shrimp hepatopancreas alp  (New England Biolabs)


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    Name:
    Shrimp Alkaline Phosphatase rSAP
    Description:
    Shrimp Alkaline Phosphatase rSAP 2 500 units
    Catalog Number:
    M0371L
    Price:
    248
    Category:
    Alkaline Phosphatases
    Size:
    2 500 units
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    Structured Review

    New England Biolabs shrimp hepatopancreas alp
    Shrimp Alkaline Phosphatase rSAP
    Shrimp Alkaline Phosphatase rSAP 2 500 units
    https://www.bioz.com/result/shrimp hepatopancreas alp/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    shrimp hepatopancreas alp - by Bioz Stars, 2021-05
    99/100 stars

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    1) Product Images from "Innate Biomineralization"

    Article Title: Innate Biomineralization

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms21144820

    Calcium ion (Ca 2+ ), a phosphoester salt, and alkaline phosphatase (ALP) are essential for promoting biomineralization in MG-63 and Saos-2 cell lines and human blood mononuclear cells (MNCs). ( A ) Alizarin Red S assays (ARS) show mineralization within 7 days, visualized as the intensity of red staining, of Saos-2 cells in MEMα/10% FBS supplemented only with βGP or the combination of βGP/ascorbic acid (Vit. C)/dexamethasone (Dex); MG-63 cell line was inactive under similar conditions. No mineralization was observed in either cell line cultured with MEMα/10% FBS supplemented with Vit. C or Dex. In MEMα/10% FBS supplemented with βGP and ALPL, calf intestinal ALP (CIP), or shrimp ALP (SAP), MG-63 and Saos-2 cell lines were mineralized. ( B ) Similar results were iterated when αGP was used instead of βGP. ( C ) Compared to βGP and phosphoenolpyruvate monosodium (PEP), αGP was the most efficient phosphoester salt elicited biomineralization. Pamidronate (Pamidn) and glycerophosphoric acid (NSC9231) did not elicit the reaction. ( D ) Human MNCs also have an innate ability of mineralization in 7 days without the induction of cellular differentiation (top row indicates initial cell counts per well in a 6-well plate). ( E – G ) Titration assays indicated that biomineralization depended on the doses of αGP, CIP, and Ca 2+ . ( H ) In a 48-well plate, human blood MNCs were seeded (10 5 /well) and exposed to MEMα/10% FBS, the medium supplemented with αGP, with αGP and CIP, or with CIP for 7 days (the media were changed on day 4). On day 7, ARS indicates that biomineralization occurred in MNCs exposed to MEMα/10% FBS supplemented with αGP and CIP in the uncoated wells (top row) and the wells coated with Collagen Type I, rat tail (middle row). Biomineralization did not occur if αGP or CIP was missing. None of the cell-less wells coated with Collagen Type I was positive for HAP by ARS (bottom row). Each of the tests was in duplicates.
    Figure Legend Snippet: Calcium ion (Ca 2+ ), a phosphoester salt, and alkaline phosphatase (ALP) are essential for promoting biomineralization in MG-63 and Saos-2 cell lines and human blood mononuclear cells (MNCs). ( A ) Alizarin Red S assays (ARS) show mineralization within 7 days, visualized as the intensity of red staining, of Saos-2 cells in MEMα/10% FBS supplemented only with βGP or the combination of βGP/ascorbic acid (Vit. C)/dexamethasone (Dex); MG-63 cell line was inactive under similar conditions. No mineralization was observed in either cell line cultured with MEMα/10% FBS supplemented with Vit. C or Dex. In MEMα/10% FBS supplemented with βGP and ALPL, calf intestinal ALP (CIP), or shrimp ALP (SAP), MG-63 and Saos-2 cell lines were mineralized. ( B ) Similar results were iterated when αGP was used instead of βGP. ( C ) Compared to βGP and phosphoenolpyruvate monosodium (PEP), αGP was the most efficient phosphoester salt elicited biomineralization. Pamidronate (Pamidn) and glycerophosphoric acid (NSC9231) did not elicit the reaction. ( D ) Human MNCs also have an innate ability of mineralization in 7 days without the induction of cellular differentiation (top row indicates initial cell counts per well in a 6-well plate). ( E – G ) Titration assays indicated that biomineralization depended on the doses of αGP, CIP, and Ca 2+ . ( H ) In a 48-well plate, human blood MNCs were seeded (10 5 /well) and exposed to MEMα/10% FBS, the medium supplemented with αGP, with αGP and CIP, or with CIP for 7 days (the media were changed on day 4). On day 7, ARS indicates that biomineralization occurred in MNCs exposed to MEMα/10% FBS supplemented with αGP and CIP in the uncoated wells (top row) and the wells coated with Collagen Type I, rat tail (middle row). Biomineralization did not occur if αGP or CIP was missing. None of the cell-less wells coated with Collagen Type I was positive for HAP by ARS (bottom row). Each of the tests was in duplicates.

    Techniques Used: Staining, Cell Culture, Cell Differentiation, Titration

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Regulated post-transcriptional RNA cleavage diversifies the eukaryotic transcriptome
    Article Snippet: RNA was then reverse-transcribed (SuperscriptIII; Invitrogen) using the primer [5′-CGAGCACAGAATTAATACGACT(18)V-3′] and amplified by PCR (Phusion DNA polymerase; Finnzymes) using the primers (5′-GTTCAGAGTTCTACAGTCCGAC-3′ and 5′-CGAGCACAGAATTAATACGAC-3′). .. PCR conditions were seven cycles of 94°C for 30 sec, 60°C for 20 sec, and 72°C for 3 min. Products were gel-purified, cleaved with MmeI (New England Biolabs), and dephosphorylated (shrimp alkaline phosphatase; New England Biolabs). ..

    Article Title: Genome-wide analyses reveal lineage specific contributions of positive selection and recombination to the evolution of Listeria monocytogenes
    Article Snippet: PCR amplification of the five selected genes was carried out using primers and conditions described in Additional file . .. PCR fragments were purified using Exonuclease I (0.5 U/μl) and Shrimp alkaline phosphatase (0.05 U/μl) (USB, NEB) and sequenced (at the Biotechnology Resource Center, Cornell University) using Big Dye Terminator chemistry and AmpliTaq-FS DNA Polymerase and an automated 3730 DNA Analyzer. ..

    Article Title: Integron Diversity in Heavy-Metal-Contaminated Mine Tailings and Inferences about Integron Evolution
    Article Snippet: .. Inserted sequences were PCR amplified using the primers M13F and M13R and cleaned using exonuclease I and shrimp alkaline phosphatase (New England Biolabs, Beverly, Mass.) or QIAquick PCR purification columns (Qiagen, Valencia, Calif.). ..

    Size-exclusion Chromatography:

    Article Title: Regulated post-transcriptional RNA cleavage diversifies the eukaryotic transcriptome
    Article Snippet: RNA was then reverse-transcribed (SuperscriptIII; Invitrogen) using the primer [5′-CGAGCACAGAATTAATACGACT(18)V-3′] and amplified by PCR (Phusion DNA polymerase; Finnzymes) using the primers (5′-GTTCAGAGTTCTACAGTCCGAC-3′ and 5′-CGAGCACAGAATTAATACGAC-3′). .. PCR conditions were seven cycles of 94°C for 30 sec, 60°C for 20 sec, and 72°C for 3 min. Products were gel-purified, cleaved with MmeI (New England Biolabs), and dephosphorylated (shrimp alkaline phosphatase; New England Biolabs). ..

    Purification:

    Article Title: Acrylonitrile‐Mediated Nascent RNA Sequencing for Transcriptome‐Wide Profiling of Cellular RNA Dynamics, Acrylonitrile‐Mediated Nascent RNA Sequencing for Transcriptome‐Wide Profiling of Cellular RNA Dynamics
    Article Snippet: .. To enzymatically degrade RNA to monomeric ribonucleosides, Nuclease P1 (2 U, Sigma) was added to a 50 µL solution containing 2 mm ZnCl2 , 10 mm NaCl, 100 µm DTT, and 600 ng purified RNA and incubated at 37 °C for 2 h. Then, 6 µL 200 mm Tris‐HCl (pH 7.9), 1 µL 1 mm DTT, 3 µL 100 mm MgCl2 , and 2 µL Shrimp Alkaline Phosphatase (2 U, NEB) were added to the reaction solution and incubated for another 2 h at 37 °C. .. Each sample was centrifuged at 12 000 g at 4 °C for 20 min, 50 µL supernatant was collected and directly subjected to HPLC‐MS analysis. s4 U and ces4 U ribonucleosides which diluted in enzymatic buffer containing a mixture of four common bases were used to make a standard curve for quantitative MS analysis.

    Article Title: Genome-wide analyses reveal lineage specific contributions of positive selection and recombination to the evolution of Listeria monocytogenes
    Article Snippet: PCR amplification of the five selected genes was carried out using primers and conditions described in Additional file . .. PCR fragments were purified using Exonuclease I (0.5 U/μl) and Shrimp alkaline phosphatase (0.05 U/μl) (USB, NEB) and sequenced (at the Biotechnology Resource Center, Cornell University) using Big Dye Terminator chemistry and AmpliTaq-FS DNA Polymerase and an automated 3730 DNA Analyzer. ..

    Article Title: Integron Diversity in Heavy-Metal-Contaminated Mine Tailings and Inferences about Integron Evolution
    Article Snippet: .. Inserted sequences were PCR amplified using the primers M13F and M13R and cleaned using exonuclease I and shrimp alkaline phosphatase (New England Biolabs, Beverly, Mass.) or QIAquick PCR purification columns (Qiagen, Valencia, Calif.). ..

    Incubation:

    Article Title: Acrylonitrile‐Mediated Nascent RNA Sequencing for Transcriptome‐Wide Profiling of Cellular RNA Dynamics, Acrylonitrile‐Mediated Nascent RNA Sequencing for Transcriptome‐Wide Profiling of Cellular RNA Dynamics
    Article Snippet: .. To enzymatically degrade RNA to monomeric ribonucleosides, Nuclease P1 (2 U, Sigma) was added to a 50 µL solution containing 2 mm ZnCl2 , 10 mm NaCl, 100 µm DTT, and 600 ng purified RNA and incubated at 37 °C for 2 h. Then, 6 µL 200 mm Tris‐HCl (pH 7.9), 1 µL 1 mm DTT, 3 µL 100 mm MgCl2 , and 2 µL Shrimp Alkaline Phosphatase (2 U, NEB) were added to the reaction solution and incubated for another 2 h at 37 °C. .. Each sample was centrifuged at 12 000 g at 4 °C for 20 min, 50 µL supernatant was collected and directly subjected to HPLC‐MS analysis. s4 U and ces4 U ribonucleosides which diluted in enzymatic buffer containing a mixture of four common bases were used to make a standard curve for quantitative MS analysis.

    Article Title: 3′ end additions by T7 RNA polymerase are RNA self-templated, distributive and diverse in character—RNA-Seq analyses
    Article Snippet: Library preparation for RNA-Seq As outlined in , transcripts from all reactions were incubated at 37°C for 1 h with 5′ Pyrophosphohydrolase/RppH (New England BioLabs) to remove pyrophosphate from the 5′ end of tri-phosphorylated RNAs and to generate 5′ monophosphate RNAs. .. Transcripts were then incubated with Shrimp Alkaline Phosphatase/rSAP (New England BioLabs) at 37°C for 45 min to dephosphorylate the 5′ end of RNAs and to hydrolyze remaining NTPs from the reaction. .. Afterward, phosphatase activity was eliminated by heat inactivation at 65°C for 5 min.

    Amplification:

    Article Title: Integron Diversity in Heavy-Metal-Contaminated Mine Tailings and Inferences about Integron Evolution
    Article Snippet: .. Inserted sequences were PCR amplified using the primers M13F and M13R and cleaned using exonuclease I and shrimp alkaline phosphatase (New England Biolabs, Beverly, Mass.) or QIAquick PCR purification columns (Qiagen, Valencia, Calif.). ..

    other:

    Article Title: Extensive transcriptional heterogeneity revealed by isoform profiling
    Article Snippet: RNA was dephosphorylated using Shrimp Alkaline Phosphatase as described earlier, but instead of proceeding to treatment with Tobacco Acid Pyrophosphatase, RNA was rephosphorylated for 1 h at 37°C using T4 Polynucleotide Kinase (NEB).

    High Performance Liquid Chromatography:

    Article Title: Nucleotide resolution profiling of m3C RNA modification by HAC-seq
    Article Snippet: .. HPLC-MS/MS analysis of RNA 250 ng to 500 ng RNA was digested with 100 U S1 nuclease (Thermo-Fisher # EN0321) at 37°C for 2 h and dephosphorylated with 1 U rSAP (NEB # M0371S) at 37°C for 1 h. The 100 μl samples were filtered with Millex-GV 0.22u filters (Millipore Sigma # SLGV033RS). .. 5–10 μl from each sample was injected into the Agilent 6470 Triple Quad LC/MS instrument with Agilent Zorbax Eclipse C18 reverse phase HPLC column.

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  • 99
    New England Biolabs shrimp hepatopancreas alp
    Calcium ion (Ca 2+ ), a phosphoester salt, and alkaline phosphatase <t>(ALP)</t> are essential for promoting biomineralization in MG-63 and Saos-2 cell lines and human blood mononuclear cells (MNCs). ( A ) Alizarin Red S assays (ARS) show mineralization within 7 days, visualized as the intensity of red staining, of Saos-2 cells in MEMα/10% FBS supplemented only with βGP or the combination of βGP/ascorbic acid (Vit. C)/dexamethasone (Dex); MG-63 cell line was inactive under similar conditions. No mineralization was observed in either cell line cultured with MEMα/10% FBS supplemented with Vit. C or Dex. In MEMα/10% FBS supplemented with βGP and ALPL, calf intestinal ALP <t>(CIP),</t> or shrimp ALP (SAP), MG-63 and Saos-2 cell lines were mineralized. ( B ) Similar results were iterated when αGP was used instead of βGP. ( C ) Compared to βGP and phosphoenolpyruvate monosodium (PEP), αGP was the most efficient phosphoester salt elicited biomineralization. Pamidronate (Pamidn) and glycerophosphoric acid (NSC9231) did not elicit the reaction. ( D ) Human MNCs also have an innate ability of mineralization in 7 days without the induction of cellular differentiation (top row indicates initial cell counts per well in a 6-well plate). ( E – G ) Titration assays indicated that biomineralization depended on the doses of αGP, CIP, and Ca 2+ . ( H ) In a 48-well plate, human blood MNCs were seeded (10 5 /well) and exposed to MEMα/10% FBS, the medium supplemented with αGP, with αGP and CIP, or with CIP for 7 days (the media were changed on day 4). On day 7, ARS indicates that biomineralization occurred in MNCs exposed to MEMα/10% FBS supplemented with αGP and CIP in the uncoated wells (top row) and the wells coated with Collagen Type I, rat tail (middle row). Biomineralization did not occur if αGP or CIP was missing. None of the cell-less wells coated with Collagen Type I was positive for HAP by ARS (bottom row). Each of the tests was in duplicates.
    Shrimp Hepatopancreas Alp, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/shrimp hepatopancreas alp/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    shrimp hepatopancreas alp - by Bioz Stars, 2021-05
    99/100 stars
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    Calcium ion (Ca 2+ ), a phosphoester salt, and alkaline phosphatase (ALP) are essential for promoting biomineralization in MG-63 and Saos-2 cell lines and human blood mononuclear cells (MNCs). ( A ) Alizarin Red S assays (ARS) show mineralization within 7 days, visualized as the intensity of red staining, of Saos-2 cells in MEMα/10% FBS supplemented only with βGP or the combination of βGP/ascorbic acid (Vit. C)/dexamethasone (Dex); MG-63 cell line was inactive under similar conditions. No mineralization was observed in either cell line cultured with MEMα/10% FBS supplemented with Vit. C or Dex. In MEMα/10% FBS supplemented with βGP and ALPL, calf intestinal ALP (CIP), or shrimp ALP (SAP), MG-63 and Saos-2 cell lines were mineralized. ( B ) Similar results were iterated when αGP was used instead of βGP. ( C ) Compared to βGP and phosphoenolpyruvate monosodium (PEP), αGP was the most efficient phosphoester salt elicited biomineralization. Pamidronate (Pamidn) and glycerophosphoric acid (NSC9231) did not elicit the reaction. ( D ) Human MNCs also have an innate ability of mineralization in 7 days without the induction of cellular differentiation (top row indicates initial cell counts per well in a 6-well plate). ( E – G ) Titration assays indicated that biomineralization depended on the doses of αGP, CIP, and Ca 2+ . ( H ) In a 48-well plate, human blood MNCs were seeded (10 5 /well) and exposed to MEMα/10% FBS, the medium supplemented with αGP, with αGP and CIP, or with CIP for 7 days (the media were changed on day 4). On day 7, ARS indicates that biomineralization occurred in MNCs exposed to MEMα/10% FBS supplemented with αGP and CIP in the uncoated wells (top row) and the wells coated with Collagen Type I, rat tail (middle row). Biomineralization did not occur if αGP or CIP was missing. None of the cell-less wells coated with Collagen Type I was positive for HAP by ARS (bottom row). Each of the tests was in duplicates.

    Journal: International Journal of Molecular Sciences

    Article Title: Innate Biomineralization

    doi: 10.3390/ijms21144820

    Figure Lengend Snippet: Calcium ion (Ca 2+ ), a phosphoester salt, and alkaline phosphatase (ALP) are essential for promoting biomineralization in MG-63 and Saos-2 cell lines and human blood mononuclear cells (MNCs). ( A ) Alizarin Red S assays (ARS) show mineralization within 7 days, visualized as the intensity of red staining, of Saos-2 cells in MEMα/10% FBS supplemented only with βGP or the combination of βGP/ascorbic acid (Vit. C)/dexamethasone (Dex); MG-63 cell line was inactive under similar conditions. No mineralization was observed in either cell line cultured with MEMα/10% FBS supplemented with Vit. C or Dex. In MEMα/10% FBS supplemented with βGP and ALPL, calf intestinal ALP (CIP), or shrimp ALP (SAP), MG-63 and Saos-2 cell lines were mineralized. ( B ) Similar results were iterated when αGP was used instead of βGP. ( C ) Compared to βGP and phosphoenolpyruvate monosodium (PEP), αGP was the most efficient phosphoester salt elicited biomineralization. Pamidronate (Pamidn) and glycerophosphoric acid (NSC9231) did not elicit the reaction. ( D ) Human MNCs also have an innate ability of mineralization in 7 days without the induction of cellular differentiation (top row indicates initial cell counts per well in a 6-well plate). ( E – G ) Titration assays indicated that biomineralization depended on the doses of αGP, CIP, and Ca 2+ . ( H ) In a 48-well plate, human blood MNCs were seeded (10 5 /well) and exposed to MEMα/10% FBS, the medium supplemented with αGP, with αGP and CIP, or with CIP for 7 days (the media were changed on day 4). On day 7, ARS indicates that biomineralization occurred in MNCs exposed to MEMα/10% FBS supplemented with αGP and CIP in the uncoated wells (top row) and the wells coated with Collagen Type I, rat tail (middle row). Biomineralization did not occur if αGP or CIP was missing. None of the cell-less wells coated with Collagen Type I was positive for HAP by ARS (bottom row). Each of the tests was in duplicates.

    Article Snippet: CIP (≥10,000 U/mL), shrimp hepatopancreas ALP (≥1000 unit/mL), and p-nitrophenyl phosphate kits were purchased from New England BioLabs (Ipswich, MA, USA).

    Techniques: Staining, Cell Culture, Cell Differentiation, Titration