warmstart colorimetric lamp 2x master mix  (New England Biolabs)


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    • 95
    Name:
    WarmStart Colorimetric LAMP 2X Master Mix DNA and RNA
    Description:
    WarmStart Colorimetric LAMP 2X Master Mix DNA and RNA 500 rxns
    Catalog Number:
    m1800l
    Price:
    832
    Size:
    500 rxns
    Category:
    Thermostable DNA Polymerases
    		Buy from Supplier

    Structured Review

    New England Biolabs warmstart colorimetric lamp 2x master mix
    WarmStart Colorimetric LAMP 2X Master Mix DNA and RNA
    WarmStart Colorimetric LAMP 2X Master Mix DNA and RNA 500 rxns
    https://www.bioz.com/result/warmstart colorimetric lamp 2x master mix/product/New England Biolabs
    Average 95 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    warmstart colorimetric lamp 2x master mix - by Bioz Stars, 2020-05
    95/100 stars

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    2x colorimetric lamp master mix

    Images

    1) Product Images from "Fully 3D Printed Integrated Reactor Array for Point-of-Care Molecular Diagnostics"

    Article Title: Fully 3D Printed Integrated Reactor Array for Point-of-Care Molecular Diagnostics

    Journal: Biosensors & bioelectronics

    doi: 10.1016/j.bios.2018.03.009

    Colorimetric and fluorescence based detection for NAATs in 3D printed reactor array. A) Representative photographs of colorimetric LAMP assay for detection of N. meningitidis with 0, 50, 500 and 5000 CFU/reaction on the same chip, alongside LAMP fluorescence based image at given time interval. B) LAMP amplification curves for P. falciparum with 0, 0.1 1, 10, 100, 1000 pg per reaction. C) Calibration curve for P. falciparum as function of log target concentration, n=3. D) LAMP amplification curves for N. meningitidis with 0, 50, 500, 5000 CFU per reaction. E) Calibration curve for N. meningitidis as function of log target concentration, n=3. WarmStart ® LAMP master mix was used.
    Figure Legend Snippet: Colorimetric and fluorescence based detection for NAATs in 3D printed reactor array. A) Representative photographs of colorimetric LAMP assay for detection of N. meningitidis with 0, 50, 500 and 5000 CFU/reaction on the same chip, alongside LAMP fluorescence based image at given time interval. B) LAMP amplification curves for P. falciparum with 0, 0.1 1, 10, 100, 1000 pg per reaction. C) Calibration curve for P. falciparum as function of log target concentration, n=3. D) LAMP amplification curves for N. meningitidis with 0, 50, 500, 5000 CFU per reaction. E) Calibration curve for N. meningitidis as function of log target concentration, n=3. WarmStart ® LAMP master mix was used.

    Techniques Used: Fluorescence, Lamp Assay, Chromatin Immunoprecipitation, Amplification, Concentration Assay

    Related Articles

    RT Lamp Assay:

    Article Title: A Mismatch-Tolerant Reverse Transcription Loop-Mediated Isothermal Amplification Method and Its Application on Simultaneous Detection of All Four Serotype of Dengue Viruses
    Article Snippet: .. Another visual RT-LAMP assay was performed with the WarmStart Colorimetric LAMP 2X Master Mix (New England Biolabs, Beverly, MA, United States), which uses cresol red as a visual indicator. ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Fully 3D Printed Integrated Reactor Array for Point-of-Care Molecular Diagnostics
    Article Snippet: .. Bovine serum albumin (BSA), Poly (ethylene glycol) 8000 (PEG), poly(vinyl alcohol) (PVA) are from Sigma-Aldrich, RT-PCR grade water from Ambion, Inc., intercalating Eva Green fluorescent dye from Biotium, Isothermal Master Mix used in LAMP reaction buffer from ISO-001nd, OptiGene, Horsham, UK, WarmStart® colorimetric LAMP 2X master mix from New England Biolabs Inc. N. meningitidis (ATCC 13098) from American Type Culture Collection (ATCC, Rockville, MD). .. N. meningitidis was grown in Tryptic Soy Broth (BD, Sparks, MD) and counted on Trypticase™ Soy Agar with 5% Sheep Blood (BD, Sparks, MD).

    Amplification:

    Article Title: Comparative analysis of different molecular and serological methods for detection of Xylella fastidiosa in blueberry
    Article Snippet: .. LAMP assay To detect the pathogen using LAMP amplification, LavaLAMP™ DNA Master Mix (Lucigen, WI, USA) and WarmStart® Colorimetric LAMP 2X Master Mix (New England Bio Labs Ltd., UK) which utilizes pH sensitive phenol red as an end-point indicator, was used according to manufacturer’s instruction. .. Each reaction mixture used in this study contained 12.5 μl 2X master mix, 2.5 μl of primer mix, 50 ng of X . fastidiosa experimental DNA samples and 1 μl samples from serially diluted pure culture (previously described in methods) for sensitivity analysis of the assay for X . fastidiosa detection with the rest were filled with DNase/RNase free PCR certified water (TEKNOVA, Hollister, USA) to a final volume of 25 μl.

    Lamp Assay:

    Article Title: Comparative analysis of different molecular and serological methods for detection of Xylella fastidiosa in blueberry
    Article Snippet: .. LAMP assay To detect the pathogen using LAMP amplification, LavaLAMP™ DNA Master Mix (Lucigen, WI, USA) and WarmStart® Colorimetric LAMP 2X Master Mix (New England Bio Labs Ltd., UK) which utilizes pH sensitive phenol red as an end-point indicator, was used according to manufacturer’s instruction. .. Each reaction mixture used in this study contained 12.5 μl 2X master mix, 2.5 μl of primer mix, 50 ng of X . fastidiosa experimental DNA samples and 1 μl samples from serially diluted pure culture (previously described in methods) for sensitivity analysis of the assay for X . fastidiosa detection with the rest were filled with DNase/RNase free PCR certified water (TEKNOVA, Hollister, USA) to a final volume of 25 μl.

    Colorimetric Assay:

    Article Title: Simpler, Faster, and Sensitive Zika Virus Assay Using Smartphone Detection of Loop-mediated Isothermal Amplification on Paper Microfluidic Chips
    Article Snippet: .. For this purpose, pH indicator-based colorimetric assay kits could be used (e.g., WarmStart colorimetric LAMP master mix by New England Biolabs), which includes a pH indicator (phenol red) in the master mix reagent, requiring no additional step, no excitation light source, and no closed container (identifiable under ambient light) . ..

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    • warmstart colorimetric lamp 2x master mix  (New England Biolabs)
    95
    New England Biolabs warmstart colorimetric lamp 2x master mix
    Colorimetric and fluorescence based detection for NAATs in 3D printed reactor array. A) Representative photographs of colorimetric <t>LAMP</t> assay for detection of N. meningitidis with 0, 50, 500 and 5000 CFU/reaction on the same chip, alongside LAMP fluorescence based image at given time interval. B) LAMP amplification curves for P. falciparum with 0, 0.1 1, 10, 100, 1000 pg per reaction. C) Calibration curve for P. falciparum as function of log target concentration, n=3. D) LAMP amplification curves for N. meningitidis with 0, 50, 500, 5000 CFU per reaction. E) Calibration curve for N. meningitidis as function of log target concentration, n=3. <t>WarmStart</t> ® LAMP master mix was used.
    Warmstart Colorimetric Lamp 2x Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/warmstart colorimetric lamp 2x master mix/product/New England Biolabs
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    warmstart colorimetric lamp 2x master mix - by Bioz Stars, 2020-05
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    Colorimetric and fluorescence based detection for NAATs in 3D printed reactor array. A) Representative photographs of colorimetric LAMP assay for detection of N. meningitidis with 0, 50, 500 and 5000 CFU/reaction on the same chip, alongside LAMP fluorescence based image at given time interval. B) LAMP amplification curves for P. falciparum with 0, 0.1 1, 10, 100, 1000 pg per reaction. C) Calibration curve for P. falciparum as function of log target concentration, n=3. D) LAMP amplification curves for N. meningitidis with 0, 50, 500, 5000 CFU per reaction. E) Calibration curve for N. meningitidis as function of log target concentration, n=3. WarmStart ® LAMP master mix was used.

    Journal: Biosensors & bioelectronics

    Article Title: Fully 3D Printed Integrated Reactor Array for Point-of-Care Molecular Diagnostics

    doi: 10.1016/j.bios.2018.03.009

    Figure Lengend Snippet: Colorimetric and fluorescence based detection for NAATs in 3D printed reactor array. A) Representative photographs of colorimetric LAMP assay for detection of N. meningitidis with 0, 50, 500 and 5000 CFU/reaction on the same chip, alongside LAMP fluorescence based image at given time interval. B) LAMP amplification curves for P. falciparum with 0, 0.1 1, 10, 100, 1000 pg per reaction. C) Calibration curve for P. falciparum as function of log target concentration, n=3. D) LAMP amplification curves for N. meningitidis with 0, 50, 500, 5000 CFU per reaction. E) Calibration curve for N. meningitidis as function of log target concentration, n=3. WarmStart ® LAMP master mix was used.

    Article Snippet: Bovine serum albumin (BSA), Poly (ethylene glycol) 8000 (PEG), poly(vinyl alcohol) (PVA) are from Sigma-Aldrich, RT-PCR grade water from Ambion, Inc., intercalating Eva Green fluorescent dye from Biotium, Isothermal Master Mix used in LAMP reaction buffer from ISO-001nd, OptiGene, Horsham, UK, WarmStart® colorimetric LAMP 2X master mix from New England Biolabs Inc. N. meningitidis (ATCC 13098) from American Type Culture Collection (ATCC, Rockville, MD).

    Techniques: Fluorescence, Lamp Assay, Chromatin Immunoprecipitation, Amplification, Concentration Assay