exovii  (New England Biolabs)


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  • 96
    Name:
    Exonuclease VII
    Description:
    Exonuclease VII 1 000 units
    Catalog Number:
    M0379L
    Price:
    640
    Category:
    Exonucleases
    Size:
    1 000 units
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    Structured Review

    New England Biolabs exovii
    Exonuclease VII
    Exonuclease VII 1 000 units
    https://www.bioz.com/result/exovii/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    exovii - by Bioz Stars, 2021-06
    96/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: RNase H-dependent PCR enables highly specific amplification of antibody variable domains from single B-cells
    Article Snippet: The plates were incubated at 37°C and at timepoints of 1 hour, 3 hours, and 6 hours one plate was removed from incubation and 2 μL of the reactions were analyzed by capillary electrophoresis as described above. .. Exonuclease digestion of PCR productsTo digest the unincorporated primer pools in the completed PCR reactions prior to capillary electrophoresis, 5 μL of PCR product was transferred to wells of a 96-well PCR plate and 1 μL of either Exonuclease I (20U/μL)(NEB) or Exonuclease VII (10U/μL)(NEB) were added. ..

    Electrophoresis:

    Article Title: RNase H-dependent PCR enables highly specific amplification of antibody variable domains from single B-cells
    Article Snippet: The plates were incubated at 37°C and at timepoints of 1 hour, 3 hours, and 6 hours one plate was removed from incubation and 2 μL of the reactions were analyzed by capillary electrophoresis as described above. .. Exonuclease digestion of PCR productsTo digest the unincorporated primer pools in the completed PCR reactions prior to capillary electrophoresis, 5 μL of PCR product was transferred to wells of a 96-well PCR plate and 1 μL of either Exonuclease I (20U/μL)(NEB) or Exonuclease VII (10U/μL)(NEB) were added. ..

    other:

    Article Title: ParB spreading on DNA requires cytidine triphosphate in vitro
    Article Snippet: To verify that dual biotin-labeled DNA fragments formed a closed substrate on the surface of the BLI probe, we performed a double digestion with Exonuclease T7 and Exonuclease VII (NEB) ( ).

    Incubation:

    Article Title: Large Enriched Fragment Targeted Sequencing (LEFT-SEQ) Applied to Capture of Wolbachia Genomes
    Article Snippet: Next, PacBio Blunt Adapters were ligated in a 40 μL reaction volume containing the end repaired reaction mixture of the previous step using 0.5 μM Annealed PacBio Blunt Adapters, 1X NEB T4 Ligase buffer and 2,000 units of T4 DNA Ligase. .. 100 units of Exonuclease III (NEB) and 10 units of Exonuclease VII NEB) were added to the reaction and incubated 37 °C for 45 minutes. ..

    Article Title: Novel CRISPR-based sequence specific enrichment methods for target loci and single base mutations
    Article Snippet: The Cas9/sgRNA complexes were then mixed with target DNA and incubated for 60 minutes at 37°C. .. Next, exonuclease III (NEB, cat. #M0206L) and exonuclease VII (NEB, cat. #M0379L) were added with NEBuffer 1 (NEB, cat. #B7001S) and incubated for a total of 240 minutes at 37°C. .. Genomic DNA experiments used 200 ng DNA (Horizon Discovery, cat. #HD272; Promega cat. #G3041) and cfDNA enrichment experiments used 200 ng of Horizon Discovery Multiplex I DNA (cat. #HD780).

    Ligation:

    Article Title: Dual histone methyl reader ZCWPW1 facilitates repair of meiotic double strand breaks in male mice
    Article Snippet: .. Enzymatic reactionsPlugs were treated with sequential combination of Exonuclease VII (NEB) for 1 hr at 37°C followed by Exonuclease T (NEB) for 45 min at 24°C to blunt DNA ends before Illumina adapter ligation ( ). .. Subsequent steps of A-tailing, adapter ligation, plug melting, chromatin shearing, and second round of adapter ligation for sequencing were performed exactly as previously described ( ; ).

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  • 96
    New England Biolabs exonuclease vii
    Dual biotin-labeled DNA fragments form a closed substrate on the surface of the <t>BLI</t> probe. (A) A schematic of a double digestion assay using Exonuclease T7 + Exonuclease <t>VII</t> and PCR. PCR was performed using M13F, M13R oligos, and DNA attached to the BLI surface as a template. (B) The BLI probe was severed from the plastic adaptor and immerged into a PCR master mix. (C) Dual biotin-labeled DNA fragments on the BLI surface were resistant to Exo T7 + Exo VII digestion while single biotin-labeled DNA fragments on the BLI surface were not.
    Exonuclease Vii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exonuclease vii/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    exonuclease vii - by Bioz Stars, 2021-06
    96/100 stars
      Buy from Supplier

    Image Search Results


    Dual biotin-labeled DNA fragments form a closed substrate on the surface of the BLI probe. (A) A schematic of a double digestion assay using Exonuclease T7 + Exonuclease VII and PCR. PCR was performed using M13F, M13R oligos, and DNA attached to the BLI surface as a template. (B) The BLI probe was severed from the plastic adaptor and immerged into a PCR master mix. (C) Dual biotin-labeled DNA fragments on the BLI surface were resistant to Exo T7 + Exo VII digestion while single biotin-labeled DNA fragments on the BLI surface were not.

    Journal: bioRxiv

    Article Title: ParB spreading on DNA requires cytidine triphosphate in vitro

    doi: 10.1101/2019.12.11.865972

    Figure Lengend Snippet: Dual biotin-labeled DNA fragments form a closed substrate on the surface of the BLI probe. (A) A schematic of a double digestion assay using Exonuclease T7 + Exonuclease VII and PCR. PCR was performed using M13F, M13R oligos, and DNA attached to the BLI surface as a template. (B) The BLI probe was severed from the plastic adaptor and immerged into a PCR master mix. (C) Dual biotin-labeled DNA fragments on the BLI surface were resistant to Exo T7 + Exo VII digestion while single biotin-labeled DNA fragments on the BLI surface were not.

    Article Snippet: To verify that dual biotin-labeled DNA fragments formed a closed substrate on the surface of the BLI probe, we performed a double digestion with Exonuclease T7 and Exonuclease VII (NEB) ( ).

    Techniques: Labeling, Polymerase Chain Reaction

    Characterization of rh-PCR Gen-2 primers. (A) Incubation of the VL rh-PCR Gen-2 primer pool with Exonuclease I or VII. (B) Amplification of VH fragments using standard FR1 and J-region PCR primers and the rh-PCR Gen-1 and rh-PCR Gen-2 derivatives. The electropherogram is on the left and a gel pseudo-image on the right. UM- CE Upper Marker, LM- CE Lower Marker, VH- variable region PCR product, PP- unused Primer Pool, U- Unwanted products.

    Journal: PLoS ONE

    Article Title: RNase H-dependent PCR enables highly specific amplification of antibody variable domains from single B-cells

    doi: 10.1371/journal.pone.0241803

    Figure Lengend Snippet: Characterization of rh-PCR Gen-2 primers. (A) Incubation of the VL rh-PCR Gen-2 primer pool with Exonuclease I or VII. (B) Amplification of VH fragments using standard FR1 and J-region PCR primers and the rh-PCR Gen-1 and rh-PCR Gen-2 derivatives. The electropherogram is on the left and a gel pseudo-image on the right. UM- CE Upper Marker, LM- CE Lower Marker, VH- variable region PCR product, PP- unused Primer Pool, U- Unwanted products.

    Article Snippet: Exonuclease digestion of PCR productsTo digest the unincorporated primer pools in the completed PCR reactions prior to capillary electrophoresis, 5 μL of PCR product was transferred to wells of a 96-well PCR plate and 1 μL of either Exonuclease I (20U/μL)(NEB) or Exonuclease VII (10U/μL)(NEB) were added.

    Techniques: Polymerase Chain Reaction, Incubation, Amplification, Marker