exonuclease vii  (New England Biolabs)


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  • 97
    Name:
    Exonuclease VII
    Description:
    Exonuclease VII 1 000 units
    Catalog Number:
    m0379l
    Price:
    640
    Size:
    1 000 units
    Category:
    Exonucleases
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    Structured Review

    New England Biolabs exonuclease vii
    Exonuclease VII
    Exonuclease VII 1 000 units
    https://www.bioz.com/result/exonuclease vii/product/New England Biolabs
    Average 97 stars, based on 3395 article reviews
    Price from $9.99 to $1999.99
    exonuclease vii - by Bioz Stars, 2020-09
    97/100 stars

    Images

    1) Product Images from "ParB spreading on DNA requires cytidine triphosphate in vitro"

    Article Title: ParB spreading on DNA requires cytidine triphosphate in vitro

    Journal: bioRxiv

    doi: 10.1101/2019.12.11.865972

    Dual biotin-labeled DNA fragments form a closed substrate on the surface of the BLI probe. (A) A schematic of a double digestion assay using Exonuclease T7 + Exonuclease VII and PCR. PCR was performed using M13F, M13R oligos, and DNA attached to the BLI surface as a template. (B) The BLI probe was severed from the plastic adaptor and immerged into a PCR master mix. (C) Dual biotin-labeled DNA fragments on the BLI surface were resistant to Exo T7 + Exo VII digestion while single biotin-labeled DNA fragments on the BLI surface were not.
    Figure Legend Snippet: Dual biotin-labeled DNA fragments form a closed substrate on the surface of the BLI probe. (A) A schematic of a double digestion assay using Exonuclease T7 + Exonuclease VII and PCR. PCR was performed using M13F, M13R oligos, and DNA attached to the BLI surface as a template. (B) The BLI probe was severed from the plastic adaptor and immerged into a PCR master mix. (C) Dual biotin-labeled DNA fragments on the BLI surface were resistant to Exo T7 + Exo VII digestion while single biotin-labeled DNA fragments on the BLI surface were not.

    Techniques Used: Labeling, Polymerase Chain Reaction

    Related Articles

    Amplification:

    Article Title: Stochasticity enables BCR-independent germinal center initiation and antibody affinity maturation
    Article Snippet: .. The barcoding program was as follows: 98°C for 1 min, 55°C for 30 s, 72°C for 10 min. Extraneous barcode primers were removed with 1 µl Exonuclease VII (New England Biolabs) per 40-µl reaction, incubated at 37°C for 1 h, followed by heat-inactivation at 95°C for 15 min. First-round PCR was designed to add adaptors at the 5′ and 3′ ends of the amplicon. ..

    Selection:

    Article Title: High-Resolution Analysis of the Efficiency, Heritability, and Editing Outcomes of CRISPR/Cas9-Induced Modifications of NCED4 in Lettuce (Lactuca sativa)
    Article Snippet: .. Given the recent concerns of index switching during sequencing of dual index multiplexed samples using Illumina platforms , the pooled libraries were treated with Exonuclease VII (NEB, Ipswich, MA) to remove all residual single-stranded index primers, after which we performed a BluePippin (Sage Science Inc., Beverly, MA) size selection treatment for removal of all fragments smaller than 100 bp. .. After sequencing, we analyzed the occurrence of index switching in our datasets by demultiplexing the unused index combinations and mapping the reads to the reference.

    Ligation:

    Article Title: A flexible and efficient template format for circular consensus sequencing and SNP detection
    Article Snippet: .. Failed ligation products were removed through digestion in the presence of ExoIII and ExoVII exonucleases (NEB and USB, respectively). ..

    Polymerase Chain Reaction:

    Article Title: Stochasticity enables BCR-independent germinal center initiation and antibody affinity maturation
    Article Snippet: .. The barcoding program was as follows: 98°C for 1 min, 55°C for 30 s, 72°C for 10 min. Extraneous barcode primers were removed with 1 µl Exonuclease VII (New England Biolabs) per 40-µl reaction, incubated at 37°C for 1 h, followed by heat-inactivation at 95°C for 15 min. First-round PCR was designed to add adaptors at the 5′ and 3′ ends of the amplicon. ..

    Incubation:

    Article Title: Stochasticity enables BCR-independent germinal center initiation and antibody affinity maturation
    Article Snippet: .. The barcoding program was as follows: 98°C for 1 min, 55°C for 30 s, 72°C for 10 min. Extraneous barcode primers were removed with 1 µl Exonuclease VII (New England Biolabs) per 40-µl reaction, incubated at 37°C for 1 h, followed by heat-inactivation at 95°C for 15 min. First-round PCR was designed to add adaptors at the 5′ and 3′ ends of the amplicon. ..

    Formalin-fixed Paraffin-Embedded:

    Article Title: Large Enriched Fragment Targeted Sequencing (LEFT-SEQ) Applied to Capture of Wolbachia Genomes
    Article Snippet: .. PacBio library preparation A preliminary step of single strand removal and DNA damage repair was performed using Exonuclease VII and the NEBNext® FFPE DNA Repair kit (New England Biolabs, MA, US) in a 48 μL reaction volume containing: the captured DNA (~500 ng), 5 μL of NEBNext® FFPE DNA repair buffer and 1 μL of exonuclease VII (NEB) (Fig. ). .. 2 μL of NEBNext® FFPE DNA Enzyme mix was added to the reaction and incubated 20 minutes at 37 °C.

    Sequencing:

    Article Title: High-Resolution Analysis of the Efficiency, Heritability, and Editing Outcomes of CRISPR/Cas9-Induced Modifications of NCED4 in Lettuce (Lactuca sativa)
    Article Snippet: .. Given the recent concerns of index switching during sequencing of dual index multiplexed samples using Illumina platforms , the pooled libraries were treated with Exonuclease VII (NEB, Ipswich, MA) to remove all residual single-stranded index primers, after which we performed a BluePippin (Sage Science Inc., Beverly, MA) size selection treatment for removal of all fragments smaller than 100 bp. .. After sequencing, we analyzed the occurrence of index switching in our datasets by demultiplexing the unused index combinations and mapping the reads to the reference.

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  • 97
    New England Biolabs exonuclease vii
    Dual biotin-labeled DNA fragments form a closed substrate on the surface of the <t>BLI</t> probe. (A) A schematic of a double digestion assay using Exonuclease T7 + Exonuclease <t>VII</t> and PCR. PCR was performed using M13F, M13R oligos, and DNA attached to the BLI surface as a template. (B) The BLI probe was severed from the plastic adaptor and immerged into a PCR master mix. (C) Dual biotin-labeled DNA fragments on the BLI surface were resistant to Exo T7 + Exo VII digestion while single biotin-labeled DNA fragments on the BLI surface were not.
    Exonuclease Vii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exonuclease vii/product/New England Biolabs
    Average 97 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    exonuclease vii - by Bioz Stars, 2020-09
    97/100 stars
      Buy from Supplier

    Image Search Results


    Dual biotin-labeled DNA fragments form a closed substrate on the surface of the BLI probe. (A) A schematic of a double digestion assay using Exonuclease T7 + Exonuclease VII and PCR. PCR was performed using M13F, M13R oligos, and DNA attached to the BLI surface as a template. (B) The BLI probe was severed from the plastic adaptor and immerged into a PCR master mix. (C) Dual biotin-labeled DNA fragments on the BLI surface were resistant to Exo T7 + Exo VII digestion while single biotin-labeled DNA fragments on the BLI surface were not.

    Journal: bioRxiv

    Article Title: ParB spreading on DNA requires cytidine triphosphate in vitro

    doi: 10.1101/2019.12.11.865972

    Figure Lengend Snippet: Dual biotin-labeled DNA fragments form a closed substrate on the surface of the BLI probe. (A) A schematic of a double digestion assay using Exonuclease T7 + Exonuclease VII and PCR. PCR was performed using M13F, M13R oligos, and DNA attached to the BLI surface as a template. (B) The BLI probe was severed from the plastic adaptor and immerged into a PCR master mix. (C) Dual biotin-labeled DNA fragments on the BLI surface were resistant to Exo T7 + Exo VII digestion while single biotin-labeled DNA fragments on the BLI surface were not.

    Article Snippet: To verify that dual biotin-labeled DNA fragments formed a closed substrate on the surface of the BLI probe, we performed a double digestion with Exonuclease T7 and Exonuclease VII (NEB) ( ).

    Techniques: Labeling, Polymerase Chain Reaction