Monarch Genomic Dna Purification Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Direct profiling of genome-wide dCas9 and Cas9 specificity using ssDNA mapping (CasKAS)"
Article Title: Direct profiling of genome-wide dCas9 and Cas9 specificity using ssDNA mapping (CasKAS)
Figure Legend Snippet: CasKAS can be performed on pre-sheared DNA. CasKAS was performed in vitro using the EMX1 sgRNA, first, conventionally, by carrying out the CasKAS reaction, then isolating and shearing genomic DNA, and also by pre-shearing the DNA and carrying out the CasKAS reaction on the fragmented DNA. The concern in that case is that the presence of sticky ends containing Gs and unprotected from the action of the N 3 -kethoxal would lower the background. This problem can be addressed by carrying out end repair on the sheared DNA prior to the CasKAS reaction.
Techniques Used: In Vitro