monarch dna gel extraction kit (New England Biolabs)
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Name:
Monarch DNA Gel Extraction Kit
Description:
Monarch DNA Gel Extraction Kit 250 preps
Catalog Number:
t1020l
Price:
460
Size:
250 preps
Category:
DNA Fragment Purification from Gels
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New England Biolabs
monarch dna gel extraction kit
Monarch DNA Gel Extraction Kit 250 preps
https://www.bioz.com/result/monarch dna gel extraction kit/product/New England Biolabs
Average 90 stars, based on 77 article reviews
Price from $9.99 to $1999.99
Monarch DNA Gel Extraction Kit 250 preps
https://www.bioz.com/result/monarch dna gel extraction kit/product/New England Biolabs
Average 90 stars, based on 77 article reviews
Price from $9.99 to $1999.99
monarch dna gel extraction kit - by Bioz Stars,
2020-01
90/100 stars
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Images
1) Product Images from "Bordetella pseudohinzii targets cilia and impairs tracheal cilia-driven transport in naturally acquired infection in mice"
Article Title: Bordetella pseudohinzii targets cilia and impairs tracheal cilia-driven transport in naturally acquired infection in mice
Journal: Scientific Reports
doi: 10.1038/s41598-018-23830-4
Figure Legend Snippet: Detection of genomic Bordetella DNA by PCR. ( a ) A PCR product of 318 bp was amplified with B. pseudohinzii -specific primers from DNA of faeces (upper panel) and larynx (lower panel) from infected (lanes 1–4) and non-infected (lanes 5–8) mice, as classified by MALDI-TOF MS. DNA isolated from cultured B. pseudohinzii (strain 3227) served as a positive control (lane 9). Control runs without template were negative (lane 10). M: 100 bp molecular weight marker. ( b ) Trachea and lung taken were taken from 6 animals which were positive for B. pseudohinzii .
Techniques Used: Polymerase Chain Reaction, Amplification, Infection, Mouse Assay, Mass Spectrometry, Isolation, Cell Culture, Positive Control, Molecular Weight, Marker
2) Product Images from "Bordetella pseudohinzii targets cilia and impairs tracheal cilia-driven transport in naturally acquired infection in mice"
Article Title: Bordetella pseudohinzii targets cilia and impairs tracheal cilia-driven transport in naturally acquired infection in mice
Journal: Scientific Reports
doi: 10.1038/s41598-018-23830-4
Figure Legend Snippet: Detection of genomic Bordetella DNA by PCR. ( a ) A PCR product of 318 bp was amplified with B. pseudohinzii -specific primers from DNA of faeces (upper panel) and larynx (lower panel) from infected (lanes 1–4) and non-infected (lanes 5–8) mice, as classified by MALDI-TOF MS. DNA isolated from cultured B. pseudohinzii (strain 3227) served as a positive control (lane 9). Control runs without template were negative (lane 10). M: 100 bp molecular weight marker. ( b ) Trachea and lung taken were taken from 6 animals which were positive for B. pseudohinzii by PCR of faecal pellets, homogenized, and plated on selective agar plates. Number of colony forming units (CFU) is given per 100 mg of tissue. Individual data points shown; horizontal bar and whiskers indicate mean and standard error of the mean. Weight of tissue samples and CFU/organ are provided in Supplementary Table 1 .
Techniques Used: Polymerase Chain Reaction, Amplification, Infection, Mouse Assay, Mass Spectrometry, Isolation, Cell Culture, Positive Control, Molecular Weight, Marker
3) Product Images from "Filter paper-based spin column method for cost-efficient DNA or RNA purification"
Article Title: Filter paper-based spin column method for cost-efficient DNA or RNA purification
Journal: PLoS ONE
doi: 10.1371/journal.pone.0203011
Figure Legend Snippet: The efficiency of filter paper for purification of nucleic acids from various sources using respective Qiagen kits. (A) Tomato genomic DNAs purified using Qiagen DNeasy plant mini kit. (B) Tomato total RNAs purified using Qiagen RNeasy plant mini kit. (C) PCR products of a GUS fragment purified using Qiagen QIAquick PCR purification kit. (D) PCR products of GUS fragment recovered from an agarose gel using a Qiagen QIAquick gel extraction kit. (E) pUC -19 plasmid DNAs purified using a Qiagen QIAprep spin miniprep kit. For each panel, from left to right are (Q) nucleic acid purified in experiments using original Qiagen spin column, (G) reassembled spin column using two layers of Whatman glass microfiber filters (Grade GF/F), and (P) reassembled spin column using two layers of Whatman qualitative filter paper, (Grade 3) respectively. Upper panel is quantification data based on three experimental replicates normalized according to performance of the Qiagen kit; lower panel is an image of agarose gel electrophoresis for the same volume of purified nucleic acids.
Techniques Used: Purification, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Gel Extraction, Plasmid Preparation
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Methylation Sequencing:Article Title: MYC paralog-dependent apoptotic priming orchestrates a spectrum of vulnerabilities in small cell lung cancer Article Snippet: Paragraph title: Bisulfite sequencing ... PCR product was resolved on a 2% agarose gel and purified using the Clone Assay:Article Title: Chromatic Bacteria – A Broad Host-Range Plasmid and Chromosomal Insertion Toolbox for Fluorescent Protein Expression in Bacteria Article Snippet: The amplicons were gel purified using the Article Title: MYC paralog-dependent apoptotic priming orchestrates a spectrum of vulnerabilities in small cell lung cancer Article Snippet: .. PCR product was resolved on a 2% agarose gel and purified using the Article Title: Genotypic characterisation of monepantel resistance in historical and newly derived field strains of Teladorsagia circumcincta Article Snippet: The amplicons were confirmed with gel electrophoresis and cDNA was purified from excised gel slices using the Amplification:Article Title: Chromatic Bacteria – A Broad Host-Range Plasmid and Chromosomal Insertion Toolbox for Fluorescent Protein Expression in Bacteria Article Snippet: To construct the pMRE-Tn7 family, fluorescent protein genes and antibiotic resistance genes were amplified from the pMRE1XX series using primers FWD_pMRE-Tn7 and REV_pMRE-Tn7. .. The amplicons were gel purified using the Article Title: Functional genomics identifies predictive markers and clinically actionable resistance mechanisms to CDK4/6 inhibition in bladder cancer Article Snippet: For the verification of amplified SAM gRNA library, 10 ng of input plasmid DNA was used. .. This was subjected to electrophoresis on a 2% agarose gel and the band of interest was extracted using Article Title: Investigation of Wolbachia spp. and Spiroplasma spp. in Phlebotomus species by molecular methods Article Snippet: .. The amplified DNA fragments of the correct size were excised from the agarose gel and extracted using the Article Title: Genotypic characterisation of monepantel resistance in historical and newly derived field strains of Teladorsagia circumcincta Article Snippet: The Tci-mptl-1 gene was amplified in two sections using a nested-PCR approach: 5′ section amplified with MPTL-SL1 (5′-ATTACCCAAGTTTGAGATCTCAAGG-3′) and MPTL-degR1 (5′-TTTTGCGCATAGGCYTGYTTCAT-3′), followed with semi-nested-PCR with primer pair MTPL-SL1 and MPTL-degR2 (5′-CATRAGAAGCGGCATCTCCAT-3′); the 3′ section was initially amplified with primer pair MPTL-F2 (5′-TGGTCATATACGTACAGAAGCAGG-3′) and MPTL-degR3 (5′-GGMTCTTCMATAGATTTATRAAAATAC-3′) followed by semi-nested-PCR with primer pair MPTL-F2 and MPTL-degR4 (5′-GTGCAGTCAAACGGAACACTTCG-3′). .. The amplicons were confirmed with gel electrophoresis and cDNA was purified from excised gel slices using the Article Title: Feline bocavirus-1 associated with outbreaks of hemorrhagic enteritis in household cats: potential first evidence of a pathological role, viral tropism and natural genetic recombination Article Snippet: Briefly, the total viral RNA/DNA derived from the lymph nodes of three necropsied cats were individually amplified using a Qiagen OneStep RT-PCR kit (Qiagen GmbH, Hilden, Germany) with a combination of reverse transcriptase and DNA polymerase, and specific primers. .. The PCR products were visualized on 1% (w/w) agarose gel electrophoresis and further purified using a Article Title: All-in-one adeno-associated virus delivery and genome editing by Neisseria meningitidis Cas9 in vivo Article Snippet: In the second-step PCR, equimolar amounts of DNA were amplified with a universal forward primer and an indexed reverse primer using Phusion High Fidelity DNA Polymerase (98 °C, 15 s; 61 °C, 25 s; 72 °C, 18 s; nine cycles) to ligate the TruSeq adaptors. .. The resultant amplicons were separated in a 2.5% agarose gel and the corresponding ~ 250-bp product bands were extracted using Positive Control:Article Title: Investigation of Wolbachia spp. and Spiroplasma spp. in Phlebotomus species by molecular methods Article Snippet: Nuclease free water was used as negative control and female tsetse fly DNA (Glossina fuscipes fuscipes ) obtained from the field in Uganda was used as a positive control. .. The amplified DNA fragments of the correct size were excised from the agarose gel and extracted using the Construct:Article Title: Chromatic Bacteria – A Broad Host-Range Plasmid and Chromosomal Insertion Toolbox for Fluorescent Protein Expression in Bacteria Article Snippet: To construct the pMRE-Tn7 family, fluorescent protein genes and antibiotic resistance genes were amplified from the pMRE1XX series using primers FWD_pMRE-Tn7 and REV_pMRE-Tn7. .. The amplicons were gel purified using the Electrophoresis:Article Title: Functional genomics identifies predictive markers and clinically actionable resistance mechanisms to CDK4/6 inhibition in bladder cancer Article Snippet: .. This was subjected to electrophoresis on a 2% agarose gel and the band of interest was extracted using Article Title: Continuous directed evolution of proteins with improved soluble expression Article Snippet: .. PCR 2 conditions: 98 °C for 2 min, then 12 cycles of (98 °C for 15 s, 61 °C for 20 s, 72 °C for 20 s), followed by a final 72 °C extension for 2 min. PCR products were pooled and purified by electrophoresis with a 2% agarose gel using a Article Title: High-yield fabrication of DNA and RNA constructs for single molecule force and torque spectroscopy experiments Article Snippet: .. When necessary, final products were separated from non-ligated fragments by electrophoresis using a 0.8–1.5% (m/V) agarose gel stained with Sybr™ Gold (Thermo Fisher Scientific) ( ) and extracted from the gel with the Incubation:Article Title: DNA assembly for nanopore data storage readout Article Snippet: PCR reactions were gel-purified using a 1% agarose gel and NEB Cell Culture:Article Title: Bordetella pseudohinzii targets cilia and impairs tracheal cilia-driven transport in naturally acquired infection in mice Article Snippet: Genomic DNA was isolated from faecal and larynx samples (infected and non-infected, each n = 4) and cultured B. pseudohinzii (n = 1) with the ISOLATE Faecal DNA Kit (Bioline, Luckenwalde, Germany) according to the manufacturer’s protocol. .. For subsequent sequencing, PCR-products were purified by the use of the Article Title: Investigation of Wolbachia spp. and Spiroplasma spp. in Phlebotomus species by molecular methods Article Snippet: The amplified DNA fragments of the correct size were excised from the agarose gel and extracted using the Touchdown PCR:Article Title: Genotypic characterisation of monepantel resistance in historical and newly derived field strains of Teladorsagia circumcincta Article Snippet: Amplification was conducted using Q5® high-fidelity DNA polymerase (New England Biolabs) in a final volume of 25 μl (Q5®high-fidelity reaction buffer, 0.2 mM dNTPs, 0.5 μM of each primer, 0.02 U/μl Q5® high-fidelity DNA polymerase and 1 μl template cDNA), using touchdown PCR thermocycling conditions as follows: initial denaturing step at 98 °C for 2 min; 10 three-step cycles of 98 °C for 10 s, Tanneal for 20 s and 72 °C for 90 s, with decreasing annealing temperature by 0.5 °C per cycle from the (Tanneal + 5 °C) to the Tanneal ; this was followed by 30 three-step cycles of 98 °C for 10 s, Tanneal for 20 s and 72 °C for 90 s with a final extension step of 72 °C for 5 min. .. The amplicons were confirmed with gel electrophoresis and cDNA was purified from excised gel slices using the Modification:Article Title: Efficient Genome Editing of Magnetospirillum magneticum AMB-1 by CRISPR-Cas9 System for Analyzing Magnetotactic Behavior Article Snippet: DNA manipulation, plasmid construction and conjugation The enzymes for DNA modification were purchased from New England Biolabs (NEB) and Takara Biomedical Technology. .. All PCR products and plasmids were purified using Transformation Assay:Article Title: Chromatic Bacteria – A Broad Host-Range Plasmid and Chromosomal Insertion Toolbox for Fluorescent Protein Expression in Bacteria Article Snippet: For isothermal assemblies, EcoRV-digested pAG408 was mixed with the insert fragments in a 1:3:3 ratio as described above and assembled plasmids were transformed into chemically competent E. coli S17-1. .. The amplicons were gel purified using the Article Title: MYC paralog-dependent apoptotic priming orchestrates a spectrum of vulnerabilities in small cell lung cancer Article Snippet: .. PCR product was resolved on a 2% agarose gel and purified using the Article Title: Investigation of Wolbachia spp. and Spiroplasma spp. in Phlebotomus species by molecular methods Article Snippet: The amplified DNA fragments of the correct size were excised from the agarose gel and extracted using the Derivative Assay:Article Title: Feline bocavirus-1 associated with outbreaks of hemorrhagic enteritis in household cats: potential first evidence of a pathological role, viral tropism and natural genetic recombination Article Snippet: Briefly, the total viral RNA/DNA derived from the lymph nodes of three necropsied cats were individually amplified using a Qiagen OneStep RT-PCR kit (Qiagen GmbH, Hilden, Germany) with a combination of reverse transcriptase and DNA polymerase, and specific primers. .. The PCR products were visualized on 1% (w/w) agarose gel electrophoresis and further purified using a Gel Purification:Article Title: Darwin Assembly: fast, efficient, multi-site bespoke mutagenesis Article Snippet: .. Gel purification was carried out using Conjugation Assay:Article Title: Efficient Genome Editing of Magnetospirillum magneticum AMB-1 by CRISPR-Cas9 System for Analyzing Magnetotactic Behavior Article Snippet: Paragraph title: DNA manipulation, plasmid construction and conjugation ... All PCR products and plasmids were purified using Sequencing:Article Title: Functional genomics identifies predictive markers and clinically actionable resistance mechanisms to CDK4/6 inhibition in bladder cancer Article Snippet: To control for representation, PCR with gRNAs sequence primer listed in Additional file : Table S1b was performed on genomic DNA equivalent to 500 cells per guide, corresponding to a total of 231 μg from 35 million cells (assuming 6.6 pg in a single diploid cell). .. This was subjected to electrophoresis on a 2% agarose gel and the band of interest was extracted using Article Title: MYC paralog-dependent apoptotic priming orchestrates a spectrum of vulnerabilities in small cell lung cancer Article Snippet: PCR product was resolved on a 2% agarose gel and purified using the Article Title: Continuous directed evolution of proteins with improved soluble expression Article Snippet: Paragraph title: High-throughput sequencing of genomic DNA. ... PCR 2 conditions: 98 °C for 2 min, then 12 cycles of (98 °C for 15 s, 61 °C for 20 s, 72 °C for 20 s), followed by a final 72 °C extension for 2 min. PCR products were pooled and purified by electrophoresis with a 2% agarose gel using a Article Title: Bordetella pseudohinzii targets cilia and impairs tracheal cilia-driven transport in naturally acquired infection in mice Article Snippet: .. For subsequent sequencing, PCR-products were purified by the use of the Article Title: Characterization of Equine Parvovirus in Thoroughbred Breeding Horses from Germany Article Snippet: Paragraph title: 2.5. Sequencing and Phylogeny ... PCR products were visualised on a 2% agarose gel, excised, and purified using a Article Title: Genotypic characterisation of monepantel resistance in historical and newly derived field strains of Teladorsagia circumcincta Article Snippet: The amplicons were confirmed with gel electrophoresis and cDNA was purified from excised gel slices using the Article Title: Feline bocavirus-1 associated with outbreaks of hemorrhagic enteritis in household cats: potential first evidence of a pathological role, viral tropism and natural genetic recombination Article Snippet: .. The PCR products were visualized on 1% (w/w) agarose gel electrophoresis and further purified using a DNA Ligation:Article Title: High-yield fabrication of DNA and RNA constructs for single molecule force and torque spectroscopy experiments Article Snippet: T4 DNA ligase was used for DNA ligations 1h or overnight at 16°C in 1× T4 DNA ligation buffer. .. When necessary, final products were separated from non-ligated fragments by electrophoresis using a 0.8–1.5% (m/V) agarose gel stained with Sybr™ Gold (Thermo Fisher Scientific) ( ) and extracted from the gel with the Ligation:Article Title: Darwin Assembly: fast, efficient, multi-site bespoke mutagenesis Article Snippet: Gel purification was carried out using Article Title: High-yield fabrication of DNA and RNA constructs for single molecule force and torque spectroscopy experiments Article Snippet: Paragraph title: Restriction enzyme digest and ligation ... When necessary, final products were separated from non-ligated fragments by electrophoresis using a 0.8–1.5% (m/V) agarose gel stained with Sybr™ Gold (Thermo Fisher Scientific) ( ) and extracted from the gel with the Infection:Article Title: Bordetella pseudohinzii targets cilia and impairs tracheal cilia-driven transport in naturally acquired infection in mice Article Snippet: Genomic DNA was isolated from faecal and larynx samples (infected and non-infected, each n = 4) and cultured B. pseudohinzii (n = 1) with the ISOLATE Faecal DNA Kit (Bioline, Luckenwalde, Germany) according to the manufacturer’s protocol. .. For subsequent sequencing, PCR-products were purified by the use of the Generated:Article Title: MYC paralog-dependent apoptotic priming orchestrates a spectrum of vulnerabilities in small cell lung cancer Article Snippet: PCR product was resolved on a 2% agarose gel and purified using the Article Title: Feline bocavirus-1 associated with outbreaks of hemorrhagic enteritis in household cats: potential first evidence of a pathological role, viral tropism and natural genetic recombination Article Snippet: The amplicons were generated by thermocycling at 50 °C for 30 min, 94 °C for 15 min and then 40 cycles of 94 °C for 30 s, 50 °C for 30 s and 72 °C for 1 min, before a final 72 °C for 7 min. .. The PCR products were visualized on 1% (w/w) agarose gel electrophoresis and further purified using a DNA Sequencing:Article Title: Investigation of Wolbachia spp. and Spiroplasma spp. in Phlebotomus species by molecular methods Article Snippet: The amplified DNA fragments of the correct size were excised from the agarose gel and extracted using the Polymerase Chain Reaction:Article Title: MYC paralog-dependent apoptotic priming orchestrates a spectrum of vulnerabilities in small cell lung cancer Article Snippet: .. PCR product was resolved on a 2% agarose gel and purified using the Article Title: Efficient Genome Editing of Magnetospirillum magneticum AMB-1 by CRISPR-Cas9 System for Analyzing Magnetotactic Behavior Article Snippet: .. All PCR products and plasmids were purified using Article Title: Continuous directed evolution of proteins with improved soluble expression Article Snippet: .. PCR 2 conditions: 98 °C for 2 min, then 12 cycles of (98 °C for 15 s, 61 °C for 20 s, 72 °C for 20 s), followed by a final 72 °C extension for 2 min. PCR products were pooled and purified by electrophoresis with a 2% agarose gel using a Article Title: Bordetella pseudohinzii targets cilia and impairs tracheal cilia-driven transport in naturally acquired infection in mice Article Snippet: .. For subsequent sequencing, PCR-products were purified by the use of the Article Title: Characterization of Equine Parvovirus in Thoroughbred Breeding Horses from Germany Article Snippet: .. PCR products were visualised on a 2% agarose gel, excised, and purified using a Article Title: Darwin Assembly: fast, efficient, multi-site bespoke mutagenesis Article Snippet: PCR products were purified using GeneJET PCR Purification Kits (Thermo Fisher Scientific, Waltham MA, USA), Nucleospin Gel and PCR Clean-up (Machery-Nagel GmbH, Düren, Germany) or Monarch PCR and DNA Cleanup kits (NEB). .. Gel purification was carried out using Article Title: High-yield fabrication of DNA and RNA constructs for single molecule force and torque spectroscopy experiments Article Snippet: When necessary, final products were separated from non-ligated fragments by electrophoresis using a 0.8–1.5% (m/V) agarose gel stained with Sybr™ Gold (Thermo Fisher Scientific) ( ) and extracted from the gel with the Article Title: Investigation of Wolbachia spp. and Spiroplasma spp. in Phlebotomus species by molecular methods Article Snippet: The resulting PCR products were analyzed by agarose gel-electrophoresis on a 1% gel, stained with ethidium bromide and recorded using the Gel Doc EQ quantification analysis software (Bio-Rad, Image Lab Software Version 4.1). .. The amplified DNA fragments of the correct size were excised from the agarose gel and extracted using the Article Title: Genotypic characterisation of monepantel resistance in historical and newly derived field strains of Teladorsagia circumcincta Article Snippet: The amplicons were confirmed with gel electrophoresis and cDNA was purified from excised gel slices using the Article Title: Feline bocavirus-1 associated with outbreaks of hemorrhagic enteritis in household cats: potential first evidence of a pathological role, viral tropism and natural genetic recombination Article Snippet: .. The PCR products were visualized on 1% (w/w) agarose gel electrophoresis and further purified using a Article Title: DNA assembly for nanopore data storage readout Article Snippet: .. PCR reactions were gel-purified using a 1% agarose gel and NEB Article Title: All-in-one adeno-associated virus delivery and genome editing by Neisseria meningitidis Cas9 in vivo Article Snippet: In the second-step PCR, equimolar amounts of DNA were amplified with a universal forward primer and an indexed reverse primer using Phusion High Fidelity DNA Polymerase (98 °C, 15 s; 61 °C, 25 s; 72 °C, 18 s; nine cycles) to ligate the TruSeq adaptors. .. The resultant amplicons were separated in a 2.5% agarose gel and the corresponding ~ 250-bp product bands were extracted using Hybridization:Article Title: High-yield fabrication of DNA and RNA constructs for single molecule force and torque spectroscopy experiments Article Snippet: When necessary, final products were separated from non-ligated fragments by electrophoresis using a 0.8–1.5% (m/V) agarose gel stained with Sybr™ Gold (Thermo Fisher Scientific) ( ) and extracted from the gel with the Multiplexing:Article Title: Functional genomics identifies predictive markers and clinically actionable resistance mechanisms to CDK4/6 inhibition in bladder cancer Article Snippet: PCR was conducted using Phusion Flash High-Fidelity PCR master mix (Life Technologies) for 28 cycles as 98 °C for 90 s, 98 °C for 1 s, 60 °C for 5 s, 72 °C for 15 s, followed by final extension of 72 °C for 1 min. To enable multiplexing during NGS, an 8 bp unique barcode was added at the beginning of the forward primer as TCGCCTTG, ATAGCGTC, GAAGAAGT, ATTCTAGG or CGTTACCA. .. This was subjected to electrophoresis on a 2% agarose gel and the band of interest was extracted using Nucleic Acid Electrophoresis:Article Title: Genotypic characterisation of monepantel resistance in historical and newly derived field strains of Teladorsagia circumcincta Article Snippet: .. The amplicons were confirmed with gel electrophoresis and cDNA was purified from excised gel slices using the Methylation:Article Title: MYC paralog-dependent apoptotic priming orchestrates a spectrum of vulnerabilities in small cell lung cancer Article Snippet: Bisulfite-converted DNA was subjected to methylation-specific PCR using specific primers for the BCL2 promoter listed in Supplementary Table . .. PCR product was resolved on a 2% agarose gel and purified using the Mutagenesis:Article Title: Darwin Assembly: fast, efficient, multi-site bespoke mutagenesis Article Snippet: Gel purification was carried out using Isolation:Article Title: Bordetella pseudohinzii targets cilia and impairs tracheal cilia-driven transport in naturally acquired infection in mice Article Snippet: Genomic DNA was isolated from faecal and larynx samples (infected and non-infected, each n = 4) and cultured B. pseudohinzii (n = 1) with the ISOLATE Faecal DNA Kit (Bioline, Luckenwalde, Germany) according to the manufacturer’s protocol. .. For subsequent sequencing, PCR-products were purified by the use of the Marker:Article Title: Chromatic Bacteria – A Broad Host-Range Plasmid and Chromosomal Insertion Toolbox for Fluorescent Protein Expression in Bacteria Article Snippet: To construct the pMRE-Tn5 plasmid series, pMRE1XX plasmids were used as a template to amplify the desired DNA fragments, which include one of the eight fluorescent protein gene and one of the four antibiotic resistance marker combinations. .. The amplicons were gel purified using the Multiplex Assay:Article Title: Continuous directed evolution of proteins with improved soluble expression Article Snippet: PCR 2 reactions used 1.25 μL each of 10 μM forward and reverse Illumina barcoding primers and 1 μL of unpurified PCR 1 reaction product, all diluted to 12.5 μL with nuclease-free water, and 12.5 μL Phusion U Green Multiplex PCR MasterMix (Thermo Fisher Scientific). .. PCR 2 conditions: 98 °C for 2 min, then 12 cycles of (98 °C for 15 s, 61 °C for 20 s, 72 °C for 20 s), followed by a final 72 °C extension for 2 min. PCR products were pooled and purified by electrophoresis with a 2% agarose gel using a Purification:Article Title: Chromatic Bacteria – A Broad Host-Range Plasmid and Chromosomal Insertion Toolbox for Fluorescent Protein Expression in Bacteria Article Snippet: .. The amplicons were gel purified using the Article Title: MYC paralog-dependent apoptotic priming orchestrates a spectrum of vulnerabilities in small cell lung cancer Article Snippet: .. PCR product was resolved on a 2% agarose gel and purified using the Article Title: Efficient Genome Editing of Magnetospirillum magneticum AMB-1 by CRISPR-Cas9 System for Analyzing Magnetotactic Behavior Article Snippet: .. All PCR products and plasmids were purified using Article Title: Continuous directed evolution of proteins with improved soluble expression Article Snippet: .. PCR 2 conditions: 98 °C for 2 min, then 12 cycles of (98 °C for 15 s, 61 °C for 20 s, 72 °C for 20 s), followed by a final 72 °C extension for 2 min. PCR products were pooled and purified by electrophoresis with a 2% agarose gel using a Article Title: Bordetella pseudohinzii targets cilia and impairs tracheal cilia-driven transport in naturally acquired infection in mice Article Snippet: .. For subsequent sequencing, PCR-products were purified by the use of the Article Title: Characterization of Equine Parvovirus in Thoroughbred Breeding Horses from Germany Article Snippet: .. PCR products were visualised on a 2% agarose gel, excised, and purified using a Article Title: Darwin Assembly: fast, efficient, multi-site bespoke mutagenesis Article Snippet: PCR products were purified using GeneJET PCR Purification Kits (Thermo Fisher Scientific, Waltham MA, USA), Nucleospin Gel and PCR Clean-up (Machery-Nagel GmbH, Düren, Germany) or Monarch PCR and DNA Cleanup kits (NEB). .. Gel purification was carried out using Article Title: Investigation of Wolbachia spp. and Spiroplasma spp. in Phlebotomus species by molecular methods Article Snippet: The amplified DNA fragments of the correct size were excised from the agarose gel and extracted using the Article Title: Genotypic characterisation of monepantel resistance in historical and newly derived field strains of Teladorsagia circumcincta Article Snippet: .. The amplicons were confirmed with gel electrophoresis and cDNA was purified from excised gel slices using the Article Title: Feline bocavirus-1 associated with outbreaks of hemorrhagic enteritis in household cats: potential first evidence of a pathological role, viral tropism and natural genetic recombination Article Snippet: .. The PCR products were visualized on 1% (w/w) agarose gel electrophoresis and further purified using a Article Title: DNA assembly for nanopore data storage readout Article Snippet: PCR reactions were gel-purified using a 1% agarose gel and NEB Article Title: High-yield fabrication of DNA and RNA constructs for single molecule force and torque spectroscopy experiments Article Snippet: .. The ssDNA fragments were identified using a blue light transilluminator , cut out of the gel and purified using the Reverse Transcription Polymerase Chain Reaction:Article Title: Feline bocavirus-1 associated with outbreaks of hemorrhagic enteritis in household cats: potential first evidence of a pathological role, viral tropism and natural genetic recombination Article Snippet: Briefly, the total viral RNA/DNA derived from the lymph nodes of three necropsied cats were individually amplified using a Qiagen OneStep RT-PCR kit (Qiagen GmbH, Hilden, Germany) with a combination of reverse transcriptase and DNA polymerase, and specific primers. .. The PCR products were visualized on 1% (w/w) agarose gel electrophoresis and further purified using a Selection:Article Title: MYC paralog-dependent apoptotic priming orchestrates a spectrum of vulnerabilities in small cell lung cancer Article Snippet: .. PCR product was resolved on a 2% agarose gel and purified using the De-Phosphorylation Assay:Article Title: Chromatic Bacteria – A Broad Host-Range Plasmid and Chromosomal Insertion Toolbox for Fluorescent Protein Expression in Bacteria Article Snippet: The amplicons were gel purified using the Gel Extraction:Article Title: Chromatic Bacteria – A Broad Host-Range Plasmid and Chromosomal Insertion Toolbox for Fluorescent Protein Expression in Bacteria Article Snippet: .. The amplicons were gel purified using the Article Title: Functional genomics identifies predictive markers and clinically actionable resistance mechanisms to CDK4/6 inhibition in bladder cancer Article Snippet: .. This was subjected to electrophoresis on a 2% agarose gel and the band of interest was extracted using Article Title: MYC paralog-dependent apoptotic priming orchestrates a spectrum of vulnerabilities in small cell lung cancer Article Snippet: .. PCR product was resolved on a 2% agarose gel and purified using the Article Title: Efficient Genome Editing of Magnetospirillum magneticum AMB-1 by CRISPR-Cas9 System for Analyzing Magnetotactic Behavior Article Snippet: .. All PCR products and plasmids were purified using Article Title: Continuous directed evolution of proteins with improved soluble expression Article Snippet: .. PCR 2 conditions: 98 °C for 2 min, then 12 cycles of (98 °C for 15 s, 61 °C for 20 s, 72 °C for 20 s), followed by a final 72 °C extension for 2 min. PCR products were pooled and purified by electrophoresis with a 2% agarose gel using a Article Title: Bordetella pseudohinzii targets cilia and impairs tracheal cilia-driven transport in naturally acquired infection in mice Article Snippet: .. For subsequent sequencing, PCR-products were purified by the use of the Article Title: Characterization of Equine Parvovirus in Thoroughbred Breeding Horses from Germany Article Snippet: .. PCR products were visualised on a 2% agarose gel, excised, and purified using a Article Title: Darwin Assembly: fast, efficient, multi-site bespoke mutagenesis Article Snippet: .. Gel purification was carried out using Article Title: High-yield fabrication of DNA and RNA constructs for single molecule force and torque spectroscopy experiments Article Snippet: .. When necessary, final products were separated from non-ligated fragments by electrophoresis using a 0.8–1.5% (m/V) agarose gel stained with Sybr™ Gold (Thermo Fisher Scientific) ( ) and extracted from the gel with the Article Title: Investigation of Wolbachia spp. and Spiroplasma spp. in Phlebotomus species by molecular methods Article Snippet: .. The amplified DNA fragments of the correct size were excised from the agarose gel and extracted using the Article Title: Genotypic characterisation of monepantel resistance in historical and newly derived field strains of Teladorsagia circumcincta Article Snippet: .. The amplicons were confirmed with gel electrophoresis and cDNA was purified from excised gel slices using the Article Title: Feline bocavirus-1 associated with outbreaks of hemorrhagic enteritis in household cats: potential first evidence of a pathological role, viral tropism and natural genetic recombination Article Snippet: .. The PCR products were visualized on 1% (w/w) agarose gel electrophoresis and further purified using a Article Title: DNA assembly for nanopore data storage readout Article Snippet: .. PCR reactions were gel-purified using a 1% agarose gel and NEB Article Title: High-yield fabrication of DNA and RNA constructs for single molecule force and torque spectroscopy experiments Article Snippet: .. The ssDNA fragments were identified using a blue light transilluminator , cut out of the gel and purified using the Article Title: All-in-one adeno-associated virus delivery and genome editing by Neisseria meningitidis Cas9 in vivo Article Snippet: .. The resultant amplicons were separated in a 2.5% agarose gel and the corresponding ~ 250-bp product bands were extracted using Nested PCR:Article Title: Genotypic characterisation of monepantel resistance in historical and newly derived field strains of Teladorsagia circumcincta Article Snippet: The Tci-mptl-1 gene was amplified in two sections using a nested-PCR approach: 5′ section amplified with MPTL-SL1 (5′-ATTACCCAAGTTTGAGATCTCAAGG-3′) and MPTL-degR1 (5′-TTTTGCGCATAGGCYTGYTTCAT-3′), followed with semi-nested-PCR with primer pair MTPL-SL1 and MPTL-degR2 (5′-CATRAGAAGCGGCATCTCCAT-3′); the 3′ section was initially amplified with primer pair MPTL-F2 (5′-TGGTCATATACGTACAGAAGCAGG-3′) and MPTL-degR3 (5′-GGMTCTTCMATAGATTTATRAAAATAC-3′) followed by semi-nested-PCR with primer pair MPTL-F2 and MPTL-degR4 (5′-GTGCAGTCAAACGGAACACTTCG-3′). .. The amplicons were confirmed with gel electrophoresis and cDNA was purified from excised gel slices using the Polymerase Cycling Assembly:Article Title: DNA assembly for nanopore data storage readout Article Snippet: Gibson assembly PCR reactions were cleaned-up using KAPA Pure beads (Roche Cat # KK8000) before added to the first Gibson Assembly. .. PCR reactions were gel-purified using a 1% agarose gel and NEB Plasmid Preparation:Article Title: Chromatic Bacteria – A Broad Host-Range Plasmid and Chromosomal Insertion Toolbox for Fluorescent Protein Expression in Bacteria Article Snippet: Paragraph title: Plasmid Construction ... The amplicons were gel purified using the Article Title: Functional genomics identifies predictive markers and clinically actionable resistance mechanisms to CDK4/6 inhibition in bladder cancer Article Snippet: For the verification of amplified SAM gRNA library, 10 ng of input plasmid DNA was used. .. This was subjected to electrophoresis on a 2% agarose gel and the band of interest was extracted using Article Title: MYC paralog-dependent apoptotic priming orchestrates a spectrum of vulnerabilities in small cell lung cancer Article Snippet: .. PCR product was resolved on a 2% agarose gel and purified using the Article Title: Efficient Genome Editing of Magnetospirillum magneticum AMB-1 by CRISPR-Cas9 System for Analyzing Magnetotactic Behavior Article Snippet: .. All PCR products and plasmids were purified using Article Title: Investigation of Wolbachia spp. and Spiroplasma spp. in Phlebotomus species by molecular methods Article Snippet: The amplified DNA fragments of the correct size were excised from the agarose gel and extracted using the Article Title: Genotypic characterisation of monepantel resistance in historical and newly derived field strains of Teladorsagia circumcincta Article Snippet: The amplicons were confirmed with gel electrophoresis and cDNA was purified from excised gel slices using the Software:Article Title: Investigation of Wolbachia spp. and Spiroplasma spp. in Phlebotomus species by molecular methods Article Snippet: The resulting PCR products were analyzed by agarose gel-electrophoresis on a 1% gel, stained with ethidium bromide and recorded using the Gel Doc EQ quantification analysis software (Bio-Rad, Image Lab Software Version 4.1). .. The amplified DNA fragments of the correct size were excised from the agarose gel and extracted using the Article Title: Genotypic characterisation of monepantel resistance in historical and newly derived field strains of Teladorsagia circumcincta Article Snippet: The amplicons were confirmed with gel electrophoresis and cDNA was purified from excised gel slices using the Negative Control:Article Title: Investigation of Wolbachia spp. and Spiroplasma spp. in Phlebotomus species by molecular methods Article Snippet: Nuclease free water was used as negative control and female tsetse fly DNA (Glossina fuscipes fuscipes ) obtained from the field in Uganda was used as a positive control. .. The amplified DNA fragments of the correct size were excised from the agarose gel and extracted using the Recombinant:Article Title: Investigation of Wolbachia spp. and Spiroplasma spp. in Phlebotomus species by molecular methods Article Snippet: The amplified DNA fragments of the correct size were excised from the agarose gel and extracted using the Agarose Gel Electrophoresis:Article Title: Functional genomics identifies predictive markers and clinically actionable resistance mechanisms to CDK4/6 inhibition in bladder cancer Article Snippet: .. This was subjected to electrophoresis on a 2% agarose gel and the band of interest was extracted using Article Title: MYC paralog-dependent apoptotic priming orchestrates a spectrum of vulnerabilities in small cell lung cancer Article Snippet: .. PCR product was resolved on a 2% agarose gel and purified using the Article Title: Continuous directed evolution of proteins with improved soluble expression Article Snippet: .. PCR 2 conditions: 98 °C for 2 min, then 12 cycles of (98 °C for 15 s, 61 °C for 20 s, 72 °C for 20 s), followed by a final 72 °C extension for 2 min. PCR products were pooled and purified by electrophoresis with a 2% agarose gel using a Article Title: Characterization of Equine Parvovirus in Thoroughbred Breeding Horses from Germany Article Snippet: .. PCR products were visualised on a 2% agarose gel, excised, and purified using a Article Title: High-yield fabrication of DNA and RNA constructs for single molecule force and torque spectroscopy experiments Article Snippet: .. When necessary, final products were separated from non-ligated fragments by electrophoresis using a 0.8–1.5% (m/V) agarose gel stained with Sybr™ Gold (Thermo Fisher Scientific) ( ) and extracted from the gel with the Article Title: Investigation of Wolbachia spp. and Spiroplasma spp. in Phlebotomus species by molecular methods Article Snippet: .. The amplified DNA fragments of the correct size were excised from the agarose gel and extracted using the Article Title: Feline bocavirus-1 associated with outbreaks of hemorrhagic enteritis in household cats: potential first evidence of a pathological role, viral tropism and natural genetic recombination Article Snippet: .. The PCR products were visualized on 1% (w/w) agarose gel electrophoresis and further purified using a Article Title: DNA assembly for nanopore data storage readout Article Snippet: .. PCR reactions were gel-purified using a 1% agarose gel and NEB Article Title: All-in-one adeno-associated virus delivery and genome editing by Neisseria meningitidis Cas9 in vivo Article Snippet: .. The resultant amplicons were separated in a 2.5% agarose gel and the corresponding ~ 250-bp product bands were extracted using Next-Generation Sequencing:Article Title: Functional genomics identifies predictive markers and clinically actionable resistance mechanisms to CDK4/6 inhibition in bladder cancer Article Snippet: Paragraph title: Genomic DNA extraction and next-generation sequencing (NGS) ... This was subjected to electrophoresis on a 2% agarose gel and the band of interest was extracted using DNA Methylation Assay:Article Title: MYC paralog-dependent apoptotic priming orchestrates a spectrum of vulnerabilities in small cell lung cancer Article Snippet: PCR product was resolved on a 2% agarose gel and purified using the DNA Extraction:Article Title: Functional genomics identifies predictive markers and clinically actionable resistance mechanisms to CDK4/6 inhibition in bladder cancer Article Snippet: Paragraph title: Genomic DNA extraction and next-generation sequencing (NGS) ... This was subjected to electrophoresis on a 2% agarose gel and the band of interest was extracted using Article Title: Bordetella pseudohinzii targets cilia and impairs tracheal cilia-driven transport in naturally acquired infection in mice Article Snippet: Paragraph title: DNA isolation and PCR ... For subsequent sequencing, PCR-products were purified by the use of the Concentration Assay:Article Title: Continuous directed evolution of proteins with improved soluble expression Article Snippet: .. PCR 2 conditions: 98 °C for 2 min, then 12 cycles of (98 °C for 15 s, 61 °C for 20 s, 72 °C for 20 s), followed by a final 72 °C extension for 2 min. PCR products were pooled and purified by electrophoresis with a 2% agarose gel using a High Throughput Screening Assay:Article Title: Continuous directed evolution of proteins with improved soluble expression Article Snippet: Paragraph title: High-throughput sequencing of genomic DNA. ... PCR 2 conditions: 98 °C for 2 min, then 12 cycles of (98 °C for 15 s, 61 °C for 20 s, 72 °C for 20 s), followed by a final 72 °C extension for 2 min. PCR products were pooled and purified by electrophoresis with a 2% agarose gel using a Staining:Article Title: High-yield fabrication of DNA and RNA constructs for single molecule force and torque spectroscopy experiments Article Snippet: .. When necessary, final products were separated from non-ligated fragments by electrophoresis using a 0.8–1.5% (m/V) agarose gel stained with Sybr™ Gold (Thermo Fisher Scientific) ( ) and extracted from the gel with the Article Title: Investigation of Wolbachia spp. and Spiroplasma spp. in Phlebotomus species by molecular methods Article Snippet: The resulting PCR products were analyzed by agarose gel-electrophoresis on a 1% gel, stained with ethidium bromide and recorded using the Gel Doc EQ quantification analysis software (Bio-Rad, Image Lab Software Version 4.1). .. The amplified DNA fragments of the correct size were excised from the agarose gel and extracted using the |