thermolabile user ii enzyme  (New England Biolabs)


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    New England Biolabs thermolabile user ii enzyme
    Thermolabile User Ii Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    thermolabile user ii enzyme  (New England Biolabs)


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    New England Biolabs thermolabile user ii enzyme
    Thermolabile User Ii Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    thermolabile user ii enzyme  (New England Biolabs)


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    New England Biolabs thermolabile user ii enzyme
    Thermolabile User Ii Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    user enzyme  (New England Biolabs)


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    New England Biolabs user enzyme
    User Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    m5508  (New England Biolabs)


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    New England Biolabs m5508
    KEY RESOURCES TABLE
    M5508, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    1) Product Images from "Stromal remodeling regulates dendritic cell abundance and activity in the tumor microenvironment"

    Article Title: Stromal remodeling regulates dendritic cell abundance and activity in the tumor microenvironment

    Journal: Cell reports

    doi: 10.1016/j.celrep.2022.111201

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Flow Cytometry, shRNA, Recombinant, Plasmid Preparation, Western Blot, Electron Microscopy, Activation Assay, DNA Purification, SYBR Green Assay, Endotoxin Assay, Software, Cell Counting, Staining

    thermolabile user enzyme  (New England Biolabs)


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    New England Biolabs thermolabile user enzyme
    Thermolabile User Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermolabile user enzyme/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
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    thermolabile user ii enzyme  (New England Biolabs)


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    New England Biolabs thermolabile user ii enzyme
    Thermolabile User Ii Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermolabile user ii enzyme/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
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    thermolabile user ii enzyme  (New England Biolabs)


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    New England Biolabs thermolabile user ii enzyme
    Thermolabile User Ii Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermolabile user ii enzyme/product/New England Biolabs
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    thermolabile user ii enzyme  (New England Biolabs)


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    New England Biolabs thermolabile user ii enzyme
    Thermolabile User Ii Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    thermolabile user ii enzyme  (New England Biolabs)


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    New England Biolabs thermolabile user ii enzyme
    <t>USER</t> footprinting to assess promoter accessibility. A , structure of Thermus aquaticus initiation complex (PDB: 4XLN) annotated to indicate the position of P RA1 dU bases relative to direct contacts between σ and <t>promoter</t> <t>DNA.</t> B , USER footprinting assay to assess P RA1 promoter accessibility. Saturation of the P RA1 promoter with open complexes (conditions from <xref ref-type=Fig. 2 A ) attenuates USER digestion. TECs prepared under conditions that predominantly yield slow-migrating complexes ( Fig. 4 B , lane 2) inhibit USER digestion, indicating promoter occupation by open complexes. TECs prepared under conditions that yield >97% fast-migrating complexes ( Fig. 4 B , lane 6) are efficiently digested, indicating the absence of open complexes. The grayscale on the lower cut-out is adjusted to better show the RNA band; the full gel is shown with this darker grayscale setting in Fig. S6 D . C , quantification of USER digestion for key lanes from panel B as the ratio of USER digested to full-length band intensity. The gel shown in panel B is representative of two independent replicates. TEC, transcription elongation complex. " width="250" height="auto" />
    Thermolabile User Ii Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermolabile user ii enzyme/product/New England Biolabs
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    1) Product Images from "Preparation of E. coli RNA polymerase transcription elongation complexes by selective photoelution from magnetic beads"

    Article Title: Preparation of E. coli RNA polymerase transcription elongation complexes by selective photoelution from magnetic beads

    Journal: The Journal of Biological Chemistry

    doi: 10.1016/j.jbc.2021.100812

    USER footprinting to assess promoter accessibility. A , structure of Thermus aquaticus initiation complex (PDB: 4XLN) annotated to indicate the position of P RA1 dU bases relative to direct contacts between σ and promoter DNA. B , USER footprinting assay to assess P RA1 promoter accessibility. Saturation of the P RA1 promoter with open complexes (conditions from <xref ref-type=Fig. 2 A ) attenuates USER digestion. TECs prepared under conditions that predominantly yield slow-migrating complexes ( Fig. 4 B , lane 2) inhibit USER digestion, indicating promoter occupation by open complexes. TECs prepared under conditions that yield >97% fast-migrating complexes ( Fig. 4 B , lane 6) are efficiently digested, indicating the absence of open complexes. The grayscale on the lower cut-out is adjusted to better show the RNA band; the full gel is shown with this darker grayscale setting in Fig. S6 D . C , quantification of USER digestion for key lanes from panel B as the ratio of USER digested to full-length band intensity. The gel shown in panel B is representative of two independent replicates. TEC, transcription elongation complex. " title="USER footprinting to assess promoter accessibility. A , structure ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: USER footprinting to assess promoter accessibility. A , structure of Thermus aquaticus initiation complex (PDB: 4XLN) annotated to indicate the position of P RA1 dU bases relative to direct contacts between σ and promoter DNA. B , USER footprinting assay to assess P RA1 promoter accessibility. Saturation of the P RA1 promoter with open complexes (conditions from Fig. 2 A ) attenuates USER digestion. TECs prepared under conditions that predominantly yield slow-migrating complexes ( Fig. 4 B , lane 2) inhibit USER digestion, indicating promoter occupation by open complexes. TECs prepared under conditions that yield >97% fast-migrating complexes ( Fig. 4 B , lane 6) are efficiently digested, indicating the absence of open complexes. The grayscale on the lower cut-out is adjusted to better show the RNA band; the full gel is shown with this darker grayscale setting in Fig. S6 D . C , quantification of USER digestion for key lanes from panel B as the ratio of USER digested to full-length band intensity. The gel shown in panel B is representative of two independent replicates. TEC, transcription elongation complex.

    Techniques Used: Footprinting

    user enzyme  (New England Biolabs)


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    New England Biolabs user enzyme
    User Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs thermolabile user ii enzyme
    Thermolabile User Ii Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs m5508
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    New England Biolabs thermolabile user enzyme
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    Thermolabile User Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Stromal remodeling regulates dendritic cell abundance and activity in the tumor microenvironment

    doi: 10.1016/j.celrep.2022.111201

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: USER® Enzyme , New England Biolabs , M5508.

    Techniques: Flow Cytometry, shRNA, Recombinant, Plasmid Preparation, Western Blot, Electron Microscopy, Activation Assay, DNA Purification, SYBR Green Assay, Endotoxin Assay, Software, Cell Counting, Staining