l neb warmstart rtx reverse transcriptase  (New England Biolabs)


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    Name:
    WarmStart RTx Reverse Transcriptase
    Description:
    WarmStart RTx Reverse Transcriptase 250 rxns
    Catalog Number:
    m0380l
    Price:
    266
    Size:
    250 rxns
    Category:
    Reverse Transcriptases
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    New England Biolabs l neb warmstart rtx reverse transcriptase
    WarmStart RTx Reverse Transcriptase
    WarmStart RTx Reverse Transcriptase 250 rxns
    https://www.bioz.com/result/l neb warmstart rtx reverse transcriptase/product/New England Biolabs
    Average 96 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    l neb warmstart rtx reverse transcriptase - by Bioz Stars, 2021-02
    96/100 stars

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    1) Product Images from "A rapid, highly sensitive and open-access SARS-CoV-2 detection assay for laboratory and home testing"

    Article Title: A rapid, highly sensitive and open-access SARS-CoV-2 detection assay for laboratory and home testing

    Journal: bioRxiv

    doi: 10.1101/2020.06.23.166397

    A sensitive RT-LAMP assay based on open-access enzymes. A) RT-LAMP performance (measured as ‘time to threshold’) of different Bst DNA polymerase variants in combination with NEB’s RTx reverse transcriptase on synthetic SARS-CoV-2 RNA standard. For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND). Reactions were performed in duplicates; water was used as no-target control (NTC). B) RT-LAMP performance (measured as ‘time to threshold’) of different patent-protected (RTx, SuperScript III (SS-III)) and non-patent protected (AMV, HIV-1) reverse transcriptase enzymes in combination with Bst LF DNA polymerase on 500 copies/reaction of synthetic SARS-CoV-2 RNA standard. Reactions were performed in technical quadruplicates; water was used as no-target control (NTC). For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND). C) RT-LAMP sensitivity performance (measured as ‘time to threshold’) of reactions containing NEB RTx or home-made HIV-1 RT, in combination with Bst LF DNA polymerase. Reactions contained different amounts of synthetic SARS-CoV-2 RNA standard. Reactions were performed in technical duplicates; water was used as no-target control (NTC). For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND). D) Performance of RT-LAMP (measured as ‘time to threshold’) with different enzymatic compositions and QuickExtract patient sample as input. A pool of Covid-19 positive patient crude lysates (Pool N1-CDC, Cq 25) and a pool of Covid-19 negative crude lysates were tested in technical duplicates. For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND).
    Figure Legend Snippet: A sensitive RT-LAMP assay based on open-access enzymes. A) RT-LAMP performance (measured as ‘time to threshold’) of different Bst DNA polymerase variants in combination with NEB’s RTx reverse transcriptase on synthetic SARS-CoV-2 RNA standard. For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND). Reactions were performed in duplicates; water was used as no-target control (NTC). B) RT-LAMP performance (measured as ‘time to threshold’) of different patent-protected (RTx, SuperScript III (SS-III)) and non-patent protected (AMV, HIV-1) reverse transcriptase enzymes in combination with Bst LF DNA polymerase on 500 copies/reaction of synthetic SARS-CoV-2 RNA standard. Reactions were performed in technical quadruplicates; water was used as no-target control (NTC). For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND). C) RT-LAMP sensitivity performance (measured as ‘time to threshold’) of reactions containing NEB RTx or home-made HIV-1 RT, in combination with Bst LF DNA polymerase. Reactions contained different amounts of synthetic SARS-CoV-2 RNA standard. Reactions were performed in technical duplicates; water was used as no-target control (NTC). For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND). D) Performance of RT-LAMP (measured as ‘time to threshold’) with different enzymatic compositions and QuickExtract patient sample as input. A pool of Covid-19 positive patient crude lysates (Pool N1-CDC, Cq 25) and a pool of Covid-19 negative crude lysates were tested in technical duplicates. For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND).

    Techniques Used: RT Lamp Assay, Amplification

    2) Product Images from "A rapid, highly sensitive and open-access SARS-CoV-2 detection assay for laboratory and home testing"

    Article Title: A rapid, highly sensitive and open-access SARS-CoV-2 detection assay for laboratory and home testing

    Journal: bioRxiv

    doi: 10.1101/2020.06.23.166397

    A sensitive RT-LAMP assay based on open-access enzymes. A) RT-LAMP performance (measured as ‘time to threshold’) of different Bst DNA polymerase variants in combination with NEB’s RTx reverse transcriptase on synthetic SARS-CoV-2 RNA standard. For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND). Reactions were performed in duplicates; water was used as no-target control (NTC). B) RT-LAMP performance (measured as ‘time to threshold’) of different patent-protected (RTx, SuperScript III (SS-III)) and non-patent protected (AMV, HIV-1) reverse transcriptase enzymes in combination with Bst LF DNA polymerase on 500 copies/reaction of synthetic SARS-CoV-2 RNA standard. Reactions were performed in technical quadruplicates; water was used as no-target control (NTC). For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND). C) RT-LAMP sensitivity performance (measured as ‘time to threshold’) of reactions containing NEB RTx or home-made HIV-1 RT, in combination with Bst LF DNA polymerase. Reactions contained different amounts of synthetic SARS-CoV-2 RNA standard. Reactions were performed in technical duplicates; water was used as no-target control (NTC). For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND). D) Performance of RT-LAMP (measured as ‘time to threshold’) with different enzymatic compositions and QuickExtract patient sample as input. A pool of Covid-19 positive patient crude lysates (Pool N1-CDC, Cq 25) and a pool of Covid-19 negative crude lysates were tested in technical duplicates. For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND).
    Figure Legend Snippet: A sensitive RT-LAMP assay based on open-access enzymes. A) RT-LAMP performance (measured as ‘time to threshold’) of different Bst DNA polymerase variants in combination with NEB’s RTx reverse transcriptase on synthetic SARS-CoV-2 RNA standard. For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND). Reactions were performed in duplicates; water was used as no-target control (NTC). B) RT-LAMP performance (measured as ‘time to threshold’) of different patent-protected (RTx, SuperScript III (SS-III)) and non-patent protected (AMV, HIV-1) reverse transcriptase enzymes in combination with Bst LF DNA polymerase on 500 copies/reaction of synthetic SARS-CoV-2 RNA standard. Reactions were performed in technical quadruplicates; water was used as no-target control (NTC). For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND). C) RT-LAMP sensitivity performance (measured as ‘time to threshold’) of reactions containing NEB RTx or home-made HIV-1 RT, in combination with Bst LF DNA polymerase. Reactions contained different amounts of synthetic SARS-CoV-2 RNA standard. Reactions were performed in technical duplicates; water was used as no-target control (NTC). For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND). D) Performance of RT-LAMP (measured as ‘time to threshold’) with different enzymatic compositions and QuickExtract patient sample as input. A pool of Covid-19 positive patient crude lysates (Pool N1-CDC, Cq 25) and a pool of Covid-19 negative crude lysates were tested in technical duplicates. For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND).

    Techniques Used: RT Lamp Assay, Amplification

    Related Articles

    Amplification:

    Article Title: A rapid, highly sensitive and open-access SARS-CoV-2 detection assay for laboratory and home testing
    Article Snippet: .. dUTP/UDG contamination prevention system Reactions were set up to contain NEB 1x Isothermal Amplification Buffer, 1.4 mM of each dATP, dCTP, dGTP, 0.7 mM dUTP, 0.7 mM dTTP, 6 mM MgSO4 (100 mM stock, NEB), 0.32 U/µ l NEB Bst 2.0 polymerase, 0.3 U/µ l NEB Warmstart RTx Reverse Transcriptase, 0.2 U/µ l NEB Antarctic thermolabile UDG, sample and nuclease-free water. .. Reactions were set up on ice and incubated at room temperature for 5 minutes before being transferred to 63°C to start RT-LAMP reactions under standard conditions described above.

    Article Title: A rapid, highly sensitive and open-access SARS-CoV-2 detection assay for laboratory and home testing
    Article Snippet: .. For LAMP reactions using individual polymerases, RT-LAMP reactions were set up using NEB 1x Isothermal Amplification Buffer (Bst LF, Bst 2.0, Bst 3.0) or NEB 1x Isothermal Amplification Buffer II (Bst 3.0), 6 mM MgSO4 (8 mM final; 2 mM MgSO4 are present in Isothermal Buffer I), 0.3 U/µ l NEB Warmstart RTx, 0.32 U/µ l NEB Bst DNA polymerase (LF, 2.0 or 3.0), 1.4 mM of each dNTP (Larova, 25 mM of each dNTP stock solution), 1x fluorescent dye or 1 µM Syto9, sample and nuclease-free water. .. For LAMP reactions testing individual RT-enzymes, RT-LAMP reactions were set up using NEB 1x Isothermal Amplification Buffer (Bst LF, Bst 2.0, Bst 3.0) or NEB 1x Isothermal Amplification Buffer II (Bst 3.0), 6 mM MgSO4 (8 mM final; 2 mM MgSO4 are present in Isothermal Buffer I), 1.4 mM of each dNTP (Larova, 25 mM of each dNTP stock solution), 0.32 U/µ l NEB Bst DNA polymerase (LF, 2.0 or 3.0), 0.3 U/µ l, Warmstart RTx (NEB), 0.2 U/µ l AMV RT (NEB), 4 U/µ l SuperScript III (Thermofisher), 50 nM of home-made HIV-1 RT (BH10) diluted in 1x dilution buffer (TrisHCl pH 7.5, 50 mM NaCl, 0.5 mM TCEP) and 1x fluorescent dye or 1 µM Syto9, sample and nuclease-free water.

    Article Title: Ultrasensitive loop mediated isothermal amplification (US-LAMP) to detect malaria for elimination
    Article Snippet: .. Ultrasensitive loop mediated amplification (US-LAMP) assay conditions Bst 2.0 WarmStart® DNA polymerase was combined with WarmStart® reverse transcriptase in 1X Isothermal Amplification Buffer (New England Biolabs, Whitby, ON) to perform the US-LAMP assay. .. In a 25-µL LAMP reaction mixture, 1.6 µM F1P and B1P, 0.8 µM LPF and LPB, 0.2 µM F3 and B3 primer concentrations, 8 mM MgSO4 , 1.4 mM dNTPs, 0.8 M Betaine (Sigma-Aldrich, Oakville, ON, Canada), 8 unit of Bst 2.0 WarmStart® DNA Polymerase and 7.5 unit of Warm Start® reverse transcriptase were used.

    Recombinant:

    Article Title: Point of sampling detection of Zika virus within a multiplexed kit capable of detecting dengue and chikungunya
    Article Snippet: .. RT-LAMP procedure Reaction mixtures (50 μL total volume) contained a 10X primer set (5 μL, 16 μM FIP and BIP, 2 μM F3 and B3, 5 μM LF (or LB for chikungunya), 2 μM LB (or LF for chikungunya), 4 μM LF quencher probe, and 3 μM LB-fluorescent probe (or LF probe for chikungunya)), deoxynucleoside triphosphates (dNTPs, 1.4 mM of each), Tris-HCl buffer (20 mM, pH 8.8), KCl (50 mM), (NH4 )2 SO4 (10 mM,) MgSO4 (8 mM), Tween® 20 (0.1%), DTT (1 mM), Bst 2.0 WarmStart® DNA Polymerase (16 U, NEB, Ipswich, MA), WarmStart® RTx Reverse Transcriptase (15 U, NEB, Ipswich, MA), and RNaseOUT™ recombinant ribonuclease inhibitor (80 U, Thermo Fisher Scientific, Waltham, MA). ..

    Lamp Assay:

    Article Title: Optimization and clinical validation of dual-target RT-LAMP for SARS-CoV-2
    Article Snippet: .. 2.5 LAMP assay conditions The dual-target LAMP reaction was conducted using a combination of Warmstart Rtx Reverse Transcriptase (New England Biolab, Whitby, ON) with Bst 2.0 Warmstart DNA Polymerase (New England Biolab, Whitby, ON). .. In a 25 µL LAMP reaction mixture, 1.6 µM F1P and B1P, 0.8 µM LPF and LPB, 0.2 µM F3 and B3 primer concentrations, 8 mM MgSO4 , 1.4 mM dNTPs, 8 units of Bst 2.0 WarmStart® DNA Polymerase and 15 units of Warm Start® reverse transcriptase were used.

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  • 96
    New England Biolabs l neb warmstart rtx reverse transcriptase
    A sensitive RT-LAMP assay based on open-access enzymes. A) RT-LAMP performance (measured as ‘time to threshold’) of different Bst DNA polymerase variants in combination with <t>NEB’s</t> <t>RTx</t> reverse transcriptase on synthetic SARS-CoV-2 RNA standard. For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND). Reactions were performed in duplicates; water was used as no-target control (NTC). B) RT-LAMP performance (measured as ‘time to threshold’) of different patent-protected (RTx, SuperScript III (SS-III)) and non-patent protected (AMV, HIV-1) reverse transcriptase enzymes in combination with Bst LF DNA polymerase on 500 copies/reaction of synthetic SARS-CoV-2 RNA standard. Reactions were performed in technical quadruplicates; water was used as no-target control (NTC). For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND). C) RT-LAMP sensitivity performance (measured as ‘time to threshold’) of reactions containing NEB RTx or home-made HIV-1 RT, in combination with Bst LF DNA polymerase. Reactions contained different amounts of synthetic SARS-CoV-2 RNA standard. Reactions were performed in technical duplicates; water was used as no-target control (NTC). For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND). D) Performance of RT-LAMP (measured as ‘time to threshold’) with different enzymatic compositions and QuickExtract patient sample as input. A pool of Covid-19 positive patient crude lysates (Pool N1-CDC, Cq 25) and a pool of Covid-19 negative crude lysates were tested in technical duplicates. For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND).
    L Neb Warmstart Rtx Reverse Transcriptase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l neb warmstart rtx reverse transcriptase/product/New England Biolabs
    Average 96 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    l neb warmstart rtx reverse transcriptase - by Bioz Stars, 2021-02
    96/100 stars
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    A sensitive RT-LAMP assay based on open-access enzymes. A) RT-LAMP performance (measured as ‘time to threshold’) of different Bst DNA polymerase variants in combination with NEB’s RTx reverse transcriptase on synthetic SARS-CoV-2 RNA standard. For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND). Reactions were performed in duplicates; water was used as no-target control (NTC). B) RT-LAMP performance (measured as ‘time to threshold’) of different patent-protected (RTx, SuperScript III (SS-III)) and non-patent protected (AMV, HIV-1) reverse transcriptase enzymes in combination with Bst LF DNA polymerase on 500 copies/reaction of synthetic SARS-CoV-2 RNA standard. Reactions were performed in technical quadruplicates; water was used as no-target control (NTC). For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND). C) RT-LAMP sensitivity performance (measured as ‘time to threshold’) of reactions containing NEB RTx or home-made HIV-1 RT, in combination with Bst LF DNA polymerase. Reactions contained different amounts of synthetic SARS-CoV-2 RNA standard. Reactions were performed in technical duplicates; water was used as no-target control (NTC). For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND). D) Performance of RT-LAMP (measured as ‘time to threshold’) with different enzymatic compositions and QuickExtract patient sample as input. A pool of Covid-19 positive patient crude lysates (Pool N1-CDC, Cq 25) and a pool of Covid-19 negative crude lysates were tested in technical duplicates. For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND).

    Journal: bioRxiv

    Article Title: A rapid, highly sensitive and open-access SARS-CoV-2 detection assay for laboratory and home testing

    doi: 10.1101/2020.06.23.166397

    Figure Lengend Snippet: A sensitive RT-LAMP assay based on open-access enzymes. A) RT-LAMP performance (measured as ‘time to threshold’) of different Bst DNA polymerase variants in combination with NEB’s RTx reverse transcriptase on synthetic SARS-CoV-2 RNA standard. For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND). Reactions were performed in duplicates; water was used as no-target control (NTC). B) RT-LAMP performance (measured as ‘time to threshold’) of different patent-protected (RTx, SuperScript III (SS-III)) and non-patent protected (AMV, HIV-1) reverse transcriptase enzymes in combination with Bst LF DNA polymerase on 500 copies/reaction of synthetic SARS-CoV-2 RNA standard. Reactions were performed in technical quadruplicates; water was used as no-target control (NTC). For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND). C) RT-LAMP sensitivity performance (measured as ‘time to threshold’) of reactions containing NEB RTx or home-made HIV-1 RT, in combination with Bst LF DNA polymerase. Reactions contained different amounts of synthetic SARS-CoV-2 RNA standard. Reactions were performed in technical duplicates; water was used as no-target control (NTC). For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND). D) Performance of RT-LAMP (measured as ‘time to threshold’) with different enzymatic compositions and QuickExtract patient sample as input. A pool of Covid-19 positive patient crude lysates (Pool N1-CDC, Cq 25) and a pool of Covid-19 negative crude lysates were tested in technical duplicates. For reactions in which no amplification was recorded, ‘time to threshold’ is reported as ‘not detected’ (ND).

    Article Snippet: dUTP/UDG contamination prevention system Reactions were set up to contain NEB 1x Isothermal Amplification Buffer, 1.4 mM of each dATP, dCTP, dGTP, 0.7 mM dUTP, 0.7 mM dTTP, 6 mM MgSO4 (100 mM stock, NEB), 0.32 U/µ l NEB Bst 2.0 polymerase, 0.3 U/µ l NEB Warmstart RTx Reverse Transcriptase, 0.2 U/µ l NEB Antarctic thermolabile UDG, sample and nuclease-free water.

    Techniques: RT Lamp Assay, Amplification