lambda ladder pfge  (New England Biolabs)


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    Name:
    Lambda PFG Ladder
    Description:
    Lambda PFG Ladder 50 gel lanes
    Catalog Number:
    n0341s
    Price:
    156
    Size:
    50 gel lanes
    Category:
    DNA Ladders
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    Structured Review

    New England Biolabs lambda ladder pfge
    Lambda PFG Ladder
    Lambda PFG Ladder 50 gel lanes
    https://www.bioz.com/result/lambda ladder pfge/product/New England Biolabs
    Average 96 stars, based on 80 article reviews
    Price from $9.99 to $1999.99
    lambda ladder pfge - by Bioz Stars, 2020-07
    96/100 stars

    Images

    1) Product Images from "Biofilm-Forming Clinical Staphylococcus Isolates Harbor Horizontal Transfer and Antibiotic Resistance Genes"

    Article Title: Biofilm-Forming Clinical Staphylococcus Isolates Harbor Horizontal Transfer and Antibiotic Resistance Genes

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2017.02018

    Detection of plasmids in 25 staphylococcal clinical isolates by PFGE. Large plasmids ( > 30 kb) were analyzed by PFGE after digestion with nuclease S1 at 37°C for 45 min. Lanes 1–25: clinical isolates; lane number corresponds to the number of the isolate. Lane C: positive control, plasmid pSK41 (46.4 kb) extracted from SK5428 strain. Lane M: Lambda Ladder PFGE molecular size marker. Arrows point to detected plasmids.
    Figure Legend Snippet: Detection of plasmids in 25 staphylococcal clinical isolates by PFGE. Large plasmids ( > 30 kb) were analyzed by PFGE after digestion with nuclease S1 at 37°C for 45 min. Lanes 1–25: clinical isolates; lane number corresponds to the number of the isolate. Lane C: positive control, plasmid pSK41 (46.4 kb) extracted from SK5428 strain. Lane M: Lambda Ladder PFGE molecular size marker. Arrows point to detected plasmids.

    Techniques Used: Positive Control, Plasmid Preparation, Marker

    2) Product Images from "Discovery of Alternative Producers of the Enediyne Antitumor Antibiotic C-1027 with High Titers"

    Article Title: Discovery of Alternative Producers of the Enediyne Antitumor Antibiotic C-1027 with High Titers

    Journal: Journal of natural products

    doi: 10.1021/acs.jnatprod.7b01013

    C-1027 BGCs residing on giant plasmids of varying size in the five C-1027 producers. (A) PFGE analysis of the five C-1027 producers showing the existence of giant plasmids of varying sizes. (B) Southern analysis revealing that the C-1027 BGCs all reside on giant plasmids in the five producers. M, Lambda PFG ladder (NEB); lane 1, S. globisporus wild-type; lane 2, S. globisporus AF40; lane 3, CB00657; lane 4, CB02329; lane 5, CB02366; and lane 6, CB03608.
    Figure Legend Snippet: C-1027 BGCs residing on giant plasmids of varying size in the five C-1027 producers. (A) PFGE analysis of the five C-1027 producers showing the existence of giant plasmids of varying sizes. (B) Southern analysis revealing that the C-1027 BGCs all reside on giant plasmids in the five producers. M, Lambda PFG ladder (NEB); lane 1, S. globisporus wild-type; lane 2, S. globisporus AF40; lane 3, CB00657; lane 4, CB02329; lane 5, CB02366; and lane 6, CB03608.

    Techniques Used:

    3) Product Images from "Characteristics of Vibrio parahaemolyticus O3:K6 from Asia"

    Article Title: Characteristics of Vibrio parahaemolyticus O3:K6 from Asia

    Journal: Applied and Environmental Microbiology

    doi:

    PFGE patterns of recently isolated O3:K6 strains of V. parahaemolyticus . Conditions for PFGE were 1% agarose gel, 0.5× Tris-borate-EDTA buffer, 190 V, pulse time 3 to 80 s, for 22.4 h. Lane 1, isolate 1020 (from Philippines; pattern I5); lane 2, isolate 1021 (from Singapore; pattern I6); lane 3, isolate 1084 (from Taiwan; pattern I7); lane 4, isolate 1104 (from Taiwan; pattern I8); lane 5, isolate 1123 (from Taiwan; pattern I1); lane 6, isolate 1125 (from Taiwan; pattern I1); lane 7, isolate 1139 (from Taiwan; pattern I4); lane 8, isolate 1154 (from Taiwan; pattern I2); lane 9, isolate 97-804 (from Korea; pattern I3); lane M, lambda ladder PFGE marker.
    Figure Legend Snippet: PFGE patterns of recently isolated O3:K6 strains of V. parahaemolyticus . Conditions for PFGE were 1% agarose gel, 0.5× Tris-borate-EDTA buffer, 190 V, pulse time 3 to 80 s, for 22.4 h. Lane 1, isolate 1020 (from Philippines; pattern I5); lane 2, isolate 1021 (from Singapore; pattern I6); lane 3, isolate 1084 (from Taiwan; pattern I7); lane 4, isolate 1104 (from Taiwan; pattern I8); lane 5, isolate 1123 (from Taiwan; pattern I1); lane 6, isolate 1125 (from Taiwan; pattern I1); lane 7, isolate 1139 (from Taiwan; pattern I4); lane 8, isolate 1154 (from Taiwan; pattern I2); lane 9, isolate 97-804 (from Korea; pattern I3); lane M, lambda ladder PFGE marker.

    Techniques Used: Isolation, Agarose Gel Electrophoresis, Marker

    PFGE patterns of O3:K6 strains of V. parahaemolyticus isolated before 1996. (A) Lane 1, isolate AQ3810 (traveler from Singapore; pattern R); lane 2, isolate AQ4019 (traveler from Singapore; pattern A3); lane 3, isolate AQ4037 (traveler from Maldive Islands; pattern A3); lane 4, isolate AQ4093 (traveler from Maldive Islands; pattern A3); lane 5, isolate AQ4133 (traveler from Hong Kong, pattern A3); lane 6, isolate AQ4235 (traveler from Thailand; pattern A1); lane 7, isolate AQ4299 (traveler from Thailand; pattern A1); lane 8, isolate AQ4644 (traveler from Hong Kong; pattern A3); lane M, lambda ladder PFGE marker, 48.5 kb at the bottom with an increment of 48.5 kb. (B) Lane 1, isolate AQ4733 (traveler from Singapore; pattern A2); lane 2, isolate AQ4853 (traveler from Hong Kong; pattern A3); lane M, lambda ladder PFGE marker.
    Figure Legend Snippet: PFGE patterns of O3:K6 strains of V. parahaemolyticus isolated before 1996. (A) Lane 1, isolate AQ3810 (traveler from Singapore; pattern R); lane 2, isolate AQ4019 (traveler from Singapore; pattern A3); lane 3, isolate AQ4037 (traveler from Maldive Islands; pattern A3); lane 4, isolate AQ4093 (traveler from Maldive Islands; pattern A3); lane 5, isolate AQ4133 (traveler from Hong Kong, pattern A3); lane 6, isolate AQ4235 (traveler from Thailand; pattern A1); lane 7, isolate AQ4299 (traveler from Thailand; pattern A1); lane 8, isolate AQ4644 (traveler from Hong Kong; pattern A3); lane M, lambda ladder PFGE marker, 48.5 kb at the bottom with an increment of 48.5 kb. (B) Lane 1, isolate AQ4733 (traveler from Singapore; pattern A2); lane 2, isolate AQ4853 (traveler from Hong Kong; pattern A3); lane M, lambda ladder PFGE marker.

    Techniques Used: Isolation, Marker

    4) Product Images from "Molecular Typing of Beta-Hemolytic Streptococci from Two Patients with Lower-Limb Cellulitis: Identical Isolates from Toe Web and Blood Specimens ▿"

    Article Title: Molecular Typing of Beta-Hemolytic Streptococci from Two Patients with Lower-Limb Cellulitis: Identical Isolates from Toe Web and Blood Specimens ▿

    Journal:

    doi: 10.1128/JCM.00532-07

    PFGE patterns of SmaI digests of streptococcal strains from cellulitis patients. Lane 1, lambda ladder PFG marker; lanes 2 and 3, S. pyogenes from blood and toe web samples, respectively, from patient 1; lanes 4 and 5, S. dysgalactiae subsp. equisimilis
    Figure Legend Snippet: PFGE patterns of SmaI digests of streptococcal strains from cellulitis patients. Lane 1, lambda ladder PFG marker; lanes 2 and 3, S. pyogenes from blood and toe web samples, respectively, from patient 1; lanes 4 and 5, S. dysgalactiae subsp. equisimilis

    Techniques Used: Marker

    5) Product Images from "Adventures in the Enormous: A 1.8 Million Clone BAC Library for the 21.7 Gb Genome of Loblolly Pine"

    Article Title: Adventures in the Enormous: A 1.8 Million Clone BAC Library for the 21.7 Gb Genome of Loblolly Pine

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0016214

    Inserts of LP 7-56 BAC clones. Insert size distribution of clones from (A) plates 1-2650, (B) plates 2651-4752, and (C) the library as a whole. (D) A typical agarose pulsed-field gel showing Not I digests of clones from the latter half of the library. The New England Biolabs PFGE Lambda Ladder is in the lane at the far left. A 7.5 kb vector band is visible at the bottom of each sample lane.
    Figure Legend Snippet: Inserts of LP 7-56 BAC clones. Insert size distribution of clones from (A) plates 1-2650, (B) plates 2651-4752, and (C) the library as a whole. (D) A typical agarose pulsed-field gel showing Not I digests of clones from the latter half of the library. The New England Biolabs PFGE Lambda Ladder is in the lane at the far left. A 7.5 kb vector band is visible at the bottom of each sample lane.

    Techniques Used: BAC Assay, Clone Assay, Pulsed-Field Gel, Plasmid Preparation

    6) Product Images from "Human skin microbiota is a rich source of bacteriocin-producing staphylococci that kill human pathogens"

    Article Title: Human skin microbiota is a rich source of bacteriocin-producing staphylococci that kill human pathogens

    Journal: FEMS Microbiology Ecology

    doi: 10.1093/femsec/fiy241

    PFGE macrodigestion patterns of staphylococcal skin isolates following genomic digestion with Sma 1. Lanes 1–4 = Staphylococcus hominis : APC 2925, 2924, 2939 and 3365; lanes 5–10 = Staphylococcus warneri : APC 2942, 2922, 2937, 2947, 2940 and 2930; lane 11 = Staphylococcus epidermidis : APC 3477; lane 12 = Staphylococcus simulans APC 2926; lanes 13–21 = Staphylococcus capitis APC 2934, 2918, 2923, 2941, 3480, 2946, 2932, 2927 and 3481. Lanes 4, 14 and 21 were taken from other PFGE gels with the same lambda marker (M) and input into this gel. epi = epidermidis; sim = simulans. Artefact in lane 3 below the first band is not a Sma 1 DNA band.
    Figure Legend Snippet: PFGE macrodigestion patterns of staphylococcal skin isolates following genomic digestion with Sma 1. Lanes 1–4 = Staphylococcus hominis : APC 2925, 2924, 2939 and 3365; lanes 5–10 = Staphylococcus warneri : APC 2942, 2922, 2937, 2947, 2940 and 2930; lane 11 = Staphylococcus epidermidis : APC 3477; lane 12 = Staphylococcus simulans APC 2926; lanes 13–21 = Staphylococcus capitis APC 2934, 2918, 2923, 2941, 3480, 2946, 2932, 2927 and 3481. Lanes 4, 14 and 21 were taken from other PFGE gels with the same lambda marker (M) and input into this gel. epi = epidermidis; sim = simulans. Artefact in lane 3 below the first band is not a Sma 1 DNA band.

    Techniques Used: Marker

    7) Product Images from "Dissemination and genetic analysis of the stealthy vanB gene clusters of Enterococcus faecium clinical isolates in Japan"

    Article Title: Dissemination and genetic analysis of the stealthy vanB gene clusters of Enterococcus faecium clinical isolates in Japan

    Journal: BMC Microbiology

    doi: 10.1186/s12866-018-1342-1

    PFGE profiles and dendrogram of the VAN susceptible VanB-type E. faecium isolates. Bacterial DNAs were digested with Sma I and separated by pulsed-field gel electrophoresis (PFGE). The genetic relatedness was analyzed using the Dice coefficient and the dendrogram was constructed with the clustering algorithm of Unweighted Pair-Group Method with an Arithmetic Mean (UPGMA) using FP Quest Software (Bio-Rad). The optimization and the tolerance were 1 and 1.5%, respectively. Major clusters and subclusters of the isolates were delineated with 85 and 90% similarity cutoff values for PFGE as indicated by the vertical solid line and dotted line, respectively. A lambda PFG Ladder (New England BioLabs, MA) was used as the Molecular Marker (MM)
    Figure Legend Snippet: PFGE profiles and dendrogram of the VAN susceptible VanB-type E. faecium isolates. Bacterial DNAs were digested with Sma I and separated by pulsed-field gel electrophoresis (PFGE). The genetic relatedness was analyzed using the Dice coefficient and the dendrogram was constructed with the clustering algorithm of Unweighted Pair-Group Method with an Arithmetic Mean (UPGMA) using FP Quest Software (Bio-Rad). The optimization and the tolerance were 1 and 1.5%, respectively. Major clusters and subclusters of the isolates were delineated with 85 and 90% similarity cutoff values for PFGE as indicated by the vertical solid line and dotted line, respectively. A lambda PFG Ladder (New England BioLabs, MA) was used as the Molecular Marker (MM)

    Techniques Used: Pulsed-Field Gel, Electrophoresis, Construct, Software, Marker

    8) Product Images from "PMQR genes oqxAB and aac(6′)Ib-cr accelerate the development of fluoroquinolone resistance in Salmonella typhimurium"

    Article Title: PMQR genes oqxAB and aac(6′)Ib-cr accelerate the development of fluoroquinolone resistance in Salmonella typhimurium

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2014.00521

    S1-PFGE and southern hybridization of 16SrRNA, oqxA , and aac(6 ′ )Ib-cr on two oqxAB-positive isolates. Arrows indicated chromosomal DNA or plasmids harboring oqxAB and aac(6 ′ )Ib-cr . 06–53 and 10–63 are oqxAB -positive S. typhimurium clinical isolates; M, Lambda PFGE marker.
    Figure Legend Snippet: S1-PFGE and southern hybridization of 16SrRNA, oqxA , and aac(6 ′ )Ib-cr on two oqxAB-positive isolates. Arrows indicated chromosomal DNA or plasmids harboring oqxAB and aac(6 ′ )Ib-cr . 06–53 and 10–63 are oqxAB -positive S. typhimurium clinical isolates; M, Lambda PFGE marker.

    Techniques Used: Hybridization, Marker

    9) Product Images from "First Report of the Local Spread of Vancomycin-Resistant Enterococci Ascribed to the Interspecies Transmission of a vanA Gene Cluster-Carrying Linear Plasmid"

    Article Title: First Report of the Local Spread of Vancomycin-Resistant Enterococci Ascribed to the Interspecies Transmission of a vanA Gene Cluster-Carrying Linear Plasmid

    Journal: mSphere

    doi: 10.1128/mSphere.00102-20

    Pulsed-field gel electrophoresis (PFGE) analysis of S1 nuclease-untreated DNA and Southern blotting of the locally spread strains. PFGE analysis of S1 nuclease-untreated DNA (left) and Southern hybridization with a vanA gene probe (right). Lanes: C, AA708, a control strain harboring the linear plasmid pELF1; MM, Lambda Ladder PFG Marker (New England BioLabs); 1, KUHS1; 2, KUHS2; 3, KUHS3; 4, KUHS4; 5, KUHS5; 6, KUHS6; 7, KUHS7; 8, KUHS8; 9, KUHS9; 10, KUHS10; 11, KUHS11; 12, KUHS12; 13, KUHS13; 14, KUHS14; 15, KUHS15; 16, KUHS16; 17, KUHS17; 18, KUHS18; 19, KUHS19; 20, KUHS20; 21, KUHS21; 22, KUHS22; 23, KUHS23; 24, KUHS24; 25, KUHS25.
    Figure Legend Snippet: Pulsed-field gel electrophoresis (PFGE) analysis of S1 nuclease-untreated DNA and Southern blotting of the locally spread strains. PFGE analysis of S1 nuclease-untreated DNA (left) and Southern hybridization with a vanA gene probe (right). Lanes: C, AA708, a control strain harboring the linear plasmid pELF1; MM, Lambda Ladder PFG Marker (New England BioLabs); 1, KUHS1; 2, KUHS2; 3, KUHS3; 4, KUHS4; 5, KUHS5; 6, KUHS6; 7, KUHS7; 8, KUHS8; 9, KUHS9; 10, KUHS10; 11, KUHS11; 12, KUHS12; 13, KUHS13; 14, KUHS14; 15, KUHS15; 16, KUHS16; 17, KUHS17; 18, KUHS18; 19, KUHS19; 20, KUHS20; 21, KUHS21; 22, KUHS22; 23, KUHS23; 24, KUHS24; 25, KUHS25.

    Techniques Used: Pulsed-Field Gel, Electrophoresis, Southern Blot, Hybridization, Plasmid Preparation, Marker

    10) Product Images from "Molecular Characterization of Gentamicin-Resistant Enterococci in the United States: Evidence of Spread from Animals to Humans through Food"

    Article Title: Molecular Characterization of Gentamicin-Resistant Enterococci in the United States: Evidence of Spread from Animals to Humans through Food

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.41.3.1109-1113.2003

    PFGE of Sma I-digested genomic DNA from gentamicin-resistant E. faecalis isolated from human and ground pork samples in Michigan. Lanes 1 and 9, Lambda ladder DNA standard; lane 2, human isolate; lanes 3 through 8, ground pork isolates with related PFGE patterns.
    Figure Legend Snippet: PFGE of Sma I-digested genomic DNA from gentamicin-resistant E. faecalis isolated from human and ground pork samples in Michigan. Lanes 1 and 9, Lambda ladder DNA standard; lane 2, human isolate; lanes 3 through 8, ground pork isolates with related PFGE patterns.

    Techniques Used: Isolation

    PFGE of Sma I-digested genomic DNA from gentamicin-resistant E. faecalis from grocery store chicken isolates and one human isolate. Lanes 1, 4, and 10, Lambda ladder DNA standard; lanes 2 and 3, human and grocery store chicken isolates, respectively, from Oregon with indistinguishable PFGE patterns; lanes 5 through 7, grocery store chicken isolate from Georgia with indistinguishable PFGE patterns; lanes 8 and 9, grocery store chicken isolates from Maryland and Oregon with indistinguishable PFGE patterns.
    Figure Legend Snippet: PFGE of Sma I-digested genomic DNA from gentamicin-resistant E. faecalis from grocery store chicken isolates and one human isolate. Lanes 1, 4, and 10, Lambda ladder DNA standard; lanes 2 and 3, human and grocery store chicken isolates, respectively, from Oregon with indistinguishable PFGE patterns; lanes 5 through 7, grocery store chicken isolate from Georgia with indistinguishable PFGE patterns; lanes 8 and 9, grocery store chicken isolates from Maryland and Oregon with indistinguishable PFGE patterns.

    Techniques Used:

    11) Product Images from "Molecular Analysis of Isoleucyl-tRNA Synthetase Mutations in Clinical Isolates of Methicillin-Resistant Staphylococcus aureus with Low-Level Mupirocin Resistance"

    Article Title: Molecular Analysis of Isoleucyl-tRNA Synthetase Mutations in Clinical Isolates of Methicillin-Resistant Staphylococcus aureus with Low-Level Mupirocin Resistance

    Journal: Journal of Korean Medical Science

    doi: 10.3346/jkms.2006.21.5.827

    Representative PFGE patterns of Sma I macrorestriction fragments of genomic DNA of MRSA strains with low-level mupirocin resistance isolated from patients in ICUs. Lane 1, λ ladder marker; lane 2 to lane 7 show PFGE patterns A0, A1, B, C, D, and E, respectively.
    Figure Legend Snippet: Representative PFGE patterns of Sma I macrorestriction fragments of genomic DNA of MRSA strains with low-level mupirocin resistance isolated from patients in ICUs. Lane 1, λ ladder marker; lane 2 to lane 7 show PFGE patterns A0, A1, B, C, D, and E, respectively.

    Techniques Used: Isolation, Marker

    Related Articles

    Cell Harvesting:

    Article Title: Molecular Typing of Beta-Hemolytic Streptococci from Two Patients with Lower-Limb Cellulitis: Identical Isolates from Toe Web and Blood Specimens ▿
    Article Snippet: .. Bacterial cell harvesting, preparation, digestion of DNA, and PFGE were performed as described by Sá-Leão , except that PIV buffer (1 M NaCl, 10 mM Tris HCl) was used instead of phosphate buffer for the preparation of cells and agarose plugs, and a lambda ladder PFG marker (New England Biolabs) was used as the molecular weight standard. .. A reference strain of S. dysgalactiae subsp. equisimilis , ATCC 12394, was included in the PFGE for comparison with patient strains of the same species.

    Positive Control:

    Article Title: Biofilm-Forming Clinical Staphylococcus Isolates Harbor Horizontal Transfer and Antibiotic Resistance Genes
    Article Snippet: .. The reaction was stopped by transferring the slices to 1 ml of TE buffer for 1 h. Digested slices were applied to wells in 1% (w/v) Pulsed Field Certified Agarose (BioRad) prepared in 0.5 × TBE buffer [45 mM Tris (pH 8), 45 mM boric acid, 1 mM EDTA] and run in CHEF-DR® III System (BioRad) at 6 V/cm, a field angle of 120°, and switch times of 5 to 35 s for 22 h. Lambda Ladder PFGE (New England Biolabs, Ispwich, U.S) was used as molecular size marker and pSK41 plasmid (46.4 kb) was used as positive control. .. Gels were stained with GelRed Nucleic Acid Stain (Biogen Científica, Madrid, Spain).

    Transferring:

    Article Title: Biofilm-Forming Clinical Staphylococcus Isolates Harbor Horizontal Transfer and Antibiotic Resistance Genes
    Article Snippet: .. The reaction was stopped by transferring the slices to 1 ml of TE buffer for 1 h. Digested slices were applied to wells in 1% (w/v) Pulsed Field Certified Agarose (BioRad) prepared in 0.5 × TBE buffer [45 mM Tris (pH 8), 45 mM boric acid, 1 mM EDTA] and run in CHEF-DR® III System (BioRad) at 6 V/cm, a field angle of 120°, and switch times of 5 to 35 s for 22 h. Lambda Ladder PFGE (New England Biolabs, Ispwich, U.S) was used as molecular size marker and pSK41 plasmid (46.4 kb) was used as positive control. .. Gels were stained with GelRed Nucleic Acid Stain (Biogen Científica, Madrid, Spain).

    Electrophoresis:

    Article Title: PMQR genes oqxAB and aac(6′)Ib-cr accelerate the development of fluoroquinolone resistance in Salmonella typhimurium
    Article Snippet: .. Briefly, agarose-embedded DNA was digested with S1 nuclease (New England BioLab) at 37°C for 1 h. The restriction fragments were separated by electrophoresis in 0.5 Tris-borate-EDTA buffer at 14°C for 18 h using a Chef Mapper electrophoresis system (Bio-Rad, Hercules, CA, USA) with pulse times of 2.16 to 63.8 s. Phage Lambda PFGE ladder (New England BioLab) was used as DNA size marker. .. The gels were stained with GelRed, and DNA bands were visualized with UV transillumination (Bio-Rad).

    Plasmid Preparation:

    Article Title: Biofilm-Forming Clinical Staphylococcus Isolates Harbor Horizontal Transfer and Antibiotic Resistance Genes
    Article Snippet: .. The reaction was stopped by transferring the slices to 1 ml of TE buffer for 1 h. Digested slices were applied to wells in 1% (w/v) Pulsed Field Certified Agarose (BioRad) prepared in 0.5 × TBE buffer [45 mM Tris (pH 8), 45 mM boric acid, 1 mM EDTA] and run in CHEF-DR® III System (BioRad) at 6 V/cm, a field angle of 120°, and switch times of 5 to 35 s for 22 h. Lambda Ladder PFGE (New England Biolabs, Ispwich, U.S) was used as molecular size marker and pSK41 plasmid (46.4 kb) was used as positive control. .. Gels were stained with GelRed Nucleic Acid Stain (Biogen Científica, Madrid, Spain).

    Marker:

    Article Title: Dissemination and genetic analysis of the stealthy vanB gene clusters of Enterococcus faecium clinical isolates in Japan
    Article Snippet: .. A lambda PFG Ladder (New England BioLabs, MA) was used as the Molecular Marker (MM). .. Southern transfer and hybridization analysis PFGE analyses with S1 nuclease or I-Ceu I were performed as described above.

    Article Title: Biofilm-Forming Clinical Staphylococcus Isolates Harbor Horizontal Transfer and Antibiotic Resistance Genes
    Article Snippet: .. The reaction was stopped by transferring the slices to 1 ml of TE buffer for 1 h. Digested slices were applied to wells in 1% (w/v) Pulsed Field Certified Agarose (BioRad) prepared in 0.5 × TBE buffer [45 mM Tris (pH 8), 45 mM boric acid, 1 mM EDTA] and run in CHEF-DR® III System (BioRad) at 6 V/cm, a field angle of 120°, and switch times of 5 to 35 s for 22 h. Lambda Ladder PFGE (New England Biolabs, Ispwich, U.S) was used as molecular size marker and pSK41 plasmid (46.4 kb) was used as positive control. .. Gels were stained with GelRed Nucleic Acid Stain (Biogen Científica, Madrid, Spain).

    Article Title: Human skin microbiota is a rich source of bacteriocin-producing staphylococci that kill human pathogens
    Article Snippet: .. Lambda PFGE marker (New England Biolabs) was loaded onto the gel as described above and used as a reference ladder. ..

    Article Title: PMQR genes oqxAB and aac(6′)Ib-cr accelerate the development of fluoroquinolone resistance in Salmonella typhimurium
    Article Snippet: .. Briefly, agarose-embedded DNA was digested with S1 nuclease (New England BioLab) at 37°C for 1 h. The restriction fragments were separated by electrophoresis in 0.5 Tris-borate-EDTA buffer at 14°C for 18 h using a Chef Mapper electrophoresis system (Bio-Rad, Hercules, CA, USA) with pulse times of 2.16 to 63.8 s. Phage Lambda PFGE ladder (New England BioLab) was used as DNA size marker. .. The gels were stained with GelRed, and DNA bands were visualized with UV transillumination (Bio-Rad).

    Article Title: Characteristics of Vibrio parahaemolyticus O3:K6 from Asia
    Article Snippet: .. A lambda ladder PFGE marker (New England BioLabs) was used to mark molecular size. .. After electrophoresis was performed, gels were stained in ethidium bromide (Sigma Co., St. Louis, Mo.), destained in distilled water, and photographed with a UV transilluminator, the Flou-Link 312 (Vilber Lourmat, Torey, France).

    Article Title: Molecular Typing of Beta-Hemolytic Streptococci from Two Patients with Lower-Limb Cellulitis: Identical Isolates from Toe Web and Blood Specimens ▿
    Article Snippet: .. Bacterial cell harvesting, preparation, digestion of DNA, and PFGE were performed as described by Sá-Leão , except that PIV buffer (1 M NaCl, 10 mM Tris HCl) was used instead of phosphate buffer for the preparation of cells and agarose plugs, and a lambda ladder PFG marker (New England Biolabs) was used as the molecular weight standard. .. A reference strain of S. dysgalactiae subsp. equisimilis , ATCC 12394, was included in the PFGE for comparison with patient strains of the same species.

    Molecular Weight:

    Article Title: Molecular Typing of Beta-Hemolytic Streptococci from Two Patients with Lower-Limb Cellulitis: Identical Isolates from Toe Web and Blood Specimens ▿
    Article Snippet: .. Bacterial cell harvesting, preparation, digestion of DNA, and PFGE were performed as described by Sá-Leão , except that PIV buffer (1 M NaCl, 10 mM Tris HCl) was used instead of phosphate buffer for the preparation of cells and agarose plugs, and a lambda ladder PFG marker (New England Biolabs) was used as the molecular weight standard. .. A reference strain of S. dysgalactiae subsp. equisimilis , ATCC 12394, was included in the PFGE for comparison with patient strains of the same species.

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    New England Biolabs lambda ladder pfge
    Detection of plasmids in 25 staphylococcal clinical isolates by <t>PFGE.</t> Large plasmids ( > 30 kb) were analyzed by PFGE after digestion with nuclease S1 at 37°C for 45 min. Lanes 1–25: clinical isolates; lane number corresponds to the number of the isolate. Lane C: positive control, plasmid pSK41 (46.4 kb) extracted from SK5428 strain. Lane M: <t>Lambda</t> Ladder PFGE molecular size marker. Arrows point to detected plasmids.
    Lambda Ladder Pfge, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lambda ladder pfge/product/New England Biolabs
    Average 96 stars, based on 49 article reviews
    Price from $9.99 to $1999.99
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    Detection of plasmids in 25 staphylococcal clinical isolates by PFGE. Large plasmids ( > 30 kb) were analyzed by PFGE after digestion with nuclease S1 at 37°C for 45 min. Lanes 1–25: clinical isolates; lane number corresponds to the number of the isolate. Lane C: positive control, plasmid pSK41 (46.4 kb) extracted from SK5428 strain. Lane M: Lambda Ladder PFGE molecular size marker. Arrows point to detected plasmids.

    Journal: Frontiers in Microbiology

    Article Title: Biofilm-Forming Clinical Staphylococcus Isolates Harbor Horizontal Transfer and Antibiotic Resistance Genes

    doi: 10.3389/fmicb.2017.02018

    Figure Lengend Snippet: Detection of plasmids in 25 staphylococcal clinical isolates by PFGE. Large plasmids ( > 30 kb) were analyzed by PFGE after digestion with nuclease S1 at 37°C for 45 min. Lanes 1–25: clinical isolates; lane number corresponds to the number of the isolate. Lane C: positive control, plasmid pSK41 (46.4 kb) extracted from SK5428 strain. Lane M: Lambda Ladder PFGE molecular size marker. Arrows point to detected plasmids.

    Article Snippet: The reaction was stopped by transferring the slices to 1 ml of TE buffer for 1 h. Digested slices were applied to wells in 1% (w/v) Pulsed Field Certified Agarose (BioRad) prepared in 0.5 × TBE buffer [45 mM Tris (pH 8), 45 mM boric acid, 1 mM EDTA] and run in CHEF-DR® III System (BioRad) at 6 V/cm, a field angle of 120°, and switch times of 5 to 35 s for 22 h. Lambda Ladder PFGE (New England Biolabs, Ispwich, U.S) was used as molecular size marker and pSK41 plasmid (46.4 kb) was used as positive control.

    Techniques: Positive Control, Plasmid Preparation, Marker

    C-1027 BGCs residing on giant plasmids of varying size in the five C-1027 producers. (A) PFGE analysis of the five C-1027 producers showing the existence of giant plasmids of varying sizes. (B) Southern analysis revealing that the C-1027 BGCs all reside on giant plasmids in the five producers. M, Lambda PFG ladder (NEB); lane 1, S. globisporus wild-type; lane 2, S. globisporus AF40; lane 3, CB00657; lane 4, CB02329; lane 5, CB02366; and lane 6, CB03608.

    Journal: Journal of natural products

    Article Title: Discovery of Alternative Producers of the Enediyne Antitumor Antibiotic C-1027 with High Titers

    doi: 10.1021/acs.jnatprod.7b01013

    Figure Lengend Snippet: C-1027 BGCs residing on giant plasmids of varying size in the five C-1027 producers. (A) PFGE analysis of the five C-1027 producers showing the existence of giant plasmids of varying sizes. (B) Southern analysis revealing that the C-1027 BGCs all reside on giant plasmids in the five producers. M, Lambda PFG ladder (NEB); lane 1, S. globisporus wild-type; lane 2, S. globisporus AF40; lane 3, CB00657; lane 4, CB02329; lane 5, CB02366; and lane 6, CB03608.

    Article Snippet: Lambda PFG Ladder (New England Biolabs) was used as the size standard.

    Techniques:

    PFGE patterns of recently isolated O3:K6 strains of V. parahaemolyticus . Conditions for PFGE were 1% agarose gel, 0.5× Tris-borate-EDTA buffer, 190 V, pulse time 3 to 80 s, for 22.4 h. Lane 1, isolate 1020 (from Philippines; pattern I5); lane 2, isolate 1021 (from Singapore; pattern I6); lane 3, isolate 1084 (from Taiwan; pattern I7); lane 4, isolate 1104 (from Taiwan; pattern I8); lane 5, isolate 1123 (from Taiwan; pattern I1); lane 6, isolate 1125 (from Taiwan; pattern I1); lane 7, isolate 1139 (from Taiwan; pattern I4); lane 8, isolate 1154 (from Taiwan; pattern I2); lane 9, isolate 97-804 (from Korea; pattern I3); lane M, lambda ladder PFGE marker.

    Journal: Applied and Environmental Microbiology

    Article Title: Characteristics of Vibrio parahaemolyticus O3:K6 from Asia

    doi:

    Figure Lengend Snippet: PFGE patterns of recently isolated O3:K6 strains of V. parahaemolyticus . Conditions for PFGE were 1% agarose gel, 0.5× Tris-borate-EDTA buffer, 190 V, pulse time 3 to 80 s, for 22.4 h. Lane 1, isolate 1020 (from Philippines; pattern I5); lane 2, isolate 1021 (from Singapore; pattern I6); lane 3, isolate 1084 (from Taiwan; pattern I7); lane 4, isolate 1104 (from Taiwan; pattern I8); lane 5, isolate 1123 (from Taiwan; pattern I1); lane 6, isolate 1125 (from Taiwan; pattern I1); lane 7, isolate 1139 (from Taiwan; pattern I4); lane 8, isolate 1154 (from Taiwan; pattern I2); lane 9, isolate 97-804 (from Korea; pattern I3); lane M, lambda ladder PFGE marker.

    Article Snippet: A lambda ladder PFGE marker (New England BioLabs) was used to mark molecular size.

    Techniques: Isolation, Agarose Gel Electrophoresis, Marker

    PFGE patterns of O3:K6 strains of V. parahaemolyticus isolated before 1996. (A) Lane 1, isolate AQ3810 (traveler from Singapore; pattern R); lane 2, isolate AQ4019 (traveler from Singapore; pattern A3); lane 3, isolate AQ4037 (traveler from Maldive Islands; pattern A3); lane 4, isolate AQ4093 (traveler from Maldive Islands; pattern A3); lane 5, isolate AQ4133 (traveler from Hong Kong, pattern A3); lane 6, isolate AQ4235 (traveler from Thailand; pattern A1); lane 7, isolate AQ4299 (traveler from Thailand; pattern A1); lane 8, isolate AQ4644 (traveler from Hong Kong; pattern A3); lane M, lambda ladder PFGE marker, 48.5 kb at the bottom with an increment of 48.5 kb. (B) Lane 1, isolate AQ4733 (traveler from Singapore; pattern A2); lane 2, isolate AQ4853 (traveler from Hong Kong; pattern A3); lane M, lambda ladder PFGE marker.

    Journal: Applied and Environmental Microbiology

    Article Title: Characteristics of Vibrio parahaemolyticus O3:K6 from Asia

    doi:

    Figure Lengend Snippet: PFGE patterns of O3:K6 strains of V. parahaemolyticus isolated before 1996. (A) Lane 1, isolate AQ3810 (traveler from Singapore; pattern R); lane 2, isolate AQ4019 (traveler from Singapore; pattern A3); lane 3, isolate AQ4037 (traveler from Maldive Islands; pattern A3); lane 4, isolate AQ4093 (traveler from Maldive Islands; pattern A3); lane 5, isolate AQ4133 (traveler from Hong Kong, pattern A3); lane 6, isolate AQ4235 (traveler from Thailand; pattern A1); lane 7, isolate AQ4299 (traveler from Thailand; pattern A1); lane 8, isolate AQ4644 (traveler from Hong Kong; pattern A3); lane M, lambda ladder PFGE marker, 48.5 kb at the bottom with an increment of 48.5 kb. (B) Lane 1, isolate AQ4733 (traveler from Singapore; pattern A2); lane 2, isolate AQ4853 (traveler from Hong Kong; pattern A3); lane M, lambda ladder PFGE marker.

    Article Snippet: A lambda ladder PFGE marker (New England BioLabs) was used to mark molecular size.

    Techniques: Isolation, Marker

    PFGE patterns of SmaI digests of streptococcal strains from cellulitis patients. Lane 1, lambda ladder PFG marker; lanes 2 and 3, S. pyogenes from blood and toe web samples, respectively, from patient 1; lanes 4 and 5, S. dysgalactiae subsp. equisimilis

    Journal:

    Article Title: Molecular Typing of Beta-Hemolytic Streptococci from Two Patients with Lower-Limb Cellulitis: Identical Isolates from Toe Web and Blood Specimens ▿

    doi: 10.1128/JCM.00532-07

    Figure Lengend Snippet: PFGE patterns of SmaI digests of streptococcal strains from cellulitis patients. Lane 1, lambda ladder PFG marker; lanes 2 and 3, S. pyogenes from blood and toe web samples, respectively, from patient 1; lanes 4 and 5, S. dysgalactiae subsp. equisimilis

    Article Snippet: Bacterial cell harvesting, preparation, digestion of DNA, and PFGE were performed as described by Sá-Leão , except that PIV buffer (1 M NaCl, 10 mM Tris HCl) was used instead of phosphate buffer for the preparation of cells and agarose plugs, and a lambda ladder PFG marker (New England Biolabs) was used as the molecular weight standard.

    Techniques: Marker