luna universal probe qpcr master mix  (New England Biolabs)


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    Name:
    Luna Universal Probe qPCR Master Mix
    Description:
    Luna Universal Probe qPCR Master Mix 2 500 rxns
    Catalog Number:
    m3004e
    Price:
    877
    Size:
    2 500 rxns
    Category:
    PCR Product Visualization Kits
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    New England Biolabs luna universal probe qpcr master mix
    Luna Universal Probe qPCR Master Mix
    Luna Universal Probe qPCR Master Mix 2 500 rxns
    https://www.bioz.com/result/luna universal probe qpcr master mix/product/New England Biolabs
    Average 95 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    luna universal probe qpcr master mix - by Bioz Stars, 2020-05
    95/100 stars

    Images

    1) Product Images from "A Small Interfering RNA Cocktail Targeting the Nucleoprotein and Large Protein Genes Suppresses Borna Disease Virus Infection"

    Article Title: A Small Interfering RNA Cocktail Targeting the Nucleoprotein and Large Protein Genes Suppresses Borna Disease Virus Infection

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2019.02781

    Reduction in the BoDV-1 load by siRNAs targeting N and M/G/L mRNAs. (A,B) Effects of siRNAs targeting BoDV-1 mRNAs on BoDV-1 mRNA amounts. 293T/BoDV cells were treated with the indicated siRNA for 2 days. The amounts of N (A) and M/G/L (B) mRNAs in 293T/BoDV cells were determined by RT-qPCR analyses. (C) Effects of siRNAs targeting BoDV-1 mRNAs on BoDV-1 replication. The amount of BoDV-1 gRNA in 293T/BoDV cells was determined by RT-qPCR analyses. (D,E) Effects of the siRNA cocktail (TD-Borna) targeting BoDV-1 N and M/G/L mRNAs on BoDV-1 mRNAs. 293T/BoDV cells were treated with TD-Borna for 2 days. The amounts of N (D) and M/G/L (E) mRNAs in 293T/BoDV cells were determined by RT-qPCR analyses. (F) Effects of TD-Borna on BoDV-1 replication. The amount of BoDV-1 gRNA in 293T/BoDV cells was determined by RT-qPCR analyses. (G) Protocol for long-term TD-Borna treatment in 293T/BoDV cells. 293T/BoDV cells were seeded in 12-well plates (2 × 10 5 cells/well) on Day –1. TD-Borna was transfected on Days 0, 6, and 12. The cells were passaged into new 12-well plates (2 × 10 5 cells/well) every 3 days. (H) Effects of long-term TD-Borna treatment on BoDV-1 replication. The amount of BoDV-1 gRNA in 293T/BoDV cells was determined by RT-qPCR analyses. Mock, the scrambled siRNA-treated control. Values are expressed as the mean ± SE of three independent experiments. ∗∗ P
    Figure Legend Snippet: Reduction in the BoDV-1 load by siRNAs targeting N and M/G/L mRNAs. (A,B) Effects of siRNAs targeting BoDV-1 mRNAs on BoDV-1 mRNA amounts. 293T/BoDV cells were treated with the indicated siRNA for 2 days. The amounts of N (A) and M/G/L (B) mRNAs in 293T/BoDV cells were determined by RT-qPCR analyses. (C) Effects of siRNAs targeting BoDV-1 mRNAs on BoDV-1 replication. The amount of BoDV-1 gRNA in 293T/BoDV cells was determined by RT-qPCR analyses. (D,E) Effects of the siRNA cocktail (TD-Borna) targeting BoDV-1 N and M/G/L mRNAs on BoDV-1 mRNAs. 293T/BoDV cells were treated with TD-Borna for 2 days. The amounts of N (D) and M/G/L (E) mRNAs in 293T/BoDV cells were determined by RT-qPCR analyses. (F) Effects of TD-Borna on BoDV-1 replication. The amount of BoDV-1 gRNA in 293T/BoDV cells was determined by RT-qPCR analyses. (G) Protocol for long-term TD-Borna treatment in 293T/BoDV cells. 293T/BoDV cells were seeded in 12-well plates (2 × 10 5 cells/well) on Day –1. TD-Borna was transfected on Days 0, 6, and 12. The cells were passaged into new 12-well plates (2 × 10 5 cells/well) every 3 days. (H) Effects of long-term TD-Borna treatment on BoDV-1 replication. The amount of BoDV-1 gRNA in 293T/BoDV cells was determined by RT-qPCR analyses. Mock, the scrambled siRNA-treated control. Values are expressed as the mean ± SE of three independent experiments. ∗∗ P

    Techniques Used: Quantitative RT-PCR, Transfection

    Reduction in the BoDV-1 load by the combined use of T-705 and TD-Borna. (A) Protocol for long-term treatment using T-705 and TD-Borna in 293T/BoDV cells. 293T/BoDV cells were seeded in 12-well plates (2 × 10 5 cells/well) on Day –1. TD-Borna was transfected on Days 0 and 6. On Day 0, T-705 was added at 1 h after the transfection. The cells were passaged into new 12-well plates (2 × 10 5 cells/well) with or without T-705 on Days 2, 5, and 8. (B) Effects of long-term treatment with T-705 and TD-Borna on BoDV-1 replication. The amount of BoDV-1 gRNA in 293T/BoDV cells was determined by RT-qPCR analyses. (C) The amount of the N protein in 293T/BoDV cells treated with T-705 and TD-Borna. 293T/BoDV cells were treated as indicated in panel (A) . After 5 days of treatment, the amount of the N protein was determined by western blotting using anti-N and anti-tubulin antibodies. (D) Quantification of the amount of the N protein in panel (C) . The band intensity of the N protein in each sample was normalized with that of tubulin. Mock, the scrambled siRNA-treated control. Values are expressed as the mean ± SE of three independent experiments. ∗ P
    Figure Legend Snippet: Reduction in the BoDV-1 load by the combined use of T-705 and TD-Borna. (A) Protocol for long-term treatment using T-705 and TD-Borna in 293T/BoDV cells. 293T/BoDV cells were seeded in 12-well plates (2 × 10 5 cells/well) on Day –1. TD-Borna was transfected on Days 0 and 6. On Day 0, T-705 was added at 1 h after the transfection. The cells were passaged into new 12-well plates (2 × 10 5 cells/well) with or without T-705 on Days 2, 5, and 8. (B) Effects of long-term treatment with T-705 and TD-Borna on BoDV-1 replication. The amount of BoDV-1 gRNA in 293T/BoDV cells was determined by RT-qPCR analyses. (C) The amount of the N protein in 293T/BoDV cells treated with T-705 and TD-Borna. 293T/BoDV cells were treated as indicated in panel (A) . After 5 days of treatment, the amount of the N protein was determined by western blotting using anti-N and anti-tubulin antibodies. (D) Quantification of the amount of the N protein in panel (C) . The band intensity of the N protein in each sample was normalized with that of tubulin. Mock, the scrambled siRNA-treated control. Values are expressed as the mean ± SE of three independent experiments. ∗ P

    Techniques Used: Transfection, Quantitative RT-PCR, Western Blot

    Reduction in the BoDV-1 load by TD-Borna in neuronal and non-neuronal cell lines. (A,B) Effect of T-705 on BoDV-1 replication in 293T/BoDV (A) and SH-SY5Y/BoDV (B) cells. (C,D) Effect of TD-Borna on BoDV-1 replication in 293T/BoDV (C) and SH-SY5Y/BoDV (D) cells. The cells were treated with T-705 (A,B) or TD-Borna (C,D) for 2 days. The amount of BoDV-1 gRNA in the cells was determined by RT-qPCR analyses. Mock, the scrambled siRNA-treated control. Values are expressed as the mean ± SE of three independent experiments. **** P
    Figure Legend Snippet: Reduction in the BoDV-1 load by TD-Borna in neuronal and non-neuronal cell lines. (A,B) Effect of T-705 on BoDV-1 replication in 293T/BoDV (A) and SH-SY5Y/BoDV (B) cells. (C,D) Effect of TD-Borna on BoDV-1 replication in 293T/BoDV (C) and SH-SY5Y/BoDV (D) cells. The cells were treated with T-705 (A,B) or TD-Borna (C,D) for 2 days. The amount of BoDV-1 gRNA in the cells was determined by RT-qPCR analyses. Mock, the scrambled siRNA-treated control. Values are expressed as the mean ± SE of three independent experiments. **** P

    Techniques Used: Quantitative RT-PCR

    2) Product Images from "Molecular monitoring of the diversity of human pathogenic malaria species in blood donations on Bioko Island, Equatorial Guinea"

    Article Title: Molecular monitoring of the diversity of human pathogenic malaria species in blood donations on Bioko Island, Equatorial Guinea

    Journal: Malaria Journal

    doi: 10.1186/s12936-019-2639-8

    Analytical performance of PlasQ ( a ) and PlasID ( b ) assay. a Correlation of P. falciparum standards and the Cq values for both targets, Pspp18S (black circles) and PfvarATS (white circles) of the PlasQ assay. Based on these quadruple replicates of the WHO standards, LODs and qPCR efficiencies were calculated. b The ability of the PlasID assay identifying five different malaria species is shown by a representative, composite amplification plot. DNA from the six Plasmodium species were analysed in separate tubes during the same qPCR experiment
    Figure Legend Snippet: Analytical performance of PlasQ ( a ) and PlasID ( b ) assay. a Correlation of P. falciparum standards and the Cq values for both targets, Pspp18S (black circles) and PfvarATS (white circles) of the PlasQ assay. Based on these quadruple replicates of the WHO standards, LODs and qPCR efficiencies were calculated. b The ability of the PlasID assay identifying five different malaria species is shown by a representative, composite amplification plot. DNA from the six Plasmodium species were analysed in separate tubes during the same qPCR experiment

    Techniques Used: Real-time Polymerase Chain Reaction, Amplification

    Related Articles

    Quantitative RT-PCR:

    Article Title: A Small Interfering RNA Cocktail Targeting the Nucleoprotein and Large Protein Genes Suppresses Borna Disease Virus Infection
    Article Snippet: .. RT-qPCR assays for BoDV-1 L mRNA and BoDV-1 gRNA were carried out using Luna Universal Probe qPCR Master Mix (New England Biolabs, Ipswich, United States) and the BoDV-1-specific primers and probe. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Real-time analysis of nanopore-based metagenomic sequencing from infected orthopaedic devices
    Article Snippet: .. Quantitative real-time PCR (q-PCR) was performed for sample 354a to determine relative amounts of both Arcanobacterium haemolyticum and Fusobacterium nucleatum DNA in the original sonication fluid genomic DNA extract. qPCR was performed on a Stratagene MX3005P QPCR System (Agilent Technologies, Santa Clara, CA, USA) using Luna Universal Probe qPCR Master Mix (New England Biolabs, Ipswich, MA, USA). ..

    Article Title: Adiponectin, Leptin and Visfatin in Hypoxia and its Effect for Weight Loss in Obesity
    Article Snippet: .. A TaqMan probe-based real-time PCR was used for GADPH (Luna Universal Probe qPCR Master Mix, New England Biolabs, cat. no. M3004S), subsequently, the manufacturers recommended PCR conditions were applied. .. For primer sequences of target genes, pre-designed primer-assays of Bio-Rad (Bio-Rad, Vienna, Austria) were used: qHsaCED0047448 (ADIPOQ), qHsaCID0017538 (LEP), qHsaCED0043104 (NAMPT).

    Article Title: ErbB2 Targeted Epigenetic Modulation: Anti-tumor Efficacy of the ADC Trastuzumab-HDACi ST8176AA1
    Article Snippet: .. Real time quantitative PCR analysis was performed using the Luna® Universal Probe qPCR Master Mix (New England Biolabs, Ipswich, MA, USA) and the following TaqMan Gene Expression Assays (Applied biosystems, Foster City, CA): Hs00171790_m1 [GLI-1], Hs01119974_m1 [GLI-2], Hs00181117_m1 [PTCH1], Hs00170665_m1 [SMO], Hs00960520_m1 [SUFU] and Hs00939627_m1 [for the housekeeping gene GUSB]. .. The 7900HT Sequence Detection System instrument and software (Applied Biosystems) were used to quantify the mRNA levels of the target genes, according to a six-point serial standard curve generated for each gene.

    Article Title: Resting cells rely on the DNA helicase component MCM2 to build cilia
    Article Snippet: .. Equal amounts of RNA were reversely transcribed into cDNA using oligodTTPs and Superscript II or III reverse transcriptase (Life technologies, Germany), respectively. qPCR was performed with Absolute QPCR ROX Master Mix (Thermo Fisher, Germany) or Luna Universal probe qPCR Master Mix (NEB) and the Roche Universal Probe System on a Roche LightCycler 480 using primers spanning introns whenever possible. lists all primers and probes used. .. Further information on qPCR is also given in the MIQE protocol in the supplement.

    Article Title: Molecular monitoring of the diversity of human pathogenic malaria species in blood donations on Bioko Island, Equatorial Guinea
    Article Snippet: .. Each reaction contained 2 µL DNA and 8 µL reaction master mix containing 1× Luna Universal Probe qPCR Master Mix (New England Biolabs, Ipswich, USA) and 1× PlasQ Primer Mix (Additional file ). .. All qPCR assays were run in duplicates with appropriate controls including Non-Template Control [NTC] and P. falciparum 3D7 DNA as positive control [PC].

    Article Title: A Small Interfering RNA Cocktail Targeting the Nucleoprotein and Large Protein Genes Suppresses Borna Disease Virus Infection
    Article Snippet: .. RT-qPCR assays for BoDV-1 L mRNA and BoDV-1 gRNA were carried out using Luna Universal Probe qPCR Master Mix (New England Biolabs, Ipswich, United States) and the BoDV-1-specific primers and probe. ..

    Article Title: Serum starvation of ARPE-19 changes the cellular distribution of cholesterol and Fibulin3 in patterns reminiscent of Age-related Macular Degeneration
    Article Snippet: .. Each cDNA sample was diluted to 300μl with H2 O. Quantitative PCR of selected genes was performed using the Roche Universal ProbeLibrary hydrolysis probe system (Roche, Mannheim Germany), the Luna Universal Probe qPCR Master Mix (New England BioLabs Inc. Ipswich, MA), and custom primers ( ) (Eurofins MWG|Operon) on an Applied Biosystems ViiA7 System using QuantStudio™ (v1.2) software (Life Technologies, Carlsbad, CA) following manufacturer’s recommendations. .. Relative expression values were calculated using the RQ (relative quantity) method normalized to the genes ABCF3 and NOL8.

    Expressing:

    Article Title: ErbB2 Targeted Epigenetic Modulation: Anti-tumor Efficacy of the ADC Trastuzumab-HDACi ST8176AA1
    Article Snippet: .. Real time quantitative PCR analysis was performed using the Luna® Universal Probe qPCR Master Mix (New England Biolabs, Ipswich, MA, USA) and the following TaqMan Gene Expression Assays (Applied biosystems, Foster City, CA): Hs00171790_m1 [GLI-1], Hs01119974_m1 [GLI-2], Hs00181117_m1 [PTCH1], Hs00170665_m1 [SMO], Hs00960520_m1 [SUFU] and Hs00939627_m1 [for the housekeeping gene GUSB]. .. The 7900HT Sequence Detection System instrument and software (Applied Biosystems) were used to quantify the mRNA levels of the target genes, according to a six-point serial standard curve generated for each gene.

    Polymerase Chain Reaction:

    Article Title: Adiponectin, Leptin and Visfatin in Hypoxia and its Effect for Weight Loss in Obesity
    Article Snippet: .. A TaqMan probe-based real-time PCR was used for GADPH (Luna Universal Probe qPCR Master Mix, New England Biolabs, cat. no. M3004S), subsequently, the manufacturers recommended PCR conditions were applied. .. For primer sequences of target genes, pre-designed primer-assays of Bio-Rad (Bio-Rad, Vienna, Austria) were used: qHsaCED0047448 (ADIPOQ), qHsaCID0017538 (LEP), qHsaCED0043104 (NAMPT).

    Sonication:

    Article Title: Real-time analysis of nanopore-based metagenomic sequencing from infected orthopaedic devices
    Article Snippet: .. Quantitative real-time PCR (q-PCR) was performed for sample 354a to determine relative amounts of both Arcanobacterium haemolyticum and Fusobacterium nucleatum DNA in the original sonication fluid genomic DNA extract. qPCR was performed on a Stratagene MX3005P QPCR System (Agilent Technologies, Santa Clara, CA, USA) using Luna Universal Probe qPCR Master Mix (New England Biolabs, Ipswich, MA, USA). ..

    Software:

    Article Title: Serum starvation of ARPE-19 changes the cellular distribution of cholesterol and Fibulin3 in patterns reminiscent of Age-related Macular Degeneration
    Article Snippet: .. Each cDNA sample was diluted to 300μl with H2 O. Quantitative PCR of selected genes was performed using the Roche Universal ProbeLibrary hydrolysis probe system (Roche, Mannheim Germany), the Luna Universal Probe qPCR Master Mix (New England BioLabs Inc. Ipswich, MA), and custom primers ( ) (Eurofins MWG|Operon) on an Applied Biosystems ViiA7 System using QuantStudio™ (v1.2) software (Life Technologies, Carlsbad, CA) following manufacturer’s recommendations. .. Relative expression values were calculated using the RQ (relative quantity) method normalized to the genes ABCF3 and NOL8.

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    New England Biolabs luna universal probe qpcr master mix
    Reduction in the <t>BoDV-1</t> load by siRNAs targeting N and M/G/L mRNAs. (A,B) Effects of siRNAs targeting BoDV-1 mRNAs on BoDV-1 mRNA amounts. 293T/BoDV cells were treated with the indicated siRNA for 2 days. The amounts of N (A) and M/G/L (B) mRNAs in 293T/BoDV cells were determined by <t>RT-qPCR</t> analyses. (C) Effects of siRNAs targeting BoDV-1 mRNAs on BoDV-1 replication. The amount of BoDV-1 gRNA in 293T/BoDV cells was determined by RT-qPCR analyses. (D,E) Effects of the siRNA cocktail (TD-Borna) targeting BoDV-1 N and M/G/L mRNAs on BoDV-1 mRNAs. 293T/BoDV cells were treated with TD-Borna for 2 days. The amounts of N (D) and M/G/L (E) mRNAs in 293T/BoDV cells were determined by RT-qPCR analyses. (F) Effects of TD-Borna on BoDV-1 replication. The amount of BoDV-1 gRNA in 293T/BoDV cells was determined by RT-qPCR analyses. (G) Protocol for long-term TD-Borna treatment in 293T/BoDV cells. 293T/BoDV cells were seeded in 12-well plates (2 × 10 5 cells/well) on Day –1. TD-Borna was transfected on Days 0, 6, and 12. The cells were passaged into new 12-well plates (2 × 10 5 cells/well) every 3 days. (H) Effects of long-term TD-Borna treatment on BoDV-1 replication. The amount of BoDV-1 gRNA in 293T/BoDV cells was determined by RT-qPCR analyses. Mock, the scrambled siRNA-treated control. Values are expressed as the mean ± SE of three independent experiments. ∗∗ P
    Luna Universal Probe Qpcr Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luna universal probe qpcr master mix/product/New England Biolabs
    Average 95 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    luna universal probe qpcr master mix - by Bioz Stars, 2020-05
    95/100 stars
      Buy from Supplier

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    Reduction in the BoDV-1 load by siRNAs targeting N and M/G/L mRNAs. (A,B) Effects of siRNAs targeting BoDV-1 mRNAs on BoDV-1 mRNA amounts. 293T/BoDV cells were treated with the indicated siRNA for 2 days. The amounts of N (A) and M/G/L (B) mRNAs in 293T/BoDV cells were determined by RT-qPCR analyses. (C) Effects of siRNAs targeting BoDV-1 mRNAs on BoDV-1 replication. The amount of BoDV-1 gRNA in 293T/BoDV cells was determined by RT-qPCR analyses. (D,E) Effects of the siRNA cocktail (TD-Borna) targeting BoDV-1 N and M/G/L mRNAs on BoDV-1 mRNAs. 293T/BoDV cells were treated with TD-Borna for 2 days. The amounts of N (D) and M/G/L (E) mRNAs in 293T/BoDV cells were determined by RT-qPCR analyses. (F) Effects of TD-Borna on BoDV-1 replication. The amount of BoDV-1 gRNA in 293T/BoDV cells was determined by RT-qPCR analyses. (G) Protocol for long-term TD-Borna treatment in 293T/BoDV cells. 293T/BoDV cells were seeded in 12-well plates (2 × 10 5 cells/well) on Day –1. TD-Borna was transfected on Days 0, 6, and 12. The cells were passaged into new 12-well plates (2 × 10 5 cells/well) every 3 days. (H) Effects of long-term TD-Borna treatment on BoDV-1 replication. The amount of BoDV-1 gRNA in 293T/BoDV cells was determined by RT-qPCR analyses. Mock, the scrambled siRNA-treated control. Values are expressed as the mean ± SE of three independent experiments. ∗∗ P

    Journal: Frontiers in Microbiology

    Article Title: A Small Interfering RNA Cocktail Targeting the Nucleoprotein and Large Protein Genes Suppresses Borna Disease Virus Infection

    doi: 10.3389/fmicb.2019.02781

    Figure Lengend Snippet: Reduction in the BoDV-1 load by siRNAs targeting N and M/G/L mRNAs. (A,B) Effects of siRNAs targeting BoDV-1 mRNAs on BoDV-1 mRNA amounts. 293T/BoDV cells were treated with the indicated siRNA for 2 days. The amounts of N (A) and M/G/L (B) mRNAs in 293T/BoDV cells were determined by RT-qPCR analyses. (C) Effects of siRNAs targeting BoDV-1 mRNAs on BoDV-1 replication. The amount of BoDV-1 gRNA in 293T/BoDV cells was determined by RT-qPCR analyses. (D,E) Effects of the siRNA cocktail (TD-Borna) targeting BoDV-1 N and M/G/L mRNAs on BoDV-1 mRNAs. 293T/BoDV cells were treated with TD-Borna for 2 days. The amounts of N (D) and M/G/L (E) mRNAs in 293T/BoDV cells were determined by RT-qPCR analyses. (F) Effects of TD-Borna on BoDV-1 replication. The amount of BoDV-1 gRNA in 293T/BoDV cells was determined by RT-qPCR analyses. (G) Protocol for long-term TD-Borna treatment in 293T/BoDV cells. 293T/BoDV cells were seeded in 12-well plates (2 × 10 5 cells/well) on Day –1. TD-Borna was transfected on Days 0, 6, and 12. The cells were passaged into new 12-well plates (2 × 10 5 cells/well) every 3 days. (H) Effects of long-term TD-Borna treatment on BoDV-1 replication. The amount of BoDV-1 gRNA in 293T/BoDV cells was determined by RT-qPCR analyses. Mock, the scrambled siRNA-treated control. Values are expressed as the mean ± SE of three independent experiments. ∗∗ P

    Article Snippet: RT-qPCR assays for BoDV-1 L mRNA and BoDV-1 gRNA were carried out using Luna Universal Probe qPCR Master Mix (New England Biolabs, Ipswich, United States) and the BoDV-1-specific primers and probe.

    Techniques: Quantitative RT-PCR, Transfection

    Reduction in the BoDV-1 load by the combined use of T-705 and TD-Borna. (A) Protocol for long-term treatment using T-705 and TD-Borna in 293T/BoDV cells. 293T/BoDV cells were seeded in 12-well plates (2 × 10 5 cells/well) on Day –1. TD-Borna was transfected on Days 0 and 6. On Day 0, T-705 was added at 1 h after the transfection. The cells were passaged into new 12-well plates (2 × 10 5 cells/well) with or without T-705 on Days 2, 5, and 8. (B) Effects of long-term treatment with T-705 and TD-Borna on BoDV-1 replication. The amount of BoDV-1 gRNA in 293T/BoDV cells was determined by RT-qPCR analyses. (C) The amount of the N protein in 293T/BoDV cells treated with T-705 and TD-Borna. 293T/BoDV cells were treated as indicated in panel (A) . After 5 days of treatment, the amount of the N protein was determined by western blotting using anti-N and anti-tubulin antibodies. (D) Quantification of the amount of the N protein in panel (C) . The band intensity of the N protein in each sample was normalized with that of tubulin. Mock, the scrambled siRNA-treated control. Values are expressed as the mean ± SE of three independent experiments. ∗ P

    Journal: Frontiers in Microbiology

    Article Title: A Small Interfering RNA Cocktail Targeting the Nucleoprotein and Large Protein Genes Suppresses Borna Disease Virus Infection

    doi: 10.3389/fmicb.2019.02781

    Figure Lengend Snippet: Reduction in the BoDV-1 load by the combined use of T-705 and TD-Borna. (A) Protocol for long-term treatment using T-705 and TD-Borna in 293T/BoDV cells. 293T/BoDV cells were seeded in 12-well plates (2 × 10 5 cells/well) on Day –1. TD-Borna was transfected on Days 0 and 6. On Day 0, T-705 was added at 1 h after the transfection. The cells were passaged into new 12-well plates (2 × 10 5 cells/well) with or without T-705 on Days 2, 5, and 8. (B) Effects of long-term treatment with T-705 and TD-Borna on BoDV-1 replication. The amount of BoDV-1 gRNA in 293T/BoDV cells was determined by RT-qPCR analyses. (C) The amount of the N protein in 293T/BoDV cells treated with T-705 and TD-Borna. 293T/BoDV cells were treated as indicated in panel (A) . After 5 days of treatment, the amount of the N protein was determined by western blotting using anti-N and anti-tubulin antibodies. (D) Quantification of the amount of the N protein in panel (C) . The band intensity of the N protein in each sample was normalized with that of tubulin. Mock, the scrambled siRNA-treated control. Values are expressed as the mean ± SE of three independent experiments. ∗ P

    Article Snippet: RT-qPCR assays for BoDV-1 L mRNA and BoDV-1 gRNA were carried out using Luna Universal Probe qPCR Master Mix (New England Biolabs, Ipswich, United States) and the BoDV-1-specific primers and probe.

    Techniques: Transfection, Quantitative RT-PCR, Western Blot

    Reduction in the BoDV-1 load by TD-Borna in neuronal and non-neuronal cell lines. (A,B) Effect of T-705 on BoDV-1 replication in 293T/BoDV (A) and SH-SY5Y/BoDV (B) cells. (C,D) Effect of TD-Borna on BoDV-1 replication in 293T/BoDV (C) and SH-SY5Y/BoDV (D) cells. The cells were treated with T-705 (A,B) or TD-Borna (C,D) for 2 days. The amount of BoDV-1 gRNA in the cells was determined by RT-qPCR analyses. Mock, the scrambled siRNA-treated control. Values are expressed as the mean ± SE of three independent experiments. **** P

    Journal: Frontiers in Microbiology

    Article Title: A Small Interfering RNA Cocktail Targeting the Nucleoprotein and Large Protein Genes Suppresses Borna Disease Virus Infection

    doi: 10.3389/fmicb.2019.02781

    Figure Lengend Snippet: Reduction in the BoDV-1 load by TD-Borna in neuronal and non-neuronal cell lines. (A,B) Effect of T-705 on BoDV-1 replication in 293T/BoDV (A) and SH-SY5Y/BoDV (B) cells. (C,D) Effect of TD-Borna on BoDV-1 replication in 293T/BoDV (C) and SH-SY5Y/BoDV (D) cells. The cells were treated with T-705 (A,B) or TD-Borna (C,D) for 2 days. The amount of BoDV-1 gRNA in the cells was determined by RT-qPCR analyses. Mock, the scrambled siRNA-treated control. Values are expressed as the mean ± SE of three independent experiments. **** P

    Article Snippet: RT-qPCR assays for BoDV-1 L mRNA and BoDV-1 gRNA were carried out using Luna Universal Probe qPCR Master Mix (New England Biolabs, Ipswich, United States) and the BoDV-1-specific primers and probe.

    Techniques: Quantitative RT-PCR

    Analytical performance of PlasQ ( a ) and PlasID ( b ) assay. a Correlation of P. falciparum standards and the Cq values for both targets, Pspp18S (black circles) and PfvarATS (white circles) of the PlasQ assay. Based on these quadruple replicates of the WHO standards, LODs and qPCR efficiencies were calculated. b The ability of the PlasID assay identifying five different malaria species is shown by a representative, composite amplification plot. DNA from the six Plasmodium species were analysed in separate tubes during the same qPCR experiment

    Journal: Malaria Journal

    Article Title: Molecular monitoring of the diversity of human pathogenic malaria species in blood donations on Bioko Island, Equatorial Guinea

    doi: 10.1186/s12936-019-2639-8

    Figure Lengend Snippet: Analytical performance of PlasQ ( a ) and PlasID ( b ) assay. a Correlation of P. falciparum standards and the Cq values for both targets, Pspp18S (black circles) and PfvarATS (white circles) of the PlasQ assay. Based on these quadruple replicates of the WHO standards, LODs and qPCR efficiencies were calculated. b The ability of the PlasID assay identifying five different malaria species is shown by a representative, composite amplification plot. DNA from the six Plasmodium species were analysed in separate tubes during the same qPCR experiment

    Article Snippet: Each reaction contained 2 µL DNA and 8 µL reaction master mix containing 1× Luna Universal Probe qPCR Master Mix (New England Biolabs, Ipswich, USA) and 1× PlasQ Primer Mix (Additional file ).

    Techniques: Real-time Polymerase Chain Reaction, Amplification