anti nf200 antibody  (Boster Bio)

Journal: Neural Regeneration Research

Article Title: Effect of the combination of high-frequency repetitive magnetic stimulation and neurotropin on injured sciatic nerve regeneration in rats

doi: 10.4103/1673-5374.264461

Figure Lengend Snippet: Effects of HF-rMS and NTP on morphological analysis of regenerated nerve. (A) Representative images of the regenerated nerve: proximal: the myelinated fibers stained by toluidine blue in the transverse section of proximal end (toluidine blue staining); distal: the myelinated fibers stained by toluidine blue in the transverse section of distal end (toluidine blue staining); medial: the axons stained by NF200 in the longitudinal of intermediate regime (immunohistochemical staining with NF200). Scale bars: 100 μm. (B) The number of proximal myelinated fibers in the four groups. (C) The number of distal myelinated fibers in the four groups. (D) The total length of NF200-positive axons in the four groups; ** P

Article Snippet: After the removal of excess liquid, sections were incubated in 50 μL anti-NF200 antibody (BM0100; Boster, Wuhan, China) overnight at 4°C, rewarmed at 37°C for 45 minutes, washed in PBS three times for 5 minutes each time, incubated in 50 μL biotin-labeled goat anti-mouse secondary antibodies (SA1020; Boster) at 37°C for 30 minutes and washed in PBS three times for 5 minutes each.

Techniques: Staining, Immunohistochemistry

Journal: Molecular Pain

Article Title: MicroRNA-30b regulates expression of the sodium channel Nav1.7 in nerve injury-induced neuropathic pain in the rat

doi: 10.1177/1744806916671523

Figure Lengend Snippet: Subpopulation distribution of miR-30b-containing neurons in DRG of naive rats. In situ hybridization of miR-30b and immunofluorescence staining of CGRP (a–c), IB4 (d–f), and NF200 (g–i) show that miR-30b was colocalized with nociceptive neuronal and non-nociceptive neurons marker. n = 3, Scale bars: 20 µm.

Article Snippet: The slices were incubated with 10% normal goat serum to block the non-specific binding of immunoglobulins and then incubated with primary antibodies diluted in blocking solution at 4℃ overnight (mouse anti-Nav1.7 (Abcam, lot number GR51762-1, catalog number ab62758, 1:200), mouse anti-NF200 (Boster, BM0100, 1:100), and rat anti-calcitonin gene-related peptide (CGRP) (Sigma, C8198, 1:100)).

Techniques: In Situ Hybridization, Immunofluorescence, Staining, Marker

Journal: Molecular Pain

Article Title: MicroRNA-30b regulates expression of the sodium channel Nav1.7 in nerve injury-induced neuropathic pain in the rat

doi: 10.1177/1744806916671523

Figure Lengend Snippet: Subpopulation distribution of Nav1.7 protein-containing neurons and double labeling of miR-30b with Nav1.7 protein in DRG of naive rats. Neurons were double labeled with Nav1.7 and CGRP (a–c), IB4 (d–f), or NF200 (g–i). Representative examples showing that most miR-30b labeled neurons in the DRG express Nav1.7 protein (j–l). n = 3 rats. Scale bar: 20 µm.

Article Snippet: The slices were incubated with 10% normal goat serum to block the non-specific binding of immunoglobulins and then incubated with primary antibodies diluted in blocking solution at 4℃ overnight (mouse anti-Nav1.7 (Abcam, lot number GR51762-1, catalog number ab62758, 1:200), mouse anti-NF200 (Boster, BM0100, 1:100), and rat anti-calcitonin gene-related peptide (CGRP) (Sigma, C8198, 1:100)).

Techniques: Labeling

Journal: Experimental and Therapeutic Medicine

Article Title: Effective robotic assistive pattern of treadmill training for spinal cord injury in a rat model

doi: 10.3892/etm.2018.5822

Figure Lengend Snippet: The representative images of NF200 + expression under a 20X objective lens in the dorsal horn of the spinal cord lesion epicenter after 3 weeks of BWSTT. (A-D) NF200 + expression for the sham, sedentary, NRSPA, and MA groups, respectively, and the green fluorescence in the white boxes is typical NF200 + expression. (A) The extensive axonal connection is visible in the sham group, while in (B) the NF200 + expression is scattered, short, and thin in the sedentary group. Scattered, short and dotted NF200 + expression with limited strong positive expression can been seen in the NRSPA (C) and MA groups (D); the differences between the two groups are not obvious, as shown in the image. NF200, neurofilament 200; BWSTT, body-weight-supported treadmill training; NRSPA, normal rat stepping pattern assistance; MA, manual assistance.

Article Snippet: The primary antibody was neurofilament 200 (NF200) antibody (BM0100) diluted 1:200 and the secondary antibody was anti-GAPDH rabbit monoclonal antibody (M00227) (both from BosterBio, Pleasanton, CA, USA) diluted 1:1,000.

Techniques: Expressing, Fluorescence

Journal: Frontiers in Medicine

Article Title: Ozone Exposure Induces Metabolic Disorders and NAD+ Depletion Through PARP1 Activation in Spinal Cord Neurons

doi: 10.3389/fmed.2020.617321

Figure Lengend Snippet: Immunofluorescence identification of SCNs. (A) Cell nuclei are identified with DAPI. (B) SCNs are stained with the anti-NF200 monoclonal antibody. More than 90% of the cultured cells show positive expression of NF200 on the day 7 of ex vivo culture. The number of NF200-positive neurons are counted per area and expressed as percentage of total number of cells (Scale bar: 40 μm).

Article Snippet: The cells were fixed with paraformaldehyde for 15 min, permeabilized for 5 min at the room temperature with 0.5% Triton X-100, and blocked with goat serum at room temperature for 1 h. The cells were incubated with anti-NF200 (Boster, BM0100, 1:100) overnight at 4°C.

Techniques: Immunofluorescence, Staining, Cell Culture, Expressing, Ex Vivo