hifi taq dna ligase  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Name:
    HiFi Taq DNA Ligase
    Description:
    HiFi Taq DNA Ligase 50 rxns
    Catalog Number:
    m0647s
    Price:
    152
    Size:
    50 rxns
    Category:
    DNA Ligases
    Buy from Supplier


    Structured Review

    New England Biolabs hifi taq dna ligase
    HiFi Taq DNA Ligase
    HiFi Taq DNA Ligase 50 rxns
    https://www.bioz.com/result/hifi taq dna ligase/product/New England Biolabs
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    hifi taq dna ligase - by Bioz Stars, 2020-02
    93/100 stars

    Related Products / Commonly Used Together

    moligo probe
    10x hifi taq dna ligase reaction buffer

    Images

    Related Articles

    Clone Assay:

    Article Title: Viperin interaction with mitochondrial antiviral signaling protein (MAVS) limits viperin-mediated inhibition of the interferon response in macrophages
    Article Snippet: .. Viperin was cloned into pRetroX (Clontech) by PCR using Taq-HiFi (NEB) and digested with restriction enzymes (NEB), ligated using T4 DNA ligase (NEB) into the digested vector backbone, and transformed into Stbl3 cells (Thermo Fisher Scientific). pCMV-FLAG-MAVS was a kind gift from the laboratory of Dr. Akiko Iwasaki. pCMV-SPORT6-ΔtmMAVS was a kind gift from the laboratory of Dr. Yorgo Mordis. pcDNA3.1-MDA5 and pcDNA3.1-RIGI were a kind gift from Dr. Shu Zhu in the laboratory of Dr. Richard Flavell. .. Plasmids were purified using Qiagen miniprep kits or kits from Origene and Zymo Research.

    Multiplex Assay:

    Article Title: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay
    Article Snippet: Paragraph title: Multiplex oligonucleotide ligation ... The optimized ligation reaction mix combined 5 nM of each MOLigo probe (Table ) with 2.5 μl of 10X Hifi Taq DNA ligase reaction buffer, 0.5 μl of Hifi Taq DNA ligase (New England BioLabs, Massachusetts, USA), and 2.5 μl of template DNA (corresponds to ∼2.5 ng of isolated DNA).

    Amplification:

    Article Title: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay
    Article Snippet: Multiplex oligonucleotide ligation The multiplex ligation step was separated from PCR amplification in order to avoid the high background signal levels when combining both steps in one reaction . .. The optimized ligation reaction mix combined 5 nM of each MOLigo probe (Table ) with 2.5 μl of 10X Hifi Taq DNA ligase reaction buffer, 0.5 μl of Hifi Taq DNA ligase (New England BioLabs, Massachusetts, USA), and 2.5 μl of template DNA (corresponds to ∼2.5 ng of isolated DNA).

    Filtration:

    Article Title: The lyase activity of bifunctional DNA glycosylases and the 3′-diesterase activity of APE1 contribute to the repair of oxidized bases in nucleosomes
    Article Snippet: A second 136bp oligonucleotide, identical to the downstream sequence, was 5′ end labeled with 32 P. Both were annealed to a 184 NT compliment and ligated, using HIFI Taq ligase (NEB) at 40°C. .. DNA was then combined with histone octamers previously reconstituted from recombinant X. Lavis histones and purified by gel filtration ( , ).

    Ligation:

    Article Title: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay
    Article Snippet: .. This might reflect different requirements for optimal performance of each thermostable ligation enzyme; in the case of Hifi Taq DNA ligase, typical nick-ligation procedures should be performed at 60 °C. .. Therefore, a temperature range of about 59–60 °C fulfills the prerequisites for the best activity and fidelity of the enzyme based on oligo-template properties and ensures the reduction of errors caused by mismatched base pairs.

    Article Title: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay
    Article Snippet: .. The optimized ligation reaction mix combined 5 nM of each MOLigo probe (Table ) with 2.5 μl of 10X Hifi Taq DNA ligase reaction buffer, 0.5 μl of Hifi Taq DNA ligase (New England BioLabs, Massachusetts, USA), and 2.5 μl of template DNA (corresponds to ∼2.5 ng of isolated DNA). .. The reaction was brought to a final volume of 25 μl with PCR H2 O (Top-Bio, Czech Republic).

    Isolation:

    Article Title: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay
    Article Snippet: .. The optimized ligation reaction mix combined 5 nM of each MOLigo probe (Table ) with 2.5 μl of 10X Hifi Taq DNA ligase reaction buffer, 0.5 μl of Hifi Taq DNA ligase (New England BioLabs, Massachusetts, USA), and 2.5 μl of template DNA (corresponds to ∼2.5 ng of isolated DNA). .. The reaction was brought to a final volume of 25 μl with PCR H2 O (Top-Bio, Czech Republic).

    Labeling:

    Article Title: The lyase activity of bifunctional DNA glycosylases and the 3′-diesterase activity of APE1 contribute to the repair of oxidized bases in nucleosomes
    Article Snippet: .. A second 136bp oligonucleotide, identical to the downstream sequence, was 5′ end labeled with 32 P. Both were annealed to a 184 NT compliment and ligated, using HIFI Taq ligase (NEB) at 40°C. .. Full-length DNA products were purified using 8% polyacrylamide denaturing gels, then heated 95°C and annealed by slow cooling to room temp.

    Purification:

    Article Title: Viperin interaction with mitochondrial antiviral signaling protein (MAVS) limits viperin-mediated inhibition of the interferon response in macrophages
    Article Snippet: Viperin was cloned into pRetroX (Clontech) by PCR using Taq-HiFi (NEB) and digested with restriction enzymes (NEB), ligated using T4 DNA ligase (NEB) into the digested vector backbone, and transformed into Stbl3 cells (Thermo Fisher Scientific). pCMV-FLAG-MAVS was a kind gift from the laboratory of Dr. Akiko Iwasaki. pCMV-SPORT6-ΔtmMAVS was a kind gift from the laboratory of Dr. Yorgo Mordis. pcDNA3.1-MDA5 and pcDNA3.1-RIGI were a kind gift from Dr. Shu Zhu in the laboratory of Dr. Richard Flavell. .. Plasmids were purified using Qiagen miniprep kits or kits from Origene and Zymo Research.

    Article Title: The lyase activity of bifunctional DNA glycosylases and the 3′-diesterase activity of APE1 contribute to the repair of oxidized bases in nucleosomes
    Article Snippet: A second 136bp oligonucleotide, identical to the downstream sequence, was 5′ end labeled with 32 P. Both were annealed to a 184 NT compliment and ligated, using HIFI Taq ligase (NEB) at 40°C. .. Full-length DNA products were purified using 8% polyacrylamide denaturing gels, then heated 95°C and annealed by slow cooling to room temp.

    Polymerase Chain Reaction:

    Article Title: Viperin interaction with mitochondrial antiviral signaling protein (MAVS) limits viperin-mediated inhibition of the interferon response in macrophages
    Article Snippet: .. Viperin was cloned into pRetroX (Clontech) by PCR using Taq-HiFi (NEB) and digested with restriction enzymes (NEB), ligated using T4 DNA ligase (NEB) into the digested vector backbone, and transformed into Stbl3 cells (Thermo Fisher Scientific). pCMV-FLAG-MAVS was a kind gift from the laboratory of Dr. Akiko Iwasaki. pCMV-SPORT6-ΔtmMAVS was a kind gift from the laboratory of Dr. Yorgo Mordis. pcDNA3.1-MDA5 and pcDNA3.1-RIGI were a kind gift from Dr. Shu Zhu in the laboratory of Dr. Richard Flavell. .. Plasmids were purified using Qiagen miniprep kits or kits from Origene and Zymo Research.

    Article Title: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay
    Article Snippet: Multiplex oligonucleotide ligation The multiplex ligation step was separated from PCR amplification in order to avoid the high background signal levels when combining both steps in one reaction . .. The optimized ligation reaction mix combined 5 nM of each MOLigo probe (Table ) with 2.5 μl of 10X Hifi Taq DNA ligase reaction buffer, 0.5 μl of Hifi Taq DNA ligase (New England BioLabs, Massachusetts, USA), and 2.5 μl of template DNA (corresponds to ∼2.5 ng of isolated DNA).

    Generated:

    Article Title: The lyase activity of bifunctional DNA glycosylases and the 3′-diesterase activity of APE1 contribute to the repair of oxidized bases in nucleosomes
    Article Snippet: DNA substrates used in Figure were generated using the same Tg containing 48 bp oligonucleotide as above. .. A second 136bp oligonucleotide, identical to the downstream sequence, was 5′ end labeled with 32 P. Both were annealed to a 184 NT compliment and ligated, using HIFI Taq ligase (NEB) at 40°C.

    other:

    Article Title: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay
    Article Snippet: The best efficiency and lowest background noise was obtained with the Hifi Taq DNA Ligase (see Supplementary Table ), of which it has been claimed that it is the NAD+ -dependent DNA ligase with the highest fidelity currently available commercially .

    Article Title: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay
    Article Snippet: The highest fidelity was proven when using Hifi Taq DNA Ligase, which represents the state-of-the-art in the field of thermostable ligases; however, this novel ligase is a factor that significantly increases the cost of analysis.

    Sequencing:

    Article Title: The lyase activity of bifunctional DNA glycosylases and the 3′-diesterase activity of APE1 contribute to the repair of oxidized bases in nucleosomes
    Article Snippet: .. A second 136bp oligonucleotide, identical to the downstream sequence, was 5′ end labeled with 32 P. Both were annealed to a 184 NT compliment and ligated, using HIFI Taq ligase (NEB) at 40°C. .. Full-length DNA products were purified using 8% polyacrylamide denaturing gels, then heated 95°C and annealed by slow cooling to room temp.

    Transformation Assay:

    Article Title: Viperin interaction with mitochondrial antiviral signaling protein (MAVS) limits viperin-mediated inhibition of the interferon response in macrophages
    Article Snippet: .. Viperin was cloned into pRetroX (Clontech) by PCR using Taq-HiFi (NEB) and digested with restriction enzymes (NEB), ligated using T4 DNA ligase (NEB) into the digested vector backbone, and transformed into Stbl3 cells (Thermo Fisher Scientific). pCMV-FLAG-MAVS was a kind gift from the laboratory of Dr. Akiko Iwasaki. pCMV-SPORT6-ΔtmMAVS was a kind gift from the laboratory of Dr. Yorgo Mordis. pcDNA3.1-MDA5 and pcDNA3.1-RIGI were a kind gift from Dr. Shu Zhu in the laboratory of Dr. Richard Flavell. .. Plasmids were purified using Qiagen miniprep kits or kits from Origene and Zymo Research.

    Recombinant:

    Article Title: The lyase activity of bifunctional DNA glycosylases and the 3′-diesterase activity of APE1 contribute to the repair of oxidized bases in nucleosomes
    Article Snippet: A second 136bp oligonucleotide, identical to the downstream sequence, was 5′ end labeled with 32 P. Both were annealed to a 184 NT compliment and ligated, using HIFI Taq ligase (NEB) at 40°C. .. DNA was then combined with histone octamers previously reconstituted from recombinant X. Lavis histones and purified by gel filtration ( , ).

    Plasmid Preparation:

    Article Title: Viperin interaction with mitochondrial antiviral signaling protein (MAVS) limits viperin-mediated inhibition of the interferon response in macrophages
    Article Snippet: .. Viperin was cloned into pRetroX (Clontech) by PCR using Taq-HiFi (NEB) and digested with restriction enzymes (NEB), ligated using T4 DNA ligase (NEB) into the digested vector backbone, and transformed into Stbl3 cells (Thermo Fisher Scientific). pCMV-FLAG-MAVS was a kind gift from the laboratory of Dr. Akiko Iwasaki. pCMV-SPORT6-ΔtmMAVS was a kind gift from the laboratory of Dr. Yorgo Mordis. pcDNA3.1-MDA5 and pcDNA3.1-RIGI were a kind gift from Dr. Shu Zhu in the laboratory of Dr. Richard Flavell. .. Plasmids were purified using Qiagen miniprep kits or kits from Origene and Zymo Research.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    New England Biolabs hifi taq dna ligase
    Hifi Taq Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hifi taq dna ligase/product/New England Biolabs
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    hifi taq dna ligase - by Bioz Stars, 2020-02
    93/100 stars
      Buy from Supplier

    Image Search Results