warmstart colorimetric lamp 2x master mix  (New England Biolabs)


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    WarmStart Colorimetric LAMP 2X Master Mix DNA and RNA
    Description:
    WarmStart Colorimetric LAMP 2X Master Mix DNA and RNA 500 rxns
    Catalog Number:
    m1800l
    Price:
    832
    Size:
    500 rxns
    Category:
    Thermostable DNA Polymerases
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    Structured Review

    New England Biolabs warmstart colorimetric lamp 2x master mix
    WarmStart Colorimetric LAMP 2X Master Mix DNA and RNA
    WarmStart Colorimetric LAMP 2X Master Mix DNA and RNA 500 rxns
    https://www.bioz.com/result/warmstart colorimetric lamp 2x master mix/product/New England Biolabs
    Average 95 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    warmstart colorimetric lamp 2x master mix - by Bioz Stars, 2020-02
    95/100 stars

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    fip/bip primers
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    lavalamp master mix lucigen
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    iso-001nd
    optigene
    bip

    Images

    1) Product Images from "Fully 3D Printed Integrated Reactor Array for Point-of-Care Molecular Diagnostics"

    Article Title: Fully 3D Printed Integrated Reactor Array for Point-of-Care Molecular Diagnostics

    Journal: Biosensors & bioelectronics

    doi: 10.1016/j.bios.2018.03.009

    Colorimetric and fluorescence based detection for NAATs in 3D printed reactor array. A) Representative photographs of colorimetric LAMP assay for detection of N. meningitidis with 0, 50, 500 and 5000 CFU/reaction on the same chip, alongside LAMP fluorescence based image at given time interval. B) LAMP amplification curves for P. falciparum with 0, 0.1 1, 10, 100, 1000 pg per reaction. C) Calibration curve for P. falciparum as function of log target concentration, n=3. D) LAMP amplification curves for N. meningitidis with 0, 50, 500, 5000 CFU per reaction. E) Calibration curve for N. meningitidis as function of log target concentration, n=3. WarmStart ® LAMP master mix was used.
    Figure Legend Snippet: Colorimetric and fluorescence based detection for NAATs in 3D printed reactor array. A) Representative photographs of colorimetric LAMP assay for detection of N. meningitidis with 0, 50, 500 and 5000 CFU/reaction on the same chip, alongside LAMP fluorescence based image at given time interval. B) LAMP amplification curves for P. falciparum with 0, 0.1 1, 10, 100, 1000 pg per reaction. C) Calibration curve for P. falciparum as function of log target concentration, n=3. D) LAMP amplification curves for N. meningitidis with 0, 50, 500, 5000 CFU per reaction. E) Calibration curve for N. meningitidis as function of log target concentration, n=3. WarmStart ® LAMP master mix was used.

    Techniques Used: Fluorescence, Lamp Assay, Chromatin Immunoprecipitation, Amplification, Concentration Assay

    Related Articles

    Colorimetric Assay:

    Article Title: Simpler, Faster, and Sensitive Zika Virus Assay Using Smartphone Detection of Loop-mediated Isothermal Amplification on Paper Microfluidic Chips
    Article Snippet: .. For this purpose, pH indicator-based colorimetric assay kits could be used (e.g., WarmStart colorimetric LAMP master mix by New England Biolabs), which includes a pH indicator (phenol red) in the master mix reagent, requiring no additional step, no excitation light source, and no closed container (identifiable under ambient light) . ..

    Negative Control:

    Article Title: Comparative analysis of different molecular and serological methods for detection of Xylella fastidiosa in blueberry
    Article Snippet: For LavaLAMP™ master mix (Lucigen), the primer mixture was comprised of 2 μM of each F3/B3, 8 μM of each LF/LB and 16 μM of each FIP/BIP primers, and for WarmStart® Colorimetric 2X Master Mix (NEB), 2 μM of each F3/B3, 4 μM of each LF/LB and 16 μM of each FIP/BIP primers were used ( ). .. Each run contained DNA extract from pure X . fastidiosa culture as positive control and PCR grade water as negative control instead of DNA.

    Amplification:

    Article Title: Comparative analysis of different molecular and serological methods for detection of Xylella fastidiosa in blueberry
    Article Snippet: LAMP assay To detect the pathogen using LAMP amplification, LavaLAMP™ DNA Master Mix (Lucigen, WI, USA) and WarmStart® Colorimetric LAMP 2X Master Mix (New England Bio Labs Ltd., UK) which utilizes pH sensitive phenol red as an end-point indicator, was used according to manufacturer’s instruction. .. For LavaLAMP™ master mix (Lucigen), the primer mixture was comprised of 2 μM of each F3/B3, 8 μM of each LF/LB and 16 μM of each FIP/BIP primers, and for WarmStart® Colorimetric 2X Master Mix (NEB), 2 μM of each F3/B3, 4 μM of each LF/LB and 16 μM of each FIP/BIP primers were used ( ).

    Article Title: Simpler, Faster, and Sensitive Zika Virus Assay Using Smartphone Detection of Loop-mediated Isothermal Amplification on Paper Microfluidic Chips
    Article Snippet: For field applications, a simpler type of dye is needed that is affected by neither ambient lighting perturbations nor the presence of other amplification-inhibiting molecules in the sample. .. For this purpose, pH indicator-based colorimetric assay kits could be used (e.g., WarmStart colorimetric LAMP master mix by New England Biolabs), which includes a pH indicator (phenol red) in the master mix reagent, requiring no additional step, no excitation light source, and no closed container (identifiable under ambient light) .

    Article Title: Comparative analysis of different molecular and serological methods for detection of Xylella fastidiosa in blueberry
    Article Snippet: .. LAMP assay To detect the pathogen using LAMP amplification, LavaLAMP™ DNA Master Mix (Lucigen, WI, USA) and WarmStart® Colorimetric LAMP 2X Master Mix (New England Bio Labs Ltd., UK) which utilizes pH sensitive phenol red as an end-point indicator, was used according to manufacturer’s instruction. .. Each reaction mixture used in this study contained 12.5 μl 2X master mix, 2.5 μl of primer mix, 50 ng of X . fastidiosa experimental DNA samples and 1 μl samples from serially diluted pure culture (previously described in methods) for sensitivity analysis of the assay for X . fastidiosa detection with the rest were filled with DNase/RNase free PCR certified water (TEKNOVA, Hollister, USA) to a final volume of 25 μl.

    Article Title: Comparative analysis of different molecular and serological methods for detection of Xylella fastidiosa in blueberry
    Article Snippet: .. Finally, the amplified end product from WarmStart® Colorimetric LAMP 2X Master Mix (NEB) was assessed visually with the naked eye. .. Double antibody sandwich ELISA (DAS-ELISA) To check the sensitivity of DAS-ELISA (Agdia®, Inc. Elkhart, IN) for X . fastidiosa detection a known concentration of the pathogen was added to the sample buffer in the early stages of the extraction procedure.

    Agarose Gel Electrophoresis:

    Article Title: Comparative analysis of different molecular and serological methods for detection of Xylella fastidiosa in blueberry
    Article Snippet: For LavaLAMP™ master mix (Lucigen), the primer mixture was comprised of 2 μM of each F3/B3, 8 μM of each LF/LB and 16 μM of each FIP/BIP primers, and for WarmStart® Colorimetric 2X Master Mix (NEB), 2 μM of each F3/B3, 4 μM of each LF/LB and 16 μM of each FIP/BIP primers were used ( ). .. LAMP PCR amplification products using LavaLAMP™ master mix (Lucigen) were evaluated by electrophoresis on 1.5% Tris-borate-EDTA (TBE) agarose gel stained with Gelgreen and visualized under ultraviolet (UV) light by Biorad molecular imager Gel Doc™ XR+ with image lab™ software (Biorad, Hercules, CA).

    Fluorescence:

    Article Title: Simpler, Faster, and Sensitive Zika Virus Assay Using Smartphone Detection of Loop-mediated Isothermal Amplification on Paper Microfluidic Chips
    Article Snippet: For this purpose, pH indicator-based colorimetric assay kits could be used (e.g., WarmStart colorimetric LAMP master mix by New England Biolabs), which includes a pH indicator (phenol red) in the master mix reagent, requiring no additional step, no excitation light source, and no closed container (identifiable under ambient light) . .. While smartphone camera detection has been demonstrated for RT-LAMP, only fluorescence detection has been demonstrated , , which requires an additional light source, optical filters, and a closed container.

    Positive Control:

    Article Title: Comparative analysis of different molecular and serological methods for detection of Xylella fastidiosa in blueberry
    Article Snippet: For LavaLAMP™ master mix (Lucigen), the primer mixture was comprised of 2 μM of each F3/B3, 8 μM of each LF/LB and 16 μM of each FIP/BIP primers, and for WarmStart® Colorimetric 2X Master Mix (NEB), 2 μM of each F3/B3, 4 μM of each LF/LB and 16 μM of each FIP/BIP primers were used ( ). .. Each run contained DNA extract from pure X . fastidiosa culture as positive control and PCR grade water as negative control instead of DNA.

    RT Lamp Assay:

    Article Title: A Mismatch-Tolerant Reverse Transcription Loop-Mediated Isothermal Amplification Method and Its Application on Simultaneous Detection of All Four Serotype of Dengue Viruses
    Article Snippet: .. Another visual RT-LAMP assay was performed with the WarmStart Colorimetric LAMP 2X Master Mix (New England Biolabs, Beverly, MA, United States), which uses cresol red as a visual indicator. ..

    Size-exclusion Chromatography:

    Article Title: Establishment ofwMelWolbachia inAedes aegypti mosquitoes and reduction of local dengue transmission in Cairns and surrounding locations in northern Queensland, Australia
    Article Snippet: The qPCR cycling program consisted of a denaturation at 95°C for 5 min followed by 45 cycles of PCR (denaturation at 95 °C for 10 sec, annealing at 60 °C for 15 sec, and extension at 72 °C for 1 sec with single acquisition) followed by a cooling down step at 40°C for 10 sec. From September 2018Wolbachia diagnostics were performed by LAMP. .. Individual reactions consisted of 2X WarmStart® Colorimetric LAMP Master Mix (New England BioLabs, Cat# M1800S), primers and 1 μL of target DNA in a total reaction volume of 17 μL.

    Blocking Assay:

    Article Title: Rapid and simple colorimetric detection of multiple influenza viruses infecting humans using a reverse transcriptional loop-mediated isothermal amplification (RT-LAMP) diagnostic platform
    Article Snippet: For RT-LAMP reactions, a master mix solution was prepared containing 5 μl of WarmStart® Colorimetric LAMP 2X Master Mix (NEB, UK), 1 μl of each F3 and B3 primer, 1 μl of each FIP and BIP primer, and 1 μl of each LF and LB primer for each reaction. .. It was then heated at 80 °C for 10 min for enzyme inactivation in a heat block.

    Electrophoresis:

    Article Title: Comparative analysis of different molecular and serological methods for detection of Xylella fastidiosa in blueberry
    Article Snippet: For LavaLAMP™ master mix (Lucigen), the primer mixture was comprised of 2 μM of each F3/B3, 8 μM of each LF/LB and 16 μM of each FIP/BIP primers, and for WarmStart® Colorimetric 2X Master Mix (NEB), 2 μM of each F3/B3, 4 μM of each LF/LB and 16 μM of each FIP/BIP primers were used ( ). .. LAMP PCR amplification products using LavaLAMP™ master mix (Lucigen) were evaluated by electrophoresis on 1.5% Tris-borate-EDTA (TBE) agarose gel stained with Gelgreen and visualized under ultraviolet (UV) light by Biorad molecular imager Gel Doc™ XR+ with image lab™ software (Biorad, Hercules, CA).

    Polymerase Chain Reaction:

    Article Title: Comparative analysis of different molecular and serological methods for detection of Xylella fastidiosa in blueberry
    Article Snippet: Each reaction mixture used in this study contained 12.5 μl 2X master mix, 2.5 μl of primer mix, 50 ng of X . fastidiosa experimental DNA samples and 1 μl samples from serially diluted pure culture (previously described in methods) for sensitivity analysis of the assay for X . fastidiosa detection with the rest were filled with DNase/RNase free PCR certified water (TEKNOVA, Hollister, USA) to a final volume of 25 μl. .. For LavaLAMP™ master mix (Lucigen), the primer mixture was comprised of 2 μM of each F3/B3, 8 μM of each LF/LB and 16 μM of each FIP/BIP primers, and for WarmStart® Colorimetric 2X Master Mix (NEB), 2 μM of each F3/B3, 4 μM of each LF/LB and 16 μM of each FIP/BIP primers were used ( ).

    Article Title: Rapid and Sensitive Detection of Azole-Resistant Aspergillus fumigatus by Tandem Repeat Loop-Mediated Isothermal Amplification
    Article Snippet: .. LAMP reactions were performed in 0.2-mL PCR tubes in a 25-μL reaction volume, which contains 12.5 μL of WarmStart Colorimetric LAMP 2X master mix, 2.5 μL of TR34-LAMP primer mix (10×), 2.5 μL of gDNA extracted from clinical samples, and 7.5 μL of nuclease-free water. .. All reaction mixes were incubated in an end-point PCR machine (Veriti Dx 96-well Fast Thermal Cycler; Thermo Fisher Scientific) at 63°C for 30 minutes and examined by naked eyes.

    Article Title: Detecting wMel Wolbachia in field-collected Aedes aegypti mosquitoes using loop-mediated isothermal amplification (LAMP)
    Article Snippet: Individual reaction consisted of 2X WarmStart® Colorimetric LAMP Master Mix (New England BioLabs, Ipswich, USA; Cat# M1800S), primers according to the manufacturer recommendation (Table ), and 1 μl of target DNA in a total reaction volume of 17 μl. .. Reactions for individual samples were performed in 96-well PCR plates (LabAdvantage, Tingalpa, Australia; 96-well PCR plates, full skirt, clear).

    Article Title: Comparative analysis of different molecular and serological methods for detection of Xylella fastidiosa in blueberry
    Article Snippet: LAMP PCR amplification products using LavaLAMP™ master mix (Lucigen) were evaluated by electrophoresis on 1.5% Tris-borate-EDTA (TBE) agarose gel stained with Gelgreen and visualized under ultraviolet (UV) light by Biorad molecular imager Gel Doc™ XR+ with image lab™ software (Biorad, Hercules, CA). .. Finally, the amplified end product from WarmStart® Colorimetric LAMP 2X Master Mix (NEB) was assessed visually with the naked eye.

    Article Title: Rapid and Sensitive Detection of Azole-Resistant Aspergillus fumigatus by Tandem Repeat Loop-Mediated Isothermal Amplification
    Article Snippet: The WarmStart Colorimetric LAMP 2X master mix (New England BioLabs) contains a phenol red pH indicator that provides visual detection of the accumulation of protons during the LAMP reaction, providing a change in solution color from pink (negative) to pale orange (positive). .. LAMP reactions were performed in 0.2-mL PCR tubes in a 25-μL reaction volume, which contains 12.5 μL of WarmStart Colorimetric LAMP 2X master mix, 2.5 μL of TR34-LAMP primer mix (10×), 2.5 μL of gDNA extracted from clinical samples, and 7.5 μL of nuclease-free water.

    Article Title: Scaled deployment ofWolbachia to protect the community from dengue and other Aedes transmitted arboviruses
    Article Snippet: Individual reactions consisted of 2X WarmStart® Colorimetric LAMP Master Mix (New England BioLabs, Cat# M1800S), primers according to the manufacturer recommendation , and 1 μL of target DNA in a total reaction volume of 17 μL. .. Reactions for individual samples were performed in 96-well PCR plates (LabAdvantage 96-well PCR plates, full skirt, clear).

    Article Title: Establishment ofwMelWolbachia inAedes aegypti mosquitoes and reduction of local dengue transmission in Cairns and surrounding locations in northern Queensland, Australia
    Article Snippet: LAMP reactions were performed in a Bio-Rad C1000 96-well PCR thermocycler with a 30min incubation at 65°C as previously described ( ). .. Individual reactions consisted of 2X WarmStart® Colorimetric LAMP Master Mix (New England BioLabs, Cat# M1800S), primers and 1 μL of target DNA in a total reaction volume of 17 μL.

    Incubation:

    Article Title: In Silico Identification of Novel Biomarkers and Development of New Rapid Diagnostic Tests for the Filarial Parasites Mansonella perstans and Mansonella ozzardi
    Article Snippet: LAMP assays LAMP reactions contained 1.6 μM each of primers FIP and BIP, 0.2 μM each of F3 and B3, 0.4 μM each of LF and LB, 12.5 μl of WarmStart Colorimetric LAMP 2X Master Mix (New England Biolabs Inc., USA) with 2 ul of template DNA, or H2 O for non-template controls (NTCs) in a total volume of 25 μl. .. Reactions were incubated at the optimal temperature of 63 °C for 20–30 min (M . ozzardi ) or 60 minutes (M . perstans ) in a T100 Thermal Cycler (Bio-Rad Laboratories, USA).

    Article Title: Detecting wMel Wolbachia in field-collected Aedes aegypti mosquitoes using loop-mediated isothermal amplification (LAMP)
    Article Snippet: Individual reaction consisted of 2X WarmStart® Colorimetric LAMP Master Mix (New England BioLabs, Ipswich, USA; Cat# M1800S), primers according to the manufacturer recommendation (Table ), and 1 μl of target DNA in a total reaction volume of 17 μl. .. Plates were incubated in a thermocycler (BioRad C1000) at 65 °C for 30 min then held at 12 °C until scoring.

    Article Title: Rapid and Sensitive Detection of Azole-Resistant Aspergillus fumigatus by Tandem Repeat Loop-Mediated Isothermal Amplification
    Article Snippet: The WarmStart Colorimetric LAMP 2X master mix (New England BioLabs) contains a phenol red pH indicator that provides visual detection of the accumulation of protons during the LAMP reaction, providing a change in solution color from pink (negative) to pale orange (positive). .. All reaction mixes were incubated in an end-point PCR machine (Veriti Dx 96-well Fast Thermal Cycler; Thermo Fisher Scientific) at 63°C for 30 minutes and examined by naked eyes.

    Article Title: Scaled deployment ofWolbachia to protect the community from dengue and other Aedes transmitted arboviruses
    Article Snippet: Individual reactions consisted of 2X WarmStart® Colorimetric LAMP Master Mix (New England BioLabs, Cat# M1800S), primers according to the manufacturer recommendation , and 1 μL of target DNA in a total reaction volume of 17 μL. .. Plates were incubated in a thermocycler (BioRad C1000) at 65°C for 30 minutes then held at 12°C until scoring.

    Article Title: Establishment ofwMelWolbachia inAedes aegypti mosquitoes and reduction of local dengue transmission in Cairns and surrounding locations in northern Queensland, Australia
    Article Snippet: LAMP reactions were performed in a Bio-Rad C1000 96-well PCR thermocycler with a 30min incubation at 65°C as previously described ( ). .. Individual reactions consisted of 2X WarmStart® Colorimetric LAMP Master Mix (New England BioLabs, Cat# M1800S), primers and 1 μL of target DNA in a total reaction volume of 17 μL.

    Lamp Assay:

    Article Title: Comparative analysis of different molecular and serological methods for detection of Xylella fastidiosa in blueberry
    Article Snippet: Paragraph title: LAMP assay ... For LavaLAMP™ master mix (Lucigen), the primer mixture was comprised of 2 μM of each F3/B3, 8 μM of each LF/LB and 16 μM of each FIP/BIP primers, and for WarmStart® Colorimetric 2X Master Mix (NEB), 2 μM of each F3/B3, 4 μM of each LF/LB and 16 μM of each FIP/BIP primers were used ( ).

    Article Title: Comparative analysis of different molecular and serological methods for detection of Xylella fastidiosa in blueberry
    Article Snippet: .. LAMP assay To detect the pathogen using LAMP amplification, LavaLAMP™ DNA Master Mix (Lucigen, WI, USA) and WarmStart® Colorimetric LAMP 2X Master Mix (New England Bio Labs Ltd., UK) which utilizes pH sensitive phenol red as an end-point indicator, was used according to manufacturer’s instruction. .. Each reaction mixture used in this study contained 12.5 μl 2X master mix, 2.5 μl of primer mix, 50 ng of X . fastidiosa experimental DNA samples and 1 μl samples from serially diluted pure culture (previously described in methods) for sensitivity analysis of the assay for X . fastidiosa detection with the rest were filled with DNase/RNase free PCR certified water (TEKNOVA, Hollister, USA) to a final volume of 25 μl.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Fully 3D Printed Integrated Reactor Array for Point-of-Care Molecular Diagnostics
    Article Snippet: .. Bovine serum albumin (BSA), Poly (ethylene glycol) 8000 (PEG), poly(vinyl alcohol) (PVA) are from Sigma-Aldrich, RT-PCR grade water from Ambion, Inc., intercalating Eva Green fluorescent dye from Biotium, Isothermal Master Mix used in LAMP reaction buffer from ISO-001nd, OptiGene, Horsham, UK, WarmStart® colorimetric LAMP 2X master mix from New England Biolabs Inc. N. meningitidis (ATCC 13098) from American Type Culture Collection (ATCC, Rockville, MD). .. N. meningitidis was grown in Tryptic Soy Broth (BD, Sparks, MD) and counted on Trypticase™ Soy Agar with 5% Sheep Blood (BD, Sparks, MD).

    Staining:

    Article Title: Comparative analysis of different molecular and serological methods for detection of Xylella fastidiosa in blueberry
    Article Snippet: For LavaLAMP™ master mix (Lucigen), the primer mixture was comprised of 2 μM of each F3/B3, 8 μM of each LF/LB and 16 μM of each FIP/BIP primers, and for WarmStart® Colorimetric 2X Master Mix (NEB), 2 μM of each F3/B3, 4 μM of each LF/LB and 16 μM of each FIP/BIP primers were used ( ). .. LAMP PCR amplification products using LavaLAMP™ master mix (Lucigen) were evaluated by electrophoresis on 1.5% Tris-borate-EDTA (TBE) agarose gel stained with Gelgreen and visualized under ultraviolet (UV) light by Biorad molecular imager Gel Doc™ XR+ with image lab™ software (Biorad, Hercules, CA).

    Real-time Polymerase Chain Reaction:

    Article Title: In Silico Identification of Novel Biomarkers and Development of New Rapid Diagnostic Tests for the Filarial Parasites Mansonella perstans and Mansonella ozzardi
    Article Snippet: LAMP assays LAMP reactions contained 1.6 μM each of primers FIP and BIP, 0.2 μM each of F3 and B3, 0.4 μM each of LF and LB, 12.5 μl of WarmStart Colorimetric LAMP 2X Master Mix (New England Biolabs Inc., USA) with 2 ul of template DNA, or H2 O for non-template controls (NTCs) in a total volume of 25 μl. .. When a qPCR machine (CFX-96 Touch Thermal Cycler, Bio-Rad Laboratories, USA) was used to enable reaction dynamics to be monitored in real time, colorimetric reactions also contained 1X LAMP Fluorescent Dye (New England Biolabs Inc., USA) and were incubated at 63 °C for ~30 min (M . ozzardi : 36 cycles) or ~1 hr (M . perstans : 72 cycles) with a plate read step every 42 seconds.

    Article Title: Scaled deployment ofWolbachia to protect the community from dengue and other Aedes transmitted arboviruses
    Article Snippet: Adult Ae. aegypti samples collected from BGS traps in the field were screened for Wolbachia using Taqman qPCR on a Roche LightCycler 480 using a qualitative assay for presence or absence of Wolbachia as previously described but with the replacement of the Cy5-BHQ3 fluorophore-quencher pair in the w Mel probe with the fluorophore-quencher LC640-IowaBlack (Integrated DNA technologies) to remove some of the Cy5 probe instability observed under varying light and ozone levels . .. Individual reactions consisted of 2X WarmStart® Colorimetric LAMP Master Mix (New England BioLabs, Cat# M1800S), primers according to the manufacturer recommendation , and 1 μL of target DNA in a total reaction volume of 17 μL.

    Article Title: Establishment ofwMelWolbachia inAedes aegypti mosquitoes and reduction of local dengue transmission in Cairns and surrounding locations in northern Queensland, Australia
    Article Snippet: The qPCR cycling program consisted of a denaturation at 95°C for 5 min followed by 45 cycles of PCR (denaturation at 95 °C for 10 sec, annealing at 60 °C for 15 sec, and extension at 72 °C for 1 sec with single acquisition) followed by a cooling down step at 40°C for 10 sec. From September 2018Wolbachia diagnostics were performed by LAMP. .. Individual reactions consisted of 2X WarmStart® Colorimetric LAMP Master Mix (New England BioLabs, Cat# M1800S), primers and 1 μL of target DNA in a total reaction volume of 17 μL.

    Binding Assay:

    Article Title: Simpler, Faster, and Sensitive Zika Virus Assay Using Smartphone Detection of Loop-mediated Isothermal Amplification on Paper Microfluidic Chips
    Article Snippet: Furthermore, the demonstrated colorimetric detection was based on a streptavidin-biotin binding mechanism on gold nanoparticles, potentially causing non-specific cross-binding with other molecules and/or targets. .. For this purpose, pH indicator-based colorimetric assay kits could be used (e.g., WarmStart colorimetric LAMP master mix by New England Biolabs), which includes a pH indicator (phenol red) in the master mix reagent, requiring no additional step, no excitation light source, and no closed container (identifiable under ambient light) .

    Software:

    Article Title: Comparative analysis of different molecular and serological methods for detection of Xylella fastidiosa in blueberry
    Article Snippet: For LavaLAMP™ master mix (Lucigen), the primer mixture was comprised of 2 μM of each F3/B3, 8 μM of each LF/LB and 16 μM of each FIP/BIP primers, and for WarmStart® Colorimetric 2X Master Mix (NEB), 2 μM of each F3/B3, 4 μM of each LF/LB and 16 μM of each FIP/BIP primers were used ( ). .. LAMP PCR amplification products using LavaLAMP™ master mix (Lucigen) were evaluated by electrophoresis on 1.5% Tris-borate-EDTA (TBE) agarose gel stained with Gelgreen and visualized under ultraviolet (UV) light by Biorad molecular imager Gel Doc™ XR+ with image lab™ software (Biorad, Hercules, CA).

    Article Title: Detecting wMel Wolbachia in field-collected Aedes aegypti mosquitoes using loop-mediated isothermal amplification (LAMP)
    Article Snippet: w Mel LAMP reactions LAMP primers (Integrated DNA Technologies, Singapore, Singapore) were designed to detect the wsp gene from w Mel and w MelPop-CLA strains using the software LAMP Designer 1.02 (PREMIER Biosoft International, Horsham, UK). .. Individual reaction consisted of 2X WarmStart® Colorimetric LAMP Master Mix (New England BioLabs, Ipswich, USA; Cat# M1800S), primers according to the manufacturer recommendation (Table ), and 1 μl of target DNA in a total reaction volume of 17 μl.

    Article Title: Scaled deployment ofWolbachia to protect the community from dengue and other Aedes transmitted arboviruses
    Article Snippet: LAMP primers (Integrated DNA Technologies, Singapore) were designed to detect the wsp gene from w Mel and w MelPop-CLA strains using the software LAMP Designer 1.02 (PREMIER Biosoft International). .. Individual reactions consisted of 2X WarmStart® Colorimetric LAMP Master Mix (New England BioLabs, Cat# M1800S), primers according to the manufacturer recommendation , and 1 μL of target DNA in a total reaction volume of 17 μL.

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    • warmstart colorimetric lamp 2x master mix  (New England Biolabs)
    95
    New England Biolabs warmstart colorimetric lamp 2x master mix
    Colorimetric and fluorescence based detection for NAATs in 3D printed reactor array. A) Representative photographs of colorimetric <t>LAMP</t> assay for detection of N. meningitidis with 0, 50, 500 and 5000 CFU/reaction on the same chip, alongside LAMP fluorescence based image at given time interval. B) LAMP amplification curves for P. falciparum with 0, 0.1 1, 10, 100, 1000 pg per reaction. C) Calibration curve for P. falciparum as function of log target concentration, n=3. D) LAMP amplification curves for N. meningitidis with 0, 50, 500, 5000 CFU per reaction. E) Calibration curve for N. meningitidis as function of log target concentration, n=3. <t>WarmStart</t> ® LAMP master mix was used.
    Warmstart Colorimetric Lamp 2x Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/warmstart colorimetric lamp 2x master mix/product/New England Biolabs
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    warmstart colorimetric lamp 2x master mix - by Bioz Stars, 2020-02
    95/100 stars
    		  Buy from Supplier

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    Colorimetric and fluorescence based detection for NAATs in 3D printed reactor array. A) Representative photographs of colorimetric LAMP assay for detection of N. meningitidis with 0, 50, 500 and 5000 CFU/reaction on the same chip, alongside LAMP fluorescence based image at given time interval. B) LAMP amplification curves for P. falciparum with 0, 0.1 1, 10, 100, 1000 pg per reaction. C) Calibration curve for P. falciparum as function of log target concentration, n=3. D) LAMP amplification curves for N. meningitidis with 0, 50, 500, 5000 CFU per reaction. E) Calibration curve for N. meningitidis as function of log target concentration, n=3. WarmStart ® LAMP master mix was used.

    Journal: Biosensors & bioelectronics

    Article Title: Fully 3D Printed Integrated Reactor Array for Point-of-Care Molecular Diagnostics

    doi: 10.1016/j.bios.2018.03.009

    Figure Lengend Snippet: Colorimetric and fluorescence based detection for NAATs in 3D printed reactor array. A) Representative photographs of colorimetric LAMP assay for detection of N. meningitidis with 0, 50, 500 and 5000 CFU/reaction on the same chip, alongside LAMP fluorescence based image at given time interval. B) LAMP amplification curves for P. falciparum with 0, 0.1 1, 10, 100, 1000 pg per reaction. C) Calibration curve for P. falciparum as function of log target concentration, n=3. D) LAMP amplification curves for N. meningitidis with 0, 50, 500, 5000 CFU per reaction. E) Calibration curve for N. meningitidis as function of log target concentration, n=3. WarmStart ® LAMP master mix was used.

    Article Snippet: Bovine serum albumin (BSA), Poly (ethylene glycol) 8000 (PEG), poly(vinyl alcohol) (PVA) are from Sigma-Aldrich, RT-PCR grade water from Ambion, Inc., intercalating Eva Green fluorescent dye from Biotium, Isothermal Master Mix used in LAMP reaction buffer from ISO-001nd, OptiGene, Horsham, UK, WarmStart® colorimetric LAMP 2X master mix from New England Biolabs Inc. N. meningitidis (ATCC 13098) from American Type Culture Collection (ATCC, Rockville, MD).

    Techniques: Fluorescence, Lamp Assay, Chromatin Immunoprecipitation, Amplification, Concentration Assay