rna  (New England Biolabs)


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    Name:
    Monarch Total RNA Miniprep Kit
    Description:
    Monarch Total RNA Miniprep Kit
    Catalog Number:
    T2010S
    Price:
    255
    Category:
    RNA Purification Kit Components
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    Structured Review

    New England Biolabs rna
    Monarch Total RNA Miniprep Kit
    Monarch Total RNA Miniprep Kit
    https://www.bioz.com/result/rna/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rna - by Bioz Stars, 2021-05
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: High lncRNA MEG3 expression is associated with high mortality rates in patients with sepsis and increased lipopolysaccharide-induced renal epithelial cell and cardiomyocyte apoptosis
    Article Snippet: Cells were cultivated in DMEM medium (Sigma-Aldrich; Merck KGaA) containing 10% FBS (Sigma-Aldrich; Merck KGaA) at 37°C with 5% CO2 . .. Reverse transcription-quantitative PCR (RT-qPCR) Total RNA was extracted from plamsa, A16 cardiomyocytes and renal mixed epithelial cells using Monarch® Total RNA Miniprep kit (New England BioLabs, Inc.) according to manufacturer's protocol, followed by reverse transcription using SuperScript™ III Reverse Transcriptase (Thermo Fisher Scientific., lnc.) and preparation of qPCR reactions using SYBR® Green Quantitative RT-qPCR kit (Sigma-Aldrich; Merck KGaA), according to the manufacturers' protocols. .. Primers of lncRNA MEG3 and β-actin endogous control were designed and synthesized by Shanghai GenePharma Co., Ltd.

    Article Title: Raman spectroscopy links differentiating osteoblast matrix signatures to pro-angiogenic potential
    Article Snippet: A VEGF-A mouse sandwich ELISA kit and reagents (R & D Systems, USA) were used to quantify natural and recombinant VEGF (VEGF120 and VEGF164) in the collected conditioned media following the manufacturers instruction. .. Reverse transcription-quantitative PCR (RT-qPCR) RNA from the cultured OBs were isolated from each preparation using the Monarch® Total RNA Miniprep Kit (New England Biolabs, UK) following the manufacturers instruction. .. Five hundred ng of RNA per condition was reverse transcribed using the LunaScript® RT Supermix (New England Biolabs, UK) as per the manufacturer's recommendation.

    Quantitative RT-PCR:

    Article Title: High lncRNA MEG3 expression is associated with high mortality rates in patients with sepsis and increased lipopolysaccharide-induced renal epithelial cell and cardiomyocyte apoptosis
    Article Snippet: Cells were cultivated in DMEM medium (Sigma-Aldrich; Merck KGaA) containing 10% FBS (Sigma-Aldrich; Merck KGaA) at 37°C with 5% CO2 . .. Reverse transcription-quantitative PCR (RT-qPCR) Total RNA was extracted from plamsa, A16 cardiomyocytes and renal mixed epithelial cells using Monarch® Total RNA Miniprep kit (New England BioLabs, Inc.) according to manufacturer's protocol, followed by reverse transcription using SuperScript™ III Reverse Transcriptase (Thermo Fisher Scientific., lnc.) and preparation of qPCR reactions using SYBR® Green Quantitative RT-qPCR kit (Sigma-Aldrich; Merck KGaA), according to the manufacturers' protocols. .. Primers of lncRNA MEG3 and β-actin endogous control were designed and synthesized by Shanghai GenePharma Co., Ltd.

    Article Title: Raman spectroscopy links differentiating osteoblast matrix signatures to pro-angiogenic potential
    Article Snippet: A VEGF-A mouse sandwich ELISA kit and reagents (R & D Systems, USA) were used to quantify natural and recombinant VEGF (VEGF120 and VEGF164) in the collected conditioned media following the manufacturers instruction. .. Reverse transcription-quantitative PCR (RT-qPCR) RNA from the cultured OBs were isolated from each preparation using the Monarch® Total RNA Miniprep Kit (New England Biolabs, UK) following the manufacturers instruction. .. Five hundred ng of RNA per condition was reverse transcribed using the LunaScript® RT Supermix (New England Biolabs, UK) as per the manufacturer's recommendation.

    Real-time Polymerase Chain Reaction:

    Article Title: High lncRNA MEG3 expression is associated with high mortality rates in patients with sepsis and increased lipopolysaccharide-induced renal epithelial cell and cardiomyocyte apoptosis
    Article Snippet: Cells were cultivated in DMEM medium (Sigma-Aldrich; Merck KGaA) containing 10% FBS (Sigma-Aldrich; Merck KGaA) at 37°C with 5% CO2 . .. Reverse transcription-quantitative PCR (RT-qPCR) Total RNA was extracted from plamsa, A16 cardiomyocytes and renal mixed epithelial cells using Monarch® Total RNA Miniprep kit (New England BioLabs, Inc.) according to manufacturer's protocol, followed by reverse transcription using SuperScript™ III Reverse Transcriptase (Thermo Fisher Scientific., lnc.) and preparation of qPCR reactions using SYBR® Green Quantitative RT-qPCR kit (Sigma-Aldrich; Merck KGaA), according to the manufacturers' protocols. .. Primers of lncRNA MEG3 and β-actin endogous control were designed and synthesized by Shanghai GenePharma Co., Ltd.

    SYBR Green Assay:

    Article Title: High lncRNA MEG3 expression is associated with high mortality rates in patients with sepsis and increased lipopolysaccharide-induced renal epithelial cell and cardiomyocyte apoptosis
    Article Snippet: Cells were cultivated in DMEM medium (Sigma-Aldrich; Merck KGaA) containing 10% FBS (Sigma-Aldrich; Merck KGaA) at 37°C with 5% CO2 . .. Reverse transcription-quantitative PCR (RT-qPCR) Total RNA was extracted from plamsa, A16 cardiomyocytes and renal mixed epithelial cells using Monarch® Total RNA Miniprep kit (New England BioLabs, Inc.) according to manufacturer's protocol, followed by reverse transcription using SuperScript™ III Reverse Transcriptase (Thermo Fisher Scientific., lnc.) and preparation of qPCR reactions using SYBR® Green Quantitative RT-qPCR kit (Sigma-Aldrich; Merck KGaA), according to the manufacturers' protocols. .. Primers of lncRNA MEG3 and β-actin endogous control were designed and synthesized by Shanghai GenePharma Co., Ltd.

    Modification:

    Article Title: Early blood transcriptomic markers of necrotizing enterocolitis in preterm pigs
    Article Snippet: Total blood RNA from the 1st storage method was extracted using MagMax 96 blood RNA isolation kit according to the manufacturer’s instructions. .. Total blood RNA from DBS was extracted using Monarch Total RNA Miniprep Kit (New England Biolabs, USA) with a modified protocol. .. Briefly, one full DBS was cut into small pieces and incubated in 600 µl 1X DNA/RNA Protection Reagent at room temperature.

    RNA Extraction:

    Article Title: Slaying the last unicorn - discovery of histones in the microalga Nanochlorum eucaryotum
    Article Snippet: All cultures were sealed with parafilm to prevent moisture loss during incubation. .. Total RNA extraction Total RNA was isolated from a 31-day-old N. eucaryotum (SAG 55.87) culture consisting of ~1.9 × 107 cells in total. .. To minimize the risk of bacterial contamination, 0.2 μm-filtered lysozyme solution was added into the culture to a final concentration of 0.5 mg/mL two days prior to cell harvest.

    Article Title: The plant mobile domain proteins MAIN and MAIL1 interact with the phosphatase PP7L to regulate gene expression and silence transposable elements in Arabidopsis thaliana
    Article Snippet: Western blots were developed using Substrat HRP Immobilon Western (Merck Millipore, WBKLS0500). .. RNA extraction Total RNA was extracted from aerial parts of 3-week-old seedlings grown on soil using either RNeasy Plant Mini Kit (Qiagen, 74904) or Monarch Total RNA Miniprep Kit (NEB, T2010) according to the manufacturer’s protocols. .. RNA sequencing RNA-seq libraries were generated from 1μg of input RNA using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7490) according to the manufacturer’s protocols.

    Isolation:

    Article Title: Slaying the last unicorn - discovery of histones in the microalga Nanochlorum eucaryotum
    Article Snippet: All cultures were sealed with parafilm to prevent moisture loss during incubation. .. Total RNA extraction Total RNA was isolated from a 31-day-old N. eucaryotum (SAG 55.87) culture consisting of ~1.9 × 107 cells in total. .. To minimize the risk of bacterial contamination, 0.2 μm-filtered lysozyme solution was added into the culture to a final concentration of 0.5 mg/mL two days prior to cell harvest.

    Article Title: Raman spectroscopy links differentiating osteoblast matrix signatures to pro-angiogenic potential
    Article Snippet: A VEGF-A mouse sandwich ELISA kit and reagents (R & D Systems, USA) were used to quantify natural and recombinant VEGF (VEGF120 and VEGF164) in the collected conditioned media following the manufacturers instruction. .. Reverse transcription-quantitative PCR (RT-qPCR) RNA from the cultured OBs were isolated from each preparation using the Monarch® Total RNA Miniprep Kit (New England Biolabs, UK) following the manufacturers instruction. .. Five hundred ng of RNA per condition was reverse transcribed using the LunaScript® RT Supermix (New England Biolabs, UK) as per the manufacturer's recommendation.

    RNA Sequencing Assay:

    Article Title: Direct Metatranscriptome RNA-seq and Multiplex RT-PCR Amplicon Sequencing on Nanopore MinION – Promising Strategies for Multiplex Identification of Viable Pathogens in Food
    Article Snippet: Cocktail culture inactivated with 13.4 mmol/L of sodium hypochlorite was used as the negative control to test whether metatranscriptome sequencing can eliminate false positive identification. .. Direct Metatranscriptome RNA-seq on NGS iSeq 100 Sequencing System for Control RNA extracts of 24-h cocktail culture of E. coli O157:H7, S. enteritidis , and L. monocytogenes in BHI or LJE was obtained by NEB Monarch Total RNA Miniprep Kit including DNase I as we did for direct metatranscriptome RNA-seq on a MinION sequencer (Oxford Nanopore). .. The quantification and quality control of samples were tested by Qubit RNA HS Assay (Thermo Fisher Scientific) and 2100 Bioanalyzer system (RNA 6000 Pico Kit/High Sensitivity DNA Kit and 2100 Expert Software, Agilent, Santa Clara, CA, United States).

    Next-Generation Sequencing:

    Article Title: Direct Metatranscriptome RNA-seq and Multiplex RT-PCR Amplicon Sequencing on Nanopore MinION – Promising Strategies for Multiplex Identification of Viable Pathogens in Food
    Article Snippet: Cocktail culture inactivated with 13.4 mmol/L of sodium hypochlorite was used as the negative control to test whether metatranscriptome sequencing can eliminate false positive identification. .. Direct Metatranscriptome RNA-seq on NGS iSeq 100 Sequencing System for Control RNA extracts of 24-h cocktail culture of E. coli O157:H7, S. enteritidis , and L. monocytogenes in BHI or LJE was obtained by NEB Monarch Total RNA Miniprep Kit including DNase I as we did for direct metatranscriptome RNA-seq on a MinION sequencer (Oxford Nanopore). .. The quantification and quality control of samples were tested by Qubit RNA HS Assay (Thermo Fisher Scientific) and 2100 Bioanalyzer system (RNA 6000 Pico Kit/High Sensitivity DNA Kit and 2100 Expert Software, Agilent, Santa Clara, CA, United States).

    Sequencing:

    Article Title: Direct Metatranscriptome RNA-seq and Multiplex RT-PCR Amplicon Sequencing on Nanopore MinION – Promising Strategies for Multiplex Identification of Viable Pathogens in Food
    Article Snippet: Cocktail culture inactivated with 13.4 mmol/L of sodium hypochlorite was used as the negative control to test whether metatranscriptome sequencing can eliminate false positive identification. .. Direct Metatranscriptome RNA-seq on NGS iSeq 100 Sequencing System for Control RNA extracts of 24-h cocktail culture of E. coli O157:H7, S. enteritidis , and L. monocytogenes in BHI or LJE was obtained by NEB Monarch Total RNA Miniprep Kit including DNase I as we did for direct metatranscriptome RNA-seq on a MinION sequencer (Oxford Nanopore). .. The quantification and quality control of samples were tested by Qubit RNA HS Assay (Thermo Fisher Scientific) and 2100 Bioanalyzer system (RNA 6000 Pico Kit/High Sensitivity DNA Kit and 2100 Expert Software, Agilent, Santa Clara, CA, United States).

    Cell Culture:

    Article Title: Finding Nemo’s clock reveals switch from nocturnal to diurnal activity
    Article Snippet: The pellet was washed twice with 1× PBS and stored at −80 °C until RNA extraction. .. Total RNA was extracted using Monarch Total RNA Miniprep Kit (New England Biolabs, Frankfurt am Main, Germany) following the manufacturer’s instructions for cultured mammalian cells and tissue including DNase treatment. .. Deviating from that protocol, whole animals were homogenized in 1× DNA/RNA Protection Reagent using a bead beater (MagNa Lyser, Roche Diagnostics, Mannheim, Germany) with glass beads (diameter 1.25–1.65 mm, Carl Roth, Karlsruhe, Germany) at 5000 rpm for up to 6 min.

    Article Title: Raman spectroscopy links differentiating osteoblast matrix signatures to pro-angiogenic potential
    Article Snippet: A VEGF-A mouse sandwich ELISA kit and reagents (R & D Systems, USA) were used to quantify natural and recombinant VEGF (VEGF120 and VEGF164) in the collected conditioned media following the manufacturers instruction. .. Reverse transcription-quantitative PCR (RT-qPCR) RNA from the cultured OBs were isolated from each preparation using the Monarch® Total RNA Miniprep Kit (New England Biolabs, UK) following the manufacturers instruction. .. Five hundred ng of RNA per condition was reverse transcribed using the LunaScript® RT Supermix (New England Biolabs, UK) as per the manufacturer's recommendation.

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    New England Biolabs total rna extraction total rna
    Abundance of histone transcripts compared to the remainder of de novo-assembled transcripts in Nanochlorum <t>eucaryotum</t> (SAG 55.87) and five other eukaryotes, including three algae (C. reinhardtii, D. tertiolecta, C. zofingiensis). Abundance of the most highly expressed histone transcript (solid lines) and the summed abundance of all histone transcripts (dashed lines) is indicated for each species. See Methods for details on the underlying <t>RNA-Seq</t> data, assembly and quantification process.
    Total Rna Extraction Total Rna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/total rna extraction total rna/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    total rna extraction total rna - by Bioz Stars, 2021-05
    99/100 stars
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    Abundance of histone transcripts compared to the remainder of de novo-assembled transcripts in Nanochlorum eucaryotum (SAG 55.87) and five other eukaryotes, including three algae (C. reinhardtii, D. tertiolecta, C. zofingiensis). Abundance of the most highly expressed histone transcript (solid lines) and the summed abundance of all histone transcripts (dashed lines) is indicated for each species. See Methods for details on the underlying RNA-Seq data, assembly and quantification process.

    Journal: bioRxiv

    Article Title: Slaying the last unicorn - discovery of histones in the microalga Nanochlorum eucaryotum

    doi: 10.1101/2020.10.22.350256

    Figure Lengend Snippet: Abundance of histone transcripts compared to the remainder of de novo-assembled transcripts in Nanochlorum eucaryotum (SAG 55.87) and five other eukaryotes, including three algae (C. reinhardtii, D. tertiolecta, C. zofingiensis). Abundance of the most highly expressed histone transcript (solid lines) and the summed abundance of all histone transcripts (dashed lines) is indicated for each species. See Methods for details on the underlying RNA-Seq data, assembly and quantification process.

    Article Snippet: Total RNA extraction Total RNA was isolated from a 31-day-old N. eucaryotum (SAG 55.87) culture consisting of ~1.9 × 107 cells in total.

    Techniques: RNA Sequencing Assay