lambda ladder pfge  (New England Biolabs)


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    Name:
    Lambda PFG Ladder
    Description:
    Lambda PFG Ladder 50 gel lanes
    Catalog Number:
    n0341s
    Price:
    156
    Size:
    50 gel lanes
    Category:
    DNA Ladders
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    Structured Review

    New England Biolabs lambda ladder pfge
    Lambda PFG Ladder
    Lambda PFG Ladder 50 gel lanes
    https://www.bioz.com/result/lambda ladder pfge/product/New England Biolabs
    Average 95 stars, based on 42 article reviews
    Price from $9.99 to $1999.99
    lambda ladder pfge - by Bioz Stars, 2020-02
    95/100 stars

    Images

    1) Product Images from "Biofilm-Forming Clinical Staphylococcus Isolates Harbor Horizontal Transfer and Antibiotic Resistance Genes"

    Article Title: Biofilm-Forming Clinical Staphylococcus Isolates Harbor Horizontal Transfer and Antibiotic Resistance Genes

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2017.02018

    Detection of plasmids in 25 staphylococcal clinical isolates by PFGE. Large plasmids ( > 30 kb) were analyzed by PFGE after digestion with nuclease S1 at 37°C for 45 min. Lanes 1–25: clinical isolates; lane number corresponds to the number of the isolate. Lane C: positive control, plasmid pSK41 (46.4 kb) extracted from SK5428 strain. Lane M: Lambda Ladder PFGE molecular size marker. Arrows point to detected plasmids.
    Figure Legend Snippet: Detection of plasmids in 25 staphylococcal clinical isolates by PFGE. Large plasmids ( > 30 kb) were analyzed by PFGE after digestion with nuclease S1 at 37°C for 45 min. Lanes 1–25: clinical isolates; lane number corresponds to the number of the isolate. Lane C: positive control, plasmid pSK41 (46.4 kb) extracted from SK5428 strain. Lane M: Lambda Ladder PFGE molecular size marker. Arrows point to detected plasmids.

    Techniques Used: Positive Control, Plasmid Preparation, Marker

    2) Product Images from "Discovery of Alternative Producers of the Enediyne Antitumor Antibiotic C-1027 with High Titers"

    Article Title: Discovery of Alternative Producers of the Enediyne Antitumor Antibiotic C-1027 with High Titers

    Journal: Journal of natural products

    doi: 10.1021/acs.jnatprod.7b01013

    C-1027 BGCs residing on giant plasmids of varying size in the five C-1027 producers. (A) PFGE analysis of the five C-1027 producers showing the existence of giant plasmids of varying sizes. (B) Southern analysis revealing that the C-1027 BGCs all reside on giant plasmids in the five producers. M, Lambda PFG ladder (NEB); lane 1, S. globisporus wild-type; lane 2, S. globisporus AF40; lane 3, CB00657; lane 4, CB02329; lane 5, CB02366; and lane 6, CB03608.
    Figure Legend Snippet: C-1027 BGCs residing on giant plasmids of varying size in the five C-1027 producers. (A) PFGE analysis of the five C-1027 producers showing the existence of giant plasmids of varying sizes. (B) Southern analysis revealing that the C-1027 BGCs all reside on giant plasmids in the five producers. M, Lambda PFG ladder (NEB); lane 1, S. globisporus wild-type; lane 2, S. globisporus AF40; lane 3, CB00657; lane 4, CB02329; lane 5, CB02366; and lane 6, CB03608.

    Techniques Used:

    3) Product Images from "Characteristics of Vibrio parahaemolyticus O3:K6 from Asia"

    Article Title: Characteristics of Vibrio parahaemolyticus O3:K6 from Asia

    Journal: Applied and Environmental Microbiology

    doi:

    PFGE patterns of recently isolated O3:K6 strains of V. parahaemolyticus . Conditions for PFGE were 1% agarose gel, 0.5× Tris-borate-EDTA buffer, 190 V, pulse time 3 to 80 s, for 22.4 h. Lane 1, isolate 1020 (from Philippines; pattern I5); lane 2, isolate 1021 (from Singapore; pattern I6); lane 3, isolate 1084 (from Taiwan; pattern I7); lane 4, isolate 1104 (from Taiwan; pattern I8); lane 5, isolate 1123 (from Taiwan; pattern I1); lane 6, isolate 1125 (from Taiwan; pattern I1); lane 7, isolate 1139 (from Taiwan; pattern I4); lane 8, isolate 1154 (from Taiwan; pattern I2); lane 9, isolate 97-804 (from Korea; pattern I3); lane M, lambda ladder PFGE marker.
    Figure Legend Snippet: PFGE patterns of recently isolated O3:K6 strains of V. parahaemolyticus . Conditions for PFGE were 1% agarose gel, 0.5× Tris-borate-EDTA buffer, 190 V, pulse time 3 to 80 s, for 22.4 h. Lane 1, isolate 1020 (from Philippines; pattern I5); lane 2, isolate 1021 (from Singapore; pattern I6); lane 3, isolate 1084 (from Taiwan; pattern I7); lane 4, isolate 1104 (from Taiwan; pattern I8); lane 5, isolate 1123 (from Taiwan; pattern I1); lane 6, isolate 1125 (from Taiwan; pattern I1); lane 7, isolate 1139 (from Taiwan; pattern I4); lane 8, isolate 1154 (from Taiwan; pattern I2); lane 9, isolate 97-804 (from Korea; pattern I3); lane M, lambda ladder PFGE marker.

    Techniques Used: Isolation, Agarose Gel Electrophoresis, Marker

    PFGE patterns of O3:K6 strains of V. parahaemolyticus isolated before 1996. (A) Lane 1, isolate AQ3810 (traveler from Singapore; pattern R); lane 2, isolate AQ4019 (traveler from Singapore; pattern A3); lane 3, isolate AQ4037 (traveler from Maldive Islands; pattern A3); lane 4, isolate AQ4093 (traveler from Maldive Islands; pattern A3); lane 5, isolate AQ4133 (traveler from Hong Kong, pattern A3); lane 6, isolate AQ4235 (traveler from Thailand; pattern A1); lane 7, isolate AQ4299 (traveler from Thailand; pattern A1); lane 8, isolate AQ4644 (traveler from Hong Kong; pattern A3); lane M, lambda ladder PFGE marker, 48.5 kb at the bottom with an increment of 48.5 kb. (B) Lane 1, isolate AQ4733 (traveler from Singapore; pattern A2); lane 2, isolate AQ4853 (traveler from Hong Kong; pattern A3); lane M, lambda ladder PFGE marker.
    Figure Legend Snippet: PFGE patterns of O3:K6 strains of V. parahaemolyticus isolated before 1996. (A) Lane 1, isolate AQ3810 (traveler from Singapore; pattern R); lane 2, isolate AQ4019 (traveler from Singapore; pattern A3); lane 3, isolate AQ4037 (traveler from Maldive Islands; pattern A3); lane 4, isolate AQ4093 (traveler from Maldive Islands; pattern A3); lane 5, isolate AQ4133 (traveler from Hong Kong, pattern A3); lane 6, isolate AQ4235 (traveler from Thailand; pattern A1); lane 7, isolate AQ4299 (traveler from Thailand; pattern A1); lane 8, isolate AQ4644 (traveler from Hong Kong; pattern A3); lane M, lambda ladder PFGE marker, 48.5 kb at the bottom with an increment of 48.5 kb. (B) Lane 1, isolate AQ4733 (traveler from Singapore; pattern A2); lane 2, isolate AQ4853 (traveler from Hong Kong; pattern A3); lane M, lambda ladder PFGE marker.

    Techniques Used: Isolation, Marker

    4) Product Images from "Adventures in the Enormous: A 1.8 Million Clone BAC Library for the 21.7 Gb Genome of Loblolly Pine"

    Article Title: Adventures in the Enormous: A 1.8 Million Clone BAC Library for the 21.7 Gb Genome of Loblolly Pine

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0016214

    Inserts of LP 7-56 BAC clones. Insert size distribution of clones from (A) plates 1-2650, (B) plates 2651-4752, and (C) the library as a whole. (D) A typical agarose pulsed-field gel showing Not I digests of clones from the latter half of the library. The New England Biolabs PFGE Lambda Ladder is in the lane at the far left. A 7.5 kb vector band is visible at the bottom of each sample lane.
    Figure Legend Snippet: Inserts of LP 7-56 BAC clones. Insert size distribution of clones from (A) plates 1-2650, (B) plates 2651-4752, and (C) the library as a whole. (D) A typical agarose pulsed-field gel showing Not I digests of clones from the latter half of the library. The New England Biolabs PFGE Lambda Ladder is in the lane at the far left. A 7.5 kb vector band is visible at the bottom of each sample lane.

    Techniques Used: BAC Assay, Clone Assay, Pulsed-Field Gel, Plasmid Preparation

    5) Product Images from "Dissemination and genetic analysis of the stealthy vanB gene clusters of Enterococcus faecium clinical isolates in Japan"

    Article Title: Dissemination and genetic analysis of the stealthy vanB gene clusters of Enterococcus faecium clinical isolates in Japan

    Journal: BMC Microbiology

    doi: 10.1186/s12866-018-1342-1

    PFGE profiles and dendrogram of the VAN susceptible VanB-type E. faecium isolates. Bacterial DNAs were digested with Sma I and separated by pulsed-field gel electrophoresis (PFGE). The genetic relatedness was analyzed using the Dice coefficient and the dendrogram was constructed with the clustering algorithm of Unweighted Pair-Group Method with an Arithmetic Mean (UPGMA) using FP Quest Software (Bio-Rad). The optimization and the tolerance were 1 and 1.5%, respectively. Major clusters and subclusters of the isolates were delineated with 85 and 90% similarity cutoff values for PFGE as indicated by the vertical solid line and dotted line, respectively. A lambda PFG Ladder (New England BioLabs, MA) was used as the Molecular Marker (MM)
    Figure Legend Snippet: PFGE profiles and dendrogram of the VAN susceptible VanB-type E. faecium isolates. Bacterial DNAs were digested with Sma I and separated by pulsed-field gel electrophoresis (PFGE). The genetic relatedness was analyzed using the Dice coefficient and the dendrogram was constructed with the clustering algorithm of Unweighted Pair-Group Method with an Arithmetic Mean (UPGMA) using FP Quest Software (Bio-Rad). The optimization and the tolerance were 1 and 1.5%, respectively. Major clusters and subclusters of the isolates were delineated with 85 and 90% similarity cutoff values for PFGE as indicated by the vertical solid line and dotted line, respectively. A lambda PFG Ladder (New England BioLabs, MA) was used as the Molecular Marker (MM)

    Techniques Used: Pulsed-Field Gel, Electrophoresis, Construct, Software, Marker

    6) Product Images from "Genetic Diversity among Strains of Moraxella catarrhalis: Analysis Using Multiple DNA Probes and a Single-Locus PCR-Restriction Fragment Length Polymorphism Method"

    Article Title: Genetic Diversity among Strains of Moraxella catarrhalis: Analysis Using Multiple DNA Probes and a Single-Locus PCR-Restriction Fragment Length Polymorphism Method

    Journal: Journal of Clinical Microbiology

    doi:

    Examples of patterns produced by Spe I PFGE restriction digestion of M. catarrhalis strains. The strains are from four children with otitis media. Lane M contains the 48.5-kb ladder formed from concatamers of the bacteriophage lambda genome, with the lowest band a monomer. Note the concentration of restriction fragments smaller than 97 kb. Lanes 1 to 8 contain strains NY1a, NY1b, NY2a, NY3a, NY2b, NY3b, NY4a, and NY4b, respectively.
    Figure Legend Snippet: Examples of patterns produced by Spe I PFGE restriction digestion of M. catarrhalis strains. The strains are from four children with otitis media. Lane M contains the 48.5-kb ladder formed from concatamers of the bacteriophage lambda genome, with the lowest band a monomer. Note the concentration of restriction fragments smaller than 97 kb. Lanes 1 to 8 contain strains NY1a, NY1b, NY2a, NY3a, NY2b, NY3b, NY4a, and NY4b, respectively.

    Techniques Used: Produced, Concentration Assay

    7) Product Images from "Molecular Analysis of Isoleucyl-tRNA Synthetase Mutations in Clinical Isolates of Methicillin-Resistant Staphylococcus aureus with Low-Level Mupirocin Resistance"

    Article Title: Molecular Analysis of Isoleucyl-tRNA Synthetase Mutations in Clinical Isolates of Methicillin-Resistant Staphylococcus aureus with Low-Level Mupirocin Resistance

    Journal: Journal of Korean Medical Science

    doi: 10.3346/jkms.2006.21.5.827

    Representative PFGE patterns of Sma I macrorestriction fragments of genomic DNA of MRSA strains with low-level mupirocin resistance isolated from patients in ICUs. Lane 1, λ ladder marker; lane 2 to lane 7 show PFGE patterns A0, A1, B, C, D, and E, respectively.
    Figure Legend Snippet: Representative PFGE patterns of Sma I macrorestriction fragments of genomic DNA of MRSA strains with low-level mupirocin resistance isolated from patients in ICUs. Lane 1, λ ladder marker; lane 2 to lane 7 show PFGE patterns A0, A1, B, C, D, and E, respectively.

    Techniques Used: Isolation, Marker

    Related Articles

    Clone Assay:

    Article Title: A BAC-based physical map of the Nile tilapia genome
    Article Snippet: We fingerprinted clones from the two libraries with the largest average insert size (Table ). .. Insert sizes of 200 BACs from each library were determined by NotI digestion and comparison to a lambda PFG ladder (New England Biolabs, Beverly MA).

    Centrifugation:

    Article Title: Linked read technology for assembling large complex and polyploid genomes
    Article Snippet: The isopropanol was decanted, and the remaining pellet washed with 3 mL of 70% ethanol, followed by centrifugation at 2000 x g for 5 min. After decanting the ethanol and air drying, the pellet was re-suspended in 200 uL of DNA Hydration Solution. .. The extracted DNA molecules were visualized via pulsed field gel electrophoresis on a CHEF-DR II [Bio-Rad (Hercules, CA)] instrument run at 6 V/cm with a 0.1- to 40-s pulse time for 16 h. Sizes were determined via comparison to the Lambda PFG Ladder (New England Biolabs (Ipswich, MA) No. N0341S) (Additional file : Figure S1).

    Lambda DNA Preparation:

    Article Title: Isolation, Identification, Prevalence, and Genetic Diversity of Bacillus cereus Group Bacteria From Different Foodstuffs in Tunisia
    Article Snippet: .. Three deposits of lambda ladder PFGE marker (lambda DNA cI857 ind 1 Sam7, GelSyringe™, New England Biolabs, UK) were included on each g el. .. Following electrophoresis, the gel was stained with 1 μg/ml ethidium bromide, washed with deioniezd water, and visualized with an UV transilluminator (Bioblock, Illkirch, France).

    Construct:

    Article Title: A BAC-based physical map of the Nile tilapia genome
    Article Snippet: All four libraries were constructed from the sperm of a single male (#00-0135-EA1B) from a strain originating from Lake Manzallah, Egypt and maintained at the University of Stirling, UK. .. Insert sizes of 200 BACs from each library were determined by NotI digestion and comparison to a lambda PFG ladder (New England Biolabs, Beverly MA).

    Electrophoresis:

    Article Title: Isolation, Identification, Prevalence, and Genetic Diversity of Bacillus cereus Group Bacteria From Different Foodstuffs in Tunisia
    Article Snippet: To perform enzymatic digestion, blocks were dialyzed in 600 μl enzyme restriction buffer for 1 h at 4°C and then incubated in 300 μl SmaI solution (New England Biolabs, Hitchin, UK) (20 U in its buffer) at 25°C during 4 h. The sectioned agarose blocks were then loaded into the wells of an ultra-pure agarose gel (Gibco BRL, Paisley, Ecosse) (1.5% m/v in 0.5 X TBE buffer), and subjected to transverse alternative field electrophoresis in a CHEF-DR® II apparatus (Biorad, Hercules CA, USA), using the following parameters: 200 V, 2 s of initial switch time, 20 s of final switch time, and 18–24 h of migration at 14°C. .. Three deposits of lambda ladder PFGE marker (lambda DNA cI857 ind 1 Sam7, GelSyringe™, New England Biolabs, UK) were included on each g el.

    Article Title: A toolkit for rapid CRISPR-SpCas9 assisted construction of hexose-transport-deficient Saccharomyces cerevisiae strains
    Article Snippet: Paragraph title: CHEF electrophoresis ... The agarose plugs were placed in a 1% megabase agarose gel in 0.5x TBE buffer (Thermo Fisher Scientific), together with the Lambda PFG Ladder (New England Biolabs).

    Article Title: Discovery of Alternative Producers of the Enediyne Antitumor Antibiotic C-1027 with High Titers
    Article Snippet: Lambda PFG Ladder (New England Biolabs) was used as the size standard. .. Electrophoresis was run at 4.5 V/cm using a ramped switch time from 5 to 120 s for 48 h at 15 °C.

    Article Title: Genetic Diversity among Strains of Moraxella catarrhalis: Analysis Using Multiple DNA Probes and a Single-Locus PCR-Restriction Fragment Length Polymorphism Method
    Article Snippet: Lambda concatamers (Lambda Ladder PFG marker; New England Biolabs) were used as a molecular weight standard. .. Electrophoresis was performed with the contour-clamped homogeneous gel electrophoresis device (CHEF DR II; Bio-Rad, Richmond, Calif.).

    Article Title: A toolkit for rapid CRISPR-SpCas9 assisted construction of hexose-transport-deficient Saccharomyces cerevisiae strains
    Article Snippet: Paragraph title: CHEF electrophoresis ... The agarose plugs were placed in a 1% megabase agarose gel in 0.5x TBE buffer (Thermo Fisher Scientific), together with the Lambda PFG Ladder (New England Biolabs).

    Article Title: The chromatin structuring protein HMGA2 influences human subtelomere stability and cancer chemosensitivity
    Article Snippet: Paragraph title: Pulsed Field Gel Electrophoresis (PFGE) ... Lambda PFG ladder (48.5–1018 kb; NEB) was used as a molecular size marker.

    Article Title: Linked read technology for assembling large complex and polyploid genomes
    Article Snippet: .. The extracted DNA molecules were visualized via pulsed field gel electrophoresis on a CHEF-DR II [Bio-Rad (Hercules, CA)] instrument run at 6 V/cm with a 0.1- to 40-s pulse time for 16 h. Sizes were determined via comparison to the Lambda PFG Ladder (New England Biolabs (Ipswich, MA) No. N0341S) (Additional file : Figure S1). ..

    Incubation:

    Article Title: Isolation, Identification, Prevalence, and Genetic Diversity of Bacillus cereus Group Bacteria From Different Foodstuffs in Tunisia
    Article Snippet: To perform enzymatic digestion, blocks were dialyzed in 600 μl enzyme restriction buffer for 1 h at 4°C and then incubated in 300 μl SmaI solution (New England Biolabs, Hitchin, UK) (20 U in its buffer) at 25°C during 4 h. The sectioned agarose blocks were then loaded into the wells of an ultra-pure agarose gel (Gibco BRL, Paisley, Ecosse) (1.5% m/v in 0.5 X TBE buffer), and subjected to transverse alternative field electrophoresis in a CHEF-DR® II apparatus (Biorad, Hercules CA, USA), using the following parameters: 200 V, 2 s of initial switch time, 20 s of final switch time, and 18–24 h of migration at 14°C. .. Three deposits of lambda ladder PFGE marker (lambda DNA cI857 ind 1 Sam7, GelSyringe™, New England Biolabs, UK) were included on each g el.

    Article Title: Discovery of Alternative Producers of the Enediyne Antitumor Antibiotic C-1027 with High Titers
    Article Snippet: After embedding the mycelia in low melting point agarose, the blocks were incubated at 37 °C for 90 min in lysozyme solution (40 min for S . sp. CB02366). .. Lambda PFG Ladder (New England Biolabs) was used as the size standard.

    Article Title: Genetic Diversity among Strains of Moraxella catarrhalis: Analysis Using Multiple DNA Probes and a Single-Locus PCR-Restriction Fragment Length Polymorphism Method
    Article Snippet: For restriction endonuclease digestion, an 80-μl agarose plug containing DNA was placed into restriction enzyme buffer containing 100 mg of acetylated bovine serum albumin and 12 U of Spe I (New England Biolabs, Beverly, Mass.) and incubated overnight at 37°C. .. Lambda concatamers (Lambda Ladder PFG marker; New England Biolabs) were used as a molecular weight standard.

    Article Title: Oncofetal HMGA2 attenuates genotoxic damage induced by topoisomerase II target compounds through the regulation of local DNA topology
    Article Snippet: Harvested cells were embedded in 2% low melting agarose (Sigma) plugs followed by incubation in lysis buffer (0.2% sodium deoxycholate, 1% sodium lauroyl sarcosine, 100 mm EDTA, and 1 mg·mL−1 proteinase K) at 50 °C for 24 h. Plugs were washed four times in TE buffer for 1 h each and electrophoresed through 1% megabase agarose (Bio‐Rad) on CHEF DR II (Bio‐Rad). .. Lambda PFG ladder (NEB, Ipswich, MA, USA) that ranges from 48.5 to 1018 kb was used as a marker.

    Article Title: The chromatin structuring protein HMGA2 influences human subtelomere stability and cancer chemosensitivity
    Article Snippet: After solidification, plugs were incubated in lysis buffer (100 mM EDTA, 0.2% (w/v) sodium deoxycholate, 1% (w/v) sodium lauroyl sarcosine and 1 mg/ml proteinase K; Promega) at 50°C for 24 h and washed four times in TE buffer (20 mM Tris, pH 8, 50 mM EDTA) for 1 h each. .. Lambda PFG ladder (48.5–1018 kb; NEB) was used as a molecular size marker.

    Article Title: Linked read technology for assembling large complex and polyploid genomes
    Article Snippet: RNA was removed by adding 15 uL of RNase A Solution, followed by incubation at 37 °C for 15 min. .. The extracted DNA molecules were visualized via pulsed field gel electrophoresis on a CHEF-DR II [Bio-Rad (Hercules, CA)] instrument run at 6 V/cm with a 0.1- to 40-s pulse time for 16 h. Sizes were determined via comparison to the Lambda PFG Ladder (New England Biolabs (Ipswich, MA) No. N0341S) (Additional file : Figure S1).

    Modification:

    Article Title: Linked read technology for assembling large complex and polyploid genomes
    Article Snippet: DNA was extracted from the resulting tissue using the Gentra Puregene Cell Kit [Qiagen (Valencia, CA), No. 158745] following the manufacturer’s protocol modified as follows to reduce the risk of shearing long DNA molecules. .. The extracted DNA molecules were visualized via pulsed field gel electrophoresis on a CHEF-DR II [Bio-Rad (Hercules, CA)] instrument run at 6 V/cm with a 0.1- to 40-s pulse time for 16 h. Sizes were determined via comparison to the Lambda PFG Ladder (New England Biolabs (Ipswich, MA) No. N0341S) (Additional file : Figure S1).

    Hybridization:

    Article Title: Discovery of Alternative Producers of the Enediyne Antitumor Antibiotic C-1027 with High Titers
    Article Snippet: Lambda PFG Ladder (New England Biolabs) was used as the size standard. .. Southern analysis for the C-1027 BGCs was performed with the DIG Easy hybridization kit from Roche according to the manufacturer’s instructions.

    Southern Blot:

    Article Title: Oncofetal HMGA2 attenuates genotoxic damage induced by topoisomerase II target compounds through the regulation of local DNA topology
    Article Snippet: Paragraph title: Pulsed‐field gel electrophoresis (PFGE) and Southern blotting ... Lambda PFG ladder (NEB, Ipswich, MA, USA) that ranges from 48.5 to 1018 kb was used as a marker.

    Cell Culture:

    Article Title: Discovery of Alternative Producers of the Enediyne Antitumor Antibiotic C-1027 with High Titers
    Article Snippet: Spores of the Streptomyces strains were collected from ISP4 agar plates and inoculated into 250 mL baffled flasks containing 50 mL of yeast extract−malt extract (YEME) liquid medium ( S . sp. CB02366 was cultured in TSB liquid medium due to the poor growth in the YEME liquid medium). .. Lambda PFG Ladder (New England Biolabs) was used as the size standard.

    Sequencing:

    Article Title: Linked read technology for assembling large complex and polyploid genomes
    Article Snippet: Paragraph title: High molecular weight DNA preparation, sequencing, and assembly ... The extracted DNA molecules were visualized via pulsed field gel electrophoresis on a CHEF-DR II [Bio-Rad (Hercules, CA)] instrument run at 6 V/cm with a 0.1- to 40-s pulse time for 16 h. Sizes were determined via comparison to the Lambda PFG Ladder (New England Biolabs (Ipswich, MA) No. N0341S) (Additional file : Figure S1).

    Pulsed-Field Gel:

    Article Title: Dissemination and genetic analysis of the stealthy vanB gene clusters of Enterococcus faecium clinical isolates in Japan
    Article Snippet: Paragraph title: Pulsed-field gel electrophoresis (PFGE) analysis and dendrogram ... A lambda PFG Ladder (New England BioLabs, MA) was used as the Molecular Marker (MM).

    Article Title: Isolation, Identification, Prevalence, and Genetic Diversity of Bacillus cereus Group Bacteria From Different Foodstuffs in Tunisia
    Article Snippet: Paragraph title: Pulsed-field gel electrophoresis ... Three deposits of lambda ladder PFGE marker (lambda DNA cI857 ind 1 Sam7, GelSyringe™, New England Biolabs, UK) were included on each g el.

    Article Title: Acquisition and transfer of antibiotic resistance genes in association with conjugative plasmid or class 1 integrons of Acinetobacter baumannii
    Article Snippet: Paragraph title: S1-nuclease pulsed-field gel electrophoresis (S1-PFGE) and characterization of plasmids obtained from successful transconjugants ... Linearized plasmid sizes were determined with Lambda PFG Ladder ( New England Biolabs, Frankfurt, Germany ) .

    Article Title: Oncofetal HMGA2 attenuates genotoxic damage induced by topoisomerase II target compounds through the regulation of local DNA topology
    Article Snippet: Paragraph title: Pulsed‐field gel electrophoresis (PFGE) and Southern blotting ... Lambda PFG ladder (NEB, Ipswich, MA, USA) that ranges from 48.5 to 1018 kb was used as a marker.

    Article Title: The chromatin structuring protein HMGA2 influences human subtelomere stability and cancer chemosensitivity
    Article Snippet: Paragraph title: Pulsed Field Gel Electrophoresis (PFGE) ... Lambda PFG ladder (48.5–1018 kb; NEB) was used as a molecular size marker.

    Article Title: Linked read technology for assembling large complex and polyploid genomes
    Article Snippet: .. The extracted DNA molecules were visualized via pulsed field gel electrophoresis on a CHEF-DR II [Bio-Rad (Hercules, CA)] instrument run at 6 V/cm with a 0.1- to 40-s pulse time for 16 h. Sizes were determined via comparison to the Lambda PFG Ladder (New England Biolabs (Ipswich, MA) No. N0341S) (Additional file : Figure S1). ..

    Migration:

    Article Title: Isolation, Identification, Prevalence, and Genetic Diversity of Bacillus cereus Group Bacteria From Different Foodstuffs in Tunisia
    Article Snippet: To perform enzymatic digestion, blocks were dialyzed in 600 μl enzyme restriction buffer for 1 h at 4°C and then incubated in 300 μl SmaI solution (New England Biolabs, Hitchin, UK) (20 U in its buffer) at 25°C during 4 h. The sectioned agarose blocks were then loaded into the wells of an ultra-pure agarose gel (Gibco BRL, Paisley, Ecosse) (1.5% m/v in 0.5 X TBE buffer), and subjected to transverse alternative field electrophoresis in a CHEF-DR® II apparatus (Biorad, Hercules CA, USA), using the following parameters: 200 V, 2 s of initial switch time, 20 s of final switch time, and 18–24 h of migration at 14°C. .. Three deposits of lambda ladder PFGE marker (lambda DNA cI857 ind 1 Sam7, GelSyringe™, New England Biolabs, UK) were included on each g el.

    Nucleic Acid Electrophoresis:

    Article Title: Genetic Diversity among Strains of Moraxella catarrhalis: Analysis Using Multiple DNA Probes and a Single-Locus PCR-Restriction Fragment Length Polymorphism Method
    Article Snippet: Lambda concatamers (Lambda Ladder PFG marker; New England Biolabs) were used as a molecular weight standard. .. Electrophoresis was performed with the contour-clamped homogeneous gel electrophoresis device (CHEF DR II; Bio-Rad, Richmond, Calif.).

    Purification:

    Article Title: Acquisition and transfer of antibiotic resistance genes in association with conjugative plasmid or class 1 integrons of Acinetobacter baumannii
    Article Snippet: Linearized plasmid sizes were determined with Lambda PFG Ladder ( New England Biolabs, Frankfurt, Germany ) . .. Afterwards, the genomic and plasmid DNA of donor and transconjugant strains were cut and purified using GeneJET PCR Purification Kit (Thermo Fisher Scientific, illkirch, France).

    Article Title: Genetic Diversity among Strains of Moraxella catarrhalis: Analysis Using Multiple DNA Probes and a Single-Locus PCR-Restriction Fragment Length Polymorphism Method
    Article Snippet: M. catarrhalis strains were grown overnight at 37°C in brain heart infusion broth, and DNA was purified as described previously ( ). .. Lambda concatamers (Lambda Ladder PFG marker; New England Biolabs) were used as a molecular weight standard.

    Polymerase Chain Reaction:

    Article Title: Acquisition and transfer of antibiotic resistance genes in association with conjugative plasmid or class 1 integrons of Acinetobacter baumannii
    Article Snippet: Linearized plasmid sizes were determined with Lambda PFG Ladder ( New England Biolabs, Frankfurt, Germany ) . .. Afterwards, the genomic and plasmid DNA of donor and transconjugant strains were cut and purified using GeneJET PCR Purification Kit (Thermo Fisher Scientific, illkirch, France).

    Lysis:

    Article Title: Oncofetal HMGA2 attenuates genotoxic damage induced by topoisomerase II target compounds through the regulation of local DNA topology
    Article Snippet: Harvested cells were embedded in 2% low melting agarose (Sigma) plugs followed by incubation in lysis buffer (0.2% sodium deoxycholate, 1% sodium lauroyl sarcosine, 100 mm EDTA, and 1 mg·mL−1 proteinase K) at 50 °C for 24 h. Plugs were washed four times in TE buffer for 1 h each and electrophoresed through 1% megabase agarose (Bio‐Rad) on CHEF DR II (Bio‐Rad). .. Lambda PFG ladder (NEB, Ipswich, MA, USA) that ranges from 48.5 to 1018 kb was used as a marker.

    Article Title: The chromatin structuring protein HMGA2 influences human subtelomere stability and cancer chemosensitivity
    Article Snippet: After solidification, plugs were incubated in lysis buffer (100 mM EDTA, 0.2% (w/v) sodium deoxycholate, 1% (w/v) sodium lauroyl sarcosine and 1 mg/ml proteinase K; Promega) at 50°C for 24 h and washed four times in TE buffer (20 mM Tris, pH 8, 50 mM EDTA) for 1 h each. .. Lambda PFG ladder (48.5–1018 kb; NEB) was used as a molecular size marker.

    Article Title: Linked read technology for assembling large complex and polyploid genomes
    Article Snippet: Briefly, 3 mL of Cell Lysis Solution were mixed with 100 mg of ground tissue, followed by a 60-min incubation at 65 °C. .. The extracted DNA molecules were visualized via pulsed field gel electrophoresis on a CHEF-DR II [Bio-Rad (Hercules, CA)] instrument run at 6 V/cm with a 0.1- to 40-s pulse time for 16 h. Sizes were determined via comparison to the Lambda PFG Ladder (New England Biolabs (Ipswich, MA) No. N0341S) (Additional file : Figure S1).

    Chromatin Immunoprecipitation:

    Article Title: Linked read technology for assembling large complex and polyploid genomes
    Article Snippet: The extracted DNA molecules were visualized via pulsed field gel electrophoresis on a CHEF-DR II [Bio-Rad (Hercules, CA)] instrument run at 6 V/cm with a 0.1- to 40-s pulse time for 16 h. Sizes were determined via comparison to the Lambda PFG Ladder (New England Biolabs (Ipswich, MA) No. N0341S) (Additional file : Figure S1). .. A (CG00022 RevA) using the Chromium Genome Chip Kit [10× Genomics (Pleasanton, CA) No. PN-120216] and the Genome Library, Gel Bead & Multiplex V1 Kit [10× Genomics (Pleasanton, CA) No. PN-120229] with the modification of using 0.9 ng of genomic DNA input (~ 355 genome equivalents to maize).

    Plasmid Preparation:

    Article Title: Acquisition and transfer of antibiotic resistance genes in association with conjugative plasmid or class 1 integrons of Acinetobacter baumannii
    Article Snippet: .. Linearized plasmid sizes were determined with Lambda PFG Ladder ( New England Biolabs, Frankfurt, Germany ) . .. Afterwards, the genomic and plasmid DNA of donor and transconjugant strains were cut and purified using GeneJET PCR Purification Kit (Thermo Fisher Scientific, illkirch, France).

    Software:

    Article Title: Dissemination and genetic analysis of the stealthy vanB gene clusters of Enterococcus faecium clinical isolates in Japan
    Article Snippet: The genetic relatedness was analyzed using the Dice coefficient and the dendrogram, and was calculated with the clustering algorithm of Unweighted Pair-Group Method with an Arithmetic Mean (UPGMA) using FP Quest Software (Bio-Rad) [ , ]. .. A lambda PFG Ladder (New England BioLabs, MA) was used as the Molecular Marker (MM).

    Article Title: Dissemination and genetic analysis of the stealthy vanB gene clusters of Enterococcus faecium clinical isolates in Japan
    Article Snippet: The genetic relatedness was analyzed using the Dice coefficient and the dendrogram, and was calculated with the clustering algorithm of Unweighted Pair-Group Method with an Arithmetic Mean (UPGMA) using FP Quest Software (Bio-Rad) [ , ]. .. A lambda PFG Ladder (New England BioLabs, MA) was used as the Molecular Marker (MM).

    Article Title: Oncofetal HMGA2 attenuates genotoxic damage induced by topoisomerase II target compounds through the regulation of local DNA topology
    Article Snippet: Lambda PFG ladder (NEB, Ipswich, MA, USA) that ranges from 48.5 to 1018 kb was used as a marker. .. Subsequently, PFGE gels were stained with ethidium bromide, and quantification was performed using imagej software, as described in detail in Figure legends.

    Multiplex Assay:

    Article Title: Acquisition and transfer of antibiotic resistance genes in association with conjugative plasmid or class 1 integrons of Acinetobacter baumannii
    Article Snippet: Linearized plasmid sizes were determined with Lambda PFG Ladder ( New England Biolabs, Frankfurt, Germany ) . .. Detection of antibiotic resistance genes, integrase genes, and plasmid replicon types in purified DNA of transconjugants were performed using monoplex PCR and multiplex PCR methods.

    Article Title: Linked read technology for assembling large complex and polyploid genomes
    Article Snippet: The extracted DNA molecules were visualized via pulsed field gel electrophoresis on a CHEF-DR II [Bio-Rad (Hercules, CA)] instrument run at 6 V/cm with a 0.1- to 40-s pulse time for 16 h. Sizes were determined via comparison to the Lambda PFG Ladder (New England Biolabs (Ipswich, MA) No. N0341S) (Additional file : Figure S1). .. A (CG00022 RevA) using the Chromium Genome Chip Kit [10× Genomics (Pleasanton, CA) No. PN-120216] and the Genome Library, Gel Bead & Multiplex V1 Kit [10× Genomics (Pleasanton, CA) No. PN-120229] with the modification of using 0.9 ng of genomic DNA input (~ 355 genome equivalents to maize).

    Agarose Gel Electrophoresis:

    Article Title: Isolation, Identification, Prevalence, and Genetic Diversity of Bacillus cereus Group Bacteria From Different Foodstuffs in Tunisia
    Article Snippet: To perform enzymatic digestion, blocks were dialyzed in 600 μl enzyme restriction buffer for 1 h at 4°C and then incubated in 300 μl SmaI solution (New England Biolabs, Hitchin, UK) (20 U in its buffer) at 25°C during 4 h. The sectioned agarose blocks were then loaded into the wells of an ultra-pure agarose gel (Gibco BRL, Paisley, Ecosse) (1.5% m/v in 0.5 X TBE buffer), and subjected to transverse alternative field electrophoresis in a CHEF-DR® II apparatus (Biorad, Hercules CA, USA), using the following parameters: 200 V, 2 s of initial switch time, 20 s of final switch time, and 18–24 h of migration at 14°C. .. Three deposits of lambda ladder PFGE marker (lambda DNA cI857 ind 1 Sam7, GelSyringe™, New England Biolabs, UK) were included on each g el.

    Article Title: A toolkit for rapid CRISPR-SpCas9 assisted construction of hexose-transport-deficient Saccharomyces cerevisiae strains
    Article Snippet: .. The agarose plugs were placed in a 1% megabase agarose gel in 0.5x TBE buffer (Thermo Fisher Scientific), together with the Lambda PFG Ladder (New England Biolabs). .. The CHEF-DRIII Pulsed Field Electrophoresis system (Bio-Rad) chilled to 14°C was used with a voltage of 5 V/cm, a pulse angle of 120° and pulse time of 60 s during 28 h followed by a pulse time of 90 s for another 16 h. The CHEF gel was stained in 200 mL 0.5x TBE with 3 μg mL−1 ethidium bromide followed by de-staining in 200 mL 0.5× TBE.

    Article Title: Discovery of Alternative Producers of the Enediyne Antitumor Antibiotic C-1027 with High Titers
    Article Snippet: After treatment with proteinase K solution and the wash steps, the blocks were loaded into the 1% agarose gel containing 100 μ M thiourea. .. Lambda PFG Ladder (New England Biolabs) was used as the size standard.

    Article Title: A toolkit for rapid CRISPR-SpCas9 assisted construction of hexose-transport-deficient Saccharomyces cerevisiae strains
    Article Snippet: .. The agarose plugs were placed in a 1% megabase agarose gel in 0.5x TBE buffer (Thermo Fisher Scientific), together with the Lambda PFG Ladder (New England Biolabs). .. The CHEF-DRIII Pulsed Field Electrophoresis system (Bio-Rad) chilled to 14°C was used with a voltage of 5 V/cm, a pulse angle of 120° and pulse time of 60 s during 28 h followed by a pulse time of 90 s for another 16 h. The CHEF gel was stained in 200 mL 0.5x TBE with 3 μg mL−1 ethidium bromide followed by de-staining in 200 mL 0.5× TBE.

    Molecular Weight:

    Article Title: Genetic Diversity among Strains of Moraxella catarrhalis: Analysis Using Multiple DNA Probes and a Single-Locus PCR-Restriction Fragment Length Polymorphism Method
    Article Snippet: .. Lambda concatamers (Lambda Ladder PFG marker; New England Biolabs) were used as a molecular weight standard. .. Electrophoresis was performed with the contour-clamped homogeneous gel electrophoresis device (CHEF DR II; Bio-Rad, Richmond, Calif.).

    Article Title: Linked read technology for assembling large complex and polyploid genomes
    Article Snippet: Paragraph title: High molecular weight DNA preparation, sequencing, and assembly ... The extracted DNA molecules were visualized via pulsed field gel electrophoresis on a CHEF-DR II [Bio-Rad (Hercules, CA)] instrument run at 6 V/cm with a 0.1- to 40-s pulse time for 16 h. Sizes were determined via comparison to the Lambda PFG Ladder (New England Biolabs (Ipswich, MA) No. N0341S) (Additional file : Figure S1).

    BAC Assay:

    Article Title: A BAC-based physical map of the Nile tilapia genome
    Article Snippet: Paragraph title: Source BAC libraries ... Insert sizes of 200 BACs from each library were determined by NotI digestion and comparison to a lambda PFG ladder (New England Biolabs, Beverly MA).

    Marker:

    Article Title: Dissemination and genetic analysis of the stealthy vanB gene clusters of Enterococcus faecium clinical isolates in Japan
    Article Snippet: .. A lambda PFG Ladder (New England BioLabs, MA) was used as the Molecular Marker (MM). .. Southern transfer and hybridization analysis PFGE analyses with S1 nuclease or I-Ceu I were performed as described above.

    Article Title: Dissemination and genetic analysis of the stealthy vanB gene clusters of Enterococcus faecium clinical isolates in Japan
    Article Snippet: .. A lambda PFG Ladder (New England BioLabs, MA) was used as the Molecular Marker (MM). ..

    Article Title: Isolation, Identification, Prevalence, and Genetic Diversity of Bacillus cereus Group Bacteria From Different Foodstuffs in Tunisia
    Article Snippet: .. Three deposits of lambda ladder PFGE marker (lambda DNA cI857 ind 1 Sam7, GelSyringe™, New England Biolabs, UK) were included on each g el. .. Following electrophoresis, the gel was stained with 1 μg/ml ethidium bromide, washed with deioniezd water, and visualized with an UV transilluminator (Bioblock, Illkirch, France).

    Article Title: Genetic Diversity among Strains of Moraxella catarrhalis: Analysis Using Multiple DNA Probes and a Single-Locus PCR-Restriction Fragment Length Polymorphism Method
    Article Snippet: .. Lambda concatamers (Lambda Ladder PFG marker; New England Biolabs) were used as a molecular weight standard. .. Electrophoresis was performed with the contour-clamped homogeneous gel electrophoresis device (CHEF DR II; Bio-Rad, Richmond, Calif.).

    Article Title: Oncofetal HMGA2 attenuates genotoxic damage induced by topoisomerase II target compounds through the regulation of local DNA topology
    Article Snippet: .. Lambda PFG ladder (NEB, Ipswich, MA, USA) that ranges from 48.5 to 1018 kb was used as a marker. .. Subsequently, PFGE gels were stained with ethidium bromide, and quantification was performed using imagej software, as described in detail in Figure legends.

    Article Title: The chromatin structuring protein HMGA2 influences human subtelomere stability and cancer chemosensitivity
    Article Snippet: .. Lambda PFG ladder (48.5–1018 kb; NEB) was used as a molecular size marker. .. Subsequently, DNA was stained with ethidium bromide and quantification was performed using ImageJ (see figure legends for details).

    Staining:

    Article Title: Isolation, Identification, Prevalence, and Genetic Diversity of Bacillus cereus Group Bacteria From Different Foodstuffs in Tunisia
    Article Snippet: Three deposits of lambda ladder PFGE marker (lambda DNA cI857 ind 1 Sam7, GelSyringe™, New England Biolabs, UK) were included on each g el. .. Following electrophoresis, the gel was stained with 1 μg/ml ethidium bromide, washed with deioniezd water, and visualized with an UV transilluminator (Bioblock, Illkirch, France).

    Article Title: Acquisition and transfer of antibiotic resistance genes in association with conjugative plasmid or class 1 integrons of Acinetobacter baumannii
    Article Snippet: The gels were stained with ethidium bromide (5 μg/ml) for 20 min, and the band patterns were observed under a UV transilluminator. .. Linearized plasmid sizes were determined with Lambda PFG Ladder ( New England Biolabs, Frankfurt, Germany ) .

    Article Title: A toolkit for rapid CRISPR-SpCas9 assisted construction of hexose-transport-deficient Saccharomyces cerevisiae strains
    Article Snippet: The agarose plugs were placed in a 1% megabase agarose gel in 0.5x TBE buffer (Thermo Fisher Scientific), together with the Lambda PFG Ladder (New England Biolabs). .. The CHEF-DRIII Pulsed Field Electrophoresis system (Bio-Rad) chilled to 14°C was used with a voltage of 5 V/cm, a pulse angle of 120° and pulse time of 60 s during 28 h followed by a pulse time of 90 s for another 16 h. The CHEF gel was stained in 200 mL 0.5x TBE with 3 μg mL−1 ethidium bromide followed by de-staining in 200 mL 0.5× TBE.

    Article Title: Discovery of Alternative Producers of the Enediyne Antitumor Antibiotic C-1027 with High Titers
    Article Snippet: Lambda PFG Ladder (New England Biolabs) was used as the size standard. .. After electrophoresis, the gel was stained in 400 mL of deionized water containing 20 μ L of GelRed nucleic acid gel stain for 2−16 h (the staining step was omitted for Southern analysis).

    Article Title: A toolkit for rapid CRISPR-SpCas9 assisted construction of hexose-transport-deficient Saccharomyces cerevisiae strains
    Article Snippet: The agarose plugs were placed in a 1% megabase agarose gel in 0.5x TBE buffer (Thermo Fisher Scientific), together with the Lambda PFG Ladder (New England Biolabs). .. The CHEF-DRIII Pulsed Field Electrophoresis system (Bio-Rad) chilled to 14°C was used with a voltage of 5 V/cm, a pulse angle of 120° and pulse time of 60 s during 28 h followed by a pulse time of 90 s for another 16 h. The CHEF gel was stained in 200 mL 0.5x TBE with 3 μg mL−1 ethidium bromide followed by de-staining in 200 mL 0.5× TBE.

    Article Title: Oncofetal HMGA2 attenuates genotoxic damage induced by topoisomerase II target compounds through the regulation of local DNA topology
    Article Snippet: Lambda PFG ladder (NEB, Ipswich, MA, USA) that ranges from 48.5 to 1018 kb was used as a marker. .. Subsequently, PFGE gels were stained with ethidium bromide, and quantification was performed using imagej software, as described in detail in Figure legends.

    Article Title: The chromatin structuring protein HMGA2 influences human subtelomere stability and cancer chemosensitivity
    Article Snippet: Lambda PFG ladder (48.5–1018 kb; NEB) was used as a molecular size marker. .. Subsequently, DNA was stained with ethidium bromide and quantification was performed using ImageJ (see figure legends for details).

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    New England Biolabs lambda ladder pfge
    Detection of plasmids in 25 staphylococcal clinical isolates by <t>PFGE.</t> Large plasmids ( > 30 kb) were analyzed by PFGE after digestion with nuclease S1 at 37°C for 45 min. Lanes 1–25: clinical isolates; lane number corresponds to the number of the isolate. Lane C: positive control, plasmid pSK41 (46.4 kb) extracted from SK5428 strain. Lane M: <t>Lambda</t> Ladder PFGE molecular size marker. Arrows point to detected plasmids.
    Lambda Ladder Pfge, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Detection of plasmids in 25 staphylococcal clinical isolates by PFGE. Large plasmids ( > 30 kb) were analyzed by PFGE after digestion with nuclease S1 at 37°C for 45 min. Lanes 1–25: clinical isolates; lane number corresponds to the number of the isolate. Lane C: positive control, plasmid pSK41 (46.4 kb) extracted from SK5428 strain. Lane M: Lambda Ladder PFGE molecular size marker. Arrows point to detected plasmids.

    Journal: Frontiers in Microbiology

    Article Title: Biofilm-Forming Clinical Staphylococcus Isolates Harbor Horizontal Transfer and Antibiotic Resistance Genes

    doi: 10.3389/fmicb.2017.02018

    Figure Lengend Snippet: Detection of plasmids in 25 staphylococcal clinical isolates by PFGE. Large plasmids ( > 30 kb) were analyzed by PFGE after digestion with nuclease S1 at 37°C for 45 min. Lanes 1–25: clinical isolates; lane number corresponds to the number of the isolate. Lane C: positive control, plasmid pSK41 (46.4 kb) extracted from SK5428 strain. Lane M: Lambda Ladder PFGE molecular size marker. Arrows point to detected plasmids.

    Article Snippet: The reaction was stopped by transferring the slices to 1 ml of TE buffer for 1 h. Digested slices were applied to wells in 1% (w/v) Pulsed Field Certified Agarose (BioRad) prepared in 0.5 × TBE buffer [45 mM Tris (pH 8), 45 mM boric acid, 1 mM EDTA] and run in CHEF-DR® III System (BioRad) at 6 V/cm, a field angle of 120°, and switch times of 5 to 35 s for 22 h. Lambda Ladder PFGE (New England Biolabs, Ispwich, U.S) was used as molecular size marker and pSK41 plasmid (46.4 kb) was used as positive control.

    Techniques: Positive Control, Plasmid Preparation, Marker

    C-1027 BGCs residing on giant plasmids of varying size in the five C-1027 producers. (A) PFGE analysis of the five C-1027 producers showing the existence of giant plasmids of varying sizes. (B) Southern analysis revealing that the C-1027 BGCs all reside on giant plasmids in the five producers. M, Lambda PFG ladder (NEB); lane 1, S. globisporus wild-type; lane 2, S. globisporus AF40; lane 3, CB00657; lane 4, CB02329; lane 5, CB02366; and lane 6, CB03608.

    Journal: Journal of natural products

    Article Title: Discovery of Alternative Producers of the Enediyne Antitumor Antibiotic C-1027 with High Titers

    doi: 10.1021/acs.jnatprod.7b01013

    Figure Lengend Snippet: C-1027 BGCs residing on giant plasmids of varying size in the five C-1027 producers. (A) PFGE analysis of the five C-1027 producers showing the existence of giant plasmids of varying sizes. (B) Southern analysis revealing that the C-1027 BGCs all reside on giant plasmids in the five producers. M, Lambda PFG ladder (NEB); lane 1, S. globisporus wild-type; lane 2, S. globisporus AF40; lane 3, CB00657; lane 4, CB02329; lane 5, CB02366; and lane 6, CB03608.

    Article Snippet: Lambda PFG Ladder (New England Biolabs) was used as the size standard.

    Techniques:

    PFGE patterns of recently isolated O3:K6 strains of V. parahaemolyticus . Conditions for PFGE were 1% agarose gel, 0.5× Tris-borate-EDTA buffer, 190 V, pulse time 3 to 80 s, for 22.4 h. Lane 1, isolate 1020 (from Philippines; pattern I5); lane 2, isolate 1021 (from Singapore; pattern I6); lane 3, isolate 1084 (from Taiwan; pattern I7); lane 4, isolate 1104 (from Taiwan; pattern I8); lane 5, isolate 1123 (from Taiwan; pattern I1); lane 6, isolate 1125 (from Taiwan; pattern I1); lane 7, isolate 1139 (from Taiwan; pattern I4); lane 8, isolate 1154 (from Taiwan; pattern I2); lane 9, isolate 97-804 (from Korea; pattern I3); lane M, lambda ladder PFGE marker.

    Journal: Applied and Environmental Microbiology

    Article Title: Characteristics of Vibrio parahaemolyticus O3:K6 from Asia

    doi:

    Figure Lengend Snippet: PFGE patterns of recently isolated O3:K6 strains of V. parahaemolyticus . Conditions for PFGE were 1% agarose gel, 0.5× Tris-borate-EDTA buffer, 190 V, pulse time 3 to 80 s, for 22.4 h. Lane 1, isolate 1020 (from Philippines; pattern I5); lane 2, isolate 1021 (from Singapore; pattern I6); lane 3, isolate 1084 (from Taiwan; pattern I7); lane 4, isolate 1104 (from Taiwan; pattern I8); lane 5, isolate 1123 (from Taiwan; pattern I1); lane 6, isolate 1125 (from Taiwan; pattern I1); lane 7, isolate 1139 (from Taiwan; pattern I4); lane 8, isolate 1154 (from Taiwan; pattern I2); lane 9, isolate 97-804 (from Korea; pattern I3); lane M, lambda ladder PFGE marker.

    Article Snippet: A lambda ladder PFGE marker (New England BioLabs) was used to mark molecular size.

    Techniques: Isolation, Agarose Gel Electrophoresis, Marker

    PFGE patterns of O3:K6 strains of V. parahaemolyticus isolated before 1996. (A) Lane 1, isolate AQ3810 (traveler from Singapore; pattern R); lane 2, isolate AQ4019 (traveler from Singapore; pattern A3); lane 3, isolate AQ4037 (traveler from Maldive Islands; pattern A3); lane 4, isolate AQ4093 (traveler from Maldive Islands; pattern A3); lane 5, isolate AQ4133 (traveler from Hong Kong, pattern A3); lane 6, isolate AQ4235 (traveler from Thailand; pattern A1); lane 7, isolate AQ4299 (traveler from Thailand; pattern A1); lane 8, isolate AQ4644 (traveler from Hong Kong; pattern A3); lane M, lambda ladder PFGE marker, 48.5 kb at the bottom with an increment of 48.5 kb. (B) Lane 1, isolate AQ4733 (traveler from Singapore; pattern A2); lane 2, isolate AQ4853 (traveler from Hong Kong; pattern A3); lane M, lambda ladder PFGE marker.

    Journal: Applied and Environmental Microbiology

    Article Title: Characteristics of Vibrio parahaemolyticus O3:K6 from Asia

    doi:

    Figure Lengend Snippet: PFGE patterns of O3:K6 strains of V. parahaemolyticus isolated before 1996. (A) Lane 1, isolate AQ3810 (traveler from Singapore; pattern R); lane 2, isolate AQ4019 (traveler from Singapore; pattern A3); lane 3, isolate AQ4037 (traveler from Maldive Islands; pattern A3); lane 4, isolate AQ4093 (traveler from Maldive Islands; pattern A3); lane 5, isolate AQ4133 (traveler from Hong Kong, pattern A3); lane 6, isolate AQ4235 (traveler from Thailand; pattern A1); lane 7, isolate AQ4299 (traveler from Thailand; pattern A1); lane 8, isolate AQ4644 (traveler from Hong Kong; pattern A3); lane M, lambda ladder PFGE marker, 48.5 kb at the bottom with an increment of 48.5 kb. (B) Lane 1, isolate AQ4733 (traveler from Singapore; pattern A2); lane 2, isolate AQ4853 (traveler from Hong Kong; pattern A3); lane M, lambda ladder PFGE marker.

    Article Snippet: A lambda ladder PFGE marker (New England BioLabs) was used to mark molecular size.

    Techniques: Isolation, Marker

    Inserts of LP 7-56 BAC clones. Insert size distribution of clones from (A) plates 1-2650, (B) plates 2651-4752, and (C) the library as a whole. (D) A typical agarose pulsed-field gel showing Not I digests of clones from the latter half of the library. The New England Biolabs PFGE Lambda Ladder is in the lane at the far left. A 7.5 kb vector band is visible at the bottom of each sample lane.

    Journal: PLoS ONE

    Article Title: Adventures in the Enormous: A 1.8 Million Clone BAC Library for the 21.7 Gb Genome of Loblolly Pine

    doi: 10.1371/journal.pone.0016214

    Figure Lengend Snippet: Inserts of LP 7-56 BAC clones. Insert size distribution of clones from (A) plates 1-2650, (B) plates 2651-4752, and (C) the library as a whole. (D) A typical agarose pulsed-field gel showing Not I digests of clones from the latter half of the library. The New England Biolabs PFGE Lambda Ladder is in the lane at the far left. A 7.5 kb vector band is visible at the bottom of each sample lane.

    Article Snippet: The New England Biolabs Lambda PFGE ladder was used as a standard when estimating the sizes of inserts.

    Techniques: BAC Assay, Clone Assay, Pulsed-Field Gel, Plasmid Preparation