human recombinant histones  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Histone H2A Human Recombinant
    Description:
    Histone H2A Human Recombinant 100 ug
    Catalog Number:
    m2502s
    Price:
    82
    Size:
    100 ug
    Category:
    DNA Binding Proteins
    Buy from Supplier


    Structured Review

    New England Biolabs human recombinant histones
    Histone H2A Human Recombinant
    Histone H2A Human Recombinant 100 ug
    https://www.bioz.com/result/human recombinant histones/product/New England Biolabs
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    human recombinant histones - by Bioz Stars, 2020-04
    99/100 stars

    Images

    Related Articles

    Centrifugation:

    Article Title: Mammalian Protein Arginine Methyltransferase 7 (PRMT7) Specifically Targets RXR Sites in Lysine- and Arginine-rich Regions *
    Article Snippet: Recombinant human histone H2A (GenBankTM accession number ), H2B (GenBankTM accession number ), H3.3 (GenBankTM accession number ), and H4 (GenBankTM accession number ) were purchased from New England Biolabs as 1 mg/ml solutions in 20 m m sodium phosphate, 300 m m NaCl, and 1 m m EDTA. .. In some cases, the histone preparations were dialyzed against 10,000 volumes of the reaction buffer (50 m m potassium HEPES, 10 m m NaCl and 1 m m DTT, pH 7.5) or H2 O, at 4 °C for 1 h. Alternatively, the original buffer was exchanged by diluting histones 40-fold in the reaction buffer and then reconcentrated using an Amicon centrifugation filter; this process was repeated twice.

    Expressing:

    Article Title: PRMT5 interacts with the BCL6 oncoprotein and is required for germinal center formation and lymphoma cell survival
    Article Snippet: The GAL4 expression vector, pGBX1 BCL6, pGBX1 BTB, pGBX1 RD2, and pcDNA3 hemagglutinin (HA) PRMT5 plasmids were previously described. .. Recombinant glutathione S -transferase (GST) PRMT5 protein was from Novus Biologicals (Littleton, CO), recombinant BCL6 was from Origene (Rockville, MD), recombinant GST-MEP50 was from Abnova (Walnut, CA), and recombinant human histone H2A and S -adenosyl-methionine (SAM) were from New England Biolabs (Ipswich, MA).

    Autoradiography:

    Article Title: Bub1-Mediated Adaptation of the Spindle Checkpoint
    Article Snippet: The volume was reduced to 50 µL, and the solution was incubated with 100 µM ATP, 0.2 µCi [gamma-32 P]ATP and substrate (10 µg histone H3 [USBiological, Swampscott, MA]; 1 µg histone H2A human, recombinant [New England BioLabs, MA]; 1 µg histone H1 [Upstate Biotechnology, Lake Placid, NY]; 1 µg BUB1_400-700-MBP and BUB1-T566A_400-700-MBP) at 30°C for 20 min. .. The reaction was stopped by adding SDS loading buffer, and the protein were then separated by SDS-PAGE, stained with Coomassie Brilliant Blue, and analyzed by autoradiography.

    Blocking Assay:

    Article Title: Breaching peripheral tolerance promotes the production of HIV-1–neutralizing antibodies
    Article Snippet: Plates were washed three times and incubated with alkaline phosphatase (AP)-conjugated goat anti–mouse isotype-specific (IgM or IgG) detection antibody (Southern Biotech) diluted 1:1,000 in blocking buffer for 1 h at 37°C. .. For Histone H2A- and H2B-reactive IgM ELISAs, plates were coated with 1 µg/ml of human recombinant histone H2A or H2B (New England Biolabs) in PBS pH 7.4.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Breaching peripheral tolerance promotes the production of HIV-1–neutralizing antibodies
    Article Snippet: Paragraph title: ELISA for autoantigen reactive Ig in sera ... For Histone H2A- and H2B-reactive IgM ELISAs, plates were coated with 1 µg/ml of human recombinant histone H2A or H2B (New England Biolabs) in PBS pH 7.4.

    Incubation:

    Article Title: Quantitative Proteomics to Characterize Specific Histone H2A Proteolysis in Chronic Lymphocytic Leukemia and the Myeloid THP-1 Cell Line
    Article Snippet: The Recombinant human H2A (M2502S) was obtained from New England Biolabs (Ipswich, MA, USA) and bovine histone extract (cat. no. 223565) from Roche (Basel, Switzerland). .. To achieve synchronization at the late G1− early S phase, 2 mM thymidine was added over two intervals of 12–16 h, with an incubation in non-thymidine containing RMPI-1640 medium for 8 h in between 50 ng/mL PMA was added to the THP-1 medium for the differentiation of the non-synchronized cells and 1 µM RA for the differentiation of the synchronized cells.

    Article Title: Bub1-Mediated Adaptation of the Spindle Checkpoint
    Article Snippet: .. The volume was reduced to 50 µL, and the solution was incubated with 100 µM ATP, 0.2 µCi [gamma-32 P]ATP and substrate (10 µg histone H3 [USBiological, Swampscott, MA]; 1 µg histone H2A human, recombinant [New England BioLabs, MA]; 1 µg histone H1 [Upstate Biotechnology, Lake Placid, NY]; 1 µg BUB1_400-700-MBP and BUB1-T566A_400-700-MBP) at 30°C for 20 min. .. The reaction was stopped by adding SDS loading buffer, and the protein were then separated by SDS-PAGE, stained with Coomassie Brilliant Blue, and analyzed by autoradiography.

    Article Title: Breaching peripheral tolerance promotes the production of HIV-1–neutralizing antibodies
    Article Snippet: Plates were washed three times and incubated with alkaline phosphatase (AP)-conjugated goat anti–mouse isotype-specific (IgM or IgG) detection antibody (Southern Biotech) diluted 1:1,000 in blocking buffer for 1 h at 37°C. .. For Histone H2A- and H2B-reactive IgM ELISAs, plates were coated with 1 µg/ml of human recombinant histone H2A or H2B (New England Biolabs) in PBS pH 7.4.

    Mass Spectrometry:

    Article Title: Mammalian Protein Arginine Methyltransferase 7 (PRMT7) Specifically Targets RXR Sites in Lysine- and Arginine-rich Regions *
    Article Snippet: Peptides H2B(23-37), H2B(23-37)R29K, H2B(23-37)R31K, H2B(23-37)R33K, H2B(23-37)R29K,R31K, H2B(23-37)R29K,R33K, H2B(23-37)R31K,R33K, H4(1-8), H4(14-22), H4(14-22)R17K, and H4(14-22)R19K were purchased from GenScript as HPLC-purified materials at > 90% purity and verified by mass spectrometry by the vendor. .. Recombinant human histone H2A (GenBankTM accession number ), H2B (GenBankTM accession number ), H3.3 (GenBankTM accession number ), and H4 (GenBankTM accession number ) were purchased from New England Biolabs as 1 mg/ml solutions in 20 m m sodium phosphate, 300 m m NaCl, and 1 m m EDTA.

    Article Title: Histones bundle F-actin filaments and affect actin structure
    Article Snippet: .. Human recombinant H2A histone was bought from New England Bio Labs (Ipswich, MS). ..

    Modification:

    Article Title: Quantitative Proteomics to Characterize Specific Histone H2A Proteolysis in Chronic Lymphocytic Leukemia and the Myeloid THP-1 Cell Line
    Article Snippet: The Recombinant human H2A (M2502S) was obtained from New England Biolabs (Ipswich, MA, USA) and bovine histone extract (cat. no. 223565) from Roche (Basel, Switzerland). .. Raji, Jurkat and HL-60 cells were cultured in Dulbecco’s Modified Eagle Medium and IM-9, U-937 and THP-1 cells in RPMI-1640 medium, both enriched with 2% (w /v ) l -glutamine, 10% (w /v ) FBS and 50 IU/mL penicillin/streptomycin.

    Article Title: PRMT5 interacts with the BCL6 oncoprotein and is required for germinal center formation and lymphoma cell survival
    Article Snippet: 293T cells were maintained in Dulbecco’s modified Eagle medium (Gibco, Grand Island, NY). .. Recombinant glutathione S -transferase (GST) PRMT5 protein was from Novus Biologicals (Littleton, CO), recombinant BCL6 was from Origene (Rockville, MD), recombinant GST-MEP50 was from Abnova (Walnut, CA), and recombinant human histone H2A and S -adenosyl-methionine (SAM) were from New England Biolabs (Ipswich, MA).

    Kinase Assay:

    Article Title: Bub1-Mediated Adaptation of the Spindle Checkpoint
    Article Snippet: Paragraph title: Kinase assay ... The volume was reduced to 50 µL, and the solution was incubated with 100 µM ATP, 0.2 µCi [gamma-32 P]ATP and substrate (10 µg histone H3 [USBiological, Swampscott, MA]; 1 µg histone H2A human, recombinant [New England BioLabs, MA]; 1 µg histone H1 [Upstate Biotechnology, Lake Placid, NY]; 1 µg BUB1_400-700-MBP and BUB1-T566A_400-700-MBP) at 30°C for 20 min.

    High Performance Liquid Chromatography:

    Article Title: Mammalian Protein Arginine Methyltransferase 7 (PRMT7) Specifically Targets RXR Sites in Lysine- and Arginine-rich Regions *
    Article Snippet: Peptides H2B(23-37), H2B(23-37)R29K, H2B(23-37)R31K, H2B(23-37)R33K, H2B(23-37)R29K,R31K, H2B(23-37)R29K,R33K, H2B(23-37)R31K,R33K, H4(1-8), H4(14-22), H4(14-22)R17K, and H4(14-22)R19K were purchased from GenScript as HPLC-purified materials at > 90% purity and verified by mass spectrometry by the vendor. .. Recombinant human histone H2A (GenBankTM accession number ), H2B (GenBankTM accession number ), H3.3 (GenBankTM accession number ), and H4 (GenBankTM accession number ) were purchased from New England Biolabs as 1 mg/ml solutions in 20 m m sodium phosphate, 300 m m NaCl, and 1 m m EDTA.

    Immunoprecipitation:

    Article Title: Bub1-Mediated Adaptation of the Spindle Checkpoint
    Article Snippet: Briefly, myc-tagged Bub1 and Bub1-T566A cells were immunoprecipitated with a myc epitope antibody and the immunoprecipitate was washed thrice with kinase buffer (50 mM Tris-HCl [pH 7.5], 10 mM MgCl2 , and 1% Triton X-100). .. The volume was reduced to 50 µL, and the solution was incubated with 100 µM ATP, 0.2 µCi [gamma-32 P]ATP and substrate (10 µg histone H3 [USBiological, Swampscott, MA]; 1 µg histone H2A human, recombinant [New England BioLabs, MA]; 1 µg histone H1 [Upstate Biotechnology, Lake Placid, NY]; 1 µg BUB1_400-700-MBP and BUB1-T566A_400-700-MBP) at 30°C for 20 min.

    Cell Culture:

    Article Title: Quantitative Proteomics to Characterize Specific Histone H2A Proteolysis in Chronic Lymphocytic Leukemia and the Myeloid THP-1 Cell Line
    Article Snippet: The Recombinant human H2A (M2502S) was obtained from New England Biolabs (Ipswich, MA, USA) and bovine histone extract (cat. no. 223565) from Roche (Basel, Switzerland). .. Raji, Jurkat and HL-60 cells were cultured in Dulbecco’s Modified Eagle Medium and IM-9, U-937 and THP-1 cells in RPMI-1640 medium, both enriched with 2% (w /v ) l -glutamine, 10% (w /v ) FBS and 50 IU/mL penicillin/streptomycin.

    Article Title: PRMT5 interacts with the BCL6 oncoprotein and is required for germinal center formation and lymphoma cell survival
    Article Snippet: All types of cell culture medium were supplemented with 10% fetal bovine serum (unless stated otherwise), 100 µg/mL penicillin, 100 U/mL streptomycin, and 2 mM l -glutamine (Fisher Scientific). .. Recombinant glutathione S -transferase (GST) PRMT5 protein was from Novus Biologicals (Littleton, CO), recombinant BCL6 was from Origene (Rockville, MD), recombinant GST-MEP50 was from Abnova (Walnut, CA), and recombinant human histone H2A and S -adenosyl-methionine (SAM) were from New England Biolabs (Ipswich, MA).

    Generated:

    Article Title: PRMT5 interacts with the BCL6 oncoprotein and is required for germinal center formation and lymphoma cell survival
    Article Snippet: The synthetic Bcl6 reporter, B6BS-TK-Luc, which contains 4 copies of consensus Bcl6 DNA-binding sites, was a gift of Hilda Ye. pcDNA3.1 HA PRMT5(1-320), pCMV-Sport 6 MEP50, pcDNA3.1 V5 BCL6, pcDNA3.1 V5 BTB, pcDNA3.1 V5 RD2, pcDNA3.1 V5 ZF, and pcDNA3.1 V5 PRMT5 G367A/R368A were generated in our laboratory using standard molecular cloning techniques and confirmed by sequencing. .. Recombinant glutathione S -transferase (GST) PRMT5 protein was from Novus Biologicals (Littleton, CO), recombinant BCL6 was from Origene (Rockville, MD), recombinant GST-MEP50 was from Abnova (Walnut, CA), and recombinant human histone H2A and S -adenosyl-methionine (SAM) were from New England Biolabs (Ipswich, MA).

    Protein Concentration:

    Article Title: DNA binding to proteolytically activated TLR9 is sequence-independent and enhanced by DNA curvature
    Article Snippet: .. Binding constants of HMGB1, human H2A–H2B (New England Biolabs) and mTLR9–cECD were determined with Origin 7 (OriginLab) by fitting the data to ( ): where S Tot is the signal (fluorescence intensity or fluorescence anisotropy) of ODN at protein concentration [ E ]T ; S L is the signal of labelled ODN, L ; S EL is the signal of ODN in the plateau region of the binding curve; [ L ]T is the total concentration of ODN added; K d is the dissociation constant. ..

    Sequencing:

    Article Title: PRMT5 interacts with the BCL6 oncoprotein and is required for germinal center formation and lymphoma cell survival
    Article Snippet: The synthetic Bcl6 reporter, B6BS-TK-Luc, which contains 4 copies of consensus Bcl6 DNA-binding sites, was a gift of Hilda Ye. pcDNA3.1 HA PRMT5(1-320), pCMV-Sport 6 MEP50, pcDNA3.1 V5 BCL6, pcDNA3.1 V5 BTB, pcDNA3.1 V5 RD2, pcDNA3.1 V5 ZF, and pcDNA3.1 V5 PRMT5 G367A/R368A were generated in our laboratory using standard molecular cloning techniques and confirmed by sequencing. .. Recombinant glutathione S -transferase (GST) PRMT5 protein was from Novus Biologicals (Littleton, CO), recombinant BCL6 was from Origene (Rockville, MD), recombinant GST-MEP50 was from Abnova (Walnut, CA), and recombinant human histone H2A and S -adenosyl-methionine (SAM) were from New England Biolabs (Ipswich, MA).

    Binding Assay:

    Article Title: DNA binding to proteolytically activated TLR9 is sequence-independent and enhanced by DNA curvature
    Article Snippet: .. Binding constants of HMGB1, human H2A–H2B (New England Biolabs) and mTLR9–cECD were determined with Origin 7 (OriginLab) by fitting the data to ( ): where S Tot is the signal (fluorescence intensity or fluorescence anisotropy) of ODN at protein concentration [ E ]T ; S L is the signal of labelled ODN, L ; S EL is the signal of ODN in the plateau region of the binding curve; [ L ]T is the total concentration of ODN added; K d is the dissociation constant. ..

    Molecular Cloning:

    Article Title: PRMT5 interacts with the BCL6 oncoprotein and is required for germinal center formation and lymphoma cell survival
    Article Snippet: The synthetic Bcl6 reporter, B6BS-TK-Luc, which contains 4 copies of consensus Bcl6 DNA-binding sites, was a gift of Hilda Ye. pcDNA3.1 HA PRMT5(1-320), pCMV-Sport 6 MEP50, pcDNA3.1 V5 BCL6, pcDNA3.1 V5 BTB, pcDNA3.1 V5 RD2, pcDNA3.1 V5 ZF, and pcDNA3.1 V5 PRMT5 G367A/R368A were generated in our laboratory using standard molecular cloning techniques and confirmed by sequencing. .. Recombinant glutathione S -transferase (GST) PRMT5 protein was from Novus Biologicals (Littleton, CO), recombinant BCL6 was from Origene (Rockville, MD), recombinant GST-MEP50 was from Abnova (Walnut, CA), and recombinant human histone H2A and S -adenosyl-methionine (SAM) were from New England Biolabs (Ipswich, MA).

    Fluorescence:

    Article Title: DNA binding to proteolytically activated TLR9 is sequence-independent and enhanced by DNA curvature
    Article Snippet: .. Binding constants of HMGB1, human H2A–H2B (New England Biolabs) and mTLR9–cECD were determined with Origin 7 (OriginLab) by fitting the data to ( ): where S Tot is the signal (fluorescence intensity or fluorescence anisotropy) of ODN at protein concentration [ E ]T ; S L is the signal of labelled ODN, L ; S EL is the signal of ODN in the plateau region of the binding curve; [ L ]T is the total concentration of ODN added; K d is the dissociation constant. ..

    Purification:

    Article Title: PRMT5 interacts with the BCL6 oncoprotein and is required for germinal center formation and lymphoma cell survival
    Article Snippet: Recombinant glutathione S -transferase (GST) PRMT5 protein was from Novus Biologicals (Littleton, CO), recombinant BCL6 was from Origene (Rockville, MD), recombinant GST-MEP50 was from Abnova (Walnut, CA), and recombinant human histone H2A and S -adenosyl-methionine (SAM) were from New England Biolabs (Ipswich, MA). .. His-tagged RD2 protein was purified by using the Takara HisTALON Superflow Cartridge Purification Kit (Mountain View, CA).

    Article Title: Recruitment of ubiquitin-activating enzyme UBA1 to DNA by poly(ADP-ribose) promotes ATR signalling
    Article Snippet: .. Purified histone H2A (#M2502S) was obtained from New England Biolabs. .. Radioactive pADPr binding assay was performed as described by , with some modifications.

    Article Title: Mammalian Protein Arginine Methyltransferase 7 (PRMT7) Specifically Targets RXR Sites in Lysine- and Arginine-rich Regions *
    Article Snippet: GST-GAR was expressed in E. coli BL21 Star (DE3) cells (Invitrogen, C601003) and purified with glutathione-Sepharose 4B beads (Amersham Biosciences) by affinity chromatography as described previously ( ). .. Recombinant human histone H2A (GenBankTM accession number ), H2B (GenBankTM accession number ), H3.3 (GenBankTM accession number ), and H4 (GenBankTM accession number ) were purchased from New England Biolabs as 1 mg/ml solutions in 20 m m sodium phosphate, 300 m m NaCl, and 1 m m EDTA.

    Article Title: Elevated H3K79 homocysteinylation causes abnormal gene expression during neural development and subsequent neural tube defects
    Article Snippet: .. HTL treatment in vitro Purified histones, including H2a (M2502S, NEB), H2b (M2505S, NEB), H3 (M2503S, NEB) and H4 (M2504S, NEB) were selected. ..

    Affinity Chromatography:

    Article Title: Mammalian Protein Arginine Methyltransferase 7 (PRMT7) Specifically Targets RXR Sites in Lysine- and Arginine-rich Regions *
    Article Snippet: GST-GAR was expressed in E. coli BL21 Star (DE3) cells (Invitrogen, C601003) and purified with glutathione-Sepharose 4B beads (Amersham Biosciences) by affinity chromatography as described previously ( ). .. Recombinant human histone H2A (GenBankTM accession number ), H2B (GenBankTM accession number ), H3.3 (GenBankTM accession number ), and H4 (GenBankTM accession number ) were purchased from New England Biolabs as 1 mg/ml solutions in 20 m m sodium phosphate, 300 m m NaCl, and 1 m m EDTA.

    SDS Page:

    Article Title: Bub1-Mediated Adaptation of the Spindle Checkpoint
    Article Snippet: The volume was reduced to 50 µL, and the solution was incubated with 100 µM ATP, 0.2 µCi [gamma-32 P]ATP and substrate (10 µg histone H3 [USBiological, Swampscott, MA]; 1 µg histone H2A human, recombinant [New England BioLabs, MA]; 1 µg histone H1 [Upstate Biotechnology, Lake Placid, NY]; 1 µg BUB1_400-700-MBP and BUB1-T566A_400-700-MBP) at 30°C for 20 min. .. The reaction was stopped by adding SDS loading buffer, and the protein were then separated by SDS-PAGE, stained with Coomassie Brilliant Blue, and analyzed by autoradiography.

    Plasmid Preparation:

    Article Title: PRMT5 interacts with the BCL6 oncoprotein and is required for germinal center formation and lymphoma cell survival
    Article Snippet: The GAL4 expression vector, pGBX1 BCL6, pGBX1 BTB, pGBX1 RD2, and pcDNA3 hemagglutinin (HA) PRMT5 plasmids were previously described. .. Recombinant glutathione S -transferase (GST) PRMT5 protein was from Novus Biologicals (Littleton, CO), recombinant BCL6 was from Origene (Rockville, MD), recombinant GST-MEP50 was from Abnova (Walnut, CA), and recombinant human histone H2A and S -adenosyl-methionine (SAM) were from New England Biolabs (Ipswich, MA).

    Recombinant:

    Article Title: Quantitative Proteomics to Characterize Specific Histone H2A Proteolysis in Chronic Lymphocytic Leukemia and the Myeloid THP-1 Cell Line
    Article Snippet: .. The Recombinant human H2A (M2502S) was obtained from New England Biolabs (Ipswich, MA, USA) and bovine histone extract (cat. no. 223565) from Roche (Basel, Switzerland). ..

    Article Title: PRMT5 interacts with the BCL6 oncoprotein and is required for germinal center formation and lymphoma cell survival
    Article Snippet: .. Recombinant glutathione S -transferase (GST) PRMT5 protein was from Novus Biologicals (Littleton, CO), recombinant BCL6 was from Origene (Rockville, MD), recombinant GST-MEP50 was from Abnova (Walnut, CA), and recombinant human histone H2A and S -adenosyl-methionine (SAM) were from New England Biolabs (Ipswich, MA). .. His-tagged RD2 protein was purified by using the Takara HisTALON Superflow Cartridge Purification Kit (Mountain View, CA).

    Article Title: Bub1-Mediated Adaptation of the Spindle Checkpoint
    Article Snippet: .. The volume was reduced to 50 µL, and the solution was incubated with 100 µM ATP, 0.2 µCi [gamma-32 P]ATP and substrate (10 µg histone H3 [USBiological, Swampscott, MA]; 1 µg histone H2A human, recombinant [New England BioLabs, MA]; 1 µg histone H1 [Upstate Biotechnology, Lake Placid, NY]; 1 µg BUB1_400-700-MBP and BUB1-T566A_400-700-MBP) at 30°C for 20 min. .. The reaction was stopped by adding SDS loading buffer, and the protein were then separated by SDS-PAGE, stained with Coomassie Brilliant Blue, and analyzed by autoradiography.

    Article Title: Differential Microbicidal Effects of Human Histone Proteins H2A and H2B on Leishmania Promastigotes and Amastigotes ▿
    Article Snippet: .. Recombinant human histone H2A (GenBank accession number ), H2B (GenBank accession number ), and H10 (GenBank accession number ) were purchased from New England BioLabs (Ipswich, MA). ..

    Article Title: Mammalian Protein Arginine Methyltransferase 7 (PRMT7) Specifically Targets RXR Sites in Lysine- and Arginine-rich Regions *
    Article Snippet: .. Recombinant human histone H2A (GenBankTM accession number ), H2B (GenBankTM accession number ), H3.3 (GenBankTM accession number ), and H4 (GenBankTM accession number ) were purchased from New England Biolabs as 1 mg/ml solutions in 20 m m sodium phosphate, 300 m m NaCl, and 1 m m EDTA. ..

    Article Title: Breaching peripheral tolerance promotes the production of HIV-1–neutralizing antibodies
    Article Snippet: .. For Histone H2A- and H2B-reactive IgM ELISAs, plates were coated with 1 µg/ml of human recombinant histone H2A or H2B (New England Biolabs) in PBS pH 7.4. ..

    Article Title: Histones bundle F-actin filaments and affect actin structure
    Article Snippet: .. Human recombinant H2A histone was bought from New England Bio Labs (Ipswich, MS). ..

    Article Title: Proteolytic Histone Modification by Mast Cell Tryptase, a Serglycin Proteoglycan-dependent Secretory Granule Protease *
    Article Snippet: .. Recombinant human histone H2A, H2B, H3.1, and H4 were from New England Biolabs (Ipswich, MA). .. LysoTracker Red DND-99 was from Life Technologies.

    In Vitro:

    Article Title: Elevated H3K79 homocysteinylation causes abnormal gene expression during neural development and subsequent neural tube defects
    Article Snippet: .. HTL treatment in vitro Purified histones, including H2a (M2502S, NEB), H2b (M2505S, NEB), H3 (M2503S, NEB) and H4 (M2504S, NEB) were selected. ..

    Concentration Assay:

    Article Title: DNA binding to proteolytically activated TLR9 is sequence-independent and enhanced by DNA curvature
    Article Snippet: .. Binding constants of HMGB1, human H2A–H2B (New England Biolabs) and mTLR9–cECD were determined with Origin 7 (OriginLab) by fitting the data to ( ): where S Tot is the signal (fluorescence intensity or fluorescence anisotropy) of ODN at protein concentration [ E ]T ; S L is the signal of labelled ODN, L ; S EL is the signal of ODN in the plateau region of the binding curve; [ L ]T is the total concentration of ODN added; K d is the dissociation constant. ..

    Staining:

    Article Title: Bub1-Mediated Adaptation of the Spindle Checkpoint
    Article Snippet: The volume was reduced to 50 µL, and the solution was incubated with 100 µM ATP, 0.2 µCi [gamma-32 P]ATP and substrate (10 µg histone H3 [USBiological, Swampscott, MA]; 1 µg histone H2A human, recombinant [New England BioLabs, MA]; 1 µg histone H1 [Upstate Biotechnology, Lake Placid, NY]; 1 µg BUB1_400-700-MBP and BUB1-T566A_400-700-MBP) at 30°C for 20 min. .. The reaction was stopped by adding SDS loading buffer, and the protein were then separated by SDS-PAGE, stained with Coomassie Brilliant Blue, and analyzed by autoradiography.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    New England Biolabs recombinant human histone h3 1 protein
    Western blot analysis of histone <t>H3.1</t> proteins; 1 μg methylated Xenopus histone H3.1 (Lane 1), 10 μg methylated human histone H3.1 (Lane 2), 0.5 μg methylated human histone H3.1 (Lane 3), 2 μg methylated human histone H3.1(Lane 4), 1 μg Xenopus histone H3.1 (Lane 5), 10 μg Human histone 3.1 (Lane 6), 0.5 μg human histone H3.1 (Lane 7), and 2 μg human histone H3.1 (Lane 8).
    Recombinant Human Histone H3 1 Protein, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant human histone h3 1 protein/product/New England Biolabs
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    recombinant human histone h3 1 protein - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    Western blot analysis of histone H3.1 proteins; 1 μg methylated Xenopus histone H3.1 (Lane 1), 10 μg methylated human histone H3.1 (Lane 2), 0.5 μg methylated human histone H3.1 (Lane 3), 2 μg methylated human histone H3.1(Lane 4), 1 μg Xenopus histone H3.1 (Lane 5), 10 μg Human histone 3.1 (Lane 6), 0.5 μg human histone H3.1 (Lane 7), and 2 μg human histone H3.1 (Lane 8).

    Journal: Anticancer research

    Article Title: Histone Methylation by Temozolomide; A Classic DNA Methylating Anticancer Drug

    doi:

    Figure Lengend Snippet: Western blot analysis of histone H3.1 proteins; 1 μg methylated Xenopus histone H3.1 (Lane 1), 10 μg methylated human histone H3.1 (Lane 2), 0.5 μg methylated human histone H3.1 (Lane 3), 2 μg methylated human histone H3.1(Lane 4), 1 μg Xenopus histone H3.1 (Lane 5), 10 μg Human histone 3.1 (Lane 6), 0.5 μg human histone H3.1 (Lane 7), and 2 μg human histone H3.1 (Lane 8).

    Article Snippet: Recombinant human histone H3.1 protein (MW=15273.2 Da by ESI-TOF) was purchased from New England BioLabs, Inc. (Ipswich, MA, USA).

    Techniques: Western Blot, Methylation

    The N-, C-, and the linker domains in human HCS interact with histone H3.2 in yeast-two-hybrid assays (A) plate layout of HCS interactions; the interaction between p53 and T antigen was used as positive control; (B) activation of reporter genes and secretion of a-galactosidase, mediated by HCS-H3 interactions (arrows); and (C) successful co-transformation of test plasmids was verified by growing AH109 host strain on SD/−Leu, −Trp, +Kan plates;.

    Journal: The Journal of nutritional biochemistry

    Article Title: Holocarboxylase synthetase is a chromatin protein and interacts directly with histone H3 to mediate biotinylation of K9 and K18

    doi: 10.1016/j.jnutbio.2010.04.001

    Figure Lengend Snippet: The N-, C-, and the linker domains in human HCS interact with histone H3.2 in yeast-two-hybrid assays (A) plate layout of HCS interactions; the interaction between p53 and T antigen was used as positive control; (B) activation of reporter genes and secretion of a-galactosidase, mediated by HCS-H3 interactions (arrows); and (C) successful co-transformation of test plasmids was verified by growing AH109 host strain on SD/−Leu, −Trp, +Kan plates;.

    Article Snippet: Input control and recombinant human histone H3.2 (NewEngland Biolabs, Ipswich, MA) were used as positive controls, whereas the sample precipitated with non-specific IgG was used as negative control.

    Techniques: Positive Control, Activation Assay, Transformation Assay

    HCS interacts physically with histone H3.2 in HEK293 cells Panel A: Nuclear extracts from HEK293 HCS-GFP cells were precipitated with anti-GFP and probed with anti-histone H3. Nuclear extracts precipitated with non-specific IgG was used as negative control. IgG precipitates (without anti-GFP) were probed with anti-histone H3 (“IgG”); Nuclear extracts without antibody treatment (input), and 0.1 μg of recombinant human histone H3.2 (rH3.2) were used as positive controls. Panel B: Nuclear extracts from HEK293 cells were precipitated with anti-HCS and probed with anti-histone H3 (“HCS pulldown”). As positive control, nuclear extracts collected before treatment with anti-HCS were probed with anti-histone H3 (“Input”); as negative control, protein A precipitates (without anti-HCS) were probed with anti-histone H3 (“Protein A”). Panel C: Purified rHCS was probed with anti-human HCS (lane 1), anti-poly·his tag (lane 2), and coomassie blue (lane 3). Panel D: rHCS was incubated with p67 and cofactors for enzymatic biotinylation; negative controls were generated by omission of individual compounds from reaction mixtures. p67-bound biotin was probed using streptavidin. Panel E: Preincubation of H3 with HCS protects H3 against proteolysis by trypsin in limited proteolysis assays. Left = recombinant histone H3.2 alone; middle = H3.2 pre-incubated with HCS-GST; right = H3.2 pre-incubated with GST alone. H3 was probed with coomassie blue (top panel) and anti-histone H3.2 (bottom panel).

    Journal: The Journal of nutritional biochemistry

    Article Title: Holocarboxylase synthetase is a chromatin protein and interacts directly with histone H3 to mediate biotinylation of K9 and K18

    doi: 10.1016/j.jnutbio.2010.04.001

    Figure Lengend Snippet: HCS interacts physically with histone H3.2 in HEK293 cells Panel A: Nuclear extracts from HEK293 HCS-GFP cells were precipitated with anti-GFP and probed with anti-histone H3. Nuclear extracts precipitated with non-specific IgG was used as negative control. IgG precipitates (without anti-GFP) were probed with anti-histone H3 (“IgG”); Nuclear extracts without antibody treatment (input), and 0.1 μg of recombinant human histone H3.2 (rH3.2) were used as positive controls. Panel B: Nuclear extracts from HEK293 cells were precipitated with anti-HCS and probed with anti-histone H3 (“HCS pulldown”). As positive control, nuclear extracts collected before treatment with anti-HCS were probed with anti-histone H3 (“Input”); as negative control, protein A precipitates (without anti-HCS) were probed with anti-histone H3 (“Protein A”). Panel C: Purified rHCS was probed with anti-human HCS (lane 1), anti-poly·his tag (lane 2), and coomassie blue (lane 3). Panel D: rHCS was incubated with p67 and cofactors for enzymatic biotinylation; negative controls were generated by omission of individual compounds from reaction mixtures. p67-bound biotin was probed using streptavidin. Panel E: Preincubation of H3 with HCS protects H3 against proteolysis by trypsin in limited proteolysis assays. Left = recombinant histone H3.2 alone; middle = H3.2 pre-incubated with HCS-GST; right = H3.2 pre-incubated with GST alone. H3 was probed with coomassie blue (top panel) and anti-histone H3.2 (bottom panel).

    Article Snippet: Input control and recombinant human histone H3.2 (NewEngland Biolabs, Ipswich, MA) were used as positive controls, whereas the sample precipitated with non-specific IgG was used as negative control.

    Techniques: Negative Control, Recombinant, Positive Control, Purification, Incubation, Generated

    Recombinant human HCS catalyzes biotinylation of histone H3.2 Panel A: rHCS was incubated with recombinant human histone H3.2 and cofactors for enzymatic biotinylation for up to 12 h; negative controls were generated by omission of rHCS and H3.2. Samples were collected at timed intervals and histone-bound biotin was probed using anti-biotin. Equal loading of histone H3.2 was confirmed by staining with Coomassie blue. Panel B: rHCS was incubated with recombinant human histone H3.2 and cofactors for enzymatic biotinylation for 12 h; negative controls were generated by omission of rHCS and H3.2. Histone-bound biotin was probed using streptavidin. Staining with Coomassie blue was used as loading control. Panel C: Same as panel B, but a synthetic peptide representing amino acids 1 to 25 (N 1–25 ) in human histone H3.2 was used as substrate. Negative controls were generated by omission of rHCS and peptide.

    Journal: The Journal of nutritional biochemistry

    Article Title: Holocarboxylase synthetase is a chromatin protein and interacts directly with histone H3 to mediate biotinylation of K9 and K18

    doi: 10.1016/j.jnutbio.2010.04.001

    Figure Lengend Snippet: Recombinant human HCS catalyzes biotinylation of histone H3.2 Panel A: rHCS was incubated with recombinant human histone H3.2 and cofactors for enzymatic biotinylation for up to 12 h; negative controls were generated by omission of rHCS and H3.2. Samples were collected at timed intervals and histone-bound biotin was probed using anti-biotin. Equal loading of histone H3.2 was confirmed by staining with Coomassie blue. Panel B: rHCS was incubated with recombinant human histone H3.2 and cofactors for enzymatic biotinylation for 12 h; negative controls were generated by omission of rHCS and H3.2. Histone-bound biotin was probed using streptavidin. Staining with Coomassie blue was used as loading control. Panel C: Same as panel B, but a synthetic peptide representing amino acids 1 to 25 (N 1–25 ) in human histone H3.2 was used as substrate. Negative controls were generated by omission of rHCS and peptide.

    Article Snippet: Input control and recombinant human histone H3.2 (NewEngland Biolabs, Ipswich, MA) were used as positive controls, whereas the sample precipitated with non-specific IgG was used as negative control.

    Techniques: Recombinant, Incubation, Generated, Staining