quick load purple 1 kb plus dna ladder  (New England Biolabs)


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    Name:
    Quick Load Purple 1 kb Plus DNA Ladder
    Description:
    Quick Load Purple 2 log DNA Ladder 0 1 10 0 kb 750 gel lanes
    Catalog Number:
    n0550l
    Price:
    184
    Size:
    750 gel lanes
    Category:
    DNA Ladders
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    Structured Review

    New England Biolabs quick load purple 1 kb plus dna ladder
    Quick Load Purple 1 kb Plus DNA Ladder
    Quick Load Purple 2 log DNA Ladder 0 1 10 0 kb 750 gel lanes
    https://www.bioz.com/result/quick load purple 1 kb plus dna ladder/product/New England Biolabs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quick load purple 1 kb plus dna ladder - by Bioz Stars, 2020-02
    92/100 stars

    Related Products / Commonly Used Together

    zymoclean gel dna recovery kit
    neb quick-load purple

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    Related Articles

    DNA Extraction:

    Article Title: Genome sequence and plasmid transformation of the model high-yield bacterial cellulose producer Gluconacetobacter hansenii ATCC 53582
    Article Snippet: For plasmid DNA extraction, single colonies were picked from plates after transformation with pSEVA331Bb and inoculated into 5 mL of HS media with 2% (w/v) glucose and 0.2% (v/v) cellulase in a 50 mL tube and incubated at 30 °C with shaking for 48 hours. .. The ladder used was NEB Quick-Load Purple 2-log DNA ladder (cat. no. N0550S – NEB) for all tests.

    Article Title: A Stable Genetic Transformation System and Implications of the Type IV Restriction System in the Nitrogen-Fixing Plant Endosymbiont Frankia alni ACN14a
    Article Snippet: Paragraph title: DNA Extraction ... Plasmids were further purified by running on a 0.7% agarose gel, measured against Quick-Load® Purple 1 kb Plus DNA Ladder (catalog #N0550S, New England Biolabs), and extracted from the gel with a Zymoclean Gel DNA Recovery Kit (Catalog #11-300, Genesee Scientific, San Diego, CA, United States).

    Clone Assay:

    Article Title: Genome sequence and plasmid transformation of the model high-yield bacterial cellulose producer Gluconacetobacter hansenii ATCC 53582
    Article Snippet: Paragraph title: Molecular biology and cloning ... The ladder used was NEB Quick-Load Purple 2-log DNA ladder (cat. no. N0550S – NEB) for all tests.

    Amplification:

    Article Title: A simplified workflow for monoclonal antibody sequencing
    Article Snippet: Paragraph title: PCR amplification of antibody variable regions ... The Quick Load Purple 2-Log DNA Ladder (NEB, N0550S) was used as the standard.

    Agarose Gel Electrophoresis:

    Article Title: A simplified workflow for monoclonal antibody sequencing
    Article Snippet: 5 μL of each RT-PCR reaction was run on a 1% agarose gel in TAE buffer at 90 V. The amplified mouse antibody products appeared between 550–600 base pairs. .. The Quick Load Purple 2-Log DNA Ladder (NEB, N0550S) was used as the standard.

    Article Title: A Stable Genetic Transformation System and Implications of the Type IV Restriction System in the Nitrogen-Fixing Plant Endosymbiont Frankia alni ACN14a
    Article Snippet: .. Plasmids were further purified by running on a 0.7% agarose gel, measured against Quick-Load® Purple 1 kb Plus DNA Ladder (catalog #N0550S, New England Biolabs), and extracted from the gel with a Zymoclean Gel DNA Recovery Kit (Catalog #11-300, Genesee Scientific, San Diego, CA, United States). .. To synthesize unmethylated plasmids, methylated plasmids were first extracted from E. coli DH5α and transformed into E. coli GM48 by heat shock in CaCl2 ( ).

    Methylation:

    Article Title: A Stable Genetic Transformation System and Implications of the Type IV Restriction System in the Nitrogen-Fixing Plant Endosymbiont Frankia alni ACN14a
    Article Snippet: Plasmids were further purified by running on a 0.7% agarose gel, measured against Quick-Load® Purple 1 kb Plus DNA Ladder (catalog #N0550S, New England Biolabs), and extracted from the gel with a Zymoclean Gel DNA Recovery Kit (Catalog #11-300, Genesee Scientific, San Diego, CA, United States). .. To synthesize unmethylated plasmids, methylated plasmids were first extracted from E. coli DH5α and transformed into E. coli GM48 by heat shock in CaCl2 ( ).

    Synthesized:

    Article Title: A simplified workflow for monoclonal antibody sequencing
    Article Snippet: PCR reactions for each cDNA synthesis were set up: 10 μL 5x PCR buffer, 1 μL 10 mM dNTPs, 3 μL synthesized cDNA from the RT reaction, 2.5 μL 10 μM universal forward primer ISPCR, 2.5 μL 10 μM reverse PCR primer based on antibody chain (e.g. mIGK PCR, mIGL PCR, or mIGHG PCR for mouse antibodies), 30.5 μL H2 O, and 0.5 μL 2 U/μL Phusion polymerase (or other high-fidelity polymerase). .. The Quick Load Purple 2-Log DNA Ladder (NEB, N0550S) was used as the standard.

    Spectrophotometry:

    Article Title: A Stable Genetic Transformation System and Implications of the Type IV Restriction System in the Nitrogen-Fixing Plant Endosymbiont Frankia alni ACN14a
    Article Snippet: Plasmids were eluted in EB buffer (Qiagen) and quantified on a NanoDrop Microvolume Spectrophotometer (ThermoFisher). .. Plasmids were further purified by running on a 0.7% agarose gel, measured against Quick-Load® Purple 1 kb Plus DNA Ladder (catalog #N0550S, New England Biolabs), and extracted from the gel with a Zymoclean Gel DNA Recovery Kit (Catalog #11-300, Genesee Scientific, San Diego, CA, United States).

    Purification:

    Article Title: A Stable Genetic Transformation System and Implications of the Type IV Restriction System in the Nitrogen-Fixing Plant Endosymbiont Frankia alni ACN14a
    Article Snippet: .. Plasmids were further purified by running on a 0.7% agarose gel, measured against Quick-Load® Purple 1 kb Plus DNA Ladder (catalog #N0550S, New England Biolabs), and extracted from the gel with a Zymoclean Gel DNA Recovery Kit (Catalog #11-300, Genesee Scientific, San Diego, CA, United States). .. To synthesize unmethylated plasmids, methylated plasmids were first extracted from E. coli DH5α and transformed into E. coli GM48 by heat shock in CaCl2 ( ).

    Polymerase Chain Reaction:

    Article Title: Genome sequence and plasmid transformation of the model high-yield bacterial cellulose producer Gluconacetobacter hansenii ATCC 53582
    Article Snippet: Thermocycler programs for both colony and long-range PCR are listed in . .. The ladder used was NEB Quick-Load Purple 2-log DNA ladder (cat. no. N0550S – NEB) for all tests.

    Article Title: A simplified workflow for monoclonal antibody sequencing
    Article Snippet: Paragraph title: PCR amplification of antibody variable regions ... The Quick Load Purple 2-Log DNA Ladder (NEB, N0550S) was used as the standard.

    Incubation:

    Article Title: Genome sequence and plasmid transformation of the model high-yield bacterial cellulose producer Gluconacetobacter hansenii ATCC 53582
    Article Snippet: For plasmid DNA extraction, single colonies were picked from plates after transformation with pSEVA331Bb and inoculated into 5 mL of HS media with 2% (w/v) glucose and 0.2% (v/v) cellulase in a 50 mL tube and incubated at 30 °C with shaking for 48 hours. .. The ladder used was NEB Quick-Load Purple 2-log DNA ladder (cat. no. N0550S – NEB) for all tests.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: A simplified workflow for monoclonal antibody sequencing
    Article Snippet: 5 μL of each RT-PCR reaction was run on a 1% agarose gel in TAE buffer at 90 V. The amplified mouse antibody products appeared between 550–600 base pairs. .. The Quick Load Purple 2-Log DNA Ladder (NEB, N0550S) was used as the standard.

    Transformation Assay:

    Article Title: Genome sequence and plasmid transformation of the model high-yield bacterial cellulose producer Gluconacetobacter hansenii ATCC 53582
    Article Snippet: For plasmid DNA extraction, single colonies were picked from plates after transformation with pSEVA331Bb and inoculated into 5 mL of HS media with 2% (w/v) glucose and 0.2% (v/v) cellulase in a 50 mL tube and incubated at 30 °C with shaking for 48 hours. .. The ladder used was NEB Quick-Load Purple 2-log DNA ladder (cat. no. N0550S – NEB) for all tests.

    Article Title: A Stable Genetic Transformation System and Implications of the Type IV Restriction System in the Nitrogen-Fixing Plant Endosymbiont Frankia alni ACN14a
    Article Snippet: Plasmids were further purified by running on a 0.7% agarose gel, measured against Quick-Load® Purple 1 kb Plus DNA Ladder (catalog #N0550S, New England Biolabs), and extracted from the gel with a Zymoclean Gel DNA Recovery Kit (Catalog #11-300, Genesee Scientific, San Diego, CA, United States). .. To synthesize unmethylated plasmids, methylated plasmids were first extracted from E. coli DH5α and transformed into E. coli GM48 by heat shock in CaCl2 ( ).

    Plasmid Preparation:

    Article Title: Genome sequence and plasmid transformation of the model high-yield bacterial cellulose producer Gluconacetobacter hansenii ATCC 53582
    Article Snippet: Plasmid DNA was then extracted from the cultures using a QIAprep spin miniprep kit (Qiagen N.V.) according to manufacturer’s instructions. .. The ladder used was NEB Quick-Load Purple 2-log DNA ladder (cat. no. N0550S – NEB) for all tests.

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  • 92
    New England Biolabs quick load purple 1 kb plus dna ladder
    Quick Load Purple 1 Kb Plus Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quick load purple 1 kb plus dna ladder/product/New England Biolabs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quick load purple 1 kb plus dna ladder - by Bioz Stars, 2020-02
    92/100 stars
      Buy from Supplier

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