dnasei  (New England Biolabs)


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    Name:
    Monarch Total RNA Miniprep Kit
    Description:
    Monarch Total RNA Miniprep Kit
    Catalog Number:
    T2010S
    Price:
    255
    Category:
    RNA Purification Kit Components
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    New England Biolabs dnasei
    Monarch Total RNA Miniprep Kit
    Monarch Total RNA Miniprep Kit
    https://www.bioz.com/result/dnasei/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dnasei - by Bioz Stars, 2021-07
    99/100 stars

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    Purification:

    Article Title: Dual Regulation of Phosphatidylserine Decarboxylase Expression by Envelope Stress Responses
    Article Snippet: Scanning and quantification were performed on a Li-Cor Odyssey-Fc imaging system, reading at 700 nm (for IscS detection) or 800 nm (for Flag detection). .. Reverse Transcriptase – PCRRNAs were purified using the NEB Monarch Total RNA MiniPrep Kit and further digested with Dnase I followed by clean up with the Qiagen Rneasy Mini kit. .. Reverse transcription was performed using Invitrogen SuperScript III First-Stand Synthesis System with random hexamers.

    Expressing:

    Article Title: Allele-specific expression changes dynamically during T cell activation in HLA and other autoimmune loci
    Article Snippet: .. For analysis of HLA-DQB1 expression on HH WT (C/C) and ALT (G/G) clones, RNA was extracted from clones using a Monarch Total RNA extraction kit (NEB). cDNA was synthesized using MaximaH RT (NEB) enzyme following manufacturer’s protocol and oligoDT primers. cDNA was diluted 1 in 4 with HLA-DQB1 (Assay ID:Hs00409790) and actinB (Assay ID:Hs01060665) probes and Taqman MasterMix (Thermofisher). .. Samples were run on an ARIAmx qPCR machine (Agilent) and data analyzed with Aria 1.5 (Agilent) software.

    Clone Assay:

    Article Title: Allele-specific expression changes dynamically during T cell activation in HLA and other autoimmune loci
    Article Snippet: .. For analysis of HLA-DQB1 expression on HH WT (C/C) and ALT (G/G) clones, RNA was extracted from clones using a Monarch Total RNA extraction kit (NEB). cDNA was synthesized using MaximaH RT (NEB) enzyme following manufacturer’s protocol and oligoDT primers. cDNA was diluted 1 in 4 with HLA-DQB1 (Assay ID:Hs00409790) and actinB (Assay ID:Hs01060665) probes and Taqman MasterMix (Thermofisher). .. Samples were run on an ARIAmx qPCR machine (Agilent) and data analyzed with Aria 1.5 (Agilent) software.

    RNA Extraction:

    Article Title: Allele-specific expression changes dynamically during T cell activation in HLA and other autoimmune loci
    Article Snippet: .. For analysis of HLA-DQB1 expression on HH WT (C/C) and ALT (G/G) clones, RNA was extracted from clones using a Monarch Total RNA extraction kit (NEB). cDNA was synthesized using MaximaH RT (NEB) enzyme following manufacturer’s protocol and oligoDT primers. cDNA was diluted 1 in 4 with HLA-DQB1 (Assay ID:Hs00409790) and actinB (Assay ID:Hs01060665) probes and Taqman MasterMix (Thermofisher). .. Samples were run on an ARIAmx qPCR machine (Agilent) and data analyzed with Aria 1.5 (Agilent) software.

    Article Title: The plant mobile domain proteins MAIN and MAIL1 interact with the phosphatase PP7L to regulate gene expression and silence transposable elements in Arabidopsis thaliana
    Article Snippet: Western blots were developed using Substrat HRP Immobilon Western (Merck Millipore, WBKLS0500). .. RNA extraction Total RNA was extracted from aerial parts of 3-week-old seedlings grown on soil using either RNeasy Plant Mini Kit (Qiagen, 74904) or Monarch Total RNA Miniprep Kit (NEB, T2010) according to the manufacturer’s protocols. .. RNA sequencing RNA-seq libraries were generated from 1μg of input RNA using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7490) according to the manufacturer’s protocols.

    Synthesized:

    Article Title: Allele-specific expression changes dynamically during T cell activation in HLA and other autoimmune loci
    Article Snippet: .. For analysis of HLA-DQB1 expression on HH WT (C/C) and ALT (G/G) clones, RNA was extracted from clones using a Monarch Total RNA extraction kit (NEB). cDNA was synthesized using MaximaH RT (NEB) enzyme following manufacturer’s protocol and oligoDT primers. cDNA was diluted 1 in 4 with HLA-DQB1 (Assay ID:Hs00409790) and actinB (Assay ID:Hs01060665) probes and Taqman MasterMix (Thermofisher). .. Samples were run on an ARIAmx qPCR machine (Agilent) and data analyzed with Aria 1.5 (Agilent) software.

    Polymerase Chain Reaction:

    Article Title: High lncRNA MEG3 expression is associated with high mortality rates in patients with sepsis and increased lipopolysaccharide-induced renal epithelial cell and cardiomyocyte apoptosis
    Article Snippet: Cells were cultivated in DMEM medium (Sigma-Aldrich; Merck KGaA) containing 10% FBS (Sigma-Aldrich; Merck KGaA) at 37°C with 5% CO2 . .. Reverse transcription-quantitative PCR (RT-qPCR) Total RNA was extracted from plamsa, A16 cardiomyocytes and renal mixed epithelial cells using Monarch® Total RNA Miniprep kit (New England BioLabs, Inc.) according to manufacturer's protocol, followed by reverse transcription using SuperScript™ III Reverse Transcriptase (Thermo Fisher Scientific., lnc.) and preparation of qPCR reactions using SYBR® Green Quantitative RT-qPCR kit (Sigma-Aldrich; Merck KGaA), according to the manufacturers' protocols. .. Primers of lncRNA MEG3 and β-actin endogous control were designed and synthesized by Shanghai GenePharma Co., Ltd.

    Quantitative RT-PCR:

    Article Title: High lncRNA MEG3 expression is associated with high mortality rates in patients with sepsis and increased lipopolysaccharide-induced renal epithelial cell and cardiomyocyte apoptosis
    Article Snippet: Cells were cultivated in DMEM medium (Sigma-Aldrich; Merck KGaA) containing 10% FBS (Sigma-Aldrich; Merck KGaA) at 37°C with 5% CO2 . .. Reverse transcription-quantitative PCR (RT-qPCR) Total RNA was extracted from plamsa, A16 cardiomyocytes and renal mixed epithelial cells using Monarch® Total RNA Miniprep kit (New England BioLabs, Inc.) according to manufacturer's protocol, followed by reverse transcription using SuperScript™ III Reverse Transcriptase (Thermo Fisher Scientific., lnc.) and preparation of qPCR reactions using SYBR® Green Quantitative RT-qPCR kit (Sigma-Aldrich; Merck KGaA), according to the manufacturers' protocols. .. Primers of lncRNA MEG3 and β-actin endogous control were designed and synthesized by Shanghai GenePharma Co., Ltd.

    Real-time Polymerase Chain Reaction:

    Article Title: High lncRNA MEG3 expression is associated with high mortality rates in patients with sepsis and increased lipopolysaccharide-induced renal epithelial cell and cardiomyocyte apoptosis
    Article Snippet: Cells were cultivated in DMEM medium (Sigma-Aldrich; Merck KGaA) containing 10% FBS (Sigma-Aldrich; Merck KGaA) at 37°C with 5% CO2 . .. Reverse transcription-quantitative PCR (RT-qPCR) Total RNA was extracted from plamsa, A16 cardiomyocytes and renal mixed epithelial cells using Monarch® Total RNA Miniprep kit (New England BioLabs, Inc.) according to manufacturer's protocol, followed by reverse transcription using SuperScript™ III Reverse Transcriptase (Thermo Fisher Scientific., lnc.) and preparation of qPCR reactions using SYBR® Green Quantitative RT-qPCR kit (Sigma-Aldrich; Merck KGaA), according to the manufacturers' protocols. .. Primers of lncRNA MEG3 and β-actin endogous control were designed and synthesized by Shanghai GenePharma Co., Ltd.

    SYBR Green Assay:

    Article Title: High lncRNA MEG3 expression is associated with high mortality rates in patients with sepsis and increased lipopolysaccharide-induced renal epithelial cell and cardiomyocyte apoptosis
    Article Snippet: Cells were cultivated in DMEM medium (Sigma-Aldrich; Merck KGaA) containing 10% FBS (Sigma-Aldrich; Merck KGaA) at 37°C with 5% CO2 . .. Reverse transcription-quantitative PCR (RT-qPCR) Total RNA was extracted from plamsa, A16 cardiomyocytes and renal mixed epithelial cells using Monarch® Total RNA Miniprep kit (New England BioLabs, Inc.) according to manufacturer's protocol, followed by reverse transcription using SuperScript™ III Reverse Transcriptase (Thermo Fisher Scientific., lnc.) and preparation of qPCR reactions using SYBR® Green Quantitative RT-qPCR kit (Sigma-Aldrich; Merck KGaA), according to the manufacturers' protocols. .. Primers of lncRNA MEG3 and β-actin endogous control were designed and synthesized by Shanghai GenePharma Co., Ltd.

    Modification:

    Article Title: Early blood transcriptomic markers of necrotizing enterocolitis in preterm pigs
    Article Snippet: Total blood RNA from the 1st storage method was extracted using MagMax 96 blood RNA isolation kit according to the manufacturer’s instructions. .. Total blood RNA from DBS was extracted using Monarch Total RNA Miniprep Kit (New England Biolabs, USA) with a modified protocol. .. Briefly, one full DBS was cut into small pieces and incubated in 600 µl 1X DNA/RNA Protection Reagent at room temperature.

    RNA Sequencing Assay:

    Article Title: Direct Metatranscriptome RNA-seq and Multiplex RT-PCR Amplicon Sequencing on Nanopore MinION – Promising Strategies for Multiplex Identification of Viable Pathogens in Food
    Article Snippet: Cocktail culture inactivated with 13.4 mmol/L of sodium hypochlorite was used as the negative control to test whether metatranscriptome sequencing can eliminate false positive identification. .. Direct Metatranscriptome RNA-seq on NGS iSeq 100 Sequencing System for Control RNA extracts of 24-h cocktail culture of E. coli O157:H7, S. enteritidis , and L. monocytogenes in BHI or LJE was obtained by NEB Monarch Total RNA Miniprep Kit including DNase I as we did for direct metatranscriptome RNA-seq on a MinION sequencer (Oxford Nanopore). .. The quantification and quality control of samples were tested by Qubit RNA HS Assay (Thermo Fisher Scientific) and 2100 Bioanalyzer system (RNA 6000 Pico Kit/High Sensitivity DNA Kit and 2100 Expert Software, Agilent, Santa Clara, CA, United States).

    Next-Generation Sequencing:

    Article Title: Direct Metatranscriptome RNA-seq and Multiplex RT-PCR Amplicon Sequencing on Nanopore MinION – Promising Strategies for Multiplex Identification of Viable Pathogens in Food
    Article Snippet: Cocktail culture inactivated with 13.4 mmol/L of sodium hypochlorite was used as the negative control to test whether metatranscriptome sequencing can eliminate false positive identification. .. Direct Metatranscriptome RNA-seq on NGS iSeq 100 Sequencing System for Control RNA extracts of 24-h cocktail culture of E. coli O157:H7, S. enteritidis , and L. monocytogenes in BHI or LJE was obtained by NEB Monarch Total RNA Miniprep Kit including DNase I as we did for direct metatranscriptome RNA-seq on a MinION sequencer (Oxford Nanopore). .. The quantification and quality control of samples were tested by Qubit RNA HS Assay (Thermo Fisher Scientific) and 2100 Bioanalyzer system (RNA 6000 Pico Kit/High Sensitivity DNA Kit and 2100 Expert Software, Agilent, Santa Clara, CA, United States).

    Sequencing:

    Article Title: Direct Metatranscriptome RNA-seq and Multiplex RT-PCR Amplicon Sequencing on Nanopore MinION – Promising Strategies for Multiplex Identification of Viable Pathogens in Food
    Article Snippet: Cocktail culture inactivated with 13.4 mmol/L of sodium hypochlorite was used as the negative control to test whether metatranscriptome sequencing can eliminate false positive identification. .. Direct Metatranscriptome RNA-seq on NGS iSeq 100 Sequencing System for Control RNA extracts of 24-h cocktail culture of E. coli O157:H7, S. enteritidis , and L. monocytogenes in BHI or LJE was obtained by NEB Monarch Total RNA Miniprep Kit including DNase I as we did for direct metatranscriptome RNA-seq on a MinION sequencer (Oxford Nanopore). .. The quantification and quality control of samples were tested by Qubit RNA HS Assay (Thermo Fisher Scientific) and 2100 Bioanalyzer system (RNA 6000 Pico Kit/High Sensitivity DNA Kit and 2100 Expert Software, Agilent, Santa Clara, CA, United States).

    Cell Culture:

    Article Title: Finding Nemo’s clock reveals switch from nocturnal to diurnal activity
    Article Snippet: The pellet was washed twice with 1× PBS and stored at −80 °C until RNA extraction. .. Total RNA was extracted using Monarch Total RNA Miniprep Kit (New England Biolabs, Frankfurt am Main, Germany) following the manufacturer’s instructions for cultured mammalian cells and tissue including DNase treatment. .. Deviating from that protocol, whole animals were homogenized in 1× DNA/RNA Protection Reagent using a bead beater (MagNa Lyser, Roche Diagnostics, Mannheim, Germany) with glass beads (diameter 1.25–1.65 mm, Carl Roth, Karlsruhe, Germany) at 5000 rpm for up to 6 min.

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    New England Biolabs monarch total rna extraction kit
    Scheme depicting HLA allelic expression quantification with HLA-personalized genome. In order to quantify robustly allele-specific expression in the highly polymorphic HLA genes, we first create an HLA-personalized genome per individual. We do this by inserting into the reference genome the <t>cDNA</t> sequences of each HLA allele as separate sequences (12 in total giv en that we sequenced or typed 6 HLA genes), and masking the exonic sequences corresponding to those cDNAs in chromosome 6 of the reference genome. Next, we map the <t>RNA-seq</t> reads to this HLA-personalized genome, we remove PCR duplicates and we count the number of uniquely mapped reads to each HLA cDNA allele.
    Monarch Total Rna Extraction Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monarch total rna extraction kit/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monarch total rna extraction kit - by Bioz Stars, 2021-07
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    Scheme depicting HLA allelic expression quantification with HLA-personalized genome. In order to quantify robustly allele-specific expression in the highly polymorphic HLA genes, we first create an HLA-personalized genome per individual. We do this by inserting into the reference genome the cDNA sequences of each HLA allele as separate sequences (12 in total giv en that we sequenced or typed 6 HLA genes), and masking the exonic sequences corresponding to those cDNAs in chromosome 6 of the reference genome. Next, we map the RNA-seq reads to this HLA-personalized genome, we remove PCR duplicates and we count the number of uniquely mapped reads to each HLA cDNA allele.

    Journal: Nature genetics

    Article Title: Allele-specific expression changes dynamically during T cell activation in HLA and other autoimmune loci

    doi: 10.1038/s41588-020-0579-4

    Figure Lengend Snippet: Scheme depicting HLA allelic expression quantification with HLA-personalized genome. In order to quantify robustly allele-specific expression in the highly polymorphic HLA genes, we first create an HLA-personalized genome per individual. We do this by inserting into the reference genome the cDNA sequences of each HLA allele as separate sequences (12 in total giv en that we sequenced or typed 6 HLA genes), and masking the exonic sequences corresponding to those cDNAs in chromosome 6 of the reference genome. Next, we map the RNA-seq reads to this HLA-personalized genome, we remove PCR duplicates and we count the number of uniquely mapped reads to each HLA cDNA allele.

    Article Snippet: For analysis of HLA-DQB1 expression on HH WT (C/C) and ALT (G/G) clones, RNA was extracted from clones using a Monarch Total RNA extraction kit (NEB). cDNA was synthesized using MaximaH RT (NEB) enzyme following manufacturer’s protocol and oligoDT primers. cDNA was diluted 1 in 4 with HLA-DQB1 (Assay ID:Hs00409790) and actinB (Assay ID:Hs01060665) probes and Taqman MasterMix (Thermofisher).

    Techniques: Expressing, RNA Sequencing Assay, Polymerase Chain Reaction