neb quick load purple  (New England Biolabs)


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    Name:
    Quick Load Purple 1 kb Plus DNA Ladder
    Description:
    Quick Load Purple 2 log DNA Ladder 0 1 10 0 kb 750 gel lanes
    Catalog Number:
    N0550L
    Price:
    184
    Category:
    DNA Ladders
    Size:
    750 gel lanes
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    New England Biolabs neb quick load purple
    Quick Load Purple 1 kb Plus DNA Ladder
    Quick Load Purple 2 log DNA Ladder 0 1 10 0 kb 750 gel lanes
    https://www.bioz.com/result/neb quick load purple/product/New England Biolabs
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    neb quick load purple - by Bioz Stars, 2021-05
    97/100 stars

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    1) Product Images from "Genome sequence and plasmid transformation of the model high-yield bacterial cellulose producer Gluconacetobacter hansenii ATCC 53582"

    Article Title: Genome sequence and plasmid transformation of the model high-yield bacterial cellulose producer Gluconacetobacter hansenii ATCC 53582

    Journal: Scientific Reports

    doi: 10.1038/srep23635

    Colony PCR of G. hansenii ATCC 53582 transformed with pSEVA331Bb, pSEVA351, pBla-Vhb-122 and pBAV1K. 1, 2 and 3 – biological replicates of transformed ATCC 53582 used for colony PCR; Pos – Positive control (PCR with pure plasmid DNA), Neg – negative control (colony PCR of untransformed ATCC 53582); L – NEB Quick-Load Purple 2-log DNA ladder. ATCC 53582 were transformed with pSEVA311, pSEVA321, pSEVA331Bb, pSEVA341, pSEVA351, pBla-Vhb-122, pBAV1K, pSB1C3 and pBca1020. Of the used plasmids, colonies were present with pSEVA331Bb, pSEVA351, pBla-Vhb-122 and pBAV1K, and transformants were subsequently confirmed with colony PCR. Although failure to obtain colonies with other plasmids may be due to low transformation efficiencies, we were unable to obtain any transformants with these plasmids in repeat experiments, indicating incompatible origins of replication.
    Figure Legend Snippet: Colony PCR of G. hansenii ATCC 53582 transformed with pSEVA331Bb, pSEVA351, pBla-Vhb-122 and pBAV1K. 1, 2 and 3 – biological replicates of transformed ATCC 53582 used for colony PCR; Pos – Positive control (PCR with pure plasmid DNA), Neg – negative control (colony PCR of untransformed ATCC 53582); L – NEB Quick-Load Purple 2-log DNA ladder. ATCC 53582 were transformed with pSEVA311, pSEVA321, pSEVA331Bb, pSEVA341, pSEVA351, pBla-Vhb-122, pBAV1K, pSB1C3 and pBca1020. Of the used plasmids, colonies were present with pSEVA331Bb, pSEVA351, pBla-Vhb-122 and pBAV1K, and transformants were subsequently confirmed with colony PCR. Although failure to obtain colonies with other plasmids may be due to low transformation efficiencies, we were unable to obtain any transformants with these plasmids in repeat experiments, indicating incompatible origins of replication.

    Techniques Used: Polymerase Chain Reaction, Transformation Assay, Positive Control, Plasmid Preparation, Negative Control

    Related Articles

    Transfection:

    Article Title: EMMA assembly explained: A step-by-step guide to assemble synthetic mammalian vectors.
    Article Snippet: Construction of expression vectors is imperative for many areas of biological research and the biotechnology industry.. Modular cloning systems for expression vector construction offer a labor- and cost-effective alternative to overcome drawbacks associated with traditional cloning methods. .. Construction of expression vectors is imperative for many areas of biological research and the biotechnology industry.. Modular cloning systems for expression vector construction offer a labor- and cost-effective alternative to overcome drawbacks associated with traditional cloning methods. .. Construction of expression vectors is imperative for many areas of biological research and the biotechnology industry.. Modular cloning systems for expression vector construction offer a labor- and cost-effective alternative to overcome drawbacks associated with traditional cloning methods.

    Plasmid Preparation:

    Article Title: EMMA assembly explained: A step-by-step guide to assemble synthetic mammalian vectors.
    Article Snippet: Construction of expression vectors is imperative for many areas of biological research and the biotechnology industry.. Modular cloning systems for expression vector construction offer a labor- and cost-effective alternative to overcome drawbacks associated with traditional cloning methods. .. Construction of expression vectors is imperative for many areas of biological research and the biotechnology industry.. Modular cloning systems for expression vector construction offer a labor- and cost-effective alternative to overcome drawbacks associated with traditional cloning methods. .. Construction of expression vectors is imperative for many areas of biological research and the biotechnology industry.. Modular cloning systems for expression vector construction offer a labor- and cost-effective alternative to overcome drawbacks associated with traditional cloning methods.

    Purification:

    Article Title: EMMA assembly explained: A step-by-step guide to assemble synthetic mammalian vectors.
    Article Snippet: Construction of expression vectors is imperative for many areas of biological research and the biotechnology industry.. Modular cloning systems for expression vector construction offer a labor- and cost-effective alternative to overcome drawbacks associated with traditional cloning methods. .. Construction of expression vectors is imperative for many areas of biological research and the biotechnology industry.. Modular cloning systems for expression vector construction offer a labor- and cost-effective alternative to overcome drawbacks associated with traditional cloning methods. .. Construction of expression vectors is imperative for many areas of biological research and the biotechnology industry.. Modular cloning systems for expression vector construction offer a labor- and cost-effective alternative to overcome drawbacks associated with traditional cloning methods.

    Staining:

    Article Title: EMMA assembly explained: A step-by-step guide to assemble synthetic mammalian vectors.
    Article Snippet: Construction of expression vectors is imperative for many areas of biological research and the biotechnology industry.. Modular cloning systems for expression vector construction offer a labor- and cost-effective alternative to overcome drawbacks associated with traditional cloning methods. .. Construction of expression vectors is imperative for many areas of biological research and the biotechnology industry.. Modular cloning systems for expression vector construction offer a labor- and cost-effective alternative to overcome drawbacks associated with traditional cloning methods. .. Construction of expression vectors is imperative for many areas of biological research and the biotechnology industry.. Modular cloning systems for expression vector construction offer a labor- and cost-effective alternative to overcome drawbacks associated with traditional cloning methods.

    Sonication:

    Article Title: Chromatin Hyperacetylation Impacts Chromosome Folding by Forming a Nuclear Subcompartment
    Article Snippet: The chromatin was then aliquoted into 1.5 mL low-bind tubes to obtain a concentration corresponding to an initial cell density of 5 × 106 cells per tube for H3K27ac ChIP or 10 ×106 cells per tube for RNA polymerase II ChIP. .. 20 ng of each control (unsonicated and sonicated) was run on a 1X TBE 1% analytical agarose gel with Quick-Load 2-log DNA Ladder (NEB #N0550) to verify successful DNA shearing. .. 50 μl of Protein A/G PLUS-Agarose beads (Santa Cruz Biotechnology #sc-2003) were washed twice with 1 mL of buffer III + 1% Triton ×−100.

    Article Title: Chromatin Hyperacetylation Impacts Chromosome Folding by Forming a Nuclear Subcompartment
    Article Snippet: Benzonase was inactivated by adding 0.5 M EDTA pH 8.0 to a final concentration of 5 mM, and a 10 μL aliquot was removed as the Benzonase-treated control. .. 20 ng of each control (unsonicated, sonicated, and Benzonase-treated) was run on a 1× TBE 1% analytical agarose gel with Quick-Load 2-log DNA Ladder (NEB #N0550) to verify successful DNA shearing. .. The day before immunoprecipitation, 50 μL of DynaBeads Protein A (Thermo Scientific #10001D) were blocked overnight at 4° C with 1 mL o f buffer III (without protease inhibitors) + 1% Triton ×−100, 0.1% SDS, 1 mg/mL BSA.

    Agarose Gel Electrophoresis:

    Article Title: Chromatin Hyperacetylation Impacts Chromosome Folding by Forming a Nuclear Subcompartment
    Article Snippet: The chromatin was then aliquoted into 1.5 mL low-bind tubes to obtain a concentration corresponding to an initial cell density of 5 × 106 cells per tube for H3K27ac ChIP or 10 ×106 cells per tube for RNA polymerase II ChIP. .. 20 ng of each control (unsonicated and sonicated) was run on a 1X TBE 1% analytical agarose gel with Quick-Load 2-log DNA Ladder (NEB #N0550) to verify successful DNA shearing. .. 50 μl of Protein A/G PLUS-Agarose beads (Santa Cruz Biotechnology #sc-2003) were washed twice with 1 mL of buffer III + 1% Triton ×−100.

    Article Title: Chromatin Hyperacetylation Impacts Chromosome Folding by Forming a Nuclear Subcompartment
    Article Snippet: Benzonase was inactivated by adding 0.5 M EDTA pH 8.0 to a final concentration of 5 mM, and a 10 μL aliquot was removed as the Benzonase-treated control. .. 20 ng of each control (unsonicated, sonicated, and Benzonase-treated) was run on a 1× TBE 1% analytical agarose gel with Quick-Load 2-log DNA Ladder (NEB #N0550) to verify successful DNA shearing. .. The day before immunoprecipitation, 50 μL of DynaBeads Protein A (Thermo Scientific #10001D) were blocked overnight at 4° C with 1 mL o f buffer III (without protease inhibitors) + 1% Triton ×−100, 0.1% SDS, 1 mg/mL BSA.

    Transformation Assay:

    Article Title: EMMA assembly explained: A step-by-step guide to assemble synthetic mammalian vectors.
    Article Snippet: .. Construction of expression vectors is imperative for many areas of biological research and the biotechnology industry.. Modular cloning systems for expression vector construction offer a labor- and cost-effective alternative to overcome drawbacks associated with traditional cloning methods. .. Construction of expression vectors is imperative for many areas of biological research and the biotechnology industry.. Modular cloning systems for expression vector construction offer a labor- and cost-effective alternative to overcome drawbacks associated with traditional cloning methods.

    Clone Assay:

    Article Title: EMMA assembly explained: A step-by-step guide to assemble synthetic mammalian vectors.
    Article Snippet: .. Construction of expression vectors is imperative for many areas of biological research and the biotechnology industry.. Modular cloning systems for expression vector construction offer a labor- and cost-effective alternative to overcome drawbacks associated with traditional cloning methods. .. Construction of expression vectors is imperative for many areas of biological research and the biotechnology industry.. Modular cloning systems for expression vector construction offer a labor- and cost-effective alternative to overcome drawbacks associated with traditional cloning methods.

    Polymerase Chain Reaction:

    Article Title: EMMA assembly explained: A step-by-step guide to assemble synthetic mammalian vectors.
    Article Snippet: Construction of expression vectors is imperative for many areas of biological research and the biotechnology industry.. Modular cloning systems for expression vector construction offer a labor- and cost-effective alternative to overcome drawbacks associated with traditional cloning methods. .. Construction of expression vectors is imperative for many areas of biological research and the biotechnology industry.. Modular cloning systems for expression vector construction offer a labor- and cost-effective alternative to overcome drawbacks associated with traditional cloning methods. .. Construction of expression vectors is imperative for many areas of biological research and the biotechnology industry.. Modular cloning systems for expression vector construction offer a labor- and cost-effective alternative to overcome drawbacks associated with traditional cloning methods.

    Gel Extraction:

    Article Title: EMMA assembly explained: A step-by-step guide to assemble synthetic mammalian vectors.
    Article Snippet: Construction of expression vectors is imperative for many areas of biological research and the biotechnology industry.. Modular cloning systems for expression vector construction offer a labor- and cost-effective alternative to overcome drawbacks associated with traditional cloning methods. .. Construction of expression vectors is imperative for many areas of biological research and the biotechnology industry.. Modular cloning systems for expression vector construction offer a labor- and cost-effective alternative to overcome drawbacks associated with traditional cloning methods. .. Construction of expression vectors is imperative for many areas of biological research and the biotechnology industry.. Modular cloning systems for expression vector construction offer a labor- and cost-effective alternative to overcome drawbacks associated with traditional cloning methods.

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    New England Biolabs neb quick load purple
    Colony PCR of G. hansenii ATCC 53582 transformed with pSEVA331Bb, pSEVA351, pBla-Vhb-122 and pBAV1K. 1, 2 and 3 – biological replicates of transformed ATCC 53582 used for colony PCR; Pos – Positive control (PCR with pure plasmid <t>DNA),</t> Neg – negative control (colony PCR of untransformed ATCC 53582); L – <t>NEB</t> Quick-Load Purple 2-log DNA ladder. ATCC 53582 were transformed with pSEVA311, pSEVA321, pSEVA331Bb, pSEVA341, pSEVA351, pBla-Vhb-122, pBAV1K, pSB1C3 and pBca1020. Of the used plasmids, colonies were present with pSEVA331Bb, pSEVA351, pBla-Vhb-122 and pBAV1K, and transformants were subsequently confirmed with colony PCR. Although failure to obtain colonies with other plasmids may be due to low transformation efficiencies, we were unable to obtain any transformants with these plasmids in repeat experiments, indicating incompatible origins of replication.
    Neb Quick Load Purple, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neb quick load purple/product/New England Biolabs
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    neb quick load purple - by Bioz Stars, 2021-05
    97/100 stars
      Buy from Supplier

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    Colony PCR of G. hansenii ATCC 53582 transformed with pSEVA331Bb, pSEVA351, pBla-Vhb-122 and pBAV1K. 1, 2 and 3 – biological replicates of transformed ATCC 53582 used for colony PCR; Pos – Positive control (PCR with pure plasmid DNA), Neg – negative control (colony PCR of untransformed ATCC 53582); L – NEB Quick-Load Purple 2-log DNA ladder. ATCC 53582 were transformed with pSEVA311, pSEVA321, pSEVA331Bb, pSEVA341, pSEVA351, pBla-Vhb-122, pBAV1K, pSB1C3 and pBca1020. Of the used plasmids, colonies were present with pSEVA331Bb, pSEVA351, pBla-Vhb-122 and pBAV1K, and transformants were subsequently confirmed with colony PCR. Although failure to obtain colonies with other plasmids may be due to low transformation efficiencies, we were unable to obtain any transformants with these plasmids in repeat experiments, indicating incompatible origins of replication.

    Journal: Scientific Reports

    Article Title: Genome sequence and plasmid transformation of the model high-yield bacterial cellulose producer Gluconacetobacter hansenii ATCC 53582

    doi: 10.1038/srep23635

    Figure Lengend Snippet: Colony PCR of G. hansenii ATCC 53582 transformed with pSEVA331Bb, pSEVA351, pBla-Vhb-122 and pBAV1K. 1, 2 and 3 – biological replicates of transformed ATCC 53582 used for colony PCR; Pos – Positive control (PCR with pure plasmid DNA), Neg – negative control (colony PCR of untransformed ATCC 53582); L – NEB Quick-Load Purple 2-log DNA ladder. ATCC 53582 were transformed with pSEVA311, pSEVA321, pSEVA331Bb, pSEVA341, pSEVA351, pBla-Vhb-122, pBAV1K, pSB1C3 and pBca1020. Of the used plasmids, colonies were present with pSEVA331Bb, pSEVA351, pBla-Vhb-122 and pBAV1K, and transformants were subsequently confirmed with colony PCR. Although failure to obtain colonies with other plasmids may be due to low transformation efficiencies, we were unable to obtain any transformants with these plasmids in repeat experiments, indicating incompatible origins of replication.

    Article Snippet: The ladder used was NEB Quick-Load Purple 2-log DNA ladder (cat. no. N0550S – NEB) for all tests.

    Techniques: Polymerase Chain Reaction, Transformation Assay, Positive Control, Plasmid Preparation, Negative Control