et ssb  (New England Biolabs)


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    New England Biolabs et ssb
    (a) Effect of <t>gp32</t> protein on the ttp for HIV-1 using NASBA and HIV-1 primer set ( S1 File ) (n = 3). (a) Optimization of gp32 using 0, 3, 4.5, 7.2 and 9.6 μM (0, 100, 150, 240, 320 ng/μL) gp32. Sample contained ~1 x 10 5 copies of synthetic HIV-1 RNA; conventional 2-step NASBA included as reference, NTCs as 2-step (no gp32) and 1-step (with 4.5 μM gp32) (b) Comparison of NASBA carried out as conventional 2-step, one step in absence of <t>SSB,</t> and 1-step using 3 μM gp32 for HIV-1 RNA samples ranging from C t 20 (~1.5 x 10 6 copies) to C t 34 (~1.5 x 10 2 copies).
    Et Ssb, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/et ssb/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    et ssb - by Bioz Stars, 2022-05
    94/100 stars

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    1) Product Images from "An improved nucleic acid sequence-based amplification method mediated by T4 gene 32 protein"

    Article Title: An improved nucleic acid sequence-based amplification method mediated by T4 gene 32 protein

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0265391

    (a) Effect of gp32 protein on the ttp for HIV-1 using NASBA and HIV-1 primer set ( S1 File ) (n = 3). (a) Optimization of gp32 using 0, 3, 4.5, 7.2 and 9.6 μM (0, 100, 150, 240, 320 ng/μL) gp32. Sample contained ~1 x 10 5 copies of synthetic HIV-1 RNA; conventional 2-step NASBA included as reference, NTCs as 2-step (no gp32) and 1-step (with 4.5 μM gp32) (b) Comparison of NASBA carried out as conventional 2-step, one step in absence of SSB, and 1-step using 3 μM gp32 for HIV-1 RNA samples ranging from C t 20 (~1.5 x 10 6 copies) to C t 34 (~1.5 x 10 2 copies).
    Figure Legend Snippet: (a) Effect of gp32 protein on the ttp for HIV-1 using NASBA and HIV-1 primer set ( S1 File ) (n = 3). (a) Optimization of gp32 using 0, 3, 4.5, 7.2 and 9.6 μM (0, 100, 150, 240, 320 ng/μL) gp32. Sample contained ~1 x 10 5 copies of synthetic HIV-1 RNA; conventional 2-step NASBA included as reference, NTCs as 2-step (no gp32) and 1-step (with 4.5 μM gp32) (b) Comparison of NASBA carried out as conventional 2-step, one step in absence of SSB, and 1-step using 3 μM gp32 for HIV-1 RNA samples ranging from C t 20 (~1.5 x 10 6 copies) to C t 34 (~1.5 x 10 2 copies).

    Techniques Used:

    The effect of SSBs on NASBA of the synthetic HIV-1 RNA gene using (a) 80 μg ET SSB, (b) 2 μg RecA and (c) 1 μg gp32 of a sample containing 2 x 10 5 copies of synthetic HIV-1 RNA (~C t 23) using the HIV-1 NASBA primer-probe set ( S1 File ). All graphs show conventional 2-step NASBA (red), 1-step NASBA (black dash/dot), 1-step NASBA with SSB (blue dashed), and NTC in absence (solid black) and presence (solid blue) of SSB (n = 3).
    Figure Legend Snippet: The effect of SSBs on NASBA of the synthetic HIV-1 RNA gene using (a) 80 μg ET SSB, (b) 2 μg RecA and (c) 1 μg gp32 of a sample containing 2 x 10 5 copies of synthetic HIV-1 RNA (~C t 23) using the HIV-1 NASBA primer-probe set ( S1 File ). All graphs show conventional 2-step NASBA (red), 1-step NASBA (black dash/dot), 1-step NASBA with SSB (blue dashed), and NTC in absence (solid black) and presence (solid blue) of SSB (n = 3).

    Techniques Used:

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    New England Biolabs et ssb
    (a) Effect of <t>gp32</t> protein on the ttp for HIV-1 using NASBA and HIV-1 primer set ( S1 File ) (n = 3). (a) Optimization of gp32 using 0, 3, 4.5, 7.2 and 9.6 μM (0, 100, 150, 240, 320 ng/μL) gp32. Sample contained ~1 x 10 5 copies of synthetic HIV-1 RNA; conventional 2-step NASBA included as reference, NTCs as 2-step (no gp32) and 1-step (with 4.5 μM gp32) (b) Comparison of NASBA carried out as conventional 2-step, one step in absence of <t>SSB,</t> and 1-step using 3 μM gp32 for HIV-1 RNA samples ranging from C t 20 (~1.5 x 10 6 copies) to C t 34 (~1.5 x 10 2 copies).
    Et Ssb, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/et ssb/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    et ssb - by Bioz Stars, 2022-05
    94/100 stars
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    (a) Effect of gp32 protein on the ttp for HIV-1 using NASBA and HIV-1 primer set ( S1 File ) (n = 3). (a) Optimization of gp32 using 0, 3, 4.5, 7.2 and 9.6 μM (0, 100, 150, 240, 320 ng/μL) gp32. Sample contained ~1 x 10 5 copies of synthetic HIV-1 RNA; conventional 2-step NASBA included as reference, NTCs as 2-step (no gp32) and 1-step (with 4.5 μM gp32) (b) Comparison of NASBA carried out as conventional 2-step, one step in absence of SSB, and 1-step using 3 μM gp32 for HIV-1 RNA samples ranging from C t 20 (~1.5 x 10 6 copies) to C t 34 (~1.5 x 10 2 copies).

    Journal: PLoS ONE

    Article Title: An improved nucleic acid sequence-based amplification method mediated by T4 gene 32 protein

    doi: 10.1371/journal.pone.0265391

    Figure Lengend Snippet: (a) Effect of gp32 protein on the ttp for HIV-1 using NASBA and HIV-1 primer set ( S1 File ) (n = 3). (a) Optimization of gp32 using 0, 3, 4.5, 7.2 and 9.6 μM (0, 100, 150, 240, 320 ng/μL) gp32. Sample contained ~1 x 10 5 copies of synthetic HIV-1 RNA; conventional 2-step NASBA included as reference, NTCs as 2-step (no gp32) and 1-step (with 4.5 μM gp32) (b) Comparison of NASBA carried out as conventional 2-step, one step in absence of SSB, and 1-step using 3 μM gp32 for HIV-1 RNA samples ranging from C t 20 (~1.5 x 10 6 copies) to C t 34 (~1.5 x 10 2 copies).

    Article Snippet: In SSB mediated NASBA, SSBs were added to the amplification mix in combination with the LEM cocktail at 2 μg of RecA (M0249, New England Biolabs (NEB)), 80 ng ET SSB (M2401S, NEB) or gp32 (M0300, NEB) at a concentration range between 50–320 ng/μL per reaction respectively.

    Techniques:

    The effect of SSBs on NASBA of the synthetic HIV-1 RNA gene using (a) 80 μg ET SSB, (b) 2 μg RecA and (c) 1 μg gp32 of a sample containing 2 x 10 5 copies of synthetic HIV-1 RNA (~C t 23) using the HIV-1 NASBA primer-probe set ( S1 File ). All graphs show conventional 2-step NASBA (red), 1-step NASBA (black dash/dot), 1-step NASBA with SSB (blue dashed), and NTC in absence (solid black) and presence (solid blue) of SSB (n = 3).

    Journal: PLoS ONE

    Article Title: An improved nucleic acid sequence-based amplification method mediated by T4 gene 32 protein

    doi: 10.1371/journal.pone.0265391

    Figure Lengend Snippet: The effect of SSBs on NASBA of the synthetic HIV-1 RNA gene using (a) 80 μg ET SSB, (b) 2 μg RecA and (c) 1 μg gp32 of a sample containing 2 x 10 5 copies of synthetic HIV-1 RNA (~C t 23) using the HIV-1 NASBA primer-probe set ( S1 File ). All graphs show conventional 2-step NASBA (red), 1-step NASBA (black dash/dot), 1-step NASBA with SSB (blue dashed), and NTC in absence (solid black) and presence (solid blue) of SSB (n = 3).

    Article Snippet: In SSB mediated NASBA, SSBs were added to the amplification mix in combination with the LEM cocktail at 2 μg of RecA (M0249, New England Biolabs (NEB)), 80 ng ET SSB (M2401S, NEB) or gp32 (M0300, NEB) at a concentration range between 50–320 ng/μL per reaction respectively.

    Techniques: