β agarase  (New England Biolabs)


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  • 98
    Name:
    beta Agarase I
    Description:
    beta Agarase I 500 units
    Catalog Number:
    m0392l
    Price:
    322
    Size:
    500 units
    Category:
    Agarase
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    Structured Review

    New England Biolabs β agarase
    beta Agarase I
    beta Agarase I 500 units
    https://www.bioz.com/result/β agarase/product/New England Biolabs
    Average 98 stars, based on 94 article reviews
    Price from $9.99 to $1999.99
    β agarase - by Bioz Stars, 2020-07
    98/100 stars

    Images

    1) Product Images from "Molecular cloning, characterization and enzymatic properties of a novel ?eta-agarase from a marine isolate Psudoalteromonas SP. AG52"

    Article Title: Molecular cloning, characterization and enzymatic properties of a novel ?eta-agarase from a marine isolate Psudoalteromonas SP. AG52

    Journal: Brazilian Journal of Microbiology

    doi: 10.1590/S1517-83822010000400006

    Multiple alignment of β-agarase amino acid sequences of Pseudoalteromonas sp. AG52 with known agarases. The inverted triangles (▼) highlight the conserved catalytic residues, and black circles (●) represent the conserved residues involved in calcium ion binding. Identical residues in all sequences are shaded in gray and indicated by (*) under the column, conserved substitutions are indicated by (:), and semi-conserved substitutions are indicated by (.). Deletions are indicated by dashes. Sequence sources: Aeromonas sp. β-agarase (AAF03246), Pseudoalteromonas atlantica β-agarase I (AAA91888), Zobellia galactanivorans β-agarase A (AAF21820), Zobellia galactanivorans β-agarase B (AAF21821).
    Figure Legend Snippet: Multiple alignment of β-agarase amino acid sequences of Pseudoalteromonas sp. AG52 with known agarases. The inverted triangles (▼) highlight the conserved catalytic residues, and black circles (●) represent the conserved residues involved in calcium ion binding. Identical residues in all sequences are shaded in gray and indicated by (*) under the column, conserved substitutions are indicated by (:), and semi-conserved substitutions are indicated by (.). Deletions are indicated by dashes. Sequence sources: Aeromonas sp. β-agarase (AAF03246), Pseudoalteromonas atlantica β-agarase I (AAA91888), Zobellia galactanivorans β-agarase A (AAF21820), Zobellia galactanivorans β-agarase B (AAF21821).

    Techniques Used: Binding Assay, Sequencing

    Phylogenetic analysis of AgaA with known agarases based on amino acid sequence. Phylogenetic analysis was done by the Neighbor Joining method using MEGA3.1, based on sequence alignment using ClustalW (1.81). Numbers indicate the bootstrap confidence values of 1000 replicates. The accession numbers of the selected agarase sequences are as follows: AB178483, agarase ( Agarivorans sp. JAMB-A11); EF051475, QM38 agarase ( Agarivorans sp. QM38); EF100136, β-agarase ( Agarivorans sp. JA-1); AAA25696, β-agarase precursor ( Pseudoalteromonas atlantica ); AAP49346, AguB; AAP70390, AguH; AAP70365, AguK; AAP49316, AguD from uncultured bacterium; AAA91888, β-agarase I ( Pseudoalteromonas atlantica ); AAF03246, β-agarase ( Aeromonas sp.); AB124837, agarase ( Microbulbifer thermotolerans ); BAC99022, agarase ( Microbulbifer elongatus ); BAB79291, agarase, ( Pseudomonas sp. ND137; AAF21821, β-agarase B precursor ( Zobellia galactanivorans ); AAF21820, β-agarase A precursor ( Zobellia galactanivorans ); AAN39119, extracellular agarase precursor, ( Pseudoalteromonas sp. CY24); CAB61795, extracellular agarase precursor ( Streptomyces coelicolor A3); AAP70364, AguJ (uncultured bacterium); BAA04744, β-agarase ( Vibrio sp.); BAA03541, β-agarase ( Vibrio sp. JT0107); BAH16616, agarase ( Cellvibrio sp. OA-2007); AAT67062, β-agarase I ( Saccharophagus degradans ); AB160954, β-agarase ( Microbulbifer thermotolerans ).
    Figure Legend Snippet: Phylogenetic analysis of AgaA with known agarases based on amino acid sequence. Phylogenetic analysis was done by the Neighbor Joining method using MEGA3.1, based on sequence alignment using ClustalW (1.81). Numbers indicate the bootstrap confidence values of 1000 replicates. The accession numbers of the selected agarase sequences are as follows: AB178483, agarase ( Agarivorans sp. JAMB-A11); EF051475, QM38 agarase ( Agarivorans sp. QM38); EF100136, β-agarase ( Agarivorans sp. JA-1); AAA25696, β-agarase precursor ( Pseudoalteromonas atlantica ); AAP49346, AguB; AAP70390, AguH; AAP70365, AguK; AAP49316, AguD from uncultured bacterium; AAA91888, β-agarase I ( Pseudoalteromonas atlantica ); AAF03246, β-agarase ( Aeromonas sp.); AB124837, agarase ( Microbulbifer thermotolerans ); BAC99022, agarase ( Microbulbifer elongatus ); BAB79291, agarase, ( Pseudomonas sp. ND137; AAF21821, β-agarase B precursor ( Zobellia galactanivorans ); AAF21820, β-agarase A precursor ( Zobellia galactanivorans ); AAN39119, extracellular agarase precursor, ( Pseudoalteromonas sp. CY24); CAB61795, extracellular agarase precursor ( Streptomyces coelicolor A3); AAP70364, AguJ (uncultured bacterium); BAA04744, β-agarase ( Vibrio sp.); BAA03541, β-agarase ( Vibrio sp. JT0107); BAH16616, agarase ( Cellvibrio sp. OA-2007); AAT67062, β-agarase I ( Saccharophagus degradans ); AB160954, β-agarase ( Microbulbifer thermotolerans ).

    Techniques Used: Sequencing

    The nucleotide and deduced amino acid sequences of the β-agarase of Pseudoalteromonas sp. AG52. The predicted lipoprotein signal peptide is underlined and signal peptide sequence is in bold face. The start (ATG) and stop (TAA) codons are in bold italics and stop codon is marked with an asterisk (*). The GHF-16 β-agarase domain is in italics. Active sites and calcium binding residues are in boxes and dotted boxes, respectively.
    Figure Legend Snippet: The nucleotide and deduced amino acid sequences of the β-agarase of Pseudoalteromonas sp. AG52. The predicted lipoprotein signal peptide is underlined and signal peptide sequence is in bold face. The start (ATG) and stop (TAA) codons are in bold italics and stop codon is marked with an asterisk (*). The GHF-16 β-agarase domain is in italics. Active sites and calcium binding residues are in boxes and dotted boxes, respectively.

    Techniques Used: Sequencing, Binding Assay

    Related Articles

    Amplification:

    Article Title: Complex Chromosomal Rearrangements Mediated by Break-Induced Replication Involve Structure-Selective Endonucleases
    Article Snippet: .. Agarose plugs were incubated twice in 200 µl 1× β-Agarase I reaction buffer for 30 min, melted at 65°C for 10 min, equilibrated at 42°C for 15 min and treated with β-Agarase I (New England BioLabs) at 42°C for 1 h before PCR amplification. .. These were performed with 250 ng of genomic DNA (estimated with NanoDrop, Thermo Scientific) in a total volume of 30 µl in the following conditions: 1× Phusion HF buffer, 200 µM each dNTP, 0.6 U Phusion DNA polymerase (Finnzymes), 0.5 µM each primer.

    Agarose Gel Electrophoresis:

    Article Title: Tissue-Specific Chromatin Modifications at a Multigene Locus Generate Asymmetric Transcriptional Interactions
    Article Snippet: .. The resulting released BAC (named CD/hGH BAC ) insert was separated by pulsed-field gel electrophoresis (Bio-Rad, Hercules, CA) and purified from an excised slice of the agarose gel by digestion with 1 U/100 μl β-agarase I (New England Biolabs, Beverly, MA) followed by phenol-chloroform extraction. .. This purified 123-kb DNA fragment was used for microinjection as described above.

    Transgenic Assay:

    Article Title: Divergent Phenotypes in Mutant TDP-43 Transgenic Mice Highlight Potential Confounds in TDP-43 Transgenic Modeling
    Article Snippet: .. The transgenic fragment was obtained by BsrBI digestion, gel purified followed by β–agarase digestion (NEB), filtration and concentration. .. The modified TDP-43 transgene was injected into the pronuclei of donor FVB/NCr embryos (Charles River).

    Purification:

    Article Title: Cloning whole bacterial genomes in yeast.
    Article Snippet: .. Preparation of vector pARS-VN The vector pARS-VN (30) was linearized by a double digest with ClaI and XhoI, treated with antarctic phosphatase and gel purified using beta-agarase. .. A sample of 5 ng was used as template in a 100-ml PCR with Phusion DNA polymerase and HF buffer, according to the manufacturer’s protocol.

    Article Title: Tissue-Specific Chromatin Modifications at a Multigene Locus Generate Asymmetric Transcriptional Interactions
    Article Snippet: .. The resulting released BAC (named CD/hGH BAC ) insert was separated by pulsed-field gel electrophoresis (Bio-Rad, Hercules, CA) and purified from an excised slice of the agarose gel by digestion with 1 U/100 μl β-agarase I (New England Biolabs, Beverly, MA) followed by phenol-chloroform extraction. .. This purified 123-kb DNA fragment was used for microinjection as described above.

    Article Title: Divergent Phenotypes in Mutant TDP-43 Transgenic Mice Highlight Potential Confounds in TDP-43 Transgenic Modeling
    Article Snippet: .. The transgenic fragment was obtained by BsrBI digestion, gel purified followed by β–agarase digestion (NEB), filtration and concentration. .. The modified TDP-43 transgene was injected into the pronuclei of donor FVB/NCr embryos (Charles River).

    Article Title: Characterising the Canine Oral Microbiome by Direct Sequencing of Reverse-Transcribed rRNA Molecules
    Article Snippet: .. SSU rRNA was purified from agarose gels using β-Agarase I (New England Biolabs) following the manufacturer’s protocol with two modifications: 30 units of RNasin Plus Ribonuclease inhibitor (Promega) and 3 units of Turbo DNA-free (Ambion) were added. .. SSU rRNA was subsequently purified by precipitation with ¼ volume 10 M ammonium acetate and 2 x vol.

    Concentration Assay:

    Article Title: Divergent Phenotypes in Mutant TDP-43 Transgenic Mice Highlight Potential Confounds in TDP-43 Transgenic Modeling
    Article Snippet: .. The transgenic fragment was obtained by BsrBI digestion, gel purified followed by β–agarase digestion (NEB), filtration and concentration. .. The modified TDP-43 transgene was injected into the pronuclei of donor FVB/NCr embryos (Charles River).

    Incubation:

    Article Title: Complex Chromosomal Rearrangements Mediated by Break-Induced Replication Involve Structure-Selective Endonucleases
    Article Snippet: .. Agarose plugs were incubated twice in 200 µl 1× β-Agarase I reaction buffer for 30 min, melted at 65°C for 10 min, equilibrated at 42°C for 15 min and treated with β-Agarase I (New England BioLabs) at 42°C for 1 h before PCR amplification. .. These were performed with 250 ng of genomic DNA (estimated with NanoDrop, Thermo Scientific) in a total volume of 30 µl in the following conditions: 1× Phusion HF buffer, 200 µM each dNTP, 0.6 U Phusion DNA polymerase (Finnzymes), 0.5 µM each primer.

    Pulsed-Field Gel:

    Article Title: Tissue-Specific Chromatin Modifications at a Multigene Locus Generate Asymmetric Transcriptional Interactions
    Article Snippet: .. The resulting released BAC (named CD/hGH BAC ) insert was separated by pulsed-field gel electrophoresis (Bio-Rad, Hercules, CA) and purified from an excised slice of the agarose gel by digestion with 1 U/100 μl β-agarase I (New England Biolabs, Beverly, MA) followed by phenol-chloroform extraction. .. This purified 123-kb DNA fragment was used for microinjection as described above.

    BAC Assay:

    Article Title: Tissue-Specific Chromatin Modifications at a Multigene Locus Generate Asymmetric Transcriptional Interactions
    Article Snippet: .. The resulting released BAC (named CD/hGH BAC ) insert was separated by pulsed-field gel electrophoresis (Bio-Rad, Hercules, CA) and purified from an excised slice of the agarose gel by digestion with 1 U/100 μl β-agarase I (New England Biolabs, Beverly, MA) followed by phenol-chloroform extraction. .. This purified 123-kb DNA fragment was used for microinjection as described above.

    Polymerase Chain Reaction:

    Article Title: Complex Chromosomal Rearrangements Mediated by Break-Induced Replication Involve Structure-Selective Endonucleases
    Article Snippet: .. Agarose plugs were incubated twice in 200 µl 1× β-Agarase I reaction buffer for 30 min, melted at 65°C for 10 min, equilibrated at 42°C for 15 min and treated with β-Agarase I (New England BioLabs) at 42°C for 1 h before PCR amplification. .. These were performed with 250 ng of genomic DNA (estimated with NanoDrop, Thermo Scientific) in a total volume of 30 µl in the following conditions: 1× Phusion HF buffer, 200 µM each dNTP, 0.6 U Phusion DNA polymerase (Finnzymes), 0.5 µM each primer.

    Filtration:

    Article Title: Divergent Phenotypes in Mutant TDP-43 Transgenic Mice Highlight Potential Confounds in TDP-43 Transgenic Modeling
    Article Snippet: .. The transgenic fragment was obtained by BsrBI digestion, gel purified followed by β–agarase digestion (NEB), filtration and concentration. .. The modified TDP-43 transgene was injected into the pronuclei of donor FVB/NCr embryos (Charles River).

    Plasmid Preparation:

    Article Title: Cloning whole bacterial genomes in yeast.
    Article Snippet: .. Preparation of vector pARS-VN The vector pARS-VN (30) was linearized by a double digest with ClaI and XhoI, treated with antarctic phosphatase and gel purified using beta-agarase. .. A sample of 5 ng was used as template in a 100-ml PCR with Phusion DNA polymerase and HF buffer, according to the manufacturer’s protocol.

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    New England Biolabs β agarase
    Multiple alignment of <t>β-agarase</t> amino acid sequences of Pseudoalteromonas sp. AG52 with known agarases. The inverted triangles (▼) highlight the conserved catalytic residues, and black circles (●) represent the conserved residues involved in calcium ion binding. Identical residues in all sequences are shaded in gray and indicated by (*) under the column, conserved substitutions are indicated by (:), and semi-conserved substitutions are indicated by (.). Deletions are indicated by dashes. Sequence sources: Aeromonas sp. β-agarase (AAF03246), Pseudoalteromonas atlantica β-agarase I (AAA91888), Zobellia galactanivorans β-agarase A (AAF21820), Zobellia galactanivorans β-agarase B (AAF21821).
    β Agarase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β agarase/product/New England Biolabs
    Average 98 stars, based on 62 article reviews
    Price from $9.99 to $1999.99
    β agarase - by Bioz Stars, 2020-07
    98/100 stars
      Buy from Supplier

    Image Search Results


    Multiple alignment of β-agarase amino acid sequences of Pseudoalteromonas sp. AG52 with known agarases. The inverted triangles (▼) highlight the conserved catalytic residues, and black circles (●) represent the conserved residues involved in calcium ion binding. Identical residues in all sequences are shaded in gray and indicated by (*) under the column, conserved substitutions are indicated by (:), and semi-conserved substitutions are indicated by (.). Deletions are indicated by dashes. Sequence sources: Aeromonas sp. β-agarase (AAF03246), Pseudoalteromonas atlantica β-agarase I (AAA91888), Zobellia galactanivorans β-agarase A (AAF21820), Zobellia galactanivorans β-agarase B (AAF21821).

    Journal: Brazilian Journal of Microbiology

    Article Title: Molecular cloning, characterization and enzymatic properties of a novel ?eta-agarase from a marine isolate Psudoalteromonas SP. AG52

    doi: 10.1590/S1517-83822010000400006

    Figure Lengend Snippet: Multiple alignment of β-agarase amino acid sequences of Pseudoalteromonas sp. AG52 with known agarases. The inverted triangles (▼) highlight the conserved catalytic residues, and black circles (●) represent the conserved residues involved in calcium ion binding. Identical residues in all sequences are shaded in gray and indicated by (*) under the column, conserved substitutions are indicated by (:), and semi-conserved substitutions are indicated by (.). Deletions are indicated by dashes. Sequence sources: Aeromonas sp. β-agarase (AAF03246), Pseudoalteromonas atlantica β-agarase I (AAA91888), Zobellia galactanivorans β-agarase A (AAF21820), Zobellia galactanivorans β-agarase B (AAF21821).

    Article Snippet: Neoagarohexanitol (NA6) was purchased from Sigma (USA) and neoagarotetraose (NA4) and neoagarobiose (NA2) (NA4 + NA2) were prepared by digestion of neoagarohexanitol using commercial β-agarase (New England Biolab, USA).

    Techniques: Binding Assay, Sequencing

    Phylogenetic analysis of AgaA with known agarases based on amino acid sequence. Phylogenetic analysis was done by the Neighbor Joining method using MEGA3.1, based on sequence alignment using ClustalW (1.81). Numbers indicate the bootstrap confidence values of 1000 replicates. The accession numbers of the selected agarase sequences are as follows: AB178483, agarase ( Agarivorans sp. JAMB-A11); EF051475, QM38 agarase ( Agarivorans sp. QM38); EF100136, β-agarase ( Agarivorans sp. JA-1); AAA25696, β-agarase precursor ( Pseudoalteromonas atlantica ); AAP49346, AguB; AAP70390, AguH; AAP70365, AguK; AAP49316, AguD from uncultured bacterium; AAA91888, β-agarase I ( Pseudoalteromonas atlantica ); AAF03246, β-agarase ( Aeromonas sp.); AB124837, agarase ( Microbulbifer thermotolerans ); BAC99022, agarase ( Microbulbifer elongatus ); BAB79291, agarase, ( Pseudomonas sp. ND137; AAF21821, β-agarase B precursor ( Zobellia galactanivorans ); AAF21820, β-agarase A precursor ( Zobellia galactanivorans ); AAN39119, extracellular agarase precursor, ( Pseudoalteromonas sp. CY24); CAB61795, extracellular agarase precursor ( Streptomyces coelicolor A3); AAP70364, AguJ (uncultured bacterium); BAA04744, β-agarase ( Vibrio sp.); BAA03541, β-agarase ( Vibrio sp. JT0107); BAH16616, agarase ( Cellvibrio sp. OA-2007); AAT67062, β-agarase I ( Saccharophagus degradans ); AB160954, β-agarase ( Microbulbifer thermotolerans ).

    Journal: Brazilian Journal of Microbiology

    Article Title: Molecular cloning, characterization and enzymatic properties of a novel ?eta-agarase from a marine isolate Psudoalteromonas SP. AG52

    doi: 10.1590/S1517-83822010000400006

    Figure Lengend Snippet: Phylogenetic analysis of AgaA with known agarases based on amino acid sequence. Phylogenetic analysis was done by the Neighbor Joining method using MEGA3.1, based on sequence alignment using ClustalW (1.81). Numbers indicate the bootstrap confidence values of 1000 replicates. The accession numbers of the selected agarase sequences are as follows: AB178483, agarase ( Agarivorans sp. JAMB-A11); EF051475, QM38 agarase ( Agarivorans sp. QM38); EF100136, β-agarase ( Agarivorans sp. JA-1); AAA25696, β-agarase precursor ( Pseudoalteromonas atlantica ); AAP49346, AguB; AAP70390, AguH; AAP70365, AguK; AAP49316, AguD from uncultured bacterium; AAA91888, β-agarase I ( Pseudoalteromonas atlantica ); AAF03246, β-agarase ( Aeromonas sp.); AB124837, agarase ( Microbulbifer thermotolerans ); BAC99022, agarase ( Microbulbifer elongatus ); BAB79291, agarase, ( Pseudomonas sp. ND137; AAF21821, β-agarase B precursor ( Zobellia galactanivorans ); AAF21820, β-agarase A precursor ( Zobellia galactanivorans ); AAN39119, extracellular agarase precursor, ( Pseudoalteromonas sp. CY24); CAB61795, extracellular agarase precursor ( Streptomyces coelicolor A3); AAP70364, AguJ (uncultured bacterium); BAA04744, β-agarase ( Vibrio sp.); BAA03541, β-agarase ( Vibrio sp. JT0107); BAH16616, agarase ( Cellvibrio sp. OA-2007); AAT67062, β-agarase I ( Saccharophagus degradans ); AB160954, β-agarase ( Microbulbifer thermotolerans ).

    Article Snippet: Neoagarohexanitol (NA6) was purchased from Sigma (USA) and neoagarotetraose (NA4) and neoagarobiose (NA2) (NA4 + NA2) were prepared by digestion of neoagarohexanitol using commercial β-agarase (New England Biolab, USA).

    Techniques: Sequencing

    The nucleotide and deduced amino acid sequences of the β-agarase of Pseudoalteromonas sp. AG52. The predicted lipoprotein signal peptide is underlined and signal peptide sequence is in bold face. The start (ATG) and stop (TAA) codons are in bold italics and stop codon is marked with an asterisk (*). The GHF-16 β-agarase domain is in italics. Active sites and calcium binding residues are in boxes and dotted boxes, respectively.

    Journal: Brazilian Journal of Microbiology

    Article Title: Molecular cloning, characterization and enzymatic properties of a novel ?eta-agarase from a marine isolate Psudoalteromonas SP. AG52

    doi: 10.1590/S1517-83822010000400006

    Figure Lengend Snippet: The nucleotide and deduced amino acid sequences of the β-agarase of Pseudoalteromonas sp. AG52. The predicted lipoprotein signal peptide is underlined and signal peptide sequence is in bold face. The start (ATG) and stop (TAA) codons are in bold italics and stop codon is marked with an asterisk (*). The GHF-16 β-agarase domain is in italics. Active sites and calcium binding residues are in boxes and dotted boxes, respectively.

    Article Snippet: Neoagarohexanitol (NA6) was purchased from Sigma (USA) and neoagarotetraose (NA4) and neoagarobiose (NA2) (NA4 + NA2) were prepared by digestion of neoagarohexanitol using commercial β-agarase (New England Biolab, USA).

    Techniques: Sequencing, Binding Assay