q5u high fidelity dna polymerase  (New England Biolabs)


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    Name:
    Q5U Hot Start High Fidelity DNA Polymerase
    Description:
    Q5U Hot Start High Fidelity DNA Polymerase is a modified version of Q5 High Fidelity DNA Polymerase which efficiently incorporates dUTP and amplifies uracil containing DNA templates This feature is useful for amplifying bisulfite converted deaminated or damaged DNA preventing carryover contamination in PCR when used with dUTP and UDG and in USER cloning methods
    Catalog Number:
    M0515L
    Price:
    647
    Category:
    Thermostable DNA Polymerases
    Size:
    500 units
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    Structured Review

    New England Biolabs q5u high fidelity dna polymerase
    Q5U Hot Start High Fidelity DNA Polymerase
    Q5U Hot Start High Fidelity DNA Polymerase is a modified version of Q5 High Fidelity DNA Polymerase which efficiently incorporates dUTP and amplifies uracil containing DNA templates This feature is useful for amplifying bisulfite converted deaminated or damaged DNA preventing carryover contamination in PCR when used with dUTP and UDG and in USER cloning methods
    https://www.bioz.com/result/q5u high fidelity dna polymerase/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    q5u high fidelity dna polymerase - by Bioz Stars, 2021-04
    96/100 stars

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    Related Articles

    Amplification:

    Article Title: A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics
    Article Snippet: .. Addgene #98816) was amplified by PCR (M0515, Q5 Hot start, New England Biolabs) to introduce 5’ EcoRI and 3’ XbaI restriction enzyme sites flanking either ends of 3XNLS mScarlet-I sequence ( ; ). .. The entry vector pENTR1A-GFP-N2 (FR1) (a gift from Dr. Eric Campeau and Dr. Paul Kaufman, Addgene #19364) along with the purified PCR fragment was digested with EcoRI and XbaI then ligated together with T4 DNA ligase (M0202, New England Biolabs). pENTR1a-3XNLS-mScarlet-I was then recombined using Gateway LR Clonase II (11791, Invitrogen, Thermo Fisher Scientific) as per the manufacturer’s instructions into pLenti CMV Hygro DEST (W117-1, a gift from Dr. Eric Campeau and Dr. Paul Kaufman, Addgene #17454) to create the final vector which was sequence verified by Sanger sequencing (Australian Genome Research Facility).

    Article Title: EM-seq: Detection of DNA Methylation at Single Base Resolution from Picograms of DNA
    Article Snippet: .. DNA was deaminated using 0.26 μg of APOBEC3A in a 100 μl reaction volume for 3 h. 3 μl from 100 μl of enzymatically converted and 1 μl from 15 μl of bisulfite-converted DNA were PCR amplified with Q5U polymerase (NEB, Ipswich, MA) and primers (Supplemental Table 3) using the following cycling conditions: 98°C for 30 s, then 35 cycles of 98°C for 10 s, 63°C for 20 s, and 68°C for 2 min. ..

    Article Title: Preparation of E. coli RNA polymerase transcription elongation complexes for systematic RNA assays
    Article Snippet: All DNA templates that required translesion synthesis were assessed by both denaturing and non-denaturing PAGE quality control analyses, which are shown in . .. PCR Amplification DNA templates with dU bases were prepared using Q5U® High-Fidelity DNA Polymerase (NEB) in reactions that contained 1X Q5U® Buffer (NEB), 0.2 mM dNTPs (Invitrogen), 0.25 μM forward primer, 0.25 μM reverse primer, 20 pM template oligonucleotide, and 0.02 U/μl Q5U® . ..

    Article Title: DNA methylation dynamics during embryonic development and postnatal maturation of the mouse auditory organ of Corti
    Article Snippet: Each sample was spiked with 0.5% of total gDNA mass, Lambda unmethylated DNA to serve as an internal control for BS conversion rates. .. The initial prep was PCR amplified using KAPA HiFi Uracil+ polymerase with the index and universal Illumina compatible oligos, for 4-6 cycles. .. The library PCR was clean and size selected on AMPure XP beads.

    Polymerase Chain Reaction:

    Article Title: A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics
    Article Snippet: .. Addgene #98816) was amplified by PCR (M0515, Q5 Hot start, New England Biolabs) to introduce 5’ EcoRI and 3’ XbaI restriction enzyme sites flanking either ends of 3XNLS mScarlet-I sequence ( ; ). .. The entry vector pENTR1A-GFP-N2 (FR1) (a gift from Dr. Eric Campeau and Dr. Paul Kaufman, Addgene #19364) along with the purified PCR fragment was digested with EcoRI and XbaI then ligated together with T4 DNA ligase (M0202, New England Biolabs). pENTR1a-3XNLS-mScarlet-I was then recombined using Gateway LR Clonase II (11791, Invitrogen, Thermo Fisher Scientific) as per the manufacturer’s instructions into pLenti CMV Hygro DEST (W117-1, a gift from Dr. Eric Campeau and Dr. Paul Kaufman, Addgene #17454) to create the final vector which was sequence verified by Sanger sequencing (Australian Genome Research Facility).

    Article Title: EM-seq: Detection of DNA Methylation at Single Base Resolution from Picograms of DNA
    Article Snippet: .. DNA was deaminated using 0.26 μg of APOBEC3A in a 100 μl reaction volume for 3 h. 3 μl from 100 μl of enzymatically converted and 1 μl from 15 μl of bisulfite-converted DNA were PCR amplified with Q5U polymerase (NEB, Ipswich, MA) and primers (Supplemental Table 3) using the following cycling conditions: 98°C for 30 s, then 35 cycles of 98°C for 10 s, 63°C for 20 s, and 68°C for 2 min. ..

    Article Title: Preparation of E. coli RNA polymerase transcription elongation complexes for systematic RNA assays
    Article Snippet: All DNA templates that required translesion synthesis were assessed by both denaturing and non-denaturing PAGE quality control analyses, which are shown in . .. PCR Amplification DNA templates with dU bases were prepared using Q5U® High-Fidelity DNA Polymerase (NEB) in reactions that contained 1X Q5U® Buffer (NEB), 0.2 mM dNTPs (Invitrogen), 0.25 μM forward primer, 0.25 μM reverse primer, 20 pM template oligonucleotide, and 0.02 U/μl Q5U® . ..

    Article Title: DNA methylation dynamics during embryonic development and postnatal maturation of the mouse auditory organ of Corti
    Article Snippet: Each sample was spiked with 0.5% of total gDNA mass, Lambda unmethylated DNA to serve as an internal control for BS conversion rates. .. The initial prep was PCR amplified using KAPA HiFi Uracil+ polymerase with the index and universal Illumina compatible oligos, for 4-6 cycles. .. The library PCR was clean and size selected on AMPure XP beads.

    Introduce:

    Article Title: A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics
    Article Snippet: .. Addgene #98816) was amplified by PCR (M0515, Q5 Hot start, New England Biolabs) to introduce 5’ EcoRI and 3’ XbaI restriction enzyme sites flanking either ends of 3XNLS mScarlet-I sequence ( ; ). .. The entry vector pENTR1A-GFP-N2 (FR1) (a gift from Dr. Eric Campeau and Dr. Paul Kaufman, Addgene #19364) along with the purified PCR fragment was digested with EcoRI and XbaI then ligated together with T4 DNA ligase (M0202, New England Biolabs). pENTR1a-3XNLS-mScarlet-I was then recombined using Gateway LR Clonase II (11791, Invitrogen, Thermo Fisher Scientific) as per the manufacturer’s instructions into pLenti CMV Hygro DEST (W117-1, a gift from Dr. Eric Campeau and Dr. Paul Kaufman, Addgene #17454) to create the final vector which was sequence verified by Sanger sequencing (Australian Genome Research Facility).

    Sequencing:

    Article Title: A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics
    Article Snippet: .. Addgene #98816) was amplified by PCR (M0515, Q5 Hot start, New England Biolabs) to introduce 5’ EcoRI and 3’ XbaI restriction enzyme sites flanking either ends of 3XNLS mScarlet-I sequence ( ; ). .. The entry vector pENTR1A-GFP-N2 (FR1) (a gift from Dr. Eric Campeau and Dr. Paul Kaufman, Addgene #19364) along with the purified PCR fragment was digested with EcoRI and XbaI then ligated together with T4 DNA ligase (M0202, New England Biolabs). pENTR1a-3XNLS-mScarlet-I was then recombined using Gateway LR Clonase II (11791, Invitrogen, Thermo Fisher Scientific) as per the manufacturer’s instructions into pLenti CMV Hygro DEST (W117-1, a gift from Dr. Eric Campeau and Dr. Paul Kaufman, Addgene #17454) to create the final vector which was sequence verified by Sanger sequencing (Australian Genome Research Facility).

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  • 96
    New England Biolabs q5u high fidelity dna polymerase
    Q5u High Fidelity Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q5u high fidelity dna polymerase/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    q5u high fidelity dna polymerase - by Bioz Stars, 2021-04
    96/100 stars
      Buy from Supplier

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