m0372s  (New England Biolabs)


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  • 98
    Name:
    Antarctic Thermolabile UDG
    Description:
    Antarctic Thermolabile UDG 500 units
    Catalog Number:
    M0372L
    Price:
    310
    Category:
    DNA Glycosylases
    Size:
    500 units
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    Structured Review

    New England Biolabs m0372s
    Antarctic Thermolabile UDG
    Antarctic Thermolabile UDG 500 units
    https://www.bioz.com/result/m0372s/product/New England Biolabs
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m0372s - by Bioz Stars, 2021-06
    98/100 stars

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    Related Articles

    other:

    Article Title: Ultra-rapid detection of SARS-CoV-2 in public workspace environments
    Article Snippet: DP-RT-LAMP assay12.5 μL of 2X WarmStart LAMP master mix (NEB) was combined with 2.5 μL of 10X LAMP primer set, 1 μL of excess B3 primer (300 μM), 0.2 μL of dUTP (100 mM, Promega), 0.5 μL of Antarctic Thermolabile UDG (1U/μL, NEB), 0.5 μL of RNase inhibitor (40U/μL, NEB), and 8 μL of sample (consisting of either heat-inactivated virus or nasal/saliva samples spiked with heat-inactivated virus).

    Article Title: Prediction of blood-based biomarkers and subsequent design of bisulfite PCR-LDR-qPCR assay for breast cancer detection
    Article Snippet: The mixture included 2 μl of diluted LDR product (Step 3), 1X Luna Universal ProbeqPCR master mixture, 0.1% tween 20, 0.4 mU RNAseH2, 0.025 U Antarctic Thermolabile UDG, 5 μM each of forward and reverse primers, and Taqman probe.

    Article Title: End-to-End Protocol for the Detection of SARS-CoV-2 from Built Environments
    Article Snippet: The final 20-μl reaction mix also included Antarctic thermolabile UDG (uracil-DNA glycosylase) to prevent sample cross-contamination.

    Article Title: Point of sampling detection of Zika virus within a multiplexed kit capable of detecting dengue and chikungunya
    Article Snippet: Managing forward contamination Carryover contamination was prevented by incorporation of dUTP by Bst 2.0 WarmStart® DNA Polymerase (NEB, Ipswich, MA) during RT-LAMP, and Antarctic thermolabile UDG (NEB, Ipswich, MA) was used to destroy DNA containing dU.

    Incubation:

    Article Title: A versatile method for circulating cell-free DNA methylome profiling by reduced representation bisulfite sequencing
    Article Snippet: The next day, a mixture of 0.5 µL of exonuclease I (20 U/µL) (NEB, Cat. No. M0293S), 0.5 µL of exonuclease III (50 U/µL) (NEB, Cat. No. M0206S), 0.5 µL of exonuclease VII (10U/µL) (NEB, Cat. No. M0379S), 0.5 µL of 10X CutSmart and 3 µL of H2 O was pipetted to the 40µL-reaction. .. As a last step before bisulfite conversion, 1 µL of Antarctic Thermolabile UDG (1U/µL) (NEB, Cat. No. M0372S), 0.5 µL of endonuclease VIII (10U/µL) (NEB, Cat. No. M0299S), 0.5 µL of 10X CutSmart and 3 µL of H2O was added and incubated for 1 hour at 37°C and heat killed for 10 minutes at 75°C. .. The MspI/MspI-digested adapter-ligated DNA fragments were bisulfite converted using the EZ DNA Methylation-Lightning™ Kit according to manufacturer’s instructions.

    Recombinant:

    Article Title: Cold and Hot Extremozymes: Industrial Relevance and Current Trends
    Article Snippet: .. Additionally, New England Biolabs offers Antarctic Thermolabile UDG, a recombinant UNG enzyme produced in E. coli from a psychrophilic marine bacterium with an inactivation temperature above 50°C. (b) Double strand-specific DNase allows for the digestion of double-stranded DNA without any effect on single-stranded DNA molecules, such as primers or probes. .. This enzyme is used to decontaminate PCR mixes or to remove genomic DNA from RNA preparations.

    Produced:

    Article Title: Cold and Hot Extremozymes: Industrial Relevance and Current Trends
    Article Snippet: .. Additionally, New England Biolabs offers Antarctic Thermolabile UDG, a recombinant UNG enzyme produced in E. coli from a psychrophilic marine bacterium with an inactivation temperature above 50°C. (b) Double strand-specific DNase allows for the digestion of double-stranded DNA without any effect on single-stranded DNA molecules, such as primers or probes. .. This enzyme is used to decontaminate PCR mixes or to remove genomic DNA from RNA preparations.

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  • 98
    New England Biolabs antarctic thermolabile udg
    Antarctic Thermolabile Udg, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antarctic thermolabile udg/product/New England Biolabs
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antarctic thermolabile udg - by Bioz Stars, 2021-06
    98/100 stars
      Buy from Supplier

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