gfap  (Boster Bio)


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    Name:
    Anti GFAP Antibody
    Description:

    Catalog Number:
    MA1045
    Price:
    99.0
    Category:
    Primary Antibodies
    Reactivity:
    Human Pig Rat
    Applications:
    IF, IHC-P, IHC-F, WB
    Immunogen:
    GFAP from pig spinal cord.
    Host:
    Mouse
    Isotype:
    Mouse IgG1
    Buy from Supplier


    Structured Review

    Boster Bio gfap
    Anti GFAP Antibody

    https://www.bioz.com/result/gfap/product/Boster Bio
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gfap - by Bioz Stars, 2021-06
    93/100 stars

    Images

    1) Product Images from "Reversion of malignant phenotypes of human glioblastoma cells by β-elemene through β-catenin-mediated regulation of stemness-, differentiation- and epithelial-to-mesenchymal transition-related molecules"

    Article Title: Reversion of malignant phenotypes of human glioblastoma cells by β-elemene through β-catenin-mediated regulation of stemness-, differentiation- and epithelial-to-mesenchymal transition-related molecules

    Journal: Journal of Translational Medicine

    doi: 10.1186/s12967-015-0727-2

    β -Elemene regulation of stemness-, differentiation- and EMT-related effectors in vivo. a Western blot assays were performed on the aforementioned glioblastoma xenografts in the β -elemene- and NaCl-treated groups. Expression levels of CD133, ABCG2, N-cadherin and β -catenin were significantly downregulated and expression levels of E-cadherin, GFAP, Notch1 and SHH were upregulated by β -elemene. Interestingly, the expression level of vimentin in the β -elemene-treated group was significantly lower than that in the NaCl-treated group; this result was opposite that for the in vitro procedure. b The results are representative of three independent experiments, and the values are presented as the mean ± SD (* p
    Figure Legend Snippet: β -Elemene regulation of stemness-, differentiation- and EMT-related effectors in vivo. a Western blot assays were performed on the aforementioned glioblastoma xenografts in the β -elemene- and NaCl-treated groups. Expression levels of CD133, ABCG2, N-cadherin and β -catenin were significantly downregulated and expression levels of E-cadherin, GFAP, Notch1 and SHH were upregulated by β -elemene. Interestingly, the expression level of vimentin in the β -elemene-treated group was significantly lower than that in the NaCl-treated group; this result was opposite that for the in vitro procedure. b The results are representative of three independent experiments, and the values are presented as the mean ± SD (* p

    Techniques Used: In Vivo, Western Blot, Expressing, In Vitro

    β -Elemene regulated the expression of stemness markers and differentiation-related effectors in glioblastoma cells. a CD133 + and ABCG2 + cells were sparsely distributed throughout both G1 and G2 tissues, and both CD133 and ABCG2 were localized to both the cytoplasm and cytomembrane. The expression of GFAP was higher in the G1 tissue than in the G2 tissue. b β -Elemene decreased the expression levels of CD133 and ABCG2 and increased the expression levels of GFAP, Notch1 and SHH in a dose-dependent manner. c The results of ( b ) were semi-quantitatively estimated using Gel-Pro Analyzer 4.0 software and are illustrated graphically. The results are representative of three independent experiments, and the values are presented as the mean ± SD (* p
    Figure Legend Snippet: β -Elemene regulated the expression of stemness markers and differentiation-related effectors in glioblastoma cells. a CD133 + and ABCG2 + cells were sparsely distributed throughout both G1 and G2 tissues, and both CD133 and ABCG2 were localized to both the cytoplasm and cytomembrane. The expression of GFAP was higher in the G1 tissue than in the G2 tissue. b β -Elemene decreased the expression levels of CD133 and ABCG2 and increased the expression levels of GFAP, Notch1 and SHH in a dose-dependent manner. c The results of ( b ) were semi-quantitatively estimated using Gel-Pro Analyzer 4.0 software and are illustrated graphically. The results are representative of three independent experiments, and the values are presented as the mean ± SD (* p

    Techniques Used: Expressing, Software

    Related Articles

    Western Blot:

    Article Title: Reversion of malignant phenotypes of human glioblastoma cells by β-elemene through β-catenin-mediated regulation of stemness-, differentiation- and epithelial-to-mesenchymal transition-related molecules
    Article Snippet: .. Antibodies against CD133, ABCG2 and GFAP that were used for Western blot and immunohistochemistry analyses were purchased from Boster Co., Ltd. (Wuhan, China). .. GAPDH antibody was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA), and the antibodies against Notch1, SHH, β -catenin, vimentin, E-cadherin and N-cadherin were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA).

    Immunohistochemistry:

    Article Title: Reversion of malignant phenotypes of human glioblastoma cells by β-elemene through β-catenin-mediated regulation of stemness-, differentiation- and epithelial-to-mesenchymal transition-related molecules
    Article Snippet: .. Antibodies against CD133, ABCG2 and GFAP that were used for Western blot and immunohistochemistry analyses were purchased from Boster Co., Ltd. (Wuhan, China). .. GAPDH antibody was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA), and the antibodies against Notch1, SHH, β -catenin, vimentin, E-cadherin and N-cadherin were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA).

    Cell Culture:

    Article Title: RGMa mediates reactive astrogliosis and glial scar formation through TGFβ1/Smad2/3 signaling after stroke
    Article Snippet: On the following day, the sections were washed using PBS and incubated with appropriate secondary antibodies conjugated with Alexa Fluor 488 (1:200, A-21206 Thermo Fisher) or 555 (1:200, A0460 Beyotime; or 1:300, A-21432 Thermo Fisher) for 1 h at 37 °C. .. For cultured astrocyte staining, cells were fixed with 100% methanol for 10 min. After washing with PBS, cells were blocked by 10% normal donkey serum for 1 h at 37 °C and incubated overnight at 4 °C with primary antibodies including anti-RGMa (1:50, ab169761 Abcam) and anti-GFAP (1:100, BM0055 Boster). .. Cells were washed and for 1 h at 37 °C incubation with secondary antibodies conjugated with Alexa Fluor 488 (1:200, A-21206 Thermo Fisher) or 555 (1:200, A0460 Beyotime).

    Staining:

    Article Title: RGMa mediates reactive astrogliosis and glial scar formation through TGFβ1/Smad2/3 signaling after stroke
    Article Snippet: On the following day, the sections were washed using PBS and incubated with appropriate secondary antibodies conjugated with Alexa Fluor 488 (1:200, A-21206 Thermo Fisher) or 555 (1:200, A0460 Beyotime; or 1:300, A-21432 Thermo Fisher) for 1 h at 37 °C. .. For cultured astrocyte staining, cells were fixed with 100% methanol for 10 min. After washing with PBS, cells were blocked by 10% normal donkey serum for 1 h at 37 °C and incubated overnight at 4 °C with primary antibodies including anti-RGMa (1:50, ab169761 Abcam) and anti-GFAP (1:100, BM0055 Boster). .. Cells were washed and for 1 h at 37 °C incubation with secondary antibodies conjugated with Alexa Fluor 488 (1:200, A-21206 Thermo Fisher) or 555 (1:200, A0460 Beyotime).

    Incubation:

    Article Title: RGMa mediates reactive astrogliosis and glial scar formation through TGFβ1/Smad2/3 signaling after stroke
    Article Snippet: On the following day, the sections were washed using PBS and incubated with appropriate secondary antibodies conjugated with Alexa Fluor 488 (1:200, A-21206 Thermo Fisher) or 555 (1:200, A0460 Beyotime; or 1:300, A-21432 Thermo Fisher) for 1 h at 37 °C. .. For cultured astrocyte staining, cells were fixed with 100% methanol for 10 min. After washing with PBS, cells were blocked by 10% normal donkey serum for 1 h at 37 °C and incubated overnight at 4 °C with primary antibodies including anti-RGMa (1:50, ab169761 Abcam) and anti-GFAP (1:100, BM0055 Boster). .. Cells were washed and for 1 h at 37 °C incubation with secondary antibodies conjugated with Alexa Fluor 488 (1:200, A-21206 Thermo Fisher) or 555 (1:200, A0460 Beyotime).

    Article Title: RGMa mediates reactive astrogliosis and glial scar formation through TGFβ1/Smad2/3 signaling after stroke
    Article Snippet: .. Then the sections were incubated overnight at 4 °C with specific primary antibodies as follows: anti-GFAP (1:100, BM0055 Boster, Wuhan, China), anti-RGMa (1:50, ab26287 Abcam), anti-NeuN (1:50, MAB377 Millipore), anti-Nestin (1:100, ab11306 Abcam), anti-CC1 (1:50, ab16794 Abcam), anti-NG2 (1:100, ab50009 Abcam), anti-Iba1 (1:50, NB100-1028 Novus, Littleton, CO, USA), anti-CD31 (1:50, ab64543 Abcam), anti-αSMA (1:50, ab21027 Abcam), anti-fibronectin (1:50, 610077 BD Biosciences, Franklin Lakes, NJ, USA), anti-collagen I (1:100, ab90395 Abcam), anti-neurocan (1:100, N0913 Sigma-Aldrich), and anti-phosphacan (1:100, P8874 Sigma-Aldrich). .. On the following day, the sections were washed using PBS and incubated with appropriate secondary antibodies conjugated with Alexa Fluor 488 (1:200, A-21206 Thermo Fisher) or 555 (1:200, A0460 Beyotime; or 1:300, A-21432 Thermo Fisher) for 1 h at 37 °C.

    Article Title: Bone marrow-derived mesenchymal stem cells differentiate into nerve-like cells in vitro after transfection with brain-derived neurotrophic factor gene
    Article Snippet: Detection of BDNF, nestin, NSE, and GFAP by immunocytochemistry. .. Immunocytochemistry was performed using the SABC method (Shi et al. ): the culture medium was removed from adherent cells, and the cells were then fixed in 4% paraformaldehyde, incubated in a mixture of 30% hydrogen peroxide and pure methanol, incubated in goat serum, and then incubated with the anti-BDNF, anti-nestin, anti-NSE, and anti-GFAP (1:300 dilution for all; Wuhan Boster, Wuhan, China) primary antibodies overnight in a 4°C refrigerator. .. The following day, incubation with the HRP-labeled rabbit anti-mouse secondary antibody (Wuhan Boster, Wuhan, China) was performed after rewarming of the samples, followed by staining with streptavidin-biotin complex (SABC; Wuhan Boster) and 3,3′-diaminobenzidine (DAB; Wuhan Boster) and counterstaining with hematoxylin.

    Article Title: Label-retaining assay enriches tumor-initiating cells in glioblastoma spheres cultivated in serum-free medium
    Article Snippet: For immunohistochemistry, the dewaxed and hydrated sections were incubated in a warm water bath at 95°C for 20 min in neutral (pH 7.0) antigen retrieval solution (Dako, Glostrup, Denmark). .. Subsequently, the sections were incubated with the following primary antibodies overnight at 4°C: rabbit anti-human Nestin (dilution, 1:10; Boster Systems, Inc.), mouse anti-human GFAP (dilution, 1:10; Boster Systems, Inc.), mouse anti-human Ki67 (dilution, 1:10; Boster Systems, Inc.) or mouse anti-human CD31 (dilution, 1:10; Boster Systems, Inc.). .. The subsequent procedures were performed according to the manufacturer's protocol for a mouse- or rabbit-specific HRP/AEC Polymer Detection Immunohistochemistry kit (Abcam).

    Article Title: Electroacupuncture Suppresses the NF-κB Signaling Pathway by Upregulating Cylindromatosis to Alleviate Inflammatory Injury in Cerebral Ischemia/Reperfusion Rats
    Article Snippet: .. Sections were subsequently incubated with prepared primary antibodies at 4°C overnight as follows: Anti-NeuN mouse (labeled neurons, MAB377, Millipore, 1:100), anti-GFAP mouse (labeled astrocytes, BM0055, Boster, 1:100), anti-Iba1 goat (to label microglia, NB100-1028SS, Novus, 1:50), anti-CYLD rabbit (11110-1-AP, Proteintech, 1:100), anti-CYLD mouse (SC-74435, Santa Cruz, 1:100), anti-NF-κB p65 rabbit (#8242, Cell Signaling Technology, 1:50) and anti-CX3CL1 rabbit (ab25088, Abcam, 1:100). .. After rewarming for 1 h at 37°C, sections were incubated with secondary antibodies for 1 h at 37°C in the dark: Alexa Fluor 594-conjugated goat anti-rabbit IgG (H + L) (SA00006-4, Proteintech, 1:200), Alexa Fluor 594-conjugated donkey anti-rabbit IgG (H + L) (SA00006-8, Proteintech, 1:200), Alexa Fluor 488-conjugated goat anti-mouse IgG (H + L) (SA00006-1, Proteintech, 1:200), FITC-conjugated Affinipure donkey anti-goat IgG (H + L) (SA00003-3, Proteintech, 1:200).

    Immunocytochemistry:

    Article Title: Bone marrow-derived mesenchymal stem cells differentiate into nerve-like cells in vitro after transfection with brain-derived neurotrophic factor gene
    Article Snippet: Detection of BDNF, nestin, NSE, and GFAP by immunocytochemistry. .. Immunocytochemistry was performed using the SABC method (Shi et al. ): the culture medium was removed from adherent cells, and the cells were then fixed in 4% paraformaldehyde, incubated in a mixture of 30% hydrogen peroxide and pure methanol, incubated in goat serum, and then incubated with the anti-BDNF, anti-nestin, anti-NSE, and anti-GFAP (1:300 dilution for all; Wuhan Boster, Wuhan, China) primary antibodies overnight in a 4°C refrigerator. .. The following day, incubation with the HRP-labeled rabbit anti-mouse secondary antibody (Wuhan Boster, Wuhan, China) was performed after rewarming of the samples, followed by staining with streptavidin-biotin complex (SABC; Wuhan Boster) and 3,3′-diaminobenzidine (DAB; Wuhan Boster) and counterstaining with hematoxylin.

    Labeling:

    Article Title: Electroacupuncture Suppresses the NF-κB Signaling Pathway by Upregulating Cylindromatosis to Alleviate Inflammatory Injury in Cerebral Ischemia/Reperfusion Rats
    Article Snippet: .. Sections were subsequently incubated with prepared primary antibodies at 4°C overnight as follows: Anti-NeuN mouse (labeled neurons, MAB377, Millipore, 1:100), anti-GFAP mouse (labeled astrocytes, BM0055, Boster, 1:100), anti-Iba1 goat (to label microglia, NB100-1028SS, Novus, 1:50), anti-CYLD rabbit (11110-1-AP, Proteintech, 1:100), anti-CYLD mouse (SC-74435, Santa Cruz, 1:100), anti-NF-κB p65 rabbit (#8242, Cell Signaling Technology, 1:50) and anti-CX3CL1 rabbit (ab25088, Abcam, 1:100). .. After rewarming for 1 h at 37°C, sections were incubated with secondary antibodies for 1 h at 37°C in the dark: Alexa Fluor 594-conjugated goat anti-rabbit IgG (H + L) (SA00006-4, Proteintech, 1:200), Alexa Fluor 594-conjugated donkey anti-rabbit IgG (H + L) (SA00006-8, Proteintech, 1:200), Alexa Fluor 488-conjugated goat anti-mouse IgG (H + L) (SA00006-1, Proteintech, 1:200), FITC-conjugated Affinipure donkey anti-goat IgG (H + L) (SA00003-3, Proteintech, 1:200).

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    Boster Bio anti gfap
    Immunocytochemical analysis of BMSCs and <t>BDNF-BMSCs</t> (brown staining highlighted by arrows , magnification ×200). ( A1 – A4 ) Non-induced BMSCs expressed low levels of BDNF, nestin, NSE, and <t>GFAP.</t> ( B1 – B4 ) Low levels of BDNF, nestin, NSE, and GFAP were also detected in induced BMSCs. ( C1 – C4 ) The number of cells positively co-stained with BDNF, nestin, NSE, and GFAP in non-induced BDNF-BMSCs was significantly higher than that in BMSCs. Over 98% of non-induced BDNF-BMSCs were positively stained for BDNF. ( D1 – D4 ) Over 98% of induced BDNF-BMSCs were positively stained for BDNF. More cells positively co-stained for nestin, NSE, and GFAP were detected in induced BDNF-BMSCs than in non-induced BDNF-BMSCs. ( E ) Rates of positive immunostaining in BMSCs and BDNF-BMSCs, as determined by immunocytochemistry for BDNF, nestin, NSE, and GFAP (%; mean ± SD). # P
    Anti Gfap, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti gfap/product/Boster Bio
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti gfap - by Bioz Stars, 2021-06
    93/100 stars
      Buy from Supplier

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    Immunocytochemical analysis of BMSCs and BDNF-BMSCs (brown staining highlighted by arrows , magnification ×200). ( A1 – A4 ) Non-induced BMSCs expressed low levels of BDNF, nestin, NSE, and GFAP. ( B1 – B4 ) Low levels of BDNF, nestin, NSE, and GFAP were also detected in induced BMSCs. ( C1 – C4 ) The number of cells positively co-stained with BDNF, nestin, NSE, and GFAP in non-induced BDNF-BMSCs was significantly higher than that in BMSCs. Over 98% of non-induced BDNF-BMSCs were positively stained for BDNF. ( D1 – D4 ) Over 98% of induced BDNF-BMSCs were positively stained for BDNF. More cells positively co-stained for nestin, NSE, and GFAP were detected in induced BDNF-BMSCs than in non-induced BDNF-BMSCs. ( E ) Rates of positive immunostaining in BMSCs and BDNF-BMSCs, as determined by immunocytochemistry for BDNF, nestin, NSE, and GFAP (%; mean ± SD). # P

    Journal: In Vitro Cellular & Developmental Biology. Animal

    Article Title: Bone marrow-derived mesenchymal stem cells differentiate into nerve-like cells in vitro after transfection with brain-derived neurotrophic factor gene

    doi: 10.1007/s11626-015-9875-1

    Figure Lengend Snippet: Immunocytochemical analysis of BMSCs and BDNF-BMSCs (brown staining highlighted by arrows , magnification ×200). ( A1 – A4 ) Non-induced BMSCs expressed low levels of BDNF, nestin, NSE, and GFAP. ( B1 – B4 ) Low levels of BDNF, nestin, NSE, and GFAP were also detected in induced BMSCs. ( C1 – C4 ) The number of cells positively co-stained with BDNF, nestin, NSE, and GFAP in non-induced BDNF-BMSCs was significantly higher than that in BMSCs. Over 98% of non-induced BDNF-BMSCs were positively stained for BDNF. ( D1 – D4 ) Over 98% of induced BDNF-BMSCs were positively stained for BDNF. More cells positively co-stained for nestin, NSE, and GFAP were detected in induced BDNF-BMSCs than in non-induced BDNF-BMSCs. ( E ) Rates of positive immunostaining in BMSCs and BDNF-BMSCs, as determined by immunocytochemistry for BDNF, nestin, NSE, and GFAP (%; mean ± SD). # P

    Article Snippet: Immunocytochemistry was performed using the SABC method (Shi et al. ): the culture medium was removed from adherent cells, and the cells were then fixed in 4% paraformaldehyde, incubated in a mixture of 30% hydrogen peroxide and pure methanol, incubated in goat serum, and then incubated with the anti-BDNF, anti-nestin, anti-NSE, and anti-GFAP (1:300 dilution for all; Wuhan Boster, Wuhan, China) primary antibodies overnight in a 4°C refrigerator.

    Techniques: Staining, Immunostaining, Immunocytochemistry

    mRNA expression of BMSCs and BDNF-BMSCs. BDNF, nestin, NSE, and GFAP mRNA expression of BMSCs and BDNF-BMSCs determined by RT-PCR (mean ± SD). # P

    Journal: In Vitro Cellular & Developmental Biology. Animal

    Article Title: Bone marrow-derived mesenchymal stem cells differentiate into nerve-like cells in vitro after transfection with brain-derived neurotrophic factor gene

    doi: 10.1007/s11626-015-9875-1

    Figure Lengend Snippet: mRNA expression of BMSCs and BDNF-BMSCs. BDNF, nestin, NSE, and GFAP mRNA expression of BMSCs and BDNF-BMSCs determined by RT-PCR (mean ± SD). # P

    Article Snippet: Immunocytochemistry was performed using the SABC method (Shi et al. ): the culture medium was removed from adherent cells, and the cells were then fixed in 4% paraformaldehyde, incubated in a mixture of 30% hydrogen peroxide and pure methanol, incubated in goat serum, and then incubated with the anti-BDNF, anti-nestin, anti-NSE, and anti-GFAP (1:300 dilution for all; Wuhan Boster, Wuhan, China) primary antibodies overnight in a 4°C refrigerator.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction

    β -Elemene regulation of stemness-, differentiation- and EMT-related effectors in vivo. a Western blot assays were performed on the aforementioned glioblastoma xenografts in the β -elemene- and NaCl-treated groups. Expression levels of CD133, ABCG2, N-cadherin and β -catenin were significantly downregulated and expression levels of E-cadherin, GFAP, Notch1 and SHH were upregulated by β -elemene. Interestingly, the expression level of vimentin in the β -elemene-treated group was significantly lower than that in the NaCl-treated group; this result was opposite that for the in vitro procedure. b The results are representative of three independent experiments, and the values are presented as the mean ± SD (* p

    Journal: Journal of Translational Medicine

    Article Title: Reversion of malignant phenotypes of human glioblastoma cells by β-elemene through β-catenin-mediated regulation of stemness-, differentiation- and epithelial-to-mesenchymal transition-related molecules

    doi: 10.1186/s12967-015-0727-2

    Figure Lengend Snippet: β -Elemene regulation of stemness-, differentiation- and EMT-related effectors in vivo. a Western blot assays were performed on the aforementioned glioblastoma xenografts in the β -elemene- and NaCl-treated groups. Expression levels of CD133, ABCG2, N-cadherin and β -catenin were significantly downregulated and expression levels of E-cadherin, GFAP, Notch1 and SHH were upregulated by β -elemene. Interestingly, the expression level of vimentin in the β -elemene-treated group was significantly lower than that in the NaCl-treated group; this result was opposite that for the in vitro procedure. b The results are representative of three independent experiments, and the values are presented as the mean ± SD (* p

    Article Snippet: Antibodies against CD133, ABCG2 and GFAP that were used for Western blot and immunohistochemistry analyses were purchased from Boster Co., Ltd. (Wuhan, China).

    Techniques: In Vivo, Western Blot, Expressing, In Vitro

    β -Elemene regulated the expression of stemness markers and differentiation-related effectors in glioblastoma cells. a CD133 + and ABCG2 + cells were sparsely distributed throughout both G1 and G2 tissues, and both CD133 and ABCG2 were localized to both the cytoplasm and cytomembrane. The expression of GFAP was higher in the G1 tissue than in the G2 tissue. b β -Elemene decreased the expression levels of CD133 and ABCG2 and increased the expression levels of GFAP, Notch1 and SHH in a dose-dependent manner. c The results of ( b ) were semi-quantitatively estimated using Gel-Pro Analyzer 4.0 software and are illustrated graphically. The results are representative of three independent experiments, and the values are presented as the mean ± SD (* p

    Journal: Journal of Translational Medicine

    Article Title: Reversion of malignant phenotypes of human glioblastoma cells by β-elemene through β-catenin-mediated regulation of stemness-, differentiation- and epithelial-to-mesenchymal transition-related molecules

    doi: 10.1186/s12967-015-0727-2

    Figure Lengend Snippet: β -Elemene regulated the expression of stemness markers and differentiation-related effectors in glioblastoma cells. a CD133 + and ABCG2 + cells were sparsely distributed throughout both G1 and G2 tissues, and both CD133 and ABCG2 were localized to both the cytoplasm and cytomembrane. The expression of GFAP was higher in the G1 tissue than in the G2 tissue. b β -Elemene decreased the expression levels of CD133 and ABCG2 and increased the expression levels of GFAP, Notch1 and SHH in a dose-dependent manner. c The results of ( b ) were semi-quantitatively estimated using Gel-Pro Analyzer 4.0 software and are illustrated graphically. The results are representative of three independent experiments, and the values are presented as the mean ± SD (* p

    Article Snippet: Antibodies against CD133, ABCG2 and GFAP that were used for Western blot and immunohistochemistry analyses were purchased from Boster Co., Ltd. (Wuhan, China).

    Techniques: Expressing, Software

    Brain-specific serine/threonine-protein kinase 1 (SAD-B) is localized in the epileptic brain. A , Immunofluorescence labelling of SAD-B (red), MAP2 (violet), and GFAP (green) in the cortex of patients with temporal lobe epilepsy (TLE) showing that SAD-B was co-localized with MAP2 but not with GFAP. Scale bar: 50 μm (400×). B , Immunofluorescence labelling of SAD-B (red), MAP2 (violet), and GFAP (green) in the CA1 region of the hippocampus or cortex of an epileptic rat showing that SAD-B was co-localized with MAP2, but not with GFAP. Scale bar: 50 μm (400×). White arrows: SAD-B; yellow arrows: GFAP.

    Journal: Brazilian Journal of Medical and Biological Research

    Article Title: SAD-B modulates epileptic seizure by regulating AMPA receptors in patients with temporal lobe epilepsy and in the PTZ-induced epileptic model

    doi: 10.1590/1414-431X20199175

    Figure Lengend Snippet: Brain-specific serine/threonine-protein kinase 1 (SAD-B) is localized in the epileptic brain. A , Immunofluorescence labelling of SAD-B (red), MAP2 (violet), and GFAP (green) in the cortex of patients with temporal lobe epilepsy (TLE) showing that SAD-B was co-localized with MAP2 but not with GFAP. Scale bar: 50 μm (400×). B , Immunofluorescence labelling of SAD-B (red), MAP2 (violet), and GFAP (green) in the CA1 region of the hippocampus or cortex of an epileptic rat showing that SAD-B was co-localized with MAP2, but not with GFAP. Scale bar: 50 μm (400×). White arrows: SAD-B; yellow arrows: GFAP.

    Article Snippet: The primary antibodies included mouse anti-SAD-B (diluted 1:100, Cat. No. ab206298; Abcam, UK), chicken anti-microtubule-associated protein 2 (MAP2) (1:200, Cat. No. ab5392; Abcam), and rabbit anti-glial fibrillary acidic protein (GFAP) (1:50, Cat. No. BM0055; Boster, China).

    Techniques: Immunofluorescence

    DiI-retaining cells demonstrate significantly increased tumorigenicity in NOD/SCID mice compared with DiI-negative cells. Representative images of (A-a) Nestin (left panel); (A-b) GFAP (left panel); (A-c) Ki67 (left panel) and (A-d) cluster of differentiation 31 (left panel) immunohistochemical staining in the xenografts from NOD/SCID mice implanted with DiI-retaining or DiI-negative cells. Scale bar=100 µm. Quantification of the cells positive for (A-a) Nestin (right panel), (A-b) GFAP (right panel) and (A-c) Ki67 (right panel). *P

    Journal: Oncology Letters

    Article Title: Label-retaining assay enriches tumor-initiating cells in glioblastoma spheres cultivated in serum-free medium

    doi: 10.3892/ol.2016.4690

    Figure Lengend Snippet: DiI-retaining cells demonstrate significantly increased tumorigenicity in NOD/SCID mice compared with DiI-negative cells. Representative images of (A-a) Nestin (left panel); (A-b) GFAP (left panel); (A-c) Ki67 (left panel) and (A-d) cluster of differentiation 31 (left panel) immunohistochemical staining in the xenografts from NOD/SCID mice implanted with DiI-retaining or DiI-negative cells. Scale bar=100 µm. Quantification of the cells positive for (A-a) Nestin (right panel), (A-b) GFAP (right panel) and (A-c) Ki67 (right panel). *P

    Article Snippet: Subsequently, the sections were incubated with the following primary antibodies overnight at 4°C: rabbit anti-human Nestin (dilution, 1:10; Boster Systems, Inc.), mouse anti-human GFAP (dilution, 1:10; Boster Systems, Inc.), mouse anti-human Ki67 (dilution, 1:10; Boster Systems, Inc.) or mouse anti-human CD31 (dilution, 1:10; Boster Systems, Inc.).

    Techniques: Mouse Assay, Immunohistochemistry, Staining