bst 2 0 dna polymerase  (New England Biolabs)


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    Name:
    Bst 2 0 DNA Polymerase
    Description:
    Bst 2 0 DNA Polymerase 8 000 units
    Catalog Number:
    m0537l
    Price:
    283
    Size:
    8 000 units
    Category:
    Thermostable DNA Polymerases
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    Structured Review

    New England Biolabs bst 2 0 dna polymerase
    Bst 2 0 DNA Polymerase
    Bst 2 0 DNA Polymerase 8 000 units
    https://www.bioz.com/result/bst 2 0 dna polymerase/product/New England Biolabs
    Average 99 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    bst 2 0 dna polymerase - by Bioz Stars, 2020-03
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Enhancement of Polymerase Activity of the Large Fragment in DNA Polymerase I from Geobacillus stearothermophilus by Site-Directed Mutagenesis at the Active Site
    Article Snippet: The optimal condition of IMSA assay was carried out in a 25 μ L volume containing the components: 12.5 μ L of 2x isothermal reaction mixture consisted of reaction buffer and dNTPs (Deaou Biotechnology, Guangzhou, China), 1.0 μ L of each primer (DsF and DsR, 5.0 mM; FIT and RIT, 20.0 mM; and SteF and SteR, 40.0 mM), 0.3 μ L of EV71 DNA template, and 1.0 μ L of commercial Bst 2.0 DNA polymerase (8 U/μ L; New England Biolabs, MA, USA) as positive control while the other tubes contained equal amounts of WT or mutant proteins, finally adding ddH2 O up to 25 μ L in reaction tubes. .. The first assay was the visual IMSA assay by adding 1.0 hydroxynaphthol blue (HNB) dye to the mixture before amplification, and the other assay was performed in a Deaou-308C constant temperature fluorescence detector (Deaou Biotechnology, Guangzhou, China) with the addition of 1.0 μ L diluted SYTO®9 fluorescent nucleic acid (Life Technologies, Gaithersburg, USA).

    Article Title: Ubiquitous L1 Mosaicism in Hippocampal Neurons
    Article Snippet: .. 5 μl Bst2.0 (NEB M0537L) enzyme mix was then added (10% Bst2.0 buffer, 10% Bst2.0) and reactions were placed on an Eppendorf ProCycler S thermal cycler and amplified using the following program: 4°C 10 sec, 1% ramp to 65°C 5 min, then 4 cycles of 94°C 10 sec, 4°C 10 sec, 1% ramp to 65°C 5 min, then 80°C 20 min with a 4°C hold. .. Individual Bst products were then evenly split into 3 individual PCR reactions.

    Article Title: Development of a single-tube one-step RT-LAMP assay to detect the Chikungunya virus genomeDevelopment and validation of four one-step real-time RT-LAMP assays for specific detection of each dengue virus serotype
    Article Snippet: The Genie II device allows to create an annealing curve for confirmation of amplification specificity by an additional heating and cooling step from 98°C to 80°C (0.05°C/s) for 6 min to allow the re-annealing of the amplified product. .. Bst 2.0 DNA Polymerase and Transcriptor Reverse Transcriptase were obtained from New England BioLabs (Hitchin, Herts, UK) and Roche, respectively.

    Article Title: Genome Filtering for New DNA Biomarkers of Loa loa Infection Suitable for Loop-Mediated Isothermal Amplification
    Article Snippet: LAMP Assays LAMP reactions contained 1.6 μM each of primers FIP and BIP, 0.2 μM each of F3 and B3, 0.4 μM each of LF and FB, 1.4 mM of each dNTP, 20 mM Tris-HCl (pH 8.8), 50 mM KCl, 10 mM (NH4 )2 SO4 , 8 mM MgSO4 , 0.1% Tween-20 and 8 U of Bst 2.0 DNA Polymerase (New England Biolabs) mixed with one of several template DNAs, or 10 mM Tris-HCl (pH 8), 0.1 mM EDTA for non-template controls (NTC), in a total volume of 25 μl. .. One μl of a 25X Valeramide (V) N,N-Diethylformamide (DEF) solution (final concentration of 34mM V/128 mM DEF) was added to some reactions to reduce non-specific amplification [ , ].

    Article Title: Genetic stability of genome-scale deoptimized RNA virus vaccine candidates under selective pressure
    Article Snippet: The DNA further underwent nick translation using Bst 2.0 DNA Polymerase and Buffer (NEB). .. Approximately 100 ng DNA libraries were then added into a PCR mix using the Platinum High Fidelity DNA Polymerase Master Mix (Life Technologies) followed by two cycles of PCR amplification at 95 °C for 10 min, two cycles at 95 °C for 30 s, two cycles at 58 °C for 30 s, and two cycles at 72 °C for 30 s The PCR products were further purified with 1.8 vol Agencourt, and DNA was recovered using a magnetic rack as described above.

    Article Title: Susceptibility of Exopalaemon carinicauda to the Infection with Shrimp Hemocyte Iridescent Virus (SHIV 20141215), a Strain of Decapod Iridescent Virus 1 (DIV1)
    Article Snippet: Paragraph title: 2.6. Loop-Mediated Isothermal Amplification (LAMP) ... Each LAMP mixture contained 1.6 µM each of inner primers FIP and BIP, 0.8 µM each of loop primers LF and LB, 0.2 µM each of outer primers F3 and B3, 1.4 mM of dNTP mix (TaKaRa, Dalian, China), 1.2 M betaine (Solarbio, Beijing, China), 25 µM calcein (Sigma, St. Louis, MO, USA), 500 µM MnCl2 , 6 mM MgCl2 , 8 U Bst 2.0 DNA polymerase (New England Biolabs Inc., Beverly, USA), 1× supplied buffer and the specified amount of template DNA in a final volume of 25 µL.

    Article Title: Diagnostic Accuracy of Loop-mediated Isothermal Amplification Assay as a Field Molecular Tool for Rapid Mass Screening of Old World Leishmania Infections in Sand Flies and In Vitro Culture
    Article Snippet: .. Theses sensitivity discrepancies among mentioned methods are described by some facts: first, activity of Taq DNA polymerase during PCR amplification is inhibited by tissue/blood components of sand flies such as myoglobin and protoporphyrin ( , – ) while, the Bst 2.0 DNA polymerase to overcome potential inhibitors in crude sand fly templates during LAMP amplification. .. Second, in a low Leishmania burden, a considerable amount of parasite DNA is lost during the extraction and purification processes that in this case, a set of PCR primers cannot be specifically annealed and amplified target templates ( ).

    Whole Genome Amplification:

    Article Title: Ubiquitous L1 Mosaicism in Hippocampal Neurons
    Article Snippet: Paragraph title: Single-Cell Whole-Genome Amplification ... 5 μl Bst2.0 (NEB M0537L) enzyme mix was then added (10% Bst2.0 buffer, 10% Bst2.0) and reactions were placed on an Eppendorf ProCycler S thermal cycler and amplified using the following program: 4°C 10 sec, 1% ramp to 65°C 5 min, then 4 cycles of 94°C 10 sec, 4°C 10 sec, 1% ramp to 65°C 5 min, then 80°C 20 min with a 4°C hold.

    Positive Control:

    Article Title: Enhancement of Polymerase Activity of the Large Fragment in DNA Polymerase I from Geobacillus stearothermophilus by Site-Directed Mutagenesis at the Active Site
    Article Snippet: .. The optimal condition of IMSA assay was carried out in a 25 μ L volume containing the components: 12.5 μ L of 2x isothermal reaction mixture consisted of reaction buffer and dNTPs (Deaou Biotechnology, Guangzhou, China), 1.0 μ L of each primer (DsF and DsR, 5.0 mM; FIT and RIT, 20.0 mM; and SteF and SteR, 40.0 mM), 0.3 μ L of EV71 DNA template, and 1.0 μ L of commercial Bst 2.0 DNA polymerase (8 U/μ L; New England Biolabs, MA, USA) as positive control while the other tubes contained equal amounts of WT or mutant proteins, finally adding ddH2 O up to 25 μ L in reaction tubes. .. The first assay was the visual IMSA assay by adding 1.0 hydroxynaphthol blue (HNB) dye to the mixture before amplification, and the other assay was performed in a Deaou-308C constant temperature fluorescence detector (Deaou Biotechnology, Guangzhou, China) with the addition of 1.0 μ L diluted SYTO®9 fluorescent nucleic acid (Life Technologies, Gaithersburg, USA).

    Synthesized:

    Article Title: Enhancement of Polymerase Activity of the Large Fragment in DNA Polymerase I from Geobacillus stearothermophilus by Site-Directed Mutagenesis at the Active Site
    Article Snippet: The primers for the IMSA assay was designed with the aid of Primer Explorer V4 [ ] shown in and synthesized by Huada Gene Co., Ltd. (Shenzhen, China). .. The optimal condition of IMSA assay was carried out in a 25 μ L volume containing the components: 12.5 μ L of 2x isothermal reaction mixture consisted of reaction buffer and dNTPs (Deaou Biotechnology, Guangzhou, China), 1.0 μ L of each primer (DsF and DsR, 5.0 mM; FIT and RIT, 20.0 mM; and SteF and SteR, 40.0 mM), 0.3 μ L of EV71 DNA template, and 1.0 μ L of commercial Bst 2.0 DNA polymerase (8 U/μ L; New England Biolabs, MA, USA) as positive control while the other tubes contained equal amounts of WT or mutant proteins, finally adding ddH2 O up to 25 μ L in reaction tubes.

    Incubation:

    Article Title: Development and validation of four one-step real-time RT-LAMP assays for specific detection of each dengue virus serotypeDevelopment of a single-tube one-step RT-LAMP assay to detect the Chikungunya virus genome
    Article Snippet: Each reaction consisted of 1x RM Trehalose, 6 mM MgSO4 , 5% polyethylene glycol (PEG), 1 μL fluorochrome dye (FD), 8 U Bst 2.0 DNA Polymerase (New England BioLabs, Hitchin, Herts, UK), 10 U Transcriptor Reverse Transcriptase (Roche) and 1 μL template (DENV RNA or H2 O as negative control). .. Before adding the Bst 2.0 DNA Polymerase, Transcriptor Reverse Transcriptase and template, mixes were incubated at 95°C for 5 min to melt any primer multi-mers and cooled immediately on ice for 5 min.

    Article Title: Development of a single-tube one-step RT-LAMP assay to detect the Chikungunya virus genomeDevelopment and validation of four one-step real-time RT-LAMP assays for specific detection of each dengue virus serotype
    Article Snippet: Before adding Bst 2.0 DNA Polymerase, Transcriptor Reverse Transcriptase and template, mixes were incubated at 95°C for 5 min to melt any primer multimers and cooled immediately on ice for 5 min. RM Trehalose, MgSO4 , PEG and FD were supplied by MAST Diagnostica GmbH (Reinfeld, Germany). .. Bst 2.0 DNA Polymerase and Transcriptor Reverse Transcriptase were obtained from New England BioLabs (Hitchin, Herts, UK) and Roche, respectively.

    Article Title: Genome Filtering for New DNA Biomarkers of Loa loa Infection Suitable for Loop-Mediated Isothermal Amplification
    Article Snippet: LAMP Assays LAMP reactions contained 1.6 μM each of primers FIP and BIP, 0.2 μM each of F3 and B3, 0.4 μM each of LF and FB, 1.4 mM of each dNTP, 20 mM Tris-HCl (pH 8.8), 50 mM KCl, 10 mM (NH4 )2 SO4 , 8 mM MgSO4 , 0.1% Tween-20 and 8 U of Bst 2.0 DNA Polymerase (New England Biolabs) mixed with one of several template DNAs, or 10 mM Tris-HCl (pH 8), 0.1 mM EDTA for non-template controls (NTC), in a total volume of 25 μl. .. Reactions were incubated at 61°C for 60–90 minutes in a Loopamp Realtime Turbidimeter (LA-320c, Eiken Chemical Co.).

    Activity Assay:

    Article Title: Enhancement of Polymerase Activity of the Large Fragment in DNA Polymerase I from Geobacillus stearothermophilus by Site-Directed Mutagenesis at the Active Site
    Article Snippet: Paragraph title: 2.5. Enzymatic Activity and Protein Assays ... The optimal condition of IMSA assay was carried out in a 25 μ L volume containing the components: 12.5 μ L of 2x isothermal reaction mixture consisted of reaction buffer and dNTPs (Deaou Biotechnology, Guangzhou, China), 1.0 μ L of each primer (DsF and DsR, 5.0 mM; FIT and RIT, 20.0 mM; and SteF and SteR, 40.0 mM), 0.3 μ L of EV71 DNA template, and 1.0 μ L of commercial Bst 2.0 DNA polymerase (8 U/μ L; New England Biolabs, MA, USA) as positive control while the other tubes contained equal amounts of WT or mutant proteins, finally adding ddH2 O up to 25 μ L in reaction tubes.

    Article Title: Genome Filtering for New DNA Biomarkers of Loa loa Infection Suitable for Loop-Mediated Isothermal Amplification
    Article Snippet: LAMP Assays LAMP reactions contained 1.6 μM each of primers FIP and BIP, 0.2 μM each of F3 and B3, 0.4 μM each of LF and FB, 1.4 mM of each dNTP, 20 mM Tris-HCl (pH 8.8), 50 mM KCl, 10 mM (NH4 )2 SO4 , 8 mM MgSO4 , 0.1% Tween-20 and 8 U of Bst 2.0 DNA Polymerase (New England Biolabs) mixed with one of several template DNAs, or 10 mM Tris-HCl (pH 8), 0.1 mM EDTA for non-template controls (NTC), in a total volume of 25 μl. .. The instrument measures the change in turbidity at 650 nm caused by the precipitation of magnesium pyrophosphate produced by polymerase activity.

    Article Title: Diagnostic Accuracy of Loop-mediated Isothermal Amplification Assay as a Field Molecular Tool for Rapid Mass Screening of Old World Leishmania Infections in Sand Flies and In Vitro Culture
    Article Snippet: .. Theses sensitivity discrepancies among mentioned methods are described by some facts: first, activity of Taq DNA polymerase during PCR amplification is inhibited by tissue/blood components of sand flies such as myoglobin and protoporphyrin ( , – ) while, the Bst 2.0 DNA polymerase to overcome potential inhibitors in crude sand fly templates during LAMP amplification. .. Second, in a low Leishmania burden, a considerable amount of parasite DNA is lost during the extraction and purification processes that in this case, a set of PCR primers cannot be specifically annealed and amplified target templates ( ).

    Flow Cytometry:

    Article Title: Direct Blood Dry LAMP: A Rapid, Stable, and Easy Diagnostic Tool for Human African Trypanosomiasis
    Article Snippet: .. 1.6 μl of 2M trehalose, 1.4 μl of dNTPs (25mM each), 0.05 μl of Bst 2.0 WS DNA polymerase (120 U/μl), 0.25 μl of Bst 2.0WS DNA polymerase (8 U/μl), 0.1 μl of RNase inhibitor (40 U/μl; Nakaraitesque), and 0.04 μl of AMV reverse transcriptase (20 U/μl; Nippongene) were mixed and air-dried for 15 min under a flow of clean air. ..

    Ligation:

    Article Title: Genetic stability of genome-scale deoptimized RNA virus vaccine candidates under selective pressure
    Article Snippet: The ligation reaction was carried out at room temperature for 30 min and terminated by adding 4 μL 0.5 M EDTA, pH 8.0. .. The DNA further underwent nick translation using Bst 2.0 DNA Polymerase and Buffer (NEB).

    Nick Translation:

    Article Title: Genetic stability of genome-scale deoptimized RNA virus vaccine candidates under selective pressure
    Article Snippet: .. The DNA further underwent nick translation using Bst 2.0 DNA Polymerase and Buffer (NEB). .. Digested DNA was purified using a spin column MinElute Kit (Qiagen).

    Infection:

    Article Title: Susceptibility of Exopalaemon carinicauda to the Infection with Shrimp Hemocyte Iridescent Virus (SHIV 20141215), a Strain of Decapod Iridescent Virus 1 (DIV1)
    Article Snippet: Each LAMP mixture contained 1.6 µM each of inner primers FIP and BIP, 0.8 µM each of loop primers LF and LB, 0.2 µM each of outer primers F3 and B3, 1.4 mM of dNTP mix (TaKaRa, Dalian, China), 1.2 M betaine (Solarbio, Beijing, China), 25 µM calcein (Sigma, St. Louis, MO, USA), 500 µM MnCl2 , 6 mM MgCl2 , 8 U Bst 2.0 DNA polymerase (New England Biolabs Inc., Beverly, USA), 1× supplied buffer and the specified amount of template DNA in a final volume of 25 µL. .. Detection specificity of the LAMP primers were examined using 100 ng of the total DNA extracted from uninfected prawns and shrimp infected with other pathogens, including WSSV, Vp AHPND , IHHNV, and EHP.

    RT Lamp Assay:

    Article Title: Development and validation of four one-step real-time RT-LAMP assays for specific detection of each dengue virus serotypeDevelopment of a single-tube one-step RT-LAMP assay to detect the Chikungunya virus genome
    Article Snippet: Each reaction consisted of 1x RM Trehalose, 6 mM MgSO4 , 5% polyethylene glycol (PEG), 1 μL fluorochrome dye (FD), 8 U Bst 2.0 DNA Polymerase (New England BioLabs, Hitchin, Herts, UK), 10 U Transcriptor Reverse Transcriptase (Roche) and 1 μL template (DENV RNA or H2 O as negative control). .. For each primer set per RT-LAMP assay, the final concentrations was as follows: 50 nM F3, 50 nM B3, 400 nM FIP, 400 nM BIP, 200 nM FLOOP, 200 nM BLOOP.

    Sequencing:

    Article Title: Genetic stability of genome-scale deoptimized RNA virus vaccine candidates under selective pressure
    Article Snippet: Paragraph title: Ion Torrent Deep Sequencing. ... The DNA further underwent nick translation using Bst 2.0 DNA Polymerase and Buffer (NEB).

    Article Title: Susceptibility of Exopalaemon carinicauda to the Infection with Shrimp Hemocyte Iridescent Virus (SHIV 20141215), a Strain of Decapod Iridescent Virus 1 (DIV1)
    Article Snippet: These primers compared with the database in NCBI ( http://blast.ncbi.nlm.nih.gov/Blast.cgi ) to analyze sequence similarities. .. Each LAMP mixture contained 1.6 µM each of inner primers FIP and BIP, 0.8 µM each of loop primers LF and LB, 0.2 µM each of outer primers F3 and B3, 1.4 mM of dNTP mix (TaKaRa, Dalian, China), 1.2 M betaine (Solarbio, Beijing, China), 25 µM calcein (Sigma, St. Louis, MO, USA), 500 µM MnCl2 , 6 mM MgCl2 , 8 U Bst 2.0 DNA polymerase (New England Biolabs Inc., Beverly, USA), 1× supplied buffer and the specified amount of template DNA in a final volume of 25 µL.

    Recombinant:

    Article Title: Promoter RNA sequencing (PRSeq) for the massive and quantitative promoter analysis in vitro
    Article Snippet: The reaction was performed with homemade recombinant Taq polymerase in 400-µL reaction volume by the following thermal conditions; 96 °C, 3 min; 50 °C, 2.5 min; 60 °C, 2.5 min; 72 °C, 5 min. .. Next, nicking and strand displacement elongation reactions in 200-µL volume were performed with Nt.Alw I (New England Biolabs, Ipswich, MA, USA) and Bst 2.0 DNA polymerase (New England Biolabs), respectively, according to the manufacture’s protocols.

    Fluorescence:

    Article Title: Enhancement of Polymerase Activity of the Large Fragment in DNA Polymerase I from Geobacillus stearothermophilus by Site-Directed Mutagenesis at the Active Site
    Article Snippet: The optimal condition of IMSA assay was carried out in a 25 μ L volume containing the components: 12.5 μ L of 2x isothermal reaction mixture consisted of reaction buffer and dNTPs (Deaou Biotechnology, Guangzhou, China), 1.0 μ L of each primer (DsF and DsR, 5.0 mM; FIT and RIT, 20.0 mM; and SteF and SteR, 40.0 mM), 0.3 μ L of EV71 DNA template, and 1.0 μ L of commercial Bst 2.0 DNA polymerase (8 U/μ L; New England Biolabs, MA, USA) as positive control while the other tubes contained equal amounts of WT or mutant proteins, finally adding ddH2 O up to 25 μ L in reaction tubes. .. The first assay was the visual IMSA assay by adding 1.0 hydroxynaphthol blue (HNB) dye to the mixture before amplification, and the other assay was performed in a Deaou-308C constant temperature fluorescence detector (Deaou Biotechnology, Guangzhou, China) with the addition of 1.0 μ L diluted SYTO®9 fluorescent nucleic acid (Life Technologies, Gaithersburg, USA).

    Article Title: Susceptibility of Exopalaemon carinicauda to the Infection with Shrimp Hemocyte Iridescent Virus (SHIV 20141215), a Strain of Decapod Iridescent Virus 1 (DIV1)
    Article Snippet: Each LAMP mixture contained 1.6 µM each of inner primers FIP and BIP, 0.8 µM each of loop primers LF and LB, 0.2 µM each of outer primers F3 and B3, 1.4 mM of dNTP mix (TaKaRa, Dalian, China), 1.2 M betaine (Solarbio, Beijing, China), 25 µM calcein (Sigma, St. Louis, MO, USA), 500 µM MnCl2 , 6 mM MgCl2 , 8 U Bst 2.0 DNA polymerase (New England Biolabs Inc., Beverly, USA), 1× supplied buffer and the specified amount of template DNA in a final volume of 25 µL. .. The procedure was 60 cycles for 60 °C, following 5 min at 85 °C on the CFX-96 Quantitative Fluorescence Instrument (BioRad, Hercules, CA, USA) using calcein fluorescent channel.

    Mutagenesis:

    Article Title: Enhancement of Polymerase Activity of the Large Fragment in DNA Polymerase I from Geobacillus stearothermophilus by Site-Directed Mutagenesis at the Active Site
    Article Snippet: .. The optimal condition of IMSA assay was carried out in a 25 μ L volume containing the components: 12.5 μ L of 2x isothermal reaction mixture consisted of reaction buffer and dNTPs (Deaou Biotechnology, Guangzhou, China), 1.0 μ L of each primer (DsF and DsR, 5.0 mM; FIT and RIT, 20.0 mM; and SteF and SteR, 40.0 mM), 0.3 μ L of EV71 DNA template, and 1.0 μ L of commercial Bst 2.0 DNA polymerase (8 U/μ L; New England Biolabs, MA, USA) as positive control while the other tubes contained equal amounts of WT or mutant proteins, finally adding ddH2 O up to 25 μ L in reaction tubes. .. The first assay was the visual IMSA assay by adding 1.0 hydroxynaphthol blue (HNB) dye to the mixture before amplification, and the other assay was performed in a Deaou-308C constant temperature fluorescence detector (Deaou Biotechnology, Guangzhou, China) with the addition of 1.0 μ L diluted SYTO®9 fluorescent nucleic acid (Life Technologies, Gaithersburg, USA).

    Size-exclusion Chromatography:

    Article Title: Ubiquitous L1 Mosaicism in Hippocampal Neurons
    Article Snippet: .. 5 μl Bst2.0 (NEB M0537L) enzyme mix was then added (10% Bst2.0 buffer, 10% Bst2.0) and reactions were placed on an Eppendorf ProCycler S thermal cycler and amplified using the following program: 4°C 10 sec, 1% ramp to 65°C 5 min, then 4 cycles of 94°C 10 sec, 4°C 10 sec, 1% ramp to 65°C 5 min, then 80°C 20 min with a 4°C hold. .. Individual Bst products were then evenly split into 3 individual PCR reactions.

    Purification:

    Article Title: Genetic stability of genome-scale deoptimized RNA virus vaccine candidates under selective pressure
    Article Snippet: Equal volumes of different ligated DNA libraries were then combined in a 1.5-mL LoBind Tube and purified with 1.8 vol Agencount, and the DNA libraries were recovered as described above. .. The DNA further underwent nick translation using Bst 2.0 DNA Polymerase and Buffer (NEB).

    Polymerase Chain Reaction:

    Article Title: Unusual isothermal multimerization and amplification by the strand-displacing DNA polymerases with reverse transcription activities
    Article Snippet: General information Bst DNA polymerase Large fragment (Bst LF), Bst 2.0 DNA polymerase (Bst 2.0), Bst 2.0 WarmStart DNA polymerase (Bst 2.0 WS), Bst 3.0 DNA polymerase (Bst 3.0), Klenow fragment polymerase (Klenow), Klenow fragment exo- polymerase (Klenow (exo-)), Vent exo- DNA polymerase (Vent (exo-)), and dNTP Mix were purchased from New England Biolabs. .. BcaBEST RNA PCR kit Ver.1.1, z-Taq, RTase M-MLV (RNase H-), Reverse Transcriptase XL (AMV) were purchased from Takara.

    Article Title: Ubiquitous L1 Mosaicism in Hippocampal Neurons
    Article Snippet: 5 μl Bst2.0 (NEB M0537L) enzyme mix was then added (10% Bst2.0 buffer, 10% Bst2.0) and reactions were placed on an Eppendorf ProCycler S thermal cycler and amplified using the following program: 4°C 10 sec, 1% ramp to 65°C 5 min, then 4 cycles of 94°C 10 sec, 4°C 10 sec, 1% ramp to 65°C 5 min, then 80°C 20 min with a 4°C hold. .. Individual Bst products were then evenly split into 3 individual PCR reactions.

    Article Title: A nuclease-polymerase chain reaction enables amplification of probes used for capture-based DNA target enrichment
    Article Snippet: .. BST–DSN reaction using PCR products as input BST 2.0 DNA polymerase (BST) and DSN were purchased from NEB and Sapphire North America, respectively. ..

    Article Title: Genetic stability of genome-scale deoptimized RNA virus vaccine candidates under selective pressure
    Article Snippet: The DNA further underwent nick translation using Bst 2.0 DNA Polymerase and Buffer (NEB). .. Approximately 100 ng DNA libraries were then added into a PCR mix using the Platinum High Fidelity DNA Polymerase Master Mix (Life Technologies) followed by two cycles of PCR amplification at 95 °C for 10 min, two cycles at 95 °C for 30 s, two cycles at 58 °C for 30 s, and two cycles at 72 °C for 30 s The PCR products were further purified with 1.8 vol Agencourt, and DNA was recovered using a magnetic rack as described above.

    Article Title: Diagnostic Accuracy of Loop-mediated Isothermal Amplification Assay as a Field Molecular Tool for Rapid Mass Screening of Old World Leishmania Infections in Sand Flies and In Vitro Culture
    Article Snippet: .. Theses sensitivity discrepancies among mentioned methods are described by some facts: first, activity of Taq DNA polymerase during PCR amplification is inhibited by tissue/blood components of sand flies such as myoglobin and protoporphyrin ( , – ) while, the Bst 2.0 DNA polymerase to overcome potential inhibitors in crude sand fly templates during LAMP amplification. .. Second, in a low Leishmania burden, a considerable amount of parasite DNA is lost during the extraction and purification processes that in this case, a set of PCR primers cannot be specifically annealed and amplified target templates ( ).

    IA:

    Article Title: Real-Time Sequence-Validated Loop-Mediated Isothermal Amplification Assays for Detection of Middle East Respiratory Syndrome Coronavirus (MERS-CoV)
    Article Snippet: All oligonucleotides and gBlocks were obtained from Integrated DNA Technologies (IDT, Corralville, IA, USA). .. All enzymes including Bst 2.0 DNA polymerase and AMV RT were obtained from New England Biolabs (NEB, Ipswich, MA, USA).

    Software:

    Article Title: A Simple Isothermal DNA Amplification Method to Screen Black Flies for Onchocerca volvulus Infection
    Article Snippet: LAMP assay LAMP reactions were performed in a final volume of 25 µL reaction buffer [10 mM Tris–HCl (pH 8.8), 50 mM KCl, 10 mM (NH4 )2 SO4 , 8 mM MgSO4 , and 0.1% Tween 20], 8 U Bst 2.0 DNA polymerase (New England Biolabs, Ipswich, MA, USA), (1.4 mM) of each deoxynucleoside triphosphate (dNTP), 1.6 mM of each FIP and BIP primer, 0.2 mM of each F3 and B3 primer, 0.4 mM of FLP and BLP, and 2 µL of target DNA. .. Turbidity data were analyzed using the LA-320c software package that reports when the change in turbidity over time (dT/dt) reaches a value of 0.1, which we then assigned to be the threshold time (Tt).

    Negative Control:

    Article Title: Development and validation of four one-step real-time RT-LAMP assays for specific detection of each dengue virus serotypeDevelopment of a single-tube one-step RT-LAMP assay to detect the Chikungunya virus genome
    Article Snippet: .. Each reaction consisted of 1x RM Trehalose, 6 mM MgSO4 , 5% polyethylene glycol (PEG), 1 μL fluorochrome dye (FD), 8 U Bst 2.0 DNA Polymerase (New England BioLabs, Hitchin, Herts, UK), 10 U Transcriptor Reverse Transcriptase (Roche) and 1 μL template (DENV RNA or H2 O as negative control). .. For each primer set per RT-LAMP assay, the final concentrations was as follows: 50 nM F3, 50 nM B3, 400 nM FIP, 400 nM BIP, 200 nM FLOOP, 200 nM BLOOP.

    Article Title: Development of a single-tube one-step RT-LAMP assay to detect the Chikungunya virus genomeDevelopment and validation of four one-step real-time RT-LAMP assays for specific detection of each dengue virus serotype
    Article Snippet: Each reaction consisted of 1x RM Trehalose, 6 mM MgSO4 , 5% polyethylene glycol (PEG), 1 μL fluorochrome dye (FD), 0.1 μM F3, 0.1 μM B3, 0.8 μM FIP, 0.8 μM BIP, 0.4 μM FLOOP, 0.4 μM BLOOP (final concentration for each set of primers), 8 U Bst 2.0 DNA Polymerase, 10 U Transcriptor Reverse Transcriptase and 1 μL template (RNA or H2 O as negative control). .. Bst 2.0 DNA Polymerase and Transcriptor Reverse Transcriptase were obtained from New England BioLabs (Hitchin, Herts, UK) and Roche, respectively.

    Ethanol Precipitation:

    Article Title: Promoter RNA sequencing (PRSeq) for the massive and quantitative promoter analysis in vitro
    Article Snippet: The DNA product was recovered by phenol/chloroform extraction and ethanol precipitation. .. Next, nicking and strand displacement elongation reactions in 200-µL volume were performed with Nt.Alw I (New England Biolabs, Ipswich, MA, USA) and Bst 2.0 DNA polymerase (New England Biolabs), respectively, according to the manufacture’s protocols.

    Multiple Annealing and Looping–Based Amplification Cycles:

    Article Title: Ubiquitous L1 Mosaicism in Hippocampal Neurons
    Article Snippet: Single-Cell Whole-Genome Amplification Whole genome amplification (WGA) was performed following extensive optimization of the MALBAC protocol ( ). .. 5 μl Bst2.0 (NEB M0537L) enzyme mix was then added (10% Bst2.0 buffer, 10% Bst2.0) and reactions were placed on an Eppendorf ProCycler S thermal cycler and amplified using the following program: 4°C 10 sec, 1% ramp to 65°C 5 min, then 4 cycles of 94°C 10 sec, 4°C 10 sec, 1% ramp to 65°C 5 min, then 80°C 20 min with a 4°C hold.

    Spectrophotometry:

    Article Title: Real-Time Sequence-Validated Loop-Mediated Isothermal Amplification Assays for Detection of Middle East Respiratory Syndrome Coronavirus (MERS-CoV)
    Article Snippet: The concentrations of the DNA and RNA suspensions were measured by UV spectrophotometry using the NanoDrop 1000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA). .. All enzymes including Bst 2.0 DNA polymerase and AMV RT were obtained from New England Biolabs (NEB, Ipswich, MA, USA).

    Produced:

    Article Title: Genome Filtering for New DNA Biomarkers of Loa loa Infection Suitable for Loop-Mediated Isothermal Amplification
    Article Snippet: LAMP Assays LAMP reactions contained 1.6 μM each of primers FIP and BIP, 0.2 μM each of F3 and B3, 0.4 μM each of LF and FB, 1.4 mM of each dNTP, 20 mM Tris-HCl (pH 8.8), 50 mM KCl, 10 mM (NH4 )2 SO4 , 8 mM MgSO4 , 0.1% Tween-20 and 8 U of Bst 2.0 DNA Polymerase (New England Biolabs) mixed with one of several template DNAs, or 10 mM Tris-HCl (pH 8), 0.1 mM EDTA for non-template controls (NTC), in a total volume of 25 μl. .. The instrument measures the change in turbidity at 650 nm caused by the precipitation of magnesium pyrophosphate produced by polymerase activity.

    Concentration Assay:

    Article Title: Development of a single-tube one-step RT-LAMP assay to detect the Chikungunya virus genomeDevelopment and validation of four one-step real-time RT-LAMP assays for specific detection of each dengue virus serotype
    Article Snippet: Each reaction consisted of 1x RM Trehalose, 6 mM MgSO4 , 5% polyethylene glycol (PEG), 1 μL fluorochrome dye (FD), 0.1 μM F3, 0.1 μM B3, 0.8 μM FIP, 0.8 μM BIP, 0.4 μM FLOOP, 0.4 μM BLOOP (final concentration for each set of primers), 8 U Bst 2.0 DNA Polymerase, 10 U Transcriptor Reverse Transcriptase and 1 μL template (RNA or H2 O as negative control). .. Bst 2.0 DNA Polymerase and Transcriptor Reverse Transcriptase were obtained from New England BioLabs (Hitchin, Herts, UK) and Roche, respectively.

    Article Title: Genome Filtering for New DNA Biomarkers of Loa loa Infection Suitable for Loop-Mediated Isothermal Amplification
    Article Snippet: LAMP Assays LAMP reactions contained 1.6 μM each of primers FIP and BIP, 0.2 μM each of F3 and B3, 0.4 μM each of LF and FB, 1.4 mM of each dNTP, 20 mM Tris-HCl (pH 8.8), 50 mM KCl, 10 mM (NH4 )2 SO4 , 8 mM MgSO4 , 0.1% Tween-20 and 8 U of Bst 2.0 DNA Polymerase (New England Biolabs) mixed with one of several template DNAs, or 10 mM Tris-HCl (pH 8), 0.1 mM EDTA for non-template controls (NTC), in a total volume of 25 μl. .. One μl of a 25X Valeramide (V) N,N-Diethylformamide (DEF) solution (final concentration of 34mM V/128 mM DEF) was added to some reactions to reduce non-specific amplification [ , ].

    Lamp Assay:

    Article Title: A Simple Isothermal DNA Amplification Method to Screen Black Flies for Onchocerca volvulus Infection
    Article Snippet: .. LAMP assay LAMP reactions were performed in a final volume of 25 µL reaction buffer [10 mM Tris–HCl (pH 8.8), 50 mM KCl, 10 mM (NH4 )2 SO4 , 8 mM MgSO4 , and 0.1% Tween 20], 8 U Bst 2.0 DNA polymerase (New England Biolabs, Ipswich, MA, USA), (1.4 mM) of each deoxynucleoside triphosphate (dNTP), 1.6 mM of each FIP and BIP primer, 0.2 mM of each F3 and B3 primer, 0.4 mM of FLP and BLP, and 2 µL of target DNA. .. Reactions were carried out using either a Loop Amp Realtime Turbidimeter (LA-320c, Eiken Chemical Co, Japan) or a 2720 Thermocycler (Applied Biosystems, USA) set at a constant temperature for colorimetric detection.

    Article Title: Diagnostic Accuracy of Loop-mediated Isothermal Amplification Assay as a Field Molecular Tool for Rapid Mass Screening of Old World Leishmania Infections in Sand Flies and In Vitro Culture
    Article Snippet: .. In this exploration, the occur-rence of non-specific figments due to laboratory contaminations and not to be affordable of Bst 2.0 DNA polymerase were addressed as the principal challenges of LAMP assay in our experiment. .. Conclusion The current LAMP technique was able to amplify the all Leishmania spp. in one tube reaction within the shortest time possible using heating block /normal water bath.

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    New England Biolabs bst 2 0 dna polymerase
    Bst 2 0 Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bst 2 0 dna polymerase/product/New England Biolabs
    Average 99 stars, based on 14 article reviews
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    bst 2 0 dna polymerase - by Bioz Stars, 2020-03
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