recombinant histone 2b (New England Biolabs)


Structured Review
Recombinant Histone 2b, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant histone 2b/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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recombinant histone 2b (New England Biolabs)


Structured Review
Recombinant Histone 2b, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant histone 2b/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
human h2b (New England Biolabs)


Structured Review
Human H2b, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human h2b/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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human recombinant h2b m2505s (New England Biolabs)


Structured Review
Human Recombinant H2b M2505s, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human recombinant h2b m2505s/product/New England Biolabs
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
m2505s (New England Biolabs)


Structured Review
M2505s, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m2505s/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
e coli (New England Biolabs)


Structured Review
E Coli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/e coli/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
m2505s (New England Biolabs)


Structured Review

M2505s, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m2505s/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Induction of broadly reactive influenza antibodies increases susceptibility to autoimmunity"
Article Title: Induction of broadly reactive influenza antibodies increases susceptibility to autoimmunity
Journal: Cell reports
doi: 10.1016/j.celrep.2022.110482

Figure Legend Snippet: KEY RESOURCES TABLE
Techniques Used: Recombinant, Blocking Assay, Binding Assay, Enzyme-linked Immunosorbent Assay, Stripping Membranes, Microarray, Software, Imaging
h2b histone (New England Biolabs)


Structured Review

H2b Histone, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/h2b histone/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Induction of broadly reactive influenza antibodies increases susceptibility to autoimmunity"
Article Title: Induction of broadly reactive influenza antibodies increases susceptibility to autoimmunity
Journal: Cell reports
doi: 10.1016/j.celrep.2022.110482

Figure Legend Snippet: KEY RESOURCES TABLE
Techniques Used: Recombinant, Blocking Assay, Binding Assay, Enzyme-linked Immunosorbent Assay, Stripping Membranes, Microarray, Software, Imaging
histones h2b (New England Biolabs)


Structured Review

Histones H2b, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/histones h2b/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Induction of broadly reactive influenza antibodies increases susceptibility to autoimmunity"
Article Title: Induction of broadly reactive influenza antibodies increases susceptibility to autoimmunity
Journal: Cell reports
doi: 10.1016/j.celrep.2022.110482

Figure Legend Snippet: KEY RESOURCES TABLE
Techniques Used: Recombinant, Blocking Assay, Binding Assay, Enzyme-linked Immunosorbent Assay, Stripping Membranes, Microarray, Software, Imaging
human h2b (New England Biolabs)


Structured Review
Human H2b, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human h2b/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
h2b (New England Biolabs)


Structured Review

H2b, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/h2b/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "COMMD4 functions with the histone H2A-H2B dimer for the timely repair of DNA double-strand breaks"
Article Title: COMMD4 functions with the histone H2A-H2B dimer for the timely repair of DNA double-strand breaks
Journal: Communications Biology
doi: 10.1038/s42003-021-01998-2

Figure Legend Snippet: a Empty vector (FLAG) and COMMD4-FLAG were immunoprecipitated from HeLa cells and the co-eluting proteins were immunoblotted with antibodies shown. b Immunoprecipitation as a , but this time cells were treated with 6 Gy IR. c A direct interaction for COMMD4 and H2B ± recombinant ATM. d A direct interaction between COMMD4 and H2B ± recombinant ATM and the ATM inhibitor (ATMi) KU-55933. e FLAG and FLAG-tagged H2B WT, and S14E mutant were immunoprecipitated from HeLa cells and the co-eluting proteins were immunoblotted with antibodies against COMMD4 and H2B. IgG heavy chain shows the loading. f Direct interaction between COMMD4 and H2B demonstrating the specific binding region within H2B. No binding was seen in the negative control with only the beads and recombinant COMMD4 (beads), while expression of His-tagged COMMD4 (COMMD4, right most lane) is seen. Schematic on the right shows peptide regions within H2B and Supplementary Table shows the peptide sequences. g Direct interaction between RNF40 and H2B demonstrating the specific binding regions within H2B. Expression of recombinant RNF40 is seen in the control lane. h A three dimensional structure (PDB code 2RVQ) of the heterodimer H2A-H2B wherein H2A and H2B are shown in blue and red ribbon representation, respectively. Tails of histones are disordered and in extended conformation. The phosphorylation sites are shown as spheres.
Techniques Used: Plasmid Preparation, Immunoprecipitation, Recombinant, Mutagenesis, Binding Assay, Negative Control, Expressing

Figure Legend Snippet: a Immunoblot showing H2B monoubiquitination in control and COMMD4-depleted cells ±5 μg/ml of Actinomycin D and ±irradiation. b Immunofluorescence showing RNF40 protein levels with 6 Gy IR at 0 and 4 h post-IR treatment in control and COMMD4-depleted cells. Scale bar denotes 5 μm. Fifty cells were quantified per condition. c In vitro ubiquitination assay of H2B ± recombinant COMMD4 or with COMMD4 and without the E2 enzyme. Monoubiquitination and polyubiquitination of H2B is shown. d Direct interaction between COMMD4 and H2A/H2B, demonstrating that COMMD4 preferentially binds H2B. e In vitro direct interaction between H2A, H2B and COMMD4 ± recombinant CK2. H2B was used pull-out the complexes and shows loading. f Same as e , however, COMMD4 was used to pull-out the complexes. g Direct interaction between H2A, H2B and COMMD4, in the absence and presence of purified nucleosomes, ±recombinant ATM and CK2. COMMD4 was used pull-out the complexes and shows the loading.
Techniques Used: Western Blot, Irradiation, Immunofluorescence, In Vitro, Ubiquitin Assay, Recombinant, Purification