ssrna  (New England Biolabs)


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    Name:
    ssRNA Ladder
    Description:
    ssRNA Ladder 25 gel lanes
    Catalog Number:
    n0362s
    Price:
    70
    Size:
    25 gel lanes
    Category:
    RNA Ladders
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    Structured Review

    New England Biolabs ssrna
    ssRNA Ladder
    ssRNA Ladder 25 gel lanes
    https://www.bioz.com/result/ssrna/product/New England Biolabs
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ssrna - by Bioz Stars, 2020-02
    95/100 stars

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    Related Articles

    Amplification:

    Article Title: A novel source for miR-21 expression through the alternative polyadenylation of VMP1 gene transcripts
    Article Snippet: Transcript size was estimated relative to High Range ssRNA Ladder (New England Biolabs). .. Primers and northern blotting probes ( Supplementary Table SI ) were generated from Michigan cancer foundation-7 (MCF-7) cDNA PCR amplified using the GoTaq® qPCR Master Mix (Promega), single bands isolated by QIAquick PCR Purification Kit (Qiagen) and probes labeling by α-32 P-dCTP (Perkin Elmer) and ChromaSpin + TE-10 Columns (Clontech).

    Article Title: Chemotherapy drugs derived nanoparticles encapsulating mRNA encoding tumor suppressor proteins to treat triple-negative breast cancer
    Article Snippet: The resulting plasmid was amplified by PCR to generate the DNA template for transcription. .. P53 mRNA was mixed with equal volume of denaturing loading buffer (8.1 M urea, 0.9 mM EDTA, 0.1% (m/v) xylene cyanol FF, 0.1% (m/v) bromophenol blue in 1×TBE) and ssRNA ladder (NEB) with 4× volume of RNA loading dye (NEB).

    Synthesized:

    Article Title: Fluorescent Single-Stranded DNA Binding Protein as a Probe for Sensitive, Real-Time Assays of Helicase Activity
    Article Snippet: Agarose gel analysis confirmed efficient linearization, and the plasmid substrates were not purified further. ssRNA, as a ladder of seven lengths, and Lambda DNA were obtained from New England Biolabs, and poly(U) and poly(dT) were obtained from Sigma-Aldrich (Gillingham, UK). .. The coumarin labeling reagents, IDCC ( N -[2-(iodoacetamido)ethyl]-7-diethylaminocoumarin-3-carboxamide) and MDCC ( N -[2-(1-maleimidyl)ethyl]-7-diethylaminocoumarin-3-carboxamide), and the single isomer rhodamine iodoacetamide, 6-IATR (6-iodoacetamidotetramethylrhodamine), were synthesized and provided by Dr. J. E. T. Corrie (NIMR, London, UK) ( , ).

    Article Title: Chemotherapy drugs derived nanoparticles encapsulating mRNA encoding tumor suppressor proteins to treat triple-negative breast cancer
    Article Snippet: The uncapped mRNA encoding P53 was synthesized in vitro using AmpliScribe T7-Flash Transcription Kit (Lucigen) and purified with RNA Clean & Concentrator-25 (Zymo), following manufacturers’ instructions. .. P53 mRNA was mixed with equal volume of denaturing loading buffer (8.1 M urea, 0.9 mM EDTA, 0.1% (m/v) xylene cyanol FF, 0.1% (m/v) bromophenol blue in 1×TBE) and ssRNA ladder (NEB) with 4× volume of RNA loading dye (NEB).

    Lambda DNA Preparation:

    Article Title: Fluorescent Single-Stranded DNA Binding Protein as a Probe for Sensitive, Real-Time Assays of Helicase Activity
    Article Snippet: .. Agarose gel analysis confirmed efficient linearization, and the plasmid substrates were not purified further. ssRNA, as a ladder of seven lengths, and Lambda DNA were obtained from New England Biolabs, and poly(U) and poly(dT) were obtained from Sigma-Aldrich (Gillingham, UK). .. The concentrations of these nucleic acids are given in terms of nucleotides. dT35 and dT70 were obtained from Eurogentec, and concentrations are given in terms of moles of oligonucleotide.

    Electrophoresis:

    Article Title: Cauliflower mosaic virus Transcriptome Reveals a Complex Alternative Splicing Pattern
    Article Snippet: 15 μg of RNA were separated by electrophoresis on an agarose (0.8%)-urea (7 M) gel in Tris-acetate-EDTA buffer (40 mM Tris-acetate, 1 mM EDTA). .. An RNA ladder (ssRNA ladder, New England Biolabs) comprising fragments from 0.5 to 9 kb in length was used as a size standard.

    Article Title: Chemotherapy drugs derived nanoparticles encapsulating mRNA encoding tumor suppressor proteins to treat triple-negative breast cancer
    Article Snippet: The length of the final P53 mRNA product was examined by running precast 0.8% agarose E-gel electrophoresis (Invitrogen). .. P53 mRNA was mixed with equal volume of denaturing loading buffer (8.1 M urea, 0.9 mM EDTA, 0.1% (m/v) xylene cyanol FF, 0.1% (m/v) bromophenol blue in 1×TBE) and ssRNA ladder (NEB) with 4× volume of RNA loading dye (NEB).

    Incubation:

    Article Title: Cauliflower mosaic virus Transcriptome Reveals a Complex Alternative Splicing Pattern
    Article Snippet: An RNA ladder (ssRNA ladder, New England Biolabs) comprising fragments from 0.5 to 9 kb in length was used as a size standard. .. The membranes were pre-hybridized in PerfectHyb Plus buffer (Sigma-Aldrich) at 42°C for 15 min and incubated overnight at 42°C with 32 P-radiolabeled DNA oligonucleotide probes (“int”: 5’-CTACAATCTCATTGAGGTTGTTAAGATGA-3’; “ex”: 5’- CTTCCATATTCCGAGTAAGCTTCTTC-3’).

    Article Title: A novel source for miR-21 expression through the alternative polyadenylation of VMP1 gene transcripts
    Article Snippet: Transcript size was estimated relative to High Range ssRNA Ladder (New England Biolabs). .. After an ON incubation in Ultrahyb Oligo Buffer (Ambion), blots were washed in serial dilutions of hot 2 × SSC buffer and 2 × SSC buffer + 0.5% SDS.

    Article Title: Chemotherapy drugs derived nanoparticles encapsulating mRNA encoding tumor suppressor proteins to treat triple-negative breast cancer
    Article Snippet: .. Both P53 mRNA and ssRNA ladder were incubated at 90 °C for 90 s right before gel loading. .. After electrophoresis, image was taken in ChemDoc MP imaging system (Bio-Rad).

    Article Title: Characterization of the RpoN regulon reveals differential regulation of T6SS and new flagellar operons in Vibrio cholerae O37 strain V52
    Article Snippet: .. Northern blot analysis Total RNA (10 μg) and ssRNA ladder (2 μg) were mixed with ssRNA ladder loading buffer (NEB), respectively, and incubated at 75°C for 5 minutes and 4°C for 2 minutes. .. Samples were resolved on a 6% TBE-Urea gel (Life Technologies) at 150 V for 1 hour and transferred to a Hybond N membrane at 70 V for 2 hours.

    Article Title: Engineering circular RNA for potent and stable translation in eukaryotic cells
    Article Snippet: To enrich for circRNA, 20 µg of RNA was diluted in water (86 µL final volume) and then heated at 65 °C for 3 min and cooled on ice for 3 min. 20U RNase R and 10 µL of 10× RNase R buffer (Epicenter) was added, and the reaction was incubated at 37 °C for 15 min; an additional 10U RNase R was added halfway through the reaction. .. RNA was separated on precast 2% E-gel EX agarose gels (Invitrogen) on the E-gel iBase (Invitrogen) using the E-gel EX 1–2% program; ssRNA Ladder (NEB) was used as a standard.

    Article Title: Cloning and Characterization of a Novel Drosophila Stress Induced DNase
    Article Snippet: Analysis of nuclease activity The nuclease activity of the SID fractions was evaluated using the following substrates: double stranded generic plasmid DNA, M13mp18 single stranded DNA (Cat #N4040S; NEB), and single stranded RNA (Cat #N0362S; NEB). .. However, the nuclease activity could be typically visualized following 1–2 hour incubation.

    Article Title: Chemotherapy drugs derived nanoparticles encapsulating mRNA encoding tumor suppressor proteins to treat triple-negative breast cancer
    Article Snippet: P53 mRNA was mixed with equal volume of denaturing loading buffer (8.1 M urea, 0.9 mM EDTA, 0.1% (m/v) xylene cyanol FF, 0.1% (m/v) bromophenol blue in 1×TBE) and ssRNA ladder (NEB) with 4× volume of RNA loading dye (NEB). .. Both P53 mRNA and ssRNA ladder were incubated at 90 °C for 90 s right before gel loading.

    Activity Assay:

    Article Title: Cloning and Characterization of a Novel Drosophila Stress Induced DNase
    Article Snippet: .. Analysis of nuclease activity The nuclease activity of the SID fractions was evaluated using the following substrates: double stranded generic plasmid DNA, M13mp18 single stranded DNA (Cat #N4040S; NEB), and single stranded RNA (Cat #N0362S; NEB). .. A typical 20 µl reaction contained 10 mM Tris-HCl (pH 7.5 unless otherwise indicated), 2.5 mM cation, nucleic acid substrate, and the partially purified protein.

    Western Blot:

    Article Title: Monkeypox virus induces the synthesis of less dsRNA than vaccinia virus, and is more resistant to the anti-poxvirus drug, IBT, than vaccinia virus
    Article Snippet: Western blot analysis was performed with the J2 monoclonal anti-dsRNA antibody (Scicons). .. Probe specificity was verified using dsRNA and ssRNA ladders (New England Biolabs) as positive and negative controls respectively.

    Northern Blot:

    Article Title: Cauliflower mosaic virus Transcriptome Reveals a Complex Alternative Splicing Pattern
    Article Snippet: Paragraph title: Total RNA extraction and northern-blotting ... An RNA ladder (ssRNA ladder, New England Biolabs) comprising fragments from 0.5 to 9 kb in length was used as a size standard.

    Article Title: A novel source for miR-21 expression through the alternative polyadenylation of VMP1 gene transcripts
    Article Snippet: Paragraph title: RNA extraction and northern blotting ... Transcript size was estimated relative to High Range ssRNA Ladder (New England Biolabs).

    Article Title: Control of kidney, eye and limb expression of Bmp7 by an enhancer element highly conserved between species
    Article Snippet: Paragraph title: RNA purification, Northern analysis and RACE ... 50μg total RNA was separated on a 1% formaldehyde denaturing gel alongside an RNA size ladder (ssRNA ladder, New England Biolabs).

    Article Title: Characterization of the RpoN regulon reveals differential regulation of T6SS and new flagellar operons in Vibrio cholerae O37 strain V52
    Article Snippet: .. Northern blot analysis Total RNA (10 μg) and ssRNA ladder (2 μg) were mixed with ssRNA ladder loading buffer (NEB), respectively, and incubated at 75°C for 5 minutes and 4°C for 2 minutes. .. Samples were resolved on a 6% TBE-Urea gel (Life Technologies) at 150 V for 1 hour and transferred to a Hybond N membrane at 70 V for 2 hours.

    Generated:

    Article Title: A novel source for miR-21 expression through the alternative polyadenylation of VMP1 gene transcripts
    Article Snippet: Transcript size was estimated relative to High Range ssRNA Ladder (New England Biolabs). .. Primers and northern blotting probes ( Supplementary Table SI ) were generated from Michigan cancer foundation-7 (MCF-7) cDNA PCR amplified using the GoTaq® qPCR Master Mix (Promega), single bands isolated by QIAquick PCR Purification Kit (Qiagen) and probes labeling by α-32 P-dCTP (Perkin Elmer) and ChromaSpin + TE-10 Columns (Clontech).

    Imaging:

    Article Title: Chemotherapy drugs derived nanoparticles encapsulating mRNA encoding tumor suppressor proteins to treat triple-negative breast cancer
    Article Snippet: P53 mRNA was mixed with equal volume of denaturing loading buffer (8.1 M urea, 0.9 mM EDTA, 0.1% (m/v) xylene cyanol FF, 0.1% (m/v) bromophenol blue in 1×TBE) and ssRNA ladder (NEB) with 4× volume of RNA loading dye (NEB). .. After electrophoresis, image was taken in ChemDoc MP imaging system (Bio-Rad).

    Random Primed:

    Article Title: Control of kidney, eye and limb expression of Bmp7 by an enhancer element highly conserved between species
    Article Snippet: 50μg total RNA was separated on a 1% formaldehyde denaturing gel alongside an RNA size ladder (ssRNA ladder, New England Biolabs). .. The gel was subsequently blotted to Hybond N nitrocellulose (GE Healthcare) ( ) and hybridized with a 32 P-labeled random primed probe (Rediprime, GE Healthcare) representing the entire Bmp7 coding sequence using standard Northern blotting procedures ( ).

    Sequencing:

    Article Title: Control of kidney, eye and limb expression of Bmp7 by an enhancer element highly conserved between species
    Article Snippet: 50μg total RNA was separated on a 1% formaldehyde denaturing gel alongside an RNA size ladder (ssRNA ladder, New England Biolabs). .. The gel was subsequently blotted to Hybond N nitrocellulose (GE Healthcare) ( ) and hybridized with a 32 P-labeled random primed probe (Rediprime, GE Healthcare) representing the entire Bmp7 coding sequence using standard Northern blotting procedures ( ).

    Binding Assay:

    Article Title: Antisense transcription of the myotonic dystrophy locus yields low-abundant RNAs with and without (CAG)n repeat
    Article Snippet: Total RNA was mixed with oligo(dT) latex beads, allowing binding of poly(A) tails of transcripts to the beads, followed by several washing steps using a microfilter and ultimately elution of poly(A)-selected RNA. .. Gel lanes were sliced in multiple fragments, guided by a ssRNA ladder (NEB).

    Nucleic Acid Electrophoresis:

    Article Title: Deoxyribozyme Cascade for Visual Detection of Bacterial RNA
    Article Snippet: Paragraph title: Gel electrophoresis ... To serve as a size marker, ssRNA Ladder (New England BioLabs Inc, MA) (1 μg) was used.

    Article Title: VLPs Derived from the CCMV Plant Virus Can Directly Transfect and Deliver Heterologous Genes for Translation into Mammalian Cells
    Article Snippet: The integrity of mRNA was evaluated by denatured gel electrophoresis with formaldehyde in 1x MOPS as running buffer with diethyl pyrocarbonate (DEPC) water and stained with 0.5 μ l GelRed (Biotium, Fremont CA, USA). .. The mRNA was compared with ssRNA ladder (NEB, Ipswich, MA, USA).

    Mutagenesis:

    Article Title: Fluorescent Single-Stranded DNA Binding Protein as a Probe for Sensitive, Real-Time Assays of Helicase Activity
    Article Snippet: AddAB (AddAD1172A BD961A mutant) and RecBCD were purified as described previously ( , ). .. Agarose gel analysis confirmed efficient linearization, and the plasmid substrates were not purified further. ssRNA, as a ladder of seven lengths, and Lambda DNA were obtained from New England Biolabs, and poly(U) and poly(dT) were obtained from Sigma-Aldrich (Gillingham, UK).

    Isolation:

    Article Title: Antisense transcription of the myotonic dystrophy locus yields low-abundant RNAs with and without (CAG)n repeat
    Article Snippet: Paragraph title: RNA isolation and fractionation ... Gel lanes were sliced in multiple fragments, guided by a ssRNA ladder (NEB).

    Article Title: Deoxyribozyme Cascade for Visual Detection of Bacterial RNA
    Article Snippet: Total bacterial RNA isolated either from M. smegmatis (strain MC2 155) or from M. bovis (strain BCG) (1μg in 5 μL) was mixed with 5 μL of a loading buffer×2 containing 2 mM EDTA, 0.01% bromophenol blue, 7 M urea, 13% Ficoll (w/v), 90 mM Tris-borate, pH 8.3. .. To serve as a size marker, ssRNA Ladder (New England BioLabs Inc, MA) (1 μg) was used.

    Article Title: A novel source for miR-21 expression through the alternative polyadenylation of VMP1 gene transcripts
    Article Snippet: Transcript size was estimated relative to High Range ssRNA Ladder (New England Biolabs). .. Primers and northern blotting probes ( Supplementary Table SI ) were generated from Michigan cancer foundation-7 (MCF-7) cDNA PCR amplified using the GoTaq® qPCR Master Mix (Promega), single bands isolated by QIAquick PCR Purification Kit (Qiagen) and probes labeling by α-32 P-dCTP (Perkin Elmer) and ChromaSpin + TE-10 Columns (Clontech).

    Labeling:

    Article Title: A novel source for miR-21 expression through the alternative polyadenylation of VMP1 gene transcripts
    Article Snippet: Transcript size was estimated relative to High Range ssRNA Ladder (New England Biolabs). .. Primers and northern blotting probes ( Supplementary Table SI ) were generated from Michigan cancer foundation-7 (MCF-7) cDNA PCR amplified using the GoTaq® qPCR Master Mix (Promega), single bands isolated by QIAquick PCR Purification Kit (Qiagen) and probes labeling by α-32 P-dCTP (Perkin Elmer) and ChromaSpin + TE-10 Columns (Clontech).

    Article Title: Characterization of the RpoN regulon reveals differential regulation of T6SS and new flagellar operons in Vibrio cholerae O37 strain V52
    Article Snippet: Northern blot analysis Total RNA (10 μg) and ssRNA ladder (2 μg) were mixed with ssRNA ladder loading buffer (NEB), respectively, and incubated at 75°C for 5 minutes and 4°C for 2 minutes. .. After UV-cross-linking twice at 1200 J, the membrane was pre-hybridized with ULTRAhyb®-Oligo buffer (Life Technologies) at 45°C for 30 minutes and then hybridized with 100 pmol of 32 P labeled probe overnight.

    Article Title: Fluorescent Single-Stranded DNA Binding Protein as a Probe for Sensitive, Real-Time Assays of Helicase Activity
    Article Snippet: Agarose gel analysis confirmed efficient linearization, and the plasmid substrates were not purified further. ssRNA, as a ladder of seven lengths, and Lambda DNA were obtained from New England Biolabs, and poly(U) and poly(dT) were obtained from Sigma-Aldrich (Gillingham, UK). .. The coumarin labeling reagents, IDCC ( N -[2-(iodoacetamido)ethyl]-7-diethylaminocoumarin-3-carboxamide) and MDCC ( N -[2-(1-maleimidyl)ethyl]-7-diethylaminocoumarin-3-carboxamide), and the single isomer rhodamine iodoacetamide, 6-IATR (6-iodoacetamidotetramethylrhodamine), were synthesized and provided by Dr. J. E. T. Corrie (NIMR, London, UK) ( , ).

    Purification:

    Article Title: A novel source for miR-21 expression through the alternative polyadenylation of VMP1 gene transcripts
    Article Snippet: Transcript size was estimated relative to High Range ssRNA Ladder (New England Biolabs). .. Primers and northern blotting probes ( Supplementary Table SI ) were generated from Michigan cancer foundation-7 (MCF-7) cDNA PCR amplified using the GoTaq® qPCR Master Mix (Promega), single bands isolated by QIAquick PCR Purification Kit (Qiagen) and probes labeling by α-32 P-dCTP (Perkin Elmer) and ChromaSpin + TE-10 Columns (Clontech).

    Article Title: Control of kidney, eye and limb expression of Bmp7 by an enhancer element highly conserved between species
    Article Snippet: Paragraph title: RNA purification, Northern analysis and RACE ... 50μg total RNA was separated on a 1% formaldehyde denaturing gel alongside an RNA size ladder (ssRNA ladder, New England Biolabs).

    Article Title: Engineering circular RNA for potent and stable translation in eukaryotic cells
    Article Snippet: Paragraph title: circRNA design and purification ... RNA was separated on precast 2% E-gel EX agarose gels (Invitrogen) on the E-gel iBase (Invitrogen) using the E-gel EX 1–2% program; ssRNA Ladder (NEB) was used as a standard.

    Article Title: Fluorescent Single-Stranded DNA Binding Protein as a Probe for Sensitive, Real-Time Assays of Helicase Activity
    Article Snippet: .. Agarose gel analysis confirmed efficient linearization, and the plasmid substrates were not purified further. ssRNA, as a ladder of seven lengths, and Lambda DNA were obtained from New England Biolabs, and poly(U) and poly(dT) were obtained from Sigma-Aldrich (Gillingham, UK). .. The concentrations of these nucleic acids are given in terms of nucleotides. dT35 and dT70 were obtained from Eurogentec, and concentrations are given in terms of moles of oligonucleotide.

    Article Title: VLPs Derived from the CCMV Plant Virus Can Directly Transfect and Deliver Heterologous Genes for Translation into Mammalian Cells
    Article Snippet: RNA transcript was treated with RQ1 RNase-free DNase (1 U/μ g) for 15 min at 37°C followed by a phenol : chloroform purification protocol and eluted in nuclease-free water. .. The mRNA was compared with ssRNA ladder (NEB, Ipswich, MA, USA).

    Article Title: Cloning and Characterization of a Novel Drosophila Stress Induced DNase
    Article Snippet: Analysis of nuclease activity The nuclease activity of the SID fractions was evaluated using the following substrates: double stranded generic plasmid DNA, M13mp18 single stranded DNA (Cat #N4040S; NEB), and single stranded RNA (Cat #N0362S; NEB). .. A typical 20 µl reaction contained 10 mM Tris-HCl (pH 7.5 unless otherwise indicated), 2.5 mM cation, nucleic acid substrate, and the partially purified protein.

    Article Title: Chemotherapy drugs derived nanoparticles encapsulating mRNA encoding tumor suppressor proteins to treat triple-negative breast cancer
    Article Snippet: The uncapped mRNA encoding P53 was synthesized in vitro using AmpliScribe T7-Flash Transcription Kit (Lucigen) and purified with RNA Clean & Concentrator-25 (Zymo), following manufacturers’ instructions. .. P53 mRNA was mixed with equal volume of denaturing loading buffer (8.1 M urea, 0.9 mM EDTA, 0.1% (m/v) xylene cyanol FF, 0.1% (m/v) bromophenol blue in 1×TBE) and ssRNA ladder (NEB) with 4× volume of RNA loading dye (NEB).

    Dot Blot:

    Article Title: Monkeypox virus induces the synthesis of less dsRNA than vaccinia virus, and is more resistant to the anti-poxvirus drug, IBT, than vaccinia virus
    Article Snippet: Paragraph title: Slot Blot for dsRNA ... Probe specificity was verified using dsRNA and ssRNA ladders (New England Biolabs) as positive and negative controls respectively.

    Polymerase Chain Reaction:

    Article Title: A novel source for miR-21 expression through the alternative polyadenylation of VMP1 gene transcripts
    Article Snippet: Transcript size was estimated relative to High Range ssRNA Ladder (New England Biolabs). .. Primers and northern blotting probes ( Supplementary Table SI ) were generated from Michigan cancer foundation-7 (MCF-7) cDNA PCR amplified using the GoTaq® qPCR Master Mix (Promega), single bands isolated by QIAquick PCR Purification Kit (Qiagen) and probes labeling by α-32 P-dCTP (Perkin Elmer) and ChromaSpin + TE-10 Columns (Clontech).

    Article Title: Chemotherapy drugs derived nanoparticles encapsulating mRNA encoding tumor suppressor proteins to treat triple-negative breast cancer
    Article Snippet: The resulting plasmid was amplified by PCR to generate the DNA template for transcription. .. P53 mRNA was mixed with equal volume of denaturing loading buffer (8.1 M urea, 0.9 mM EDTA, 0.1% (m/v) xylene cyanol FF, 0.1% (m/v) bromophenol blue in 1×TBE) and ssRNA ladder (NEB) with 4× volume of RNA loading dye (NEB).

    Lysis:

    Article Title: Antisense transcription of the myotonic dystrophy locus yields low-abundant RNAs with and without (CAG)n repeat
    Article Snippet: The supernatant (cytoplasmic fraction) and the pellet (nuclear fraction) were mixed with lysis buffer according to instructions of the Aurum Total RNA Mini Kit (Bio-Rad) and RNA was isolated. .. Gel lanes were sliced in multiple fragments, guided by a ssRNA ladder (NEB).

    Plasmid Preparation:

    Article Title: Fluorescent Single-Stranded DNA Binding Protein as a Probe for Sensitive, Real-Time Assays of Helicase Activity
    Article Snippet: .. Agarose gel analysis confirmed efficient linearization, and the plasmid substrates were not purified further. ssRNA, as a ladder of seven lengths, and Lambda DNA were obtained from New England Biolabs, and poly(U) and poly(dT) were obtained from Sigma-Aldrich (Gillingham, UK). .. The concentrations of these nucleic acids are given in terms of nucleotides. dT35 and dT70 were obtained from Eurogentec, and concentrations are given in terms of moles of oligonucleotide.

    Article Title: Cloning and Characterization of a Novel Drosophila Stress Induced DNase
    Article Snippet: .. Analysis of nuclease activity The nuclease activity of the SID fractions was evaluated using the following substrates: double stranded generic plasmid DNA, M13mp18 single stranded DNA (Cat #N4040S; NEB), and single stranded RNA (Cat #N0362S; NEB). .. A typical 20 µl reaction contained 10 mM Tris-HCl (pH 7.5 unless otherwise indicated), 2.5 mM cation, nucleic acid substrate, and the partially purified protein.

    Article Title: Chemotherapy drugs derived nanoparticles encapsulating mRNA encoding tumor suppressor proteins to treat triple-negative breast cancer
    Article Snippet: The resulting plasmid was amplified by PCR to generate the DNA template for transcription. .. P53 mRNA was mixed with equal volume of denaturing loading buffer (8.1 M urea, 0.9 mM EDTA, 0.1% (m/v) xylene cyanol FF, 0.1% (m/v) bromophenol blue in 1×TBE) and ssRNA ladder (NEB) with 4× volume of RNA loading dye (NEB).

    Real-time Polymerase Chain Reaction:

    Article Title: A novel source for miR-21 expression through the alternative polyadenylation of VMP1 gene transcripts
    Article Snippet: Transcript size was estimated relative to High Range ssRNA Ladder (New England Biolabs). .. Primers and northern blotting probes ( Supplementary Table SI ) were generated from Michigan cancer foundation-7 (MCF-7) cDNA PCR amplified using the GoTaq® qPCR Master Mix (Promega), single bands isolated by QIAquick PCR Purification Kit (Qiagen) and probes labeling by α-32 P-dCTP (Perkin Elmer) and ChromaSpin + TE-10 Columns (Clontech).

    RNA Extraction:

    Article Title: Cauliflower mosaic virus Transcriptome Reveals a Complex Alternative Splicing Pattern
    Article Snippet: Paragraph title: Total RNA extraction and northern-blotting ... An RNA ladder (ssRNA ladder, New England Biolabs) comprising fragments from 0.5 to 9 kb in length was used as a size standard.

    Article Title: A novel source for miR-21 expression through the alternative polyadenylation of VMP1 gene transcripts
    Article Snippet: Paragraph title: RNA extraction and northern blotting ... Transcript size was estimated relative to High Range ssRNA Ladder (New England Biolabs).

    Agarose Gel Electrophoresis:

    Article Title: Antisense transcription of the myotonic dystrophy locus yields low-abundant RNAs with and without (CAG)n repeat
    Article Snippet: For size-based RNA fractionation, total RNA, denatured in 50% formamide at 70°C for 10 min, was electrophoresed in a 1.2% (w/v) MOPS-buffered agarose gel stained with ethidium bromide. .. Gel lanes were sliced in multiple fragments, guided by a ssRNA ladder (NEB).

    Article Title: Engineering circular RNA for potent and stable translation in eukaryotic cells
    Article Snippet: RNA was separated on precast 2% E-gel EX agarose gels (Invitrogen) on the E-gel iBase (Invitrogen) using the E-gel EX 1–2% program; ssRNA Ladder (NEB) was used as a standard. .. We were unable to obtain adequate circRNA separation using other agarose gel systems.

    Article Title: Fluorescent Single-Stranded DNA Binding Protein as a Probe for Sensitive, Real-Time Assays of Helicase Activity
    Article Snippet: .. Agarose gel analysis confirmed efficient linearization, and the plasmid substrates were not purified further. ssRNA, as a ladder of seven lengths, and Lambda DNA were obtained from New England Biolabs, and poly(U) and poly(dT) were obtained from Sigma-Aldrich (Gillingham, UK). .. The concentrations of these nucleic acids are given in terms of nucleotides. dT35 and dT70 were obtained from Eurogentec, and concentrations are given in terms of moles of oligonucleotide.

    Article Title: VLPs Derived from the CCMV Plant Virus Can Directly Transfect and Deliver Heterologous Genes for Translation into Mammalian Cells
    Article Snippet: The in vitro transcripts were denatured for 15 min at 65°C and then kept on ice for 5 min before they were loaded onto 1.2% agarose gel. .. The mRNA was compared with ssRNA ladder (NEB, Ipswich, MA, USA).

    In Vitro:

    Article Title: VLPs Derived from the CCMV Plant Virus Can Directly Transfect and Deliver Heterologous Genes for Translation into Mammalian Cells
    Article Snippet: The in vitro transcripts were denatured for 15 min at 65°C and then kept on ice for 5 min before they were loaded onto 1.2% agarose gel. .. The mRNA was compared with ssRNA ladder (NEB, Ipswich, MA, USA).

    Article Title: Chemotherapy drugs derived nanoparticles encapsulating mRNA encoding tumor suppressor proteins to treat triple-negative breast cancer
    Article Snippet: The uncapped mRNA encoding P53 was synthesized in vitro using AmpliScribe T7-Flash Transcription Kit (Lucigen) and purified with RNA Clean & Concentrator-25 (Zymo), following manufacturers’ instructions. .. P53 mRNA was mixed with equal volume of denaturing loading buffer (8.1 M urea, 0.9 mM EDTA, 0.1% (m/v) xylene cyanol FF, 0.1% (m/v) bromophenol blue in 1×TBE) and ssRNA ladder (NEB) with 4× volume of RNA loading dye (NEB).

    Spectrophotometry:

    Article Title: VLPs Derived from the CCMV Plant Virus Can Directly Transfect and Deliver Heterologous Genes for Translation into Mammalian Cells
    Article Snippet: The mRNA was compared with ssRNA ladder (NEB, Ipswich, MA, USA). .. The purity was measured using the UV-Vis spectrophotometer (NanoDrop 2000C, ThermoScientific, MA, USA) and gave values for all samples of at least 2.0 and 2.3 for these ratios, respectively, indicating a very good purity.

    Concentration Assay:

    Article Title: Engineering circular RNA for potent and stable translation in eukaryotic cells
    Article Snippet: In some cases, purified RNA was recircularized: RNA was heated to 70 °C for 5 min and then immediately placed on ice for 3 min, after which GTP was added to a final concentration of 2 mM along with a buffer including magnesium (50 mM Tris-HCl, 10 mM MgCl2, 1 mM DTT, pH 7.5; New England Biolabs). .. RNA was separated on precast 2% E-gel EX agarose gels (Invitrogen) on the E-gel iBase (Invitrogen) using the E-gel EX 1–2% program; ssRNA Ladder (NEB) was used as a standard.

    Article Title: Chemotherapy drugs derived nanoparticles encapsulating mRNA encoding tumor suppressor proteins to treat triple-negative breast cancer
    Article Snippet: After determination of concentration by Nanodrop (Thermo), the mRNA was stored in 1×TE at −80 °C until use. .. P53 mRNA was mixed with equal volume of denaturing loading buffer (8.1 M urea, 0.9 mM EDTA, 0.1% (m/v) xylene cyanol FF, 0.1% (m/v) bromophenol blue in 1×TBE) and ssRNA ladder (NEB) with 4× volume of RNA loading dye (NEB).

    Fractionation:

    Article Title: Antisense transcription of the myotonic dystrophy locus yields low-abundant RNAs with and without (CAG)n repeat
    Article Snippet: Paragraph title: RNA isolation and fractionation ... Gel lanes were sliced in multiple fragments, guided by a ssRNA ladder (NEB).

    Migration:

    Article Title: Control of kidney, eye and limb expression of Bmp7 by an enhancer element highly conserved between species
    Article Snippet: 50μg total RNA was separated on a 1% formaldehyde denaturing gel alongside an RNA size ladder (ssRNA ladder, New England Biolabs). .. The gel was ethidium bromide stained and nucleic acid migration distances calculated.

    Marker:

    Article Title: Deoxyribozyme Cascade for Visual Detection of Bacterial RNA
    Article Snippet: .. To serve as a size marker, ssRNA Ladder (New England BioLabs Inc, MA) (1 μg) was used. ..

    Staining:

    Article Title: Antisense transcription of the myotonic dystrophy locus yields low-abundant RNAs with and without (CAG)n repeat
    Article Snippet: For size-based RNA fractionation, total RNA, denatured in 50% formamide at 70°C for 10 min, was electrophoresed in a 1.2% (w/v) MOPS-buffered agarose gel stained with ethidium bromide. .. Gel lanes were sliced in multiple fragments, guided by a ssRNA ladder (NEB).

    Article Title: Deoxyribozyme Cascade for Visual Detection of Bacterial RNA
    Article Snippet: To serve as a size marker, ssRNA Ladder (New England BioLabs Inc, MA) (1 μg) was used. .. The gel was run at 100 V for 1 h, stained with GelRed (Biotium, CA), and the bands were visualized using a U:Genius gel documentation system (Syngene, MD).

    Article Title: Control of kidney, eye and limb expression of Bmp7 by an enhancer element highly conserved between species
    Article Snippet: 50μg total RNA was separated on a 1% formaldehyde denaturing gel alongside an RNA size ladder (ssRNA ladder, New England Biolabs). .. The gel was ethidium bromide stained and nucleic acid migration distances calculated.

    Article Title: VLPs Derived from the CCMV Plant Virus Can Directly Transfect and Deliver Heterologous Genes for Translation into Mammalian Cells
    Article Snippet: The integrity of mRNA was evaluated by denatured gel electrophoresis with formaldehyde in 1x MOPS as running buffer with diethyl pyrocarbonate (DEPC) water and stained with 0.5 μ l GelRed (Biotium, Fremont CA, USA). .. The mRNA was compared with ssRNA ladder (NEB, Ipswich, MA, USA).

    Gradient Centrifugation:

    Article Title: Fluorescent Single-Stranded DNA Binding Protein as a Probe for Sensitive, Real-Time Assays of Helicase Activity
    Article Snippet: The pBR322 and pADF0 plasmids were purified using Maxiprep kits (Qiagen, Valencia, CA) and cesium chloride density gradient centrifugation, and then linearized with the Eco RI or ClaI restriction endonucleases. .. Agarose gel analysis confirmed efficient linearization, and the plasmid substrates were not purified further. ssRNA, as a ladder of seven lengths, and Lambda DNA were obtained from New England Biolabs, and poly(U) and poly(dT) were obtained from Sigma-Aldrich (Gillingham, UK).

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    New England Biolabs ssrna ladder
    <t>mRNA-EGFP</t> transcription. Capped (lane 3) and uncapped (lane 4) mRNAs that code for EGFP were synthesized along with a luciferase mRNA (control, lane 2). Their integrity was visually evaluated using electrophoresis agarose gels stained with GelRed™. Transcripts are compared with the <t>ssRNA</t> ladder (lane 1). Single gel experiment without cropping.
    Ssrna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mRNA-EGFP transcription. Capped (lane 3) and uncapped (lane 4) mRNAs that code for EGFP were synthesized along with a luciferase mRNA (control, lane 2). Their integrity was visually evaluated using electrophoresis agarose gels stained with GelRed™. Transcripts are compared with the ssRNA ladder (lane 1). Single gel experiment without cropping.

    Journal: BioMed Research International

    Article Title: VLPs Derived from the CCMV Plant Virus Can Directly Transfect and Deliver Heterologous Genes for Translation into Mammalian Cells

    doi: 10.1155/2019/4630891

    Figure Lengend Snippet: mRNA-EGFP transcription. Capped (lane 3) and uncapped (lane 4) mRNAs that code for EGFP were synthesized along with a luciferase mRNA (control, lane 2). Their integrity was visually evaluated using electrophoresis agarose gels stained with GelRed™. Transcripts are compared with the ssRNA ladder (lane 1). Single gel experiment without cropping.

    Article Snippet: The mRNA was compared with ssRNA ladder (NEB, Ipswich, MA, USA).

    Techniques: Synthesized, Luciferase, Electrophoresis, Staining