electroligase  (New England Biolabs)


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  • 95
    Name:
    ElectroLigase
    Description:
    ElectroLigase 50 rxns
    Catalog Number:
    m0369s
    Price:
    121
    Size:
    50 rxns
    Category:
    DNA Ligases
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    Structured Review

    New England Biolabs electroligase
    ElectroLigase
    ElectroLigase 50 rxns
    https://www.bioz.com/result/electroligase/product/New England Biolabs
    Average 95 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    electroligase - by Bioz Stars, 2020-09
    95/100 stars

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    Related Articles

    TUNEL Assay:

    Article Title: RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer.
    Article Snippet: .. RUVBL1 and RUVBL2 (collectively RUVBL1/2) are essential AAA+ ATPases that function as co-chaperones and have been implicated in cancer. .. RUVBL1 and RUVBL2 (collectively RUVBL1/2) are essential AAA+ ATPases that function as co-chaperones and have been implicated in cancer.

    Electroporation:

    Article Title: A single bacterial genus maintains root development in a complex microbiome
    Article Snippet: .. The prepared Variovorax CL14 genomic DNA fragments were ligated to the prepared pBBR1-MCS2 vector backbone using ElectroLigase (New England Biolabs) and transformed by electroporation into NEB 10-beta Electrocompetent E. coli (New England Biolabs). .. Clones were selected by blue-white screening on LB plates containing 1.5% agar, 50 µg/mL kanamycin, 40 µg/mL X-gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside), and 1 mM Isopropyl β-d-1-thiogalactopyranoside (IPTG) at 37°C.

    Amplification:

    Article Title: RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer.
    Article Snippet: .. RUVBL1 and RUVBL2 (collectively RUVBL1/2) are essential AAA+ ATPases that function as co-chaperones and have been implicated in cancer. .. RUVBL1 and RUVBL2 (collectively RUVBL1/2) are essential AAA+ ATPases that function as co-chaperones and have been implicated in cancer.

    RNA Sequencing Assay:

    Article Title: RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer.
    Article Snippet: .. RUVBL1 and RUVBL2 (collectively RUVBL1/2) are essential AAA+ ATPases that function as co-chaperones and have been implicated in cancer. .. RUVBL1 and RUVBL2 (collectively RUVBL1/2) are essential AAA+ ATPases that function as co-chaperones and have been implicated in cancer.

    Electron Microscopy:

    Article Title: RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer.
    Article Snippet: .. RUVBL1 and RUVBL2 (collectively RUVBL1/2) are essential AAA+ ATPases that function as co-chaperones and have been implicated in cancer. .. RUVBL1 and RUVBL2 (collectively RUVBL1/2) are essential AAA+ ATPases that function as co-chaperones and have been implicated in cancer.

    Synthesized:

    Article Title: The next generation of biopanning: next gen sequencing improves analysis of bacterial display libraries
    Article Snippet: .. A DNA library of peptide inserts in the form of NNS within the eCPX3.0 gene was commercially synthesized (GeneArt), similarly digested and ligated into the vector backbone using electroligase (New England Biolabs). .. A total of 1.5 μ g of newly assembled constructs were then electroporated into 250 μ l electrically competent cells in batches of 1 μ l DNA with 25 μ l cells using a 0.1 cm gap, pre-chilled electroporation cuvette, and pulsed at 1.8 kV, 10 μ F, and 100 Ω .

    Construct:

    Article Title: Reconstruction of cysteine biosynthesis using engineered cysteine-free enzymes
    Article Snippet: .. Fifty nanograms of pUC19 and 50 ng of digested PCR products encoding the gene construct were mixed with ElectroLigase mix (New England Biolabs) in a final volume of 20 μl in 1x T4 DNA Ligase Reaction Buffer for 1 h. Ligated products were transformed into 50 µl of electrocompetent cells using an Electroporator 2510 (Eppendorf, Hauppauge, NY, USA) with an 1800V pulse. .. Cells were re-suspended in 500 μl of super optimal broth with catabolite repressor (SOC) medium (20 g tryptone, 0.5 g yeast extract, 0.5 g 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl2 , and 10 mM MgSO4 in 1 L of deionized water adjusted to a final pH 7.0) and incubated for 1 h at 37 °C before plating.

    Purification:

    Article Title: RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer.
    Article Snippet: .. RUVBL1 and RUVBL2 (collectively RUVBL1/2) are essential AAA+ ATPases that function as co-chaperones and have been implicated in cancer. .. RUVBL1 and RUVBL2 (collectively RUVBL1/2) are essential AAA+ ATPases that function as co-chaperones and have been implicated in cancer.

    SYBR Green Assay:

    Article Title: RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer.
    Article Snippet: .. RUVBL1 and RUVBL2 (collectively RUVBL1/2) are essential AAA+ ATPases that function as co-chaperones and have been implicated in cancer. .. RUVBL1 and RUVBL2 (collectively RUVBL1/2) are essential AAA+ ATPases that function as co-chaperones and have been implicated in cancer.

    DNA Sequencing:

    Article Title: RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer.
    Article Snippet: .. RUVBL1 and RUVBL2 (collectively RUVBL1/2) are essential AAA+ ATPases that function as co-chaperones and have been implicated in cancer. .. RUVBL1 and RUVBL2 (collectively RUVBL1/2) are essential AAA+ ATPases that function as co-chaperones and have been implicated in cancer.

    Polyacrylamide Gel Electrophoresis:

    Article Title: RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer.
    Article Snippet: .. RUVBL1 and RUVBL2 (collectively RUVBL1/2) are essential AAA+ ATPases that function as co-chaperones and have been implicated in cancer. .. RUVBL1 and RUVBL2 (collectively RUVBL1/2) are essential AAA+ ATPases that function as co-chaperones and have been implicated in cancer.

    Polymerase Chain Reaction:

    Article Title: CsrA and its regulators control the time-point of ColicinE2 release in Escherichia coli
    Article Snippet: .. After gel purification of the vector, both, the vector and the ORI p15A PCR fragment were cut with the enzymes SalI-HF and SphI-HF (NEB) and ligated in a 1:5 ratio of vector:insert using an ElectroLigase® (NEB). .. The resulting plasmid pMO8 was transformed into BZB 1011 and CWT, respectively, creating the reporter strains SREP2 and CREP2 (Table ).

    Article Title: RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer.
    Article Snippet: .. RUVBL1 and RUVBL2 (collectively RUVBL1/2) are essential AAA+ ATPases that function as co-chaperones and have been implicated in cancer. .. RUVBL1 and RUVBL2 (collectively RUVBL1/2) are essential AAA+ ATPases that function as co-chaperones and have been implicated in cancer.

    Article Title: Reconstruction of cysteine biosynthesis using engineered cysteine-free enzymes
    Article Snippet: .. Fifty nanograms of pUC19 and 50 ng of digested PCR products encoding the gene construct were mixed with ElectroLigase mix (New England Biolabs) in a final volume of 20 μl in 1x T4 DNA Ligase Reaction Buffer for 1 h. Ligated products were transformed into 50 µl of electrocompetent cells using an Electroporator 2510 (Eppendorf, Hauppauge, NY, USA) with an 1800V pulse. .. Cells were re-suspended in 500 μl of super optimal broth with catabolite repressor (SOC) medium (20 g tryptone, 0.5 g yeast extract, 0.5 g 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl2 , and 10 mM MgSO4 in 1 L of deionized water adjusted to a final pH 7.0) and incubated for 1 h at 37 °C before plating.

    Transformation Assay:

    Article Title: A single bacterial genus maintains root development in a complex microbiome
    Article Snippet: .. The prepared Variovorax CL14 genomic DNA fragments were ligated to the prepared pBBR1-MCS2 vector backbone using ElectroLigase (New England Biolabs) and transformed by electroporation into NEB 10-beta Electrocompetent E. coli (New England Biolabs). .. Clones were selected by blue-white screening on LB plates containing 1.5% agar, 50 µg/mL kanamycin, 40 µg/mL X-gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside), and 1 mM Isopropyl β-d-1-thiogalactopyranoside (IPTG) at 37°C.

    Article Title: Reconstruction of cysteine biosynthesis using engineered cysteine-free enzymes
    Article Snippet: .. Fifty nanograms of pUC19 and 50 ng of digested PCR products encoding the gene construct were mixed with ElectroLigase mix (New England Biolabs) in a final volume of 20 μl in 1x T4 DNA Ligase Reaction Buffer for 1 h. Ligated products were transformed into 50 µl of electrocompetent cells using an Electroporator 2510 (Eppendorf, Hauppauge, NY, USA) with an 1800V pulse. .. Cells were re-suspended in 500 μl of super optimal broth with catabolite repressor (SOC) medium (20 g tryptone, 0.5 g yeast extract, 0.5 g 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl2 , and 10 mM MgSO4 in 1 L of deionized water adjusted to a final pH 7.0) and incubated for 1 h at 37 °C before plating.

    Plasmid Preparation:

    Article Title: CsrA and its regulators control the time-point of ColicinE2 release in Escherichia coli
    Article Snippet: .. After gel purification of the vector, both, the vector and the ORI p15A PCR fragment were cut with the enzymes SalI-HF and SphI-HF (NEB) and ligated in a 1:5 ratio of vector:insert using an ElectroLigase® (NEB). .. The resulting plasmid pMO8 was transformed into BZB 1011 and CWT, respectively, creating the reporter strains SREP2 and CREP2 (Table ).

    Article Title: The next generation of biopanning: next gen sequencing improves analysis of bacterial display libraries
    Article Snippet: .. A DNA library of peptide inserts in the form of NNS within the eCPX3.0 gene was commercially synthesized (GeneArt), similarly digested and ligated into the vector backbone using electroligase (New England Biolabs). .. A total of 1.5 μ g of newly assembled constructs were then electroporated into 250 μ l electrically competent cells in batches of 1 μ l DNA with 25 μ l cells using a 0.1 cm gap, pre-chilled electroporation cuvette, and pulsed at 1.8 kV, 10 μ F, and 100 Ω .

    Article Title: A single bacterial genus maintains root development in a complex microbiome
    Article Snippet: .. The prepared Variovorax CL14 genomic DNA fragments were ligated to the prepared pBBR1-MCS2 vector backbone using ElectroLigase (New England Biolabs) and transformed by electroporation into NEB 10-beta Electrocompetent E. coli (New England Biolabs). .. Clones were selected by blue-white screening on LB plates containing 1.5% agar, 50 µg/mL kanamycin, 40 µg/mL X-gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside), and 1 mM Isopropyl β-d-1-thiogalactopyranoside (IPTG) at 37°C.

    Article Title: RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer.
    Article Snippet: .. RUVBL1 and RUVBL2 (collectively RUVBL1/2) are essential AAA+ ATPases that function as co-chaperones and have been implicated in cancer. .. RUVBL1 and RUVBL2 (collectively RUVBL1/2) are essential AAA+ ATPases that function as co-chaperones and have been implicated in cancer.

    Gel Purification:

    Article Title: CsrA and its regulators control the time-point of ColicinE2 release in Escherichia coli
    Article Snippet: .. After gel purification of the vector, both, the vector and the ORI p15A PCR fragment were cut with the enzymes SalI-HF and SphI-HF (NEB) and ligated in a 1:5 ratio of vector:insert using an ElectroLigase® (NEB). .. The resulting plasmid pMO8 was transformed into BZB 1011 and CWT, respectively, creating the reporter strains SREP2 and CREP2 (Table ).

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  • 95
    New England Biolabs electroligase
    Electroligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/electroligase/product/New England Biolabs
    Average 95 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    electroligase - by Bioz Stars, 2020-09
    95/100 stars
      Buy from Supplier

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