gdna (New England Biolabs)
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Name:
Monarch Genomic DNA Purification Kit
Description:
The Monarch Genomic DNA Purification Kit is a comprehensive solution for cell lysis RNA removal and purification of intact genomic DNA gDNA from a wide variety of biological samples including cultured cells blood and mammalian tissues Additionally bacteria and yeast can be processed with extra steps to enhance lysis in these tough to lyse samples Protocols are also included to enable purification from clinically relevant samples such as saliva and cheek swabs as well as rapid cleanup of previously extracted gDNA Purified gDNA has high quality metrics including A260 A280 1 8 and A260 A230 2 0 high DIN scores and minimal residual RNA The purified gDNA is suitable for downstream applications such as end point PCR qPCR and library prep for NGS It typically has a peak size of 50kb making this kit an excellent choice upstream of long read sequencing platforms
Catalog Number:
T3010L
Price:
395
Category:
Genomic DNA Purification Kits
Size:
150 preps
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Buy from Supplier |
Structured Review
New England Biolabs
gdna
The Monarch Genomic DNA Purification Kit is a comprehensive solution for cell lysis RNA removal and purification of intact genomic DNA gDNA from a wide variety of biological samples including cultured cells blood and mammalian tissues Additionally bacteria and yeast can be processed with extra steps to enhance lysis in these tough to lyse samples Protocols are also included to enable purification from clinically relevant samples such as saliva and cheek swabs as well as rapid cleanup of previously extracted gDNA Purified gDNA has high quality metrics including A260 A280 1 8 and A260 A230 2 0 high DIN scores and minimal residual RNA The purified gDNA is suitable for downstream applications such as end point PCR qPCR and library prep for NGS It typically has a peak size of 50kb making this kit an excellent choice upstream of long read sequencing platforms
https://www.bioz.com/result/gdna/product/New England Biolabs
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
The Monarch Genomic DNA Purification Kit is a comprehensive solution for cell lysis RNA removal and purification of intact genomic DNA gDNA from a wide variety of biological samples including cultured cells blood and mammalian tissues Additionally bacteria and yeast can be processed with extra steps to enhance lysis in these tough to lyse samples Protocols are also included to enable purification from clinically relevant samples such as saliva and cheek swabs as well as rapid cleanup of previously extracted gDNA Purified gDNA has high quality metrics including A260 A280 1 8 and A260 A230 2 0 high DIN scores and minimal residual RNA The purified gDNA is suitable for downstream applications such as end point PCR qPCR and library prep for NGS It typically has a peak size of 50kb making this kit an excellent choice upstream of long read sequencing platforms
https://www.bioz.com/result/gdna/product/New England Biolabs
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
gdna - by Bioz Stars,
2021-04
99/100 stars
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Amplification:Article Title: Bacterial defenses against a natural antibiotic promote collateral resilience to clinical antibiotics Article Snippet: Briefly, for unmarked deletions, ∼1kb fragments immediately upstream and downstream of the target locus were cloned using Gibson assembly into the pMQ30 suicide vector , . .. Fragments amplified from P. aeruginosa PA14 genomic DNA (gDNA) and cleaned up using the Article Title: A High-Efficacy CRISPR Interference System for Gene Function Discovery in Zymomonas mobilis Article Snippet: Oligonucleotides (2 μM each) were annealed in 1× CutSmart buffer (NEB) at 95°C for 5 min, followed by cooling to room temperature. .. For method two, fragments were amplified by PCR with primers oJMP197 and oJMP198 from a 78-nt oligonucleotide, followed by digestion with BsaI-HF-v2 (catalog number R3733; NEB) and purification with the Article Title: Bacterial defenses against a natural antibiotic promote collateral resilience to clinical antibiotics Article Snippet: Briefly, for unmarked deletions, approximately 1-kb fragments immediately upstream and downstream of the target locus were cloned using Gibson assembly into the pMQ30 suicide vector [ , ]. .. Fragments amplified from P . aeruginosa PA14 genomic DNA (gDNA) and cleaned up using the Polymerase Chain Reaction:Article Title: Bacterial defenses against a natural antibiotic promote collateral resilience to clinical antibiotics Article Snippet: Briefly, for unmarked deletions, ∼1kb fragments immediately upstream and downstream of the target locus were cloned using Gibson assembly into the pMQ30 suicide vector , . .. Fragments amplified from P. aeruginosa PA14 genomic DNA (gDNA) and cleaned up using the Article Title: A High-Efficacy CRISPR Interference System for Gene Function Discovery in Zymomonas mobilis Article Snippet: Oligonucleotides (2 μM each) were annealed in 1× CutSmart buffer (NEB) at 95°C for 5 min, followed by cooling to room temperature. .. For method two, fragments were amplified by PCR with primers oJMP197 and oJMP198 from a 78-nt oligonucleotide, followed by digestion with BsaI-HF-v2 (catalog number R3733; NEB) and purification with the Article Title: Bacterial defenses against a natural antibiotic promote collateral resilience to clinical antibiotics Article Snippet: Briefly, for unmarked deletions, approximately 1-kb fragments immediately upstream and downstream of the target locus were cloned using Gibson assembly into the pMQ30 suicide vector [ , ]. .. Fragments amplified from P . aeruginosa PA14 genomic DNA (gDNA) and cleaned up using the Purification:Article Title: Bacterial defenses against a natural antibiotic promote collateral resilience to clinical antibiotics Article Snippet: Briefly, for unmarked deletions, ∼1kb fragments immediately upstream and downstream of the target locus were cloned using Gibson assembly into the pMQ30 suicide vector , . .. Fragments amplified from P. aeruginosa PA14 genomic DNA (gDNA) and cleaned up using the Article Title: A High-Efficacy CRISPR Interference System for Gene Function Discovery in Zymomonas mobilis Article Snippet: Oligonucleotides (2 μM each) were annealed in 1× CutSmart buffer (NEB) at 95°C for 5 min, followed by cooling to room temperature. .. For method two, fragments were amplified by PCR with primers oJMP197 and oJMP198 from a 78-nt oligonucleotide, followed by digestion with BsaI-HF-v2 (catalog number R3733; NEB) and purification with the Article Title: Bacterial defenses against a natural antibiotic promote collateral resilience to clinical antibiotics Article Snippet: Briefly, for unmarked deletions, approximately 1-kb fragments immediately upstream and downstream of the target locus were cloned using Gibson assembly into the pMQ30 suicide vector [ , ]. .. Fragments amplified from P . aeruginosa PA14 genomic DNA (gDNA) and cleaned up using the Infection:Article Title: MicroRNA-21 depletion by CRISPR/Cas9 in CNE2 nasopharyngeal cells hinders proliferation and induces apoptosis by targeting the PI3K/AKT/MOTOR signaling pathway Article Snippet: The oligonucleotide sequence was synthesized by Shanghai Genechem Co., LTD., and each pair of oligonucleotides was transformed into DS-DNA fragments through annealing and then linked onto the Cas9-Easy-lentivirus vector. .. 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Genomic DNA from the bgp mutant strain was isolated by standard Isolation:Article Title: The Borrelia burgdorferi Glycosaminoglycan Binding Protein Bgp in the B31 Strain Is Not Essential for Infectivity despite Facilitating Adherence and Tissue Colonization Article Snippet: .. Genomic DNA from the bgp mutant strain was isolated by standard Article Title: Rapid Identification of Methylase Specificity (RIMS-seq) jointly identifies methylated motifs and generates shotgun sequencing of bacterial genomes Article Snippet: E. coli K12 MG1655 genomic DNA was extracted from a cell culture using the DNEasy Blood and Tissue kit (69504, Qiagen). .. All the other gDNA from the bacteria presented in were isolated using the DNA Purification:Article Title: The Borrelia burgdorferi Glycosaminoglycan Binding Protein Bgp in the B31 Strain Is Not Essential for Infectivity despite Facilitating Adherence and Tissue Colonization Article Snippet: .. Genomic DNA from the bgp mutant strain was isolated by standard Article Title: A High-Efficacy CRISPR Interference System for Gene Function Discovery in Zymomonas mobilis Article Snippet: Oligonucleotides (2 μM each) were annealed in 1× CutSmart buffer (NEB) at 95°C for 5 min, followed by cooling to room temperature. .. For method two, fragments were amplified by PCR with primers oJMP197 and oJMP198 from a 78-nt oligonucleotide, followed by digestion with BsaI-HF-v2 (catalog number R3733; NEB) and purification with the Article Title: A New Protocol for Targeted insertion using CRISPR-Cas9, Oligo Single-Stranded DNA and Protoplast Regeneration Article Snippet: .. Whole genome sequencing for off-targeted insertion analysis Leaves of N. benthamiana protoplast regenerants were collected for Article Title: Rapid Identification of Methylase Specificity (RIMS-seq) jointly identifies methylated motifs and generates shotgun sequencing of bacterial genomes Article Snippet: E. coli K12 MG1655 genomic DNA was extracted from a cell culture using the DNEasy Blood and Tissue kit (69504, Qiagen). .. All the other gDNA from the bacteria presented in were isolated using the Clone Assay:Article Title: The Borrelia burgdorferi Glycosaminoglycan Binding Protein Bgp in the B31 Strain Is Not Essential for Infectivity despite Facilitating Adherence and Tissue Colonization Article Snippet: .. Genomic DNA from the bgp mutant strain was isolated by standard Sequencing:Article Title: A New Protocol for Targeted insertion using CRISPR-Cas9, Oligo Single-Stranded DNA and Protoplast Regeneration Article Snippet: .. Whole genome sequencing for off-targeted insertion analysis Leaves of N. benthamiana protoplast regenerants were collected for |
![PYO induces expression of specific efflux systems, conferring cross-tolerance to fluoroquinolones. (A) Structures of PYO, 2 representative fluoroquinolones (CIP and LVX) and 2 representative aminoglycosides (GEN and TOB). PYO and fluoroquinolones are pumped by MexEF-OprN and MexGHI-OpmD, while aminoglycosides are not [ 21 , 22 ]. Rings with an aromatic character are highlighted in red. (B) Normalized cDNA levels for genes within operons coding for the 11 main RND efflux systems in P . <t>aeruginosa</t> (left; n = 3) and PYO-dose-dependent changes in expression of mexEF-oprN and mexGHI-opmD systems (right; n = 3). For full <t>qRT-PCR</t> dataset, see S1 – S3 Figs. (C) Effect of PYO on tolerance to CIP (1 μg/mL), LVX (1 μg/mL), and CST (16 μg/mL) in GMM ( n = 4). (D) Effect of PYO on tolerance to CIP (1 μg/mL) and TOB (40 μg/mL) in SCFM ( n = 4). PYO itself was not toxic under the experimental conditions [ 16 ] ( S4C Fig ). WT made 50–80 μM PYO as measured by absorbance of the culture supernatant at 691 nm. See S5A Fig for experimental design. (E) Effect on tolerance to CIP (1 μg/mL) in GMM caused by the presence of the 4 main phenazines produced by P . aeruginosa (PYO, PCA, PCN, and 1-OH-PHZ) ( n = 4). For this experiment, a Δ phz * strain that cannot produce or modify any phenazine was used (see Methods ). (F, G) Effect of PYO on lag during outgrowth after exposure to CIP in GMM. A representative field of view over different time points (F; magenta = WT::mApple, green = Δ phz ::GFP; see S1 Movie ) is shown together with the quantification of growth area on the agarose pads at time 0 hour and 15 hours (G). For these experiments, a culture of each strain tested was grown and exposed to CIP (10 μg/mL) separately, then cells of both cultures were washed, mixed, and placed together on a pad and imaged during outgrowth. The pads did not contain any PYO or CIP (see Methods and S5D Fig for details). White arrows in the displayed images point to regions with faster recovery of WT growth. The field of view displayed is marked with a black arrow in the quantification plot. The results for the experiment with swapped fluorescent proteins are shown in S4E Fig . See S4C Fig for complementary data about effects of PYO on lag. Scale bar: 20 μm. (H) Tolerance of Δ phz to CIP (1 μg/mL) in GMM in the presence of different concentrations of PYO ( n = 4). (G) Tolerance of Δ phz to CIP (1 μg/mL) in GMM upon artificial induction of the mexGHI-opmD operon with arabinose ( n = 4). The dashed green line marks the average survival of PYO-producing WT under similar conditions (without arabinose). Statistics: C, D, E, H—1-way ANOVA with Tukey HSD multiple comparison test, with asterisks showing significant differences relative to untreated Δ phz (no PYO); G, I—Welch unpaired t test (* p](https://storage.googleapis.com/bioz_article_images/PMC7946323/pbio.3001093.g002.jpg)
