monarch genomic dna purification kit  (New England Biolabs)


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    Structured Review

    New England Biolabs monarch genomic dna purification kit
    CasKAS can be performed on pre-sheared <t>DNA.</t> CasKAS was performed in vitro using the EMX1 sgRNA, first, conventionally, by carrying out the CasKAS reaction, then isolating and shearing genomic DNA, and also by pre-shearing the DNA and carrying out the CasKAS reaction on the fragmented DNA. The concern in that case is that the presence of sticky ends containing Gs and unprotected from the action of the N 3 -kethoxal would lower the background. This problem can be addressed by carrying out end repair on the sheared DNA prior to the CasKAS reaction.
    Monarch Genomic Dna Purification Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monarch genomic dna purification kit/product/New England Biolabs
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monarch genomic dna purification kit - by Bioz Stars, 2022-05
    97/100 stars

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    1) Product Images from "Direct profiling of genome-wide dCas9 and Cas9 specificity using ssDNA mapping (CasKAS)"

    Article Title: Direct profiling of genome-wide dCas9 and Cas9 specificity using ssDNA mapping (CasKAS)

    Journal: bioRxiv

    doi: 10.1101/2021.04.16.440202

    CasKAS can be performed on pre-sheared DNA. CasKAS was performed in vitro using the EMX1 sgRNA, first, conventionally, by carrying out the CasKAS reaction, then isolating and shearing genomic DNA, and also by pre-shearing the DNA and carrying out the CasKAS reaction on the fragmented DNA. The concern in that case is that the presence of sticky ends containing Gs and unprotected from the action of the N 3 -kethoxal would lower the background. This problem can be addressed by carrying out end repair on the sheared DNA prior to the CasKAS reaction.
    Figure Legend Snippet: CasKAS can be performed on pre-sheared DNA. CasKAS was performed in vitro using the EMX1 sgRNA, first, conventionally, by carrying out the CasKAS reaction, then isolating and shearing genomic DNA, and also by pre-shearing the DNA and carrying out the CasKAS reaction on the fragmented DNA. The concern in that case is that the presence of sticky ends containing Gs and unprotected from the action of the N 3 -kethoxal would lower the background. This problem can be addressed by carrying out end repair on the sheared DNA prior to the CasKAS reaction.

    Techniques Used: In Vitro

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    New England Biolabs monarch genomic dna purification kit
    CasKAS can be performed on pre-sheared <t>DNA.</t> CasKAS was performed in vitro using the EMX1 sgRNA, first, conventionally, by carrying out the CasKAS reaction, then isolating and shearing genomic DNA, and also by pre-shearing the DNA and carrying out the CasKAS reaction on the fragmented DNA. The concern in that case is that the presence of sticky ends containing Gs and unprotected from the action of the N 3 -kethoxal would lower the background. This problem can be addressed by carrying out end repair on the sheared DNA prior to the CasKAS reaction.
    Monarch Genomic Dna Purification Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monarch genomic dna purification kit/product/New England Biolabs
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monarch genomic dna purification kit - by Bioz Stars, 2022-05
    97/100 stars
      Buy from Supplier

    86
    New England Biolabs monarch gdna purification columns
    CasKAS can be performed on pre-sheared <t>DNA.</t> CasKAS was performed in vitro using the EMX1 sgRNA, first, conventionally, by carrying out the CasKAS reaction, then isolating and shearing genomic DNA, and also by pre-shearing the DNA and carrying out the CasKAS reaction on the fragmented DNA. The concern in that case is that the presence of sticky ends containing Gs and unprotected from the action of the N 3 -kethoxal would lower the background. This problem can be addressed by carrying out end repair on the sheared DNA prior to the CasKAS reaction.
    Monarch Gdna Purification Columns, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monarch gdna purification columns/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monarch gdna purification columns - by Bioz Stars, 2022-05
    86/100 stars
      Buy from Supplier

    86
    New England Biolabs monarch gdna purification kit
    CasKAS can be performed on pre-sheared <t>DNA.</t> CasKAS was performed in vitro using the EMX1 sgRNA, first, conventionally, by carrying out the CasKAS reaction, then isolating and shearing genomic DNA, and also by pre-shearing the DNA and carrying out the CasKAS reaction on the fragmented DNA. The concern in that case is that the presence of sticky ends containing Gs and unprotected from the action of the N 3 -kethoxal would lower the background. This problem can be addressed by carrying out end repair on the sheared DNA prior to the CasKAS reaction.
    Monarch Gdna Purification Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monarch gdna purification kit/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monarch gdna purification kit - by Bioz Stars, 2022-05
    86/100 stars
      Buy from Supplier

    Image Search Results


    CasKAS can be performed on pre-sheared DNA. CasKAS was performed in vitro using the EMX1 sgRNA, first, conventionally, by carrying out the CasKAS reaction, then isolating and shearing genomic DNA, and also by pre-shearing the DNA and carrying out the CasKAS reaction on the fragmented DNA. The concern in that case is that the presence of sticky ends containing Gs and unprotected from the action of the N 3 -kethoxal would lower the background. This problem can be addressed by carrying out end repair on the sheared DNA prior to the CasKAS reaction.

    Journal: bioRxiv

    Article Title: Direct profiling of genome-wide dCas9 and Cas9 specificity using ssDNA mapping (CasKAS)

    doi: 10.1101/2021.04.16.440202

    Figure Lengend Snippet: CasKAS can be performed on pre-sheared DNA. CasKAS was performed in vitro using the EMX1 sgRNA, first, conventionally, by carrying out the CasKAS reaction, then isolating and shearing genomic DNA, and also by pre-shearing the DNA and carrying out the CasKAS reaction on the fragmented DNA. The concern in that case is that the presence of sticky ends containing Gs and unprotected from the action of the N 3 -kethoxal would lower the background. This problem can be addressed by carrying out end repair on the sheared DNA prior to the CasKAS reaction.

    Article Snippet: Cells were then pelleted by centrifuging at 500 g for 5 minutes at 4 ° C. Genomic DNA was then extracted using the Monarch gDNA Purification Kit (NEB T3010S) following the standard protocol but with elution using 175 µ L 25 mM K3 BO3 at pH 7.0.

    Techniques: In Vitro