tte uvrd helicase  (New England Biolabs)


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    Name:
    Tte UvrD Helicase
    Description:
    Tte UvrD Helicase is a thermostable helicase UvrD from Thermoanaerobacter tengcongensis used for suppressing non template nonspecific amplification in isothermal reactions mainly LAMP It is compatible with all LAMP and RT LAMP reactions and can eliminate false positives and non template control amplification without affecting positive reactions Target customers are diagnostic LAMP users
    Catalog Number:
    M1202S
    Price:
    72
    Category:
    Proteases
    Size:
    50 rxns
    Buy from Supplier


    Structured Review

    New England Biolabs tte uvrd helicase
    Tte UvrD Helicase
    Tte UvrD Helicase is a thermostable helicase UvrD from Thermoanaerobacter tengcongensis used for suppressing non template nonspecific amplification in isothermal reactions mainly LAMP It is compatible with all LAMP and RT LAMP reactions and can eliminate false positives and non template control amplification without affecting positive reactions Target customers are diagnostic LAMP users
    https://www.bioz.com/result/tte uvrd helicase/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tte uvrd helicase - by Bioz Stars, 2021-06
    95/100 stars

    Images

    1) Product Images from "A Single-Tube HNB-Based Loop-Mediated Isothermal Amplification for the Robust Detection of the Ostreid herpesvirus 1"

    Article Title: A Single-Tube HNB-Based Loop-Mediated Isothermal Amplification for the Robust Detection of the Ostreid herpesvirus 1

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms21186605

    LAMP primer evaluation for amplification of the OsHV-1 plasmid (T = 65 °C, t = 10 min) and sensitivity with the addition of Tte UvrD helicase enzyme: ( A ) electropherogram comparison profiles of positive (OsHV-1 plasmid) and negative templates (NTC, UN_1 and AbHV) analysed using Tape Station Analysis Software A.02.02 (Agilent Technologies) for assessment of progressive increase of amplified LAMP product. Positive templates show a size shift of 350–5000 bp. ( B ) Colorimetric assessment of amplified LAMP products for positive (OsHV-1) (pink) and NTC (violet) templates using HNB.
    Figure Legend Snippet: LAMP primer evaluation for amplification of the OsHV-1 plasmid (T = 65 °C, t = 10 min) and sensitivity with the addition of Tte UvrD helicase enzyme: ( A ) electropherogram comparison profiles of positive (OsHV-1 plasmid) and negative templates (NTC, UN_1 and AbHV) analysed using Tape Station Analysis Software A.02.02 (Agilent Technologies) for assessment of progressive increase of amplified LAMP product. Positive templates show a size shift of 350–5000 bp. ( B ) Colorimetric assessment of amplified LAMP products for positive (OsHV-1) (pink) and NTC (violet) templates using HNB.

    Techniques Used: Amplification, Plasmid Preparation, Software

    2) Product Images from "A Single-Tube HNB-Based Loop-Mediated Isothermal Amplification for the Robust Detection of the Ostreid herpesvirus 1"

    Article Title: A Single-Tube HNB-Based Loop-Mediated Isothermal Amplification for the Robust Detection of the Ostreid herpesvirus 1

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms21186605

    LAMP primer evaluation for amplification of the OsHV-1 plasmid (T = 65 °C, t = 10 min) and sensitivity with the addition of Tte UvrD helicase enzyme: ( A ) electropherogram comparison profiles of positive (OsHV-1 plasmid) and negative templates (NTC, UN_1 and AbHV) analysed using Tape Station Analysis Software A.02.02 (Agilent Technologies) for assessment of progressive increase of amplified LAMP product. Positive templates show a size shift of 350–5000 bp. ( B ) Colorimetric assessment of amplified LAMP products for positive (OsHV-1) (pink) and NTC (violet) templates using HNB.
    Figure Legend Snippet: LAMP primer evaluation for amplification of the OsHV-1 plasmid (T = 65 °C, t = 10 min) and sensitivity with the addition of Tte UvrD helicase enzyme: ( A ) electropherogram comparison profiles of positive (OsHV-1 plasmid) and negative templates (NTC, UN_1 and AbHV) analysed using Tape Station Analysis Software A.02.02 (Agilent Technologies) for assessment of progressive increase of amplified LAMP product. Positive templates show a size shift of 350–5000 bp. ( B ) Colorimetric assessment of amplified LAMP products for positive (OsHV-1) (pink) and NTC (violet) templates using HNB.

    Techniques Used: Amplification, Plasmid Preparation, Software

    3) Product Images from "A Single-Tube HNB-Based Loop-Mediated Isothermal Amplification for the Robust Detection of the Ostreid herpesvirus 1"

    Article Title: A Single-Tube HNB-Based Loop-Mediated Isothermal Amplification for the Robust Detection of the Ostreid herpesvirus 1

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms21186605

    LAMP primer evaluation for amplification of the OsHV-1 plasmid (T = 65 °C, t = 10 min) and sensitivity with the addition of Tte UvrD helicase enzyme: ( A ) electropherogram comparison profiles of positive (OsHV-1 plasmid) and negative templates (NTC, UN_1 and AbHV) analysed using Tape Station Analysis Software A.02.02 (Agilent Technologies) for assessment of progressive increase of amplified LAMP product. Positive templates show a size shift of 350–5000 bp. ( B ) Colorimetric assessment of amplified LAMP products for positive (OsHV-1) (pink) and NTC (violet) templates using HNB.
    Figure Legend Snippet: LAMP primer evaluation for amplification of the OsHV-1 plasmid (T = 65 °C, t = 10 min) and sensitivity with the addition of Tte UvrD helicase enzyme: ( A ) electropherogram comparison profiles of positive (OsHV-1 plasmid) and negative templates (NTC, UN_1 and AbHV) analysed using Tape Station Analysis Software A.02.02 (Agilent Technologies) for assessment of progressive increase of amplified LAMP product. Positive templates show a size shift of 350–5000 bp. ( B ) Colorimetric assessment of amplified LAMP products for positive (OsHV-1) (pink) and NTC (violet) templates using HNB.

    Techniques Used: Amplification, Plasmid Preparation, Software

    4) Product Images from "A Single-Tube HNB-Based Loop-Mediated Isothermal Amplification for the Robust Detection of the Ostreid herpesvirus 1"

    Article Title: A Single-Tube HNB-Based Loop-Mediated Isothermal Amplification for the Robust Detection of the Ostreid herpesvirus 1

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms21186605

    LAMP primer evaluation for amplification of the OsHV-1 plasmid (T = 65 °C, t = 10 min) and sensitivity with the addition of Tte UvrD helicase enzyme: ( A ) electropherogram comparison profiles of positive (OsHV-1 plasmid) and negative templates (NTC, UN_1 and AbHV) analysed using Tape Station Analysis Software A.02.02 (Agilent Technologies) for assessment of progressive increase of amplified LAMP product. Positive templates show a size shift of 350–5000 bp. ( B ) Colorimetric assessment of amplified LAMP products for positive (OsHV-1) (pink) and NTC (violet) templates using HNB.
    Figure Legend Snippet: LAMP primer evaluation for amplification of the OsHV-1 plasmid (T = 65 °C, t = 10 min) and sensitivity with the addition of Tte UvrD helicase enzyme: ( A ) electropherogram comparison profiles of positive (OsHV-1 plasmid) and negative templates (NTC, UN_1 and AbHV) analysed using Tape Station Analysis Software A.02.02 (Agilent Technologies) for assessment of progressive increase of amplified LAMP product. Positive templates show a size shift of 350–5000 bp. ( B ) Colorimetric assessment of amplified LAMP products for positive (OsHV-1) (pink) and NTC (violet) templates using HNB.

    Techniques Used: Amplification, Plasmid Preparation, Software

    Related Articles

    Lamp Assay:

    Article Title: A Single-Tube HNB-Based Loop-Mediated Isothermal Amplification for the Robust Detection of the Ostreid herpesvirus 1
    Article Snippet: .. Tte UvrD Helicase Lamp Assay The total reaction volume of 25 μL contained 1.6 μM/µL of each FIP/BIP primer, 0.2 μM of each F3/B3 (Thermo Fisher Scientific, Waltham, MA, USA), 1.4 mM of dNTPs (Thermo Fisher Scientific, Waltham, MA, USA), 1 M of betaine (Sigma Aldrich, St. Louis, MO, USA), 8 mM of MgSO4 (New England BioLabs, Hitchin, UK), 1× ThermoPol reaction buffer (New England BioLabs, Hitchin, UK) (20 mM Tris–HCl, 10 mM KCl, 10 mM (NH4)2 SO4 and 0.1% Triton X-100), 320 U/mL of Bst DNA polymerase large fragment (New England BioLabs, Hitchin, UK), Tte UvrD helicase 20 ng/mL (New England BioLabs, Hitchin, UK) HNB dye 120 µM and 2 μL of OsHV-1 DNA plasmid template (1 ng/μL). .. The reaction mixture was incubated 65 °C for 10 min in a thermo-cycler (Bio-Rad, Watford, UK) and then heated to 80 °C for 5 min to terminate the reaction.

    Article Title: Advances in point-of-care nucleic acid extraction technologies for rapid diagnosis of human and plant diseases
    Article Snippet: .. Recently, several modified versions of the LAMP assay such as Tte UvrD Helicase-LAMP (New England Biolabs, USA) and UDG-LAMP ( ) have been demonstrated to further improve the specificity and other drawbacks of the assay. ..

    Plasmid Preparation:

    Article Title: A Single-Tube HNB-Based Loop-Mediated Isothermal Amplification for the Robust Detection of the Ostreid herpesvirus 1
    Article Snippet: .. Tte UvrD Helicase Lamp Assay The total reaction volume of 25 μL contained 1.6 μM/µL of each FIP/BIP primer, 0.2 μM of each F3/B3 (Thermo Fisher Scientific, Waltham, MA, USA), 1.4 mM of dNTPs (Thermo Fisher Scientific, Waltham, MA, USA), 1 M of betaine (Sigma Aldrich, St. Louis, MO, USA), 8 mM of MgSO4 (New England BioLabs, Hitchin, UK), 1× ThermoPol reaction buffer (New England BioLabs, Hitchin, UK) (20 mM Tris–HCl, 10 mM KCl, 10 mM (NH4)2 SO4 and 0.1% Triton X-100), 320 U/mL of Bst DNA polymerase large fragment (New England BioLabs, Hitchin, UK), Tte UvrD helicase 20 ng/mL (New England BioLabs, Hitchin, UK) HNB dye 120 µM and 2 μL of OsHV-1 DNA plasmid template (1 ng/μL). .. The reaction mixture was incubated 65 °C for 10 min in a thermo-cycler (Bio-Rad, Watford, UK) and then heated to 80 °C for 5 min to terminate the reaction.

    Amplification:

    Article Title: A Single-Tube HNB-Based Loop-Mediated Isothermal Amplification for the Robust Detection of the Ostreid herpesvirus 1
    Article Snippet: Interestingly, fluorescence intensity measured using Agilent 2200 Tape Station indicated an increase in the suppression of NTC and two other negative samples and a lower intensity for positive samples than Bst DNA polymerase-based LAMP assay ( A). .. As indicated previously, in the presence of Tte UvrD Helicase, the positive reaction maintains its rapid amplification time with a slight reduction in total fluorescence while the negative reaction is completely suppressed [ ]. ..

    Article Title: A Single-Tube HNB-Based Loop-Mediated Isothermal Amplification for the Robust Detection of the Ostreid herpesvirus 1
    Article Snippet: In contrast, the optimal amplification using only Bst DNA polymerase was reached after 60 min, although a shorter LAMP reaction time of 45 min has been reported previously [ ]. .. This fast amplification using Tte UvrD helicase is in agreement with the producer (New England Biolabs) of the technical protocols ensuring a 10-min reaction [ ]. .. However, in this study, LAMP Thermopol Master Mix was used instead of Warm Master Mix (New England Biolabs) as recommended, indicating efficient activity of the enzyme with Thermopol buffer and other ingredients composition.

    Fluorescence:

    Article Title: A Single-Tube HNB-Based Loop-Mediated Isothermal Amplification for the Robust Detection of the Ostreid herpesvirus 1
    Article Snippet: Interestingly, fluorescence intensity measured using Agilent 2200 Tape Station indicated an increase in the suppression of NTC and two other negative samples and a lower intensity for positive samples than Bst DNA polymerase-based LAMP assay ( A). .. As indicated previously, in the presence of Tte UvrD Helicase, the positive reaction maintains its rapid amplification time with a slight reduction in total fluorescence while the negative reaction is completely suppressed [ ]. ..

    Helicase-dependent Amplification:

    Article Title: Automated Detection of Toxigenic Clostridium difficile in Clinical Samples: Isothermal tcdB Amplification Coupled to Array-Based Detection
    Article Snippet: Swabs taken from unformed stool samples were vortexed in extraction buffer ([EB] 750 μl of phosphate-buffered saline [PBS]–0.01% Tween 20), filtered using a 3-ml syringe, and heated at 95°C for 5 min in 20 mM Tris-HCl, pH 8.8, 10 mM KCl, 7.7 mM MgSO4 , 40 mM NaCl, 5 mg/ml bovine serum albumin (BSA), and 0.02% Tween 20 (Sigma Aldrich, St. Louis, MO). .. Twenty microliters was added to 20 μl of 2× blocked-primer HDA (bpHDA) mix (20 mM Tris-HCl, pH 8.8, 40 mM NaCl, 0.8 mM each dCTP, dGTP, and dTTP, and 6.8 mM dATP), 10 ng/μl thermostable UvrD helicase (Tte-UvrD; BioHelix), 1.6 U/μl glutathione S -transferase (GST) polymerase (New England BioLabs, Ipswich, MA), 2 μl of RN2 Master Mix (Great Basin Corporation), 4 ng/μl extreme thermostable single-stranded DNA binding protein ([ET SSB] New England BioLabs), EvaGreen (used at 0.4×; 1× is the manufacturer-recommended final dilution; Biotium, Hayward, CA), 400 nM and 800 nM tcdB forward and reverse primer, respectively, and 200 nM and 400 nM nuc primers and incubated for 45 min at 65°C (LightCycler 480; Roche, Basel). ..

    Binding Assay:

    Article Title: Automated Detection of Toxigenic Clostridium difficile in Clinical Samples: Isothermal tcdB Amplification Coupled to Array-Based Detection
    Article Snippet: Swabs taken from unformed stool samples were vortexed in extraction buffer ([EB] 750 μl of phosphate-buffered saline [PBS]–0.01% Tween 20), filtered using a 3-ml syringe, and heated at 95°C for 5 min in 20 mM Tris-HCl, pH 8.8, 10 mM KCl, 7.7 mM MgSO4 , 40 mM NaCl, 5 mg/ml bovine serum albumin (BSA), and 0.02% Tween 20 (Sigma Aldrich, St. Louis, MO). .. Twenty microliters was added to 20 μl of 2× blocked-primer HDA (bpHDA) mix (20 mM Tris-HCl, pH 8.8, 40 mM NaCl, 0.8 mM each dCTP, dGTP, and dTTP, and 6.8 mM dATP), 10 ng/μl thermostable UvrD helicase (Tte-UvrD; BioHelix), 1.6 U/μl glutathione S -transferase (GST) polymerase (New England BioLabs, Ipswich, MA), 2 μl of RN2 Master Mix (Great Basin Corporation), 4 ng/μl extreme thermostable single-stranded DNA binding protein ([ET SSB] New England BioLabs), EvaGreen (used at 0.4×; 1× is the manufacturer-recommended final dilution; Biotium, Hayward, CA), 400 nM and 800 nM tcdB forward and reverse primer, respectively, and 200 nM and 400 nM nuc primers and incubated for 45 min at 65°C (LightCycler 480; Roche, Basel). ..

    Incubation:

    Article Title: Automated Detection of Toxigenic Clostridium difficile in Clinical Samples: Isothermal tcdB Amplification Coupled to Array-Based Detection
    Article Snippet: Swabs taken from unformed stool samples were vortexed in extraction buffer ([EB] 750 μl of phosphate-buffered saline [PBS]–0.01% Tween 20), filtered using a 3-ml syringe, and heated at 95°C for 5 min in 20 mM Tris-HCl, pH 8.8, 10 mM KCl, 7.7 mM MgSO4 , 40 mM NaCl, 5 mg/ml bovine serum albumin (BSA), and 0.02% Tween 20 (Sigma Aldrich, St. Louis, MO). .. Twenty microliters was added to 20 μl of 2× blocked-primer HDA (bpHDA) mix (20 mM Tris-HCl, pH 8.8, 40 mM NaCl, 0.8 mM each dCTP, dGTP, and dTTP, and 6.8 mM dATP), 10 ng/μl thermostable UvrD helicase (Tte-UvrD; BioHelix), 1.6 U/μl glutathione S -transferase (GST) polymerase (New England BioLabs, Ipswich, MA), 2 μl of RN2 Master Mix (Great Basin Corporation), 4 ng/μl extreme thermostable single-stranded DNA binding protein ([ET SSB] New England BioLabs), EvaGreen (used at 0.4×; 1× is the manufacturer-recommended final dilution; Biotium, Hayward, CA), 400 nM and 800 nM tcdB forward and reverse primer, respectively, and 200 nM and 400 nM nuc primers and incubated for 45 min at 65°C (LightCycler 480; Roche, Basel). ..

    Modification:

    Article Title: Advances in point-of-care nucleic acid extraction technologies for rapid diagnosis of human and plant diseases
    Article Snippet: .. Recently, several modified versions of the LAMP assay such as Tte UvrD Helicase-LAMP (New England Biolabs, USA) and UDG-LAMP ( ) have been demonstrated to further improve the specificity and other drawbacks of the assay. ..

    Functional Assay:

    Article Title: A Single-Tube HNB-Based Loop-Mediated Isothermal Amplification for the Robust Detection of the Ostreid herpesvirus 1
    Article Snippet: The reagents were standardized in various concentrations , which were previously reported as optimal reagents concentration for LAMP assays. .. Two different protocols based on ThermoPol Reaction Buffer Pack (New England BioLabs, Hitchin, UK) were assessed using two compatible enzymes: thermophilic Bst DNA polymerase large fragment (100% functional activity) (New England BioLabs, Hitchin, UK) and Tte UvrD helicase (100% functional activity) (New England BioLabs, Hitchin, UK). .. The assays were assessed based on HNB-visualized colour change and/or by using Agilent 2200 Tape Station (Agilent Technologies, Cheshire, UK) High Sensitivity D5000 DNA following manufacturer instructions.

    Activity Assay:

    Article Title: A Single-Tube HNB-Based Loop-Mediated Isothermal Amplification for the Robust Detection of the Ostreid herpesvirus 1
    Article Snippet: The reagents were standardized in various concentrations , which were previously reported as optimal reagents concentration for LAMP assays. .. Two different protocols based on ThermoPol Reaction Buffer Pack (New England BioLabs, Hitchin, UK) were assessed using two compatible enzymes: thermophilic Bst DNA polymerase large fragment (100% functional activity) (New England BioLabs, Hitchin, UK) and Tte UvrD helicase (100% functional activity) (New England BioLabs, Hitchin, UK). .. The assays were assessed based on HNB-visualized colour change and/or by using Agilent 2200 Tape Station (Agilent Technologies, Cheshire, UK) High Sensitivity D5000 DNA following manufacturer instructions.

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  • 95
    New England Biolabs tte uvrd helicase
    LAMP primer evaluation for amplification of the OsHV-1 plasmid (T = 65 °C, t = 10 min) and sensitivity with the addition of <t>Tte</t> <t>UvrD</t> <t>helicase</t> enzyme: ( A ) electropherogram comparison profiles of positive (OsHV-1 plasmid) and negative templates (NTC, UN_1 and AbHV) analysed using Tape Station Analysis Software A.02.02 (Agilent Technologies) for assessment of progressive increase of amplified LAMP product. Positive templates show a size shift of 350–5000 bp. ( B ) Colorimetric assessment of amplified LAMP products for positive (OsHV-1) (pink) and NTC (violet) templates using HNB.
    Tte Uvrd Helicase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tte uvrd helicase/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tte uvrd helicase - by Bioz Stars, 2021-06
    95/100 stars
      Buy from Supplier

    Image Search Results


    LAMP primer evaluation for amplification of the OsHV-1 plasmid (T = 65 °C, t = 10 min) and sensitivity with the addition of Tte UvrD helicase enzyme: ( A ) electropherogram comparison profiles of positive (OsHV-1 plasmid) and negative templates (NTC, UN_1 and AbHV) analysed using Tape Station Analysis Software A.02.02 (Agilent Technologies) for assessment of progressive increase of amplified LAMP product. Positive templates show a size shift of 350–5000 bp. ( B ) Colorimetric assessment of amplified LAMP products for positive (OsHV-1) (pink) and NTC (violet) templates using HNB.

    Journal: International Journal of Molecular Sciences

    Article Title: A Single-Tube HNB-Based Loop-Mediated Isothermal Amplification for the Robust Detection of the Ostreid herpesvirus 1

    doi: 10.3390/ijms21186605

    Figure Lengend Snippet: LAMP primer evaluation for amplification of the OsHV-1 plasmid (T = 65 °C, t = 10 min) and sensitivity with the addition of Tte UvrD helicase enzyme: ( A ) electropherogram comparison profiles of positive (OsHV-1 plasmid) and negative templates (NTC, UN_1 and AbHV) analysed using Tape Station Analysis Software A.02.02 (Agilent Technologies) for assessment of progressive increase of amplified LAMP product. Positive templates show a size shift of 350–5000 bp. ( B ) Colorimetric assessment of amplified LAMP products for positive (OsHV-1) (pink) and NTC (violet) templates using HNB.

    Article Snippet: Two different protocols based on ThermoPol Reaction Buffer Pack (New England BioLabs, Hitchin, UK) were assessed using two compatible enzymes: thermophilic Bst DNA polymerase large fragment (100% functional activity) (New England BioLabs, Hitchin, UK) and Tte UvrD helicase (100% functional activity) (New England BioLabs, Hitchin, UK).

    Techniques: Amplification, Plasmid Preparation, Software