magnesium sulfate mgso4 solution  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    WarmStart RTx Reverse Transcriptase
    Description:
    WarmStart RTx Reverse Transcriptase 250 rxns
    Catalog Number:
    m0380l
    Price:
    266
    Size:
    250 rxns
    Category:
    Reverse Transcriptases
    Buy from Supplier


    Structured Review

    New England Biolabs magnesium sulfate mgso4 solution
    WarmStart RTx Reverse Transcriptase
    WarmStart RTx Reverse Transcriptase 250 rxns
    https://www.bioz.com/result/magnesium sulfate mgso4 solution/product/New England Biolabs
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    magnesium sulfate mgso4 solution - by Bioz Stars, 2020-08
    99/100 stars

    Images

    Related Articles

    Recombinant:

    Article Title: Point of sampling detection of Zika virus within a multiplexed kit capable of detecting dengue and chikungunya
    Article Snippet: .. RT-LAMP procedure Reaction mixtures (50 μL total volume) contained a 10X primer set (5 μL, 16 μM FIP and BIP, 2 μM F3 and B3, 5 μM LF (or LB for chikungunya), 2 μM LB (or LF for chikungunya), 4 μM LF quencher probe, and 3 μM LB-fluorescent probe (or LF probe for chikungunya)), deoxynucleoside triphosphates (dNTPs, 1.4 mM of each), Tris-HCl buffer (20 mM, pH 8.8), KCl (50 mM), (NH4 )2 SO4 (10 mM,) MgSO4 (8 mM), Tween® 20 (0.1%), DTT (1 mM), Bst 2.0 WarmStart® DNA Polymerase (16 U, NEB, Ipswich, MA), WarmStart® RTx Reverse Transcriptase (15 U, NEB, Ipswich, MA), and RNaseOUT™ recombinant ribonuclease inhibitor (80 U, Thermo Fisher Scientific, Waltham, MA). ..

    Amplification:

    Article Title: Point-of-care testing for COVID-19 using SHERLOCK diagnostics
    Article Snippet: .. For Step (2) - STOPCovid detection reaction: - Bst 2.0 WarmStart® DNA Polymerase (M0538L), New England BioLabs - WarmStart® RTx Reverse Transcriptase (M0380L), New England BioLabs - 10X Isothermal Amplification Buffer (B0374S ), New England BioLabs, supplied with M0538L and M0380L - 100 mM MgSO 4 ( B1003S ), New England BioLabs, supplied with M0538L and M0380L - 10 mM Deoxynucleotide (dNTP) Solution Mix (N0447L ), New England BioLabs - Taurine (86329-100G ), Millipore Sigma - AapCas12b protein purified according to Kellner et al., Nature Protocols 2019, stored as 10 μL aliquots at 2 mg/mL. .. - Guide RNA for detecting N gene can be ordered from Synthego - Reporter DNA for lateral flow read out (Lateral Flow Reporter: /56-FAM/TTTTTTT/3Bio/), can be ordered from IDT - (Optional) Proteinase K Inhibitor (539470-10MG ), Millipore Sigma.

    Article Title: Ultrasensitive loop mediated isothermal amplification (US-LAMP) to detect malaria for elimination
    Article Snippet: .. Ultrasensitive loop mediated amplification (US-LAMP) assay conditions Bst 2.0 WarmStart® DNA polymerase was combined with WarmStart® reverse transcriptase in 1X Isothermal Amplification Buffer (New England Biolabs, Whitby, ON) to perform the US-LAMP assay. .. In a 25-µL LAMP reaction mixture, 1.6 µM F1P and B1P, 0.8 µM LPF and LPB, 0.2 µM F3 and B3 primer concentrations, 8 mM MgSO4 , 1.4 mM dNTPs, 0.8 M Betaine (Sigma-Aldrich, Oakville, ON, Canada), 8 unit of Bst 2.0 WarmStart® DNA Polymerase and 7.5 unit of Warm Start® reverse transcriptase were used.

    Purification:

    Article Title: Point-of-care testing for COVID-19 using SHERLOCK diagnostics
    Article Snippet: .. For Step (2) - STOPCovid detection reaction: - Bst 2.0 WarmStart® DNA Polymerase (M0538L), New England BioLabs - WarmStart® RTx Reverse Transcriptase (M0380L), New England BioLabs - 10X Isothermal Amplification Buffer (B0374S ), New England BioLabs, supplied with M0538L and M0380L - 100 mM MgSO 4 ( B1003S ), New England BioLabs, supplied with M0538L and M0380L - 10 mM Deoxynucleotide (dNTP) Solution Mix (N0447L ), New England BioLabs - Taurine (86329-100G ), Millipore Sigma - AapCas12b protein purified according to Kellner et al., Nature Protocols 2019, stored as 10 μL aliquots at 2 mg/mL. .. - Guide RNA for detecting N gene can be ordered from Synthego - Reporter DNA for lateral flow read out (Lateral Flow Reporter: /56-FAM/TTTTTTT/3Bio/), can be ordered from IDT - (Optional) Proteinase K Inhibitor (539470-10MG ), Millipore Sigma.

    Article Title: iSCAN: An RT-LAMP-coupled CRISPR-Cas12 module for rapid, sensitive detection of SARS-CoV-2
    Article Snippet: .. RTx reverse transcriptase was purified following Bhadra et. al 2020 protocol [43]. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    New England Biolabs magnesium sulfate mgso4 solution
    Magnesium Sulfate Mgso4 Solution, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/magnesium sulfate mgso4 solution/product/New England Biolabs
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    magnesium sulfate mgso4 solution - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results