anti cdk6  (Boster Bio)

Journal: British Journal of Cancer

Article Title: Circular RNA 0000096 affects cell growth and migration in gastric cancer

doi: 10.1038/bjc.2016.451

Figure Lengend Snippet: Expression levels of cell cycle-related and migration-related proteins in human normal gastric epithelial and human gastric cancer cell lines following knockdown of hsa_circ_0000096. Representative western blot results of cycle-related proteins cyclin D1 and CDK6 ( A , B ). Representative western blot results of migration-related proteins MMP-2 and MMP-9 ( C , D ). Data are presented as the mean±s.d., n =3. NC, negative control. * P

Article Snippet: Immunoblotting of the membrane was performed using the following primary antibodies: anti-CDK6 (Boster, Wuhan, China), anti-cyclin D1 (Affinity, Cincinnati, OH, USA), anti-MMP-2 (Boster), anti-MMP-9 (Boster) and anti-β -actin (4 A Biotech, Beijing, China).

Techniques: Expressing, Migration, Western Blot, Negative Control

Journal: British Journal of Cancer

Article Title: Circular RNA 0000096 affects cell growth and migration in gastric cancer

doi: 10.1038/bjc.2016.451

Figure Lengend Snippet: Knockdown of hsa_circ_0000096 suppresses cell cycle-related and migration-related protein expression. The results of cell cycle-related and migration-related protein levels in xenografts nude mouse models ( A ). Representative expression levels of cyclin D1, CDK6, MMP-2 and MMP-9 ( B ). Data are presented as the mean±s.d., n =3. NC, negative control. * P

Article Snippet: Immunoblotting of the membrane was performed using the following primary antibodies: anti-CDK6 (Boster, Wuhan, China), anti-cyclin D1 (Affinity, Cincinnati, OH, USA), anti-MMP-2 (Boster), anti-MMP-9 (Boster) and anti-β -actin (4 A Biotech, Beijing, China).

Techniques: Migration, Expressing, Negative Control

Journal: Frontiers in Oncology

Article Title: Combined Anti-Cancer Effects of Platycodin D and Sorafenib on Androgen-Independent and PTEN-Deficient Prostate Cancer

doi: 10.3389/fonc.2021.648985

Figure Lengend Snippet: PD promotes the anti-tumor effects of sorafenib in PC3 cells. (A) . The effects of PD, sorafenib and PD plus sorafenib on cell viability. (B, C) . The changes in the cell membrane and nucleus after treatment with PD alone, sorafenib (Sor) alone or PD plus sorafenib. (D) . The induction of apoptosis by PD, Sor and PD + Sor. (E) . The protein expression levels of Caspase 3, C-Caspase 3, PARP and C-PARP were examined after cells were treated with 10 μM PD alone, 10 μM sorafenib alone or PD plus sorafenib for 6 h. (F) . The ability of cells to achieve colony growth was assessed after treatment with PD alone, sorafenib alone or PD plus sorafenib for 10 days. (G) . The proliferation of cells was monitored using the CFDA SE assay after treatment with PD alone, sorafenib alone or PD plus sorafenib for 5 days. (H, I) . The cell cycle distribution of PC3 cells following treatment with PD alone, sorafenib alone or PD plus sorafenib for 24 h after pre-treatment with (H) or without (I) 2 mM thymidine. (J) . Changes in the protein expression levels of CDK4, CDK6 and cyclin D1 after treatment with 5 μM PD alone, 2.5 μM sorafenib alone or PD plus sorafenib for 24 h. * p

Article Snippet: The anti-CDK4, anti-CDK6 and anti-cyclin D antibodies were obtained from Boster Biological Technology, Inc. (Wuhan, China).

Techniques: Expressing

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Long noncoding RNA NEAT1 promotes laryngeal squamous cell cancer through regulating miR-107/CDK6 pathway

doi: 10.1186/s13046-016-0297-z

Figure Lengend Snippet: NEAT1 regulates CDK6 through modulating miR-107. a TargetScan database predicated CDK6 as a target of miR-107. b miR-107 decreased luciferase activity of 3′UTR but not that of 3′UTR-NC and 3′UTR-MU. miR-NC indicated scramble miRNA used as the negative control for miR-107. ** P

Article Snippet: Western blot analysis Hep-2 cells were collected and CDK6 expression was detected as described previously using CDK6 antibody (1:400 dilution, Boster, Wuhan, China) [ ].

Techniques: Luciferase, Activity Assay, Negative Control