full length uhrf1 ha  (Sino Biological)


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  • 94
    Name:
    UHRF1 cDNA ORF Clone Mouse C HA tag
    Description:
    Full length Clone DNA of Mouse ubiquitin like containing PHD and RING finger domains 1 with C terminal HA tag
    Catalog Number:
    MG53591-CY
    Price:
    215.0
    Category:
    cDNA Clone
    Applications:
    Stable or Transient mammalian expression
    Size:
    1Unit
    Product Aliases:
    AL022808 cDNA ORF Clone Mouse, ICBP90 cDNA ORF Clone Mouse, Np95 cDNA ORF Clone Mouse, RNF106 cDNA ORF Clone Mouse
    Molecule Name:
    UHRF1,RNF106,
    Buy from Supplier


    Structured Review

    Sino Biological full length uhrf1 ha
    UHRF1 cDNA ORF Clone Mouse C HA tag
    Full length Clone DNA of Mouse ubiquitin like containing PHD and RING finger domains 1 with C terminal HA tag
    https://www.bioz.com/result/full length uhrf1 ha/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    full length uhrf1 ha - by Bioz Stars, 2021-07
    94/100 stars

    Images

    1) Product Images from "Setd1a Plays Pivotal Roles for the Survival and Proliferation of Retinal Progenitors via Histone Modifications of Uhrf1"

    Article Title: Setd1a Plays Pivotal Roles for the Survival and Proliferation of Retinal Progenitors via Histone Modifications of Uhrf1

    Journal: Investigative Ophthalmology & Visual Science

    doi: 10.1167/iovs.62.6.1

    Expression of Uhrf1 transcripts was decreased by the expression of shSetd1. ( A ) Schematic representation of alternative splicing products of Uhrf1 . Adapted from the schematic data of asia.ensembl.org. ( B ) . Transition of expression level of transcripts of Uhrf1 during mouse retinal development from RNA-Seq data of whole mouse retina (GSE87064). 32 ( C ) Retinas at E17 were transfected with control or shSetd1a plasmids with an EGFP expression vector and cultured 2 days. EGFP positive-cells were purified, and RNA-Seq was performed. Expression levels of Uhrf1 splicing variants in the control or shSet1a-expressing retinas are shown. Values are average of 3 or 4 samples of RNA-Seq (GSE154498) data with standard deviation. *q
    Figure Legend Snippet: Expression of Uhrf1 transcripts was decreased by the expression of shSetd1. ( A ) Schematic representation of alternative splicing products of Uhrf1 . Adapted from the schematic data of asia.ensembl.org. ( B ) . Transition of expression level of transcripts of Uhrf1 during mouse retinal development from RNA-Seq data of whole mouse retina (GSE87064). 32 ( C ) Retinas at E17 were transfected with control or shSetd1a plasmids with an EGFP expression vector and cultured 2 days. EGFP positive-cells were purified, and RNA-Seq was performed. Expression levels of Uhrf1 splicing variants in the control or shSet1a-expressing retinas are shown. Values are average of 3 or 4 samples of RNA-Seq (GSE154498) data with standard deviation. *q

    Techniques Used: Expressing, RNA Sequencing Assay, Transfection, Plasmid Preparation, Cell Culture, Purification, Standard Deviation

    Expression of shUhrf1 perturbed retinal development. Plasmids encoding control, shUhrf1, UHRF1, or shSetd1a and EGFP expression plasmids were electroporated into mouse retina at E14 (B, C) or E17 (A, D – F) . Combination of transfected plasmids are indicated in the figures. ( A ) After sorting EGFP-positive cells, total RNA was purified and served to RT-qPCR. The averages of three independent samples with standard deviations are shown. * p
    Figure Legend Snippet: Expression of shUhrf1 perturbed retinal development. Plasmids encoding control, shUhrf1, UHRF1, or shSetd1a and EGFP expression plasmids were electroporated into mouse retina at E14 (B, C) or E17 (A, D – F) . Combination of transfected plasmids are indicated in the figures. ( A ) After sorting EGFP-positive cells, total RNA was purified and served to RT-qPCR. The averages of three independent samples with standard deviations are shown. * p

    Techniques Used: Expressing, Transfection, Purification, Quantitative RT-PCR

    Related Articles

    Inverse PCR:

    Article Title: Setd1a Plays Pivotal Roles for the Survival and Proliferation of Retinal Progenitors via Histone Modifications of Uhrf1
    Article Snippet: Setd1a mutant lacking SET domain (from AA 1577 to 1700) was constructed by KOD‐Plus‐Mutagenesis Kit (Toyobo). .. The full-length Uhrf1_HA was purchased from Sino Biological (MG53591-CY, China), and shUhrf1‐resistant Uhrf1 was made by inverse PCR using the KOD‐Plus‐Mutagenesis Kit (Toyobo). .. All the cDNAs for over-expression were subcloned into pCAG-KS.

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  • 94
    Sino Biological full length uhrf1 ha
    Expression of <t>Uhrf1</t> transcripts was decreased by the expression of shSetd1. ( A ) Schematic representation of alternative splicing products of Uhrf1 . Adapted from the schematic data of asia.ensembl.org. ( B ) . Transition of expression level of transcripts of Uhrf1 during mouse retinal development from RNA-Seq data of whole mouse retina (GSE87064). 32 ( C ) Retinas at E17 were transfected with control or shSetd1a plasmids with an EGFP expression vector and cultured 2 days. EGFP positive-cells were purified, and RNA-Seq was performed. Expression levels of Uhrf1 splicing variants in the control or shSet1a-expressing retinas are shown. Values are average of 3 or 4 samples of RNA-Seq (GSE154498) data with standard deviation. *q
    Full Length Uhrf1 Ha, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/full length uhrf1 ha/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    full length uhrf1 ha - by Bioz Stars, 2021-07
    94/100 stars
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    Image Search Results


    Expression of Uhrf1 transcripts was decreased by the expression of shSetd1. ( A ) Schematic representation of alternative splicing products of Uhrf1 . Adapted from the schematic data of asia.ensembl.org. ( B ) . Transition of expression level of transcripts of Uhrf1 during mouse retinal development from RNA-Seq data of whole mouse retina (GSE87064). 32 ( C ) Retinas at E17 were transfected with control or shSetd1a plasmids with an EGFP expression vector and cultured 2 days. EGFP positive-cells were purified, and RNA-Seq was performed. Expression levels of Uhrf1 splicing variants in the control or shSet1a-expressing retinas are shown. Values are average of 3 or 4 samples of RNA-Seq (GSE154498) data with standard deviation. *q

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Setd1a Plays Pivotal Roles for the Survival and Proliferation of Retinal Progenitors via Histone Modifications of Uhrf1

    doi: 10.1167/iovs.62.6.1

    Figure Lengend Snippet: Expression of Uhrf1 transcripts was decreased by the expression of shSetd1. ( A ) Schematic representation of alternative splicing products of Uhrf1 . Adapted from the schematic data of asia.ensembl.org. ( B ) . Transition of expression level of transcripts of Uhrf1 during mouse retinal development from RNA-Seq data of whole mouse retina (GSE87064). 32 ( C ) Retinas at E17 were transfected with control or shSetd1a plasmids with an EGFP expression vector and cultured 2 days. EGFP positive-cells were purified, and RNA-Seq was performed. Expression levels of Uhrf1 splicing variants in the control or shSet1a-expressing retinas are shown. Values are average of 3 or 4 samples of RNA-Seq (GSE154498) data with standard deviation. *q

    Article Snippet: The full-length Uhrf1_HA was purchased from Sino Biological (MG53591-CY, China), and shUhrf1‐resistant Uhrf1 was made by inverse PCR using the KOD‐Plus‐Mutagenesis Kit (Toyobo).

    Techniques: Expressing, RNA Sequencing Assay, Transfection, Plasmid Preparation, Cell Culture, Purification, Standard Deviation

    Expression of shUhrf1 perturbed retinal development. Plasmids encoding control, shUhrf1, UHRF1, or shSetd1a and EGFP expression plasmids were electroporated into mouse retina at E14 (B, C) or E17 (A, D – F) . Combination of transfected plasmids are indicated in the figures. ( A ) After sorting EGFP-positive cells, total RNA was purified and served to RT-qPCR. The averages of three independent samples with standard deviations are shown. * p

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Setd1a Plays Pivotal Roles for the Survival and Proliferation of Retinal Progenitors via Histone Modifications of Uhrf1

    doi: 10.1167/iovs.62.6.1

    Figure Lengend Snippet: Expression of shUhrf1 perturbed retinal development. Plasmids encoding control, shUhrf1, UHRF1, or shSetd1a and EGFP expression plasmids were electroporated into mouse retina at E14 (B, C) or E17 (A, D – F) . Combination of transfected plasmids are indicated in the figures. ( A ) After sorting EGFP-positive cells, total RNA was purified and served to RT-qPCR. The averages of three independent samples with standard deviations are shown. * p

    Article Snippet: The full-length Uhrf1_HA was purchased from Sino Biological (MG53591-CY, China), and shUhrf1‐resistant Uhrf1 was made by inverse PCR using the KOD‐Plus‐Mutagenesis Kit (Toyobo).

    Techniques: Expressing, Transfection, Purification, Quantitative RT-PCR