midrange pfg marker  (New England Biolabs)


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    Name:
    MidRange PFG Marker
    Description:
    MidRange PFG Marker 50 gel lanes
    Catalog Number:
    n0342s
    Price:
    156
    Size:
    50 gel lanes
    Category:
    DNA Ladders
    Buy from Supplier


    Structured Review

    New England Biolabs midrange pfg marker
    MidRange PFG Marker
    MidRange PFG Marker 50 gel lanes
    https://www.bioz.com/result/midrange pfg marker/product/New England Biolabs
    Average 94 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    midrange pfg marker - by Bioz Stars, 2020-07
    94/100 stars

    Images

    1) Product Images from "Evaluation of Two Approaches for Assessing the Genetic Similarity of Virioplankton Populations as Defined by Genome Size"

    Article Title: Evaluation of Two Approaches for Assessing the Genetic Similarity of Virioplankton Populations as Defined by Genome Size

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.02432-12

    Pulsed-field gels of virioplankton concentrates. Gels are labeled according to the month and year of sample collection. Lanes are labeled by station designation in the Chesapeake Bay (CB) and Delaware Bay (DB). Samples from the June 2006 and July 2007 cruises were loaded at two different concentrations of virus particles: 10 9 (A) and ∼10 10 (B). DNA was purified from bands marked with numbers. Marker lanes (in kilobases) are as follows: M1, concatemers of phage λ genome mixed with HindIII digest of λ genomic DNA; M2, concatemers of phage λ genome; M3, λ DNA digested with HindIII; M4, midrange PFG marker.
    Figure Legend Snippet: Pulsed-field gels of virioplankton concentrates. Gels are labeled according to the month and year of sample collection. Lanes are labeled by station designation in the Chesapeake Bay (CB) and Delaware Bay (DB). Samples from the June 2006 and July 2007 cruises were loaded at two different concentrations of virus particles: 10 9 (A) and ∼10 10 (B). DNA was purified from bands marked with numbers. Marker lanes (in kilobases) are as follows: M1, concatemers of phage λ genome mixed with HindIII digest of λ genomic DNA; M2, concatemers of phage λ genome; M3, λ DNA digested with HindIII; M4, midrange PFG marker.

    Techniques Used: Labeling, Purification, Marker

    2) Product Images from "Giant panda BAC library construction and assembly of a 650-kb contig spanning major histocompatibility complex class II region"

    Article Title: Giant panda BAC library construction and assembly of a 650-kb contig spanning major histocompatibility complex class II region

    Journal: BMC Genomics

    doi: 10.1186/1471-2164-8-315

    (a) Insert size analysis of randomly selected BAC clones. There are 14 BAC clones tested. The BAC DNA was completely digested with Not I enzyme and determined by pulsed field gel electrophoresis with 1–20 second switch time, 6 V/cm at 12°C for 16 hours. The MidRange II PFG markers (New England BioLabs) were used as size standard in the right flanking lane. The size of pCC1BAC vector is 8.1 kb. (b) Distribution of insert size in the giant panda BAC library. Insert sizes were evaluated by PFGE after Not I restriction enzyme digestion from 174 BAC clones.
    Figure Legend Snippet: (a) Insert size analysis of randomly selected BAC clones. There are 14 BAC clones tested. The BAC DNA was completely digested with Not I enzyme and determined by pulsed field gel electrophoresis with 1–20 second switch time, 6 V/cm at 12°C for 16 hours. The MidRange II PFG markers (New England BioLabs) were used as size standard in the right flanking lane. The size of pCC1BAC vector is 8.1 kb. (b) Distribution of insert size in the giant panda BAC library. Insert sizes were evaluated by PFGE after Not I restriction enzyme digestion from 174 BAC clones.

    Techniques Used: BAC Assay, Clone Assay, Pulsed-Field Gel, Electrophoresis, Plasmid Preparation

    Related Articles

    Marker:

    Article Title: A Baculovirus alkaline nuclease knockout construct produces fragmented DNA and aberrant capsids
    Article Snippet: .. MidRange PFG marker I and Yeast chromosome PFG markers (New England Biolabs) were used as DNA size markers. .. For Southern blot analysis, the agarose gel was soaked in 0.25M HCl for 15 min followed by 0.4M NaOH for 15 min.

    Article Title: Cloning, Nucleotide Sequencing, and Functional Analysis of a Novel, Mobile Cluster of Biodegradation Genes from Pseudomonas aeruginosa Strain JB2
    Article Snippet: .. Plasmids were separated by using a CHEF-DR II (Bio-Rad Laboratories, Richmond, Calif.) run at 200 V (21 h, 14°C) with switching times ramping from 1 to 40 s. The mid-range PFG Marker II (New England Biolabs, Beverly, Mass.) was used as a molecular weight marker for linear DNA. ..

    Article Title: Evaluation of Two Approaches for Assessing the Genetic Similarity of Virioplankton Populations as Defined by Genome Size
    Article Snippet: .. Concatemers of phage lambda genomic DNA (Bio-Rad), HindIII digest of phage lambda genomic DNA (Fisher Scientific), and MidRange PFG marker (New England BioLabs) were used as molecular weight markers. .. Gels were stained in a 1:10,000 dilution of SYBR Gold (Invitrogen) for 30 min and imaged on a Typhoon 9400 variable mode imager (GE Healthcare).

    Article Title: Whole genome sequencing and comparative genomic analyses of two Vibrio cholerae O139 Bengal-specific Podoviruses to other N4-like phages reveal extensive genetic diversity
    Article Snippet: .. Pulsed-field gel electrophoresis was done using a CHEF-DR-III apparatus (BioRad, Hercules, CA) under the following conditions: Gels (1%) were cast using Megabase agarose (BioRad) and run at 14°C buffer temperature with 1-25 s switch time, 6 V/cm and 120° angle for 17.5 h. PFGE MidRange Marker I and II (New England Biolabs, Ipswich, MA) were used for estimating fragment sizes. .. Primers for genome-end runoff sequencing were designed based on the consensus genome sequence and Sanger sequencing reactions were carried out using either intact ϕJA1 DNA or isolated restriction fragments as templates.

    Article Title: Characterization and Chromosomal Mapping of Antimicrobial Resistance Genes in Salmonella enterica Serotype Typhimurium
    Article Snippet: .. Electrophoresis was performed at 200 V using a Gene Navigator system with a hexagonal electrode array (Pharmacia LKB Biotechnology AB, Uppsala, Sweden) in the interpolation mode pulsing from 1 through 40 s. Molecular weight markers included mid-range PFG Markers (New England BioLabs, Hertfordshire, United Kingdom), and digoxigenin-labeled DNA molecular weight marker grade II (Roche Diagnostics, Lewes, East Sussex, United Kingdom). .. Gels were stained with 0.5 mg of ethidium bromide per liter in distilled water before being photographed over a UV transilluminator.

    Article Title: Giant panda BAC library construction and assembly of a 650-kb contig spanning major histocompatibility complex class II region
    Article Snippet: .. Mid-range PFG marker (New England Biolabs) was used as DNA size marker. .. The gel was stained with ethidium bromide and photographed.

    Electrophoresis:

    Article Title: Characterization and Chromosomal Mapping of Antimicrobial Resistance Genes in Salmonella enterica Serotype Typhimurium
    Article Snippet: .. Electrophoresis was performed at 200 V using a Gene Navigator system with a hexagonal electrode array (Pharmacia LKB Biotechnology AB, Uppsala, Sweden) in the interpolation mode pulsing from 1 through 40 s. Molecular weight markers included mid-range PFG Markers (New England BioLabs, Hertfordshire, United Kingdom), and digoxigenin-labeled DNA molecular weight marker grade II (Roche Diagnostics, Lewes, East Sussex, United Kingdom). .. Gels were stained with 0.5 mg of ethidium bromide per liter in distilled water before being photographed over a UV transilluminator.

    Size-exclusion Chromatography:

    Article Title: Organization of the cpe Locus in CPE-Positive Clostridium perfringens Type C and D Isolates
    Article Snippet: .. The running parameters were: initial pulse, 1 sec; final pulse, 25 sec; voltage, 6 V/cm, 24 h. Mid-Range PFGE markers (New England Biolabs) were used as molecular size markers. .. After PFGE, the gel was stained with ethidium bromide, washed with distilled water, and photographed.

    Molecular Weight:

    Article Title: Cloning, Nucleotide Sequencing, and Functional Analysis of a Novel, Mobile Cluster of Biodegradation Genes from Pseudomonas aeruginosa Strain JB2
    Article Snippet: .. Plasmids were separated by using a CHEF-DR II (Bio-Rad Laboratories, Richmond, Calif.) run at 200 V (21 h, 14°C) with switching times ramping from 1 to 40 s. The mid-range PFG Marker II (New England Biolabs, Beverly, Mass.) was used as a molecular weight marker for linear DNA. ..

    Article Title: Evaluation of Two Approaches for Assessing the Genetic Similarity of Virioplankton Populations as Defined by Genome Size
    Article Snippet: .. Concatemers of phage lambda genomic DNA (Bio-Rad), HindIII digest of phage lambda genomic DNA (Fisher Scientific), and MidRange PFG marker (New England BioLabs) were used as molecular weight markers. .. Gels were stained in a 1:10,000 dilution of SYBR Gold (Invitrogen) for 30 min and imaged on a Typhoon 9400 variable mode imager (GE Healthcare).

    Article Title: Characterization and Chromosomal Mapping of Antimicrobial Resistance Genes in Salmonella enterica Serotype Typhimurium
    Article Snippet: .. Electrophoresis was performed at 200 V using a Gene Navigator system with a hexagonal electrode array (Pharmacia LKB Biotechnology AB, Uppsala, Sweden) in the interpolation mode pulsing from 1 through 40 s. Molecular weight markers included mid-range PFG Markers (New England BioLabs, Hertfordshire, United Kingdom), and digoxigenin-labeled DNA molecular weight marker grade II (Roche Diagnostics, Lewes, East Sussex, United Kingdom). .. Gels were stained with 0.5 mg of ethidium bromide per liter in distilled water before being photographed over a UV transilluminator.

    Pulsed-Field Gel:

    Article Title: Whole genome sequencing and comparative genomic analyses of two Vibrio cholerae O139 Bengal-specific Podoviruses to other N4-like phages reveal extensive genetic diversity
    Article Snippet: .. Pulsed-field gel electrophoresis was done using a CHEF-DR-III apparatus (BioRad, Hercules, CA) under the following conditions: Gels (1%) were cast using Megabase agarose (BioRad) and run at 14°C buffer temperature with 1-25 s switch time, 6 V/cm and 120° angle for 17.5 h. PFGE MidRange Marker I and II (New England Biolabs, Ipswich, MA) were used for estimating fragment sizes. .. Primers for genome-end runoff sequencing were designed based on the consensus genome sequence and Sanger sequencing reactions were carried out using either intact ϕJA1 DNA or isolated restriction fragments as templates.

    Similar Products

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  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    New England Biolabs midrange pfg marker
    Pulsed-field gels of virioplankton concentrates. Gels are labeled according to the month and year of sample collection. Lanes are labeled by station designation in the Chesapeake Bay (CB) and Delaware Bay (DB). Samples from the June 2006 and July 2007 cruises were loaded at two different concentrations of virus particles: 10 9 (A) and ∼10 10 (B). <t>DNA</t> was purified from bands marked with numbers. Marker lanes (in kilobases) are as follows: M1, concatemers of phage λ genome mixed with HindIII digest of λ genomic DNA; M2, concatemers of phage λ genome; M3, λ DNA digested with HindIII; M4, midrange <t>PFG</t> marker.
    Midrange Pfg Marker, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/midrange pfg marker/product/New England Biolabs
    Average 94 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    midrange pfg marker - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    Image Search Results


    Pulsed-field gels of virioplankton concentrates. Gels are labeled according to the month and year of sample collection. Lanes are labeled by station designation in the Chesapeake Bay (CB) and Delaware Bay (DB). Samples from the June 2006 and July 2007 cruises were loaded at two different concentrations of virus particles: 10 9 (A) and ∼10 10 (B). DNA was purified from bands marked with numbers. Marker lanes (in kilobases) are as follows: M1, concatemers of phage λ genome mixed with HindIII digest of λ genomic DNA; M2, concatemers of phage λ genome; M3, λ DNA digested with HindIII; M4, midrange PFG marker.

    Journal: Applied and Environmental Microbiology

    Article Title: Evaluation of Two Approaches for Assessing the Genetic Similarity of Virioplankton Populations as Defined by Genome Size

    doi: 10.1128/AEM.02432-12

    Figure Lengend Snippet: Pulsed-field gels of virioplankton concentrates. Gels are labeled according to the month and year of sample collection. Lanes are labeled by station designation in the Chesapeake Bay (CB) and Delaware Bay (DB). Samples from the June 2006 and July 2007 cruises were loaded at two different concentrations of virus particles: 10 9 (A) and ∼10 10 (B). DNA was purified from bands marked with numbers. Marker lanes (in kilobases) are as follows: M1, concatemers of phage λ genome mixed with HindIII digest of λ genomic DNA; M2, concatemers of phage λ genome; M3, λ DNA digested with HindIII; M4, midrange PFG marker.

    Article Snippet: Concatemers of phage lambda genomic DNA (Bio-Rad), HindIII digest of phage lambda genomic DNA (Fisher Scientific), and MidRange PFG marker (New England BioLabs) were used as molecular weight markers.

    Techniques: Labeling, Purification, Marker

    (a) Insert size analysis of randomly selected BAC clones. There are 14 BAC clones tested. The BAC DNA was completely digested with Not I enzyme and determined by pulsed field gel electrophoresis with 1–20 second switch time, 6 V/cm at 12°C for 16 hours. The MidRange II PFG markers (New England BioLabs) were used as size standard in the right flanking lane. The size of pCC1BAC vector is 8.1 kb. (b) Distribution of insert size in the giant panda BAC library. Insert sizes were evaluated by PFGE after Not I restriction enzyme digestion from 174 BAC clones.

    Journal: BMC Genomics

    Article Title: Giant panda BAC library construction and assembly of a 650-kb contig spanning major histocompatibility complex class II region

    doi: 10.1186/1471-2164-8-315

    Figure Lengend Snippet: (a) Insert size analysis of randomly selected BAC clones. There are 14 BAC clones tested. The BAC DNA was completely digested with Not I enzyme and determined by pulsed field gel electrophoresis with 1–20 second switch time, 6 V/cm at 12°C for 16 hours. The MidRange II PFG markers (New England BioLabs) were used as size standard in the right flanking lane. The size of pCC1BAC vector is 8.1 kb. (b) Distribution of insert size in the giant panda BAC library. Insert sizes were evaluated by PFGE after Not I restriction enzyme digestion from 174 BAC clones.

    Article Snippet: Mid-range PFG marker (New England Biolabs) was used as DNA size marker.

    Techniques: BAC Assay, Clone Assay, Pulsed-Field Gel, Electrophoresis, Plasmid Preparation