quick load 100 bp dna ladder  (New England Biolabs)


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    Name:
    Quick Load 100 bp DNA Ladder
    Description:
    Quick Load 100 bp DNA Ladder 375 gel lanes
    Catalog Number:
    n0467l
    Price:
    214
    Size:
    375 gel lanes
    Category:
    DNA Ladders
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    Structured Review

    New England Biolabs quick load 100 bp dna ladder
    Quick Load 100 bp DNA Ladder
    Quick Load 100 bp DNA Ladder 375 gel lanes
    https://www.bioz.com/result/quick load 100 bp dna ladder/product/New England Biolabs
    Average 94 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    quick load 100 bp dna ladder - by Bioz Stars, 2021-02
    94/100 stars

    Images

    1) Product Images from "Development of a Multiplex PCR Assay for Rapid Molecular Serotyping of Haemophilus parasuis"

    Article Title: Development of a Multiplex PCR Assay for Rapid Molecular Serotyping of Haemophilus parasuis

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.01991-15

    Band patterns for the molecular serotyping PCR for all 15 serovars of H. parasuis . Lane M, Quick-Load 100-bp DNA ladder (NEB); S1 to S15 represent the 15 serovars of H. parasuis . Sp-sp, species-specific marker.
    Figure Legend Snippet: Band patterns for the molecular serotyping PCR for all 15 serovars of H. parasuis . Lane M, Quick-Load 100-bp DNA ladder (NEB); S1 to S15 represent the 15 serovars of H. parasuis . Sp-sp, species-specific marker.

    Techniques Used: Polymerase Chain Reaction, Marker

    Determination of the limit of detection for the serotyping multiplex based on pure genomic DNA for the reference strains of serovars 1, 2, 5, 8, 9, and 13. The unit of genomes per microliter is used. Lane M, Quick-Load 100-bp marker (NEB) and H 2 O as the
    Figure Legend Snippet: Determination of the limit of detection for the serotyping multiplex based on pure genomic DNA for the reference strains of serovars 1, 2, 5, 8, 9, and 13. The unit of genomes per microliter is used. Lane M, Quick-Load 100-bp marker (NEB) and H 2 O as the

    Techniques Used: Multiplex Assay, Marker

    2) Product Images from "Developing transgenic wheat to encounter rusts and powdery mildew by overexpressing barley chi26 gene for fungal resistance"

    Article Title: Developing transgenic wheat to encounter rusts and powdery mildew by overexpressing barley chi26 gene for fungal resistance

    Journal: Plant Methods

    doi: 10.1186/s13007-017-0191-5

    PCR products of chi26 (800 bp) ( a ) and bar (400 bp) ( b ) genes from the 14 independent T0 transgenic lines (CHI 1, CHI 3, CHI 6, CHI 7, CHI 9, CHI 10, CHI 14, CHI 16, CHI 20, CHI 22, CHI 30, CHI 47, CHI 50, and CHI 71, lanes 1–14 , respectively). M: Quick-Load ® 100 bp DNA Ladder (New England Biolabs), +: positive control (pBarley/chi/bar), −: negative control (non-transgenic, cv. Hi-Line)
    Figure Legend Snippet: PCR products of chi26 (800 bp) ( a ) and bar (400 bp) ( b ) genes from the 14 independent T0 transgenic lines (CHI 1, CHI 3, CHI 6, CHI 7, CHI 9, CHI 10, CHI 14, CHI 16, CHI 20, CHI 22, CHI 30, CHI 47, CHI 50, and CHI 71, lanes 1–14 , respectively). M: Quick-Load ® 100 bp DNA Ladder (New England Biolabs), +: positive control (pBarley/chi/bar), −: negative control (non-transgenic, cv. Hi-Line)

    Techniques Used: Polymerase Chain Reaction, Transgenic Assay, Positive Control, Negative Control

    3) Product Images from "“Pathotyping” Multiplex PCR Assay for Haemophilus parasuis: a Tool for Prediction of Virulence"

    Article Title: “Pathotyping” Multiplex PCR Assay for Haemophilus parasuis: a Tool for Prediction of Virulence

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.02464-16

    Negative-control panel showing specificity of the primer sets across a range of commensal and pathogenic bacteria of the pig respiratory tract ( Haemophilus parasuis positive controls serovar 2 (SW140) and serovar 5 (Nagasaki), Actinobacillus minor , A. porcinus , A. indolicus , Streptococcus suis , A. pleuropneumoniae , and Bordetella bronchiseptica ). M, Quick-load 100 bp DNA ladder.
    Figure Legend Snippet: Negative-control panel showing specificity of the primer sets across a range of commensal and pathogenic bacteria of the pig respiratory tract ( Haemophilus parasuis positive controls serovar 2 (SW140) and serovar 5 (Nagasaki), Actinobacillus minor , A. porcinus , A. indolicus , Streptococcus suis , A. pleuropneumoniae , and Bordetella bronchiseptica ). M, Quick-load 100 bp DNA ladder.

    Techniques Used: Negative Control

    4) Product Images from "A Novel Multiplex PCR-RFLP Method for Simultaneous Genotyping of CYP3A4*4 A>G, CYP3A4*18B G>A and CYP3A4*22 C>T"

    Article Title: A Novel Multiplex PCR-RFLP Method for Simultaneous Genotyping of CYP3A4*4 A>G, CYP3A4*18B G>A and CYP3A4*22 C>T

    Journal: The Malaysian Journal of Medical Sciences : MJMS

    doi: 10.21315/mjms2018.25.4.7

    A 2% agarose gel showing PCR products from multiplex and uniplex PCR for CYP3A4*4 , CYP3A4*18B and CYP3A4*22 . L1: Quick-Load 100bp DNA ladder (NEB® inc, Massachusetts, USA). L2: multiplex pcr with band sizes of 244 bp, 331 bp and 793 bp. L3, L4 and L5 contain positive controls for CYP3A4*4 with 244 bp, CYP3A4*18B with 331 bp and CYP3A4*22 with 793 bp respectively. L6 is a negative control
    Figure Legend Snippet: A 2% agarose gel showing PCR products from multiplex and uniplex PCR for CYP3A4*4 , CYP3A4*18B and CYP3A4*22 . L1: Quick-Load 100bp DNA ladder (NEB® inc, Massachusetts, USA). L2: multiplex pcr with band sizes of 244 bp, 331 bp and 793 bp. L3, L4 and L5 contain positive controls for CYP3A4*4 with 244 bp, CYP3A4*18B with 331 bp and CYP3A4*22 with 793 bp respectively. L6 is a negative control

    Techniques Used: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Multiplex Assay, Negative Control

    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: Development of a Multiplex PCR Assay for Rapid Molecular Serotyping of Haemophilus parasuis
    Article Snippet: .. Gel electrophoresis was performed in a 2.0% agarose gel in 1× Tris-borate-EDTA (TBE) with 5% SYBR Safe dye (Invitrogen) at 120 V for 50 min using the Quick-Load 100-bp DNA ladder (New England BioLabs) as the molecular size standard. .. All results were analyzed using a GelDoc XR imager (Bio-Rad).

    Article Title: “Pathotyping” Multiplex PCR Assay for Haemophilus parasuis: a Tool for Prediction of Virulence
    Article Snippet: .. Gel electrophoresis was performed using 2.0% agarose gel with 5% SybrSafe dye (Invitrogen) and run at 110 V for 90 min using the Quick-load 100 bp DNA ladder (New England BioLabs) for all mPCRs. .. All mPCR results were visualized using the GelDoc imager (Bio-Rad).

    Multiplex Assay:

    Article Title: A Novel Multiplex PCR-RFLP Method for Simultaneous Genotyping of CYP3A4*4 A>G, CYP3A4*18B G>A and CYP3A4*22 C>T
    Article Snippet: .. For confirmation of uniplex and multiplex PCR , 1.0 μL of 6× DNA loading dye® (Thermo Fisher Scientific Inc, Massachusetts, USA) was mixed with 1.0 μL of SYBR® Green I stain (Lonza, Rockland, USA) and either 2.0 μL of a Quick-Load 100bp DNA ladder (NEB® Inc, Massachusetts, USA) or 2.0 μL of the PCR products on a Parafilm® (Parafilm, Bemis, USA); the mixture was then loaded into the well and then electrophoresed on 2% agarose at 100 V for 45 min. On the other hand, for multiplex PCRRFLP , 1.0 μL of 6× DNA loading dye® (Thermo Fisher Scientific Inc, Massachusetts, USA) was mixed with 1.0 μL of SYBR® Green I stain (Lonza, Rockland, USA) and either 1.0 μL of a 50 bp DNA ladder (NEB® Inc, Massachusetts, USA) or 2.0 μL of PCR products on a Parafilm® (Parafilm, Bemis, USA); the mixture was then loaded into a 4% agarose and electrophoresed at 100 V for 1.5 h. All gels were visualised using Alpha Innotech® Ultraviolet Transilluminator (Alpha Innotech® USA). .. PCR Products Purification and DNA Sequencing Prior to sequencing, the PCR products were purified using illustraTM ExoProsterTM 1-Step Enzymatic and Sequencing Clean-Up (GE HealthCare Life Sciences, UK) according to manufacturer’s instructions.

    Amplification:

    Article Title: Anisakid nematode species identification in harbour porpoises (Phocoena phocoena) from the North Sea, Baltic Sea and North Atlantic using RFLP analysis
    Article Snippet: .. In order to verify the amplification quality, 10 μl of the PCR products were visualised by ultraviolet light (monitor: Santec Peqlab, Peqlab (VWR), Erlangen, Germany; darkroom: Vilber Lourmat Deutschland GmbH, Eberhardzell, Germany) on SYBR® Safe DNA (Invitrogen (Thermo Fisher Scientific), Waltham, USA) (1.5 μl) stained 1.5% agarose gels (UltraPure™ Agarose, Invitrogen (Thermo Fisher Scientific), Waltham, USA), using a 100-bp DNA ladder (4 μl) (Quick-Load®, New England Biolabs GmbH, Frankfurt am Main, Germany) as molecular marker. .. 2.3 RFLP The PCR products were digested using the restriction enzymes Hinf I, Rsa I and Hae III.

    Agarose Gel Electrophoresis:

    Article Title: Role of a Dual Splicing and Amino Acid Code in Myopia, Cone Dysfunction and Cone Dystrophy Associated with L/M Opsin Interchange Mutations
    Article Snippet: .. The PCR product was analyzed by agarose gel electrophoresis using the Quickload 100bp Ladder (New England Biolabs, Inc., Ipswich, MA) as molecular weight markers. ..

    Article Title: Development of a Multiplex PCR Assay for Rapid Molecular Serotyping of Haemophilus parasuis
    Article Snippet: .. Gel electrophoresis was performed in a 2.0% agarose gel in 1× Tris-borate-EDTA (TBE) with 5% SYBR Safe dye (Invitrogen) at 120 V for 50 min using the Quick-Load 100-bp DNA ladder (New England BioLabs) as the molecular size standard. .. All results were analyzed using a GelDoc XR imager (Bio-Rad).

    Article Title: “Pathotyping” Multiplex PCR Assay for Haemophilus parasuis: a Tool for Prediction of Virulence
    Article Snippet: .. Gel electrophoresis was performed using 2.0% agarose gel with 5% SybrSafe dye (Invitrogen) and run at 110 V for 90 min using the Quick-load 100 bp DNA ladder (New England BioLabs) for all mPCRs. .. All mPCR results were visualized using the GelDoc imager (Bio-Rad).

    SYBR Green Assay:

    Article Title: A Novel Multiplex PCR-RFLP Method for Simultaneous Genotyping of CYP3A4*4 A>G, CYP3A4*18B G>A and CYP3A4*22 C>T
    Article Snippet: .. For confirmation of uniplex and multiplex PCR , 1.0 μL of 6× DNA loading dye® (Thermo Fisher Scientific Inc, Massachusetts, USA) was mixed with 1.0 μL of SYBR® Green I stain (Lonza, Rockland, USA) and either 2.0 μL of a Quick-Load 100bp DNA ladder (NEB® Inc, Massachusetts, USA) or 2.0 μL of the PCR products on a Parafilm® (Parafilm, Bemis, USA); the mixture was then loaded into the well and then electrophoresed on 2% agarose at 100 V for 45 min. On the other hand, for multiplex PCRRFLP , 1.0 μL of 6× DNA loading dye® (Thermo Fisher Scientific Inc, Massachusetts, USA) was mixed with 1.0 μL of SYBR® Green I stain (Lonza, Rockland, USA) and either 1.0 μL of a 50 bp DNA ladder (NEB® Inc, Massachusetts, USA) or 2.0 μL of PCR products on a Parafilm® (Parafilm, Bemis, USA); the mixture was then loaded into a 4% agarose and electrophoresed at 100 V for 1.5 h. All gels were visualised using Alpha Innotech® Ultraviolet Transilluminator (Alpha Innotech® USA). .. PCR Products Purification and DNA Sequencing Prior to sequencing, the PCR products were purified using illustraTM ExoProsterTM 1-Step Enzymatic and Sequencing Clean-Up (GE HealthCare Life Sciences, UK) according to manufacturer’s instructions.

    Polymerase Chain Reaction:

    Article Title: A Novel Multiplex PCR-RFLP Method for Simultaneous Genotyping of CYP3A4*4 A>G, CYP3A4*18B G>A and CYP3A4*22 C>T
    Article Snippet: .. For confirmation of uniplex and multiplex PCR , 1.0 μL of 6× DNA loading dye® (Thermo Fisher Scientific Inc, Massachusetts, USA) was mixed with 1.0 μL of SYBR® Green I stain (Lonza, Rockland, USA) and either 2.0 μL of a Quick-Load 100bp DNA ladder (NEB® Inc, Massachusetts, USA) or 2.0 μL of the PCR products on a Parafilm® (Parafilm, Bemis, USA); the mixture was then loaded into the well and then electrophoresed on 2% agarose at 100 V for 45 min. On the other hand, for multiplex PCRRFLP , 1.0 μL of 6× DNA loading dye® (Thermo Fisher Scientific Inc, Massachusetts, USA) was mixed with 1.0 μL of SYBR® Green I stain (Lonza, Rockland, USA) and either 1.0 μL of a 50 bp DNA ladder (NEB® Inc, Massachusetts, USA) or 2.0 μL of PCR products on a Parafilm® (Parafilm, Bemis, USA); the mixture was then loaded into a 4% agarose and electrophoresed at 100 V for 1.5 h. All gels were visualised using Alpha Innotech® Ultraviolet Transilluminator (Alpha Innotech® USA). .. PCR Products Purification and DNA Sequencing Prior to sequencing, the PCR products were purified using illustraTM ExoProsterTM 1-Step Enzymatic and Sequencing Clean-Up (GE HealthCare Life Sciences, UK) according to manufacturer’s instructions.

    Article Title: Role of a Dual Splicing and Amino Acid Code in Myopia, Cone Dysfunction and Cone Dystrophy Associated with L/M Opsin Interchange Mutations
    Article Snippet: .. The PCR product was analyzed by agarose gel electrophoresis using the Quickload 100bp Ladder (New England Biolabs, Inc., Ipswich, MA) as molecular weight markers. ..

    Article Title: Genetic variations among three major ethnic groups in Nigeria using RAPD
    Article Snippet: .. Agarose from Cleaver Scientific Limited, Quickload 100bp DNA ladder from New England Biolabs Inc., PCR Master-mix with standard buffer (Quick-Ld2X) containing Taq polymerase was also from New England Biolabs Inc. and the oligonucleotide primers were from Inqaba, South Africa. ..

    Article Title: Anisakid nematode species identification in harbour porpoises (Phocoena phocoena) from the North Sea, Baltic Sea and North Atlantic using RFLP analysis
    Article Snippet: .. In order to verify the amplification quality, 10 μl of the PCR products were visualised by ultraviolet light (monitor: Santec Peqlab, Peqlab (VWR), Erlangen, Germany; darkroom: Vilber Lourmat Deutschland GmbH, Eberhardzell, Germany) on SYBR® Safe DNA (Invitrogen (Thermo Fisher Scientific), Waltham, USA) (1.5 μl) stained 1.5% agarose gels (UltraPure™ Agarose, Invitrogen (Thermo Fisher Scientific), Waltham, USA), using a 100-bp DNA ladder (4 μl) (Quick-Load®, New England Biolabs GmbH, Frankfurt am Main, Germany) as molecular marker. .. 2.3 RFLP The PCR products were digested using the restriction enzymes Hinf I, Rsa I and Hae III.

    Marker:

    Article Title: Anisakid nematode species identification in harbour porpoises (Phocoena phocoena) from the North Sea, Baltic Sea and North Atlantic using RFLP analysis
    Article Snippet: .. In order to verify the amplification quality, 10 μl of the PCR products were visualised by ultraviolet light (monitor: Santec Peqlab, Peqlab (VWR), Erlangen, Germany; darkroom: Vilber Lourmat Deutschland GmbH, Eberhardzell, Germany) on SYBR® Safe DNA (Invitrogen (Thermo Fisher Scientific), Waltham, USA) (1.5 μl) stained 1.5% agarose gels (UltraPure™ Agarose, Invitrogen (Thermo Fisher Scientific), Waltham, USA), using a 100-bp DNA ladder (4 μl) (Quick-Load®, New England Biolabs GmbH, Frankfurt am Main, Germany) as molecular marker. .. 2.3 RFLP The PCR products were digested using the restriction enzymes Hinf I, Rsa I and Hae III.

    Staining:

    Article Title: A Novel Multiplex PCR-RFLP Method for Simultaneous Genotyping of CYP3A4*4 A>G, CYP3A4*18B G>A and CYP3A4*22 C>T
    Article Snippet: .. For confirmation of uniplex and multiplex PCR , 1.0 μL of 6× DNA loading dye® (Thermo Fisher Scientific Inc, Massachusetts, USA) was mixed with 1.0 μL of SYBR® Green I stain (Lonza, Rockland, USA) and either 2.0 μL of a Quick-Load 100bp DNA ladder (NEB® Inc, Massachusetts, USA) or 2.0 μL of the PCR products on a Parafilm® (Parafilm, Bemis, USA); the mixture was then loaded into the well and then electrophoresed on 2% agarose at 100 V for 45 min. On the other hand, for multiplex PCRRFLP , 1.0 μL of 6× DNA loading dye® (Thermo Fisher Scientific Inc, Massachusetts, USA) was mixed with 1.0 μL of SYBR® Green I stain (Lonza, Rockland, USA) and either 1.0 μL of a 50 bp DNA ladder (NEB® Inc, Massachusetts, USA) or 2.0 μL of PCR products on a Parafilm® (Parafilm, Bemis, USA); the mixture was then loaded into a 4% agarose and electrophoresed at 100 V for 1.5 h. All gels were visualised using Alpha Innotech® Ultraviolet Transilluminator (Alpha Innotech® USA). .. PCR Products Purification and DNA Sequencing Prior to sequencing, the PCR products were purified using illustraTM ExoProsterTM 1-Step Enzymatic and Sequencing Clean-Up (GE HealthCare Life Sciences, UK) according to manufacturer’s instructions.

    Article Title: Anisakid nematode species identification in harbour porpoises (Phocoena phocoena) from the North Sea, Baltic Sea and North Atlantic using RFLP analysis
    Article Snippet: .. In order to verify the amplification quality, 10 μl of the PCR products were visualised by ultraviolet light (monitor: Santec Peqlab, Peqlab (VWR), Erlangen, Germany; darkroom: Vilber Lourmat Deutschland GmbH, Eberhardzell, Germany) on SYBR® Safe DNA (Invitrogen (Thermo Fisher Scientific), Waltham, USA) (1.5 μl) stained 1.5% agarose gels (UltraPure™ Agarose, Invitrogen (Thermo Fisher Scientific), Waltham, USA), using a 100-bp DNA ladder (4 μl) (Quick-Load®, New England Biolabs GmbH, Frankfurt am Main, Germany) as molecular marker. .. 2.3 RFLP The PCR products were digested using the restriction enzymes Hinf I, Rsa I and Hae III.

    Molecular Weight:

    Article Title: Role of a Dual Splicing and Amino Acid Code in Myopia, Cone Dysfunction and Cone Dystrophy Associated with L/M Opsin Interchange Mutations
    Article Snippet: .. The PCR product was analyzed by agarose gel electrophoresis using the Quickload 100bp Ladder (New England Biolabs, Inc., Ipswich, MA) as molecular weight markers. ..

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    New England Biolabs quick load 100 bp dna ladder
    Band patterns for the molecular serotyping PCR for all 15 serovars of H. parasuis . Lane M, Quick-Load 100-bp <t>DNA</t> ladder (NEB); S1 to S15 represent the 15 serovars of H. parasuis . Sp-sp, species-specific marker.
    Quick Load 100 Bp Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quick load 100 bp dna ladder/product/New England Biolabs
    Average 94 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    quick load 100 bp dna ladder - by Bioz Stars, 2021-02
    94/100 stars
      Buy from Supplier

    Image Search Results


    Band patterns for the molecular serotyping PCR for all 15 serovars of H. parasuis . Lane M, Quick-Load 100-bp DNA ladder (NEB); S1 to S15 represent the 15 serovars of H. parasuis . Sp-sp, species-specific marker.

    Journal: Journal of Clinical Microbiology

    Article Title: Development of a Multiplex PCR Assay for Rapid Molecular Serotyping of Haemophilus parasuis

    doi: 10.1128/JCM.01991-15

    Figure Lengend Snippet: Band patterns for the molecular serotyping PCR for all 15 serovars of H. parasuis . Lane M, Quick-Load 100-bp DNA ladder (NEB); S1 to S15 represent the 15 serovars of H. parasuis . Sp-sp, species-specific marker.

    Article Snippet: Gel electrophoresis was performed in a 2.0% agarose gel in 1× Tris-borate-EDTA (TBE) with 5% SYBR Safe dye (Invitrogen) at 120 V for 50 min using the Quick-Load 100-bp DNA ladder (New England BioLabs) as the molecular size standard.

    Techniques: Polymerase Chain Reaction, Marker

    Determination of the limit of detection for the serotyping multiplex based on pure genomic DNA for the reference strains of serovars 1, 2, 5, 8, 9, and 13. The unit of genomes per microliter is used. Lane M, Quick-Load 100-bp marker (NEB) and H 2 O as the

    Journal: Journal of Clinical Microbiology

    Article Title: Development of a Multiplex PCR Assay for Rapid Molecular Serotyping of Haemophilus parasuis

    doi: 10.1128/JCM.01991-15

    Figure Lengend Snippet: Determination of the limit of detection for the serotyping multiplex based on pure genomic DNA for the reference strains of serovars 1, 2, 5, 8, 9, and 13. The unit of genomes per microliter is used. Lane M, Quick-Load 100-bp marker (NEB) and H 2 O as the

    Article Snippet: Gel electrophoresis was performed in a 2.0% agarose gel in 1× Tris-borate-EDTA (TBE) with 5% SYBR Safe dye (Invitrogen) at 120 V for 50 min using the Quick-Load 100-bp DNA ladder (New England BioLabs) as the molecular size standard.

    Techniques: Multiplex Assay, Marker

    PCR products of chi26 (800 bp) ( a ) and bar (400 bp) ( b ) genes from the 14 independent T0 transgenic lines (CHI 1, CHI 3, CHI 6, CHI 7, CHI 9, CHI 10, CHI 14, CHI 16, CHI 20, CHI 22, CHI 30, CHI 47, CHI 50, and CHI 71, lanes 1–14 , respectively). M: Quick-Load ® 100 bp DNA Ladder (New England Biolabs), +: positive control (pBarley/chi/bar), −: negative control (non-transgenic, cv. Hi-Line)

    Journal: Plant Methods

    Article Title: Developing transgenic wheat to encounter rusts and powdery mildew by overexpressing barley chi26 gene for fungal resistance

    doi: 10.1186/s13007-017-0191-5

    Figure Lengend Snippet: PCR products of chi26 (800 bp) ( a ) and bar (400 bp) ( b ) genes from the 14 independent T0 transgenic lines (CHI 1, CHI 3, CHI 6, CHI 7, CHI 9, CHI 10, CHI 14, CHI 16, CHI 20, CHI 22, CHI 30, CHI 47, CHI 50, and CHI 71, lanes 1–14 , respectively). M: Quick-Load ® 100 bp DNA Ladder (New England Biolabs), +: positive control (pBarley/chi/bar), −: negative control (non-transgenic, cv. Hi-Line)

    Article Snippet: Quick-Load 100 bp DNA ladder (New England Biolabs) was used as a DNA standard.

    Techniques: Polymerase Chain Reaction, Transgenic Assay, Positive Control, Negative Control

    Negative-control panel showing specificity of the primer sets across a range of commensal and pathogenic bacteria of the pig respiratory tract ( Haemophilus parasuis positive controls serovar 2 (SW140) and serovar 5 (Nagasaki), Actinobacillus minor , A. porcinus , A. indolicus , Streptococcus suis , A. pleuropneumoniae , and Bordetella bronchiseptica ). M, Quick-load 100 bp DNA ladder.

    Journal: Journal of Clinical Microbiology

    Article Title: “Pathotyping” Multiplex PCR Assay for Haemophilus parasuis: a Tool for Prediction of Virulence

    doi: 10.1128/JCM.02464-16

    Figure Lengend Snippet: Negative-control panel showing specificity of the primer sets across a range of commensal and pathogenic bacteria of the pig respiratory tract ( Haemophilus parasuis positive controls serovar 2 (SW140) and serovar 5 (Nagasaki), Actinobacillus minor , A. porcinus , A. indolicus , Streptococcus suis , A. pleuropneumoniae , and Bordetella bronchiseptica ). M, Quick-load 100 bp DNA ladder.

    Article Snippet: Gel electrophoresis was performed using 2.0% agarose gel with 5% SybrSafe dye (Invitrogen) and run at 110 V for 90 min using the Quick-load 100 bp DNA ladder (New England BioLabs) for all mPCRs.

    Techniques: Negative Control

    A 2% agarose gel showing PCR products from multiplex and uniplex PCR for CYP3A4*4 , CYP3A4*18B and CYP3A4*22 . L1: Quick-Load 100bp DNA ladder (NEB® inc, Massachusetts, USA). L2: multiplex pcr with band sizes of 244 bp, 331 bp and 793 bp. L3, L4 and L5 contain positive controls for CYP3A4*4 with 244 bp, CYP3A4*18B with 331 bp and CYP3A4*22 with 793 bp respectively. L6 is a negative control

    Journal: The Malaysian Journal of Medical Sciences : MJMS

    Article Title: A Novel Multiplex PCR-RFLP Method for Simultaneous Genotyping of CYP3A4*4 A>G, CYP3A4*18B G>A and CYP3A4*22 C>T

    doi: 10.21315/mjms2018.25.4.7

    Figure Lengend Snippet: A 2% agarose gel showing PCR products from multiplex and uniplex PCR for CYP3A4*4 , CYP3A4*18B and CYP3A4*22 . L1: Quick-Load 100bp DNA ladder (NEB® inc, Massachusetts, USA). L2: multiplex pcr with band sizes of 244 bp, 331 bp and 793 bp. L3, L4 and L5 contain positive controls for CYP3A4*4 with 244 bp, CYP3A4*18B with 331 bp and CYP3A4*22 with 793 bp respectively. L6 is a negative control

    Article Snippet: For confirmation of uniplex and multiplex PCR , 1.0 μL of 6× DNA loading dye® (Thermo Fisher Scientific Inc, Massachusetts, USA) was mixed with 1.0 μL of SYBR® Green I stain (Lonza, Rockland, USA) and either 2.0 μL of a Quick-Load 100bp DNA ladder (NEB® Inc, Massachusetts, USA) or 2.0 μL of the PCR products on a Parafilm® (Parafilm, Bemis, USA); the mixture was then loaded into the well and then electrophoresed on 2% agarose at 100 V for 45 min. On the other hand, for multiplex PCRRFLP , 1.0 μL of 6× DNA loading dye® (Thermo Fisher Scientific Inc, Massachusetts, USA) was mixed with 1.0 μL of SYBR® Green I stain (Lonza, Rockland, USA) and either 1.0 μL of a 50 bp DNA ladder (NEB® Inc, Massachusetts, USA) or 2.0 μL of PCR products on a Parafilm® (Parafilm, Bemis, USA); the mixture was then loaded into a 4% agarose and electrophoresed at 100 V for 1.5 h. All gels were visualised using Alpha Innotech® Ultraviolet Transilluminator (Alpha Innotech® USA).

    Techniques: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Multiplex Assay, Negative Control