bcl 2  (Boster Bio)


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  • 95
    Name:
    Anti Bcl 2 Antibody
    Description:

    Catalog Number:
    MA1004
    Price:
    99.0
    Category:
    Primary Antibodies
    Reactivity:
    Human
    Applications:
    IHC, ICC, WB
    Immunogen:
    Synthetic peptide corresponding to residues 41-54 of the bcl-2 protein, conjugated to thyroglobulin.
    Host:
    Mouse
    Isotype:
    Mouse IgG1
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    Structured Review

    Boster Bio bcl 2
    Anti Bcl 2 Antibody

    https://www.bioz.com/result/bcl 2/product/Boster Bio
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bcl 2 - by Bioz Stars, 2021-06
    95/100 stars

    Images

    1) Product Images from "Experimental study on the therapeutic effect and underlining mechanisms of positron in pancreatic cancer cells"

    Article Title: Experimental study on the therapeutic effect and underlining mechanisms of positron in pancreatic cancer cells

    Journal: Oncotarget

    doi: 10.18632/oncotarget.18366

    Underlying mechanisms of apoptosis induced by positron in SW1990 pancreatic cancer cells Four groups cells were incubated with different concentrations 18 F-FDG (a) control group; (b) 18.5MBq/ml; (c) 37MBq/ml; (d) 74MBq/ml. (A) Real-time RT-PCR analysis for the mRNA expression of apotosis-related genes. Positron decreased Bcl-2 mRNA expression and increased Bax, Caspase-3, Caspase-9 and P53 mRNA expression in SW1990 cells in a dose-dependent manner. (B) Western blotting analysis for the Cleaved Caspase-3, Cytochrome C and Cleaved Caspase-9 protein treated with different concentrations 18 F-FDG for 24h. (C) statistical histogram of western blotting. The data were expressed as mean±SEM obtained from three independent samples. * p
    Figure Legend Snippet: Underlying mechanisms of apoptosis induced by positron in SW1990 pancreatic cancer cells Four groups cells were incubated with different concentrations 18 F-FDG (a) control group; (b) 18.5MBq/ml; (c) 37MBq/ml; (d) 74MBq/ml. (A) Real-time RT-PCR analysis for the mRNA expression of apotosis-related genes. Positron decreased Bcl-2 mRNA expression and increased Bax, Caspase-3, Caspase-9 and P53 mRNA expression in SW1990 cells in a dose-dependent manner. (B) Western blotting analysis for the Cleaved Caspase-3, Cytochrome C and Cleaved Caspase-9 protein treated with different concentrations 18 F-FDG for 24h. (C) statistical histogram of western blotting. The data were expressed as mean±SEM obtained from three independent samples. * p

    Techniques Used: Incubation, Quantitative RT-PCR, Expressing, Western Blot

    2) Product Images from "Cardioprotective effects of traditional Chinese medicine Guanmaitong on acute myocardial infarction"

    Article Title: Cardioprotective effects of traditional Chinese medicine Guanmaitong on acute myocardial infarction

    Journal: Experimental and Therapeutic Medicine

    doi: 10.3892/etm.2016.3888

    Expression of Bcl-2 and Bax in GMT-treated Sprague-Dawley rats with acute myocardial infarction. (A and B) Western Blot was used to evaluate the protein expression of Bcl-2 and Bax. (C) Immunohistochemical analysis was used to detected the expression of Bcl-2 and Bax (magnification, ×400). # P
    Figure Legend Snippet: Expression of Bcl-2 and Bax in GMT-treated Sprague-Dawley rats with acute myocardial infarction. (A and B) Western Blot was used to evaluate the protein expression of Bcl-2 and Bax. (C) Immunohistochemical analysis was used to detected the expression of Bcl-2 and Bax (magnification, ×400). # P

    Techniques Used: Expressing, Western Blot, Immunohistochemistry

    3) Product Images from "Ibutilide treatment protects against ER stress induced apoptosis by regulating calumenin expression in tunicamycin treated cardiomyocytes"

    Article Title: Ibutilide treatment protects against ER stress induced apoptosis by regulating calumenin expression in tunicamycin treated cardiomyocytes

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0173469

    Ibutilide treatment attenuates tunicamycin induced increase in Bax/ Bcl-2 ratio. Protein expression of Bax and Bcl-2 were analyzed from lysate derived from RNC treated with either tunicamycin alone (model), tunicamycin and ibutilide (treatment) or untreated cardiomyocytes (control). Representative immunoblots of lysate immunoblotted with antibodies specific for Bax or BCL-2 are shown. The quantification of normalized band densitometry of Bax was divided by that of Bcl-2 and the results are graphed. All data are shown as mean ± SE ( n = 3 per group). *p
    Figure Legend Snippet: Ibutilide treatment attenuates tunicamycin induced increase in Bax/ Bcl-2 ratio. Protein expression of Bax and Bcl-2 were analyzed from lysate derived from RNC treated with either tunicamycin alone (model), tunicamycin and ibutilide (treatment) or untreated cardiomyocytes (control). Representative immunoblots of lysate immunoblotted with antibodies specific for Bax or BCL-2 are shown. The quantification of normalized band densitometry of Bax was divided by that of Bcl-2 and the results are graphed. All data are shown as mean ± SE ( n = 3 per group). *p

    Techniques Used: Expressing, Derivative Assay, Western Blot

    4) Product Images from "Tumor necrosis factor-related apoptosis inducing ligand overexpression and Taxol treatment suppresses the growth of cervical cancer cells in vitro and in vivo"

    Article Title: Tumor necrosis factor-related apoptosis inducing ligand overexpression and Taxol treatment suppresses the growth of cervical cancer cells in vitro and in vivo

    Journal: Oncology Letters

    doi: 10.3892/ol.2018.8071

    Apoptosis of HeLa-vect and HeLa-TRAIL cells treated with Taxol. (A) AO/EB-stained cells. Scale bar, 100 µm. (B) Western blot analysis of cleaved caspase-3 and Bcl-2 protein expression. (C) Quantification of cleaved caspase-3 and Bcl-2 protein levels from three experiments. (D) DR5 mRNA levels in HeLa cells treated with different concentrations of Taxol. Data are from three separate experiments. Data are presented as the means ± SD. **P
    Figure Legend Snippet: Apoptosis of HeLa-vect and HeLa-TRAIL cells treated with Taxol. (A) AO/EB-stained cells. Scale bar, 100 µm. (B) Western blot analysis of cleaved caspase-3 and Bcl-2 protein expression. (C) Quantification of cleaved caspase-3 and Bcl-2 protein levels from three experiments. (D) DR5 mRNA levels in HeLa cells treated with different concentrations of Taxol. Data are from three separate experiments. Data are presented as the means ± SD. **P

    Techniques Used: Staining, Western Blot, Expressing

    H E, TUNEL, and immunohistochemical staining of HeLa tumor xenografts. (A, B) H E and TUNEL staining. (C, D) Immunohistochemical staining of cleaved caspase-3 (C) and Bcl-2 (D). Scale bar, 100 µm). (E) Quantification of the percentage of apoptotic cells in the TUNEL assay. (F) Quantification of cleaved caspase-3 and Bcl-2 protein expression detected by immunohistochemistry. Data are presented as the means ± SD. **P
    Figure Legend Snippet: H E, TUNEL, and immunohistochemical staining of HeLa tumor xenografts. (A, B) H E and TUNEL staining. (C, D) Immunohistochemical staining of cleaved caspase-3 (C) and Bcl-2 (D). Scale bar, 100 µm). (E) Quantification of the percentage of apoptotic cells in the TUNEL assay. (F) Quantification of cleaved caspase-3 and Bcl-2 protein expression detected by immunohistochemistry. Data are presented as the means ± SD. **P

    Techniques Used: TUNEL Assay, Immunohistochemistry, Staining, Expressing

    5) Product Images from "Croton Tiglium Extract Induces Apoptosis via Bax/Bcl-2 Pathways in Human Lung Cancer A549 Cells"

    Article Title: Croton Tiglium Extract Induces Apoptosis via Bax/Bcl-2 Pathways in Human Lung Cancer A549 Cells

    Journal: Asian Pacific Journal of Cancer Prevention : APJCP

    doi: 10.22034/APJCP.2016.17.11.4893

    Immunostaining for Apoptosis-Related Protein in A549 Cells Treated with Croton Tiglium Extract. (A) Representative immunofluorescence staining photomicrographs of Bax and Bcl-2 in A549 cells with 0 or 100 μg/ml Croton tiglium extract for 24 h, visualized by confocal microscope (×200). (B) Mean fluorescence activity of Bax and Bcl-2 expressions in A549 cells analyzed by image-pro plus 6.0 software. *: P
    Figure Legend Snippet: Immunostaining for Apoptosis-Related Protein in A549 Cells Treated with Croton Tiglium Extract. (A) Representative immunofluorescence staining photomicrographs of Bax and Bcl-2 in A549 cells with 0 or 100 μg/ml Croton tiglium extract for 24 h, visualized by confocal microscope (×200). (B) Mean fluorescence activity of Bax and Bcl-2 expressions in A549 cells analyzed by image-pro plus 6.0 software. *: P

    Techniques Used: Immunostaining, Immunofluorescence, Staining, Microscopy, Fluorescence, Activity Assay, Software

    6) Product Images from "Rhodiola rosea L. Attenuates Cigarette Smoke and Lipopolysaccharide-Induced COPD in Rats via Inflammation Inhibition and Antioxidant and Antifibrosis Pathways"

    Article Title: Rhodiola rosea L. Attenuates Cigarette Smoke and Lipopolysaccharide-Induced COPD in Rats via Inflammation Inhibition and Antioxidant and Antifibrosis Pathways

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    doi: 10.1155/2021/6103158

    The effect of RRL on ERK1/2 signaling, Smad3, and apoptosis of CS and LPS-induced COPD in rats. (a) Statistical analysis of Ras, p-Raf, and p-ERK1/2 protein expression levels. (b) Statistical analysis of Bax and Bcl-2 protein expression levels. (c) Statistical analysis of TGF- β 1 and Smad3 protein expression levels. Values are expressed as the mean ± SD ( n = 3); ## P
    Figure Legend Snippet: The effect of RRL on ERK1/2 signaling, Smad3, and apoptosis of CS and LPS-induced COPD in rats. (a) Statistical analysis of Ras, p-Raf, and p-ERK1/2 protein expression levels. (b) Statistical analysis of Bax and Bcl-2 protein expression levels. (c) Statistical analysis of TGF- β 1 and Smad3 protein expression levels. Values are expressed as the mean ± SD ( n = 3); ## P

    Techniques Used: Expressing

    7) Product Images from "Down-regulation of long non-coding RNA ESCCAL_1 inhibits tumor growth of esophageal squamous cell carcinoma in a xenograft mouse model"

    Article Title: Down-regulation of long non-coding RNA ESCCAL_1 inhibits tumor growth of esophageal squamous cell carcinoma in a xenograft mouse model

    Journal: Oncotarget

    doi: 10.18632/oncotarget.23153

    Diagram illustrates the underlying mechanism of ESCCAL_1 knockdown inhibits tumor growth in nude mice From the phenotype of overexpression of ESCCAL_1 promoting tumor growth to targets screening of phospho-kinase protein array, along with shRNA knockdown of ESCCAL_1 expression, the cross-talk of multiple signaling pathways may participate in ESCCAL_1-mediated oncogenesis. The inactivation of p-Src, p-FAK, p-JNK1, decrease of Bcl-2 expression and increase of p-p38, p53, BAX and Caspase-3 expression in ESCCAL_1 KD group were detected in this study (Red arrows: decrease; white arrows: increase; black arrows: activate/induce).
    Figure Legend Snippet: Diagram illustrates the underlying mechanism of ESCCAL_1 knockdown inhibits tumor growth in nude mice From the phenotype of overexpression of ESCCAL_1 promoting tumor growth to targets screening of phospho-kinase protein array, along with shRNA knockdown of ESCCAL_1 expression, the cross-talk of multiple signaling pathways may participate in ESCCAL_1-mediated oncogenesis. The inactivation of p-Src, p-FAK, p-JNK1, decrease of Bcl-2 expression and increase of p-p38, p53, BAX and Caspase-3 expression in ESCCAL_1 KD group were detected in this study (Red arrows: decrease; white arrows: increase; black arrows: activate/induce).

    Techniques Used: Mouse Assay, Over Expression, Protein Array, shRNA, Expressing

    Over-expression of ESCCAL_1 promotes survival and suppresses apoptosis ( A , C ) Protein levels of p-p38α, total p38α (t-p38α), p53, Bcl-2, BAX and Caspase-3 were detected by Western blot. ( B , D ) Quantitative protein levels of p-p38α, p53, Bcl-2, BAX and Caspase-3 were normalized with t-p38α or β-actin. Data are presented as mean ± SEM. n = 5 per group. * p
    Figure Legend Snippet: Over-expression of ESCCAL_1 promotes survival and suppresses apoptosis ( A , C ) Protein levels of p-p38α, total p38α (t-p38α), p53, Bcl-2, BAX and Caspase-3 were detected by Western blot. ( B , D ) Quantitative protein levels of p-p38α, p53, Bcl-2, BAX and Caspase-3 were normalized with t-p38α or β-actin. Data are presented as mean ± SEM. n = 5 per group. * p

    Techniques Used: Over Expression, Western Blot

    8) Product Images from "Effect of curcumin on Bcl-2 and Bax expression in nude mice prostate cancer"

    Article Title: Effect of curcumin on Bcl-2 and Bax expression in nude mice prostate cancer

    Journal: International Journal of Clinical and Experimental Pathology

    doi:

    Effect of curcumin on Bcl-2 and Bax expression in nude mice
    Figure Legend Snippet: Effect of curcumin on Bcl-2 and Bax expression in nude mice

    Techniques Used: Expressing, Mouse Assay

    The effect of curcumin on Bcl-2 expression in nude mice. Bcl-2 protein positively expressed in cytoplasm and cell membrane. Its expression decreased in curcumin group with dose dependence.
    Figure Legend Snippet: The effect of curcumin on Bcl-2 expression in nude mice. Bcl-2 protein positively expressed in cytoplasm and cell membrane. Its expression decreased in curcumin group with dose dependence.

    Techniques Used: Expressing, Mouse Assay

    Effect of curcumin on Bcl-2 and Bax expression in nude mice
    Figure Legend Snippet: Effect of curcumin on Bcl-2 and Bax expression in nude mice

    Techniques Used: Expressing, Mouse Assay

    9) Product Images from "Effect of curcumin on Bcl-2 and Bax expression in nude mice prostate cancer"

    Article Title: Effect of curcumin on Bcl-2 and Bax expression in nude mice prostate cancer

    Journal: International Journal of Clinical and Experimental Pathology

    doi:

    Effect of curcumin on Bcl-2 and Bax expression in nude mice
    Figure Legend Snippet: Effect of curcumin on Bcl-2 and Bax expression in nude mice

    Techniques Used: Expressing, Mouse Assay

    The effect of curcumin on Bcl-2 expression in nude mice. Bcl-2 protein positively expressed in cytoplasm and cell membrane. Its expression decreased in curcumin group with dose dependence.
    Figure Legend Snippet: The effect of curcumin on Bcl-2 expression in nude mice. Bcl-2 protein positively expressed in cytoplasm and cell membrane. Its expression decreased in curcumin group with dose dependence.

    Techniques Used: Expressing, Mouse Assay

    Effect of curcumin on Bcl-2 and Bax expression in nude mice
    Figure Legend Snippet: Effect of curcumin on Bcl-2 and Bax expression in nude mice

    Techniques Used: Expressing, Mouse Assay

    10) Product Images from "Exosomes Derived from miR-214-Enriched Bone Marrow-Derived Mesenchymal Stem Cells Regulate Oxidative Damage in Cardiac Stem Cells by Targeting CaMKII"

    Article Title: Exosomes Derived from miR-214-Enriched Bone Marrow-Derived Mesenchymal Stem Cells Regulate Oxidative Damage in Cardiac Stem Cells by Targeting CaMKII

    Journal: Oxidative Medicine and Cellular Longevity

    doi: 10.1155/2018/4971261

    Exosomes released from hypoxia-pretreated BMSCs protect CSCs from oxidative stress injury. CSCs cultured with 100 μ M H 2 O 2 were pretreated with BMSC-exos (400 μ g/ml) for 24 h and then subjected to analysis. (a) Representative dot plots of cell apoptosis after Annexin V/PI dual staining are shown. The left upper quadrant (% gated) shows necrotic cells (Annexin V−/PI+); the upper right quadrant (% gated) shows late apoptotic cells (Annexin V+/PI+); the left lower quadrant (% gated) shows live cells (Annexin V−/PI−); and the right lower quadrant (% gated) shows early apoptotic cells (Annexin V+/PI−). These cells were measured for comparison. (b) The percentage of apoptotic cells represents both early and late apoptotic cells. Compared with the H 2 O 2 -treated group, the BMSC-exo-treated group displayed a decreased percentage of apoptotic cells. In addition, Hypoxic-exos more markedly decreased apoptosis than did Nor-exos or Free-exos. (c) The intracellular ROS level was determined by FCM. The P2 percentage indicates the proportion of cells with increased ROS production, with signals above background 2′,7′-dichlorofluorescein (DCF) fluorescence levels. (d) Compared with the H 2 O 2 -treated group, the BMSC-exo-treated group had a significantly decreased intracellular ROS fluorescence intensity. In addition, Hypoxic-exos decreased ROS fluorescence to a greater degree than did Nor-exos or Free-exos. (e and f) The effects of BMSC-exos on cell apoptosis-related genes, such as procaspase-3, cleaved caspase-3, Bax, and Bcl-2 were detected by immunoblotting. Compared with the H 2 O 2 -treated cells, the BMSC-exo-treated cells had substantially decreased levels of cleaved caspase-3 and Bax and increased levels of Bcl-2. Additionally, Hypoxic-exos more markedly affected these protein levels than did Nor-exos. (g and h) Graph represents the SOD and MDA levels in CSCs; compared with H 2 O 2 group, Hypoxic-exos inhibited MDA levels and increased SOD production. (i) Representative immunofluorescence staining for TUNEL (green), C-kit (red), DAPI (blue), and merged images. Photos were randomly captured using a fluorescence microscope. Scale bar = 20 μ m. (j) The panel shows the percentage of TUNEL-positive cells. Compared with the H 2 O 2 -treated group, the BMSC-exo-treated group had significantly decreased percentage of TUNEL-positive cells. n = 3; ∗ P
    Figure Legend Snippet: Exosomes released from hypoxia-pretreated BMSCs protect CSCs from oxidative stress injury. CSCs cultured with 100 μ M H 2 O 2 were pretreated with BMSC-exos (400 μ g/ml) for 24 h and then subjected to analysis. (a) Representative dot plots of cell apoptosis after Annexin V/PI dual staining are shown. The left upper quadrant (% gated) shows necrotic cells (Annexin V−/PI+); the upper right quadrant (% gated) shows late apoptotic cells (Annexin V+/PI+); the left lower quadrant (% gated) shows live cells (Annexin V−/PI−); and the right lower quadrant (% gated) shows early apoptotic cells (Annexin V+/PI−). These cells were measured for comparison. (b) The percentage of apoptotic cells represents both early and late apoptotic cells. Compared with the H 2 O 2 -treated group, the BMSC-exo-treated group displayed a decreased percentage of apoptotic cells. In addition, Hypoxic-exos more markedly decreased apoptosis than did Nor-exos or Free-exos. (c) The intracellular ROS level was determined by FCM. The P2 percentage indicates the proportion of cells with increased ROS production, with signals above background 2′,7′-dichlorofluorescein (DCF) fluorescence levels. (d) Compared with the H 2 O 2 -treated group, the BMSC-exo-treated group had a significantly decreased intracellular ROS fluorescence intensity. In addition, Hypoxic-exos decreased ROS fluorescence to a greater degree than did Nor-exos or Free-exos. (e and f) The effects of BMSC-exos on cell apoptosis-related genes, such as procaspase-3, cleaved caspase-3, Bax, and Bcl-2 were detected by immunoblotting. Compared with the H 2 O 2 -treated cells, the BMSC-exo-treated cells had substantially decreased levels of cleaved caspase-3 and Bax and increased levels of Bcl-2. Additionally, Hypoxic-exos more markedly affected these protein levels than did Nor-exos. (g and h) Graph represents the SOD and MDA levels in CSCs; compared with H 2 O 2 group, Hypoxic-exos inhibited MDA levels and increased SOD production. (i) Representative immunofluorescence staining for TUNEL (green), C-kit (red), DAPI (blue), and merged images. Photos were randomly captured using a fluorescence microscope. Scale bar = 20 μ m. (j) The panel shows the percentage of TUNEL-positive cells. Compared with the H 2 O 2 -treated group, the BMSC-exo-treated group had significantly decreased percentage of TUNEL-positive cells. n = 3; ∗ P

    Techniques Used: Cell Culture, Staining, Fluorescence, Multiple Displacement Amplification, Immunofluorescence, TUNEL Assay, Microscopy

    Change in CaMKII expression during BMSC-exo-induced antioxidative injury in CSCs under oxidative stress. Cultured CSCs were transfected CaMKII3′ overexpression cDNA or siRCaMKII3′ for 48 h. Then, the cells were treated with BMSC-exos under different conditions for 24 h and/or cultured with 100 μ M H 2 O 2 for 2 h. (a) Representative immunofluorescence staining for TUNEL (green), C-kit (red), DAPI (blue), and merged images. Photos were randomly captured using a fluorescence microscope. Scale bar = 20 μ m. (b) The panel shows the percentages of TUNEL-positive cells. Compared with H 2 O 2 , SiRCaMKII3′ could significantly decreased the percentage of TUNEL-positive cells. Additionally, compared with Hypoxic-exos, CaMKII3′ could partially increase the percentage of TUNEL-positive cells. (c and d) The expression levels of procaspase-3, cleaved caspase-3, Bax, and Bcl-2 were detected by immunoblotting. Compared with Hypoxic-exos or SiRCaMKII3′ group, the CaMKII3′ group displayed substantially increased cleaved caspase-3 and Bax expression and decreased Bcl-2 expression. In addition, the Hypoxic-exo-induced protective effect against CSC apoptosis under oxidative stress was suppressed by CaMKII3′ overexpression. (e and f) Graph represents the SOD and MDA levels in CSCs; compared with H 2 O 2 group, Hypoxic-exos or SiRCaMKII3′ inhibited MDA levels and increased SOD production, while CSCs were transfected with CaMKII3′ increased MDA levels and suppressed SOD production. (g) Transient intracellular Ca 2+ measurement assays with Fluo-8/AM fluorescent labeling were used to detect Ca 2+ concentration in CSCs exposed to different treatments. (h) Compared with that in the H 2 O 2 or CaMKII3′ group, the fluorescence intensity of intracellular Ca 2+ was significantly decreased in the Hypoxic-exos or siRCaMKII group. Furthermore, CaMKII3′ overexpression could suppress the Hypoxic-exo-induced protective effect against CSC oxidative stress injury. n = 3; ∗ P
    Figure Legend Snippet: Change in CaMKII expression during BMSC-exo-induced antioxidative injury in CSCs under oxidative stress. Cultured CSCs were transfected CaMKII3′ overexpression cDNA or siRCaMKII3′ for 48 h. Then, the cells were treated with BMSC-exos under different conditions for 24 h and/or cultured with 100 μ M H 2 O 2 for 2 h. (a) Representative immunofluorescence staining for TUNEL (green), C-kit (red), DAPI (blue), and merged images. Photos were randomly captured using a fluorescence microscope. Scale bar = 20 μ m. (b) The panel shows the percentages of TUNEL-positive cells. Compared with H 2 O 2 , SiRCaMKII3′ could significantly decreased the percentage of TUNEL-positive cells. Additionally, compared with Hypoxic-exos, CaMKII3′ could partially increase the percentage of TUNEL-positive cells. (c and d) The expression levels of procaspase-3, cleaved caspase-3, Bax, and Bcl-2 were detected by immunoblotting. Compared with Hypoxic-exos or SiRCaMKII3′ group, the CaMKII3′ group displayed substantially increased cleaved caspase-3 and Bax expression and decreased Bcl-2 expression. In addition, the Hypoxic-exo-induced protective effect against CSC apoptosis under oxidative stress was suppressed by CaMKII3′ overexpression. (e and f) Graph represents the SOD and MDA levels in CSCs; compared with H 2 O 2 group, Hypoxic-exos or SiRCaMKII3′ inhibited MDA levels and increased SOD production, while CSCs were transfected with CaMKII3′ increased MDA levels and suppressed SOD production. (g) Transient intracellular Ca 2+ measurement assays with Fluo-8/AM fluorescent labeling were used to detect Ca 2+ concentration in CSCs exposed to different treatments. (h) Compared with that in the H 2 O 2 or CaMKII3′ group, the fluorescence intensity of intracellular Ca 2+ was significantly decreased in the Hypoxic-exos or siRCaMKII group. Furthermore, CaMKII3′ overexpression could suppress the Hypoxic-exo-induced protective effect against CSC oxidative stress injury. n = 3; ∗ P

    Techniques Used: Expressing, Cell Culture, Transfection, Over Expression, Immunofluorescence, Staining, TUNEL Assay, Fluorescence, Microscopy, Multiple Displacement Amplification, Labeling, Concentration Assay

    Exosomes derived from miR-214-modified BMSCs exert an antiapoptotic effect on CSCs under oxidative stress. BMSCs were transfected with miR-214 mimics, inhibitors, or negative control RNA. At 48 h posttransfection, exosomes were isolated from BMSCs pretreated with hypoxia and then added to CSCs under oxidative stress for 2 h. (a) RT-qPCR analysis of miR-214 expression in CSCs after different treatments. Compared with that in the H 2 O 2 group, miR-214 was significantly upregulated in mimic-exos group and substantially downregulated in the inhibitor-exos group. (b) RT-qPCR analysis of CaMKII mRNA expression in CSCs after different treatments. Compared with that in the H 2 O 2 group, CaMKII mRNA was significantly downregulated in the mimic-exos group but substantially upregulated in the inhibitor-exos group. (c and d) Western blotting was carried out to detect CaMKII protein levels, which revealed that compared with those in H 2 O 2 -treated CSCs, CaMKII levels were significantly downregulated in Hypoxic-exo- or mimic-exo-treated CSCs, whereas CaMKII protein levels were significantly upregulated in inhibitor-exo-treated CSCs. (e) Representative cell apoptosis dot plots after Annexin V/PI dual staining are shown. The upper left quadrant (% gated) shows necrotic cells (Annexin V−/PI+); the upper right quadrant (% gated) shows late apoptotic cells (Annexin V+/PI+); the left lower quadrant (% gated) shows live cells (Annexin V−/PI−); and the right lower quadrant (% gated) shows early apoptotic cells (Annexin V+/PI−). These cells were measured for comparison. (f) The percentage of apoptotic cells represents both early and late apoptotic cells. Compared with the H 2 O 2 group, the Hypoxic-exos or mimic-exos group displayed a decreased percentage of apoptotic cells. In addition, compared with Hypoxic-exos, inhibitor-exos increased the percentage of apoptotic cells. (g) Representative immunofluorescence staining for TUNEL (green), C-kit (red), DAPI (blue), and merged images. Photos were randomly captured using a fluorescence microscope. Scale bar = 20 μ m. (h) The panel shows the percentage of TUNEL-positive cells. Compared with H 2 O 2 , Hypoxic-exos or mimic-exos could significantly decrease the percentage of TUNEL-positive cells. Additionally, compared with Hypoxic-exos, inhibitor-exos could partially increase the percentage of TUNEL-positive cells. (i) The intr acellular ROS level was determined by FCM. The P2 percentage indicates the proportion of cells with increased ROS production, with signals above background DCF fluorescence levels. (j) Compared with that in H 2 O 2 -treated CSCs, the fluorescence intensity of intracellular ROS was decreased in Hypoxic-exo- or mimic-exo-treated CSCs. However, compared with Hypoxic-exos, inhibitor-exos decreased the fluorescence intensity of intracellular ROS in CSCs. (k and l) The expression levels of procaspase-3, cleaved caspase-3, Bax, and Bcl-2 were detected by immunoblotting. Compared with H 2 O 2 or inhibitor-exos, Hypoxic-exos or mimic-exos substantially decreased cleaved caspase-3 and Bax expression and increased Bcl-2 expression. (m and n) Graph represents the SOD and MDA levels in CSCs; compared with H 2 O 2 group, Hypoxic-exos or mimics-exos inhibited MDA levels and increased SOD production, while inhibitor-exos group increased MDA levels and suppressed SOD production. n = 3; ∗ P
    Figure Legend Snippet: Exosomes derived from miR-214-modified BMSCs exert an antiapoptotic effect on CSCs under oxidative stress. BMSCs were transfected with miR-214 mimics, inhibitors, or negative control RNA. At 48 h posttransfection, exosomes were isolated from BMSCs pretreated with hypoxia and then added to CSCs under oxidative stress for 2 h. (a) RT-qPCR analysis of miR-214 expression in CSCs after different treatments. Compared with that in the H 2 O 2 group, miR-214 was significantly upregulated in mimic-exos group and substantially downregulated in the inhibitor-exos group. (b) RT-qPCR analysis of CaMKII mRNA expression in CSCs after different treatments. Compared with that in the H 2 O 2 group, CaMKII mRNA was significantly downregulated in the mimic-exos group but substantially upregulated in the inhibitor-exos group. (c and d) Western blotting was carried out to detect CaMKII protein levels, which revealed that compared with those in H 2 O 2 -treated CSCs, CaMKII levels were significantly downregulated in Hypoxic-exo- or mimic-exo-treated CSCs, whereas CaMKII protein levels were significantly upregulated in inhibitor-exo-treated CSCs. (e) Representative cell apoptosis dot plots after Annexin V/PI dual staining are shown. The upper left quadrant (% gated) shows necrotic cells (Annexin V−/PI+); the upper right quadrant (% gated) shows late apoptotic cells (Annexin V+/PI+); the left lower quadrant (% gated) shows live cells (Annexin V−/PI−); and the right lower quadrant (% gated) shows early apoptotic cells (Annexin V+/PI−). These cells were measured for comparison. (f) The percentage of apoptotic cells represents both early and late apoptotic cells. Compared with the H 2 O 2 group, the Hypoxic-exos or mimic-exos group displayed a decreased percentage of apoptotic cells. In addition, compared with Hypoxic-exos, inhibitor-exos increased the percentage of apoptotic cells. (g) Representative immunofluorescence staining for TUNEL (green), C-kit (red), DAPI (blue), and merged images. Photos were randomly captured using a fluorescence microscope. Scale bar = 20 μ m. (h) The panel shows the percentage of TUNEL-positive cells. Compared with H 2 O 2 , Hypoxic-exos or mimic-exos could significantly decrease the percentage of TUNEL-positive cells. Additionally, compared with Hypoxic-exos, inhibitor-exos could partially increase the percentage of TUNEL-positive cells. (i) The intr acellular ROS level was determined by FCM. The P2 percentage indicates the proportion of cells with increased ROS production, with signals above background DCF fluorescence levels. (j) Compared with that in H 2 O 2 -treated CSCs, the fluorescence intensity of intracellular ROS was decreased in Hypoxic-exo- or mimic-exo-treated CSCs. However, compared with Hypoxic-exos, inhibitor-exos decreased the fluorescence intensity of intracellular ROS in CSCs. (k and l) The expression levels of procaspase-3, cleaved caspase-3, Bax, and Bcl-2 were detected by immunoblotting. Compared with H 2 O 2 or inhibitor-exos, Hypoxic-exos or mimic-exos substantially decreased cleaved caspase-3 and Bax expression and increased Bcl-2 expression. (m and n) Graph represents the SOD and MDA levels in CSCs; compared with H 2 O 2 group, Hypoxic-exos or mimics-exos inhibited MDA levels and increased SOD production, while inhibitor-exos group increased MDA levels and suppressed SOD production. n = 3; ∗ P

    Techniques Used: Derivative Assay, Modification, Transfection, Negative Control, Isolation, Quantitative RT-PCR, Expressing, Western Blot, Staining, Immunofluorescence, TUNEL Assay, Fluorescence, Microscopy, Multiple Displacement Amplification

    Effect of miR-214 expression on CSC apoptosis under oxidative stress. Cells were treated with miR-214 mimics, inhibitors, or negative control RNA for 48 h and/or pretreated with BMSC-exos (400 μ g/ml) for 24 h and then cultured with 100 μ M H 2 O 2 for 2 h for subsequent analyses. (a) RT-qPCR was used to analyze miR-214 expression in exosomes after normoxic or hypoxic preconditioning. Compared that in Nor-exos, miR-214 expression was significantly upregulated in Hypoxic-exos. (b) RT-qPCR analysis of miR-214 expression in CSCs after different treatments. Compared with that in the H 2 O 2 group, miR-214 significantly upregulated in the Hypoxic-exos or miR-214 mimics group. Compared with the Hypoxic-exos group, the inhibitor-exos group displayed a significantly decreased miR-214 expression. (c) Representative dot plots of cell apoptosis after Annexin V/PI dual staining are shown. The upper left quadrant (% gated) shows necrotic cells (Annexin V−/PI+); the upper right quadrant (% fated) shows late apoptotic cells (Annexin V+/PI+); the left lower quadrant (% gated) shows live cells (Annexin V−/PI−); and the right lower quadrant (% gated) shows early apoptotic cells (Annexin V+/PI−). These cells were measured for comparison. (d) The percentage of apoptotic cells represents both early and late apoptotic cells. Compared with H 2 O 2 or miR-214 inhibitor, Hypoxic-exos or miR-214 mimics decreased the percentage of apoptotic cells. In addition, the Hypoxic-exo-induced protective effect against CSC apoptosis under oxidative stress was partially suppressed by miR-214 inhibitors. (e) Representative immunofluorescence staining for TUNEL (green), C-kit (red), DAPI (blue), and merged images. Photos were randomly captured using a fluorescence microscope. Scale bar = 20 μ m. (f) The panel shows the percentage of TUNEL-positive cells. Compared with H 2 O 2 or miR-214 inhibitors, Hypoxic-exos or miR-214 mimics could significantly decrease the percentage of TUNEL-positive cells. In addition, compared with Hypoxic-exos, inhibitors-exos could partially increase the percentage of TUNEL-positive cells. (g) The intracellular ROS level was determined by FCM. The P2 percentage indicates the proportion of cells with increased ROS production, with signals above background DCF fluorescence levels. (h) Compared with that in CSCs treated with H 2 O 2 or miR-214 inhibitors, the fluorescence intensity of intracellular ROS was decreased in CSCs treated with Hypoxic-exos or miR-214 mimics. Inhibitor-exos showed higher ROS fluorescence intensity than Hypoxic-exos. (i and j) Graph represents the SOD and MDA levels in CSCs, compared with H 2 O 2 group, Hypoxic-exos or miR-214 mimics inhibited MDA levels and increased SOD production, while miR-214 inhibitors or inhibitor-exos increased MDA levels and suppressed SOD production. (k and l) The expression of apoptosis-related proteins, such as procaspase-3, cleaved caspase-3, Bax, and Bcl-2 were detected using immunoblotting. Compared with H 2 O 2 -treated cells, the cells treated with Hypoxic-exos or miR-214 mimics displayed substantially decreased cleaved caspase-3 and Bax expression and increased Bcl-2 expression. However, compared with Hypoxic-exos, miR-214 inhibitors or inhibitor-exos significantly increased cleaved caspase-3 and Bax expression but decreased Bcl-2 expression, n = 3; ∗ P
    Figure Legend Snippet: Effect of miR-214 expression on CSC apoptosis under oxidative stress. Cells were treated with miR-214 mimics, inhibitors, or negative control RNA for 48 h and/or pretreated with BMSC-exos (400 μ g/ml) for 24 h and then cultured with 100 μ M H 2 O 2 for 2 h for subsequent analyses. (a) RT-qPCR was used to analyze miR-214 expression in exosomes after normoxic or hypoxic preconditioning. Compared that in Nor-exos, miR-214 expression was significantly upregulated in Hypoxic-exos. (b) RT-qPCR analysis of miR-214 expression in CSCs after different treatments. Compared with that in the H 2 O 2 group, miR-214 significantly upregulated in the Hypoxic-exos or miR-214 mimics group. Compared with the Hypoxic-exos group, the inhibitor-exos group displayed a significantly decreased miR-214 expression. (c) Representative dot plots of cell apoptosis after Annexin V/PI dual staining are shown. The upper left quadrant (% gated) shows necrotic cells (Annexin V−/PI+); the upper right quadrant (% fated) shows late apoptotic cells (Annexin V+/PI+); the left lower quadrant (% gated) shows live cells (Annexin V−/PI−); and the right lower quadrant (% gated) shows early apoptotic cells (Annexin V+/PI−). These cells were measured for comparison. (d) The percentage of apoptotic cells represents both early and late apoptotic cells. Compared with H 2 O 2 or miR-214 inhibitor, Hypoxic-exos or miR-214 mimics decreased the percentage of apoptotic cells. In addition, the Hypoxic-exo-induced protective effect against CSC apoptosis under oxidative stress was partially suppressed by miR-214 inhibitors. (e) Representative immunofluorescence staining for TUNEL (green), C-kit (red), DAPI (blue), and merged images. Photos were randomly captured using a fluorescence microscope. Scale bar = 20 μ m. (f) The panel shows the percentage of TUNEL-positive cells. Compared with H 2 O 2 or miR-214 inhibitors, Hypoxic-exos or miR-214 mimics could significantly decrease the percentage of TUNEL-positive cells. In addition, compared with Hypoxic-exos, inhibitors-exos could partially increase the percentage of TUNEL-positive cells. (g) The intracellular ROS level was determined by FCM. The P2 percentage indicates the proportion of cells with increased ROS production, with signals above background DCF fluorescence levels. (h) Compared with that in CSCs treated with H 2 O 2 or miR-214 inhibitors, the fluorescence intensity of intracellular ROS was decreased in CSCs treated with Hypoxic-exos or miR-214 mimics. Inhibitor-exos showed higher ROS fluorescence intensity than Hypoxic-exos. (i and j) Graph represents the SOD and MDA levels in CSCs, compared with H 2 O 2 group, Hypoxic-exos or miR-214 mimics inhibited MDA levels and increased SOD production, while miR-214 inhibitors or inhibitor-exos increased MDA levels and suppressed SOD production. (k and l) The expression of apoptosis-related proteins, such as procaspase-3, cleaved caspase-3, Bax, and Bcl-2 were detected using immunoblotting. Compared with H 2 O 2 -treated cells, the cells treated with Hypoxic-exos or miR-214 mimics displayed substantially decreased cleaved caspase-3 and Bax expression and increased Bcl-2 expression. However, compared with Hypoxic-exos, miR-214 inhibitors or inhibitor-exos significantly increased cleaved caspase-3 and Bax expression but decreased Bcl-2 expression, n = 3; ∗ P

    Techniques Used: Expressing, Negative Control, Cell Culture, Quantitative RT-PCR, Staining, Immunofluorescence, TUNEL Assay, Fluorescence, Microscopy, Multiple Displacement Amplification

    11) Product Images from "Elevated expression of Dickkopf-1 increases the sensitivity of human glioma cell line SHG44 to BCNU"

    Article Title: Elevated expression of Dickkopf-1 increases the sensitivity of human glioma cell line SHG44 to BCNU

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    doi: 10.1186/1756-9966-29-131

    Bax, bcl-2 and caspase-3 protein expression inthree groups cell (×400) . (A) Bax normal SHG 44 ;(B)Bax SHG 44 -EV; (C)Bax SHG 44 -DKK-1;(D) Bcl-2 normal SHG 44 (E)Bcl-2 SHG 44 -EV; (F)Bcl-2 SHG 44 -DKK-1; (G)Caspase-3 normal SHG 44; (H)Caspase-3 SHG 44 -EV; (I)Caspase-3 SHG 44 -DKK-1
    Figure Legend Snippet: Bax, bcl-2 and caspase-3 protein expression inthree groups cell (×400) . (A) Bax normal SHG 44 ;(B)Bax SHG 44 -EV; (C)Bax SHG 44 -DKK-1;(D) Bcl-2 normal SHG 44 (E)Bcl-2 SHG 44 -EV; (F)Bcl-2 SHG 44 -DKK-1; (G)Caspase-3 normal SHG 44; (H)Caspase-3 SHG 44 -EV; (I)Caspase-3 SHG 44 -DKK-1

    Techniques Used: Expressing

    12) Product Images from "The protective effects of maltol on cisplatin-induced nephrotoxicity through the AMPK-mediated PI3K/Akt and p53 signaling pathways"

    Article Title: The protective effects of maltol on cisplatin-induced nephrotoxicity through the AMPK-mediated PI3K/Akt and p53 signaling pathways

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-34156-6

    Effects of maltol on the levels of inflammation cytokines in cisplatin-induced renal toxicity. ( A ) Effects of maltol on the positive expressions of Bax, Bcl-2, iNOS and COX-2 in renal tissues were examined by IHC in renal tissues (magnification × 200), And the column chart shows stained area, semiquantitative analysis of Bax, Bcl-2, iNOS and COX-2 expression in kidneys to IHC. ( B ) Inflammation cytokines level of TNF-α, IL-1β, iNOS and NF-κB in serum of mice were measured by ELISA kits. All values were expressed as mean ± S.D. * p
    Figure Legend Snippet: Effects of maltol on the levels of inflammation cytokines in cisplatin-induced renal toxicity. ( A ) Effects of maltol on the positive expressions of Bax, Bcl-2, iNOS and COX-2 in renal tissues were examined by IHC in renal tissues (magnification × 200), And the column chart shows stained area, semiquantitative analysis of Bax, Bcl-2, iNOS and COX-2 expression in kidneys to IHC. ( B ) Inflammation cytokines level of TNF-α, IL-1β, iNOS and NF-κB in serum of mice were measured by ELISA kits. All values were expressed as mean ± S.D. * p

    Techniques Used: Immunohistochemistry, Staining, Expressing, Mouse Assay, Enzyme-linked Immunosorbent Assay

    Protective effects of maltol on cisplatin-induced injury in HEK293 cells. ( A ) The cytotoxic effects of cisplatin on HEK293 cells. ( B ) Effect of maltol on the activity of normal cells. ( C ) The viability of HEK293 cells incubated with maltol after cisplatin exposure. Effects of maltol on the protein expression levels of Bcl-2, Bax and caspase 3, 8, 9 as well as GAPDH protein was used as a loading control. ( D ) Cells were used for western blot analysis of indicated proteins (upper panel). Column chart represents relative protein levels compared with the control group after normalization to GAPDH levels (lower panel) Values are expressed as mean ± S.D. n = 8. ** p
    Figure Legend Snippet: Protective effects of maltol on cisplatin-induced injury in HEK293 cells. ( A ) The cytotoxic effects of cisplatin on HEK293 cells. ( B ) Effect of maltol on the activity of normal cells. ( C ) The viability of HEK293 cells incubated with maltol after cisplatin exposure. Effects of maltol on the protein expression levels of Bcl-2, Bax and caspase 3, 8, 9 as well as GAPDH protein was used as a loading control. ( D ) Cells were used for western blot analysis of indicated proteins (upper panel). Column chart represents relative protein levels compared with the control group after normalization to GAPDH levels (lower panel) Values are expressed as mean ± S.D. n = 8. ** p

    Techniques Used: Activity Assay, Incubation, Expressing, Western Blot

    13) Product Images from "Agmatine protects Müller cells from high-concentration glucose-induced cell damage via N-methyl-D-aspartic acid receptor inhibition"

    Article Title: Agmatine protects Müller cells from high-concentration glucose-induced cell damage via N-methyl-D-aspartic acid receptor inhibition

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2015.3540

    Agmatine treatment may protect cells from glucose-induced apoptosis by increasing Bcl-2 (A), and decreasing Bax (B) and c-caspase-3 (C) expression levels. NMDA reversed the effects of agmatine. Data are presented as the mean ± standard deviation (n=3). ## P
    Figure Legend Snippet: Agmatine treatment may protect cells from glucose-induced apoptosis by increasing Bcl-2 (A), and decreasing Bax (B) and c-caspase-3 (C) expression levels. NMDA reversed the effects of agmatine. Data are presented as the mean ± standard deviation (n=3). ## P

    Techniques Used: Expressing, Standard Deviation

    14) Product Images from "In vitro and in vivo antitumor effects of acetylshikonin isolated from Arnebia euchroma (Royle) Johnst (Ruanzicao) cell suspension cultures"

    Article Title: In vitro and in vivo antitumor effects of acetylshikonin isolated from Arnebia euchroma (Royle) Johnst (Ruanzicao) cell suspension cultures

    Journal: Chinese Medicine

    doi: 10.1186/1749-8546-4-14

    Bax, bcl-2 and caspase-3 protein expression in LLC tissues (×400) . (A) Bax control; (B) Bax acetylshikonin (2 mg/kg); (C) Bcl-2 control; (D) Bcl-2 acetylshikonin (2 mg/kg); (E) caspase-3 control; (F) caspase-3 acetylshikonin (2 mg/kg).
    Figure Legend Snippet: Bax, bcl-2 and caspase-3 protein expression in LLC tissues (×400) . (A) Bax control; (B) Bax acetylshikonin (2 mg/kg); (C) Bcl-2 control; (D) Bcl-2 acetylshikonin (2 mg/kg); (E) caspase-3 control; (F) caspase-3 acetylshikonin (2 mg/kg).

    Techniques Used: Expressing

    15) Product Images from "Delivery of the co-expression plasmid pEndo-Si-Stat3 by attenuated Salmonella serovar typhimurium for prostate cancer treatment"

    Article Title: Delivery of the co-expression plasmid pEndo-Si-Stat3 by attenuated Salmonella serovar typhimurium for prostate cancer treatment

    Journal: Journal of cancer research and clinical oncology

    doi: 10.1007/s00432-013-1398-0

    Effects of treatment on the induction of apoptosis and Bcl-2 expression in tumors. a H E staining, immunohistochemical staining for Caspase3 (×400 mag.) and TUNEL staining (×400 mag.) were performed on tumor tissue obtained from
    Figure Legend Snippet: Effects of treatment on the induction of apoptosis and Bcl-2 expression in tumors. a H E staining, immunohistochemical staining for Caspase3 (×400 mag.) and TUNEL staining (×400 mag.) were performed on tumor tissue obtained from

    Techniques Used: Expressing, Staining, Immunohistochemistry, TUNEL Assay

    16) Product Images from "Tetrandrine Induces Mitochondria-Mediated Apoptosis in Human Gastric Cancer BGC-823 Cells"

    Article Title: Tetrandrine Induces Mitochondria-Mediated Apoptosis in Human Gastric Cancer BGC-823 Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0076486

    Immunohistochemical staining for apoptosis-related protein in BGC-823 xenografts. Immunohistochemical staining for apoptosis-related proteins: Bcl-2, Bcl-xl, Bax, Activated caspase-3, and Activated capase-9. Magnification is 200×. Representative microphotographs of three groups are shown. DAB stained immunoreactive cells (dark brown).
    Figure Legend Snippet: Immunohistochemical staining for apoptosis-related protein in BGC-823 xenografts. Immunohistochemical staining for apoptosis-related proteins: Bcl-2, Bcl-xl, Bax, Activated caspase-3, and Activated capase-9. Magnification is 200×. Representative microphotographs of three groups are shown. DAB stained immunoreactive cells (dark brown).

    Techniques Used: Immunohistochemistry, Staining

    The effect of tetrandrine on apoptosis-related proteins in BGC-823 cells. (A) Western blot analysis of the expression of apoptosis-related proteins in BGC-823 cells treated with 6, 8, and 10 μg/ml tetrandrine for 24 h. (B) Western blot analysis of bcl-2 and bax treated with 8 μg/ml tetrandrine for 12, 24, 48, and 72 h. (C) Effects of tetrandrine on the expression levels of bcl-2, bcl-xl, and bax were determined using real-time PCR (1), control (2) 6 μg/ml (3) 8 μg/ml (4) 10 μg/ml. β-actin expression was used as an internal control. Data are reported as the means ± SD of at least three experiments. * P
    Figure Legend Snippet: The effect of tetrandrine on apoptosis-related proteins in BGC-823 cells. (A) Western blot analysis of the expression of apoptosis-related proteins in BGC-823 cells treated with 6, 8, and 10 μg/ml tetrandrine for 24 h. (B) Western blot analysis of bcl-2 and bax treated with 8 μg/ml tetrandrine for 12, 24, 48, and 72 h. (C) Effects of tetrandrine on the expression levels of bcl-2, bcl-xl, and bax were determined using real-time PCR (1), control (2) 6 μg/ml (3) 8 μg/ml (4) 10 μg/ml. β-actin expression was used as an internal control. Data are reported as the means ± SD of at least three experiments. * P

    Techniques Used: Western Blot, Expressing, Real-time Polymerase Chain Reaction

    17) Product Images from "The Toxicity Mechanisms of Action of Aβ25–35 in Isolated Rat Cardiac Myocytes"

    Article Title: The Toxicity Mechanisms of Action of Aβ25–35 in Isolated Rat Cardiac Myocytes

    Journal: Molecules

    doi: 10.3390/molecules190812242

    ( A ) Aβ 25–35 induced the expression of the apoptosis protein in the cardiac myocyte; ( B ) Aβ 25–35 induced the expression of ER stress marker in the cardiac myocyte. Cells were treated with indicated concentrations of Aβ 25–35 for 24 h and subjected to western blotting to measure the related protein; ( C – D ) Relative protein levels were quantified by densitometry and shown in the histogram; ( E ) The ratio of the Bax/Bcl-2; ( F ) Aβ 25–35 decreased the expression ROCK protein. Values are expressed as mean ± SEM of three independent experiments, each in triplicate. * p
    Figure Legend Snippet: ( A ) Aβ 25–35 induced the expression of the apoptosis protein in the cardiac myocyte; ( B ) Aβ 25–35 induced the expression of ER stress marker in the cardiac myocyte. Cells were treated with indicated concentrations of Aβ 25–35 for 24 h and subjected to western blotting to measure the related protein; ( C – D ) Relative protein levels were quantified by densitometry and shown in the histogram; ( E ) The ratio of the Bax/Bcl-2; ( F ) Aβ 25–35 decreased the expression ROCK protein. Values are expressed as mean ± SEM of three independent experiments, each in triplicate. * p

    Techniques Used: Expressing, Marker, Western Blot

    18) Product Images from "Expression of TP53, BCL-2, and VEGFA Genes in Esophagus Carcinoma and its Biological Significance"

    Article Title: Expression of TP53, BCL-2, and VEGFA Genes in Esophagus Carcinoma and its Biological Significance

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    doi: 10.12659/MSM.894640

    Expression of Bcl-2 proteins by IHC staining. ( A ) Control group: ( B ) Model group at 4 weeks; ( C ) Model group at 10 weeks; ( D ) Model group at 20 weeks; ( E ) Model group at 30 weeks. Magnification, ×200.
    Figure Legend Snippet: Expression of Bcl-2 proteins by IHC staining. ( A ) Control group: ( B ) Model group at 4 weeks; ( C ) Model group at 10 weeks; ( D ) Model group at 20 weeks; ( E ) Model group at 30 weeks. Magnification, ×200.

    Techniques Used: Expressing, Immunohistochemistry, Staining

    19) Product Images from "GSK-J4 induces cell cycle arrest and apoptosis via ER stress and the synergism between GSK-J4 and decitabine in acute myeloid leukemia KG-1a cells"

    Article Title: GSK-J4 induces cell cycle arrest and apoptosis via ER stress and the synergism between GSK-J4 and decitabine in acute myeloid leukemia KG-1a cells

    Journal: Cancer Cell International

    doi: 10.1186/s12935-020-01297-6

    The mechanisms of GSK-J4-induced cell cycle arrest, cell apoptosis and PKC-α/p-Bcl2 pathway inhibition. GSK-J4 induces S phase arrest and cell apoptosis through ER stress, and inhibits PKC-α/p-Bcl2 pathway by stimulating ER stress
    Figure Legend Snippet: The mechanisms of GSK-J4-induced cell cycle arrest, cell apoptosis and PKC-α/p-Bcl2 pathway inhibition. GSK-J4 induces S phase arrest and cell apoptosis through ER stress, and inhibits PKC-α/p-Bcl2 pathway by stimulating ER stress

    Techniques Used: Inhibition

    The role of ER stress in GSK-J4-regulated PKC-α/p-Bcl2 pathway inhibition. a After treatment with PKC-α siRNA NC, siRNA1, siRNA2 and siRNA3, the expression level of PKC-α was detected by Western blotting. b After treated with siRNA2, the ratio of apoptotic cells was detected using flow cytometry. c Statistical analysis of the apoptotic rate of KG-1a treated with siRNA2. Values represent the mean ± SD of three independent experiments. * p
    Figure Legend Snippet: The role of ER stress in GSK-J4-regulated PKC-α/p-Bcl2 pathway inhibition. a After treatment with PKC-α siRNA NC, siRNA1, siRNA2 and siRNA3, the expression level of PKC-α was detected by Western blotting. b After treated with siRNA2, the ratio of apoptotic cells was detected using flow cytometry. c Statistical analysis of the apoptotic rate of KG-1a treated with siRNA2. Values represent the mean ± SD of three independent experiments. * p

    Techniques Used: Inhibition, Expressing, Western Blot, Flow Cytometry

    20) Product Images from "Antitumor Effects of Laminaria Extract Fucoxanthin on Lung Cancer"

    Article Title: Antitumor Effects of Laminaria Extract Fucoxanthin on Lung Cancer

    Journal: Marine Drugs

    doi: 10.3390/md15020039

    FX induced G 0 /G1 arrest and apoptosis through regulating p21 waf1/cip1 , p53, Bcl-2, PUMA and Fas. Total cell RNA was extracted from A549 ( A ) and H1299 ( B ) cells after treating with FX (0, 12.5, 25, 50, 75 and 100 μM) for 48 h. Corresponding mRNA levels of p53, p21 waf1/cip1 , Bcl-2, PUMA and Fas genes were determined using qRT-PCR. Results were shown as the relative expression ratio of genes in A549 and H1299 cells, respectively. Western blot analysis of cell cycle arrest and apoptosis related proteins: p53, P-p53, PUMA, p21 waf1/cip1 , Fas and Bcl-2, were performed after A549 ( C ) and H1299 ( D ) cells harvested after exposing to various concentrations of FX (0, 12.5, 25, 50 μM). Effects of FX on caspase-3/8 activities in A549 ( E ) and H1299 ( F ) cells were measured after 48 h of exposure similar to panel C/D by colorimetric method. When presented, means and standard deviations were obtained from three independent experiments. * p
    Figure Legend Snippet: FX induced G 0 /G1 arrest and apoptosis through regulating p21 waf1/cip1 , p53, Bcl-2, PUMA and Fas. Total cell RNA was extracted from A549 ( A ) and H1299 ( B ) cells after treating with FX (0, 12.5, 25, 50, 75 and 100 μM) for 48 h. Corresponding mRNA levels of p53, p21 waf1/cip1 , Bcl-2, PUMA and Fas genes were determined using qRT-PCR. Results were shown as the relative expression ratio of genes in A549 and H1299 cells, respectively. Western blot analysis of cell cycle arrest and apoptosis related proteins: p53, P-p53, PUMA, p21 waf1/cip1 , Fas and Bcl-2, were performed after A549 ( C ) and H1299 ( D ) cells harvested after exposing to various concentrations of FX (0, 12.5, 25, 50 μM). Effects of FX on caspase-3/8 activities in A549 ( E ) and H1299 ( F ) cells were measured after 48 h of exposure similar to panel C/D by colorimetric method. When presented, means and standard deviations were obtained from three independent experiments. * p

    Techniques Used: Quantitative RT-PCR, Expressing, Western Blot

    Effects of FX on the protein expression of Bcl-2 and Caspase-3 and apoptosis rates in A549 xenograft tumors. ( A ) TUNEL staining; Bcl-2 ( B ); and Caspase-3 ( C ) immunohistochemical staining. Magnification: 400×. FX 5, FX 15, FX 50 mean 5, 15 and 50 mg/kg FX respectively. Data are expressed as means ± SD ( n = 5). * p
    Figure Legend Snippet: Effects of FX on the protein expression of Bcl-2 and Caspase-3 and apoptosis rates in A549 xenograft tumors. ( A ) TUNEL staining; Bcl-2 ( B ); and Caspase-3 ( C ) immunohistochemical staining. Magnification: 400×. FX 5, FX 15, FX 50 mean 5, 15 and 50 mg/kg FX respectively. Data are expressed as means ± SD ( n = 5). * p

    Techniques Used: Expressing, TUNEL Assay, Staining, Immunohistochemistry

    21) Product Images from "Hepatitis E Virus Induces Hepatocyte Apoptosis via Mitochondrial Pathway in Mongolian Gerbils"

    Article Title: Hepatitis E Virus Induces Hepatocyte Apoptosis via Mitochondrial Pathway in Mongolian Gerbils

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2018.00460

    Immunohistochemical and western-blot analysis of Bax, Bcl-2, and cytochrome c proteins. (A1) IHC analysis of livers in control group, (A2) IHC analysis of livers in inoculated group. The primary antibody was Bax antibody, and positive signals were observed in the cytoplasm of hepatocyte (→); (A3) A semi-quantitative analysis of the ratio of Bax positive staining to the total field; (A4) Western-blot analysis of Bax in hepatocyte of control group and inoculated group gerbils; (A5) A semi-quantitative analysis of relative expression of Bax/β-actin. (B1) IHC analysis of livers in control group, (B2) IHC analysis of livers in inoculated group. The primary antibody was Bcl-2 antibody, and the positive signals were observed in the cytoplasm of hepatocyte (→); (B3) A semi-quantitative analysis of the ratio of Bcl-2 positive staining to the total field; (B4) Western-blot analysis of Bcl-2 in hepatocyte of control group and inoculated group gerbils; (B5) A semi-quantitative analysis of relative expression of Bcl-2/β-actin. (C1) Western-blot analysis of cytochrome c in mitochondrion and cytoplasm of hepatocyte of control group and inoculated group gerbils. (C2) A semi-quantitative analysis of relative expression of cytochrome c in mitochondria/β-actin. (C3) A semi-quantitative analysis of relative expression of cytochrome c in cytoplasm/β-actin. ∗ p
    Figure Legend Snippet: Immunohistochemical and western-blot analysis of Bax, Bcl-2, and cytochrome c proteins. (A1) IHC analysis of livers in control group, (A2) IHC analysis of livers in inoculated group. The primary antibody was Bax antibody, and positive signals were observed in the cytoplasm of hepatocyte (→); (A3) A semi-quantitative analysis of the ratio of Bax positive staining to the total field; (A4) Western-blot analysis of Bax in hepatocyte of control group and inoculated group gerbils; (A5) A semi-quantitative analysis of relative expression of Bax/β-actin. (B1) IHC analysis of livers in control group, (B2) IHC analysis of livers in inoculated group. The primary antibody was Bcl-2 antibody, and the positive signals were observed in the cytoplasm of hepatocyte (→); (B3) A semi-quantitative analysis of the ratio of Bcl-2 positive staining to the total field; (B4) Western-blot analysis of Bcl-2 in hepatocyte of control group and inoculated group gerbils; (B5) A semi-quantitative analysis of relative expression of Bcl-2/β-actin. (C1) Western-blot analysis of cytochrome c in mitochondrion and cytoplasm of hepatocyte of control group and inoculated group gerbils. (C2) A semi-quantitative analysis of relative expression of cytochrome c in mitochondria/β-actin. (C3) A semi-quantitative analysis of relative expression of cytochrome c in cytoplasm/β-actin. ∗ p

    Techniques Used: Immunohistochemistry, Western Blot, Staining, Expressing

    22) Product Images from "The Effects of Fasudil at Different Doses on Acute Myocardial Infarction in Rats"

    Article Title: The Effects of Fasudil at Different Doses on Acute Myocardial Infarction in Rats

    Journal: Acta Cardiologica Sinica

    doi:

    Expression of Bcl-2 in immunohistochemistry staining: compared with E, the expression of Bcl-2 in A, B, C and D is decreased; but as fasudil injection doses increased, the expression of Bax is gradually increased.
    Figure Legend Snippet: Expression of Bcl-2 in immunohistochemistry staining: compared with E, the expression of Bcl-2 in A, B, C and D is decreased; but as fasudil injection doses increased, the expression of Bax is gradually increased.

    Techniques Used: Expressing, Immunohistochemistry, Staining, Injection

    Expression changes of Rho kinase mRNA and Bcl-2 and Bax in myocardial cells
    Figure Legend Snippet: Expression changes of Rho kinase mRNA and Bcl-2 and Bax in myocardial cells

    Techniques Used: Expressing

    23) Product Images from "Anti-tumor effects of Astragalus on hepatocellular carcinoma in vivo"

    Article Title: Anti-tumor effects of Astragalus on hepatocellular carcinoma in vivo

    Journal: Indian Journal of Pharmacology

    doi: 10.4103/0253-7613.91872

    Expression of Bax and Bcl-2 proteins in transplanted tumor cells of H22 bearing mice (a) Representative immunostaining for Bax proteins in H22 bearing mice (×200. (b) Representative immunostaining for Bcl-2 proteins in H22 bearing mice (×200. (c) Semi-quantitative analysis of the optical density of Bax. (d) Semi-quantitative analysis of the optical density of of Bcl-2. (e) The calculated Bax/Bcl-2 ratio. Values are shown as mean±SD (n=10). * P
    Figure Legend Snippet: Expression of Bax and Bcl-2 proteins in transplanted tumor cells of H22 bearing mice (a) Representative immunostaining for Bax proteins in H22 bearing mice (×200. (b) Representative immunostaining for Bcl-2 proteins in H22 bearing mice (×200. (c) Semi-quantitative analysis of the optical density of Bax. (d) Semi-quantitative analysis of the optical density of of Bcl-2. (e) The calculated Bax/Bcl-2 ratio. Values are shown as mean±SD (n=10). * P

    Techniques Used: Expressing, Mouse Assay, Immunostaining

    24) Product Images from "Effects of rosmarinic acid on immunoregulatory activity and hepatocellular carcinoma cell apoptosis in H22 tumor-bearing mice"

    Article Title: Effects of rosmarinic acid on immunoregulatory activity and hepatocellular carcinoma cell apoptosis in H22 tumor-bearing mice

    Journal: The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology

    doi: 10.4196/kjpp.2019.23.6.501

    Effects of rosmarinic acid (RA) on expressions of Bax , Bcl-2 , and Caspase-3 in xenograft tumor tissues. Expressions of Bax , Bcl-2 , and Caspase-3 proteins were measured by immunohistochemistry (magnification, ×400). The images were analyzed by an Image Pro Plus 6.0 system. Expressions of Bax , Bcl-2 , and Caspase-3 mRNA were measured by qRT-PCR. GAPDH was used as a loading control. (A, D) Bax protein expression; (B, E) Bcl-2 protein expression; (C, F) Caspase-3 protein expression. (G) Bax mRNA expression; (H) Bcl-2 mRNA expression; (I) Caspase-3 mRNA expression. Data were presented as mean ± standard deviation. IOD, integrated opticdensity. * p
    Figure Legend Snippet: Effects of rosmarinic acid (RA) on expressions of Bax , Bcl-2 , and Caspase-3 in xenograft tumor tissues. Expressions of Bax , Bcl-2 , and Caspase-3 proteins were measured by immunohistochemistry (magnification, ×400). The images were analyzed by an Image Pro Plus 6.0 system. Expressions of Bax , Bcl-2 , and Caspase-3 mRNA were measured by qRT-PCR. GAPDH was used as a loading control. (A, D) Bax protein expression; (B, E) Bcl-2 protein expression; (C, F) Caspase-3 protein expression. (G) Bax mRNA expression; (H) Bcl-2 mRNA expression; (I) Caspase-3 mRNA expression. Data were presented as mean ± standard deviation. IOD, integrated opticdensity. * p

    Techniques Used: Immunohistochemistry, Quantitative RT-PCR, Expressing, Standard Deviation

    25) Product Images from "Comparative Study on the Protective Effects of Salidroside and Hypoxic Preconditioning for Attenuating Anoxia-Induced Apoptosis in Pheochromocytoma (PC12) Cells"

    Article Title: Comparative Study on the Protective Effects of Salidroside and Hypoxic Preconditioning for Attenuating Anoxia-Induced Apoptosis in Pheochromocytoma (PC12) Cells

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    doi: 10.12659/MSM.897640

    Effects of salidroside on expression of apoptotic signaling proteins Bcl-2 of PC12 cells in different incubation conditions. ( A ) BcL-2 protein was analyzed by immunoblot; ( B ) The relative quantification of Bcl-2 in each group by corresponding internal control and the intensity of the band of control group. (* P
    Figure Legend Snippet: Effects of salidroside on expression of apoptotic signaling proteins Bcl-2 of PC12 cells in different incubation conditions. ( A ) BcL-2 protein was analyzed by immunoblot; ( B ) The relative quantification of Bcl-2 in each group by corresponding internal control and the intensity of the band of control group. (* P

    Techniques Used: Expressing, Incubation

    26) Product Images from "Knockdown of High Mobility Group-Box 3 (HMGB3) Expression Inhibits Proliferation, Reduces Migration, and Affects Chemosensitivity in Gastric Cancer Cells"

    Article Title: Knockdown of High Mobility Group-Box 3 (HMGB3) Expression Inhibits Proliferation, Reduces Migration, and Affects Chemosensitivity in Gastric Cancer Cells

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    doi: 10.12659/MSM.900880

    ( A, B ) Protein expression levels of p21, p53, Bax, and Bcl-2 were detected by Western blot assays and compared by quantitative analysis of the gray value. * p
    Figure Legend Snippet: ( A, B ) Protein expression levels of p21, p53, Bax, and Bcl-2 were detected by Western blot assays and compared by quantitative analysis of the gray value. * p

    Techniques Used: Expressing, Western Blot

    27) Product Images from "The Viral Oncoprotein HBx of Hepatitis B virus Promotes the Growth of Hepatocellular Carcinoma through Cooperating with the Cellular Oncoprotein RMP"

    Article Title: The Viral Oncoprotein HBx of Hepatitis B virus Promotes the Growth of Hepatocellular Carcinoma through Cooperating with the Cellular Oncoprotein RMP

    Journal: International Journal of Biological Sciences

    doi: 10.7150/ijbs.10275

    HBx and RMP collaborately regulate apoptotic genes. (A). HepG2 cells (lane 1) were transfected with empty vector (lane 2), expression vectors for RMP (lane 3), HBx (lane 4), or both RMP and HBx (lane 5). Protein was extracted and Western blot analysis was carried out with antibodies against Bax, Bcl-2 and GAPDH which was applied for the normalization. The relative expression of Bax and Bcl-2 against GAPDH were graphically depicted in (B). (C). HepG2 cells were transfected with vectors as indicated. The mRNA was extracted and quantative RT-PCR was carried out for the determination of expression of p53. The expression of GAPDH was also determined for the normalization of expression of above factors. Student's t test, *, p
    Figure Legend Snippet: HBx and RMP collaborately regulate apoptotic genes. (A). HepG2 cells (lane 1) were transfected with empty vector (lane 2), expression vectors for RMP (lane 3), HBx (lane 4), or both RMP and HBx (lane 5). Protein was extracted and Western blot analysis was carried out with antibodies against Bax, Bcl-2 and GAPDH which was applied for the normalization. The relative expression of Bax and Bcl-2 against GAPDH were graphically depicted in (B). (C). HepG2 cells were transfected with vectors as indicated. The mRNA was extracted and quantative RT-PCR was carried out for the determination of expression of p53. The expression of GAPDH was also determined for the normalization of expression of above factors. Student's t test, *, p

    Techniques Used: Transfection, Plasmid Preparation, Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction

    28) Product Images from "miR-21 Reduces Hydrogen Peroxide-Induced Apoptosis in c-kit+ Cardiac Stem Cells In Vitro through PTEN/PI3K/Akt Signaling"

    Article Title: miR-21 Reduces Hydrogen Peroxide-Induced Apoptosis in c-kit+ Cardiac Stem Cells In Vitro through PTEN/PI3K/Akt Signaling

    Journal: Oxidative Medicine and Cellular Longevity

    doi: 10.1155/2016/5389181

    miR-21's effect on apoptosis related proteins in CSCs. Cultured CSCs were treated with miR-21 mimics, inhibitor, or scrambles for 48 h or challenged by 100 μ M H 2 O 2 for 2 h at the same time. (a) c-kit + CSCs were triple stained by c-kit (green), caspase (orange), and DAPI (blue) and observed under a fluorescence microscope (Olympus, Japan). Bar = 50 μ m. PI: propidium iodide. (b) miR-21 mimics' influences on Bax, Bcl-2, and caspase-3 detected with immune blotting. (c) Statistics of the relative expression level of proteins in (b). miR-21 mimics increased the expression of Bcl-2 and decreased Bax and caspase-3 compared with H 2 O 2 . ∗ P
    Figure Legend Snippet: miR-21's effect on apoptosis related proteins in CSCs. Cultured CSCs were treated with miR-21 mimics, inhibitor, or scrambles for 48 h or challenged by 100 μ M H 2 O 2 for 2 h at the same time. (a) c-kit + CSCs were triple stained by c-kit (green), caspase (orange), and DAPI (blue) and observed under a fluorescence microscope (Olympus, Japan). Bar = 50 μ m. PI: propidium iodide. (b) miR-21 mimics' influences on Bax, Bcl-2, and caspase-3 detected with immune blotting. (c) Statistics of the relative expression level of proteins in (b). miR-21 mimics increased the expression of Bcl-2 and decreased Bax and caspase-3 compared with H 2 O 2 . ∗ P

    Techniques Used: Cell Culture, Staining, Fluorescence, Microscopy, Expressing

    29) Product Images from "Ginsenoside Rk1 ameliorates paracetamol-induced hepatotoxicity in mice through inhibition of inflammation, oxidative stress, nitrative stress and apoptosis"

    Article Title: Ginsenoside Rk1 ameliorates paracetamol-induced hepatotoxicity in mice through inhibition of inflammation, oxidative stress, nitrative stress and apoptosis

    Journal: Journal of Ginseng Research

    doi: 10.1016/j.jgr.2017.07.003

    Protein expression of Bax and Bcl-2. (A) Effects of Rk1 on the protein expression of Bax and Bcl-2. (B) Results quantified from Bax and Bcl-2 band intensities. The protein expression was examined using western blotting analysis in liver tissues. All data are expressed as the mean ± standard deviation, n = 8. * p
    Figure Legend Snippet: Protein expression of Bax and Bcl-2. (A) Effects of Rk1 on the protein expression of Bax and Bcl-2. (B) Results quantified from Bax and Bcl-2 band intensities. The protein expression was examined using western blotting analysis in liver tissues. All data are expressed as the mean ± standard deviation, n = 8. * p

    Techniques Used: Expressing, Western Blot, Standard Deviation

    Pretreatment with Rk1 protected against APAP-induced liver of Rk1 on the expression of: (A, E) nitric oxide synthase (iNOS), (B, F) cyclooxygenase-2 (COX-2), (C, G) Bax, and (D, H) Bcl-2. The protein expression was examined by immunohistochemistry. All data are expressed as the mean ± standard deviation, n = 8. ** p
    Figure Legend Snippet: Pretreatment with Rk1 protected against APAP-induced liver of Rk1 on the expression of: (A, E) nitric oxide synthase (iNOS), (B, F) cyclooxygenase-2 (COX-2), (C, G) Bax, and (D, H) Bcl-2. The protein expression was examined by immunohistochemistry. All data are expressed as the mean ± standard deviation, n = 8. ** p

    Techniques Used: Expressing, Immunohistochemistry, Standard Deviation

    30) Product Images from "Inhibition by Multifunctional Magnetic Nanoparticles Loaded with Alpha-Synuclein RNAi Plasmid in a Parkinson's Disease Model"

    Article Title: Inhibition by Multifunctional Magnetic Nanoparticles Loaded with Alpha-Synuclein RNAi Plasmid in a Parkinson's Disease Model

    Journal: Theranostics

    doi: 10.7150/thno.16562

    Measured efficacy data in vitro . (a), Cellular apoptosis model induced by MPP + at 24 h, 72 h and 144 h, and pDNA concentration screening. (b), The LDH expression determined using the LDH detection kit for the control, NP-NIPAm-AA-NGF and NP-NIPAm-AA-NGF (pDNA) group at 72 h and 144 h. (c), Cell morphology evaluated by light microscopy (black bars, 10 μm). (d), Cell mortality data assessed by flow cytometry in the three groups: control, NP-NIPAm-AA-NGF, and NP-NIPAm-AA-NGF (pDNA) at 72 h and 144 h. (e), Protein expression of α-syn by western blot analysis in the control, NP-NIPAm-AA-NGF, NP-NIPAm-AA-NGF (pDNA) and NP-NIPAm-AA-NGF (empty pDNA) groups at 72 h and 144 h. (f), Protein expression of NGFR, Bax, Bcl-2, and P53 determined by western blot analysis in the control, NP-NIPAm-AA-NGF and NP-NIPAm-AA-NGF (pDNA) groups at 72 h and 144 h. The blots were re-probed to detect β-actin as a control to confirm equal protein loading. Protein expression determined using Image pro-plus 6.0. (g). The possible molecular mechanisms for multifunctional nano-biomaterials release α-syn interference plasmid to inhibit the synthesis of α-syn. The relative levels are plotted at a significance of p
    Figure Legend Snippet: Measured efficacy data in vitro . (a), Cellular apoptosis model induced by MPP + at 24 h, 72 h and 144 h, and pDNA concentration screening. (b), The LDH expression determined using the LDH detection kit for the control, NP-NIPAm-AA-NGF and NP-NIPAm-AA-NGF (pDNA) group at 72 h and 144 h. (c), Cell morphology evaluated by light microscopy (black bars, 10 μm). (d), Cell mortality data assessed by flow cytometry in the three groups: control, NP-NIPAm-AA-NGF, and NP-NIPAm-AA-NGF (pDNA) at 72 h and 144 h. (e), Protein expression of α-syn by western blot analysis in the control, NP-NIPAm-AA-NGF, NP-NIPAm-AA-NGF (pDNA) and NP-NIPAm-AA-NGF (empty pDNA) groups at 72 h and 144 h. (f), Protein expression of NGFR, Bax, Bcl-2, and P53 determined by western blot analysis in the control, NP-NIPAm-AA-NGF and NP-NIPAm-AA-NGF (pDNA) groups at 72 h and 144 h. The blots were re-probed to detect β-actin as a control to confirm equal protein loading. Protein expression determined using Image pro-plus 6.0. (g). The possible molecular mechanisms for multifunctional nano-biomaterials release α-syn interference plasmid to inhibit the synthesis of α-syn. The relative levels are plotted at a significance of p

    Techniques Used: In Vitro, Concentration Assay, Expressing, Light Microscopy, Flow Cytometry, Cytometry, Western Blot, Plasmid Preparation

    31) Product Images from "Hydroxy-α-sanshool Possesses Protective Potentials on H2O2-Stimulated PC12 Cells by Suppression of Oxidative Stress-Induced Apoptosis through Regulation of PI3K/Akt Signal Pathway"

    Article Title: Hydroxy-α-sanshool Possesses Protective Potentials on H2O2-Stimulated PC12 Cells by Suppression of Oxidative Stress-Induced Apoptosis through Regulation of PI3K/Akt Signal Pathway

    Journal: Oxidative Medicine and Cellular Longevity

    doi: 10.1155/2020/3481758

    Effects of HAS on protein expressions of caspase-3, Bax, Bcl-2, PI3K, p-PI3K, Akt, and p-Akt in H 2 O 2 -stimulated PC12 cells. PC12 cells were treated with HAS (15, 30, and 60 μ M) or 100 μ M vitamin C for 2 h, subsequently subjected to H 2 O 2 (90 μ M) for 4 h. Protein expressions of caspase-3, Bax, Bcl-2, PI3K, p-PI3K, Akt, and p-Akt were determined by western blotting, and β -actin was used as the internal reference. The values were represented as the mean ± SD ( n = 3). ∗∗ P
    Figure Legend Snippet: Effects of HAS on protein expressions of caspase-3, Bax, Bcl-2, PI3K, p-PI3K, Akt, and p-Akt in H 2 O 2 -stimulated PC12 cells. PC12 cells were treated with HAS (15, 30, and 60 μ M) or 100 μ M vitamin C for 2 h, subsequently subjected to H 2 O 2 (90 μ M) for 4 h. Protein expressions of caspase-3, Bax, Bcl-2, PI3K, p-PI3K, Akt, and p-Akt were determined by western blotting, and β -actin was used as the internal reference. The values were represented as the mean ± SD ( n = 3). ∗∗ P

    Techniques Used: Western Blot

    32) Product Images from "Ibutilide protects against cardiomyocytes injury via inhibiting endoplasmic reticulum and mitochondrial stress pathways"

    Article Title: Ibutilide protects against cardiomyocytes injury via inhibiting endoplasmic reticulum and mitochondrial stress pathways

    Journal: Heart and Vessels

    doi: 10.1007/s00380-016-0891-1

    Western blot analyzed the expression of Bax, Bcl-2. All data are shown as mean ± SEM. *** p
    Figure Legend Snippet: Western blot analyzed the expression of Bax, Bcl-2. All data are shown as mean ± SEM. *** p

    Techniques Used: Western Blot, Expressing

    33) Product Images from "Hepatitis E Virus Induces Brain Injury Probably Associated With Mitochondrial Apoptosis"

    Article Title: Hepatitis E Virus Induces Brain Injury Probably Associated With Mitochondrial Apoptosis

    Journal: Frontiers in Cellular and Infection Microbiology

    doi: 10.3389/fcimb.2019.00433

    Pro-apoptotic protein Bax but not Bcl-2 was upregulated following HEV infection. (A–D) HEV-RNA positive brain tissues collected on 14, 21, and 28 dpi were used for the immunohistochemistry study of Bax (rabbit polyclonal IgG) and Bcl-2(rabbit polyclonal IgG). Goat anti-rabbit IgG was chosen as secondary antibody. The positive signal was measured via the Motic Med 6.0 CMIAS Image Analysis System. Data showed that Bax was mainly distributed in cytosol of neuron cells, vascular endothelial cells and few microglial cells of HEV infection tissues with increased amount compared with mock group (* p
    Figure Legend Snippet: Pro-apoptotic protein Bax but not Bcl-2 was upregulated following HEV infection. (A–D) HEV-RNA positive brain tissues collected on 14, 21, and 28 dpi were used for the immunohistochemistry study of Bax (rabbit polyclonal IgG) and Bcl-2(rabbit polyclonal IgG). Goat anti-rabbit IgG was chosen as secondary antibody. The positive signal was measured via the Motic Med 6.0 CMIAS Image Analysis System. Data showed that Bax was mainly distributed in cytosol of neuron cells, vascular endothelial cells and few microglial cells of HEV infection tissues with increased amount compared with mock group (* p

    Techniques Used: Infection, Immunohistochemistry

    34) Product Images from "The Toxicity Mechanisms of Action of Aβ25–35 in Isolated Rat Cardiac Myocytes"

    Article Title: The Toxicity Mechanisms of Action of Aβ25–35 in Isolated Rat Cardiac Myocytes

    Journal: Molecules

    doi: 10.3390/molecules190812242

    ( A ) Aβ 25–35 induced the expression of the apoptosis protein in the cardiac myocyte; ( B ) Aβ 25–35 induced the expression of ER stress marker in the cardiac myocyte. Cells were treated with indicated concentrations of Aβ 25–35 for 24 h and subjected to western blotting to measure the related protein; ( C – D ) Relative protein levels were quantified by densitometry and shown in the histogram; ( E ) The ratio of the Bax/Bcl-2; ( F ) Aβ 25–35 decreased the expression ROCK protein. Values are expressed as mean ± SEM of three independent experiments, each in triplicate. * p
    Figure Legend Snippet: ( A ) Aβ 25–35 induced the expression of the apoptosis protein in the cardiac myocyte; ( B ) Aβ 25–35 induced the expression of ER stress marker in the cardiac myocyte. Cells were treated with indicated concentrations of Aβ 25–35 for 24 h and subjected to western blotting to measure the related protein; ( C – D ) Relative protein levels were quantified by densitometry and shown in the histogram; ( E ) The ratio of the Bax/Bcl-2; ( F ) Aβ 25–35 decreased the expression ROCK protein. Values are expressed as mean ± SEM of three independent experiments, each in triplicate. * p

    Techniques Used: Expressing, Marker, Western Blot

    35) Product Images from "Down-regulation of long non-coding RNA ESCCAL_1 inhibits tumor growth of esophageal squamous cell carcinoma in a xenograft mouse model"

    Article Title: Down-regulation of long non-coding RNA ESCCAL_1 inhibits tumor growth of esophageal squamous cell carcinoma in a xenograft mouse model

    Journal: Oncotarget

    doi: 10.18632/oncotarget.23153

    Diagram illustrates the underlying mechanism of ESCCAL_1 knockdown inhibits tumor growth in nude mice From the phenotype of overexpression of ESCCAL_1 promoting tumor growth to targets screening of phospho-kinase protein array, along with shRNA knockdown of ESCCAL_1 expression, the cross-talk of multiple signaling pathways may participate in ESCCAL_1-mediated oncogenesis. The inactivation of p-Src, p-FAK, p-JNK1, decrease of Bcl-2 expression and increase of p-p38, p53, BAX and Caspase-3 expression in ESCCAL_1 KD group were detected in this study (Red arrows: decrease; white arrows: increase; black arrows: activate/induce).
    Figure Legend Snippet: Diagram illustrates the underlying mechanism of ESCCAL_1 knockdown inhibits tumor growth in nude mice From the phenotype of overexpression of ESCCAL_1 promoting tumor growth to targets screening of phospho-kinase protein array, along with shRNA knockdown of ESCCAL_1 expression, the cross-talk of multiple signaling pathways may participate in ESCCAL_1-mediated oncogenesis. The inactivation of p-Src, p-FAK, p-JNK1, decrease of Bcl-2 expression and increase of p-p38, p53, BAX and Caspase-3 expression in ESCCAL_1 KD group were detected in this study (Red arrows: decrease; white arrows: increase; black arrows: activate/induce).

    Techniques Used: Mouse Assay, Over Expression, Protein Array, shRNA, Expressing

    Over-expression of ESCCAL_1 promotes survival and suppresses apoptosis ( A , C ) Protein levels of p-p38α, total p38α (t-p38α), p53, Bcl-2, BAX and Caspase-3 were detected by Western blot. ( B , D ) Quantitative protein levels of p-p38α, p53, Bcl-2, BAX and Caspase-3 were normalized with t-p38α or β-actin. Data are presented as mean ± SEM. n = 5 per group. * p
    Figure Legend Snippet: Over-expression of ESCCAL_1 promotes survival and suppresses apoptosis ( A , C ) Protein levels of p-p38α, total p38α (t-p38α), p53, Bcl-2, BAX and Caspase-3 were detected by Western blot. ( B , D ) Quantitative protein levels of p-p38α, p53, Bcl-2, BAX and Caspase-3 were normalized with t-p38α or β-actin. Data are presented as mean ± SEM. n = 5 per group. * p

    Techniques Used: Over Expression, Western Blot

    36) Product Images from "H2S attenuates sepsis-induced cardiac dysfunction via a PI3K/Akt-dependent mechanism"

    Article Title: H2S attenuates sepsis-induced cardiac dysfunction via a PI3K/Akt-dependent mechanism

    Journal: Experimental and Therapeutic Medicine

    doi: 10.3892/etm.2019.7440

    Expression of NF-κB, Bcl-2, Bax and caspase in each group. Representative western blotting images are presented. Lanes: a, Sham; b, sham + NaHS; c, sham + LY; d, CLP; e, CLP + NaHS; f, CLP + LY; g, CLP + NaHS + LY. Quantified data are expressed as the mean ± standard error. *P
    Figure Legend Snippet: Expression of NF-κB, Bcl-2, Bax and caspase in each group. Representative western blotting images are presented. Lanes: a, Sham; b, sham + NaHS; c, sham + LY; d, CLP; e, CLP + NaHS; f, CLP + LY; g, CLP + NaHS + LY. Quantified data are expressed as the mean ± standard error. *P

    Techniques Used: Expressing, Western Blot

    37) Product Images from "Notch-1 Signaling Promotes the Malignant Features of Human Breast Cancer through NF-?B Activation"

    Article Title: Notch-1 Signaling Promotes the Malignant Features of Human Breast Cancer through NF-?B Activation

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0095912

    Effect of Notch-1 signaling on the expression of Hes-1, cyclin D1, Bcl-xL, and survivin. The expression of Hes-1, cyclin D1, Bcl-xL, and survivin protein was detected by western blotting analysis. The β-actin was used as a loading control.
    Figure Legend Snippet: Effect of Notch-1 signaling on the expression of Hes-1, cyclin D1, Bcl-xL, and survivin. The expression of Hes-1, cyclin D1, Bcl-xL, and survivin protein was detected by western blotting analysis. The β-actin was used as a loading control.

    Techniques Used: Expressing, Western Blot

    38) Product Images from "Alpha-lipoic acid attenuates trinitrobenzene sulfonic acid-induced ulcerative colitis in mice"

    Article Title: Alpha-lipoic acid attenuates trinitrobenzene sulfonic acid-induced ulcerative colitis in mice

    Journal: International Journal of Clinical and Experimental Medicine

    doi:

    Effects of ALA on TNBS-induced apoptosis in colon. TUNEL staining of colon from mice sacrificed 7 days after TNBS-induced UC (A). Bax and Bcl-2 representative blots are shown and the protein size is expressed in kDa (B). Protein levels were calculated
    Figure Legend Snippet: Effects of ALA on TNBS-induced apoptosis in colon. TUNEL staining of colon from mice sacrificed 7 days after TNBS-induced UC (A). Bax and Bcl-2 representative blots are shown and the protein size is expressed in kDa (B). Protein levels were calculated

    Techniques Used: TUNEL Assay, Staining, Mouse Assay

    39) Product Images from "Pathway of Programmed Cell Death and Oxidative Stress Induced by ?-Hydroxybutyrate in Dairy Cow Abomasum Smooth Muscle Cells and in Mouse Gastric Smooth Muscle"

    Article Title: Pathway of Programmed Cell Death and Oxidative Stress Induced by ?-Hydroxybutyrate in Dairy Cow Abomasum Smooth Muscle Cells and in Mouse Gastric Smooth Muscle

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0096775

    Effects of β-HB on the expression of apoptosis proteins in vivo . Mice were intraperitoneally given β-HB or PBS (control). Cleaved caspase-12, - 9, - 3, Bax, Bcl-2, AIF and EndoG protein were detected by Western blot.
    Figure Legend Snippet: Effects of β-HB on the expression of apoptosis proteins in vivo . Mice were intraperitoneally given β-HB or PBS (control). Cleaved caspase-12, - 9, - 3, Bax, Bcl-2, AIF and EndoG protein were detected by Western blot.

    Techniques Used: Expressing, In Vivo, Mouse Assay, Western Blot

    Effect of β-HB on the expression of cleaved caspase-12, -9, -3 Bax, Bcl-2 in BSMCs. A) and B) Cells were incubated 48 h with β-HB (0, 0.6, 1.2, 2.4 or 4.8 mM) in serum-free medium. Cleaved caspase-12, - 9, - 3, Bax and Bcl-2 were detected by Western blot. C) Cells were incubated 48 h with β-HB (0, 2.4 mM) in serum-free medium in the absence or presence of NAC (1 mM) or BAPTA/AM (15 µM). Cleaved caspase-12, - 9, - 3 were detected by Western blot.
    Figure Legend Snippet: Effect of β-HB on the expression of cleaved caspase-12, -9, -3 Bax, Bcl-2 in BSMCs. A) and B) Cells were incubated 48 h with β-HB (0, 0.6, 1.2, 2.4 or 4.8 mM) in serum-free medium. Cleaved caspase-12, - 9, - 3, Bax and Bcl-2 were detected by Western blot. C) Cells were incubated 48 h with β-HB (0, 2.4 mM) in serum-free medium in the absence or presence of NAC (1 mM) or BAPTA/AM (15 µM). Cleaved caspase-12, - 9, - 3 were detected by Western blot.

    Techniques Used: Expressing, Incubation, Western Blot

    40) Product Images from "Nek7 is overexpressed in hepatocellular carcinoma and promotes hepatocellular carcinoma cell proliferation in vitro and in vivo"

    Article Title: Nek7 is overexpressed in hepatocellular carcinoma and promotes hepatocellular carcinoma cell proliferation in vitro and in vivo

    Journal: Oncotarget

    doi: 10.18632/oncotarget.7620

    Nek7 gene silencing suppressed growth of SMMC7721 xenograft tumors A. Tumors isolated from mice of different groups are shown. B. Growth curves of xenograft tumors from the experiments with BALB/c nude mice. Changes in tumor volumes measured at the indicated days are shown. C. Therapeutic lenti-shNek7-1 reduced the tumor weight. Real-time PCR and Western blot assays to determine the mRNA and protein levels of cell growth associated genes, Bcl-2, cyclinD1 and cyclinB1 in HCC cells D. and dissected tumors E, F. Immunohistochemical staining was used to detect the Nek7 and Ki-67 expression pattern in xenograft tumors from different groups. β-actin was used as internal control for Western blot and Real-time PCR. Experiments were repeated three times. (* p
    Figure Legend Snippet: Nek7 gene silencing suppressed growth of SMMC7721 xenograft tumors A. Tumors isolated from mice of different groups are shown. B. Growth curves of xenograft tumors from the experiments with BALB/c nude mice. Changes in tumor volumes measured at the indicated days are shown. C. Therapeutic lenti-shNek7-1 reduced the tumor weight. Real-time PCR and Western blot assays to determine the mRNA and protein levels of cell growth associated genes, Bcl-2, cyclinD1 and cyclinB1 in HCC cells D. and dissected tumors E, F. Immunohistochemical staining was used to detect the Nek7 and Ki-67 expression pattern in xenograft tumors from different groups. β-actin was used as internal control for Western blot and Real-time PCR. Experiments were repeated three times. (* p

    Techniques Used: Isolation, Mouse Assay, Real-time Polymerase Chain Reaction, Western Blot, Immunohistochemistry, Staining, Expressing

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    In Situ:

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    Apoptosis of HeLa-vect and HeLa-TRAIL cells treated with Taxol. (A) AO/EB-stained cells. Scale bar, 100 µm. (B) Western blot analysis of cleaved caspase-3 and <t>Bcl-2</t> protein expression. (C) Quantification of cleaved caspase-3 and Bcl-2 protein levels from three experiments. (D) DR5 mRNA levels in HeLa cells treated with different concentrations of Taxol. Data are from three separate experiments. Data are presented as the means ± SD. **P
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    Apoptosis of HeLa-vect and HeLa-TRAIL cells treated with Taxol. (A) AO/EB-stained cells. Scale bar, 100 µm. (B) Western blot analysis of cleaved caspase-3 and Bcl-2 protein expression. (C) Quantification of cleaved caspase-3 and Bcl-2 protein levels from three experiments. (D) DR5 mRNA levels in HeLa cells treated with different concentrations of Taxol. Data are from three separate experiments. Data are presented as the means ± SD. **P

    Journal: Oncology Letters

    Article Title: Tumor necrosis factor-related apoptosis inducing ligand overexpression and Taxol treatment suppresses the growth of cervical cancer cells in vitro and in vivo

    doi: 10.3892/ol.2018.8071

    Figure Lengend Snippet: Apoptosis of HeLa-vect and HeLa-TRAIL cells treated with Taxol. (A) AO/EB-stained cells. Scale bar, 100 µm. (B) Western blot analysis of cleaved caspase-3 and Bcl-2 protein expression. (C) Quantification of cleaved caspase-3 and Bcl-2 protein levels from three experiments. (D) DR5 mRNA levels in HeLa cells treated with different concentrations of Taxol. Data are from three separate experiments. Data are presented as the means ± SD. **P

    Article Snippet: Antibodies against β-actin, Bcl-2, cleaved caspase-3, and TRAIL were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).

    Techniques: Staining, Western Blot, Expressing

    H E, TUNEL, and immunohistochemical staining of HeLa tumor xenografts. (A, B) H E and TUNEL staining. (C, D) Immunohistochemical staining of cleaved caspase-3 (C) and Bcl-2 (D). Scale bar, 100 µm). (E) Quantification of the percentage of apoptotic cells in the TUNEL assay. (F) Quantification of cleaved caspase-3 and Bcl-2 protein expression detected by immunohistochemistry. Data are presented as the means ± SD. **P

    Journal: Oncology Letters

    Article Title: Tumor necrosis factor-related apoptosis inducing ligand overexpression and Taxol treatment suppresses the growth of cervical cancer cells in vitro and in vivo

    doi: 10.3892/ol.2018.8071

    Figure Lengend Snippet: H E, TUNEL, and immunohistochemical staining of HeLa tumor xenografts. (A, B) H E and TUNEL staining. (C, D) Immunohistochemical staining of cleaved caspase-3 (C) and Bcl-2 (D). Scale bar, 100 µm). (E) Quantification of the percentage of apoptotic cells in the TUNEL assay. (F) Quantification of cleaved caspase-3 and Bcl-2 protein expression detected by immunohistochemistry. Data are presented as the means ± SD. **P

    Article Snippet: Antibodies against β-actin, Bcl-2, cleaved caspase-3, and TRAIL were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).

    Techniques: TUNEL Assay, Immunohistochemistry, Staining, Expressing

    Underlying mechanisms of apoptosis induced by positron in SW1990 pancreatic cancer cells Four groups cells were incubated with different concentrations 18 F-FDG (a) control group; (b) 18.5MBq/ml; (c) 37MBq/ml; (d) 74MBq/ml. (A) Real-time RT-PCR analysis for the mRNA expression of apotosis-related genes. Positron decreased Bcl-2 mRNA expression and increased Bax, Caspase-3, Caspase-9 and P53 mRNA expression in SW1990 cells in a dose-dependent manner. (B) Western blotting analysis for the Cleaved Caspase-3, Cytochrome C and Cleaved Caspase-9 protein treated with different concentrations 18 F-FDG for 24h. (C) statistical histogram of western blotting. The data were expressed as mean±SEM obtained from three independent samples. * p

    Journal: Oncotarget

    Article Title: Experimental study on the therapeutic effect and underlining mechanisms of positron in pancreatic cancer cells

    doi: 10.18632/oncotarget.18366

    Figure Lengend Snippet: Underlying mechanisms of apoptosis induced by positron in SW1990 pancreatic cancer cells Four groups cells were incubated with different concentrations 18 F-FDG (a) control group; (b) 18.5MBq/ml; (c) 37MBq/ml; (d) 74MBq/ml. (A) Real-time RT-PCR analysis for the mRNA expression of apotosis-related genes. Positron decreased Bcl-2 mRNA expression and increased Bax, Caspase-3, Caspase-9 and P53 mRNA expression in SW1990 cells in a dose-dependent manner. (B) Western blotting analysis for the Cleaved Caspase-3, Cytochrome C and Cleaved Caspase-9 protein treated with different concentrations 18 F-FDG for 24h. (C) statistical histogram of western blotting. The data were expressed as mean±SEM obtained from three independent samples. * p

    Article Snippet: The rabbit polyclonal antibodies specific for Bcl-2, Bax, Survivin, Caspase-3, Caspase-9 and Cytochome C were purchased from Boster (Wuhan, China).

    Techniques: Incubation, Quantitative RT-PCR, Expressing, Western Blot

    Expression of Bcl-2 and Bax in GMT-treated Sprague-Dawley rats with acute myocardial infarction. (A and B) Western Blot was used to evaluate the protein expression of Bcl-2 and Bax. (C) Immunohistochemical analysis was used to detected the expression of Bcl-2 and Bax (magnification, ×400). # P

    Journal: Experimental and Therapeutic Medicine

    Article Title: Cardioprotective effects of traditional Chinese medicine Guanmaitong on acute myocardial infarction

    doi: 10.3892/etm.2016.3888

    Figure Lengend Snippet: Expression of Bcl-2 and Bax in GMT-treated Sprague-Dawley rats with acute myocardial infarction. (A and B) Western Blot was used to evaluate the protein expression of Bcl-2 and Bax. (C) Immunohistochemical analysis was used to detected the expression of Bcl-2 and Bax (magnification, ×400). # P

    Article Snippet: Sections were incubated overnight at 4°C with primary anti-Bcl-2 (BA0412) and anti-Bax (BA0315) antibodies (both 1:500; Wuhan Boster Biological Technology Ltd., Wuhan, China).

    Techniques: Expressing, Western Blot, Immunohistochemistry

    Ibutilide treatment attenuates tunicamycin induced increase in Bax/ Bcl-2 ratio. Protein expression of Bax and Bcl-2 were analyzed from lysate derived from RNC treated with either tunicamycin alone (model), tunicamycin and ibutilide (treatment) or untreated cardiomyocytes (control). Representative immunoblots of lysate immunoblotted with antibodies specific for Bax or BCL-2 are shown. The quantification of normalized band densitometry of Bax was divided by that of Bcl-2 and the results are graphed. All data are shown as mean ± SE ( n = 3 per group). *p

    Journal: PLoS ONE

    Article Title: Ibutilide treatment protects against ER stress induced apoptosis by regulating calumenin expression in tunicamycin treated cardiomyocytes

    doi: 10.1371/journal.pone.0173469

    Figure Lengend Snippet: Ibutilide treatment attenuates tunicamycin induced increase in Bax/ Bcl-2 ratio. Protein expression of Bax and Bcl-2 were analyzed from lysate derived from RNC treated with either tunicamycin alone (model), tunicamycin and ibutilide (treatment) or untreated cardiomyocytes (control). Representative immunoblots of lysate immunoblotted with antibodies specific for Bax or BCL-2 are shown. The quantification of normalized band densitometry of Bax was divided by that of Bcl-2 and the results are graphed. All data are shown as mean ± SE ( n = 3 per group). *p

    Article Snippet: Antibodies for anti-calumenin (1: 500), anti-caspase-3 (1: 500), anti-caspase-9 (1: 500), anti-caspase-12 (1: 500) were obtained from Bioss, anti-CHOP (1: 1000), anti-Bcl-2 (1: 1000) were obtained from Wanleibio, and anti-GRP78 (1: 400), anti-GRP94 (1: 400), anti-Bax (1: 400), anti-ATF-6 (1: 400) were purchased from Boster, and anti-p-PERK (1:750), anti-PERK (1:750), anti-spliced XBP-1 (1:500), anti-unspliced XBP-1 (1:500) were obtained from Abcam.

    Techniques: Expressing, Derivative Assay, Western Blot