longamp hot start taq (New England Biolabs)

New England Biolabs is a verified supplier

New England Biolabs manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Name:

LongAmp Hot Start Taq DNA Polymerase

Description:

LongAmp Hot Start Taq DNA Polymerase 2 500 units

Catalog Number:

m0534l

Price:

441

Size:

2 500 units

Category:

Long distance PCR Kits

Buy from Supplier

Structured Review

New England Biolabs longamp hot start taq

LongAmp Hot Start Taq DNA Polymerase 2 500 units
https://www.bioz.com/result/longamp hot start taq/product/New England Biolabs
Average 93 stars, based on 16390 article reviews
Price from $9.99 to $1999.99

longamp hot start taq - by Bioz Stars, 2021-02

93/100 stars

Images

Clone Assay:

Article Title: Enhancement of homology-directed repair with chromatin donor templates in cells
Article Snippet: .. Then, genomic DNA from potentially homozygous GFP-positive clones was analyzed by long-range PCR analysis with LongAmp Hot Start Taq DNA Polymerase (NEB), as described by . ..

Amplification:

Article Title: EcoTILLING by sequencing reveals polymorphisms in genes encoding starch synthases that are associated with low glycemic response in rice
Article Snippet: .. PCR for amplification of EcoTILLING fragments High fidelity LongAmp® Taq DNA polymerase (Cat#M0534) obtained from New England Biolabs (NEB), Ipswich, UK was used for PCR amplification in a 50 μl reaction. ..

Concentration Assay:

Article Title: Comparative Analysis of the Shared Sex-Determination Region (SDR) among Salmonid Fishes
Article Snippet: .. For the first PCR, each reaction contained the following reagents: 1 µl EcoRI library, 1.25 µl forward (CKSDR_F12) and reverse (AP1) primers at 4 nM concentration, 3.25 µl nuclease free H2O, and 6.25 µl LongAmp Hot Start Taq 2 × Master Mix (NEB). ..

Polymerase Chain Reaction:

Article Title: Polymyxin Susceptibility in Pseudomonas aeruginosa Linked to the MexXY-OprM Multidrug Efflux System
Article Snippet: .. For colony PCR, the primers cbrA colony-F (5′-TCCTGGTCGTGGGCATCTAT-3′) and cbrA colony-R (5′-TTCTCCTGGCGGTCCTTGA-3′) and LongAmp Taq polymerase (New England BioLabs; 1 U) were used in a buffer containing 300 μM concentrations of each dNTP and 0.4 μM concentrations of each primer, with samples heated as described above for the Δ parR deletion except for an extension time of 3.5 min at 75°C. .. For the ΔPA2572 deletion, the upstream and downstream fragments were amplified using the primers PA2572Up-F (5′-CGAT GAATTC CGACGCGGGAGAAGTTCTTC-3′; the EcoRI site is underlined) and PA2572Up-R (5′-GATC GGATCC GTTCATGAGTGTCTGTCCACC-3′; the BamHI site is underlined) and the primers PA2572Down-F (5′-GATC GGATCC GAAGCGATCGAAGGCGCAC-3′; the BamHI site is underlined) and PA2572Down-R (5′-CGAT TCTAGA CGCTGCGCAAACTGGTCTC-3′; the XbaI site is underlined).

Article Title: A novel long intergenic noncoding RNA indispensable for the cleavage of mouse two‐cell embryos
Article Snippet: .. PCR was performed by LongAmpTM Taq DNA polymerase (NEB, #M0534L). ..

Sequencing:

Article Title: SMRT-Cappable-seq reveals complex operon variants in bacteria
Article Snippet: .. SMRTbell library preparation and SMRT Sequencing ( )Second strand cDNA synthesis was performed on both control and enriched first strand cDNA/RNA duplex samples with LongAmp HotStart Taq DNA polymerase (New England Biolabs) using Pac_oligodC20 and Pac_rev primers following the manufacturer’s instructions. .. PCR cycle number optimization was used to determine the optimal amplification cycle number for the downstream large-scale PCR reactions as described in the standard iso-seq protocol ( http://www.pacb.com/wp-content/uploads/2015/09/User-Bulletin-Guidelines-for-Preparing-cDNA-Libraries-for-Isoform-Sequencing-Iso-Seq.pdf).

Chloramphenicol Acetyltransferase Assay:

Article Title: EcoTILLING by sequencing reveals polymorphisms in genes encoding starch synthases that are associated with low glycemic response in rice
Article Snippet: .. PCR for amplification of EcoTILLING fragments High fidelity LongAmp® Taq DNA polymerase (CatM0534) obtained from New England Biolabs (NEB), Ipswich, UK was used for PCR amplification in a 50 μl reaction. ..

longamp hot start taq (New England Biolabs)

Structured Review

Related Products / Commonly Used Together