longamp hot start taq dna polymerase  (New England Biolabs)


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    Name:
    LongAmp Hot Start Taq DNA Polymerase
    Description:
    LongAmp Hot Start Taq DNA Polymerase 2 500 units
    Catalog Number:
    M0534L
    Price:
    441
    Category:
    Long distance PCR Kits
    Size:
    2 500 units
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    Structured Review

    New England Biolabs longamp hot start taq dna polymerase
    LongAmp Hot Start Taq DNA Polymerase
    LongAmp Hot Start Taq DNA Polymerase 2 500 units
    https://www.bioz.com/result/longamp hot start taq dna polymerase/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    longamp hot start taq dna polymerase - by Bioz Stars, 2021-07
    95/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Enhancement of homology-directed repair with chromatin donor templates in cells
    Article Snippet: While these possibilities may be unlikely, some PCR analysis of homozygous clones using more distant primers, again as performed by the Bradley laboratory (e.g. with LongAMP polymerase / NEB) would help ensure that these are bona fide targeted events. .. We performed long-range PCR on 115 of our apparently homozygous clones with LongAmp Hot Start Taq DNA Polymerase (NEB), as in Kosicki et al., (2018) (new Figure 2—figure supplement 2, Figure 2—figure supplement 4, and Figure 2—figure supplement 5; new Figure 3—figure supplement 2). .. We found that two out of the 115 apparently homozygous clones appeared to contain one chromosome with a properly edited allele and one chromosome with a large deletion in the other allele (new Figure 2—figure supplement 2).

    Article Title: Molecular Mechanism of Renal Tubule Transport: Expression of the B splice variant of NBCe1 (SLC4A4) in the mouse kidney
    Article Snippet: PCR amplification was performed using GoTaq Hot Start Polymerase (Promega, Madison, WI). .. When nested amplification was performed, the initial PCR amplification used LongAmp Hot Start Taq DNA Polymerase (New England Biolabs, Ipswich, MA). .. The PCR products were separated on agarose gels and bands were excised.

    Article Title: Enhancement of homology-directed repair with chromatin donor templates in cells
    Article Snippet: .. Then, genomic DNA from potentially homozygous GFP-positive clones was analyzed by long-range PCR analysis with LongAmp Hot Start Taq DNA Polymerase (NEB), as described by . ..

    Article Title: The Mitochondrial Genome of the Glomeromycete Rhizophagus sp. DAOM 213198 Reveals an Unusual Organization Consisting of Two Circular Chromosomes
    Article Snippet: Long-range PCR reactions were also performed to attempt to assemble the last two mt contigs. .. Long-range PCR reactions were done in a final volume of 50 µl using TaKaRa LA PCR Amplification Kit (Clontech, Mountain View, CA) according to the manufacturer’s instructions or using the PCR protocol for LongAmp Hot Start Taq DNA Polymerase (New England Biolabs). .. PCRs were run in an Eppendorf Mastercycler ProS (Eppendorf, Mississauga, ON).

    Clone Assay:

    Article Title: Enhancement of homology-directed repair with chromatin donor templates in cells
    Article Snippet: While these possibilities may be unlikely, some PCR analysis of homozygous clones using more distant primers, again as performed by the Bradley laboratory (e.g. with LongAMP polymerase / NEB) would help ensure that these are bona fide targeted events. .. We performed long-range PCR on 115 of our apparently homozygous clones with LongAmp Hot Start Taq DNA Polymerase (NEB), as in Kosicki et al., (2018) (new Figure 2—figure supplement 2, Figure 2—figure supplement 4, and Figure 2—figure supplement 5; new Figure 3—figure supplement 2). .. We found that two out of the 115 apparently homozygous clones appeared to contain one chromosome with a properly edited allele and one chromosome with a large deletion in the other allele (new Figure 2—figure supplement 2).

    Article Title: Enhancement of homology-directed repair with chromatin donor templates in cells
    Article Snippet: .. Then, genomic DNA from potentially homozygous GFP-positive clones was analyzed by long-range PCR analysis with LongAmp Hot Start Taq DNA Polymerase (NEB), as described by . ..

    Amplification:

    Article Title: Molecular Mechanism of Renal Tubule Transport: Expression of the B splice variant of NBCe1 (SLC4A4) in the mouse kidney
    Article Snippet: PCR amplification was performed using GoTaq Hot Start Polymerase (Promega, Madison, WI). .. When nested amplification was performed, the initial PCR amplification used LongAmp Hot Start Taq DNA Polymerase (New England Biolabs, Ipswich, MA). .. The PCR products were separated on agarose gels and bands were excised.

    Article Title: Detection of Cytomegalovirus Drug Resistance Mutations by Next-Generation Sequencing
    Article Snippet: .. The UL54 and UL97 genes were amplified using the LongAmp Hot Start Taq DNA polymerase (New England BioLabs, Ipswich, MA), according to the manufacturer's instructions. ..

    Article Title: The Mitochondrial Genome of the Glomeromycete Rhizophagus sp. DAOM 213198 Reveals an Unusual Organization Consisting of Two Circular Chromosomes
    Article Snippet: Long-range PCR reactions were also performed to attempt to assemble the last two mt contigs. .. Long-range PCR reactions were done in a final volume of 50 µl using TaKaRa LA PCR Amplification Kit (Clontech, Mountain View, CA) according to the manufacturer’s instructions or using the PCR protocol for LongAmp Hot Start Taq DNA Polymerase (New England Biolabs). .. PCRs were run in an Eppendorf Mastercycler ProS (Eppendorf, Mississauga, ON).

    Sequencing:

    Article Title: SMRT-Cappable-seq reveals complex operon variants in bacteria
    Article Snippet: For control RNA, the reaction products were purified using AMPure beads and eluted in 30 μl Low TE. .. SMRTbell library preparation and SMRT Sequencing ( )Second strand cDNA synthesis was performed on both control and enriched first strand cDNA/RNA duplex samples with LongAmp HotStart Taq DNA polymerase (New England Biolabs) using Pac_oligodC20 and Pac_rev primers following the manufacturer’s instructions. .. PCR cycle number optimization was used to determine the optimal amplification cycle number for the downstream large-scale PCR reactions as described in the standard iso-seq protocol ( http://www.pacb.com/wp-content/uploads/2015/09/User-Bulletin-Guidelines-for-Preparing-cDNA-Libraries-for-Isoform-Sequencing-Iso-Seq.pdf).

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  • 95
    New England Biolabs longamp hot start taq dna polymerase
    Longamp Hot Start Taq Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/longamp hot start taq dna polymerase/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    longamp hot start taq dna polymerase - by Bioz Stars, 2021-07
    95/100 stars
      Buy from Supplier

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