nucleoside digestion mix  (New England Biolabs)


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  • 98
    Name:
    Nucleoside Digestion Mix 50 rxns
    Description:
    Nucleoside Digestion Mix 50 rxns
    Catalog Number:
    m0649s
    Price:
    119
    Size:
    50 rxns
    Category:
    Exonucleases
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    Structured Review

    New England Biolabs nucleoside digestion mix
    Nucleoside Digestion Mix 50 rxns
    Nucleoside Digestion Mix 50 rxns
    https://www.bioz.com/result/nucleoside digestion mix/product/New England Biolabs
    Average 98 stars, based on 165 article reviews
    Price from $9.99 to $1999.99
    nucleoside digestion mix - by Bioz Stars, 2020-09
    98/100 stars

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    Related Articles

    Methylation:

    Article Title: The yeast scavenger decapping enzyme DcpS and its application for in vitro RNA recapping
    Article Snippet: .. Complete methylation was verified by digestion of RNA with the Nucleoside Digestion Mix (NEB, M0649) and LC-MS analysis. .. In vitro cap specificity assay of yDcpS with 30 different cap structures The reaction conditions for decapping consisted of 100 nM 5′-capped 25mer RNA and 0, 0.3, 0.9, 2.7, 8 or 25 µM DcpS in a 10 µL reaction volume in 10 mM Bis-Tris pH 6.5 and 1 mM EDTA.

    Purification:

    Article Title: Single species RNA purification with DNA nanoswitches
    Article Snippet: .. After the purification of the RNA with m4,4 C modification, we first dried the sample in a universal vacuum system (Savant UVS 400) and then resuspended in RNase-free water and digested the RNA into nucleotides by Nucleoside Digestion Mix (NEB, M0649S) in 15 μl volume and used 10 μl for the test. .. The UHPLC-MS/MS analysis was accomplished on a Waters XEVO TQ-S™ (Waters Corporation, USA) triple quadruple tandem mass spectrometer equipped with an electrospray source (ESI) maintained at 150 °C and a capillary voltage of 1 kV.

    Concentration Assay:

    Article Title: Identification and biosynthesis of thymidine hypermodifications in the genomic DNA of widespread bacterial viruses
    Article Snippet: .. Dialyzed phage ViI DNA (100 mg) was digested at 0.1 mg/mL final concentration to nucleosides by treatment overnight at 37 °C with the Nucleoside Digestion Mix in 1 mL of 1× Nucleoside Digestion Mix buffer (NEB). .. After passage through a 3-kDa molecular mass cutoff Vivaspin 20 spin column (GE Healthcare Bio-Sciences) to remove proteins, the flow through was collected and passed through a 0.22-µm membrane (Corning) to remove any particles before HPLC purification.

    Article Title: Identification and biosynthesis of thymidine hypermodifications in the genomic DNA of widespread bacterial viruses
    Article Snippet: .. Five micrograms of virion DNA from each of the phages 1.255.O, CBA6, CBA120, and M6 DNA were digested to nucleosides at 0.1–0.2 μg/μL final concentration by treatment overnight at 37 °C with 5 μL of the Nucleoside Digestion Mix in 1× Nucleoside Digestion Mix Reaction Buffer (NEB). .. The resulting DNA nucleoside mixtures were directly analyzed by reversed-phase LC/MS without additional purification.

    other:

    Article Title: DNA hydroxymethylation reveals transcription regulation networks and prognostic signatures in multiple myeloma
    Article Snippet: As heavy-atom-labeled internal standards, 1.28 pmol D3 -mC and 0.193 pmol D2 N2 -hmC in 6 µ L of milliQ-water were added to the solution, followed by 5 µ L of the Nucleoside Digestion Mix Reaction Buffer (10x), and 1 µ L of the Nucleoside Digestion Mix.

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: The yeast scavenger decapping enzyme DcpS and its application for in vitro RNA recapping
    Article Snippet: .. Complete methylation was verified by digestion of RNA with the Nucleoside Digestion Mix (NEB, M0649) and LC-MS analysis. .. In vitro cap specificity assay of yDcpS with 30 different cap structures The reaction conditions for decapping consisted of 100 nM 5′-capped 25mer RNA and 0, 0.3, 0.9, 2.7, 8 or 25 µM DcpS in a 10 µL reaction volume in 10 mM Bis-Tris pH 6.5 and 1 mM EDTA.

    Modification:

    Article Title: Single species RNA purification with DNA nanoswitches
    Article Snippet: .. After the purification of the RNA with m4,4 C modification, we first dried the sample in a universal vacuum system (Savant UVS 400) and then resuspended in RNase-free water and digested the RNA into nucleotides by Nucleoside Digestion Mix (NEB, M0649S) in 15 μl volume and used 10 μl for the test. .. The UHPLC-MS/MS analysis was accomplished on a Waters XEVO TQ-S™ (Waters Corporation, USA) triple quadruple tandem mass spectrometer equipped with an electrospray source (ESI) maintained at 150 °C and a capillary voltage of 1 kV.

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  • 98
    New England Biolabs nucleoside digestion mix
    Nucleoside Digestion Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nucleoside digestion mix/product/New England Biolabs
    Average 98 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    nucleoside digestion mix - by Bioz Stars, 2020-09
    98/100 stars
      Buy from Supplier

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