longamp hot start master mix  (New England Biolabs)


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    Name:
    LongAmp Hot Start Taq 2X Master Mix
    Description:
    LongAmp Hot Start Taq 2X Master Mix 500 rxns
    Catalog Number:
    M0533L
    Price:
    704
    Category:
    Long distance PCR Kits
    Size:
    500 rxns
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    Structured Review

    New England Biolabs longamp hot start master mix
    LongAmp Hot Start Taq 2X Master Mix
    LongAmp Hot Start Taq 2X Master Mix 500 rxns
    https://www.bioz.com/result/longamp hot start master mix/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    longamp hot start master mix - by Bioz Stars, 2021-06
    95/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: PCR-based analysis of mitochondrial DNA copy number, mitochondrial DNA damage, and nuclear DNA damage
    Article Snippet: Assuming a Poisson distribution of lesions (Ayala-Torres et al., 2000), the amplification for treated samples can be compared with the amplification for control samples and a relative lesion frequency can be calculated ( ; ). .. Nuclease-free H2 O LongAmp Hot Start Taq 2× Master Mix (New England Biolabs) 10 µM primers, diluted with 0.1× TE buffer Template DNA Sterile, aerosol filter tips and pipettes dedicated to LA-QPCR 0.2 ml PCR tubes 96-well format thermocycler Dedicated workstation (e.g. PCR hood equipped with UV lamp for sterilization) .. 1 UV-sterilize the work area.

    Article Title: Klebsiella oxytoca enterotoxins tilimycin and tilivalline have distinct host DNA-damaging and microtubule-stabilizing activities
    Article Snippet: In total, 3.5 ng of gDNA was used for Long Amplicon PCR ( , ). .. PCR was performed in 25-µL reactions containing 1× LongAmp Hot Start Taq2× Master Mix (New England Biolabs), 0.1 µM primer, and nuclease-free water. ..

    Article Title: Anchored pseudo-de novo assembly of human genomes identifies extensive sequence variation from unmapped sequence reads
    Article Snippet: The additional 18 samples (2/population, 6/ancestry) were previously unexamined and utilized to check predicted population frequencies. .. PCR amplification utilized 50 ng DNA, 1.25 μL (4 μM) forward and reverse primer, 6.25 μL LongAmp Hot Start 2× Master Mix (NEB) and H2 0 to total 13 μL. .. Thermocycler conditions were: 95 °C for 30 s, 30 cycles of [95 °C for 30 s, 56–60 °C for 30 s, 65 °C for 1–10 m], then 65 °C for 10 m. Products were run on 1.2 % agarose gels for 1 h and visually scored for presence.

    Article Title: Homotypic Dengue Virus Reinfections in Nicaraguan Children
    Article Snippet: Sequencing primers were designed using Primer3 software and an alignment of all whole-genome sequences from Nicaraguan DENV isolates available in GenBank (National Center for Biotechnology Information [NCBI]; 399 sequences). .. Reverse transcription was performed using SuperScript III Reverse Transcriptase (Life Technologies); long-range PCR was performed using the NEB LongAmp Hot Start Taq 2x Master Mix (New England Biolabs) according to the manufacturer's protocol. ..

    Article Title: Emu: Species-Level Microbial Community Profiling for Full-Length Nanopore 16S Reads
    Article Snippet: Synthetic gut mockLibrary construction and sequencing of V4 region of the 16S ribosomal RNA gene were performed using the NEXTflex 16S V4 Amplicon-Seq Kit 2.0 (Bioo Scientific, Austin, TX) with 20 ng of input DNA, and sequences were generated on the Illumina MiSeq platform (Illumina, San Diego, CA). .. Library construction and sequencing of the full-length 16S gene were performed with MinION nanopore sequencer (Oxford Nanopore Technologies, Oxford, UK) and 16S Barcoding Kit 1-24 (SQK-16S024, Oxford Nanopore Technologies, Oxford, UK).The PCR amplification and barcoding was completed with 15 ng of template DNA added to the LongAmp Hot Start Taq 2X Master Mix (New England Biolabs, Ipswich, MA). ..

    Article Title: HSB-1/HSF-1 pathway modulates histone H4 in mitochondria to control mtDNA transcription and longevity
    Article Snippet: The assay is based on the principle that DNA lesions block or inhibit the progression of DNA polymerases during PCR amplification of a large genomic fragment. .. Briefly, a 9.3-kb fragment of the unc-2 gene was amplified from template DNA of UV-damaged worms using LongAmp Hot Start Taq 2X Master Mix (New England Biolabs), and PCR primers were listed in table S5. .. The PCR product was quantified using the Quant-iT PicoGreen Double-Stranded DNA Assay Kit (Thermo Fisher Scientific).

    Hood:

    Article Title: PCR-based analysis of mitochondrial DNA copy number, mitochondrial DNA damage, and nuclear DNA damage
    Article Snippet: Assuming a Poisson distribution of lesions (Ayala-Torres et al., 2000), the amplification for treated samples can be compared with the amplification for control samples and a relative lesion frequency can be calculated ( ; ). .. Nuclease-free H2 O LongAmp Hot Start Taq 2× Master Mix (New England Biolabs) 10 µM primers, diluted with 0.1× TE buffer Template DNA Sterile, aerosol filter tips and pipettes dedicated to LA-QPCR 0.2 ml PCR tubes 96-well format thermocycler Dedicated workstation (e.g. PCR hood equipped with UV lamp for sterilization) .. 1 UV-sterilize the work area.

    Amplification:

    Article Title: Anchored pseudo-de novo assembly of human genomes identifies extensive sequence variation from unmapped sequence reads
    Article Snippet: The additional 18 samples (2/population, 6/ancestry) were previously unexamined and utilized to check predicted population frequencies. .. PCR amplification utilized 50 ng DNA, 1.25 μL (4 μM) forward and reverse primer, 6.25 μL LongAmp Hot Start 2× Master Mix (NEB) and H2 0 to total 13 μL. .. Thermocycler conditions were: 95 °C for 30 s, 30 cycles of [95 °C for 30 s, 56–60 °C for 30 s, 65 °C for 1–10 m], then 65 °C for 10 m. Products were run on 1.2 % agarose gels for 1 h and visually scored for presence.

    Article Title: Emu: Species-Level Microbial Community Profiling for Full-Length Nanopore 16S Reads
    Article Snippet: Synthetic gut mockLibrary construction and sequencing of V4 region of the 16S ribosomal RNA gene were performed using the NEXTflex 16S V4 Amplicon-Seq Kit 2.0 (Bioo Scientific, Austin, TX) with 20 ng of input DNA, and sequences were generated on the Illumina MiSeq platform (Illumina, San Diego, CA). .. Library construction and sequencing of the full-length 16S gene were performed with MinION nanopore sequencer (Oxford Nanopore Technologies, Oxford, UK) and 16S Barcoding Kit 1-24 (SQK-16S024, Oxford Nanopore Technologies, Oxford, UK).The PCR amplification and barcoding was completed with 15 ng of template DNA added to the LongAmp Hot Start Taq 2X Master Mix (New England Biolabs, Ipswich, MA). ..

    Article Title: HSB-1/HSF-1 pathway modulates histone H4 in mitochondria to control mtDNA transcription and longevity
    Article Snippet: The assay is based on the principle that DNA lesions block or inhibit the progression of DNA polymerases during PCR amplification of a large genomic fragment. .. Briefly, a 9.3-kb fragment of the unc-2 gene was amplified from template DNA of UV-damaged worms using LongAmp Hot Start Taq 2X Master Mix (New England Biolabs), and PCR primers were listed in table S5. .. The PCR product was quantified using the Quant-iT PicoGreen Double-Stranded DNA Assay Kit (Thermo Fisher Scientific).

    Sequencing:

    Article Title: Emu: Species-Level Microbial Community Profiling for Full-Length Nanopore 16S Reads
    Article Snippet: Synthetic gut mockLibrary construction and sequencing of V4 region of the 16S ribosomal RNA gene were performed using the NEXTflex 16S V4 Amplicon-Seq Kit 2.0 (Bioo Scientific, Austin, TX) with 20 ng of input DNA, and sequences were generated on the Illumina MiSeq platform (Illumina, San Diego, CA). .. Library construction and sequencing of the full-length 16S gene were performed with MinION nanopore sequencer (Oxford Nanopore Technologies, Oxford, UK) and 16S Barcoding Kit 1-24 (SQK-16S024, Oxford Nanopore Technologies, Oxford, UK).The PCR amplification and barcoding was completed with 15 ng of template DNA added to the LongAmp Hot Start Taq 2X Master Mix (New England Biolabs, Ipswich, MA). ..

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  • 95
    New England Biolabs longamp hot start master mix
    Longamp Hot Start Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/longamp hot start master mix/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    longamp hot start master mix - by Bioz Stars, 2021-06
    95/100 stars
      Buy from Supplier

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