ribonucleotide  (New England Biolabs)


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    Name:
    Ribonucleotide Solution Mix
    Description:
    Ribonucleotide Solution Mix 50 umol of each
    Catalog Number:
    N0466L
    Price:
    296
    Category:
    Ribonucleotides
    Size:
    50 umol of each
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    Structured Review

    New England Biolabs ribonucleotide
    Ribonucleotide Solution Mix
    Ribonucleotide Solution Mix 50 umol of each
    https://www.bioz.com/result/ribonucleotide/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ribonucleotide - by Bioz Stars, 2021-05
    93/100 stars

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    Related Articles

    Produced:

    Article Title: Single step production of Cas9 mRNA for zygote injection
    Article Snippet: .. Alternatively, an uncapped mRNA was also produced by using the linearized pBKR3 vector and completing in vitro transcription by using a ribonucleotide solution mix (NEB, Ipswich, MA, USA) and an RNA polymerase (NEB), and then the resulting mRNA was purified by using the MEGAclear kit. ..

    Plasmid Preparation:

    Article Title: Single step production of Cas9 mRNA for zygote injection
    Article Snippet: .. Alternatively, an uncapped mRNA was also produced by using the linearized pBKR3 vector and completing in vitro transcription by using a ribonucleotide solution mix (NEB, Ipswich, MA, USA) and an RNA polymerase (NEB), and then the resulting mRNA was purified by using the MEGAclear kit. ..

    In Vitro:

    Article Title: Single step production of Cas9 mRNA for zygote injection
    Article Snippet: .. Alternatively, an uncapped mRNA was also produced by using the linearized pBKR3 vector and completing in vitro transcription by using a ribonucleotide solution mix (NEB, Ipswich, MA, USA) and an RNA polymerase (NEB), and then the resulting mRNA was purified by using the MEGAclear kit. ..

    Purification:

    Article Title: Single step production of Cas9 mRNA for zygote injection
    Article Snippet: .. Alternatively, an uncapped mRNA was also produced by using the linearized pBKR3 vector and completing in vitro transcription by using a ribonucleotide solution mix (NEB, Ipswich, MA, USA) and an RNA polymerase (NEB), and then the resulting mRNA was purified by using the MEGAclear kit. ..

    Staining:

    Article Title: SHERLOCK: Nucleic acid detection with CRISPR nucleases
    Article Snippet: .. Novex™ TBE-Urea Gels, 10% (ThermoFisher Scientific cat. no. EC68755BOX) SYBR™ Gold Nucleic Acid Gel Stain 10,000X Concentrate in DMSO (ThermoFisher Scientific cat. no. S11494) TwistAmp® Basic (TwistDX cat. no. TABAS03KIT) TwistAmp® Basic RT (TwistDX cat. no. TABARTS01kit) RNase Inhibitor, Murine (New England Biolabs cat. no. M0314S) NxGen® T7 RNA Polymerase 125000U (Lucigen cat. No. 30223–2 30223–2) RNaseAlert™ Lab Test Kit v2 (Invitrogen cat. No. 4479768) Ribonucleotide Solution Mix (New England Biolabs cat. No. N0466L) Milenia HybriDetect 1 (TwistDx™ cat. no. MILENIA01) .. Constant temperature incubator (e.g., Quincy Lab, Inc Incubator Model 12–140E) 100 mm × 15 mm Not TC-Treated Bacteriological Petri Dish (Corning, cat. no. 351029) Culture flasks, 1 L (e.g., Corning® 5100–1L PYREX® Wide Mouth flask) Biological shaker (e.g., Eppendorf Innnova S44i) Fisherbrand™ Disposable Cuvettes; Polystyrene (cat. no. 14–955-127) Floor centrifuge with 4 L capacity (e.g., Beckman-Coulter, Avanti J-E) Gel electrophoresis tank and power source (e.g., Invitrogen™ Mini Gel Tank; VWR Power Source 250 V cat. no. 93000–746) LM20 Microfluidizer® from Microfluidics Corning™ Polypropylene Centrifuge Tubes, 250 mL (cat. no. 05–538-53) Tube rotator (e.g., Scilogex MX-RL-Pro, cat. no. 824222019999) Plate shaker (e.g., VWR® Mini Shaker) Chromatography column (e.g., Bio-Rad Glass Econo-Column®) eStain® L1 Protein Staining System (GenScript®) or Coomassie Stain (Coomassie Brilliant Blue R-250, cat. no. 1610400, Bio-Rad) and destain solution (40% methanol, 10% acetic acid) FPLC (e.g., ÄKTA pure by GE Healthcare Life Sciences) LightCycler® 480 Instrument II (Roche Diagnostics, product no. 05015243001) QX200™ droplet generator (Bio-Rad, cat. no. 186–4002) QX200™ droplet reader (Bio-Rad, cat. no. 186–4003) Benchtop centrifuge (e.g., Beckman Coulter Allegra X-15) Axygen™ 8-Strip PCR tubes (Fisher Scientific, cat. no. 14–222-250) Axygen™ PCR plates, 96 well (VWR, cat. no. PCR-96M2-HS-C) 384-well optical plate (e.g., Roche, LightCycler 480 Multiwell plate 384, cat. no. 5102430001) Axygen 1.5 mL Boil-Proof Microcentrifuge Tubes, (VWR, cat. no. 10011–702) Falcon tubes, polypropylene, 15 mL (Corning cat. no. 352097) Falcon tubes, polypropylene, 50 mL (Corning, cat. no. 352070) Eppendorf Safe-lock microcentrifuge tubes, 1.5 mL (Eppendorf, cat. no. T9661–1000EA) Filtered sterile pipette tips (e.g., Rainin) Corning bottle-top vacuum filter system, 0.22 μM (Sigma-Aldrich, cat. no. CLS431098) Stericup filter unit, 0.45 μM (Millipore, cat. no. SCHVU02RE) Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-50 membrane (Sigma-Aldrich, cat. no. UFC905024) Thermocycler with programmable temperature stepping functionality, 96 well (e.g., Applied Biosystems Veriti, cat. no. 4375786) Desktop microcentrifuges (e.g., Eppendorf, cat. no. 5424) Digital gel imaging system (GelDoc EZ, Bio-Rad, cat. no. 170–8270) and white sample tray (Bio-Rad, cat. no. 170–8272) ) UV spectrophotometer (e.g., NanoDrop 2000c, Thermo Scientific) Plate spectrophotometer (e.g., Synergy H4 Hybrid Multi-Mode Microplate Reader, BioTek)

    Binding Assay:

    Article Title: Sensitive fluorescence detection of SARS-CoV-2 RNA in clinical samples via one-pot isothermal ligation and transcription
    Article Snippet: Given the simple probe design process and its rapid development, SENSR will be a suitable diagnostic method for emerging infectious diseases. .. MaterialsSplintR ligase, T7 RNA polymerase, extreme thermostable single-stranded DNA binding protein (ET-SSB), DNase I (RNase-free) and ribonucleotide solution mix were obtained from New England Biolabs. .. Recombinant RNase inhibitor was obtained from Takara.

    Article Title: Sensitive one-step isothermal detection of pathogen-derived RNAs
    Article Snippet: .. SplintR ligase, T7 RNA polymerase, extreme thermostable single-stranded DNA 338 binding protein (ET-SSB), DNase I (RNase-free), and ribonucleotide solution mix were 339 obtained from New England Biolabs (NEB, Ipswich, MA, USA). .. Recombinant RNase 340 Inhibitor and pMD20 T-vector were obtained from Takara (Shiga, Japan).

    CRISPR:

    Article Title: A role for Biofoundries in rapid development and validation of automated SARS-CoV-2 clinical diagnostics
    Article Snippet: Final reactions had a final volume of 14 µL and were set up in Echo 384 LDV Plus plates (final primer concentration of 0.45 µM and 2 µL of purified patient RNA template) and incubated at 42 °C for 30 min in a CLARIOstar Plus (BMG Labtech) plate reader with double orbital shaking of 600 r.p.m. for 30 s at 5 min. All concentrations are final CRISPR reaction concentrations and the final CRISPR reaction volumes were 5 µL. .. An Echo 525 (Labcyte) was used to transfer CRISPR Master Mix (50 µM LwCas13a, 1 U/µL Murine RNase inhibitor (NEB), 4 mM Ribonucleotide Solution Mix (NEB), 1.5 U/µL T7 RNA Polymerase (Thermo Fisher Scientific), and 1.25 ng/µL HEK293F background RNA) in Nuclease Reaction Buffer (20 mM HEPES pH 6.8, 60 mM NaCl, 9 mM MgCl2 ) to a 384-well Small Volume LoBase Microplate (Greiner Bio-One). crRNA (25 nM) and 200 nM poly-U fluorescent probe (5′-/56-FAM/rUrUrUrUrU/3IABkFQ/-3′) were then added separately. .. An Echo 550 (Labcyte) was used to transfer pre-amplified products (0.25 µL) from the 384 LDV Plus Echo plate (Labcyte) to initiate the reaction, the plate was sealed, centrifuged at 500 × g for 1 min and read using a CLARIOstar Plus (BMG Labtech) plate reader with an excitation wavelength of 483-14 nm, emission of 530-30 nm, dichroic filter of 502.5 nm and EDR enabled.

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  • 93
    New England Biolabs ribonucleotide solution mix
    Ribonucleotide Solution Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ribonucleotide solution mix/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ribonucleotide solution mix - by Bioz Stars, 2021-05
    93/100 stars
      Buy from Supplier

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