nuclease p1  (New England Biolabs)


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    Name:
    Nuclease P1
    Description:
    Nuclease P1 is a zinc dependent single strand specific nuclease which hydrolyzes 3 5 phosphodiester bonds in RNA and ssDNA with no base specificity Nuclease P1 also exhibits 3 phosphomonoesterase activity Although a single strand specific nuclease Nuclease P1 does display some activity toward double stranded DNA dsDNA in Nuclease P1 Reaction Buffer If preferentially degrading single stranded nucleic acids ssDNA or RNA in the presence of dsDNA we recommend using Nuclease P1 in 1X NEBuffer 1 1 to limit activity on dsDNA while maintaining single strand nuclease activity
    Catalog Number:
    m0660s
    Price:
    50
    Size:
    10 000 units
    Category:
    Exonucleases
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    Structured Review

    New England Biolabs nuclease p1
    Nuclease P1
    Nuclease P1 is a zinc dependent single strand specific nuclease which hydrolyzes 3 5 phosphodiester bonds in RNA and ssDNA with no base specificity Nuclease P1 also exhibits 3 phosphomonoesterase activity Although a single strand specific nuclease Nuclease P1 does display some activity toward double stranded DNA dsDNA in Nuclease P1 Reaction Buffer If preferentially degrading single stranded nucleic acids ssDNA or RNA in the presence of dsDNA we recommend using Nuclease P1 in 1X NEBuffer 1 1 to limit activity on dsDNA while maintaining single strand nuclease activity
    https://www.bioz.com/result/nuclease p1/product/New England Biolabs
    Average 88 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    nuclease p1 - by Bioz Stars, 2020-01
    88/100 stars

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    DNA Synthesis:

    Article Title: Sequence-dependent formation of intrastrand crosslink products from the UVB irradiation of duplex DNA containing a 5-bromo-2?-deoxyuridine or 5-bromo-2?-deoxycytidine
    Article Snippet: Calf intestinal alkaline phosphatase and nuclease P1 were from New England Biolabs (Beverly, MA) and US Biological (Swampscott, MA), respectively. .. The reagents used for solid-phase DNA synthesis were purchased from Glen Research Inc. (Sterling, VA).

    Positive Control:

    Article Title: A truncated aminoacyl-tRNA synthetase modifies RNA
    Article Snippet: After aminoacylation with YadB or AspRS (positive control), half of the reaction was deacylated with 0.2 mM Tris-acetate, pH 9.0. .. Digestion of tRNA to Nucleosides. tRNA was hydrolyzed to nucleosides by using nuclease P1 and Antarctic (acid) phosphatase (New England BioLabs); enzyme amounts are per 20 μg tRNA.

    Synthesized:

    Article Title: Sequence-dependent formation of intrastrand crosslink products from the UVB irradiation of duplex DNA containing a 5-bromo-2?-deoxyuridine or 5-bromo-2?-deoxycytidine
    Article Snippet: Calf intestinal alkaline phosphatase and nuclease P1 were from New England Biolabs (Beverly, MA) and US Biological (Swampscott, MA), respectively. .. Dinucleoside monophosphates d(GBr U) and d(ABr U) were synthesized following the previously reported procedures for the preparations of other dinucleoside monophosphates (Supplementary and ) ( , ).

    Article Title: The Rabies Virus L Protein Catalyzes mRNA Capping with GDP Polyribonucleotidyltransferase Activity
    Article Snippet: RNA Capping and GTP Hydrolysis Assays Five-nucleotide oligo-RNA substrates with different sequences were synthesized with T7 RNA polymerase using partially double-stranded oligo-DNAs as templates as described previously [ ]. .. Calf intestine alkaline phosphatase and nuclease P1 -resistant products were analyzed together with standard nucleotides (GMP, GDP, GTP, G(5′)ppp(5′)A (New England Biolabs, Ipswich, MA, USA), and/or G(5′)ppp(5′)G (New England Biolabs)) by thin layer chromatography on a polyethyleneimine-cellulose plate (EMD Millipore, Billerica, MA, USA) (PEI-cellulose TLC) as described [ ].

    Enzyme-linked Immunosorbent Assay:

    Article Title: Targeted mRNA Oxidation Regulates Sunflower Seed Dormancy Alleviation during Dry After-Ripening [C]Targeted mRNA Oxidation Regulates Sunflower Seed Dormancy Alleviation during Dry After-Ripening [C] [W]
    Article Snippet: 8-OHG was quantified in mRNA samples using the OxiSelect Oxidative RNA Damage ELISA kit (Cell Biolabs) with minor modifications. .. Ten micrograms of mRNA were incubated for 2 h at 37°C with 10 units of nuclease P1 (New England Biolabs) in 20 mM sodium acetate buffer, pH 5.2, following by 1 h incubation at 37°C with 10 units of shrimp alkaline phosphatase (Promega) in 100 mM Tris-HCl, pH 7.5.

    Article Title: Mitochondrial ROS regulate oxidative damage and mitophagy but not age-related muscle fiber atrophy
    Article Snippet: DNA samples were digested to nucleosides by incubating the denatured DNA with nuclease P1 (5U) for 2 h at 37 °C in sodium acetate (20 mM) pH 5.2, followed with treatment of alkaline phosphatase (5U, New England Biolabs) for 1 h at 37 °C in Tris (100 mM), pH 7.5. .. The reaction mixture was centrifuged for 5 min at 6,000 g and the supernatant was used for the 8-OHdG DNA damage ELISA kit (Cell Biolabs) according to supplier’s protocol.

    Incubation:

    Article Title: A truncated aminoacyl-tRNA synthetase modifies RNA
    Article Snippet: The reaction was stopped by adding 50 mM glucose and incubated at 0°C for 1.5 h. The tRNA was precipitated and further processed as described ( ). tRNAs were detected by Northern blotting. .. Digestion of tRNA to Nucleosides. tRNA was hydrolyzed to nucleosides by using nuclease P1 and Antarctic (acid) phosphatase (New England BioLabs); enzyme amounts are per 20 μg tRNA.

    Article Title: Targeted mRNA Oxidation Regulates Sunflower Seed Dormancy Alleviation during Dry After-Ripening [C]Targeted mRNA Oxidation Regulates Sunflower Seed Dormancy Alleviation during Dry After-Ripening [C] [W]
    Article Snippet: .. Ten micrograms of mRNA were incubated for 2 h at 37°C with 10 units of nuclease P1 (New England Biolabs) in 20 mM sodium acetate buffer, pH 5.2, following by 1 h incubation at 37°C with 10 units of shrimp alkaline phosphatase (Promega) in 100 mM Tris-HCl, pH 7.5. .. The reaction mixture was then centrifuged (2 min, 13,000 g , 4°C), and 50 μL of supernatant was used for 8-OHG determination with competitive ELISA assay according to the manufacturer's instructions.

    Article Title: An aminoacyl-tRNA synthetase that specifically activates pyrrolysine
    Article Snippet: Mature tRNAPyl (1 μg) was hydrolyzed to nucleosides by using nuclease P1, phosphodiesterase I, and Antarctic phosphatase (New England Biolabs) as described in ref. . .. Ten picomoles of tRNAPyl in 10 mM Tris/1 mM EDTA (pH 7) was totally digested to Gp-terminated oligonucleotides by using 1,000 units of RNase T1 (Ambion, Austin, TX) in a total volume of 6 μl; the solution was incubated for 30 min at 37°C.

    Article Title: The m6A Reader ECT2 Controls Trichome Morphology by Affecting mRNA Stability in Arabidopsis [OPEN]
    Article Snippet: RNA (100 ng) was digested with 1 unit of Nuclease P1 in 50 μL of buffer containing 10% 0.1 M ammonium acetate (pH 5.3) at 42°C for more than 3 h, followed by the addition of 1 unit of shrimp alkaline phosphatase (NEB) and 10% Cutsmart buffer. .. The mixture was incubated at 37°C for an additional 3 h. The samples were then centrifuged at 15,000 rpm for 30 min and the aqueous phase was injected into an LC-MS/MS system.

    Article Title: R-2HG Exhibits Anti-tumor Activity by Targeting FTO/m6A/MYC/CEBPA Signaling
    Article Snippet: 100 mM ammonium bicarbonate and 1 unit of alkaline phosphatase (Sigma, P5931) were then added to the reaction for another 2 hours incubation at 37°C. .. To monitor changes in mRNA cap m6 Am , purified mRNA was digested with RNA 5′ pyrophosphohydrolase (RppH; NEB, M0356S) prior to digestion with nuclease P1. mRNA was digested in 1 × NEB buffer 2 with 2 μL of RppH in a 30 μL volume for 2 hours at 37°C.

    Mass Spectrometry:

    Article Title: A truncated aminoacyl-tRNA synthetase modifies RNA
    Article Snippet: Digestion of tRNA to Nucleosides. tRNA was hydrolyzed to nucleosides by using nuclease P1 and Antarctic (acid) phosphatase (New England BioLabs); enzyme amounts are per 20 μg tRNA. .. Digests of bulk W3110 tRNA were analyzed on an HP1090 liquid chromatograph coupled directly to a Micromass Quattro II mass spectrometer.

    Article Title: An aminoacyl-tRNA synthetase that specifically activates pyrrolysine
    Article Snippet: Mature tRNAPyl (1 μg) was hydrolyzed to nucleosides by using nuclease P1, phosphodiesterase I, and Antarctic phosphatase (New England Biolabs) as described in ref. . .. Each of the tRNA digests was analyzed directly by MS, without any cleanup.

    Article Title: The m6A Reader ECT2 Controls Trichome Morphology by Affecting mRNA Stability in Arabidopsis [OPEN]
    Article Snippet: RNA (100 ng) was digested with 1 unit of Nuclease P1 in 50 μL of buffer containing 10% 0.1 M ammonium acetate (pH 5.3) at 42°C for more than 3 h, followed by the addition of 1 unit of shrimp alkaline phosphatase (NEB) and 10% Cutsmart buffer. .. Nucleosides were separated using a UPLC pump (Shimadzu) with a ZORBAX SB-Aq column (Agilent) and analyzed by MS/MS using a Triple Quad 5500 mass spectrometer (AB SCIEX) running in positive ion mode and the multiple reaction monitoring feature.

    Article Title: R-2HG Exhibits Anti-tumor Activity by Targeting FTO/m6A/MYC/CEBPA Signaling
    Article Snippet: To monitor changes in mRNA cap m6 Am , purified mRNA was digested with RNA 5′ pyrophosphohydrolase (RppH; NEB, M0356S) prior to digestion with nuclease P1. mRNA was digested in 1 × NEB buffer 2 with 2 μL of RppH in a 30 μL volume for 2 hours at 37°C. .. The sample was then filtered through a 0.22 μm syringe filter prior to injection on a C18 reverse phase column coupled to an Agilent 6410 triple-quadropole LC mass spectrometer in positive electrospray ionization mode.

    Hybridization:

    Article Title: A truncated aminoacyl-tRNA synthetase modifies RNA
    Article Snippet: Membranes were then baked at 72°C for 2 h, and tRNAs detected by hybridization with a 5′-[32 P]-oligo-deoxyribonucleotide probe complementary to nucleotides 32–51 of tRNAAsp . .. Digestion of tRNA to Nucleosides. tRNA was hydrolyzed to nucleosides by using nuclease P1 and Antarctic (acid) phosphatase (New England BioLabs); enzyme amounts are per 20 μg tRNA.

    Article Title: An aminoacyl-tRNA synthetase that specifically activates pyrrolysine
    Article Snippet: The tRNAs were detected by hybridization with a 5′ [32 P]-labeled oligodeoxyribonucleotide probe. .. Mature tRNAPyl (1 μg) was hydrolyzed to nucleosides by using nuclease P1, phosphodiesterase I, and Antarctic phosphatase (New England Biolabs) as described in ref. .

    Northern Blot:

    Article Title: A truncated aminoacyl-tRNA synthetase modifies RNA
    Article Snippet: The reaction was stopped by adding 50 mM glucose and incubated at 0°C for 1.5 h. The tRNA was precipitated and further processed as described ( ). tRNAs were detected by Northern blotting. .. Digestion of tRNA to Nucleosides. tRNA was hydrolyzed to nucleosides by using nuclease P1 and Antarctic (acid) phosphatase (New England BioLabs); enzyme amounts are per 20 μg tRNA.

    other:

    Article Title: Sequence-dependent formation of intrastrand crosslink products from the UVB irradiation of duplex DNA containing a 5-bromo-2?-deoxyuridine or 5-bromo-2?-deoxycytidine
    Article Snippet: Five units of nuclease P1, 0.005 U of calf spleen phosphodiesterase, 10 μL of 300 mM sodium acetate (pH 5.0), and water were added to make the final volume of the solution 250 μL.

    Article Title: Two discriminatory binding sites in the Escherichia coli replication origin are required for DNA strand opening by initiator DnaA-ATP
    Article Snippet: The resulting single-stranded DNA is susceptible to cleavage by P1 endonuclease ( , , , ).

    Article Title: Two discriminatory binding sites in the Escherichia coli replication origin are required for DNA strand opening by initiator DnaA-ATP
    Article Snippet: On supercoiled templates, P1 endonuclease makes opposing cuts on both strands of the open complex, resulting in linearization of the molecule.

    Tandem Mass Spectroscopy:

    Article Title: The m6A Reader ECT2 Controls Trichome Morphology by Affecting mRNA Stability in Arabidopsis [OPEN]
    Article Snippet: RNA (100 ng) was digested with 1 unit of Nuclease P1 in 50 μL of buffer containing 10% 0.1 M ammonium acetate (pH 5.3) at 42°C for more than 3 h, followed by the addition of 1 unit of shrimp alkaline phosphatase (NEB) and 10% Cutsmart buffer. .. Nucleosides were separated using a UPLC pump (Shimadzu) with a ZORBAX SB-Aq column (Agilent) and analyzed by MS/MS using a Triple Quad 5500 mass spectrometer (AB SCIEX) running in positive ion mode and the multiple reaction monitoring feature.

    Injection:

    Article Title: The m6A Reader ECT2 Controls Trichome Morphology by Affecting mRNA Stability in Arabidopsis [OPEN]
    Article Snippet: RNA (100 ng) was digested with 1 unit of Nuclease P1 in 50 μL of buffer containing 10% 0.1 M ammonium acetate (pH 5.3) at 42°C for more than 3 h, followed by the addition of 1 unit of shrimp alkaline phosphatase (NEB) and 10% Cutsmart buffer. .. The mixture was incubated at 37°C for an additional 3 h. The samples were then centrifuged at 15,000 rpm for 30 min and the aqueous phase was injected into an LC-MS/MS system.

    Article Title: R-2HG Exhibits Anti-tumor Activity by Targeting FTO/m6A/MYC/CEBPA Signaling
    Article Snippet: To monitor changes in mRNA cap m6 Am , purified mRNA was digested with RNA 5′ pyrophosphohydrolase (RppH; NEB, M0356S) prior to digestion with nuclease P1. mRNA was digested in 1 × NEB buffer 2 with 2 μL of RppH in a 30 μL volume for 2 hours at 37°C. .. The sample was then filtered through a 0.22 μm syringe filter prior to injection on a C18 reverse phase column coupled to an Agilent 6410 triple-quadropole LC mass spectrometer in positive electrospray ionization mode.

    Binding Assay:

    Article Title: Two discriminatory binding sites in the Escherichia coli replication origin are required for DNA strand opening by initiator DnaA-ATP
    Article Snippet: .. To test the effect of binding-defective I sites on oriC function, DnaA-ATP-catalyzed strand opening was detected by using P1 endonuclease. ..

    Mutagenesis:

    Article Title: The Rabies Virus L Protein Catalyzes mRNA Capping with GDP Polyribonucleotidyltransferase Activity
    Article Snippet: In vitro RNA capping was carried out with the recombinant RABV L protein (0.1 μg, WT or mutant) using 5 μM oligo-RNA and 0.25 μM [α-32 P]GDP or [α-32 P]GTP (1.5–2 × 103 cpm/fmol) as substrates according to the method for the VSV L protein [ ]. .. Calf intestine alkaline phosphatase and nuclease P1 -resistant products were analyzed together with standard nucleotides (GMP, GDP, GTP, G(5′)ppp(5′)A (New England Biolabs, Ipswich, MA, USA), and/or G(5′)ppp(5′)G (New England Biolabs)) by thin layer chromatography on a polyethyleneimine-cellulose plate (EMD Millipore, Billerica, MA, USA) (PEI-cellulose TLC) as described [ ].

    Isolation:

    Article Title: R-2HG Exhibits Anti-tumor Activity by Targeting FTO/m6A/MYC/CEBPA Signaling
    Article Snippet: Total RNA was isolated from Trizol and then polyadenylated RNA was extracted using a GenElute mRNA miniprep kit (Sigma) or a Dynabeads mRNA DIRECT kit (Thermo Fisher). .. To monitor changes in mRNA cap m6 Am , purified mRNA was digested with RNA 5′ pyrophosphohydrolase (RppH; NEB, M0356S) prior to digestion with nuclease P1. mRNA was digested in 1 × NEB buffer 2 with 2 μL of RppH in a 30 μL volume for 2 hours at 37°C.

    Purification:

    Article Title: Targeted mRNA Oxidation Regulates Sunflower Seed Dormancy Alleviation during Dry After-Ripening [C]Targeted mRNA Oxidation Regulates Sunflower Seed Dormancy Alleviation during Dry After-Ripening [C] [W]
    Article Snippet: Purified mRNA from different samples was digested to nucleosides using nuclease P1 and alkaline phosphatase treatment. .. Ten micrograms of mRNA were incubated for 2 h at 37°C with 10 units of nuclease P1 (New England Biolabs) in 20 mM sodium acetate buffer, pH 5.2, following by 1 h incubation at 37°C with 10 units of shrimp alkaline phosphatase (Promega) in 100 mM Tris-HCl, pH 7.5.

    Article Title: R-2HG Exhibits Anti-tumor Activity by Targeting FTO/m6A/MYC/CEBPA Signaling
    Article Snippet: .. To monitor changes in mRNA cap m6 Am , purified mRNA was digested with RNA 5′ pyrophosphohydrolase (RppH; NEB, M0356S) prior to digestion with nuclease P1. mRNA was digested in 1 × NEB buffer 2 with 2 μL of RppH in a 30 μL volume for 2 hours at 37°C. .. 2 μL of fresh RppH diluted in 10 μL total volume of 1 × NEB buffer 2 was added to each reaction and incubated for another 2 hours at 37°C.

    Competitive ELISA:

    Article Title: Targeted mRNA Oxidation Regulates Sunflower Seed Dormancy Alleviation during Dry After-Ripening [C]Targeted mRNA Oxidation Regulates Sunflower Seed Dormancy Alleviation during Dry After-Ripening [C] [W]
    Article Snippet: Ten micrograms of mRNA were incubated for 2 h at 37°C with 10 units of nuclease P1 (New England Biolabs) in 20 mM sodium acetate buffer, pH 5.2, following by 1 h incubation at 37°C with 10 units of shrimp alkaline phosphatase (Promega) in 100 mM Tris-HCl, pH 7.5. .. The reaction mixture was then centrifuged (2 min, 13,000 g , 4°C), and 50 μL of supernatant was used for 8-OHG determination with competitive ELISA assay according to the manufacturer's instructions.

    De-Phosphorylation Assay:

    Article Title: Structure elucidation of colibactin and its DNA cross-links
    Article Snippet: Then 10 units/μg of Nuclease P1 (New England Biolabs) was added to the digestion mix, and the second step digestion lasted at 37°C for 1 hour. .. Finally, 1 unit/μg DNA of Quick Dephosphorylation Kit (New England Biolabs) was added to the digestion mix, and the third step digestion lasted at 37°C for 30 min.

    Liquid Chromatography:

    Article Title: A truncated aminoacyl-tRNA synthetase modifies RNA
    Article Snippet: Digestion of tRNA to Nucleosides. tRNA was hydrolyzed to nucleosides by using nuclease P1 and Antarctic (acid) phosphatase (New England BioLabs); enzyme amounts are per 20 μg tRNA. .. After incubation for 2 h at 37°C, 1 unit of acid phosphatase (1 unit/μl) was added, and the reaction was continued a further 2 h. Liquid Chromatography / Electrospray Ionization-Mass Spectrometry of Digested tRNA. tRNA hydrolyzates were analyzed directly, without cleanup.

    Article Title: An aminoacyl-tRNA synthetase that specifically activates pyrrolysine
    Article Snippet: Mature tRNAPyl (1 μg) was hydrolyzed to nucleosides by using nuclease P1, phosphodiesterase I, and Antarctic phosphatase (New England Biolabs) as described in ref. . .. Liquid Chromatography (LC) / ESI MS. Amino acid analysis.

    Plasmid Preparation:

    Article Title: Two discriminatory binding sites in the Escherichia coli replication origin are required for DNA strand opening by initiator DnaA-ATP
    Article Snippet: .. Measurement of plasmid linearization by P1 endonuclease reveals the presence of single-stranded regions, but does not identify the location of the unwound regions on the plasmid. ..

    Hydrolysis Assay:

    Article Title: The Rabies Virus L Protein Catalyzes mRNA Capping with GDP Polyribonucleotidyltransferase Activity
    Article Snippet: Calf intestine alkaline phosphatase and nuclease P1 -resistant products were analyzed together with standard nucleotides (GMP, GDP, GTP, G(5′)ppp(5′)A (New England Biolabs, Ipswich, MA, USA), and/or G(5′)ppp(5′)G (New England Biolabs)) by thin layer chromatography on a polyethyleneimine-cellulose plate (EMD Millipore, Billerica, MA, USA) (PEI-cellulose TLC) as described [ ]. .. The GTP hydrolysis assay was performed with 0.1 or 0.2 μg of the recombinant RABV L protein using 0.25 μM [γ-32 P]GTP (2 × 104 cpm/pmol) as described [ , ].

    In Vitro:

    Article Title: Multiple nucleotide preferences determine cleavage site recognition by the HIV-1 and M-MuLV RNases H
    Article Snippet: DNA oligonucleotides were purchased from Eurofins MWG Operon, Invitrogen, and Bioneer, Inc. RNAs were made by in vitro transcription with the MEGAshortscript™ Kit from Ambion. .. To generate RNA ladders for mapping cleavage sites, nuclease P1 (cleaves at all positions) and RNase T1 (cleaves 3′ of G positions) were obtained from New England Biolabs and BRL (now Invitrogen), respectively.

    Article Title: The Rabies Virus L Protein Catalyzes mRNA Capping with GDP Polyribonucleotidyltransferase Activity
    Article Snippet: In vitro RNA capping was carried out with the recombinant RABV L protein (0.1 μg, WT or mutant) using 5 μM oligo-RNA and 0.25 μM [α-32 P]GDP or [α-32 P]GTP (1.5–2 × 103 cpm/fmol) as substrates according to the method for the VSV L protein [ ]. .. Calf intestine alkaline phosphatase and nuclease P1 -resistant products were analyzed together with standard nucleotides (GMP, GDP, GTP, G(5′)ppp(5′)A (New England Biolabs, Ipswich, MA, USA), and/or G(5′)ppp(5′)G (New England Biolabs)) by thin layer chromatography on a polyethyleneimine-cellulose plate (EMD Millipore, Billerica, MA, USA) (PEI-cellulose TLC) as described [ ].

    Ethanol Precipitation:

    Article Title: A truncated aminoacyl-tRNA synthetase modifies RNA
    Article Snippet: After ethanol precipitation, the RNA of both samples was treated in the dark with 2.5 mM NaIO4 in 50 mM sodium acetate, pH 5.0, for 1 h at 37°C. .. Digestion of tRNA to Nucleosides. tRNA was hydrolyzed to nucleosides by using nuclease P1 and Antarctic (acid) phosphatase (New England BioLabs); enzyme amounts are per 20 μg tRNA.

    Quantitation Assay:

    Article Title: R-2HG Exhibits Anti-tumor Activity by Targeting FTO/m6A/MYC/CEBPA Signaling
    Article Snippet: Paragraph title: Quantitation of m6 A and m6 Am levels by LC-MS/MS ... To monitor changes in mRNA cap m6 Am , purified mRNA was digested with RNA 5′ pyrophosphohydrolase (RppH; NEB, M0356S) prior to digestion with nuclease P1. mRNA was digested in 1 × NEB buffer 2 with 2 μL of RppH in a 30 μL volume for 2 hours at 37°C.

    Thin Layer Chromatography:

    Article Title: The Rabies Virus L Protein Catalyzes mRNA Capping with GDP Polyribonucleotidyltransferase Activity
    Article Snippet: .. Calf intestine alkaline phosphatase and nuclease P1 -resistant products were analyzed together with standard nucleotides (GMP, GDP, GTP, G(5′)ppp(5′)A (New England Biolabs, Ipswich, MA, USA), and/or G(5′)ppp(5′)G (New England Biolabs)) by thin layer chromatography on a polyethyleneimine-cellulose plate (EMD Millipore, Billerica, MA, USA) (PEI-cellulose TLC) as described [ ]. .. The GTP hydrolysis assay was performed with 0.1 or 0.2 μg of the recombinant RABV L protein using 0.25 μM [γ-32 P]GTP (2 × 104 cpm/pmol) as described [ , ].

    Recombinant:

    Article Title: Multiple nucleotide preferences determine cleavage site recognition by the HIV-1 and M-MuLV RNases H
    Article Snippet: The recombinant RNase H domain of M-MuLV reverse transcriptase was prepared as described previously . .. To generate RNA ladders for mapping cleavage sites, nuclease P1 (cleaves at all positions) and RNase T1 (cleaves 3′ of G positions) were obtained from New England Biolabs and BRL (now Invitrogen), respectively.

    Article Title: The Rabies Virus L Protein Catalyzes mRNA Capping with GDP Polyribonucleotidyltransferase Activity
    Article Snippet: In vitro RNA capping was carried out with the recombinant RABV L protein (0.1 μg, WT or mutant) using 5 μM oligo-RNA and 0.25 μM [α-32 P]GDP or [α-32 P]GTP (1.5–2 × 103 cpm/fmol) as substrates according to the method for the VSV L protein [ ]. .. Calf intestine alkaline phosphatase and nuclease P1 -resistant products were analyzed together with standard nucleotides (GMP, GDP, GTP, G(5′)ppp(5′)A (New England Biolabs, Ipswich, MA, USA), and/or G(5′)ppp(5′)G (New England Biolabs)) by thin layer chromatography on a polyethyleneimine-cellulose plate (EMD Millipore, Billerica, MA, USA) (PEI-cellulose TLC) as described [ ].

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  • 88
    New England Biolabs nuclease p1
    Nuclease P1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nuclease p1/product/New England Biolabs
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nuclease p1 - by Bioz Stars, 2020-01
    88/100 stars
      Buy from Supplier

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