n0446s  (New England Biolabs)


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    Name:
    Deoxynucleotide dNTP Solution Set
    Description:

    Catalog Number:
    N0446
    Price:
    176
    Category:
    Nucleic Acids
    Applications:
    DNA Analysis
    Size:
    25 µmol
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    Structured Review

    New England Biolabs n0446s
    Deoxynucleotide dNTP Solution Set

    https://www.bioz.com/result/n0446s/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    n0446s - by Bioz Stars, 2021-08
    95/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: DNA cleavage assay for the identification of topoisomerase I inhibitors.
    Article Snippet: .. The inhibition of DNA topoisomerase I (Top1) has proven to be a successful approach in the design of anticancer agents. ..

    Polymerase Chain Reaction:

    Article Title: DNA cleavage assay for the identification of topoisomerase I inhibitors.
    Article Snippet: .. The inhibition of DNA topoisomerase I (Top1) has proven to be a successful approach in the design of anticancer agents. ..

    Article Title: Genetic Screens and Biochemical Assays to Characterize Vibrio cholerae O1 Biotypes: Classical and El Tor
    Article Snippet: .. Overnight Cultures QIAprep Spin Miniprep Kit (Qiagen Cat. #27104) NanoDrop ND100 Spectrophotometer (NanoDrop Model #ND100) 200 μl Sterile Thin-walled PCR Tubes (Corning Cat. #6571) tcpA / ctxB Forward and Reverse Primers 1.25 mM dNTP Mix (NEB Cat. #N0446S) (see recipe) Pfu Turbo Cx HotStart DNA Polymerase (Agilent Cat. #600412-51) 10X Pfu Turbo Cx Reaction Buffer (Stratagene Cat. #600410-52) PCR Thermal Cycler (to hold 200 μl PCR tubes) with Heated Lid UltraPure Agarose (Invitrogen Cat. #16500-500) 1X TBE (see recipe) 6X DNA Gel Loading Dye (see recipe) QIAquick PCR Purification Kit (Qiagen Cat. #28104) Design primers to anneal approximately 50-75 bp upstream and downstream of the translational start and stop sites, respectively, of either tcpA or ctxB . ..

    Article Title: The GLIB technique for genome-wide mapping of 5-hydroxymethylcytosine
    Article Snippet: .. Sodium acetate (Sigma-Aldrich, cat. no. S2889-250G) QIAquick PCR purification kit (Qiagen, cat. no. 28106) QIAquick gel extraction kit (Qiagen, cat. no. 28704) Illustra MicroSpin G-50 columns (GE Healthcare, cat. no. 27-5330-01) Dynabeads M280-Streptavidin (Invitrogen, cat. no. 11206D) QuantIt OliGreen kit (Invitrogen, cat. no. ) PBS, 10× (Meditech CellGro, cat. no. 46-013-CM) Deoxynucleotide solution set (New England Biolabs, cat. no. N0446S) Hydroxymethyl dCTP (Bioline, cat. no. BIO-39046) pUC19 DNA (New England Biolabs, cat. no. N3041S) T4 phage β-glucosyltransferase (T4-BGT; New England Biolabs, cat. no. M0357S) AhdI (New England Biolabs, cat. no. R0584S) FspI (New England Biolabs, cat. no. R0135S) HindIII (New England Biolabs, cat. no. R0104S) NdeI (New England Biolabs, cat. no. R0111S) DNA polymerase, Klenow fragment (New England Biolabs, cat. no. M0210S) Ethanol (Sigma-Aldrich, cat. no. E7023) Tris (Sigma-Aldrich, cat. no. 154563) Agarose (Invitrogen, cat. no. 16500500) ..

    Article Title: Preparation of reduced representation bisulfite sequencing libraries for genome-scale DNA methylation profiling.
    Article Snippet: .. Genome-wide mapping of 5-methylcytosine is of broad interest to many fields of biology and medicine. ..

    Article Title: ATG-18 and EPG-6 are Both Required for Autophagy but Differentially Contribute to Lifespan Control in Caenorhabditis elegans
    Article Snippet: .. For one PCR reaction, 25 units of Taq DNA polymerase (NEB, M0273) with standard Taq buffer (NEB, M0273L), 200 μM dNTP (NEB, N0446S), 0.5 μM of both primers (Sigma–Aldrich, Munich, Germany), and 5 μL DNA was used. ..

    Gel Extraction:

    Article Title: DNA cleavage assay for the identification of topoisomerase I inhibitors.
    Article Snippet: .. The inhibition of DNA topoisomerase I (Top1) has proven to be a successful approach in the design of anticancer agents. ..

    Article Title: The GLIB technique for genome-wide mapping of 5-hydroxymethylcytosine
    Article Snippet: .. Sodium acetate (Sigma-Aldrich, cat. no. S2889-250G) QIAquick PCR purification kit (Qiagen, cat. no. 28106) QIAquick gel extraction kit (Qiagen, cat. no. 28704) Illustra MicroSpin G-50 columns (GE Healthcare, cat. no. 27-5330-01) Dynabeads M280-Streptavidin (Invitrogen, cat. no. 11206D) QuantIt OliGreen kit (Invitrogen, cat. no. ) PBS, 10× (Meditech CellGro, cat. no. 46-013-CM) Deoxynucleotide solution set (New England Biolabs, cat. no. N0446S) Hydroxymethyl dCTP (Bioline, cat. no. BIO-39046) pUC19 DNA (New England Biolabs, cat. no. N3041S) T4 phage β-glucosyltransferase (T4-BGT; New England Biolabs, cat. no. M0357S) AhdI (New England Biolabs, cat. no. R0584S) FspI (New England Biolabs, cat. no. R0135S) HindIII (New England Biolabs, cat. no. R0104S) NdeI (New England Biolabs, cat. no. R0111S) DNA polymerase, Klenow fragment (New England Biolabs, cat. no. M0210S) Ethanol (Sigma-Aldrich, cat. no. E7023) Tris (Sigma-Aldrich, cat. no. 154563) Agarose (Invitrogen, cat. no. 16500500) ..

    Article Title: Preparation of reduced representation bisulfite sequencing libraries for genome-scale DNA methylation profiling.
    Article Snippet: .. Genome-wide mapping of 5-methylcytosine is of broad interest to many fields of biology and medicine. ..

    Spectrophotometry:

    Article Title: Genetic Screens and Biochemical Assays to Characterize Vibrio cholerae O1 Biotypes: Classical and El Tor
    Article Snippet: .. Overnight Cultures QIAprep Spin Miniprep Kit (Qiagen Cat. #27104) NanoDrop ND100 Spectrophotometer (NanoDrop Model #ND100) 200 μl Sterile Thin-walled PCR Tubes (Corning Cat. #6571) tcpA / ctxB Forward and Reverse Primers 1.25 mM dNTP Mix (NEB Cat. #N0446S) (see recipe) Pfu Turbo Cx HotStart DNA Polymerase (Agilent Cat. #600412-51) 10X Pfu Turbo Cx Reaction Buffer (Stratagene Cat. #600410-52) PCR Thermal Cycler (to hold 200 μl PCR tubes) with Heated Lid UltraPure Agarose (Invitrogen Cat. #16500-500) 1X TBE (see recipe) 6X DNA Gel Loading Dye (see recipe) QIAquick PCR Purification Kit (Qiagen Cat. #28104) Design primers to anneal approximately 50-75 bp upstream and downstream of the translational start and stop sites, respectively, of either tcpA or ctxB . ..

    Purification:

    Article Title: Genetic Screens and Biochemical Assays to Characterize Vibrio cholerae O1 Biotypes: Classical and El Tor
    Article Snippet: .. Overnight Cultures QIAprep Spin Miniprep Kit (Qiagen Cat. #27104) NanoDrop ND100 Spectrophotometer (NanoDrop Model #ND100) 200 μl Sterile Thin-walled PCR Tubes (Corning Cat. #6571) tcpA / ctxB Forward and Reverse Primers 1.25 mM dNTP Mix (NEB Cat. #N0446S) (see recipe) Pfu Turbo Cx HotStart DNA Polymerase (Agilent Cat. #600412-51) 10X Pfu Turbo Cx Reaction Buffer (Stratagene Cat. #600410-52) PCR Thermal Cycler (to hold 200 μl PCR tubes) with Heated Lid UltraPure Agarose (Invitrogen Cat. #16500-500) 1X TBE (see recipe) 6X DNA Gel Loading Dye (see recipe) QIAquick PCR Purification Kit (Qiagen Cat. #28104) Design primers to anneal approximately 50-75 bp upstream and downstream of the translational start and stop sites, respectively, of either tcpA or ctxB . ..

    Article Title: The GLIB technique for genome-wide mapping of 5-hydroxymethylcytosine
    Article Snippet: .. Sodium acetate (Sigma-Aldrich, cat. no. S2889-250G) QIAquick PCR purification kit (Qiagen, cat. no. 28106) QIAquick gel extraction kit (Qiagen, cat. no. 28704) Illustra MicroSpin G-50 columns (GE Healthcare, cat. no. 27-5330-01) Dynabeads M280-Streptavidin (Invitrogen, cat. no. 11206D) QuantIt OliGreen kit (Invitrogen, cat. no. ) PBS, 10× (Meditech CellGro, cat. no. 46-013-CM) Deoxynucleotide solution set (New England Biolabs, cat. no. N0446S) Hydroxymethyl dCTP (Bioline, cat. no. BIO-39046) pUC19 DNA (New England Biolabs, cat. no. N3041S) T4 phage β-glucosyltransferase (T4-BGT; New England Biolabs, cat. no. M0357S) AhdI (New England Biolabs, cat. no. R0584S) FspI (New England Biolabs, cat. no. R0135S) HindIII (New England Biolabs, cat. no. R0104S) NdeI (New England Biolabs, cat. no. R0111S) DNA polymerase, Klenow fragment (New England Biolabs, cat. no. M0210S) Ethanol (Sigma-Aldrich, cat. no. E7023) Tris (Sigma-Aldrich, cat. no. 154563) Agarose (Invitrogen, cat. no. 16500500) ..

    Article Title: Preparation of reduced representation bisulfite sequencing libraries for genome-scale DNA methylation profiling.
    Article Snippet: .. Genome-wide mapping of 5-methylcytosine is of broad interest to many fields of biology and medicine. ..

    Article Title: Assessing Protein Dynamics on Low-Complexity Single-Stranded DNA Curtains
    Article Snippet: .. Low-complexity ssDNA was synthesized in 1× phi29 DNA polymerase reaction buffer (NEB M0269S), 500 μ M dCTP and dTTP (NEB N0446S), 0.2 mg mL−1 BSA (NEB B9000S), 10 nM annealed circles, and 100 nM phi29 DNAP (purified in-house). ..

    Molecular Weight:

    Article Title: Preparation of reduced representation bisulfite sequencing libraries for genome-scale DNA methylation profiling.
    Article Snippet: .. Genome-wide mapping of 5-methylcytosine is of broad interest to many fields of biology and medicine. ..

    SYBR Green Assay:

    Article Title: Preparation of reduced representation bisulfite sequencing libraries for genome-scale DNA methylation profiling.
    Article Snippet: .. Genome-wide mapping of 5-methylcytosine is of broad interest to many fields of biology and medicine. ..

    Staining:

    Article Title: Preparation of reduced representation bisulfite sequencing libraries for genome-scale DNA methylation profiling.
    Article Snippet: .. Genome-wide mapping of 5-methylcytosine is of broad interest to many fields of biology and medicine. ..

    Synthesized:

    Article Title: Assessing Protein Dynamics on Low-Complexity Single-Stranded DNA Curtains
    Article Snippet: .. Low-complexity ssDNA was synthesized in 1× phi29 DNA polymerase reaction buffer (NEB M0269S), 500 μ M dCTP and dTTP (NEB N0446S), 0.2 mg mL−1 BSA (NEB B9000S), 10 nM annealed circles, and 100 nM phi29 DNAP (purified in-house). ..

    other:

    Article Title: Cell-type-specific brain methylomes profiled via ultralow-input microfluidics
    Article Snippet: Single cells were sorted into the 96 well-plate using FACS (BD FACSAria, BD Biosciences).

    Article Title: In situ, Cell-free Protein Expression on Microarrays and Their Use for the Detection of Immune Responses
    Article Snippet: Deoxynucleotide (dNTP) solution set (New England Biolabs, catalog number: N0446S).

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  • Team
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  • 95
    New England Biolabs deoxynucleotide solution set
    Deoxynucleotide Solution Set, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleotide solution set/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    deoxynucleotide solution set - by Bioz Stars, 2021-08
    95/100 stars
      Buy from Supplier

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