midrange pfg marker  (New England Biolabs)


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    Name:
    MidRange PFG Marker
    Description:
    MidRange PFG Marker 50 gel lanes
    Catalog Number:
    n0342s
    Price:
    153
    Size:
    50 gel lanes
    Category:
    DNA Ladders
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    Structured Review

    New England Biolabs midrange pfg marker
    MidRange PFG Marker
    MidRange PFG Marker 50 gel lanes
    https://www.bioz.com/result/midrange pfg marker/product/New England Biolabs
    Average 90 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    midrange pfg marker - by Bioz Stars, 2020-01
    90/100 stars

    Images

    1) Product Images from "Evaluation of Two Approaches for Assessing the Genetic Similarity of Virioplankton Populations as Defined by Genome Size"

    Article Title: Evaluation of Two Approaches for Assessing the Genetic Similarity of Virioplankton Populations as Defined by Genome Size

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.02432-12

    Pulsed-field gels of virioplankton concentrates. Gels are labeled according to the month and year of sample collection. Lanes are labeled by station designation in the Chesapeake Bay (CB) and Delaware Bay (DB). Samples from the June 2006 and July 2007 cruises were loaded at two different concentrations of virus particles: 10 9 (A) and ∼10 10 (B). DNA was purified from bands marked with numbers. Marker lanes (in kilobases) are as follows: M1, concatemers of phage λ genome mixed with HindIII digest of λ genomic DNA; M2, concatemers of phage λ genome; M3, λ DNA digested with HindIII; M4, midrange PFG marker.
    Figure Legend Snippet: Pulsed-field gels of virioplankton concentrates. Gels are labeled according to the month and year of sample collection. Lanes are labeled by station designation in the Chesapeake Bay (CB) and Delaware Bay (DB). Samples from the June 2006 and July 2007 cruises were loaded at two different concentrations of virus particles: 10 9 (A) and ∼10 10 (B). DNA was purified from bands marked with numbers. Marker lanes (in kilobases) are as follows: M1, concatemers of phage λ genome mixed with HindIII digest of λ genomic DNA; M2, concatemers of phage λ genome; M3, λ DNA digested with HindIII; M4, midrange PFG marker.

    Techniques Used: Labeling, Purification, Marker

    Related Articles

    Clone Assay:

    Article Title: Purification and partial genome characterization of the bacterial endosymbiont Blattabacterium cuenoti from the fat bodies of cockroaches
    Article Snippet: M2: MidRange PFG Marker I (New England Biolabs). .. Click here for file Additional file 2 Table S1 Sequencing analysis of the shotgun library clones .

    Article Title: Dynamic Rearrangements Determine Genome Organization and Useful Traits in Soybean 1Dynamic Rearrangements Determine Genome Organization and Useful Traits in Soybean 1 [C]Dynamic Rearrangements Determine Genome Organization and Useful Traits in Soybean 1 [C] [W]
    Article Snippet: Final screening was carried out with candidate BAC clones obtained directly from a working copy of the library. .. Electrophoresis was performed using a 1% agarose gel in Tris-acetate EDTA buffer for 14 h at 6 V cm−1 with an initial pulse time of 5 s and a final pulse time of 15 s. MidRange PFG Marker I (New England Biolabs) was used as a fragment size marker.

    Centrifugation:

    Article Title: Intercalation of small molecules into DNA in chromatin is primarily controlled by superhelical constraint
    Article Snippet: Fragment size was identified using a pulse marker (Midrange PFG marker New England Biolabs N0342S) that was run alongside the samples. .. Nuclei was recovered by centrifugation at 6,500g for 5 min and then washed in extraction buffer without detergent.

    Positive Control:

    Article Title: Dynamic Rearrangements Determine Genome Organization and Useful Traits in Soybean 1Dynamic Rearrangements Determine Genome Organization and Useful Traits in Soybean 1 [C]Dynamic Rearrangements Determine Genome Organization and Useful Traits in Soybean 1 [C] [W]
    Article Snippet: During the entire screening procedure, Williams 82 was used as a positive control. .. Electrophoresis was performed using a 1% agarose gel in Tris-acetate EDTA buffer for 14 h at 6 V cm−1 with an initial pulse time of 5 s and a final pulse time of 15 s. MidRange PFG Marker I (New England Biolabs) was used as a fragment size marker.

    End-sequence Profiling:

    Article Title: Characterization and Chromosomal Mapping of Antimicrobial Resistance Genes in Salmonella enterica Serotype Typhimurium
    Article Snippet: Plugs were washed three times in ESP solution (0.5 M EDTA [pH 8], 10% [wt/vol] lauroylsarcosine, and proteinase K [Sigma] at a final concentration of 100 mg/liter). .. Electrophoresis was performed at 200 V using a Gene Navigator system with a hexagonal electrode array (Pharmacia LKB Biotechnology AB, Uppsala, Sweden) in the interpolation mode pulsing from 1 through 40 s. Molecular weight markers included mid-range PFG Markers (New England BioLabs, Hertfordshire, United Kingdom), and digoxigenin-labeled DNA molecular weight marker grade II (Roche Diagnostics, Lewes, East Sussex, United Kingdom).

    SYBR Green Assay:

    Article Title: Isolation and Characterization of Bacteriophages Infecting the Fish Pathogen Flavobacterium psychrophilum
    Article Snippet: After electrophoresis, the DNA was visualized by staining with Sybr green I in 0.5× Tris-borate-EDTA. .. The PFGE size standards used were an 8- to 48-kb standard (Bio-Rad) and MidRange PFGE Marker I (New England Biolabs).

    Incubation:

    Article Title: Intercalation of small molecules into DNA in chromatin is primarily controlled by superhelical constraint
    Article Snippet: Fragment size was identified using a pulse marker (Midrange PFG marker New England Biolabs N0342S) that was run alongside the samples. .. Briefly, 107 HeLa cells were resuspended in 1 ml of extraction buffer (10 mM HEPES, pH 7.7, 10 mM KCl, 1.5 mM MgCl2 , 0.34 M sucrose, 10% glycerol, 20x dilution protease inhibitor cocktail and 0.2% NP-40) and incubated on ice for 10 min.

    Article Title: A Baculovirus alkaline nuclease knockout construct produces fragmented DNA and aberrant capsids
    Article Snippet: For DNA digestion, the proteinase-treated plugs were incubated in 100 μl of Eco 81I reaction buffer containing 30U Eco 81I and 20U Dpn I (both from Fermantas) and incubated at 37ºC overnight. .. MidRange PFG marker I and Yeast chromosome PFG markers (New England Biolabs) were used as DNA size markers.

    Article Title: Human Immunodeficiency Virus Replication in a Primary Effusion Lymphoma Cell Line Stimulates Lytic-Phase Replication of Kaposi's Sarcoma-Associated Herpesvirus
    Article Snippet: Plugs were incubated for 12 to 18 h at 50°C in proteinase K reaction buffer (100 mM EDTA [pH 8.0], 0.2% sodium deoxycholate, 1% sodium lauryl sarcosine, 1 mg of proteinase K per ml), washed four times in wash buffer (20 mM Tris [pH 8.0], 50 mM EDTA], and stored at 4°C until the assay was performed. .. Two different DNA size markers, which included Lambda ladder PFG and mid-range PFG markers (New England Bio labs, Beverly, Mass.) were run on either side of the samples for precise size determination of the linear DNA.

    Western Blot:

    Article Title: Intercalation of small molecules into DNA in chromatin is primarily controlled by superhelical constraint
    Article Snippet: Paragraph title: Supporting information Representative images and fluorescence profile of EBr stained cells/nuclei. Biphasic dependence of EBr intercalation on salt. Effect of salt treatment on nuclear size. Formaldehyde crosslinking significantly reduces EBr inte rcalation in RNA depleted, salt treated nuclei, relative to ethanol fixation. Raw image for gel blot in : Nick frequency as a function of x-ray irradiation dose. ... Gel was stained with 0.5 μg/ml ethidium bromide and imaged using FluorChem Q (Alpha Innotech, San Leandro, California, USA) gel documentation system; λ-Lambda DNA, L; Molecular Weight Marker (Midrange PFG marker New England Biolabs N0342S).

    Hybridization:

    Article Title: A Baculovirus alkaline nuclease knockout construct produces fragmented DNA and aberrant capsids
    Article Snippet: Paragraph title: DNA transfection, viral infection, field inversion gel electrophoresis and hybridization ... MidRange PFG marker I and Yeast chromosome PFG markers (New England Biolabs) were used as DNA size markers.

    Transfection:

    Article Title: A Baculovirus alkaline nuclease knockout construct produces fragmented DNA and aberrant capsids
    Article Snippet: Paragraph title: DNA transfection, viral infection, field inversion gel electrophoresis and hybridization ... MidRange PFG marker I and Yeast chromosome PFG markers (New England Biolabs) were used as DNA size markers.

    Southern Blot:

    Article Title: A Baculovirus alkaline nuclease knockout construct produces fragmented DNA and aberrant capsids
    Article Snippet: MidRange PFG marker I and Yeast chromosome PFG markers (New England Biolabs) were used as DNA size markers. .. For Southern blot analysis, the agarose gel was soaked in 0.25M HCl for 15 min followed by 0.4M NaOH for 15 min.

    Protease Inhibitor:

    Article Title: Intercalation of small molecules into DNA in chromatin is primarily controlled by superhelical constraint
    Article Snippet: Fragment size was identified using a pulse marker (Midrange PFG marker New England Biolabs N0342S) that was run alongside the samples. .. Briefly, 107 HeLa cells were resuspended in 1 ml of extraction buffer (10 mM HEPES, pH 7.7, 10 mM KCl, 1.5 mM MgCl2 , 0.34 M sucrose, 10% glycerol, 20x dilution protease inhibitor cocktail and 0.2% NP-40) and incubated on ice for 10 min.

    Infection:

    Article Title: A Baculovirus alkaline nuclease knockout construct produces fragmented DNA and aberrant capsids
    Article Snippet: Paragraph title: DNA transfection, viral infection, field inversion gel electrophoresis and hybridization ... MidRange PFG marker I and Yeast chromosome PFG markers (New England Biolabs) were used as DNA size markers.

    Article Title: The temperate Burkholderia phage AP3 of the Peduovirinae shows efficient antimicrobial activity against B. cenocepacia of the IIIA lineage
    Article Snippet: Pulsed-field gel electrophoresis (PFGE) was employed to discriminate the clonal origin of B. cenocepacia IIIA isolates sensitive to AP3 infection. .. A linear ramp of 5 to 65 s was used, and gels were run for 20 h at 200 V. MidRange PFGE Marker (New England Biolabs) was used as a mass marker.

    Article Title: Human Immunodeficiency Virus Replication in a Primary Effusion Lymphoma Cell Line Stimulates Lytic-Phase Replication of Kaposi's Sarcoma-Associated Herpesvirus
    Article Snippet: At 48 h post-TPA treatment or post-HIV infection, the cells were harvested and suspended in cell suspension buffer (10 mM Tris [pH 7.2], 20 mM NaCl, 50 mM EDTA). .. Two different DNA size markers, which included Lambda ladder PFG and mid-range PFG markers (New England Bio labs, Beverly, Mass.) were run on either side of the samples for precise size determination of the linear DNA.

    Sequencing:

    Article Title: Whole genome sequencing and comparative genomic analyses of two Vibrio cholerae O139 Bengal-specific Podoviruses to other N4-like phages reveal extensive genetic diversity
    Article Snippet: Pulsed-field gel electrophoresis was done using a CHEF-DR-III apparatus (BioRad, Hercules, CA) under the following conditions: Gels (1%) were cast using Megabase agarose (BioRad) and run at 14°C buffer temperature with 1-25 s switch time, 6 V/cm and 120° angle for 17.5 h. PFGE MidRange Marker I and II (New England Biolabs, Ipswich, MA) were used for estimating fragment sizes. .. Primers for genome-end runoff sequencing were designed based on the consensus genome sequence and Sanger sequencing reactions were carried out using either intact ϕJA1 DNA or isolated restriction fragments as templates.

    Article Title: Purification and partial genome characterization of the bacterial endosymbiont Blattabacterium cuenoti from the fat bodies of cockroaches
    Article Snippet: M2: MidRange PFG Marker I (New England Biolabs). .. Click here for file Additional file 2 Table S1 Sequencing analysis of the shotgun library clones .

    Article Title: Dynamic Rearrangements Determine Genome Organization and Useful Traits in Soybean 1Dynamic Rearrangements Determine Genome Organization and Useful Traits in Soybean 1 [C]Dynamic Rearrangements Determine Genome Organization and Useful Traits in Soybean 1 [C] [W]
    Article Snippet: Paragraph title: Identification, Sequencing, and Assembly of BACs ... Electrophoresis was performed using a 1% agarose gel in Tris-acetate EDTA buffer for 14 h at 6 V cm−1 with an initial pulse time of 5 s and a final pulse time of 15 s. MidRange PFG Marker I (New England Biolabs) was used as a fragment size marker.

    Molecular Weight:

    Article Title: Cloning, Nucleotide Sequencing, and Functional Analysis of a Novel, Mobile Cluster of Biodegradation Genes from Pseudomonas aeruginosa Strain JB2
    Article Snippet: .. Plasmids were separated by using a CHEF-DR II (Bio-Rad Laboratories, Richmond, Calif.) run at 200 V (21 h, 14°C) with switching times ramping from 1 to 40 s. The mid-range PFG Marker II (New England Biolabs, Beverly, Mass.) was used as a molecular weight marker for linear DNA. ..

    Article Title: Evaluation of Two Approaches for Assessing the Genetic Similarity of Virioplankton Populations as Defined by Genome Size
    Article Snippet: .. Concatemers of phage lambda genomic DNA (Bio-Rad), HindIII digest of phage lambda genomic DNA (Fisher Scientific), and MidRange PFG marker (New England BioLabs) were used as molecular weight markers. .. Gels were stained in a 1:10,000 dilution of SYBR Gold (Invitrogen) for 30 min and imaged on a Typhoon 9400 variable mode imager (GE Healthcare).

    Article Title: Intercalation of small molecules into DNA in chromatin is primarily controlled by superhelical constraint
    Article Snippet: .. Gel was stained with 0.5 μg/ml ethidium bromide and imaged using FluorChem Q (Alpha Innotech, San Leandro, California, USA) gel documentation system; λ-Lambda DNA, L; Molecular Weight Marker (Midrange PFG marker New England Biolabs N0342S). ..

    Article Title: Characterization and Chromosomal Mapping of Antimicrobial Resistance Genes in Salmonella enterica Serotype Typhimurium
    Article Snippet: .. Electrophoresis was performed at 200 V using a Gene Navigator system with a hexagonal electrode array (Pharmacia LKB Biotechnology AB, Uppsala, Sweden) in the interpolation mode pulsing from 1 through 40 s. Molecular weight markers included mid-range PFG Markers (New England BioLabs, Hertfordshire, United Kingdom), and digoxigenin-labeled DNA molecular weight marker grade II (Roche Diagnostics, Lewes, East Sussex, United Kingdom). .. Gels were stained with 0.5 mg of ethidium bromide per liter in distilled water before being photographed over a UV transilluminator.

    Nucleic Acid Electrophoresis:

    Article Title: A Baculovirus alkaline nuclease knockout construct produces fragmented DNA and aberrant capsids
    Article Snippet: Paragraph title: DNA transfection, viral infection, field inversion gel electrophoresis and hybridization ... MidRange PFG marker I and Yeast chromosome PFG markers (New England Biolabs) were used as DNA size markers.

    Article Title: Characterization of a baculovirus lacking the DBP (DNA-binding protein) gene
    Article Snippet: Paragraph title: Field-inversion gel-electrophoresis ... MidRange PFG marker I (New England Biolabs) was used as DNA size markers.

    Article Title: Human Immunodeficiency Virus Replication in a Primary Effusion Lymphoma Cell Line Stimulates Lytic-Phase Replication of Kaposi's Sarcoma-Associated Herpesvirus
    Article Snippet: Paragraph title: Field inversion gel electrophoresis (FIGE) analysis of KSHV DNA. ... Two different DNA size markers, which included Lambda ladder PFG and mid-range PFG markers (New England Bio labs, Beverly, Mass.) were run on either side of the samples for precise size determination of the linear DNA.

    Fluorescence:

    Article Title: Intercalation of small molecules into DNA in chromatin is primarily controlled by superhelical constraint
    Article Snippet: Paragraph title: Supporting information Representative images and fluorescence profile of EBr stained cells/nuclei. Biphasic dependence of EBr intercalation on salt. Effect of salt treatment on nuclear size. Formaldehyde crosslinking significantly reduces EBr inte rcalation in RNA depleted, salt treated nuclei, relative to ethanol fixation. Raw image for gel blot in : Nick frequency as a function of x-ray irradiation dose. ... Gel was stained with 0.5 μg/ml ethidium bromide and imaged using FluorChem Q (Alpha Innotech, San Leandro, California, USA) gel documentation system; λ-Lambda DNA, L; Molecular Weight Marker (Midrange PFG marker New England Biolabs N0342S).

    Isolation:

    Article Title: Whole genome sequencing and comparative genomic analyses of two Vibrio cholerae O139 Bengal-specific Podoviruses to other N4-like phages reveal extensive genetic diversity
    Article Snippet: Pulsed-field gel electrophoresis was done using a CHEF-DR-III apparatus (BioRad, Hercules, CA) under the following conditions: Gels (1%) were cast using Megabase agarose (BioRad) and run at 14°C buffer temperature with 1-25 s switch time, 6 V/cm and 120° angle for 17.5 h. PFGE MidRange Marker I and II (New England Biolabs, Ipswich, MA) were used for estimating fragment sizes. .. Primers for genome-end runoff sequencing were designed based on the consensus genome sequence and Sanger sequencing reactions were carried out using either intact ϕJA1 DNA or isolated restriction fragments as templates.

    Labeling:

    Article Title: A Baculovirus alkaline nuclease knockout construct produces fragmented DNA and aberrant capsids
    Article Snippet: MidRange PFG marker I and Yeast chromosome PFG markers (New England Biolabs) were used as DNA size markers. .. The DNA was then transferred to a nylon membrane and hybridized with viral genomic DNA digested with Eco RI and Hind III that was labeled using the Alk-Phos Direct Labeling and Detection system (Amersham) as described previously ( ).

    Article Title: Characterization of a baculovirus lacking the DBP (DNA-binding protein) gene
    Article Snippet: MidRange PFG marker I (New England Biolabs) was used as DNA size markers. .. The DNA was transferred to a nylon membrane and hybridized with viral genomic DNA using the alkaline phosphate direct labeling system (Amersham) as described previously ( ) or by 32 P-labeled bacmid DNA.

    Purification:

    Article Title: Isolation and Characterization of Bacteriophages Infecting the Fish Pathogen Flavobacterium psychrophilum
    Article Snippet: The genome sizes of bacteriophages were determined by pulsed-field gel electrophoresis (PFGE) of purified bacteriophage DNA ( ). .. The PFGE size standards used were an 8- to 48-kb standard (Bio-Rad) and MidRange PFGE Marker I (New England Biolabs).

    Polymerase Chain Reaction:

    Article Title: The temperate Burkholderia phage AP3 of the Peduovirinae shows efficient antimicrobial activity against B. cenocepacia of the IIIA lineage
    Article Snippet: Type IIIA and IIIB lineages among B. cenocepacia strains were identified by PCR (Mahenthiralingam et al. ). .. A linear ramp of 5 to 65 s was used, and gels were run for 20 h at 200 V. MidRange PFGE Marker (New England Biolabs) was used as a mass marker.

    Article Title: Dynamic Rearrangements Determine Genome Organization and Useful Traits in Soybean 1Dynamic Rearrangements Determine Genome Organization and Useful Traits in Soybean 1 [C]Dynamic Rearrangements Determine Genome Organization and Useful Traits in Soybean 1 [C] [W]
    Article Snippet: PCR-based screening was conducted with multidimensional pools of the Williams 82 soybean BAC library ( ) using the same conditions as described above. .. Electrophoresis was performed using a 1% agarose gel in Tris-acetate EDTA buffer for 14 h at 6 V cm−1 with an initial pulse time of 5 s and a final pulse time of 15 s. MidRange PFG Marker I (New England Biolabs) was used as a fragment size marker.

    Lysis:

    Article Title: The temperate Burkholderia phage AP3 of the Peduovirinae shows efficient antimicrobial activity against B. cenocepacia of the IIIA lineage
    Article Snippet: PFGE was carried out according to Chua et al. ( ) using a one-step lysis procedure in which bacterial cells, immobilized in an agarose gel, were lysed in lysis buffer containing 50 mM Tris, 50 mM EDTA, 1 % SDS, and 100 μg/ml proteinase K (pH 8.0) at 56 °C for 2 h. Next, agarose plugs were thoroughly rinsed with TE buffer (10 mM Tris, 1 mM EDTA, pH 8.0) and digested with Xba I (Thermo Fisher Scientific, Fermentas Life Science, Waltham, MA, USA). .. A linear ramp of 5 to 65 s was used, and gels were run for 20 h at 200 V. MidRange PFGE Marker (New England Biolabs) was used as a mass marker.

    Article Title: Evaluation of Two Approaches for Assessing the Genetic Similarity of Virioplankton Populations as Defined by Genome Size
    Article Snippet: The DNA was liberated within the plugs via overnight lysis (30°C in the dark with a lysis buffer [250 mM EDTA, pH 8], 1% SDS, and 1 mg/ml proteinase K solution) ( ). .. Concatemers of phage lambda genomic DNA (Bio-Rad), HindIII digest of phage lambda genomic DNA (Fisher Scientific), and MidRange PFG marker (New England BioLabs) were used as molecular weight markers.

    Article Title: Characterization and Chromosomal Mapping of Antimicrobial Resistance Genes in Salmonella enterica Serotype Typhimurium
    Article Snippet: Cell lysis was achieved using a mixture containing Lysozyme (Sigma, Poole, United Kingdom) at a final concentration of 0.1 mg/ml and RNase (Sigma) at a final concentration of 20 μg/ml in lysis buffer (6 mM Tris [pH 7.6], 1 M NaCl, 100 mM EDTA, 0.5% Brij-58 [polyoxyethylene-20-cetyl-ether], 0.2% sodium deoxycholate, 0.5% sodium lauroyl sarcosine). .. Electrophoresis was performed at 200 V using a Gene Navigator system with a hexagonal electrode array (Pharmacia LKB Biotechnology AB, Uppsala, Sweden) in the interpolation mode pulsing from 1 through 40 s. Molecular weight markers included mid-range PFG Markers (New England BioLabs, Hertfordshire, United Kingdom), and digoxigenin-labeled DNA molecular weight marker grade II (Roche Diagnostics, Lewes, East Sussex, United Kingdom).

    Plasmid Preparation:

    Article Title: Cloning, Nucleotide Sequencing, and Functional Analysis of a Novel, Mobile Cluster of Biodegradation Genes from Pseudomonas aeruginosa Strain JB2
    Article Snippet: Plasmid profiles were analyzed by pulsed-field gel electrophoresis (PFGE) by the contour-clamped homogenous electric field (CHEF) technique. .. Plasmids were separated by using a CHEF-DR II (Bio-Rad Laboratories, Richmond, Calif.) run at 200 V (21 h, 14°C) with switching times ramping from 1 to 40 s. The mid-range PFG Marker II (New England Biolabs, Beverly, Mass.) was used as a molecular weight marker for linear DNA.

    Irradiation:

    Article Title: Intercalation of small molecules into DNA in chromatin is primarily controlled by superhelical constraint
    Article Snippet: Paragraph title: In-gel cell irradiation and determination of nick incidence ... Fragment size was identified using a pulse marker (Midrange PFG marker New England Biolabs N0342S) that was run alongside the samples.

    Agarose Gel Electrophoresis:

    Article Title: Intercalation of small molecules into DNA in chromatin is primarily controlled by superhelical constraint
    Article Snippet: Double-stranded DNA fragments (3–300 kb) were separated by CHEF (Contour-clamped Homogenous Electric Field electrophoresis) in a 1% agarose gel in 0.5x TBE buffer (89 mM boric acid, 89 mM Tris base and 2mM EDTA) using a CHEF-mapper (Bio RAD). .. Fragment size was identified using a pulse marker (Midrange PFG marker New England Biolabs N0342S) that was run alongside the samples.

    Article Title: A Baculovirus alkaline nuclease knockout construct produces fragmented DNA and aberrant capsids
    Article Snippet: Agarose plugs were placed into the wells of 1% Pulsed Field Certified Agarose gel (Bio-Rad) in 0.5 × TBE buffer (45mM Tris-borate, pH 8.0, 1 mM EDTA) and separated by field inversion gel electrophoresis (FIGE) using a MJ Research PPI-200 programmable pulse inverter. .. MidRange PFG marker I and Yeast chromosome PFG markers (New England Biolabs) were used as DNA size markers.

    Article Title: The temperate Burkholderia phage AP3 of the Peduovirinae shows efficient antimicrobial activity against B. cenocepacia of the IIIA lineage
    Article Snippet: DNA fragments were separated by PFGE on a CHEF DRII apparatus (Bio-Rad Laboratories, Hemel Hempstead, UK) in a 1 % agarose gel in 0.5 × TBE buffer at 12 °C. .. A linear ramp of 5 to 65 s was used, and gels were run for 20 h at 200 V. MidRange PFGE Marker (New England Biolabs) was used as a mass marker.

    Article Title: Identification of Bacteriophages for Biocontrol of the Kiwifruit Canker Phytopathogen Pseudomonas syringae pv. actinidiae
    Article Snippet: To estimate genome size, phage DNA was loaded into a 1% pulsed-field gel electrophoresis (PFGE)-certified agarose gel, and the wells were sealed with agarose. .. Conditions for electrophoresis were as follows: included angle of 120°, initial switch time of 5 s, and final switch time of 15 s. Midrange PFG Marker I (NEB) was used as the ladder.

    Article Title: Whole genome sequencing and comparative genomic analyses of two Vibrio cholerae O139 Bengal-specific Podoviruses to other N4-like phages reveal extensive genetic diversity
    Article Snippet: The presence of putative cohesive genomic ends was evaluated by digesting genomic DNA with Cla I, Van 91I and Oli I, heating half of the digest to 75°C for 10 min and comparing it to the non-heated restriction digests after electrophoresis in an agarose gel. .. Pulsed-field gel electrophoresis was done using a CHEF-DR-III apparatus (BioRad, Hercules, CA) under the following conditions: Gels (1%) were cast using Megabase agarose (BioRad) and run at 14°C buffer temperature with 1-25 s switch time, 6 V/cm and 120° angle for 17.5 h. PFGE MidRange Marker I and II (New England Biolabs, Ipswich, MA) were used for estimating fragment sizes.

    Article Title: virF-Positive Yersinia pseudotuberculosis and Yersinia enterocolitica Found in Migratory Birds in Sweden †
    Article Snippet: The samples were electrophoresed at 12°C through a 1% (wt/vol) agarose gel (SeaKem Gold; FMC Bioproducts) in a 0.5× Tris-borate-EDTA buffer (Amresco, Solon, Ohio) at 200 V by using a Gene Navigator system (Pharmacia, Uppsala, Sweden) with a hexagonal electrode. .. A midrange PFGE marker (New England Biolabs) was used for fragment size determination.

    Article Title: Characterization and Chromosomal Mapping of Antimicrobial Resistance Genes in Salmonella enterica Serotype Typhimurium
    Article Snippet: Samples were electrophoresed in a 1% (wt/vol) agarose gel (SeaKem Gold; FMC Bioproducts) in 0.5× TBE (45 mM Tris, 45 mM borate [pH 8.3], 1 mM EDTA) for 20 h at 10°C. .. Electrophoresis was performed at 200 V using a Gene Navigator system with a hexagonal electrode array (Pharmacia LKB Biotechnology AB, Uppsala, Sweden) in the interpolation mode pulsing from 1 through 40 s. Molecular weight markers included mid-range PFG Markers (New England BioLabs, Hertfordshire, United Kingdom), and digoxigenin-labeled DNA molecular weight marker grade II (Roche Diagnostics, Lewes, East Sussex, United Kingdom).

    Article Title: Dynamic Rearrangements Determine Genome Organization and Useful Traits in Soybean 1Dynamic Rearrangements Determine Genome Organization and Useful Traits in Soybean 1 [C]Dynamic Rearrangements Determine Genome Organization and Useful Traits in Soybean 1 [C] [W]
    Article Snippet: .. Electrophoresis was performed using a 1% agarose gel in Tris-acetate EDTA buffer for 14 h at 6 V cm−1 with an initial pulse time of 5 s and a final pulse time of 15 s. MidRange PFG Marker I (New England Biolabs) was used as a fragment size marker. ..

    Electrophoresis:

    Article Title: Isolation and Characterization of Bacteriophages Infecting the Fish Pathogen Flavobacterium psychrophilum
    Article Snippet: After electrophoresis, the DNA was visualized by staining with Sybr green I in 0.5× Tris-borate-EDTA. .. The PFGE size standards used were an 8- to 48-kb standard (Bio-Rad) and MidRange PFGE Marker I (New England Biolabs).

    Article Title: Intercalation of small molecules into DNA in chromatin is primarily controlled by superhelical constraint
    Article Snippet: Double-stranded DNA fragments (3–300 kb) were separated by CHEF (Contour-clamped Homogenous Electric Field electrophoresis) in a 1% agarose gel in 0.5x TBE buffer (89 mM boric acid, 89 mM Tris base and 2mM EDTA) using a CHEF-mapper (Bio RAD). .. Fragment size was identified using a pulse marker (Midrange PFG marker New England Biolabs N0342S) that was run alongside the samples.

    Article Title: Identification of Bacteriophages for Biocontrol of the Kiwifruit Canker Phytopathogen Pseudomonas syringae pv. actinidiae
    Article Snippet: .. Conditions for electrophoresis were as follows: included angle of 120°, initial switch time of 5 s, and final switch time of 15 s. Midrange PFG Marker I (NEB) was used as the ladder. ..

    Article Title: Evaluation of Two Approaches for Assessing the Genetic Similarity of Virioplankton Populations as Defined by Genome Size
    Article Snippet: The size of viral genomic DNA bands was determined on the basis of three different electrophoresis runs for each viral concentrate. .. Concatemers of phage lambda genomic DNA (Bio-Rad), HindIII digest of phage lambda genomic DNA (Fisher Scientific), and MidRange PFG marker (New England BioLabs) were used as molecular weight markers.

    Article Title: Intercalation of small molecules into DNA in chromatin is primarily controlled by superhelical constraint
    Article Snippet: The DNA samples were analysed on agarose gels by CHEF electrophoresis. .. Gel was stained with 0.5 μg/ml ethidium bromide and imaged using FluorChem Q (Alpha Innotech, San Leandro, California, USA) gel documentation system; λ-Lambda DNA, L; Molecular Weight Marker (Midrange PFG marker New England Biolabs N0342S).

    Article Title: Whole genome sequencing and comparative genomic analyses of two Vibrio cholerae O139 Bengal-specific Podoviruses to other N4-like phages reveal extensive genetic diversity
    Article Snippet: The presence of putative cohesive genomic ends was evaluated by digesting genomic DNA with Cla I, Van 91I and Oli I, heating half of the digest to 75°C for 10 min and comparing it to the non-heated restriction digests after electrophoresis in an agarose gel. .. Pulsed-field gel electrophoresis was done using a CHEF-DR-III apparatus (BioRad, Hercules, CA) under the following conditions: Gels (1%) were cast using Megabase agarose (BioRad) and run at 14°C buffer temperature with 1-25 s switch time, 6 V/cm and 120° angle for 17.5 h. PFGE MidRange Marker I and II (New England Biolabs, Ipswich, MA) were used for estimating fragment sizes.

    Article Title: Characterization of a baculovirus lacking the DBP (DNA-binding protein) gene
    Article Snippet: To prepare DNA for electrophoresis, agarose pieces were digested with 30U of Eco 81I and/or 10U of Dpn I in 100 μl of reaction volume overnight. .. MidRange PFG marker I (New England Biolabs) was used as DNA size markers.

    Article Title: Characterization and Chromosomal Mapping of Antimicrobial Resistance Genes in Salmonella enterica Serotype Typhimurium
    Article Snippet: .. Electrophoresis was performed at 200 V using a Gene Navigator system with a hexagonal electrode array (Pharmacia LKB Biotechnology AB, Uppsala, Sweden) in the interpolation mode pulsing from 1 through 40 s. Molecular weight markers included mid-range PFG Markers (New England BioLabs, Hertfordshire, United Kingdom), and digoxigenin-labeled DNA molecular weight marker grade II (Roche Diagnostics, Lewes, East Sussex, United Kingdom). .. Gels were stained with 0.5 mg of ethidium bromide per liter in distilled water before being photographed over a UV transilluminator.

    Article Title: Dynamic Rearrangements Determine Genome Organization and Useful Traits in Soybean 1Dynamic Rearrangements Determine Genome Organization and Useful Traits in Soybean 1 [C]Dynamic Rearrangements Determine Genome Organization and Useful Traits in Soybean 1 [C] [W]
    Article Snippet: .. Electrophoresis was performed using a 1% agarose gel in Tris-acetate EDTA buffer for 14 h at 6 V cm−1 with an initial pulse time of 5 s and a final pulse time of 15 s. MidRange PFG Marker I (New England Biolabs) was used as a fragment size marker. ..

    Article Title: Human Immunodeficiency Virus Replication in a Primary Effusion Lymphoma Cell Line Stimulates Lytic-Phase Replication of Kaposi's Sarcoma-Associated Herpesvirus
    Article Snippet: Samples were run on 1% pulsed-field gel electrophoresis-certified agarose (Bio-Rad) gels in 0.5× Tris-borate-EDTA at 150 V (forward) or 50 V (reverse), ramped from 3 to 30 s for 20 h at 15°C with a peristaltic pump that circulated buffer constantly through the FIGE Mapper Cell (Bio-Rad). .. Two different DNA size markers, which included Lambda ladder PFG and mid-range PFG markers (New England Bio labs, Beverly, Mass.) were run on either side of the samples for precise size determination of the linear DNA.

    Concentration Assay:

    Article Title: Characterization and Chromosomal Mapping of Antimicrobial Resistance Genes in Salmonella enterica Serotype Typhimurium
    Article Snippet: Restriction endonuclease digestion was carried out on 1- to 2-mm slices of each prechilled gel mold by adding 5 U of Xba I in multicore buffer (final concentration, 1×), bovine serum albumin (final concentration, 0.1 mg/ml; Promega) to a final volume of 100 μl with sterile distilled water. .. Electrophoresis was performed at 200 V using a Gene Navigator system with a hexagonal electrode array (Pharmacia LKB Biotechnology AB, Uppsala, Sweden) in the interpolation mode pulsing from 1 through 40 s. Molecular weight markers included mid-range PFG Markers (New England BioLabs, Hertfordshire, United Kingdom), and digoxigenin-labeled DNA molecular weight marker grade II (Roche Diagnostics, Lewes, East Sussex, United Kingdom).

    Pulsed-Field Gel:

    Article Title: Isolation and Characterization of Bacteriophages Infecting the Fish Pathogen Flavobacterium psychrophilum
    Article Snippet: The genome sizes of bacteriophages were determined by pulsed-field gel electrophoresis (PFGE) of purified bacteriophage DNA ( ). .. The PFGE size standards used were an 8- to 48-kb standard (Bio-Rad) and MidRange PFGE Marker I (New England Biolabs).

    Article Title: The temperate Burkholderia phage AP3 of the Peduovirinae shows efficient antimicrobial activity against B. cenocepacia of the IIIA lineage
    Article Snippet: Pulsed-field gel electrophoresis (PFGE) was employed to discriminate the clonal origin of B. cenocepacia IIIA isolates sensitive to AP3 infection. .. A linear ramp of 5 to 65 s was used, and gels were run for 20 h at 200 V. MidRange PFGE Marker (New England Biolabs) was used as a mass marker.

    Article Title: Identification of Bacteriophages for Biocontrol of the Kiwifruit Canker Phytopathogen Pseudomonas syringae pv. actinidiae
    Article Snippet: To estimate genome size, phage DNA was loaded into a 1% pulsed-field gel electrophoresis (PFGE)-certified agarose gel, and the wells were sealed with agarose. .. Conditions for electrophoresis were as follows: included angle of 120°, initial switch time of 5 s, and final switch time of 15 s. Midrange PFG Marker I (NEB) was used as the ladder.

    Article Title: Cloning, Nucleotide Sequencing, and Functional Analysis of a Novel, Mobile Cluster of Biodegradation Genes from Pseudomonas aeruginosa Strain JB2
    Article Snippet: Plasmid profiles were analyzed by pulsed-field gel electrophoresis (PFGE) by the contour-clamped homogenous electric field (CHEF) technique. .. Plasmids were separated by using a CHEF-DR II (Bio-Rad Laboratories, Richmond, Calif.) run at 200 V (21 h, 14°C) with switching times ramping from 1 to 40 s. The mid-range PFG Marker II (New England Biolabs, Beverly, Mass.) was used as a molecular weight marker for linear DNA.

    Article Title: Whole genome sequencing and comparative genomic analyses of two Vibrio cholerae O139 Bengal-specific Podoviruses to other N4-like phages reveal extensive genetic diversity
    Article Snippet: .. Pulsed-field gel electrophoresis was done using a CHEF-DR-III apparatus (BioRad, Hercules, CA) under the following conditions: Gels (1%) were cast using Megabase agarose (BioRad) and run at 14°C buffer temperature with 1-25 s switch time, 6 V/cm and 120° angle for 17.5 h. PFGE MidRange Marker I and II (New England Biolabs, Ipswich, MA) were used for estimating fragment sizes. .. Primers for genome-end runoff sequencing were designed based on the consensus genome sequence and Sanger sequencing reactions were carried out using either intact ϕJA1 DNA or isolated restriction fragments as templates.

    Article Title: Human Immunodeficiency Virus Replication in a Primary Effusion Lymphoma Cell Line Stimulates Lytic-Phase Replication of Kaposi's Sarcoma-Associated Herpesvirus
    Article Snippet: Samples were run on 1% pulsed-field gel electrophoresis-certified agarose (Bio-Rad) gels in 0.5× Tris-borate-EDTA at 150 V (forward) or 50 V (reverse), ramped from 3 to 30 s for 20 h at 15°C with a peristaltic pump that circulated buffer constantly through the FIGE Mapper Cell (Bio-Rad). .. Two different DNA size markers, which included Lambda ladder PFG and mid-range PFG markers (New England Bio labs, Beverly, Mass.) were run on either side of the samples for precise size determination of the linear DNA.

    BAC Assay:

    Article Title: Dynamic Rearrangements Determine Genome Organization and Useful Traits in Soybean 1Dynamic Rearrangements Determine Genome Organization and Useful Traits in Soybean 1 [C]Dynamic Rearrangements Determine Genome Organization and Useful Traits in Soybean 1 [C] [W]
    Article Snippet: The insert size of the BAC clone was estimated by contour-clamped homogeneous electric field electrophoresis after Not I digestion. .. Electrophoresis was performed using a 1% agarose gel in Tris-acetate EDTA buffer for 14 h at 6 V cm−1 with an initial pulse time of 5 s and a final pulse time of 15 s. MidRange PFG Marker I (New England Biolabs) was used as a fragment size marker.

    Marker:

    Article Title: Isolation and Characterization of Bacteriophages Infecting the Fish Pathogen Flavobacterium psychrophilum
    Article Snippet: .. The PFGE size standards used were an 8- to 48-kb standard (Bio-Rad) and MidRange PFGE Marker I (New England Biolabs). .. The host ranges of the isolated bacteriophages were determined by spotting 2 μl of bacteriophage concentrate on top of a TYES-A plate freshly prepared with 4 ml of top agar inoculated with 0.3 ml of the strain to be tested.

    Article Title: Intercalation of small molecules into DNA in chromatin is primarily controlled by superhelical constraint
    Article Snippet: .. Fragment size was identified using a pulse marker (Midrange PFG marker New England Biolabs N0342S) that was run alongside the samples. ..

    Article Title: A Baculovirus alkaline nuclease knockout construct produces fragmented DNA and aberrant capsids
    Article Snippet: .. MidRange PFG marker I and Yeast chromosome PFG markers (New England Biolabs) were used as DNA size markers. .. For Southern blot analysis, the agarose gel was soaked in 0.25M HCl for 15 min followed by 0.4M NaOH for 15 min.

    Article Title: The temperate Burkholderia phage AP3 of the Peduovirinae shows efficient antimicrobial activity against B. cenocepacia of the IIIA lineage
    Article Snippet: .. A linear ramp of 5 to 65 s was used, and gels were run for 20 h at 200 V. MidRange PFGE Marker (New England Biolabs) was used as a mass marker. .. DNA was stained with ethidium bromide.

    Article Title: Identification of Bacteriophages for Biocontrol of the Kiwifruit Canker Phytopathogen Pseudomonas syringae pv. actinidiae
    Article Snippet: .. Conditions for electrophoresis were as follows: included angle of 120°, initial switch time of 5 s, and final switch time of 15 s. Midrange PFG Marker I (NEB) was used as the ladder. ..

    Article Title: Cloning, Nucleotide Sequencing, and Functional Analysis of a Novel, Mobile Cluster of Biodegradation Genes from Pseudomonas aeruginosa Strain JB2
    Article Snippet: .. Plasmids were separated by using a CHEF-DR II (Bio-Rad Laboratories, Richmond, Calif.) run at 200 V (21 h, 14°C) with switching times ramping from 1 to 40 s. The mid-range PFG Marker II (New England Biolabs, Beverly, Mass.) was used as a molecular weight marker for linear DNA. ..

    Article Title: Evaluation of Two Approaches for Assessing the Genetic Similarity of Virioplankton Populations as Defined by Genome Size
    Article Snippet: .. Concatemers of phage lambda genomic DNA (Bio-Rad), HindIII digest of phage lambda genomic DNA (Fisher Scientific), and MidRange PFG marker (New England BioLabs) were used as molecular weight markers. .. Gels were stained in a 1:10,000 dilution of SYBR Gold (Invitrogen) for 30 min and imaged on a Typhoon 9400 variable mode imager (GE Healthcare).

    Article Title: Intercalation of small molecules into DNA in chromatin is primarily controlled by superhelical constraint
    Article Snippet: .. Gel was stained with 0.5 μg/ml ethidium bromide and imaged using FluorChem Q (Alpha Innotech, San Leandro, California, USA) gel documentation system; λ-Lambda DNA, L; Molecular Weight Marker (Midrange PFG marker New England Biolabs N0342S). ..

    Article Title: Whole genome sequencing and comparative genomic analyses of two Vibrio cholerae O139 Bengal-specific Podoviruses to other N4-like phages reveal extensive genetic diversity
    Article Snippet: .. Pulsed-field gel electrophoresis was done using a CHEF-DR-III apparatus (BioRad, Hercules, CA) under the following conditions: Gels (1%) were cast using Megabase agarose (BioRad) and run at 14°C buffer temperature with 1-25 s switch time, 6 V/cm and 120° angle for 17.5 h. PFGE MidRange Marker I and II (New England Biolabs, Ipswich, MA) were used for estimating fragment sizes. .. Primers for genome-end runoff sequencing were designed based on the consensus genome sequence and Sanger sequencing reactions were carried out using either intact ϕJA1 DNA or isolated restriction fragments as templates.

    Article Title: Characterization of a baculovirus lacking the DBP (DNA-binding protein) gene
    Article Snippet: .. MidRange PFG marker I (New England Biolabs) was used as DNA size markers. .. The DNA was transferred to a nylon membrane and hybridized with viral genomic DNA using the alkaline phosphate direct labeling system (Amersham) as described previously ( ) or by 32 P-labeled bacmid DNA.

    Article Title: Purification and partial genome characterization of the bacterial endosymbiont Blattabacterium cuenoti from the fat bodies of cockroaches
    Article Snippet: .. M2: MidRange PFG Marker I (New England Biolabs). ..

    Article Title: virF-Positive Yersinia pseudotuberculosis and Yersinia enterocolitica Found in Migratory Birds in Sweden †
    Article Snippet: .. A midrange PFGE marker (New England Biolabs) was used for fragment size determination. .. The gels were stained for 30 min in 1 liter of running buffer containing 50 μl of ethidium bromide (10 mg/ml) and photographed under UV light with an Alpha Imager 2000 documentation system (Alpha Innotech, San Leandro, Calif.) by following standard procedures.

    Article Title: Characterization and Chromosomal Mapping of Antimicrobial Resistance Genes in Salmonella enterica Serotype Typhimurium
    Article Snippet: .. Electrophoresis was performed at 200 V using a Gene Navigator system with a hexagonal electrode array (Pharmacia LKB Biotechnology AB, Uppsala, Sweden) in the interpolation mode pulsing from 1 through 40 s. Molecular weight markers included mid-range PFG Markers (New England BioLabs, Hertfordshire, United Kingdom), and digoxigenin-labeled DNA molecular weight marker grade II (Roche Diagnostics, Lewes, East Sussex, United Kingdom). .. Gels were stained with 0.5 mg of ethidium bromide per liter in distilled water before being photographed over a UV transilluminator.

    Article Title: Dynamic Rearrangements Determine Genome Organization and Useful Traits in Soybean 1Dynamic Rearrangements Determine Genome Organization and Useful Traits in Soybean 1 [C]Dynamic Rearrangements Determine Genome Organization and Useful Traits in Soybean 1 [C] [W]
    Article Snippet: .. Electrophoresis was performed using a 1% agarose gel in Tris-acetate EDTA buffer for 14 h at 6 V cm−1 with an initial pulse time of 5 s and a final pulse time of 15 s. MidRange PFG Marker I (New England Biolabs) was used as a fragment size marker. ..

    Staining:

    Article Title: Isolation and Characterization of Bacteriophages Infecting the Fish Pathogen Flavobacterium psychrophilum
    Article Snippet: After electrophoresis, the DNA was visualized by staining with Sybr green I in 0.5× Tris-borate-EDTA. .. The PFGE size standards used were an 8- to 48-kb standard (Bio-Rad) and MidRange PFGE Marker I (New England Biolabs).

    Article Title: Intercalation of small molecules into DNA in chromatin is primarily controlled by superhelical constraint
    Article Snippet: The gel was stained with 0.5 μg/ml EBr and imaged using FluorChem Q (Alpha Innotech, San Leandro, California, USA) gel documentation system. .. Fragment size was identified using a pulse marker (Midrange PFG marker New England Biolabs N0342S) that was run alongside the samples.

    Article Title: The temperate Burkholderia phage AP3 of the Peduovirinae shows efficient antimicrobial activity against B. cenocepacia of the IIIA lineage
    Article Snippet: A linear ramp of 5 to 65 s was used, and gels were run for 20 h at 200 V. MidRange PFGE Marker (New England Biolabs) was used as a mass marker. .. DNA was stained with ethidium bromide.

    Article Title: Evaluation of Two Approaches for Assessing the Genetic Similarity of Virioplankton Populations as Defined by Genome Size
    Article Snippet: Concatemers of phage lambda genomic DNA (Bio-Rad), HindIII digest of phage lambda genomic DNA (Fisher Scientific), and MidRange PFG marker (New England BioLabs) were used as molecular weight markers. .. Gels were stained in a 1:10,000 dilution of SYBR Gold (Invitrogen) for 30 min and imaged on a Typhoon 9400 variable mode imager (GE Healthcare).

    Article Title: Intercalation of small molecules into DNA in chromatin is primarily controlled by superhelical constraint
    Article Snippet: .. Gel was stained with 0.5 μg/ml ethidium bromide and imaged using FluorChem Q (Alpha Innotech, San Leandro, California, USA) gel documentation system; λ-Lambda DNA, L; Molecular Weight Marker (Midrange PFG marker New England Biolabs N0342S). ..

    Article Title: virF-Positive Yersinia pseudotuberculosis and Yersinia enterocolitica Found in Migratory Birds in Sweden †
    Article Snippet: A midrange PFGE marker (New England Biolabs) was used for fragment size determination. .. The gels were stained for 30 min in 1 liter of running buffer containing 50 μl of ethidium bromide (10 mg/ml) and photographed under UV light with an Alpha Imager 2000 documentation system (Alpha Innotech, San Leandro, Calif.) by following standard procedures.

    Article Title: Characterization and Chromosomal Mapping of Antimicrobial Resistance Genes in Salmonella enterica Serotype Typhimurium
    Article Snippet: Electrophoresis was performed at 200 V using a Gene Navigator system with a hexagonal electrode array (Pharmacia LKB Biotechnology AB, Uppsala, Sweden) in the interpolation mode pulsing from 1 through 40 s. Molecular weight markers included mid-range PFG Markers (New England BioLabs, Hertfordshire, United Kingdom), and digoxigenin-labeled DNA molecular weight marker grade II (Roche Diagnostics, Lewes, East Sussex, United Kingdom). .. Gels were stained with 0.5 mg of ethidium bromide per liter in distilled water before being photographed over a UV transilluminator.

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    New England Biolabs midrange pfg marker
    Pulsed-field gels of virioplankton concentrates. Gels are labeled according to the month and year of sample collection. Lanes are labeled by station designation in the Chesapeake Bay (CB) and Delaware Bay (DB). Samples from the June 2006 and July 2007 cruises were loaded at two different concentrations of virus particles: 10 9 (A) and ∼10 10 (B). <t>DNA</t> was purified from bands marked with numbers. Marker lanes (in kilobases) are as follows: M1, concatemers of phage λ genome mixed with HindIII digest of λ genomic DNA; M2, concatemers of phage λ genome; M3, λ DNA digested with HindIII; M4, midrange <t>PFG</t> marker.
    Midrange Pfg Marker, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/midrange pfg marker/product/New England Biolabs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    midrange pfg marker - by Bioz Stars, 2020-01
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    Pulsed-field gels of virioplankton concentrates. Gels are labeled according to the month and year of sample collection. Lanes are labeled by station designation in the Chesapeake Bay (CB) and Delaware Bay (DB). Samples from the June 2006 and July 2007 cruises were loaded at two different concentrations of virus particles: 10 9 (A) and ∼10 10 (B). DNA was purified from bands marked with numbers. Marker lanes (in kilobases) are as follows: M1, concatemers of phage λ genome mixed with HindIII digest of λ genomic DNA; M2, concatemers of phage λ genome; M3, λ DNA digested with HindIII; M4, midrange PFG marker.

    Journal: Applied and Environmental Microbiology

    Article Title: Evaluation of Two Approaches for Assessing the Genetic Similarity of Virioplankton Populations as Defined by Genome Size

    doi: 10.1128/AEM.02432-12

    Figure Lengend Snippet: Pulsed-field gels of virioplankton concentrates. Gels are labeled according to the month and year of sample collection. Lanes are labeled by station designation in the Chesapeake Bay (CB) and Delaware Bay (DB). Samples from the June 2006 and July 2007 cruises were loaded at two different concentrations of virus particles: 10 9 (A) and ∼10 10 (B). DNA was purified from bands marked with numbers. Marker lanes (in kilobases) are as follows: M1, concatemers of phage λ genome mixed with HindIII digest of λ genomic DNA; M2, concatemers of phage λ genome; M3, λ DNA digested with HindIII; M4, midrange PFG marker.

    Article Snippet: Concatemers of phage lambda genomic DNA (Bio-Rad), HindIII digest of phage lambda genomic DNA (Fisher Scientific), and MidRange PFG marker (New England BioLabs) were used as molecular weight markers.

    Techniques: Labeling, Purification, Marker