range ssrna ladder  (New England Biolabs)


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    Name:
    ssRNA Ladder
    Description:
    ssRNA Ladder 25 gel lanes
    Catalog Number:
    n0362s
    Price:
    70
    Size:
    25 gel lanes
    Category:
    RNA Ladders
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    Structured Review

    New England Biolabs range ssrna ladder
    ssRNA Ladder
    ssRNA Ladder 25 gel lanes
    https://www.bioz.com/result/range ssrna ladder/product/New England Biolabs
    Average 97 stars, based on 41 article reviews
    Price from $9.99 to $1999.99
    range ssrna ladder - by Bioz Stars, 2020-09
    97/100 stars

    Images

    1) Product Images from "Co-transcriptional folding of a bio-orthogonal fluorescent scaffolded RNA origami"

    Article Title: Co-transcriptional folding of a bio-orthogonal fluorescent scaffolded RNA origami

    Journal: bioRxiv

    doi: 10.1101/864678

    In-gel imaging of co-transcriptional folded light-up RNA origami. 10% TBE gel electrophoresis after DFHBI-1T (a) and after SYBR® Gold (b) staining. The gel was stained with DFHBI-1T for 25 min to visualize Broccoli aptamer (positive control) and co-transcriptional folded RNA origami. After 3 washing steps, the gel was stained with SYBR® Gold for 5 min to detect transcribed RNA. Lanes: 1: low range ssRNA ladder; 2: Broccoli aptamer; 3: transcribed RNA scaffold; 4: transcribed RNA staples; 5: transcribed scaffold, s1 and s2 staples; 6: transcribed scaffold, s1, s2, l1 and r1 staples; 7: transcribed RNA origami. Molecular size in nucleotides are indicated.
    Figure Legend Snippet: In-gel imaging of co-transcriptional folded light-up RNA origami. 10% TBE gel electrophoresis after DFHBI-1T (a) and after SYBR® Gold (b) staining. The gel was stained with DFHBI-1T for 25 min to visualize Broccoli aptamer (positive control) and co-transcriptional folded RNA origami. After 3 washing steps, the gel was stained with SYBR® Gold for 5 min to detect transcribed RNA. Lanes: 1: low range ssRNA ladder; 2: Broccoli aptamer; 3: transcribed RNA scaffold; 4: transcribed RNA staples; 5: transcribed scaffold, s1 and s2 staples; 6: transcribed scaffold, s1, s2, l1 and r1 staples; 7: transcribed RNA origami. Molecular size in nucleotides are indicated.

    Techniques Used: Imaging, Nucleic Acid Electrophoresis, Staining, Positive Control

    6% TBE gel electrophoresis of transcription products after SYBR® Gold staining. Lanes. 1: transcribed RNA scaffold; 2: transcribed RNA staple strands; 3: transcribed RNA scaffold, Staples s1 and s2; 4: transcribed RNA scaffold and RNA Staples s1, s2, r1 and l1; 5: co-transcriptional folded RNA (black arrow); 6: low range ssRNA ladder. Molecular size in nucleotides are indicated.
    Figure Legend Snippet: 6% TBE gel electrophoresis of transcription products after SYBR® Gold staining. Lanes. 1: transcribed RNA scaffold; 2: transcribed RNA staple strands; 3: transcribed RNA scaffold, Staples s1 and s2; 4: transcribed RNA scaffold and RNA Staples s1, s2, r1 and l1; 5: co-transcriptional folded RNA (black arrow); 6: low range ssRNA ladder. Molecular size in nucleotides are indicated.

    Techniques Used: Nucleic Acid Electrophoresis, Staining

    2) Product Images from "VLPs Derived from the CCMV Plant Virus Can Directly Transfect and Deliver Heterologous Genes for Translation into Mammalian Cells"

    Article Title: VLPs Derived from the CCMV Plant Virus Can Directly Transfect and Deliver Heterologous Genes for Translation into Mammalian Cells

    Journal: BioMed Research International

    doi: 10.1155/2019/4630891

    mRNA-EGFP transcription. Capped (lane 3) and uncapped (lane 4) mRNAs that code for EGFP were synthesized along with a luciferase mRNA (control, lane 2). Their integrity was visually evaluated using electrophoresis agarose gels stained with GelRed™. Transcripts are compared with the ssRNA ladder (lane 1). Single gel experiment without cropping.
    Figure Legend Snippet: mRNA-EGFP transcription. Capped (lane 3) and uncapped (lane 4) mRNAs that code for EGFP were synthesized along with a luciferase mRNA (control, lane 2). Their integrity was visually evaluated using electrophoresis agarose gels stained with GelRed™. Transcripts are compared with the ssRNA ladder (lane 1). Single gel experiment without cropping.

    Techniques Used: Synthesized, Luciferase, Electrophoresis, Staining

    3) Product Images from "The Zinc-Fingers of KREPA3 Are Essential for the Complete Editing of Mitochondrial mRNAs in Trypanosoma brucei"

    Article Title: The Zinc-Fingers of KREPA3 Are Essential for the Complete Editing of Mitochondrial mRNAs in Trypanosoma brucei

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0008913

    Loss of KREPA3 or mutation of KREPA3 ZF2 has no effect on gRNA levels. Total cellular RNA from KREPA3-RKO, RKO-KREPA3 WT-myc and ZFm2-myc cells with KREP3 Reg expressed (E) or repressed (R) were examined by a capping assay using guanylyltransferse and [α- 32 P]GTP. The similarly labeled ssRNA ladder was used to size the gRNAs which show their characteristics size heterogeneity due to their variable oligo-U tails.
    Figure Legend Snippet: Loss of KREPA3 or mutation of KREPA3 ZF2 has no effect on gRNA levels. Total cellular RNA from KREPA3-RKO, RKO-KREPA3 WT-myc and ZFm2-myc cells with KREP3 Reg expressed (E) or repressed (R) were examined by a capping assay using guanylyltransferse and [α- 32 P]GTP. The similarly labeled ssRNA ladder was used to size the gRNAs which show their characteristics size heterogeneity due to their variable oligo-U tails.

    Techniques Used: Mutagenesis, Labeling

    4) Product Images from "Biochemical analysis of the Cas6-1 RNA endonuclease associated with the subtype I-D CRISPR-Cas system in Synechocystis sp. PCC 6803"

    Article Title: Biochemical analysis of the Cas6-1 RNA endonuclease associated with the subtype I-D CRISPR-Cas system in Synechocystis sp. PCC 6803

    Journal: RNA Biology

    doi: 10.1080/15476286.2018.1447742

    Purification and in vitro cleavage assay of Synechocystis WT Cas6-1 and mutated Cas6-1 proteins. (A) Coomassie-stained 15% SDS-PAGE of heterologously overexpressed and purified Cas6-1 protein variants. The expected molecular weight of Cas6-1_6xHis is ∼ 30 kDa. 66.67 pmol recombinant enzyme was loaded on each lane and PageRuler™ Prestained Protein Ladder (Thermo Fisher Scientific) served for protein size estimation. (B, C) In in vitro cleavage assays both the (B) CRISPR1 RNA oligo repeat sequence and the (C) pre-acrRNA cassette is processed with variable efficiency by the purified Cas6-1 protein variants shown in (A). After incubation at 30°C the RNA fragments were electrophoretically size separated on a 15% PAA sequencing gel and visualized with SYBR® Gold Nucleic Acid Gel Stain. The processing results in a pattern of RNA fragments of distinct lengths. Cleavage assays were conducted with a three-fold excess (B) or a 1:1 ratio (C) of protein to RNA-substrate in cleavage buffer and incubated at 30°C for the indicated time. Expected RNA fragments and their corresponding sizes are illustrated by cartoons. Another Cas6-1 WT protein sample, which was overexpressed and purified independently, is indicated by an asterisk. Negative controls were complemented with 1 μL of H 2 O or 1 μL eluate of a mock control. Two additional nucleotides resulting from in vitro transcription were added to the 215 nt long acrRNA yielding a 217 nt long pre-acrRNA. For RNA size estimation a CRISPR1 repeat oligo ladder (C1L), RiboRuler low range RNA ladder (Thermo Fisher Scientific), low range ssRNA ladder or microRNA marker (both from New England Biolabs) were used.
    Figure Legend Snippet: Purification and in vitro cleavage assay of Synechocystis WT Cas6-1 and mutated Cas6-1 proteins. (A) Coomassie-stained 15% SDS-PAGE of heterologously overexpressed and purified Cas6-1 protein variants. The expected molecular weight of Cas6-1_6xHis is ∼ 30 kDa. 66.67 pmol recombinant enzyme was loaded on each lane and PageRuler™ Prestained Protein Ladder (Thermo Fisher Scientific) served for protein size estimation. (B, C) In in vitro cleavage assays both the (B) CRISPR1 RNA oligo repeat sequence and the (C) pre-acrRNA cassette is processed with variable efficiency by the purified Cas6-1 protein variants shown in (A). After incubation at 30°C the RNA fragments were electrophoretically size separated on a 15% PAA sequencing gel and visualized with SYBR® Gold Nucleic Acid Gel Stain. The processing results in a pattern of RNA fragments of distinct lengths. Cleavage assays were conducted with a three-fold excess (B) or a 1:1 ratio (C) of protein to RNA-substrate in cleavage buffer and incubated at 30°C for the indicated time. Expected RNA fragments and their corresponding sizes are illustrated by cartoons. Another Cas6-1 WT protein sample, which was overexpressed and purified independently, is indicated by an asterisk. Negative controls were complemented with 1 μL of H 2 O or 1 μL eluate of a mock control. Two additional nucleotides resulting from in vitro transcription were added to the 215 nt long acrRNA yielding a 217 nt long pre-acrRNA. For RNA size estimation a CRISPR1 repeat oligo ladder (C1L), RiboRuler low range RNA ladder (Thermo Fisher Scientific), low range ssRNA ladder or microRNA marker (both from New England Biolabs) were used.

    Techniques Used: Purification, In Vitro, Cleavage Assay, Staining, SDS Page, Molecular Weight, Recombinant, Sequencing, Incubation, Marker

    Related Articles

    Marker:

    Article Title: Biochemical analysis of the Cas6-1 RNA endonuclease associated with the subtype I-D CRISPR-Cas system in Synechocystis sp. PCC 6803
    Article Snippet: .. The low range ssRNA ladder (ssRNA marker) (New England Biolabs), the RiboRuler low range RNA ladder (Thermo Fisher Scientific) and the microRNA marker (New England Biolabs) served as RNA ladders for size estimation. .. The CRISPR1 repeat oligo ladder (C1L) was generated by alkaline hydrolysis (50 mM Tris-HCl pH 8.5, 20 mM MgCl2 ) with 25 pmol substrate and was incubated for 72 h to 96 h at 30°C.

    Article Title: Co-transcriptional folding of a bio-orthogonal fluorescent scaffolded RNA origami
    Article Snippet: .. The low range ssRNA ladder (NEB) and ZR small-RNA™ ladder (Cambridge Bioscience) were used as molecular weight marker. .. 2.7 Co-transcriptional folding of RNA origami nanoribbon The concentrations of each dsDNA template encoding the RNA staples strands and scaffold were measured using NanoDrop One/OneC spectrophotometer (average concentration values were calculated from 3 measurements for each sample).

    Article Title: Isothermal folding of a light-up bio-orthogonal RNA origami nanoribbon
    Article Snippet: .. The low range ssRNA ladder (NEB) was used as molecular weight marker. .. The set of staple strands (see Supplementary Table ) were mixed in 10-fold excess with RNA scaffold in 50 μL of folding buffer (10 mM MgCl2 , 20 mM Tris-HCl pH 7.6, 1 mM EDTA pH 8.0) .

    Northern Blot:

    Article Title: Global mapping transcriptional start sites revealed both transcriptional and post-transcriptional regulation of cold adaptation in the methanogenic archaeon Methanolobus psychrophilus
    Article Snippet: .. Northern blot analysis 2–5 μg total RNA per lane was denatured for 10 min at 65°C in the loading buffer containing 95% (v/v) formamide and separated on 8% polyacrylamide gels containing 7.6 M urea with a low range ssRNA ladder (New England Biolabs). .. After separation, RNAs were transferred onto Hybond-N+ membranes (GE Healthcare) by electroblotting and cross-linked to the membrane using UV.

    Labeling:

    Article Title: The Zinc-Fingers of KREPA3 Are Essential for the Complete Editing of Mitochondrial mRNAs in Trypanosoma brucei
    Article Snippet: .. Labeled gRNAs were identified by size in comparison to the labeled low range ssRNA ladder (NEB). ..

    Molecular Weight:

    Article Title: Co-transcriptional folding of a bio-orthogonal fluorescent scaffolded RNA origami
    Article Snippet: .. The low range ssRNA ladder (NEB) and ZR small-RNA™ ladder (Cambridge Bioscience) were used as molecular weight marker. .. 2.7 Co-transcriptional folding of RNA origami nanoribbon The concentrations of each dsDNA template encoding the RNA staples strands and scaffold were measured using NanoDrop One/OneC spectrophotometer (average concentration values were calculated from 3 measurements for each sample).

    Article Title: Isothermal folding of a light-up bio-orthogonal RNA origami nanoribbon
    Article Snippet: .. The low range ssRNA ladder (NEB) was used as molecular weight marker. .. The set of staple strands (see Supplementary Table ) were mixed in 10-fold excess with RNA scaffold in 50 μL of folding buffer (10 mM MgCl2 , 20 mM Tris-HCl pH 7.6, 1 mM EDTA pH 8.0) .

    Article Title: An antiviral self-replicating molecular heterotroph
    Article Snippet: .. Trackit Ultra low range DNA ladder (Invitrogen), Low Molecular Weight DNA Ladder (New England Biolabs), ssRNA ladder and Low range ssRNA ladder standards (New England Biolabs) were used. .. In specific cases, samples were also run on Novex 10% Tris borate-EDTA (TBE) polyacrylamide gel (Invitrogen) and 10% mini-PROTEAN TBE-Urea Precast gels (Bio-Rad).

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  • 97
    New England Biolabs range ssrna ladder
    In-gel imaging of co-transcriptional folded light-up <t>RNA</t> origami. 10% TBE gel electrophoresis after DFHBI-1T (a) and after SYBR® Gold (b) staining. The gel was stained with DFHBI-1T for 25 min to visualize Broccoli aptamer (positive control) and co-transcriptional folded RNA origami. After 3 washing steps, the gel was stained with SYBR® Gold for 5 min to detect transcribed RNA. Lanes: 1: low range <t>ssRNA</t> ladder; 2: Broccoli aptamer; 3: transcribed RNA scaffold; 4: transcribed RNA staples; 5: transcribed scaffold, s1 and s2 staples; 6: transcribed scaffold, s1, s2, l1 and r1 staples; 7: transcribed RNA origami. Molecular size in nucleotides are indicated.
    Range Ssrna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/range ssrna ladder/product/New England Biolabs
    Average 97 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    range ssrna ladder - by Bioz Stars, 2020-09
    97/100 stars
      Buy from Supplier

    Image Search Results


    In-gel imaging of co-transcriptional folded light-up RNA origami. 10% TBE gel electrophoresis after DFHBI-1T (a) and after SYBR® Gold (b) staining. The gel was stained with DFHBI-1T for 25 min to visualize Broccoli aptamer (positive control) and co-transcriptional folded RNA origami. After 3 washing steps, the gel was stained with SYBR® Gold for 5 min to detect transcribed RNA. Lanes: 1: low range ssRNA ladder; 2: Broccoli aptamer; 3: transcribed RNA scaffold; 4: transcribed RNA staples; 5: transcribed scaffold, s1 and s2 staples; 6: transcribed scaffold, s1, s2, l1 and r1 staples; 7: transcribed RNA origami. Molecular size in nucleotides are indicated.

    Journal: bioRxiv

    Article Title: Co-transcriptional folding of a bio-orthogonal fluorescent scaffolded RNA origami

    doi: 10.1101/864678

    Figure Lengend Snippet: In-gel imaging of co-transcriptional folded light-up RNA origami. 10% TBE gel electrophoresis after DFHBI-1T (a) and after SYBR® Gold (b) staining. The gel was stained with DFHBI-1T for 25 min to visualize Broccoli aptamer (positive control) and co-transcriptional folded RNA origami. After 3 washing steps, the gel was stained with SYBR® Gold for 5 min to detect transcribed RNA. Lanes: 1: low range ssRNA ladder; 2: Broccoli aptamer; 3: transcribed RNA scaffold; 4: transcribed RNA staples; 5: transcribed scaffold, s1 and s2 staples; 6: transcribed scaffold, s1, s2, l1 and r1 staples; 7: transcribed RNA origami. Molecular size in nucleotides are indicated.

    Article Snippet: The low range ssRNA ladder (NEB) and ZR small-RNA™ ladder (Cambridge Bioscience) were used as molecular weight marker.

    Techniques: Imaging, Nucleic Acid Electrophoresis, Staining, Positive Control

    6% TBE gel electrophoresis of transcription products after SYBR® Gold staining. Lanes. 1: transcribed RNA scaffold; 2: transcribed RNA staple strands; 3: transcribed RNA scaffold, Staples s1 and s2; 4: transcribed RNA scaffold and RNA Staples s1, s2, r1 and l1; 5: co-transcriptional folded RNA (black arrow); 6: low range ssRNA ladder. Molecular size in nucleotides are indicated.

    Journal: bioRxiv

    Article Title: Co-transcriptional folding of a bio-orthogonal fluorescent scaffolded RNA origami

    doi: 10.1101/864678

    Figure Lengend Snippet: 6% TBE gel electrophoresis of transcription products after SYBR® Gold staining. Lanes. 1: transcribed RNA scaffold; 2: transcribed RNA staple strands; 3: transcribed RNA scaffold, Staples s1 and s2; 4: transcribed RNA scaffold and RNA Staples s1, s2, r1 and l1; 5: co-transcriptional folded RNA (black arrow); 6: low range ssRNA ladder. Molecular size in nucleotides are indicated.

    Article Snippet: The low range ssRNA ladder (NEB) and ZR small-RNA™ ladder (Cambridge Bioscience) were used as molecular weight marker.

    Techniques: Nucleic Acid Electrophoresis, Staining

    mRNA-EGFP transcription. Capped (lane 3) and uncapped (lane 4) mRNAs that code for EGFP were synthesized along with a luciferase mRNA (control, lane 2). Their integrity was visually evaluated using electrophoresis agarose gels stained with GelRed™. Transcripts are compared with the ssRNA ladder (lane 1). Single gel experiment without cropping.

    Journal: BioMed Research International

    Article Title: VLPs Derived from the CCMV Plant Virus Can Directly Transfect and Deliver Heterologous Genes for Translation into Mammalian Cells

    doi: 10.1155/2019/4630891

    Figure Lengend Snippet: mRNA-EGFP transcription. Capped (lane 3) and uncapped (lane 4) mRNAs that code for EGFP were synthesized along with a luciferase mRNA (control, lane 2). Their integrity was visually evaluated using electrophoresis agarose gels stained with GelRed™. Transcripts are compared with the ssRNA ladder (lane 1). Single gel experiment without cropping.

    Article Snippet: The mRNA was compared with ssRNA ladder (NEB, Ipswich, MA, USA).

    Techniques: Synthesized, Luciferase, Electrophoresis, Staining

    Loss of KREPA3 or mutation of KREPA3 ZF2 has no effect on gRNA levels. Total cellular RNA from KREPA3-RKO, RKO-KREPA3 WT-myc and ZFm2-myc cells with KREP3 Reg expressed (E) or repressed (R) were examined by a capping assay using guanylyltransferse and [α- 32 P]GTP. The similarly labeled ssRNA ladder was used to size the gRNAs which show their characteristics size heterogeneity due to their variable oligo-U tails.

    Journal: PLoS ONE

    Article Title: The Zinc-Fingers of KREPA3 Are Essential for the Complete Editing of Mitochondrial mRNAs in Trypanosoma brucei

    doi: 10.1371/journal.pone.0008913

    Figure Lengend Snippet: Loss of KREPA3 or mutation of KREPA3 ZF2 has no effect on gRNA levels. Total cellular RNA from KREPA3-RKO, RKO-KREPA3 WT-myc and ZFm2-myc cells with KREP3 Reg expressed (E) or repressed (R) were examined by a capping assay using guanylyltransferse and [α- 32 P]GTP. The similarly labeled ssRNA ladder was used to size the gRNAs which show their characteristics size heterogeneity due to their variable oligo-U tails.

    Article Snippet: Labeled gRNAs were identified by size in comparison to the labeled low range ssRNA ladder (NEB).

    Techniques: Mutagenesis, Labeling

    Purification and in vitro cleavage assay of Synechocystis WT Cas6-1 and mutated Cas6-1 proteins. (A) Coomassie-stained 15% SDS-PAGE of heterologously overexpressed and purified Cas6-1 protein variants. The expected molecular weight of Cas6-1_6xHis is ∼ 30 kDa. 66.67 pmol recombinant enzyme was loaded on each lane and PageRuler™ Prestained Protein Ladder (Thermo Fisher Scientific) served for protein size estimation. (B, C) In in vitro cleavage assays both the (B) CRISPR1 RNA oligo repeat sequence and the (C) pre-acrRNA cassette is processed with variable efficiency by the purified Cas6-1 protein variants shown in (A). After incubation at 30°C the RNA fragments were electrophoretically size separated on a 15% PAA sequencing gel and visualized with SYBR® Gold Nucleic Acid Gel Stain. The processing results in a pattern of RNA fragments of distinct lengths. Cleavage assays were conducted with a three-fold excess (B) or a 1:1 ratio (C) of protein to RNA-substrate in cleavage buffer and incubated at 30°C for the indicated time. Expected RNA fragments and their corresponding sizes are illustrated by cartoons. Another Cas6-1 WT protein sample, which was overexpressed and purified independently, is indicated by an asterisk. Negative controls were complemented with 1 μL of H 2 O or 1 μL eluate of a mock control. Two additional nucleotides resulting from in vitro transcription were added to the 215 nt long acrRNA yielding a 217 nt long pre-acrRNA. For RNA size estimation a CRISPR1 repeat oligo ladder (C1L), RiboRuler low range RNA ladder (Thermo Fisher Scientific), low range ssRNA ladder or microRNA marker (both from New England Biolabs) were used.

    Journal: RNA Biology

    Article Title: Biochemical analysis of the Cas6-1 RNA endonuclease associated with the subtype I-D CRISPR-Cas system in Synechocystis sp. PCC 6803

    doi: 10.1080/15476286.2018.1447742

    Figure Lengend Snippet: Purification and in vitro cleavage assay of Synechocystis WT Cas6-1 and mutated Cas6-1 proteins. (A) Coomassie-stained 15% SDS-PAGE of heterologously overexpressed and purified Cas6-1 protein variants. The expected molecular weight of Cas6-1_6xHis is ∼ 30 kDa. 66.67 pmol recombinant enzyme was loaded on each lane and PageRuler™ Prestained Protein Ladder (Thermo Fisher Scientific) served for protein size estimation. (B, C) In in vitro cleavage assays both the (B) CRISPR1 RNA oligo repeat sequence and the (C) pre-acrRNA cassette is processed with variable efficiency by the purified Cas6-1 protein variants shown in (A). After incubation at 30°C the RNA fragments were electrophoretically size separated on a 15% PAA sequencing gel and visualized with SYBR® Gold Nucleic Acid Gel Stain. The processing results in a pattern of RNA fragments of distinct lengths. Cleavage assays were conducted with a three-fold excess (B) or a 1:1 ratio (C) of protein to RNA-substrate in cleavage buffer and incubated at 30°C for the indicated time. Expected RNA fragments and their corresponding sizes are illustrated by cartoons. Another Cas6-1 WT protein sample, which was overexpressed and purified independently, is indicated by an asterisk. Negative controls were complemented with 1 μL of H 2 O or 1 μL eluate of a mock control. Two additional nucleotides resulting from in vitro transcription were added to the 215 nt long acrRNA yielding a 217 nt long pre-acrRNA. For RNA size estimation a CRISPR1 repeat oligo ladder (C1L), RiboRuler low range RNA ladder (Thermo Fisher Scientific), low range ssRNA ladder or microRNA marker (both from New England Biolabs) were used.

    Article Snippet: The low range ssRNA ladder (ssRNA marker) (New England Biolabs), the RiboRuler low range RNA ladder (Thermo Fisher Scientific) and the microRNA marker (New England Biolabs) served as RNA ladders for size estimation.

    Techniques: Purification, In Vitro, Cleavage Assay, Staining, SDS Page, Molecular Weight, Recombinant, Sequencing, Incubation, Marker