psv40 gluc control plasmid  (New England Biolabs)


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    Name:
    pSV40 GLuc Control Plasmid
    Description:
    pSV40 GLuc Control Plasmid 20 ug
    Catalog Number:
    n0323s
    Price:
    274
    Size:
    20 ug
    Category:
    Cellular Biology
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    Structured Review

    New England Biolabs psv40 gluc control plasmid
    pSV40 GLuc Control Plasmid
    pSV40 GLuc Control Plasmid 20 ug
    https://www.bioz.com/result/psv40 gluc control plasmid/product/New England Biolabs
    Average 94 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    psv40 gluc control plasmid - by Bioz Stars, 2020-08
    94/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Exogenous marker-engineered mesenchymal stem cells detect cancer and metastases in a simple blood assay
    Article Snippet: .. The sequences of interest from pUCBB-eGFP (#32548; Addgene, Cambridge, MA, USA), pcDNA3.1(+)/Luc2=tdT (#32904; Addgene), and pSV40-Gluc (New England BioLabs, Ipswich, MA, USA) were cloned into the promoterless LV transfer vector LV-PL4 (GenTarget). .. Lentiviral transduction All LV constructs were packaged (pMD2.G, #12259; pRSV-Rev, #12253; pMDLg/pRRE, #12251; all from Addgene) as LV vectors in 293 T-LV cells [ ] by using Lipofectamine LTX and PLUS™ Reagents (Life Technologies).

    Article Title: Cell-Based High-Throughput Screening Assay Identifies 2′,2′-Difluoro-2′-deoxycytidine Gemcitabine as a Potential Antipoliovirus Agent
    Article Snippet: .. Gaussia Luciferase ( GLuc ) gene was cloned from pSV40-GLuc control plasmid (New England BioLabs). .. GLuc was inserted into the end of the VP1 region by overlap extension PCR method using the following primers: 5′-GGATGGTACGCTTACACCCCTCTCCACCAAGGACTTAACGACTTACGGTTTTGGTCATGGCGGAGGTGG-GGGAGGTGAATTCATGGGAGTCAAAGTTCTGTTTGCCC-3′ and 5′-CCGCTTTGTTTTGGTGTCCGAATCCATATGTGGTCAGATCCTCGAGGTCACCACCGGCCCCCTT-3′.

    Transfection:

    Article Title: ETV7 represses a subset of interferon-stimulated genes that restrict influenza viruses
    Article Snippet: .. RNA sequencing 293T cells were transfected with ETV7- or control-expressing plasmids and selected using puromycin (20 μg/mL) for 24 h before treatment with IFN-α (100 U/mL). .. Total RNA was collected at 9 h post-IFN treatment using Monarch Total RNA Miniprep Kits (NEB).

    Article Title: Chemically Modified Variants of Fenofibrate with Antiglioblastoma Potential
    Article Snippet: .. Together with JsTkpGL3 plasmid HepG2 cells were transfected with pSV40-GLuc (New England Biolabs., Ipswich, MA) control plasmid expressing Gaussia luciferase under the control of the constitutive SV40 early promoter, used to normalize for efficiency of transfection. .. Twenty-four hours after transfection, the cells were incubated with ciglitazone (30 μM), FF, PP1, PP2, PP3, and PP4 (all 25 μM) for an additional 24 hours.

    Article Title: Function of inhibitor of Bruton's tyrosine kinase isoform α (IBTKα) in nonalcoholic steatohepatitis links autophagy and the unfolded protein response
    Article Snippet: .. For the Gaussia luciferase assay, PSV40-gluc control plasmid from New England Biolabs (Ipswich, MA) was transfected to cells, and supernatant was used to measure secreted luciferase. .. Human hepatoma HepG2 cells were purchased from ATCC and were both cultured and treated with 600 μ m FFAs as described previously ( ).

    Luciferase:

    Article Title: Chemically Modified Variants of Fenofibrate with Antiglioblastoma Potential
    Article Snippet: .. Together with JsTkpGL3 plasmid HepG2 cells were transfected with pSV40-GLuc (New England Biolabs., Ipswich, MA) control plasmid expressing Gaussia luciferase under the control of the constitutive SV40 early promoter, used to normalize for efficiency of transfection. .. Twenty-four hours after transfection, the cells were incubated with ciglitazone (30 μM), FF, PP1, PP2, PP3, and PP4 (all 25 μM) for an additional 24 hours.

    Article Title: Function of inhibitor of Bruton's tyrosine kinase isoform α (IBTKα) in nonalcoholic steatohepatitis links autophagy and the unfolded protein response
    Article Snippet: .. For the Gaussia luciferase assay, PSV40-gluc control plasmid from New England Biolabs (Ipswich, MA) was transfected to cells, and supernatant was used to measure secreted luciferase. .. Human hepatoma HepG2 cells were purchased from ATCC and were both cultured and treated with 600 μ m FFAs as described previously ( ).

    Article Title: Cell-Based High-Throughput Screening Assay Identifies 2′,2′-Difluoro-2′-deoxycytidine Gemcitabine as a Potential Antipoliovirus Agent
    Article Snippet: .. Gaussia Luciferase ( GLuc ) gene was cloned from pSV40-GLuc control plasmid (New England BioLabs). .. GLuc was inserted into the end of the VP1 region by overlap extension PCR method using the following primers: 5′-GGATGGTACGCTTACACCCCTCTCCACCAAGGACTTAACGACTTACGGTTTTGGTCATGGCGGAGGTGG-GGGAGGTGAATTCATGGGAGTCAAAGTTCTGTTTGCCC-3′ and 5′-CCGCTTTGTTTTGGTGTCCGAATCCATATGTGGTCAGATCCTCGAGGTCACCACCGGCCCCCTT-3′.

    Article Title: Sertraline induces endoplasmic reticulum stress in hepatic cells
    Article Snippet: .. CMV-SEAP plasmid carrying secreted embryonic alkaline phosphatase (SEAP) (as template to amplify SEAP) was purchased from Addgene (Cambridge, MA) and pSV40-Gluc plasmid carrying secreted Gaussia luciferase (Gluc) (as template to amplify Gluc) was purchased from New England Biolabs. .. The cDNAs of SEAP and Gluc were amplified by PCR and subcloned into BamHI and NheI restriction sites of pLv-EF1α-MCS-IRES-Puro expression vector following the manufacturer’s instructions.

    RNA Sequencing Assay:

    Article Title: ETV7 represses a subset of interferon-stimulated genes that restrict influenza viruses
    Article Snippet: .. RNA sequencing 293T cells were transfected with ETV7- or control-expressing plasmids and selected using puromycin (20 μg/mL) for 24 h before treatment with IFN-α (100 U/mL). .. Total RNA was collected at 9 h post-IFN treatment using Monarch Total RNA Miniprep Kits (NEB).

    Expressing:

    Article Title: Chemically Modified Variants of Fenofibrate with Antiglioblastoma Potential
    Article Snippet: .. Together with JsTkpGL3 plasmid HepG2 cells were transfected with pSV40-GLuc (New England Biolabs., Ipswich, MA) control plasmid expressing Gaussia luciferase under the control of the constitutive SV40 early promoter, used to normalize for efficiency of transfection. .. Twenty-four hours after transfection, the cells were incubated with ciglitazone (30 μM), FF, PP1, PP2, PP3, and PP4 (all 25 μM) for an additional 24 hours.

    Plasmid Preparation:

    Article Title: Chemically Modified Variants of Fenofibrate with Antiglioblastoma Potential
    Article Snippet: .. Together with JsTkpGL3 plasmid HepG2 cells were transfected with pSV40-GLuc (New England Biolabs., Ipswich, MA) control plasmid expressing Gaussia luciferase under the control of the constitutive SV40 early promoter, used to normalize for efficiency of transfection. .. Twenty-four hours after transfection, the cells were incubated with ciglitazone (30 μM), FF, PP1, PP2, PP3, and PP4 (all 25 μM) for an additional 24 hours.

    Article Title: Function of inhibitor of Bruton's tyrosine kinase isoform α (IBTKα) in nonalcoholic steatohepatitis links autophagy and the unfolded protein response
    Article Snippet: .. For the Gaussia luciferase assay, PSV40-gluc control plasmid from New England Biolabs (Ipswich, MA) was transfected to cells, and supernatant was used to measure secreted luciferase. .. Human hepatoma HepG2 cells were purchased from ATCC and were both cultured and treated with 600 μ m FFAs as described previously ( ).

    Article Title: Exogenous marker-engineered mesenchymal stem cells detect cancer and metastases in a simple blood assay
    Article Snippet: .. The sequences of interest from pUCBB-eGFP (#32548; Addgene, Cambridge, MA, USA), pcDNA3.1(+)/Luc2=tdT (#32904; Addgene), and pSV40-Gluc (New England BioLabs, Ipswich, MA, USA) were cloned into the promoterless LV transfer vector LV-PL4 (GenTarget). .. Lentiviral transduction All LV constructs were packaged (pMD2.G, #12259; pRSV-Rev, #12253; pMDLg/pRRE, #12251; all from Addgene) as LV vectors in 293 T-LV cells [ ] by using Lipofectamine LTX and PLUS™ Reagents (Life Technologies).

    Article Title: Cell-Based High-Throughput Screening Assay Identifies 2′,2′-Difluoro-2′-deoxycytidine Gemcitabine as a Potential Antipoliovirus Agent
    Article Snippet: .. Gaussia Luciferase ( GLuc ) gene was cloned from pSV40-GLuc control plasmid (New England BioLabs). .. GLuc was inserted into the end of the VP1 region by overlap extension PCR method using the following primers: 5′-GGATGGTACGCTTACACCCCTCTCCACCAAGGACTTAACGACTTACGGTTTTGGTCATGGCGGAGGTGG-GGGAGGTGAATTCATGGGAGTCAAAGTTCTGTTTGCCC-3′ and 5′-CCGCTTTGTTTTGGTGTCCGAATCCATATGTGGTCAGATCCTCGAGGTCACCACCGGCCCCCTT-3′.

    Article Title: Sertraline induces endoplasmic reticulum stress in hepatic cells
    Article Snippet: .. CMV-SEAP plasmid carrying secreted embryonic alkaline phosphatase (SEAP) (as template to amplify SEAP) was purchased from Addgene (Cambridge, MA) and pSV40-Gluc plasmid carrying secreted Gaussia luciferase (Gluc) (as template to amplify Gluc) was purchased from New England Biolabs. .. The cDNAs of SEAP and Gluc were amplified by PCR and subcloned into BamHI and NheI restriction sites of pLv-EF1α-MCS-IRES-Puro expression vector following the manufacturer’s instructions.

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  • About
  • News
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  • Team
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  • Contact
  • Bioz Stars
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  • 94
    New England Biolabs psv40 gluc control plasmid
    Psv40 Gluc Control Plasmid, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/psv40 gluc control plasmid/product/New England Biolabs
    Average 94 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    psv40 gluc control plasmid - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

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