Monarch Gdna Blood Lysis Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Efficient genome editing in multiple salmonid cell lines using ribonucleoprotein complexes"
Article Title: Efficient genome editing in multiple salmonid cell lines using ribonucleoprotein complexes
Figure Legend Snippet: SHK-1 cells were electroporated with 1.4 μM Cas9:gRNA RNP and transferred to 2 separate 96-well plates. (A) After 48h, cell survival (plate1) was calculated using CellTiter Glo 2.0. (B) genomic DNA (plate 2) was extracted at 7 dpt, and the target sequence amplified by PCR and editing efficiency estimated using Sanger sequencing. (C) Using 1.4 μM RNP, the editing efficiency after 7 and 14 ddpt was estimated for different electroporation settings. (D) All the sequencing data, obtained from ICE analysis of Sanger sequencing of the intergenic target region from optimisation experiments (n=55) were pooled and plotted according to edit pattern.
Techniques Used: Sequencing, Amplification, Polymerase Chain Reaction, Electroporation
2) Product Images from "Functional characterization of the idtF and idtP genes in the Claviceps paspali indole diterpene biosynthetic gene cluster"
Article Title: Functional characterization of the idtF and idtP genes in the Claviceps paspali indole diterpene biosynthetic gene cluster
Journal: Folia Microbiologica
Figure Legend Snippet: Construction of the pAg- idtF -KO and pAg- idtP -KO vectors. The left and right targeting arms (LTA and RTA, respectively) for the idtF and idtP genes, respectively, were PCR amplified from the genomic DNA of the C. paspali DSM833 strain. The backbone of the pAg-H3 plasmid (without the hph gene) and the hph gene alone were amplified separately and fused with the appropriate LTA and RTA amplicons using Gibson assembly. Gene symbols: hph , hygromycin phosphotransferase; nptII : neomycin phosphotransferase II (kanamycin resistance); trfA , plasmid replication initiation gene; oriV, vegetative origin of replication; LB and RB, left and right T-DNA border, respectively
Techniques Used: Polymerase Chain Reaction, Amplification, Plasmid Preparation