plasmid expressing gluc  (New England Biolabs)


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    Structured Review

    New England Biolabs plasmid expressing gluc
    Inhibition of cellular host gene expression. MDCK cells (96-well plates; 5.0 × 10 4 cells/well; quadruplicates) were transiently <t>transfected</t> with 50 ng of <t>pCAGGS-Gluc</t> plasmid. After 6 h, transfection medium was replaced with medium containing serial 3-fold dilutions (starting concentration 50 μM) of the indicated compounds. At 24 h posttransfection (hpt), Gluc expression levels were determined from tissue culture supernatants. Cells treated with 0.1% DMSO vehicle were used as an internal control. The EC 50 was calculated as a percentage relative to values obtained with DMSO vehicle-treated cells. Dotted lines indicate 50% inhibition of reporter gene expression (Gluc). Data are expressed as mean and SD from three independent experiments conducted in quadruplicates.
    Plasmid Expressing Gluc, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid expressing gluc/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    plasmid expressing gluc - by Bioz Stars, 2022-07
    86/100 stars

    Images

    1) Product Images from "Identification and Characterization of Novel Compounds with Broad-Spectrum Antiviral Activity against Influenza A and B Viruses"

    Article Title: Identification and Characterization of Novel Compounds with Broad-Spectrum Antiviral Activity against Influenza A and B Viruses

    Journal: Journal of Virology

    doi: 10.1128/JVI.02149-19

    Inhibition of cellular host gene expression. MDCK cells (96-well plates; 5.0 × 10 4 cells/well; quadruplicates) were transiently transfected with 50 ng of pCAGGS-Gluc plasmid. After 6 h, transfection medium was replaced with medium containing serial 3-fold dilutions (starting concentration 50 μM) of the indicated compounds. At 24 h posttransfection (hpt), Gluc expression levels were determined from tissue culture supernatants. Cells treated with 0.1% DMSO vehicle were used as an internal control. The EC 50 was calculated as a percentage relative to values obtained with DMSO vehicle-treated cells. Dotted lines indicate 50% inhibition of reporter gene expression (Gluc). Data are expressed as mean and SD from three independent experiments conducted in quadruplicates.
    Figure Legend Snippet: Inhibition of cellular host gene expression. MDCK cells (96-well plates; 5.0 × 10 4 cells/well; quadruplicates) were transiently transfected with 50 ng of pCAGGS-Gluc plasmid. After 6 h, transfection medium was replaced with medium containing serial 3-fold dilutions (starting concentration 50 μM) of the indicated compounds. At 24 h posttransfection (hpt), Gluc expression levels were determined from tissue culture supernatants. Cells treated with 0.1% DMSO vehicle were used as an internal control. The EC 50 was calculated as a percentage relative to values obtained with DMSO vehicle-treated cells. Dotted lines indicate 50% inhibition of reporter gene expression (Gluc). Data are expressed as mean and SD from three independent experiments conducted in quadruplicates.

    Techniques Used: Inhibition, Expressing, Transfection, Plasmid Preparation, Concentration Assay

    Inhibition of viral replication and transcription. Human 293T cells (96-well plate format; 5.0 × 10 4 cells/well; quadruplicates) were transiently transfected with 125 ng of ambisense pDZ plasmids encoding PR8 PB2, PB1, PA, and NP, together with 250 ng of hpPol-I Gluc and hpPol-I GFP vRNA-like expression plasmids and 50 ng of pCAGGS-Cluc. Cells transfected in the absence of pDZ-PB2 were used as a negative control. After 6 h, transfection medium was replaced with medium containing 3-fold serial dilutions of the indicated compounds (starting concentration of 50 μM). At 24 h posttransfection, Gluc and Cluc expression levels were determined from tissue culture supernatants from transfected cells. Transfected cells were also imaged for GFP expression using a fluorescence microscope. Cells treated with 0.1% DMSO vehicle were used as an internal control. The EC 50 was calculated as a percentage relative to values obtained with DMSO vehicle-treated cells. Dotted lines indicate 50% inhibition viral replication and transcription. Data are expressed as mean and SD from three independent experiments conducted in quadruplicate. Bar, 50 μm.
    Figure Legend Snippet: Inhibition of viral replication and transcription. Human 293T cells (96-well plate format; 5.0 × 10 4 cells/well; quadruplicates) were transiently transfected with 125 ng of ambisense pDZ plasmids encoding PR8 PB2, PB1, PA, and NP, together with 250 ng of hpPol-I Gluc and hpPol-I GFP vRNA-like expression plasmids and 50 ng of pCAGGS-Cluc. Cells transfected in the absence of pDZ-PB2 were used as a negative control. After 6 h, transfection medium was replaced with medium containing 3-fold serial dilutions of the indicated compounds (starting concentration of 50 μM). At 24 h posttransfection, Gluc and Cluc expression levels were determined from tissue culture supernatants from transfected cells. Transfected cells were also imaged for GFP expression using a fluorescence microscope. Cells treated with 0.1% DMSO vehicle were used as an internal control. The EC 50 was calculated as a percentage relative to values obtained with DMSO vehicle-treated cells. Dotted lines indicate 50% inhibition viral replication and transcription. Data are expressed as mean and SD from three independent experiments conducted in quadruplicate. Bar, 50 μm.

    Techniques Used: Inhibition, Transfection, Expressing, Negative Control, Concentration Assay, Fluorescence, Microscopy

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    New England Biolabs plasmid expressing gluc
    Inhibition of cellular host gene expression. MDCK cells (96-well plates; 5.0 × 10 4 cells/well; quadruplicates) were transiently <t>transfected</t> with 50 ng of <t>pCAGGS-Gluc</t> plasmid. After 6 h, transfection medium was replaced with medium containing serial 3-fold dilutions (starting concentration 50 μM) of the indicated compounds. At 24 h posttransfection (hpt), Gluc expression levels were determined from tissue culture supernatants. Cells treated with 0.1% DMSO vehicle were used as an internal control. The EC 50 was calculated as a percentage relative to values obtained with DMSO vehicle-treated cells. Dotted lines indicate 50% inhibition of reporter gene expression (Gluc). Data are expressed as mean and SD from three independent experiments conducted in quadruplicates.
    Plasmid Expressing Gluc, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid expressing gluc/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    plasmid expressing gluc - by Bioz Stars, 2022-07
    86/100 stars
      Buy from Supplier

    86
    New England Biolabs pgluc basic plasmid
    Inhibition of cellular host gene expression. MDCK cells (96-well plates; 5.0 × 10 4 cells/well; quadruplicates) were transiently <t>transfected</t> with 50 ng of <t>pCAGGS-Gluc</t> plasmid. After 6 h, transfection medium was replaced with medium containing serial 3-fold dilutions (starting concentration 50 μM) of the indicated compounds. At 24 h posttransfection (hpt), Gluc expression levels were determined from tissue culture supernatants. Cells treated with 0.1% DMSO vehicle were used as an internal control. The EC 50 was calculated as a percentage relative to values obtained with DMSO vehicle-treated cells. Dotted lines indicate 50% inhibition of reporter gene expression (Gluc). Data are expressed as mean and SD from three independent experiments conducted in quadruplicates.
    Pgluc Basic Plasmid, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgluc basic plasmid/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pgluc basic plasmid - by Bioz Stars, 2022-07
    86/100 stars
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    86
    New England Biolabs psv40 gluc control plasmid
    Inhibition of cellular host gene expression. MDCK cells (96-well plates; 5.0 × 10 4 cells/well; quadruplicates) were transiently <t>transfected</t> with 50 ng of <t>pCAGGS-Gluc</t> plasmid. After 6 h, transfection medium was replaced with medium containing serial 3-fold dilutions (starting concentration 50 μM) of the indicated compounds. At 24 h posttransfection (hpt), Gluc expression levels were determined from tissue culture supernatants. Cells treated with 0.1% DMSO vehicle were used as an internal control. The EC 50 was calculated as a percentage relative to values obtained with DMSO vehicle-treated cells. Dotted lines indicate 50% inhibition of reporter gene expression (Gluc). Data are expressed as mean and SD from three independent experiments conducted in quadruplicates.
    Psv40 Gluc Control Plasmid, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/psv40 gluc control plasmid/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    psv40 gluc control plasmid - by Bioz Stars, 2022-07
    86/100 stars
      Buy from Supplier

    Image Search Results


    Inhibition of cellular host gene expression. MDCK cells (96-well plates; 5.0 × 10 4 cells/well; quadruplicates) were transiently transfected with 50 ng of pCAGGS-Gluc plasmid. After 6 h, transfection medium was replaced with medium containing serial 3-fold dilutions (starting concentration 50 μM) of the indicated compounds. At 24 h posttransfection (hpt), Gluc expression levels were determined from tissue culture supernatants. Cells treated with 0.1% DMSO vehicle were used as an internal control. The EC 50 was calculated as a percentage relative to values obtained with DMSO vehicle-treated cells. Dotted lines indicate 50% inhibition of reporter gene expression (Gluc). Data are expressed as mean and SD from three independent experiments conducted in quadruplicates.

    Journal: Journal of Virology

    Article Title: Identification and Characterization of Novel Compounds with Broad-Spectrum Antiviral Activity against Influenza A and B Viruses

    doi: 10.1128/JVI.02149-19

    Figure Lengend Snippet: Inhibition of cellular host gene expression. MDCK cells (96-well plates; 5.0 × 10 4 cells/well; quadruplicates) were transiently transfected with 50 ng of pCAGGS-Gluc plasmid. After 6 h, transfection medium was replaced with medium containing serial 3-fold dilutions (starting concentration 50 μM) of the indicated compounds. At 24 h posttransfection (hpt), Gluc expression levels were determined from tissue culture supernatants. Cells treated with 0.1% DMSO vehicle were used as an internal control. The EC 50 was calculated as a percentage relative to values obtained with DMSO vehicle-treated cells. Dotted lines indicate 50% inhibition of reporter gene expression (Gluc). Data are expressed as mean and SD from three independent experiments conducted in quadruplicates.

    Article Snippet: To evaluate the effect of the compounds on host protein synthesis, MDCK cells (5.0 × 104 cells/well; 96-well plates; quadruplicates) were transiently transfected, using LPF2000, with 50 ng of a plasmid expressing Gluc under a polymerase II-dependent promoter (pCAGGS-Gluc).

    Techniques: Inhibition, Expressing, Transfection, Plasmid Preparation, Concentration Assay

    Inhibition of viral replication and transcription. Human 293T cells (96-well plate format; 5.0 × 10 4 cells/well; quadruplicates) were transiently transfected with 125 ng of ambisense pDZ plasmids encoding PR8 PB2, PB1, PA, and NP, together with 250 ng of hpPol-I Gluc and hpPol-I GFP vRNA-like expression plasmids and 50 ng of pCAGGS-Cluc. Cells transfected in the absence of pDZ-PB2 were used as a negative control. After 6 h, transfection medium was replaced with medium containing 3-fold serial dilutions of the indicated compounds (starting concentration of 50 μM). At 24 h posttransfection, Gluc and Cluc expression levels were determined from tissue culture supernatants from transfected cells. Transfected cells were also imaged for GFP expression using a fluorescence microscope. Cells treated with 0.1% DMSO vehicle were used as an internal control. The EC 50 was calculated as a percentage relative to values obtained with DMSO vehicle-treated cells. Dotted lines indicate 50% inhibition viral replication and transcription. Data are expressed as mean and SD from three independent experiments conducted in quadruplicate. Bar, 50 μm.

    Journal: Journal of Virology

    Article Title: Identification and Characterization of Novel Compounds with Broad-Spectrum Antiviral Activity against Influenza A and B Viruses

    doi: 10.1128/JVI.02149-19

    Figure Lengend Snippet: Inhibition of viral replication and transcription. Human 293T cells (96-well plate format; 5.0 × 10 4 cells/well; quadruplicates) were transiently transfected with 125 ng of ambisense pDZ plasmids encoding PR8 PB2, PB1, PA, and NP, together with 250 ng of hpPol-I Gluc and hpPol-I GFP vRNA-like expression plasmids and 50 ng of pCAGGS-Cluc. Cells transfected in the absence of pDZ-PB2 were used as a negative control. After 6 h, transfection medium was replaced with medium containing 3-fold serial dilutions of the indicated compounds (starting concentration of 50 μM). At 24 h posttransfection, Gluc and Cluc expression levels were determined from tissue culture supernatants from transfected cells. Transfected cells were also imaged for GFP expression using a fluorescence microscope. Cells treated with 0.1% DMSO vehicle were used as an internal control. The EC 50 was calculated as a percentage relative to values obtained with DMSO vehicle-treated cells. Dotted lines indicate 50% inhibition viral replication and transcription. Data are expressed as mean and SD from three independent experiments conducted in quadruplicate. Bar, 50 μm.

    Article Snippet: To evaluate the effect of the compounds on host protein synthesis, MDCK cells (5.0 × 104 cells/well; 96-well plates; quadruplicates) were transiently transfected, using LPF2000, with 50 ng of a plasmid expressing Gluc under a polymerase II-dependent promoter (pCAGGS-Gluc).

    Techniques: Inhibition, Transfection, Expressing, Negative Control, Concentration Assay, Fluorescence, Microscopy