylmethionine at 37°C for 1 h, followed by heat inactivation of the enzyme at 65°C for 20 min ( ). ..

    Lambda DNA Preparation:

    Article Title: Single Molecule Hydrodynamic Separation Allows Sensitive and Quantitative Analysis of DNA Conformation and Binding Interactions in Free Solution
    Article Snippet: .. Lambda DNA, HindIII digested Lambda DNA, 1 kb DNA Ladder, and Supercoiled DNA Ladder (all from New England Biolabs, Inc.) were used as double stranded DNA samples. .. Staining was performed at 5 or 10 ng/μL total dsDNA concentration and 1 μM TOTO-3 Iodide (Life Technologies) for at least 1 hour in the dark.

    Article Title: High-Throughput Analysis of Global DNA Methylation Using Methyl-Sensitive Digestion
    Article Snippet: .. In vitro methylation of lambda DNA Fully methylated bacteriophage lambda (λ) DNA was acquired by incubating 1 μg of λ DNA in a 20-μl reaction containing 1 U of M.SssI methylase, 1X NEBuffer 2, and freshly-prepared 160 μM S-adenosylmethionine at 37°C for 1 h, followed by heat inactivation of the enzyme at 65°C for 20 min ( ). ..

    Purification:

    Article Title: Efficient modification of λ-DNA substrates for single-molecule studies
    Article Snippet: .. Inserting synthetic oligonucleotides into λ-DNA Recombinant λ-DNA was obtained from strain IF189, which was modified and purified as described above, and 25 μg of the DNA was incubated with 150 U of Nt.BspQI (NEB# R0644S) in a 250 µL reaction with 1X buffer 3.1 (NEB #B7203) at 55 °C for 1 hour. .. The reaction was halted with 1 U of proteinase K (NEB #P8107S) for 1 hour at 55 °C.

    Incubation:

    Article Title: Efficient modification of λ-DNA substrates for single-molecule studies
    Article Snippet: .. Inserting synthetic oligonucleotides into λ-DNA Recombinant λ-DNA was obtained from strain IF189, which was modified and purified as described above, and 25 μg of the DNA was incubated with 150 U of Nt.BspQI (NEB# R0644S) in a 250 µL reaction with 1X buffer 3.1 (NEB #B7203) at 55 °C for 1 hour. .. The reaction was halted with 1 U of proteinase K (NEB #P8107S) for 1 hour at 55 °C.

    Modification:

    Article Title: Efficient modification of λ-DNA substrates for single-molecule studies
    Article Snippet: .. Inserting synthetic oligonucleotides into λ-DNA Recombinant λ-DNA was obtained from strain IF189, which was modified and purified as described above, and 25 μg of the DNA was incubated with 150 U of Nt.BspQI (NEB# R0644S) in a 250 µL reaction with 1X buffer 3.1 (NEB #B7203) at 55 °C for 1 hour. .. The reaction was halted with 1 U of proteinase K (NEB #P8107S) for 1 hour at 55 °C.

    Recombinant:

    Article Title: Efficient modification of λ-DNA substrates for single-molecule studies
    Article Snippet: .. Inserting synthetic oligonucleotides into λ-DNA Recombinant λ-DNA was obtained from strain IF189, which was modified and purified as described above, and 25 μg of the DNA was incubated with 150 U of Nt.BspQI (NEB# R0644S) in a 250 µL reaction with 1X buffer 3.1 (NEB #B7203) at 55 °C for 1 hour. .. The reaction was halted with 1 U of proteinase K (NEB #P8107S) for 1 hour at 55 °C.

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    New England Biolabs hindiii digested lambda dna
    The effects of both sodium chloride (blue) and magnesium chloride (red) on the packing density of double stranded and single stranded <t>DNA</t> is probed by comparing their relative mobilities in the same 1.6 μm diameter capillary. (a) <t>HindIII</t> digested
    Hindiii Digested Lambda Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hindiii digested lambda dna/product/New England Biolabs
    Average 99 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    hindiii digested lambda dna - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    The effects of both sodium chloride (blue) and magnesium chloride (red) on the packing density of double stranded and single stranded DNA is probed by comparing their relative mobilities in the same 1.6 μm diameter capillary. (a) HindIII digested

    Journal: Journal of the American Chemical Society

    Article Title: Single Molecule Hydrodynamic Separation Allows Sensitive and Quantitative Analysis of DNA Conformation and Binding Interactions in Free Solution

    doi: 10.1021/jacs.5b10983

    Figure Lengend Snippet: The effects of both sodium chloride (blue) and magnesium chloride (red) on the packing density of double stranded and single stranded DNA is probed by comparing their relative mobilities in the same 1.6 μm diameter capillary. (a) HindIII digested

    Article Snippet: Lambda DNA, HindIII digested Lambda DNA, 1 kb DNA Ladder, and Supercoiled DNA Ladder (all from New England Biolabs, Inc.) were used as double stranded DNA samples.

    Techniques:

    T4 DNA relaxation within nanoslits after electrokinetic loading. Plots show the relaxation kinetics (0.4 mM ionic strength, New England Biolabs buffer 4) (see Materials and Methods ) of two DNA molecules [the first (black) enters nanoslits in a folded

    Journal:

    Article Title: A single-molecule barcoding system using nanoslits for DNA analysis

    doi: 10.1073/pnas.0611151104

    Figure Lengend Snippet: T4 DNA relaxation within nanoslits after electrokinetic loading. Plots show the relaxation kinetics (0.4 mM ionic strength, New England Biolabs buffer 4) (see Materials and Methods ) of two DNA molecules [the first (black) enters nanoslits in a folded

    Article Snippet: DNA samples [1 ng/μl λ DNA (New England Biolabs, Ipswich, MA), 0.78 ng/μl T4 DNA (Waco Chemicals, Waco, TX), and E. coli DNA ( )], contained 0.25 μM YOYO-1, from 1× to 0.01× TE buffer, 4% (vol/vol) 2-mercaptoethanol, and 0.1% (wt/vol) POP6 (Applied Biosystems, Foster City, CA) to suppress electroendosmosis ( ).

    Techniques:

    DNA stretch varies with TE concentration. ( A ) Ionic strength varied through successive 5-, 10-, 15-, 20-, 50-, and 100-fold dilutions of 1× TE buffer (ionic strength, 8.4 mM). The reciprocal TE buffer concentrations vs. the stretch of λ

    Journal:

    Article Title: A single-molecule barcoding system using nanoslits for DNA analysis

    doi: 10.1073/pnas.0611151104

    Figure Lengend Snippet: DNA stretch varies with TE concentration. ( A ) Ionic strength varied through successive 5-, 10-, 15-, 20-, 50-, and 100-fold dilutions of 1× TE buffer (ionic strength, 8.4 mM). The reciprocal TE buffer concentrations vs. the stretch of λ

    Article Snippet: DNA samples [1 ng/μl λ DNA (New England Biolabs, Ipswich, MA), 0.78 ng/μl T4 DNA (Waco Chemicals, Waco, TX), and E. coli DNA ( )], contained 0.25 μM YOYO-1, from 1× to 0.01× TE buffer, 4% (vol/vol) 2-mercaptoethanol, and 0.1% (wt/vol) POP6 (Applied Biosystems, Foster City, CA) to suppress electroendosmosis ( ).

    Techniques: Concentration Assay

    Effective persistence lengths of C 8 -B Sso7d -coated λ-DNA versus the mole ratio [C 8 -B Sso7d ]/[DNA(bp)] of protein to DNA basepairs, as deduced by fitting optical tweezer force extension curves with the wormlike chain model ( eq 5 ). Solution conditions: 10 mM Tris-HCl, pH 7.4, DNA concentration C DNA = 1.58 μg/mL. Note that the upper x -axis shows the protein concentration in nM. Dashed line is a guide to the eye.

    Journal: Macromolecules

    Article Title: Force and Scale Dependence of the Elasticity of Self-Assembled DNA Bottle Brushes

    doi: 10.1021/acs.macromol.7b01795

    Figure Lengend Snippet: Effective persistence lengths of C 8 -B Sso7d -coated λ-DNA versus the mole ratio [C 8 -B Sso7d ]/[DNA(bp)] of protein to DNA basepairs, as deduced by fitting optical tweezer force extension curves with the wormlike chain model ( eq 5 ). Solution conditions: 10 mM Tris-HCl, pH 7.4, DNA concentration C DNA = 1.58 μg/mL. Note that the upper x -axis shows the protein concentration in nM. Dashed line is a guide to the eye.

    Article Snippet: Optical Tweezer Experiments For the optical tweezer experiment, λ-DNA molecules (New England Biolabs) are end-labeled with biotin attached by one end to a streptavidin-coated bead of 3 μm diameter (Bangs Laboratories) and by the other end to a streptavidin-coated coverslip (Xenopore Corp.).

    Techniques: Concentration Assay, Protein Concentration

    Bottle-brush thickness from small-angle X-ray scattering. Scattering intensity (arbitrary units, a.u.) versus magnitude q of the wavevector in nm –1 . Solution conditions are 10 mM Tris-HCl, pH 7.6. Blue squares are the scattering intensities for 100 μg/mL λ-DNA coated with the C 8 -B Sso7d diblock protein polymer at a protein to DNA ratio of [C 8 -B Sso7d ]/[DNA(bp)] of 0.5 ptn/bp; black circles are the scattering intensities for 30 mg/mL free C 8 -B Sso7d protein polymer. In the figure, the scattering intensity for the free C 8 -B Sso7d protein polymer is scaled to match the high- q scattering of the of C 8 -B Sso7d /DNA bottle-brush complex. The red line is a fit to a polymer coil model with excluded volume; the orange line is the sum of a contribution due to excluded volume polymer coils (representing the excess unbound protein polymers) and a contribution due to randomly oriented rigid cylinders. Separate contributions of polymer coils and cylinders are indicated in gray.

    Journal: Macromolecules

    Article Title: Force and Scale Dependence of the Elasticity of Self-Assembled DNA Bottle Brushes

    doi: 10.1021/acs.macromol.7b01795

    Figure Lengend Snippet: Bottle-brush thickness from small-angle X-ray scattering. Scattering intensity (arbitrary units, a.u.) versus magnitude q of the wavevector in nm –1 . Solution conditions are 10 mM Tris-HCl, pH 7.6. Blue squares are the scattering intensities for 100 μg/mL λ-DNA coated with the C 8 -B Sso7d diblock protein polymer at a protein to DNA ratio of [C 8 -B Sso7d ]/[DNA(bp)] of 0.5 ptn/bp; black circles are the scattering intensities for 30 mg/mL free C 8 -B Sso7d protein polymer. In the figure, the scattering intensity for the free C 8 -B Sso7d protein polymer is scaled to match the high- q scattering of the of C 8 -B Sso7d /DNA bottle-brush complex. The red line is a fit to a polymer coil model with excluded volume; the orange line is the sum of a contribution due to excluded volume polymer coils (representing the excess unbound protein polymers) and a contribution due to randomly oriented rigid cylinders. Separate contributions of polymer coils and cylinders are indicated in gray.

    Article Snippet: Optical Tweezer Experiments For the optical tweezer experiment, λ-DNA molecules (New England Biolabs) are end-labeled with biotin attached by one end to a streptavidin-coated bead of 3 μm diameter (Bangs Laboratories) and by the other end to a streptavidin-coated coverslip (Xenopore Corp.).

    Techniques:

    The workflow of the methyl-sensitive fluorescence polarization (MSFP) assay. Lambda (λ) and human genomic DNA are restricted by MspI (white) or by HpaII each alone or by a combination of HpaII and HpyCH4IV (grey) (Step 1). Subsequently, digested DNA with CpG overhangs at the 5' termini are terminally extended with fluorescence-labelled TAMRA-dCTP (C*, *C) (Step 2). TAMRA-dCTP incorporated into DNA is quantified by fluorescence polarization directly on the plate without additional purification procedures.

    Journal: PLoS ONE

    Article Title: High-Throughput Analysis of Global DNA Methylation Using Methyl-Sensitive Digestion

    doi: 10.1371/journal.pone.0163184

    Figure Lengend Snippet: The workflow of the methyl-sensitive fluorescence polarization (MSFP) assay. Lambda (λ) and human genomic DNA are restricted by MspI (white) or by HpaII each alone or by a combination of HpaII and HpyCH4IV (grey) (Step 1). Subsequently, digested DNA with CpG overhangs at the 5' termini are terminally extended with fluorescence-labelled TAMRA-dCTP (C*, *C) (Step 2). TAMRA-dCTP incorporated into DNA is quantified by fluorescence polarization directly on the plate without additional purification procedures.

    Article Snippet: In vitro methylation of lambda DNA Fully methylated bacteriophage lambda (λ) DNA was acquired by incubating 1 μg of λ DNA in a 20-μl reaction containing 1 U of M.SssI methylase, 1X NEBuffer 2, and freshly-prepared 160 μM S-adenosylmethionine at 37°C for 1 h, followed by heat inactivation of the enzyme at 65°C for 20 min ( ).

    Techniques: Fluorescence, Purification