quick load purple 1 kb dna ladder  (New England Biolabs)


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    Name:
    Quick Load Purple 1 kb DNA Ladder
    Description:
    Quick Load Purple 1 kb DNA Ladder 375 gel lanes
    Catalog Number:
    n0552l
    Price:
    129
    Size:
    375 gel lanes
    Category:
    DNA Ladders
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    Structured Review

    New England Biolabs quick load purple 1 kb dna ladder
    Quick Load Purple 1 kb DNA Ladder
    Quick Load Purple 1 kb DNA Ladder 375 gel lanes
    https://www.bioz.com/result/quick load purple 1 kb dna ladder/product/New England Biolabs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quick load purple 1 kb dna ladder - by Bioz Stars, 2020-02
    92/100 stars

    Related Products / Commonly Used Together

    individual plasmid dna
    miniready agarose gel
    1xtbe

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    Related Articles

    Marker:

    Article Title: Single-stranded binding proteins and helicase enhance the activity of prokaryotic argonautes in vitro
    Article Snippet: .. The marker ( M ) is NEB® Quick-Load Purple 2-Log DNA Ladder. (PNG) Click here for additional data file. ..

    Agarose Gel Electrophoresis:

    Article Title: Measuring UV Photoproduct Repair in Isolated Telomeres and Bulk Genomic DNA
    Article Snippet: Paragraph title: 2.3. Agarose Gel Electrophoresis ... Quick-Load Purple 2-Log DNA ladder (New England Biolabs, Ipswich, MA), or an equivalent DNA ladder.

    Article Title: A Simple Procedure for Creating Scalable Phenotypic Screening Assays in Human Neurons
    Article Snippet: .. Individual plasmid DNA were incubated with appropriate restriction enzymes (New England Biolabs, Ipswich, MA) and NE Buffer (cat. no. B7201, NEB) for 1 h. Purple loading dye (cat. no. B7024S, NEB) was added to each sample which were loaded carefully on MiniReady Agarose Gel prepared in 1XTBE (cat. no. 101-3004, Biorad) alongside with Quick load purple 1 kb DNA ladder as a reference standard (cat. no. N0552G, NEB). .. The results demonstrated appropriate digest products for each plasmid as well as the maxi-prep RFLP and can be found in the supplemental data (Figure ).

    Isolation:

    Article Title: A Simple Procedure for Creating Scalable Phenotypic Screening Assays in Human Neurons
    Article Snippet: Plasmid DNA was isolated using mini-prep columns (cat. no. 27104, Qiagen) and purity and yield were determined using Nanodrop 2000 (cat. no. ND-2000). .. Individual plasmid DNA were incubated with appropriate restriction enzymes (New England Biolabs, Ipswich, MA) and NE Buffer (cat. no. B7201, NEB) for 1 h. Purple loading dye (cat. no. B7024S, NEB) was added to each sample which were loaded carefully on MiniReady Agarose Gel prepared in 1XTBE (cat. no. 101-3004, Biorad) alongside with Quick load purple 1 kb DNA ladder as a reference standard (cat. no. N0552G, NEB).

    Construct:

    Article Title: A Simple Procedure for Creating Scalable Phenotypic Screening Assays in Human Neurons
    Article Snippet: Plasmid generation The Ngn2 transcription factor mediated differentiation protocol uses two lentiviral constructs as described previously (i) lentivirus vector (TetO-NGN2-puro) with Addgene plasmid accession ID: 52047, and (ii) lentivirus vector (FUW-M2rtTA) with Addgene plasmid accession ID: 20342 and an optional (iii) lentivirus vector (Tet-O-FUW-EGFP) with Addgene plasmid accession ID: 30130. .. Individual plasmid DNA were incubated with appropriate restriction enzymes (New England Biolabs, Ipswich, MA) and NE Buffer (cat. no. B7201, NEB) for 1 h. Purple loading dye (cat. no. B7024S, NEB) was added to each sample which were loaded carefully on MiniReady Agarose Gel prepared in 1XTBE (cat. no. 101-3004, Biorad) alongside with Quick load purple 1 kb DNA ladder as a reference standard (cat. no. N0552G, NEB).

    SYBR Green Assay:

    Article Title: Measuring UV Photoproduct Repair in Isolated Telomeres and Bulk Genomic DNA
    Article Snippet: Quick-Load Purple 2-Log DNA ladder (New England Biolabs, Ipswich, MA), or an equivalent DNA ladder. .. Quick-Load Purple 2-Log DNA ladder (New England Biolabs, Ipswich, MA), or an equivalent DNA ladder.

    Concentration Assay:

    Article Title: Single-stranded binding proteins and helicase enhance the activity of prokaryotic argonautes in vitro
    Article Snippet: The marker ( M ) is NEB® Quick-Load Purple 2-Log DNA Ladder. (PNG) Click here for additional data file. .. Chloride concentration was carefully controlled by using a reaction buffer consisting of 20 mM Bis-tris propane, adjusted to pH 8.8 with acetic acid, 2 mM magnesium sulfate, and varying amounts of sodium chloride spiked in.

    Incubation:

    Article Title: A Simple Procedure for Creating Scalable Phenotypic Screening Assays in Human Neurons
    Article Snippet: .. Individual plasmid DNA were incubated with appropriate restriction enzymes (New England Biolabs, Ipswich, MA) and NE Buffer (cat. no. B7201, NEB) for 1 h. Purple loading dye (cat. no. B7024S, NEB) was added to each sample which were loaded carefully on MiniReady Agarose Gel prepared in 1XTBE (cat. no. 101-3004, Biorad) alongside with Quick load purple 1 kb DNA ladder as a reference standard (cat. no. N0552G, NEB). .. The results demonstrated appropriate digest products for each plasmid as well as the maxi-prep RFLP and can be found in the supplemental data (Figure ).

    Activity Assay:

    Article Title: Single-stranded binding proteins and helicase enhance the activity of prokaryotic argonautes in vitro
    Article Snippet: Paragraph title: Supporting information Tt Ago cuts supercoiled plasmid. Effect of pH on Tt Ago activity. Effect of salt and temperature on Tt Ago activity. Effect of salt on Tt Ago activity in the presence of ET SSB. Tt Ago with UvrD-like helicases from various other thermophilic bacterial and archaeal organisms. Non-specific activity of Pf Ago on double-stranded DNA substrates is increased in the presence of ET SSB. SSB binding activity. Individual point mutations of the Tt Ago catalytic tetrad eliminate activity. Sequence and GC content information for guides. Sequence and GC content information for CE substrates. Methods and discussion pertaining to Ng Ago and ET SSB. ... The marker ( M ) is NEB® Quick-Load Purple 2-Log DNA Ladder. (PNG) Click here for additional data file.

    Sequencing:

    Article Title: Single-stranded binding proteins and helicase enhance the activity of prokaryotic argonautes in vitro
    Article Snippet: Paragraph title: Supporting information Tt Ago cuts supercoiled plasmid. Effect of pH on Tt Ago activity. Effect of salt and temperature on Tt Ago activity. Effect of salt on Tt Ago activity in the presence of ET SSB. Tt Ago with UvrD-like helicases from various other thermophilic bacterial and archaeal organisms. Non-specific activity of Pf Ago on double-stranded DNA substrates is increased in the presence of ET SSB. SSB binding activity. Individual point mutations of the Tt Ago catalytic tetrad eliminate activity. Sequence and GC content information for guides. Sequence and GC content information for CE substrates. Methods and discussion pertaining to Ng Ago and ET SSB. ... The marker ( M ) is NEB® Quick-Load Purple 2-Log DNA Ladder. (PNG) Click here for additional data file.

    Binding Assay:

    Article Title: Single-stranded binding proteins and helicase enhance the activity of prokaryotic argonautes in vitro
    Article Snippet: Paragraph title: Supporting information Tt Ago cuts supercoiled plasmid. Effect of pH on Tt Ago activity. Effect of salt and temperature on Tt Ago activity. Effect of salt on Tt Ago activity in the presence of ET SSB. Tt Ago with UvrD-like helicases from various other thermophilic bacterial and archaeal organisms. Non-specific activity of Pf Ago on double-stranded DNA substrates is increased in the presence of ET SSB. SSB binding activity. Individual point mutations of the Tt Ago catalytic tetrad eliminate activity. Sequence and GC content information for guides. Sequence and GC content information for CE substrates. Methods and discussion pertaining to Ng Ago and ET SSB. ... The marker ( M ) is NEB® Quick-Load Purple 2-Log DNA Ladder. (PNG) Click here for additional data file.

    Plasmid Preparation:

    Article Title: A Simple Procedure for Creating Scalable Phenotypic Screening Assays in Human Neurons
    Article Snippet: .. Individual plasmid DNA were incubated with appropriate restriction enzymes (New England Biolabs, Ipswich, MA) and NE Buffer (cat. no. B7201, NEB) for 1 h. Purple loading dye (cat. no. B7024S, NEB) was added to each sample which were loaded carefully on MiniReady Agarose Gel prepared in 1XTBE (cat. no. 101-3004, Biorad) alongside with Quick load purple 1 kb DNA ladder as a reference standard (cat. no. N0552G, NEB). .. The results demonstrated appropriate digest products for each plasmid as well as the maxi-prep RFLP and can be found in the supplemental data (Figure ).

    Article Title: Single-stranded binding proteins and helicase enhance the activity of prokaryotic argonautes in vitro
    Article Snippet: Paragraph title: Supporting information Tt Ago cuts supercoiled plasmid. Effect of pH on Tt Ago activity. Effect of salt and temperature on Tt Ago activity. Effect of salt on Tt Ago activity in the presence of ET SSB. Tt Ago with UvrD-like helicases from various other thermophilic bacterial and archaeal organisms. Non-specific activity of Pf Ago on double-stranded DNA substrates is increased in the presence of ET SSB. SSB binding activity. Individual point mutations of the Tt Ago catalytic tetrad eliminate activity. Sequence and GC content information for guides. Sequence and GC content information for CE substrates. Methods and discussion pertaining to Ng Ago and ET SSB. ... The marker ( M ) is NEB® Quick-Load Purple 2-Log DNA Ladder. (PNG) Click here for additional data file.

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  • Team
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  • 92
    New England Biolabs quick load purple 1 kb dna ladder
    Quick Load Purple 1 Kb Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quick load purple 1 kb dna ladder/product/New England Biolabs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quick load purple 1 kb dna ladder - by Bioz Stars, 2020-02
    92/100 stars
      Buy from Supplier

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