nuclease p1  (New England Biolabs)


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    Name:
    Nuclease P1
    Description:
    Nuclease P1 is a zinc dependent single strand specific nuclease which hydrolyzes 3 5 phosphodiester bonds in RNA and ssDNA with no base specificity Nuclease P1 also exhibits 3 phosphomonoesterase activity Although a single strand specific nuclease Nuclease P1 does display some activity toward double stranded DNA dsDNA in Nuclease P1 Reaction Buffer If preferentially degrading single stranded nucleic acids ssDNA or RNA in the presence of dsDNA we recommend using Nuclease P1 in 1X NEBuffer 1 1 to limit activity on dsDNA while maintaining single strand nuclease activity
    Catalog Number:
    m0660s
    Price:
    51
    Size:
    10 000 units
    Category:
    Exonucleases
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    Structured Review

    New England Biolabs nuclease p1
    Nuclease P1
    Nuclease P1 is a zinc dependent single strand specific nuclease which hydrolyzes 3 5 phosphodiester bonds in RNA and ssDNA with no base specificity Nuclease P1 also exhibits 3 phosphomonoesterase activity Although a single strand specific nuclease Nuclease P1 does display some activity toward double stranded DNA dsDNA in Nuclease P1 Reaction Buffer If preferentially degrading single stranded nucleic acids ssDNA or RNA in the presence of dsDNA we recommend using Nuclease P1 in 1X NEBuffer 1 1 to limit activity on dsDNA while maintaining single strand nuclease activity
    https://www.bioz.com/result/nuclease p1/product/New England Biolabs
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    nuclease p1 - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Ribosome Profiling in Archaea Reveals Leaderless Translation, Novel Translational Initiation Sites, and Ribosome Pausing at Single Codon Resolution
    Article Snippet: .. After nuclease digestion, samples were loaded onto 10-50% sucrose gradients and RNA was isolated from monosome fractions as described above. .. Library preparation was performed as previously described ( ).

    Incubation:

    Article Title: Targeted mRNA Oxidation Regulates Sunflower Seed Dormancy Alleviation during Dry After-Ripening [C]Targeted mRNA Oxidation Regulates Sunflower Seed Dormancy Alleviation during Dry After-Ripening [C] [W]
    Article Snippet: .. Ten micrograms of mRNA were incubated for 2 h at 37°C with 10 units of nuclease P1 (New England Biolabs) in 20 mM sodium acetate buffer, pH 5.2, following by 1 h incubation at 37°C with 10 units of shrimp alkaline phosphatase (Promega) in 100 mM Tris-HCl, pH 7.5. .. The reaction mixture was then centrifuged (2 min, 13,000 g , 4°C), and 50 μL of supernatant was used for 8-OHG determination with competitive ELISA assay according to the manufacturer's instructions.

    Article Title: Maximizing the ovarian reserve in mice by evading LINE-1 genotoxicity
    Article Snippet: .. P1 nuclease (NEB, cat #M0660S) reactions were performed in 10 µL volume, incubated at 37 °C for 30 min followed by inactivation at 75 °C for 10 min. dsDNase (Thermofisher, cat #EN0771) reactions were performed in 20 µL volume, incubated at 37 °C for 10 min followed by inactivation at 55 °C for 5 min with addition of 10 mM DTT. ..

    other:

    Article Title: Sequence-dependent formation of intrastrand crosslink products from the UVB irradiation of duplex DNA containing a 5-bromo-2?-deoxyuridine or 5-bromo-2?-deoxycytidine
    Article Snippet: Calf intestinal alkaline phosphatase and nuclease P1 were from New England Biolabs (Beverly, MA) and US Biological (Swampscott, MA), respectively.

    Article Title: Sequence-dependent formation of intrastrand crosslink products from the UVB irradiation of duplex DNA containing a 5-bromo-2?-deoxyuridine or 5-bromo-2?-deoxycytidine
    Article Snippet: Five units of nuclease P1, 0.005 U of calf spleen phosphodiesterase, 10 μL of 300 mM sodium acetate (pH 5.0), and water were added to make the final volume of the solution 250 μL.

    Article Title: Paper-based nuclease protection assay with on-chip sample pretreatment for point-of-need nucleic acid detection.
    Article Snippet: P1 nuclease (from Penicillium citrinum) and 10× P1 nuclease reaction buffer were purchased from New England Biolabs, Inc. (Ipswich, MA).

    Article Title: CRISPR-Cas12a exploits R-loop asymmetry to form double-strand breaks
    Article Snippet: To generate a single-nucleotide ladder, the same radiolabeled DNA oligonucleotides were separately digested with nuclease P1, DNase I, trans -active AsCas12a, and T5 exonuclease (which all leave 5′ phosphate and 3′-OH on their cleavage products, chemically equivalent to products of Cas12a cis cleavage), and products were pooled at a 1:1:1:1 ratio (T5 exonuclease was not used for 5′-radiolabeled oligonucleotides) prior to loading on the gel.

    Article Title: An aminoacyl-tRNA synthetase that specifically activates pyrrolysine
    Article Snippet: Mature tRNAPyl (1 μg) was hydrolyzed to nucleosides by using nuclease P1, phosphodiesterase I, and Antarctic phosphatase (New England Biolabs) as described in ref. .

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  • 99
    New England Biolabs nuclease p1
    Nuclease P1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nuclease p1/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nuclease p1 - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

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